Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43972.t1 | XP_003590981.1 | 99 | 312 | 3 | 0 | 1 | 312 | 249 | 560 | 1.10E-147 | 532.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43972.t1 | G7I6T7 | 99.0 | 312 | 3 | 0 | 1 | 312 | 249 | 560 | 8.1e-148 | 532.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene43972 | 0.802676 | 5.01E-49 | -1.69E-46 |
| MS.gene04918 | MS.gene43972 | 0.812729 | 3.66E-51 | -1.69E-46 |
| MS.gene04920 | MS.gene43972 | 0.801252 | 9.83E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene43972 | 0.842467 | 2.54E-58 | -1.69E-46 |
| MS.gene049283 | MS.gene43972 | 0.829393 | 5.28E-55 | -1.69E-46 |
| MS.gene049368 | MS.gene43972 | 0.800738 | 1.25E-48 | -1.69E-46 |
| MS.gene049452 | MS.gene43972 | 0.802597 | 5.20E-49 | -1.69E-46 |
| MS.gene049499 | MS.gene43972 | 0.847518 | 1.10E-59 | -1.69E-46 |
| MS.gene049711 | MS.gene43972 | 0.827686 | 1.36E-54 | -1.69E-46 |
| MS.gene049983 | MS.gene43972 | 0.846616 | 1.94E-59 | -1.69E-46 |
| MS.gene050559 | MS.gene43972 | 0.842482 | 2.52E-58 | -1.69E-46 |
| MS.gene050561 | MS.gene43972 | 0.837871 | 4.02E-57 | -1.69E-46 |
| MS.gene050562 | MS.gene43972 | 0.840024 | 1.12E-57 | -1.69E-46 |
| MS.gene050646 | MS.gene43972 | 0.814721 | 1.33E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene43972 | 0.801788 | 7.63E-49 | -1.69E-46 |
| MS.gene051209 | MS.gene43972 | 0.8122 | 4.78E-51 | -1.69E-46 |
| MS.gene051279 | MS.gene43972 | 0.84418 | 8.87E-59 | -1.69E-46 |
| MS.gene051332 | MS.gene43972 | 0.81504 | 1.13E-51 | -1.69E-46 |
| MS.gene051744 | MS.gene43972 | 0.836627 | 8.38E-57 | -1.69E-46 |
| MS.gene051800 | MS.gene43972 | 0.815419 | 9.34E-52 | -1.69E-46 |
| MS.gene052037 | MS.gene43972 | 0.82115 | 4.70E-53 | -1.69E-46 |
| MS.gene052118 | MS.gene43972 | 0.818719 | 1.69E-52 | -1.69E-46 |
| MS.gene052239 | MS.gene43972 | -0.826158 | 3.16E-54 | -1.69E-46 |
| MS.gene052307 | MS.gene43972 | 0.812163 | 4.87E-51 | -1.69E-46 |
| MS.gene052463 | MS.gene43972 | 0.827325 | 1.66E-54 | -1.69E-46 |
| MS.gene052533 | MS.gene43972 | 0.811922 | 5.50E-51 | -1.69E-46 |
| MS.gene052609 | MS.gene43972 | 0.801845 | 7.43E-49 | -1.69E-46 |
| MS.gene052674 | MS.gene43972 | 0.815447 | 9.20E-52 | -1.69E-46 |
| MS.gene052675 | MS.gene43972 | 0.819109 | 1.38E-52 | -1.69E-46 |
| MS.gene052677 | MS.gene43972 | 0.823 | 1.75E-53 | -1.69E-46 |
| MS.gene053079 | MS.gene43972 | 0.809003 | 2.34E-50 | -1.69E-46 |
| MS.gene053272 | MS.gene43972 | -0.832488 | 9.18E-56 | -1.69E-46 |
| MS.gene053575 | MS.gene43972 | 0.818482 | 1.92E-52 | -1.69E-46 |
| MS.gene053931 | MS.gene43972 | 0.813442 | 2.55E-51 | -1.69E-46 |
| MS.gene053963 | MS.gene43972 | 0.810487 | 1.12E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene43972 | 0.801787 | 7.64E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene43972 | 0.804566 | 2.03E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene43972 | 0.818254 | 2.16E-52 | -1.69E-46 |
| MS.gene05454 | MS.gene43972 | 0.817553 | 3.11E-52 | -1.69E-46 |
| MS.gene054576 | MS.gene43972 | 0.817332 | 3.48E-52 | -1.69E-46 |
| MS.gene05458 | MS.gene43972 | 0.813142 | 2.97E-51 | -1.69E-46 |
| MS.gene054641 | MS.gene43972 | 0.813405 | 2.60E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene43972 | 0.835042 | 2.11E-56 | -1.69E-46 |
| MS.gene054892 | MS.gene43972 | 0.838588 | 2.63E-57 | -1.69E-46 |
| MS.gene054893 | MS.gene43972 | 0.838749 | 2.39E-57 | -1.69E-46 |
| MS.gene054957 | MS.gene43972 | 0.802152 | 6.42E-49 | -1.69E-46 |
| MS.gene055228 | MS.gene43972 | 0.807648 | 4.56E-50 | -1.69E-46 |
| MS.gene05539 | MS.gene43972 | 0.813313 | 2.73E-51 | -1.69E-46 |
| MS.gene055724 | MS.gene43972 | 0.833078 | 6.56E-56 | -1.69E-46 |
| MS.gene055926 | MS.gene43972 | 0.800475 | 1.42E-48 | -1.69E-46 |
| MS.gene056050 | MS.gene43972 | 0.810127 | 1.35E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene43972 | 0.81805 | 2.40E-52 | -1.69E-46 |
| MS.gene056363 | MS.gene43972 | 0.839366 | 1.65E-57 | -1.69E-46 |
| MS.gene056444 | MS.gene43972 | 0.823779 | 1.15E-53 | -1.69E-46 |
| MS.gene056519 | MS.gene43972 | 0.820991 | 5.12E-53 | -1.69E-46 |
| MS.gene056677 | MS.gene43972 | 0.800747 | 1.25E-48 | -1.69E-46 |
| MS.gene056699 | MS.gene43972 | 0.82416 | 9.38E-54 | -1.69E-46 |
| MS.gene05686 | MS.gene43972 | 0.909613 | 4.69E-82 | -1.69E-46 |
| MS.gene05690 | MS.gene43972 | 0.808828 | 2.56E-50 | -1.69E-46 |
| MS.gene056973 | MS.gene43972 | 0.841332 | 5.07E-58 | -1.69E-46 |
| MS.gene057102 | MS.gene43972 | 0.81334 | 2.69E-51 | -1.69E-46 |
| MS.gene057174 | MS.gene43972 | 0.814869 | 1.24E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene43972 | 0.852576 | 4.22E-61 | -1.69E-46 |
| MS.gene057598 | MS.gene43972 | 0.810055 | 1.39E-50 | -1.69E-46 |
| MS.gene057855 | MS.gene43972 | 0.806711 | 7.20E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene43972 | 0.807119 | 5.91E-50 | -1.69E-46 |
| MS.gene058009 | MS.gene43972 | 0.811626 | 6.37E-51 | -1.69E-46 |
| MS.gene058010 | MS.gene43972 | 0.824273 | 8.83E-54 | -1.69E-46 |
| MS.gene058159 | MS.gene43972 | 0.804642 | 1.96E-49 | -1.69E-46 |
| MS.gene058279 | MS.gene43972 | 0.818479 | 1.92E-52 | -1.69E-46 |
| MS.gene058289 | MS.gene43972 | 0.810482 | 1.13E-50 | -1.69E-46 |
| MS.gene058781 | MS.gene43972 | 0.848206 | 7.10E-60 | -1.69E-46 |
| MS.gene058783 | MS.gene43972 | 0.824794 | 6.65E-54 | -1.69E-46 |
| MS.gene058979 | MS.gene43972 | 0.84331 | 1.52E-58 | -1.69E-46 |
| MS.gene05952 | MS.gene43972 | 0.835684 | 1.45E-56 | -1.69E-46 |
| MS.gene059634 | MS.gene43972 | 0.836679 | 8.13E-57 | -1.69E-46 |
| MS.gene05985 | MS.gene43972 | -0.83557 | 1.55E-56 | -1.69E-46 |
| MS.gene060011 | MS.gene43972 | 0.801928 | 7.14E-49 | -1.69E-46 |
| MS.gene060373 | MS.gene43972 | 0.800016 | 1.76E-48 | -1.69E-46 |
| MS.gene060412 | MS.gene43972 | 0.808885 | 2.49E-50 | -1.69E-46 |
| MS.gene060435 | MS.gene43972 | 0.802566 | 5.28E-49 | -1.69E-46 |
| MS.gene060522 | MS.gene43972 | 0.827715 | 1.34E-54 | -1.69E-46 |
| MS.gene060525 | MS.gene43972 | 0.80772 | 4.40E-50 | -1.69E-46 |
| MS.gene060716 | MS.gene43972 | 0.800025 | 1.75E-48 | -1.69E-46 |
| MS.gene060777 | MS.gene43972 | 0.810149 | 1.33E-50 | -1.69E-46 |
| MS.gene060785 | MS.gene43972 | 0.819252 | 1.28E-52 | -1.69E-46 |
| MS.gene060840 | MS.gene43972 | 0.810118 | 1.35E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene43972 | 0.860079 | 2.65E-63 | -1.69E-46 |
| MS.gene060935 | MS.gene43972 | 0.871513 | 6.46E-67 | -1.69E-46 |
| MS.gene060937 | MS.gene43972 | 0.88043 | 5.53E-70 | -1.69E-46 |
| MS.gene061160 | MS.gene43972 | 0.803077 | 4.14E-49 | -1.69E-46 |
| MS.gene061188 | MS.gene43972 | 0.82168 | 3.55E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43972.t1 | MTR_1g080370 | 99.038 | 312 | 3 | 0 | 1 | 312 | 249 | 560 | 0.0 | 643 |
| MS.gene43972.t1 | MTR_1g080410 | 99.038 | 312 | 3 | 0 | 1 | 312 | 249 | 560 | 0.0 | 643 |
| MS.gene43972.t1 | MTR_5g007860 | 66.774 | 310 | 102 | 1 | 1 | 310 | 297 | 605 | 4.16e-157 | 452 |
| MS.gene43972.t1 | MTR_4g077620 | 63.871 | 310 | 110 | 2 | 1 | 310 | 260 | 567 | 1.85e-151 | 437 |
| MS.gene43972.t1 | MTR_2g062430 | 54.662 | 311 | 135 | 5 | 1 | 310 | 247 | 552 | 8.20e-118 | 350 |
| MS.gene43972.t1 | MTR_2g062370 | 52.038 | 319 | 140 | 5 | 1 | 310 | 275 | 589 | 3.26e-114 | 342 |
| MS.gene43972.t1 | MTR_2g067640 | 51.923 | 312 | 135 | 5 | 1 | 310 | 242 | 540 | 2.29e-111 | 333 |
| MS.gene43972.t1 | MTR_2g062410 | 51.746 | 315 | 143 | 5 | 1 | 310 | 275 | 585 | 3.66e-109 | 329 |
| MS.gene43972.t1 | MTR_2g062380 | 51.724 | 319 | 141 | 5 | 1 | 310 | 245 | 559 | 2.25e-107 | 323 |
| MS.gene43972.t1 | MTR_4g131710 | 50.467 | 321 | 144 | 6 | 1 | 310 | 269 | 585 | 1.49e-104 | 317 |
| MS.gene43972.t1 | MTR_2g062340 | 49.045 | 314 | 115 | 5 | 1 | 310 | 244 | 516 | 1.41e-95 | 292 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43972.t1 | AT3G04350 | 72.115 | 312 | 87 | 0 | 1 | 312 | 234 | 545 | 2.82e-177 | 501 |
| MS.gene43972.t1 | AT3G04350 | 72.115 | 312 | 87 | 0 | 1 | 312 | 256 | 567 | 1.42e-176 | 500 |
| MS.gene43972.t1 | AT5G18490 | 70.288 | 313 | 89 | 2 | 1 | 312 | 244 | 553 | 4.28e-167 | 476 |
| MS.gene43972.t1 | AT1G04090 | 63.057 | 314 | 114 | 2 | 1 | 312 | 259 | 572 | 1.36e-152 | 439 |
| MS.gene43972.t1 | AT5G43950 | 60.952 | 315 | 117 | 4 | 1 | 310 | 251 | 564 | 1.32e-139 | 406 |
| MS.gene43972.t1 | AT2G44260 | 50.479 | 313 | 149 | 4 | 1 | 310 | 243 | 552 | 3.96e-114 | 341 |
| MS.gene43972.t1 | AT2G44260 | 50.479 | 313 | 149 | 4 | 1 | 310 | 273 | 582 | 9.20e-114 | 341 |
| MS.gene43972.t1 | AT2G44230 | 49.841 | 315 | 148 | 6 | 1 | 310 | 232 | 541 | 2.57e-106 | 320 |
| MS.gene43972.t1 | AT3G01880 | 48.243 | 313 | 152 | 6 | 1 | 310 | 284 | 589 | 1.64e-97 | 299 |
| MS.gene43972.t1 | AT3G01870 | 47.436 | 312 | 155 | 5 | 1 | 310 | 279 | 583 | 1.09e-95 | 294 |
Find 84 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTCATCAATGACACTTTA+AGG | 0.107306 | 1.3:-56868373 | None:intergenic |
| CTGAGCACAGTGGAGGTAAA+TGG | 0.247800 | 1.3:+56868492 | MS.gene43972:CDS |
| TTATTTCCAACAGAGCTTTC+TGG | 0.254169 | 1.3:+56868866 | MS.gene43972:CDS |
| ATTCGATTTGGAGTTTATTA+AGG | 0.263278 | 1.3:+56868523 | MS.gene43972:CDS |
| AGCCAATTGTCCTTCTCCTT+TGG | 0.307063 | 1.3:-56868908 | None:intergenic |
| GCGAACTCCTATTCCAAGTT+TGG | 0.314359 | 1.3:-56868624 | None:intergenic |
| TGTCTTGATGAATAAACTAT+TGG | 0.322222 | 1.3:-56868554 | None:intergenic |
| ACAGAAAACCTAACAAATAT+TGG | 0.329471 | 1.3:-56868827 | None:intergenic |
| GATATAATGATGGTGCGTTT+TGG | 0.329648 | 1.3:+56868198 | MS.gene43972:CDS |
| ATGGGTTAATGCATTCGATT+TGG | 0.345620 | 1.3:+56868511 | MS.gene43972:CDS |
| TCCTTCTCCTTTGGACCAGT+TGG | 0.349557 | 1.3:-56868899 | None:intergenic |
| ACATGCTTCCAATATTTGTT+AGG | 0.353415 | 1.3:+56868819 | MS.gene43972:CDS |
| CATGCTCTTATTGAGCATTA+CGG | 0.358178 | 1.3:+56868032 | MS.gene43972:CDS |
| AATGACACTTTAAGGGTTGC+AGG | 0.360391 | 1.3:-56868365 | None:intergenic |
| ATGCTCTTATTGAGCATTAC+GGG | 0.370883 | 1.3:+56868033 | MS.gene43972:CDS |
| GTTCATCAATGACACTTTAA+GGG | 0.377575 | 1.3:-56868372 | None:intergenic |
| TCTGGTGAGGAGGGGCCAAC+TGG | 0.379305 | 1.3:+56868884 | MS.gene43972:CDS |
| CTGTTGGAAATAATTCAAAA+AGG | 0.390774 | 1.3:-56868856 | None:intergenic |
| TCATGCAGGGACTTACCTTC+AGG | 0.415789 | 1.3:+56868595 | MS.gene43972:CDS |
| AGGGTTGCAGGTCCATTGAA+TGG | 0.426014 | 1.3:-56868353 | None:intergenic |
| GTTCATGTAAAACCAGCATT+AGG | 0.426763 | 1.3:+56868293 | MS.gene43972:CDS |
| GCTCAATAAGAGCATGAATC+TGG | 0.427486 | 1.3:-56868025 | None:intergenic |
| CTTCAGGGATCATCCAAACT+TGG | 0.432488 | 1.3:+56868611 | MS.gene43972:CDS |
| CGGTGAACATGTCGGTGATT+GGG | 0.432674 | 1.3:+56868412 | MS.gene43972:CDS |
| TCGGTGAACATGTCGGTGAT+TGG | 0.434338 | 1.3:+56868411 | MS.gene43972:CDS |
| GCAACTTCACCGGAGAGCTT+TGG | 0.443367 | 1.3:+56868456 | MS.gene43972:CDS |
| GGATCATCCAAACTTGGAAT+AGG | 0.450282 | 1.3:+56868617 | MS.gene43972:CDS |
| GATGGCATGTATTTCTCATC+AGG | 0.457793 | 1.3:-56868074 | None:intergenic |
| TCCTCACCAGAAAGCTCTGT+TGG | 0.461764 | 1.3:-56868872 | None:intergenic |
| GCATGAATCTGGTTGAGATT+CGG | 0.468325 | 1.3:-56868014 | None:intergenic |
| TGAGCACAGTGGAGGTAAAT+GGG | 0.475593 | 1.3:+56868493 | MS.gene43972:CDS |
| CTTTACTGATATTGCAATGT+GGG | 0.475927 | 1.3:+56868322 | MS.gene43972:CDS |
| TGCGAGAAATAATCATAAGA+AGG | 0.480833 | 1.3:+56868244 | MS.gene43972:CDS |
| ATTGTTGCTGCCGAGTATCT+TGG | 0.490221 | 1.3:+56868698 | MS.gene43972:CDS |
| GTCCAAAGGAGAAGGACAAT+TGG | 0.490950 | 1.3:+56868906 | MS.gene43972:CDS |
| GGGTTGCAGGTCCATTGAAT+GGG | 0.500640 | 1.3:-56868352 | None:intergenic |
| CGGTGAAGTTGCTCACGCGA+AGG | 0.508371 | 1.3:-56868445 | None:intergenic |
| CATGCTAGCTATCCTCATGC+AGG | 0.510869 | 1.3:+56868581 | MS.gene43972:CDS |
| CCACATTGCAATATCAGTAA+AGG | 0.513990 | 1.3:-56868321 | None:intergenic |
| CATGCAGGGACTTACCTTCA+GGG | 0.530671 | 1.3:+56868596 | MS.gene43972:CDS |
| AACAGAGCTTTCTGGTGAGG+AGG | 0.540070 | 1.3:+56868874 | MS.gene43972:CDS |
| GTATACTGCGGGTAATGCAA+AGG | 0.543009 | 1.3:+56868133 | MS.gene43972:CDS |
| ACAGAGCTTTCTGGTGAGGA+GGG | 0.543440 | 1.3:+56868875 | MS.gene43972:CDS |
| GTAAAGGCTCCTCCTAATGC+TGG | 0.544725 | 1.3:-56868305 | None:intergenic |
| TTTCTCGCATCTTCGTTGGT+AGG | 0.548987 | 1.3:-56868230 | None:intergenic |
| ATTATTTCTCGCATCTTCGT+TGG | 0.555870 | 1.3:-56868234 | None:intergenic |
| GACTCCATCACCAAGATACT+CGG | 0.556847 | 1.3:-56868708 | None:intergenic |
| GGTGAAGTTGCTCACGCGAA+GGG | 0.560176 | 1.3:-56868444 | None:intergenic |
| CGTGAATCATATACAATGGT+AGG | 0.564534 | 1.3:-56868773 | None:intergenic |
| TCCAACAGAGCTTTCTGGTG+AGG | 0.565118 | 1.3:+56868871 | MS.gene43972:CDS |
| GCGAGAAATAATCATAAGAA+GGG | 0.567421 | 1.3:+56868245 | MS.gene43972:CDS |
| TATACTGCGGGTAATGCAAA+GGG | 0.571900 | 1.3:+56868134 | MS.gene43972:CDS |
| CATGTAAAACCAGCATTAGG+AGG | 0.572269 | 1.3:+56868296 | MS.gene43972:CDS |
| AACATTGAGATGAACAAGAT+CGG | 0.572272 | 1.3:+56868392 | MS.gene43972:CDS |
| CTTCGCGTGAGCAACTTCAC+CGG | 0.582952 | 1.3:+56868446 | MS.gene43972:CDS |
| GCCAACTGGTCCAAAGGAGA+AGG | 0.588714 | 1.3:+56868898 | MS.gene43972:CDS |
| ACATGCCATCATCGGTGTCG+TGG | 0.595421 | 1.3:+56868087 | MS.gene43972:CDS |
| TATTGAGCATTACGGGCCAA+CGG | 0.595833 | 1.3:+56868040 | MS.gene43972:CDS |
| CTCATATATTGCAACCAACA+TGG | 0.598495 | 1.3:-56868740 | None:intergenic |
| GGCACAAATTTACCTGGTGG+AGG | 0.603301 | 1.3:+56868176 | MS.gene43972:CDS |
| GGTTGCAATATATGAGAGAG+TGG | 0.604682 | 1.3:+56868747 | MS.gene43972:CDS |
| TGAGAAATACATGCCATCAT+CGG | 0.607689 | 1.3:+56868079 | MS.gene43972:CDS |
| TATCACGGCACAAATTTACC+TGG | 0.610377 | 1.3:+56868170 | MS.gene43972:CDS |
| AAGGAGAAGGACAATTGGCT+AGG | 0.611868 | 1.3:+56868911 | MS.gene43972:CDS |
| TAGTTTATTCATCAAGACAT+GGG | 0.614112 | 1.3:+56868558 | MS.gene43972:CDS |
| GGAGGGGCCAACTGGTCCAA+AGG | 0.615580 | 1.3:+56868892 | MS.gene43972:CDS |
| CCTGGTGGAGGATATAATGA+TGG | 0.615594 | 1.3:+56868188 | MS.gene43972:CDS |
| TCAGGATGAAAATATACCGT+TGG | 0.617396 | 1.3:-56868056 | None:intergenic |
| CAAGTTTGGATGATCCCTGA+AGG | 0.618933 | 1.3:-56868610 | None:intergenic |
| GGTAAAGCTATAGATTATCA+CGG | 0.619614 | 1.3:+56868155 | MS.gene43972:CDS |
| CCTTTACTGATATTGCAATG+TGG | 0.624376 | 1.3:+56868321 | MS.gene43972:CDS |
| CACGGCACAAATTTACCTGG+TGG | 0.625316 | 1.3:+56868173 | MS.gene43972:CDS |
| CCATCATTATATCCTCCACC+AGG | 0.627153 | 1.3:-56868188 | None:intergenic |
| GTTGCAATATATGAGAGAGT+GGG | 0.627285 | 1.3:+56868748 | MS.gene43972:CDS |
| GCTGCCGAGTATCTTGGTGA+TGG | 0.635810 | 1.3:+56868704 | MS.gene43972:CDS |
| GAAGGGAAACATAGAGAGTG+CGG | 0.636222 | 1.3:+56868262 | MS.gene43972:CDS |
| TGAAGGTAAGTCCCTGCATG+AGG | 0.636865 | 1.3:-56868593 | None:intergenic |
| ATAGTTTATTCATCAAGACA+TGG | 0.637290 | 1.3:+56868557 | MS.gene43972:CDS |
| AACAAGATCGGTGAACATGT+CGG | 0.653405 | 1.3:+56868404 | MS.gene43972:CDS |
| AGAACGTGAATCATATACAA+TGG | 0.654517 | 1.3:-56868777 | None:intergenic |
| ATGCTAGCTATCCTCATGCA+GGG | 0.655682 | 1.3:+56868582 | MS.gene43972:CDS |
| CAGAGCTTTCTGGTGAGGAG+GGG | 0.657357 | 1.3:+56868876 | MS.gene43972:CDS |
| GAGTCATTACAGAACCATGT+TGG | 0.666649 | 1.3:+56868726 | MS.gene43972:CDS |
| TTGCAATATATGAGAGAGTG+GGG | 0.676061 | 1.3:+56868749 | MS.gene43972:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGCTAAAAGTAATTTTATTC+TGG | + | chr1.3:56868655-56868674 | MS.gene43972:CDS | 20.0% |
| ! | ACAGAAAACCTAACAAATAT+TGG | - | chr1.3:56868830-56868849 | None:intergenic | 25.0% |
| ! | ATAGTTTATTCATCAAGACA+TGG | + | chr1.3:56868557-56868576 | MS.gene43972:CDS | 25.0% |
| ! | CTGTTGGAAATAATTCAAAA+AGG | - | chr1.3:56868859-56868878 | None:intergenic | 25.0% |
| ! | TAGTTTATTCATCAAGACAT+GGG | + | chr1.3:56868558-56868577 | MS.gene43972:CDS | 25.0% |
| ! | TGTCTTGATGAATAAACTAT+TGG | - | chr1.3:56868557-56868576 | None:intergenic | 25.0% |
| !!! | AATATTTGTTAGGTTTTCTG+TGG | + | chr1.3:56868829-56868848 | MS.gene43972:CDS | 25.0% |
| !!! | ATTCGATTTGGAGTTTATTA+AGG | + | chr1.3:56868523-56868542 | MS.gene43972:CDS | 25.0% |
| AACATTGAGATGAACAAGAT+CGG | + | chr1.3:56868392-56868411 | MS.gene43972:CDS | 30.0% | |
| ACATGCTTCCAATATTTGTT+AGG | + | chr1.3:56868819-56868838 | MS.gene43972:CDS | 30.0% | |
| AGAACGTGAATCATATACAA+TGG | - | chr1.3:56868780-56868799 | None:intergenic | 30.0% | |
| CTTTACTGATATTGCAATGT+GGG | + | chr1.3:56868322-56868341 | MS.gene43972:CDS | 30.0% | |
| GCGAGAAATAATCATAAGAA+GGG | + | chr1.3:56868245-56868264 | MS.gene43972:CDS | 30.0% | |
| GGTAAAGCTATAGATTATCA+CGG | + | chr1.3:56868155-56868174 | MS.gene43972:CDS | 30.0% | |
| GTTCATCAATGACACTTTAA+GGG | - | chr1.3:56868375-56868394 | None:intergenic | 30.0% | |
| TGCGAGAAATAATCATAAGA+AGG | + | chr1.3:56868244-56868263 | MS.gene43972:CDS | 30.0% | |
| TGTTCATCAATGACACTTTA+AGG | - | chr1.3:56868376-56868395 | None:intergenic | 30.0% | |
| ATGCTCTTATTGAGCATTAC+GGG | + | chr1.3:56868033-56868052 | MS.gene43972:CDS | 35.0% | |
| CATGCTCTTATTGAGCATTA+CGG | + | chr1.3:56868032-56868051 | MS.gene43972:CDS | 35.0% | |
| CCACATTGCAATATCAGTAA+AGG | - | chr1.3:56868324-56868343 | None:intergenic | 35.0% | |
| CCTTTACTGATATTGCAATG+TGG | + | chr1.3:56868321-56868340 | MS.gene43972:CDS | 35.0% | |
| CGTGAATCATATACAATGGT+AGG | - | chr1.3:56868776-56868795 | None:intergenic | 35.0% | |
| CTCATATATTGCAACCAACA+TGG | - | chr1.3:56868743-56868762 | None:intergenic | 35.0% | |
| GTTCATGTAAAACCAGCATT+AGG | + | chr1.3:56868293-56868312 | MS.gene43972:CDS | 35.0% | |
| GTTGCAATATATGAGAGAGT+GGG | + | chr1.3:56868748-56868767 | MS.gene43972:CDS | 35.0% | |
| TCAGGATGAAAATATACCGT+TGG | - | chr1.3:56868059-56868078 | None:intergenic | 35.0% | |
| TGAGAAATACATGCCATCAT+CGG | + | chr1.3:56868079-56868098 | MS.gene43972:CDS | 35.0% | |
| TTATTTCCAACAGAGCTTTC+TGG | + | chr1.3:56868866-56868885 | MS.gene43972:CDS | 35.0% | |
| TTGCAATATATGAGAGAGTG+GGG | + | chr1.3:56868749-56868768 | MS.gene43972:CDS | 35.0% | |
| ! | ATGGGTTAATGCATTCGATT+TGG | + | chr1.3:56868511-56868530 | MS.gene43972:CDS | 35.0% |
| ! | ATTATTTCTCGCATCTTCGT+TGG | - | chr1.3:56868237-56868256 | None:intergenic | 35.0% |
| ! | TGGAGCAATTTTGTATACTG+CGG | + | chr1.3:56868121-56868140 | MS.gene43972:CDS | 35.0% |
| !! | GATATAATGATGGTGCGTTT+TGG | + | chr1.3:56868198-56868217 | MS.gene43972:CDS | 35.0% |
| AAAAACAGACCAAAGCTCTC+CGG | - | chr1.3:56868468-56868487 | None:intergenic | 40.0% | |
| AACAAGATCGGTGAACATGT+CGG | + | chr1.3:56868404-56868423 | MS.gene43972:CDS | 40.0% | |
| CATGTAAAACCAGCATTAGG+AGG | + | chr1.3:56868296-56868315 | MS.gene43972:CDS | 40.0% | |
| GAGTCATTACAGAACCATGT+TGG | + | chr1.3:56868726-56868745 | MS.gene43972:CDS | 40.0% | |
| GATGGCATGTATTTCTCATC+AGG | - | chr1.3:56868077-56868096 | None:intergenic | 40.0% | |
| GCATGAATCTGGTTGAGATT+CGG | - | chr1.3:56868017-56868036 | None:intergenic | 40.0% | |
| GGATCATCCAAACTTGGAAT+AGG | + | chr1.3:56868617-56868636 | MS.gene43972:CDS | 40.0% | |
| GGTTGCAATATATGAGAGAG+TGG | + | chr1.3:56868747-56868766 | MS.gene43972:CDS | 40.0% | |
| TATACTGCGGGTAATGCAAA+GGG | + | chr1.3:56868134-56868153 | MS.gene43972:CDS | 40.0% | |
| TATCACGGCACAAATTTACC+TGG | + | chr1.3:56868170-56868189 | MS.gene43972:CDS | 40.0% | |
| ! | AATGACACTTTAAGGGTTGC+AGG | - | chr1.3:56868368-56868387 | None:intergenic | 40.0% |
| ! | GCTCAATAAGAGCATGAATC+TGG | - | chr1.3:56868028-56868047 | None:intergenic | 40.0% |
| ! | GGAGCAATTTTGTATACTGC+GGG | + | chr1.3:56868122-56868141 | MS.gene43972:CDS | 40.0% |
| !!! | GTGTCGTGGTTTTTCAAGAA+TGG | + | chr1.3:56868101-56868120 | MS.gene43972:CDS | 40.0% |
| !!! | GTTTTTTTCTCTGAGCACAG+TGG | + | chr1.3:56868482-56868501 | MS.gene43972:CDS | 40.0% |
| !!! | TGGGTTTTTTGCCCATTCAA+TGG | + | chr1.3:56868341-56868360 | MS.gene43972:CDS | 40.0% |
| AAAAACCACGACACCGATGA+TGG | - | chr1.3:56868095-56868114 | None:intergenic | 45.0% | |
| AAGGAGAAGGACAATTGGCT+AGG | + | chr1.3:56868911-56868930 | MS.gene43972:CDS | 45.0% | |
| AGCCAATTGTCCTTCTCCTT+TGG | - | chr1.3:56868911-56868930 | None:intergenic | 45.0% | |
| ATGCTAGCTATCCTCATGCA+GGG | + | chr1.3:56868582-56868601 | MS.gene43972:CDS | 45.0% | |
| ATTGTTGCTGCCGAGTATCT+TGG | + | chr1.3:56868698-56868717 | MS.gene43972:CDS | 45.0% | |
| CAAGTTTGGATGATCCCTGA+AGG | - | chr1.3:56868613-56868632 | None:intergenic | 45.0% | |
| CCATCATTATATCCTCCACC+AGG | - | chr1.3:56868191-56868210 | None:intergenic | 45.0% | |
| CCTGGTGGAGGATATAATGA+TGG | + | chr1.3:56868188-56868207 | MS.gene43972:CDS | 45.0% | |
| CTTCAGGGATCATCCAAACT+TGG | + | chr1.3:56868611-56868630 | MS.gene43972:CDS | 45.0% | |
| GAAGGGAAACATAGAGAGTG+CGG | + | chr1.3:56868262-56868281 | MS.gene43972:CDS | 45.0% | |
| GACTCCATCACCAAGATACT+CGG | - | chr1.3:56868711-56868730 | None:intergenic | 45.0% | |
| GCGAACTCCTATTCCAAGTT+TGG | - | chr1.3:56868627-56868646 | None:intergenic | 45.0% | |
| GTATACTGCGGGTAATGCAA+AGG | + | chr1.3:56868133-56868152 | MS.gene43972:CDS | 45.0% | |
| GTCCAAAGGAGAAGGACAAT+TGG | + | chr1.3:56868906-56868925 | MS.gene43972:CDS | 45.0% | |
| TATTGAGCATTACGGGCCAA+CGG | + | chr1.3:56868040-56868059 | MS.gene43972:CDS | 45.0% | |
| TGAGCACAGTGGAGGTAAAT+GGG | + | chr1.3:56868493-56868512 | MS.gene43972:CDS | 45.0% | |
| TTTCTCGCATCTTCGTTGGT+AGG | - | chr1.3:56868233-56868252 | None:intergenic | 45.0% | |
| ! | TTTTTCTCTGAGCACAGTGG+AGG | + | chr1.3:56868485-56868504 | MS.gene43972:CDS | 45.0% |
| AGGGTTGCAGGTCCATTGAA+TGG | - | chr1.3:56868356-56868375 | None:intergenic | 50.0% | |
| CACGGCACAAATTTACCTGG+TGG | + | chr1.3:56868173-56868192 | MS.gene43972:CDS | 50.0% | |
| CATGCAGGGACTTACCTTCA+GGG | + | chr1.3:56868596-56868615 | MS.gene43972:CDS | 50.0% | |
| CATGCTAGCTATCCTCATGC+AGG | + | chr1.3:56868581-56868600 | MS.gene43972:CDS | 50.0% | |
| CGGTGAACATGTCGGTGATT+GGG | + | chr1.3:56868412-56868431 | MS.gene43972:CDS | 50.0% | |
| CTGAGCACAGTGGAGGTAAA+TGG | + | chr1.3:56868492-56868511 | MS.gene43972:CDS | 50.0% | |
| GGCACAAATTTACCTGGTGG+AGG | + | chr1.3:56868176-56868195 | MS.gene43972:CDS | 50.0% | |
| GGGTTGCAGGTCCATTGAAT+GGG | - | chr1.3:56868355-56868374 | None:intergenic | 50.0% | |
| GTAAAGGCTCCTCCTAATGC+TGG | - | chr1.3:56868308-56868327 | None:intergenic | 50.0% | |
| TCATGCAGGGACTTACCTTC+AGG | + | chr1.3:56868595-56868614 | MS.gene43972:CDS | 50.0% | |
| TCCTCACCAGAAAGCTCTGT+TGG | - | chr1.3:56868875-56868894 | None:intergenic | 50.0% | |
| TCCTTCTCCTTTGGACCAGT+TGG | - | chr1.3:56868902-56868921 | None:intergenic | 50.0% | |
| TCGGTGAACATGTCGGTGAT+TGG | + | chr1.3:56868411-56868430 | MS.gene43972:CDS | 50.0% | |
| TGAAGGTAAGTCCCTGCATG+AGG | - | chr1.3:56868596-56868615 | None:intergenic | 50.0% | |
| ! | AACAGAGCTTTCTGGTGAGG+AGG | + | chr1.3:56868874-56868893 | MS.gene43972:CDS | 50.0% |
| ! | ACAGAGCTTTCTGGTGAGGA+GGG | + | chr1.3:56868875-56868894 | MS.gene43972:CDS | 50.0% |
| ! | TCCAACAGAGCTTTCTGGTG+AGG | + | chr1.3:56868871-56868890 | MS.gene43972:CDS | 50.0% |
| CTTCGCGTGAGCAACTTCAC+CGG | + | chr1.3:56868446-56868465 | MS.gene43972:CDS | 55.0% | |
| GCAACTTCACCGGAGAGCTT+TGG | + | chr1.3:56868456-56868475 | MS.gene43972:CDS | 55.0% | |
| GCCAACTGGTCCAAAGGAGA+AGG | + | chr1.3:56868898-56868917 | MS.gene43972:CDS | 55.0% | |
| GCTGCCGAGTATCTTGGTGA+TGG | + | chr1.3:56868704-56868723 | MS.gene43972:CDS | 55.0% | |
| GGTGAAGTTGCTCACGCGAA+GGG | - | chr1.3:56868447-56868466 | None:intergenic | 55.0% | |
| ! | CAGAGCTTTCTGGTGAGGAG+GGG | + | chr1.3:56868876-56868895 | MS.gene43972:CDS | 55.0% |
| !! | ACATGCCATCATCGGTGTCG+TGG | + | chr1.3:56868087-56868106 | MS.gene43972:CDS | 55.0% |
| CGGTGAAGTTGCTCACGCGA+AGG | - | chr1.3:56868448-56868467 | None:intergenic | 60.0% | |
| GGAGGGGCCAACTGGTCCAA+AGG | + | chr1.3:56868892-56868911 | MS.gene43972:CDS | 65.0% | |
| TCTGGTGAGGAGGGGCCAAC+TGG | + | chr1.3:56868884-56868903 | MS.gene43972:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 56868011 | 56868949 | 56868011 | ID=MS.gene43972 |
| chr1.3 | mRNA | 56868011 | 56868949 | 56868011 | ID=MS.gene43972.t1;Parent=MS.gene43972 |
| chr1.3 | exon | 56868011 | 56868949 | 56868011 | ID=MS.gene43972.t1.exon1;Parent=MS.gene43972.t1 |
| chr1.3 | CDS | 56868011 | 56868949 | 56868011 | ID=cds.MS.gene43972.t1;Parent=MS.gene43972.t1 |
| Gene Sequence |
| Protein sequence |
