AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08862


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08862.t1 MTR_8g097140 84.322 236 33 2 1 233 1 235 1.96e-133 375
MS.gene08862.t1 MTR_1g016270 47.909 263 85 7 3 218 4 261 8.30e-57 182
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAAAATTGGAGAAAATTT+TGG 0.213621 4.2:-13739214 None:intergenic
GGAGGCTATGATGTTATTCT+TGG 0.292173 4.2:+13739258 MS.gene08862:CDS
TCTGCTGACAATGGTGCTTC+AGG 0.313597 4.2:+13739237 MS.gene08862:CDS
AAAGAGTTTCGCAATGTTGA+TGG 0.333069 4.2:+13739402 MS.gene08862:CDS
GATTCACAACGACCGGTAAT+CGG 0.388566 4.2:-13739162 None:intergenic
CGAAATTCGATGCCGATTAC+CGG 0.390204 4.2:+13739150 None:intergenic
TGGAGGGCCGATGGGTCCTC+GGG 0.410669 4.2:+13739695 MS.gene08862:CDS
GTTTAAGTTCCTTGTGAAGC+TGG 0.424424 4.2:-13739311 None:intergenic
GTCCTCGGGACATAGAAATT+AGG 0.434748 4.2:+13739709 MS.gene08862:CDS
AAATTCAATCAAGAAGTTAG+TGG 0.438478 4.2:+13739447 MS.gene08862:CDS
GTGGAGGGCCGATGGGTCCT+CGG 0.438544 4.2:+13739694 MS.gene08862:CDS
AACTTTATGCTGCGGAGAAA+AGG 0.440522 4.2:+13739766 MS.gene08862:CDS
ATTGTCAGCAGAGACAAAAT+TGG 0.442188 4.2:-13739226 None:intergenic
TTAAACTATCAAAATCTCAT+AGG 0.445350 4.2:+13739328 MS.gene08862:CDS
GAGAGAATTGAAAGTATGTC+GGG 0.447028 4.2:+13739807 MS.gene08862:CDS
CGGAGGGTTAGTTGCTCCTC+GGG 0.449386 4.2:+13739527 MS.gene08862:CDS
TATTCTTGGAAAGACTTTCA+AGG 0.486432 4.2:+13739272 MS.gene08862:CDS
AGAGAGAATTGAAAGTATGT+CGG 0.498267 4.2:+13739806 MS.gene08862:CDS
ACAAACAAGAAACTTGAAGT+AGG 0.507231 4.2:+13739732 MS.gene08862:CDS
TTTAGATGATTCACAACGAC+CGG 0.509528 4.2:-13739169 None:intergenic
TGCGAAGAGTGGAGGGCCGA+TGG 0.514612 4.2:+13739686 MS.gene08862:CDS
ATTCTTGGAAAGACTTTCAA+GGG 0.523914 4.2:+13739273 MS.gene08862:CDS
GGGACAAGAACAACAGAAAG+AGG 0.527695 4.2:+13739641 MS.gene08862:CDS
GCGAAGAGTGGAGGGCCGAT+GGG 0.536215 4.2:+13739687 MS.gene08862:CDS
CTCCTCGGGATGTAGAAGTC+AGG 0.538589 4.2:+13739541 MS.gene08862:CDS
TTTAGAAGTTGAAAATGCGA+AGG 0.547721 4.2:+13739620 MS.gene08862:CDS
GCGGAGGGTTAGTTGCTCCT+CGG 0.558909 4.2:+13739526 MS.gene08862:CDS
CAAACAAGAAACTTGAAGTA+GGG 0.559654 4.2:+13739733 MS.gene08862:CDS
AGCAATGATGCGAAGAGTGG+AGG 0.563241 4.2:+13739678 MS.gene08862:CDS
GCAATGATGCGAAGAGTGGA+GGG 0.571141 4.2:+13739679 MS.gene08862:CDS
GAATGAAGCCGTGAGTGAGA+AGG 0.573910 4.2:+13739575 MS.gene08862:CDS
TAAGAAGAAAATGAAACACT+AGG 0.578476 4.2:+13739839 MS.gene08862:CDS
TGAGAAGGAGAAAATGCAAA+GGG 0.591298 4.2:+13739590 MS.gene08862:CDS
AAGAAGAAAATGAAACACTA+GGG 0.592449 4.2:+13739840 MS.gene08862:CDS
GTCCTAATTTCTATGTCCCG+AGG 0.595550 4.2:-13739711 None:intergenic
TTCTAAGCAACAGAAAGAGA+TGG 0.598515 4.2:+13739470 MS.gene08862:CDS
CTAAGCAATGATGCGAAGAG+TGG 0.602938 4.2:+13739675 MS.gene08862:CDS
CTTGCTCAACCAGCTTCACA+AGG 0.605167 4.2:+13739302 MS.gene08862:CDS
AAAGATAAGTAACAATGCGA+AGG 0.605305 4.2:+13739497 MS.gene08862:CDS
TCTATGTCCCGAGGACCCAT+CGG 0.610923 4.2:-13739702 None:intergenic
TTAGAAGTTGAAAATGCGAA+GGG 0.610992 4.2:+13739621 MS.gene08862:CDS
GCTGACAATGGTGCTTCAGG+AGG 0.622331 4.2:+13739240 MS.gene08862:CDS
GAGAAAAGGAAGAATCAAGA+TGG 0.623121 4.2:+13739780 MS.gene08862:CDS
GACCTGACTTCTACATCCCG+AGG 0.639086 4.2:-13739543 None:intergenic
GTGAGAAGGAGAAAATGCAA+AGG 0.641271 4.2:+13739589 MS.gene08862:CDS
AATGCGAAGGTTGAAAGCGG+AGG 0.641854 4.2:+13739510 MS.gene08862:CDS
ATGCGAAGGTTGAAAGCGGA+GGG 0.642455 4.2:+13739511 MS.gene08862:CDS
TGAGAGTAAACTTTATGCTG+CGG 0.645952 4.2:+13739758 MS.gene08862:CDS
AACAATGCGAAGGTTGAAAG+CGG 0.662379 4.2:+13739507 MS.gene08862:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAACTATCAAAATCTCAT+AGG + chr4.2:13739328-13739347 MS.gene08862:CDS 20.0%
! AAATTCAATCAAGAAGTTAG+TGG + chr4.2:13739447-13739466 MS.gene08862:CDS 25.0%
!! GACAAAATTGGAGAAAATTT+TGG - chr4.2:13739217-13739236 None:intergenic 25.0%
AAAGATAAGTAACAATGCGA+AGG + chr4.2:13739497-13739516 MS.gene08862:CDS 30.0%
ACAAACAAGAAACTTGAAGT+AGG + chr4.2:13739732-13739751 MS.gene08862:CDS 30.0%
ATTCTTGGAAAGACTTTCAA+GGG + chr4.2:13739273-13739292 MS.gene08862:CDS 30.0%
CAAACAAGAAACTTGAAGTA+GGG + chr4.2:13739733-13739752 MS.gene08862:CDS 30.0%
TATTCTTGGAAAGACTTTCA+AGG + chr4.2:13739272-13739291 MS.gene08862:CDS 30.0%
TTAGAAGTTGAAAATGCGAA+GGG + chr4.2:13739621-13739640 MS.gene08862:CDS 30.0%
TTTAGAAGTTGAAAATGCGA+AGG + chr4.2:13739620-13739639 MS.gene08862:CDS 30.0%
! AGAGAGAATTGAAAGTATGT+CGG + chr4.2:13739806-13739825 MS.gene08862:CDS 30.0%
AAAGAGTTTCGCAATGTTGA+TGG + chr4.2:13739402-13739421 MS.gene08862:CDS 35.0%
ATTGTCAGCAGAGACAAAAT+TGG - chr4.2:13739229-13739248 None:intergenic 35.0%
GAGAAAAGGAAGAATCAAGA+TGG + chr4.2:13739780-13739799 MS.gene08862:CDS 35.0%
TAGAAGTCAGGTCAAAAAAG+AGG + chr4.2:13739553-13739572 MS.gene08862:CDS 35.0%
TGAGAAGGAGAAAATGCAAA+GGG + chr4.2:13739590-13739609 MS.gene08862:CDS 35.0%
TGAGAGTAAACTTTATGCTG+CGG + chr4.2:13739758-13739777 MS.gene08862:CDS 35.0%
TTCTAAGCAACAGAAAGAGA+TGG + chr4.2:13739470-13739489 MS.gene08862:CDS 35.0%
TTTAGATGATTCACAACGAC+CGG - chr4.2:13739172-13739191 None:intergenic 35.0%
! AATTTTGTCTCTGCTGACAA+TGG + chr4.2:13739228-13739247 MS.gene08862:CDS 35.0%
! GAGAGAATTGAAAGTATGTC+GGG + chr4.2:13739807-13739826 MS.gene08862:CDS 35.0%
AACAATGCGAAGGTTGAAAG+CGG + chr4.2:13739507-13739526 MS.gene08862:CDS 40.0%
AACTTTATGCTGCGGAGAAA+AGG + chr4.2:13739766-13739785 MS.gene08862:CDS 40.0%
GGAGGCTATGATGTTATTCT+TGG + chr4.2:13739258-13739277 MS.gene08862:CDS 40.0%
GTGAGAAGGAGAAAATGCAA+AGG + chr4.2:13739589-13739608 MS.gene08862:CDS 40.0%
! CATTTTCTCCTTCTCACTCA+CGG - chr4.2:13739586-13739605 None:intergenic 40.0%
! GTTTAAGTTCCTTGTGAAGC+TGG - chr4.2:13739314-13739333 None:intergenic 40.0%
CTAAGCAATGATGCGAAGAG+TGG + chr4.2:13739675-13739694 MS.gene08862:CDS 45.0%
GATTCACAACGACCGGTAAT+CGG - chr4.2:13739165-13739184 None:intergenic 45.0%
GGGACAAGAACAACAGAAAG+AGG + chr4.2:13739641-13739660 MS.gene08862:CDS 45.0%
GTCCTAATTTCTATGTCCCG+AGG - chr4.2:13739714-13739733 None:intergenic 45.0%
GTCCTCGGGACATAGAAATT+AGG + chr4.2:13739709-13739728 MS.gene08862:CDS 45.0%
!! GGAGAAAATTTTGGCAGCTC+TGG - chr4.2:13739208-13739227 None:intergenic 45.0%
AATGCGAAGGTTGAAAGCGG+AGG + chr4.2:13739510-13739529 MS.gene08862:CDS 50.0%
AGCAATGATGCGAAGAGTGG+AGG + chr4.2:13739678-13739697 MS.gene08862:CDS 50.0%
ATGCGAAGGTTGAAAGCGGA+GGG + chr4.2:13739511-13739530 MS.gene08862:CDS 50.0%
CTTGCTCAACCAGCTTCACA+AGG + chr4.2:13739302-13739321 MS.gene08862:CDS 50.0%
GAATGAAGCCGTGAGTGAGA+AGG + chr4.2:13739575-13739594 MS.gene08862:CDS 50.0%
GCAATGATGCGAAGAGTGGA+GGG + chr4.2:13739679-13739698 MS.gene08862:CDS 50.0%
TCTGCTGACAATGGTGCTTC+AGG + chr4.2:13739237-13739256 MS.gene08862:CDS 50.0%
CTCCTCGGGATGTAGAAGTC+AGG + chr4.2:13739541-13739560 MS.gene08862:CDS 55.0%
GACCTGACTTCTACATCCCG+AGG - chr4.2:13739546-13739565 None:intergenic 55.0%
GCTGACAATGGTGCTTCAGG+AGG + chr4.2:13739240-13739259 MS.gene08862:CDS 55.0%
TCTATGTCCCGAGGACCCAT+CGG - chr4.2:13739705-13739724 None:intergenic 55.0%
!! CGGAGGGTTAGTTGCTCCTC+GGG + chr4.2:13739527-13739546 MS.gene08862:CDS 60.0%
!! GCGGAGGGTTAGTTGCTCCT+CGG + chr4.2:13739526-13739545 MS.gene08862:CDS 60.0%
GCGAAGAGTGGAGGGCCGAT+GGG + chr4.2:13739687-13739706 MS.gene08862:CDS 65.0%
TGCGAAGAGTGGAGGGCCGA+TGG + chr4.2:13739686-13739705 MS.gene08862:CDS 65.0%
GTGGAGGGCCGATGGGTCCT+CGG + chr4.2:13739694-13739713 MS.gene08862:CDS 70.0%
TGGAGGGCCGATGGGTCCTC+GGG + chr4.2:13739695-13739714 MS.gene08862:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 13739159 13739860 13739159 ID=MS.gene08862
chr4.2 mRNA 13739159 13739860 13739159 ID=MS.gene08862.t1;Parent=MS.gene08862
chr4.2 exon 13739159 13739860 13739159 ID=MS.gene08862.t1.exon1;Parent=MS.gene08862.t1
chr4.2 CDS 13739159 13739860 13739159 ID=cds.MS.gene08862.t1;Parent=MS.gene08862.t1
Gene Sequence

>MS.gene08862

ATGCCGATTACCGGTCGTTGTGAATCATCTAAAGAGATTTCGCTTTCCAGAGCTGCCAAAATTTTCTCCAATTTTGTCTCTGCTGACAATGGTGCTTCAGGAGGCTATGATGTTATTCTTGGAAAGACTTTCAAGGGTTTCAACTTGCTCAACCAGCTTCACAAGGAACTTAAACTATCAAAATCTCATAGGAAGAATCGTCAAAGCTACATAACAGAAGATAGTGTTGATGTGAAATCTGAAAAAGAGTTTCGCAATGTTGATGGAAAGTTGAATCAGAATGATGTTAAATTCAATCAAGAAGTTAGTGGTTCTAAGCAACAGAAAGAGATGGAGAAAAAGATAAGTAACAATGCGAAGGTTGAAAGCGGAGGGTTAGTTGCTCCTCGGGATGTAGAAGTCAGGTCAAAAAAGAGGAATGAAGCCGTGAGTGAGAAGGAGAAAATGCAAAGGGACAAGAATTTAGAAGTTGAAAATGCGAAGGGACAAGAACAACAGAAAGAGGTAGAGAAAAAACTAAGCAATGATGCGAAGAGTGGAGGGCCGATGGGTCCTCGGGACATAGAAATTAGGACAAACAAGAAACTTGAAGTAGGGAGTGAGAGTAAACTTTATGCTGCGGAGAAAAGGAAGAATCAAGATGGTGAAGAGAGAATTGAAAGTATGTCGGGAGAACATTCTAAGAAGAAAATGAAACACTAG

Protein sequence

>MS.gene08862.t1

MPITGRCESSKEISLSRAAKIFSNFVSADNGASGGYDVILGKTFKGFNLLNQLHKELKLSKSHRKNRQSYITEDSVDVKSEKEFRNVDGKLNQNDVKFNQEVSGSKQQKEMEKKISNNAKVESGGLVAPRDVEVRSKKRNEAVSEKEKMQRDKNLEVENAKGQEQQKEVEKKLSNDAKSGGPMGPRDIEIRTNKKLEVGSESKLYAAEKRKNQDGEERIESMSGEHSKKKMKH