AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065785

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065785.t1 MTR_4g079770 95.362 345 14 1 1 343 1 345 0.0 687
MS.gene065785.t1 MTR_4g079470 96.914 324 10 0 20 343 22 345 0.0 661
MS.gene065785.t1 MTR_5g022560 88.406 345 37 3 1 343 1 344 0.0 634
MS.gene065785.t1 MTR_4g079800 92.284 324 25 0 20 343 22 345 0.0 632
MS.gene065785.t1 MTR_4g107930 87.861 346 38 3 1 343 1 345 0.0 629
MS.gene065785.t1 MTR_4g047610 88.696 345 35 3 1 343 1 343 0.0 617
MS.gene065785.t1 MTR_2g075830 76.522 345 75 5 1 343 1 341 0.0 523
MS.gene065785.t1 MTR_4g080700 65.528 322 103 7 24 343 25 340 8.57e-152 430
MS.gene065785.t1 MTR_4g080730 65.217 322 104 6 24 343 25 340 5.21e-151 428
MS.gene065785.t1 MTR_4g080360 53.468 346 154 5 1 343 1 342 1.20e-130 377
MS.gene065785.t1 MTR_4g104370 57.227 339 134 9 9 341 9 342 3.59e-130 376
MS.gene065785.t1 MTR_2g083930 52.907 344 146 6 5 341 8 342 1.45e-127 369
MS.gene065785.t1 MTR_3g075390 53.846 338 146 7 9 341 11 343 3.08e-126 366
MS.gene065785.t1 MTR_4g079440 55.128 312 133 4 31 341 38 343 7.53e-122 354
MS.gene065785.t1 MTR_3g116080 52.493 341 147 7 10 342 14 347 6.77e-121 352
MS.gene065785.t1 MTR_1g107550 54.574 317 136 5 30 341 41 354 1.69e-118 350
MS.gene065785.t1 MTR_7g078600 57.419 310 120 7 34 341 37 336 3.02e-118 345
MS.gene065785.t1 MTR_2g090890 49.263 339 160 5 9 342 5 336 7.03e-117 342
MS.gene065785.t1 MTR_7g079230 52.212 339 149 8 8 341 4 334 1.41e-116 341
MS.gene065785.t1 MTR_4g094918 48.980 343 161 8 9 342 4 341 5.78e-114 335
MS.gene065785.t1 MTR_3g102220 48.229 367 157 10 1 341 1 360 2.81e-112 335
MS.gene065785.t1 MTR_1g018840 51.682 327 138 7 25 341 44 360 2.26e-111 332
MS.gene065785.t1 MTR_1g018840 51.524 328 139 7 24 341 43 360 3.27e-111 332
MS.gene065785.t1 MTR_3g112430 50.161 311 141 5 40 342 37 341 2.11e-102 308
MS.gene065785.t1 MTR_2g015850 54.828 290 123 6 55 341 78 362 3.31e-99 298
MS.gene065785.t1 MTR_2g016180 43.931 346 173 9 10 342 18 355 1.88e-89 276
MS.gene065785.t1 MTR_2g016270 44.444 342 162 10 10 342 18 340 9.66e-88 272
MS.gene065785.t1 MTR_2g067900 44.375 320 155 12 38 342 15 326 3.34e-77 240
MS.gene065785.t1 MTR_2g067560 44.548 321 146 13 38 337 15 324 1.75e-72 241
MS.gene065785.t1 MTR_4g125300 41.234 308 163 10 41 342 53 348 1.33e-69 221
MS.gene065785.t1 MTR_2g064475 39.429 350 154 16 38 342 15 351 7.53e-69 219
MS.gene065785.t1 MTR_2g015920 39.490 314 176 10 37 342 51 358 2.65e-67 216
MS.gene065785.t1 MTR_4g065077 37.963 324 183 9 29 342 41 356 7.71e-67 214
MS.gene065785.t1 MTR_2g066980 34.831 356 171 11 8 342 3 318 8.23e-67 213
MS.gene065785.t1 MTR_4g071060 37.006 354 184 12 10 334 5 348 1.26e-65 211
MS.gene065785.t1 MTR_7g078630 45.487 277 110 5 37 302 704 950 7.91e-65 221
MS.gene065785.t1 MTR_1g023210 38.602 329 160 12 31 334 41 352 7.99e-64 207
MS.gene065785.t1 MTR_8g086470 36.957 322 173 9 29 342 57 356 5.80e-62 202
MS.gene065785.t1 MTR_1g023810 45.022 231 118 6 113 340 37 261 1.59e-61 197
MS.gene065785.t1 MTR_2g015930 39.252 321 168 12 37 342 50 358 2.50e-61 200
MS.gene065785.t1 MTR_1g094135 36.499 337 177 12 22 334 35 358 3.30e-57 190
MS.gene065785.t1 MTR_4g072790 34.343 297 158 8 41 334 50 312 6.81e-45 156
MS.gene065785.t1 MTR_4g072340 34.219 301 157 10 41 334 50 316 6.05e-42 149
MS.gene065785.t1 MTR_4g073660 33.887 301 158 10 41 334 49 315 7.77e-42 149
MS.gene065785.t1 MTR_7g078580 64.286 98 34 1 244 341 4 100 1.47e-41 141
MS.gene065785.t1 MTR_7g079130 70.000 90 27 0 252 341 7 96 9.50e-41 138
MS.gene065785.t1 MTR_2g015840 49.367 158 74 5 6 160 2 156 1.18e-40 144
MS.gene065785.t1 MTR_2g015840 55.357 112 49 1 231 342 161 271 5.30e-36 132
MS.gene065785.t1 MTR_1g025450 38.503 187 90 7 113 295 43 208 1.52e-32 127
MS.gene065785.t1 MTR_7g078310 45.000 140 67 3 82 218 115 247 2.40e-27 108
MS.gene065785.t1 MTR_2g016380 47.107 121 57 4 226 342 18 135 4.62e-27 107
MS.gene065785.t1 MTR_7g079260 61.111 72 12 1 268 339 7 62 9.26e-23 91.7
MS.gene065785.t1 MTR_7g111060 28.889 225 132 7 131 334 113 330 1.60e-22 97.1
MS.gene065785.t1 MTR_7g111050 29.787 235 135 8 131 342 110 337 6.42e-22 95.5
MS.gene065785.t1 MTR_3g029560 27.888 251 146 9 98 342 16 237 5.67e-21 90.5
MS.gene065785.t1 MTR_8g087510 41.985 131 69 5 166 294 23 148 7.74e-21 88.6
MS.gene065785.t1 MTR_2g047960 48.235 85 43 1 134 218 7 90 7.85e-18 78.2
MS.gene065785.t1 MTR_3g082370 26.087 207 133 5 130 321 5 206 2.76e-17 80.1
MS.gene065785.t1 MTR_7g111050 27.692 195 117 6 131 304 105 296 1.10e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065785.t1 AT5G45890 54.942 344 146 4 6 342 4 345 3.87e-136 391
MS.gene065785.t1 AT5G50260 54.624 346 133 8 11 341 5 341 3.09e-130 376
MS.gene065785.t1 AT3G48340 52.907 344 144 7 11 341 4 342 6.95e-123 358
MS.gene065785.t1 AT2G34080 50.877 342 157 6 9 343 8 345 1.65e-120 351
MS.gene065785.t1 AT4G35350 51.312 343 151 8 9 342 17 352 5.10e-118 345
MS.gene065785.t1 AT3G48350 51.297 347 152 8 9 342 1 343 1.62e-116 342
MS.gene065785.t1 AT3G19390 50.000 350 159 7 3 342 2 345 2.20e-116 344
MS.gene065785.t1 AT5G43060 52.761 326 140 6 23 341 34 352 4.82e-115 341
MS.gene065785.t1 AT2G27420 48.159 353 160 7 9 343 1 348 2.78e-114 335
MS.gene065785.t1 AT3G49340 51.682 327 146 6 24 343 20 341 3.49e-114 335
MS.gene065785.t1 AT1G06260 52.353 340 149 7 8 341 9 341 4.89e-114 335
MS.gene065785.t1 AT4G36880 50.459 327 146 7 27 342 40 361 5.00e-111 328
MS.gene065785.t1 AT1G29090 46.176 353 173 6 4 343 7 355 9.84e-111 327
MS.gene065785.t1 AT1G20850 50.779 321 151 5 25 342 37 353 1.35e-110 326
MS.gene065785.t1 AT3G19400 50.814 307 146 5 39 342 44 348 2.23e-110 326
MS.gene065785.t1 AT1G47128 51.780 309 136 7 39 341 50 351 8.51e-110 328
MS.gene065785.t1 AT1G29080 45.773 343 164 7 14 343 13 346 1.32e-106 316
MS.gene065785.t1 AT1G09850 50.654 306 147 4 37 341 30 332 9.64e-103 309
MS.gene065785.t1 AT4G23520 48.544 309 144 8 40 341 48 348 5.87e-100 299
MS.gene065785.t1 AT4G11310 44.286 350 169 9 9 341 11 351 4.69e-98 295
MS.gene065785.t1 AT4G11320 46.349 315 143 7 40 341 57 358 1.04e-96 291
MS.gene065785.t1 AT4G11320 46.349 315 143 7 40 341 57 358 1.04e-96 291
MS.gene065785.t1 AT1G29110 40.816 343 167 7 14 342 13 333 2.09e-87 266
MS.gene065785.t1 AT3G43960 44.277 332 174 8 15 341 20 345 6.05e-85 261
MS.gene065785.t1 AT4G35350 48.375 277 127 8 9 276 17 286 1.32e-83 255
MS.gene065785.t1 AT3G19400 48.963 241 119 4 39 276 44 283 1.48e-76 237
MS.gene065785.t1 AT3G48350 47.331 281 131 8 9 276 1 277 1.80e-76 237
MS.gene065785.t1 AT5G60360 43.648 307 157 9 41 342 61 356 4.86e-76 238
MS.gene065785.t1 AT5G60360 43.322 307 157 10 41 342 61 355 1.56e-73 231
MS.gene065785.t1 AT3G45310 39.368 348 182 11 1 343 34 357 2.85e-73 231
MS.gene065785.t1 AT5G60360 42.953 298 154 9 41 333 61 347 9.36e-72 227
MS.gene065785.t1 AT3G45310 39.080 348 182 12 1 343 34 356 1.06e-70 224
MS.gene065785.t1 AT2G21430 37.188 320 171 9 30 334 45 349 1.58e-63 206
MS.gene065785.t1 AT4G39090 38.344 326 163 12 28 334 46 352 3.67e-63 205
MS.gene065785.t1 AT3G54940 38.750 320 158 10 38 334 50 354 1.13e-60 199
MS.gene065785.t1 AT4G16190 38.907 311 158 11 44 334 60 358 5.61e-60 197
MS.gene065785.t1 AT3G54940 39.056 233 124 5 38 258 50 276 3.73e-45 156
MS.gene065785.t1 AT1G02305 29.778 225 130 7 131 334 116 333 6.95e-24 101
MS.gene065785.t1 AT4G01610 27.063 303 178 12 65 338 46 334 4.01e-21 93.2
MS.gene065785.t1 AT1G02300 29.384 211 123 6 144 334 146 350 7.09e-20 90.1
MS.gene065785.t1 AT4G01610 26.733 303 179 12 65 338 46 334 8.58e-20 89.4
MS.gene065785.t1 AT2G22160 40.217 92 52 2 54 144 9 98 1.18e-14 69.7

Find 91 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAAAAGTGGTGTCTTTAC+TGG 0.247785 4.2:+30775930 MS.gene065785:CDS
ACTTCCCAGTACTCAACTTA+TGG 0.259340 4.2:-30775602 None:intergenic
TGACATGTTATGTTGATTAT+TGG 0.261556 4.2:-30775027 None:intergenic
GCATCAATAGCTACGGAAAT+TGG 0.285597 4.2:-30775885 None:intergenic
AGTTCCATCAACACCTTGAT+AGG 0.302525 4.2:-30775760 None:intergenic
TGGGAAGTTGGTCTCTTTAT+CGG 0.332823 4.2:+30775617 MS.gene065785:CDS
CTTGTTATTGCCAGCATTGT+TGG 0.332876 4.2:-30775237 None:intergenic
GGGGAGAAGAAGGATATATT+AGG 0.344233 4.2:+30776057 MS.gene065785:CDS
AATAACAAGTTATACAAATT+AGG 0.348584 4.2:+30775251 MS.gene065785:CDS
TCACTATGGCAAAGTCTATA+AGG 0.352872 4.2:+30775151 MS.gene065785:CDS
GAATTCATGGGGAACTGATT+GGG 0.355868 4.2:+30776037 MS.gene065785:CDS
ATCCATGCAGTTACCATTAC+CGG 0.356686 4.2:+30775804 MS.gene065785:CDS
AAATTCATCATTCAAAATCA+TGG 0.364764 4.2:+30775714 MS.gene065785:CDS
ATTGGTTGGTTAAGAATTCA+TGG 0.370703 4.2:+30776024 MS.gene065785:CDS
AGGTTGTGAGGGTGGTCTTA+TGG 0.376813 4.2:+30775680 MS.gene065785:CDS
ACCTATTGTAGGATGTTGTT+GGG 0.383083 4.2:+30775548 MS.gene065785:intron
TTGTAGTGCTTGCTCATTGT+TGG 0.383703 4.2:-30775844 None:intergenic
CATTGACCTTGGTTCTTCAC+TGG 0.396716 4.2:-30775434 None:intergenic
TACCCCTATCAAGGTGTTGA+TGG 0.400072 4.2:+30775756 MS.gene065785:CDS
AGAATTCATGGGGAACTGAT+TGG 0.403396 4.2:+30776036 MS.gene065785:CDS
TACCTATTGTAGGATGTTGT+TGG 0.413643 4.2:+30775547 MS.gene065785:intron
GGAATCCATAAGTTGAGTAC+TGG 0.422547 4.2:+30775597 MS.gene065785:CDS
AGTAGTTGTCTTTGTGATTG+AGG 0.437424 4.2:-30775348 None:intergenic
TTTCTCCAATCCACTGCTGA+TGG 0.445961 4.2:-30775395 None:intergenic
ACTCAACTTATGGATTCCTT+CGG 0.446108 4.2:-30775592 None:intergenic
ATTCTTAACCAACCAATACT+TGG 0.453395 4.2:-30776018 None:intergenic
AGGATATATTAGGATGCAAA+GGG 0.460167 4.2:+30776067 MS.gene065785:CDS
GGAACTGATTGGGGAGAAGA+AGG 0.464169 4.2:+30776047 MS.gene065785:CDS
CATACATGGAGTGGTCATCT+TGG 0.465658 4.2:-30775106 None:intergenic
TTGCTCATGCTTCTCATACA+TGG 0.469927 4.2:-30775120 None:intergenic
GGTGTCACTGCTGTAGGTTA+TGG 0.472995 4.2:+30775978 MS.gene065785:CDS
TAATCAACATAACATGTCAT+TGG 0.474648 4.2:+30775031 MS.gene065785:CDS
AAGTTTGGAACTTAAGCAGT+AGG 0.478514 4.2:-30776140 None:intergenic
AGCATCTAGAAACAAATTCA+AGG 0.484430 4.2:+30775313 MS.gene065785:CDS
GGTTATGGCGTCGGTAATGA+TGG 0.484602 4.2:+30775993 MS.gene065785:CDS
AGATGCTATGAACTCCTCAT+TGG 0.488280 4.2:-30775297 None:intergenic
TCTATAAGGATTCTCAAGAA+AGG 0.490614 4.2:+30775165 MS.gene065785:CDS
AAGAAAGGGAGAACCGTCTC+AGG 0.495449 4.2:+30775180 MS.gene065785:CDS
GAGCAATGGATGAGTCACTA+TGG 0.497756 4.2:+30775137 MS.gene065785:CDS
CTATAAGGATTCTCAAGAAA+GGG 0.505602 4.2:+30775166 MS.gene065785:CDS
CACATCTTCATACCCGGTAA+TGG 0.507463 4.2:-30775817 None:intergenic
GCAGTGGATTGGAGAAAGAA+AGG 0.507676 4.2:+30775401 MS.gene065785:CDS
AAGGATATATTAGGATGCAA+AGG 0.511028 4.2:+30776066 MS.gene065785:CDS
AGTGCTTGCTCATTGTTGGC+AGG 0.511275 4.2:-30775840 None:intergenic
GATGCTGCTGAAGGACTTTG+TGG 0.515605 4.2:+30776095 MS.gene065785:CDS
TAAGTTGAGTACTGGGAAGT+TGG 0.520530 4.2:+30775605 MS.gene065785:CDS
GTGTTCCATCAGCAGTGGAT+TGG 0.522985 4.2:+30775390 MS.gene065785:CDS
TTGGTTGGTTAAGAATTCAT+GGG 0.526242 4.2:+30776025 MS.gene065785:CDS
GACACTTACCACATTGACCT+TGG 0.526963 4.2:-30775445 None:intergenic
ATTGAAGCTTCCAACAATGC+TGG 0.532605 4.2:+30775227 MS.gene065785:CDS
CTTGTTGATTGTGACACAAA+AGG 0.533495 4.2:+30775648 MS.gene065785:CDS
GTTCCATCAACACCTTGATA+GGG 0.534909 4.2:-30775759 None:intergenic
TCTGCTGTTGCGGCAACCGA+AGG 0.538022 4.2:+30775576 MS.gene065785:CDS
GAATCCATAAGTTGAGTACT+GGG 0.546014 4.2:+30775598 MS.gene065785:CDS
TCCGTAGCTATTGATGCTAG+TGG 0.550787 4.2:+30775891 MS.gene065785:CDS
GAAGCTCAATACCCCTATCA+AGG 0.559311 4.2:+30775747 MS.gene065785:CDS
CCGTAGCTATTGATGCTAGT+GGG 0.567886 4.2:+30775892 MS.gene065785:CDS
GTAATGATGGAACCAAGTAT+TGG 0.568388 4.2:+30776006 MS.gene065785:CDS
ATGTTGATTATTGGCAGCCA+TGG 0.568696 4.2:-30775018 None:intergenic
TGATGGAACCAAGTATTGGT+TGG 0.568906 4.2:+30776010 MS.gene065785:CDS
AATTCATGGGGAACTGATTG+GGG 0.571940 4.2:+30776038 MS.gene065785:CDS
AGTTTGGAACTTAAGCAGTA+GGG 0.572870 4.2:-30776139 None:intergenic
AGGGGAGTCGATGCTGCTGA+AGG 0.575211 4.2:+30776086 MS.gene065785:CDS
TTTCTTTGAATATCCTGAGA+CGG 0.585323 4.2:-30775193 None:intergenic
AGGACTTTGTGGCATTGCAA+TGG 0.587668 4.2:+30776106 MS.gene065785:CDS
GGTGTAGACCAAGGTTGTGA+GGG 0.591635 4.2:+30775669 MS.gene065785:CDS
TAAGACCACCCTCACAACCT+TGG 0.592405 4.2:-30775677 None:intergenic
AGGTGTAGACCAAGGTTGTG+AGG 0.594253 4.2:+30775668 MS.gene065785:CDS
GTAGACCAAGGTTGTGAGGG+TGG 0.595756 4.2:+30775672 MS.gene065785:CDS
CCCACTAGCATCAATAGCTA+CGG 0.596676 4.2:-30775892 None:intergenic
GGTAATGGTAACTGCATGGA+TGG 0.597553 4.2:-30775802 None:intergenic
ACCCGGTAATGGTAACTGCA+TGG 0.598149 4.2:-30775806 None:intergenic
GCATCTAGAAACAAATTCAA+GGG 0.601852 4.2:+30775314 MS.gene065785:CDS
TGCAAGTGTTCCATCAGCAG+TGG 0.603452 4.2:+30775385 MS.gene065785:CDS
TTCCATCAACACCTTGATAG+GGG 0.606076 4.2:-30775758 None:intergenic
GGCAGGCACATCTTCATACC+CGG 0.606676 4.2:-30775823 None:intergenic
TCCATGCAGTTACCATTACC+GGG 0.614442 4.2:+30775805 MS.gene065785:CDS
CATGCTTCTCATACATGGAG+TGG 0.622290 4.2:-30775115 None:intergenic
TGGTTGGTTAAGAATTCATG+GGG 0.622660 4.2:+30776026 MS.gene065785:CDS
GACCATGGTGTCACTGCTGT+AGG 0.625316 4.2:+30775972 MS.gene065785:CDS
ACTGCTGTAGGTTATGGCGT+CGG 0.632447 4.2:+30775984 MS.gene065785:CDS
GGTGTCTTTACTGGTTCATG+TGG 0.637602 4.2:+30775939 MS.gene065785:CDS
CTATGAACTCCTCATTGGTG+AGG 0.645140 4.2:-30775292 None:intergenic
AACCTACAGCAGTGACACCA+TGG 0.646830 4.2:-30775974 None:intergenic
TGTATGAGAAGCATGAGCAA+TGG 0.663906 4.2:+30775123 MS.gene065785:CDS
ATTTGCAGACCTCACCAATG+AGG 0.664553 4.2:+30775283 MS.gene065785:CDS
GGATATATTAGGATGCAAAG+GGG 0.671421 4.2:+30776068 MS.gene065785:CDS
GTGAAGAACCAAGGTCAATG+TGG 0.671761 4.2:+30775437 MS.gene065785:CDS
GACACAAAAGGTGTAGACCA+AGG 0.707755 4.2:+30775660 MS.gene065785:CDS
GTAACACCAGTGAAGAACCA+AGG 0.718576 4.2:+30775428 MS.gene065785:CDS
TGTGGAACTGAGTTAGACCA+TGG 0.766973 4.2:+30775957 MS.gene065785:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACAAGTTATACAAATT+AGG + chr4.2:30775251-30775270 MS.gene065785:CDS 15.0%
!!! AATTTTAATTTACCTATTGT+AGG + chr4.2:30775537-30775556 MS.gene065785:intron 15.0%
!! AAATTCATCATTCAAAATCA+TGG + chr4.2:30775714-30775733 MS.gene065785:CDS 20.0%
! TAATCAACATAACATGTCAT+TGG + chr4.2:30775031-30775050 MS.gene065785:CDS 25.0%
! TTAGATCACATAGTATGTAA+TGG - chr4.2:30775491-30775510 None:intergenic 25.0%
!! TGACATGTTATGTTGATTAT+TGG - chr4.2:30775030-30775049 None:intergenic 25.0%
AAGGATATATTAGGATGCAA+AGG + chr4.2:30776066-30776085 MS.gene065785:CDS 30.0%
AGCATCTAGAAACAAATTCA+AGG + chr4.2:30775313-30775332 MS.gene065785:CDS 30.0%
AGGATATATTAGGATGCAAA+GGG + chr4.2:30776067-30776086 MS.gene065785:CDS 30.0%
ATTCTTAACCAACCAATACT+TGG - chr4.2:30776021-30776040 None:intergenic 30.0%
CTATAAGGATTCTCAAGAAA+GGG + chr4.2:30775166-30775185 MS.gene065785:CDS 30.0%
GCATCTAGAAACAAATTCAA+GGG + chr4.2:30775314-30775333 MS.gene065785:CDS 30.0%
TCTATAAGGATTCTCAAGAA+AGG + chr4.2:30775165-30775184 MS.gene065785:CDS 30.0%
TTTCTTTGAATATCCTGAGA+CGG - chr4.2:30775196-30775215 None:intergenic 30.0%
! ATTGGTTGGTTAAGAATTCA+TGG + chr4.2:30776024-30776043 MS.gene065785:CDS 30.0%
! GACTTTCAGTTTTACAAAAG+TGG + chr4.2:30775918-30775937 MS.gene065785:CDS 30.0%
! TTGGTTGGTTAAGAATTCAT+GGG + chr4.2:30776025-30776044 MS.gene065785:CDS 30.0%
ACCTATTGTAGGATGTTGTT+GGG + chr4.2:30775548-30775567 MS.gene065785:intron 35.0%
ACTCAACTTATGGATTCCTT+CGG - chr4.2:30775595-30775614 None:intergenic 35.0%
AGTAGTTGTCTTTGTGATTG+AGG - chr4.2:30775351-30775370 None:intergenic 35.0%
GGATATATTAGGATGCAAAG+GGG + chr4.2:30776068-30776087 MS.gene065785:CDS 35.0%
TACAAAAGTGGTGTCTTTAC+TGG + chr4.2:30775930-30775949 MS.gene065785:CDS 35.0%
TACCTATTGTAGGATGTTGT+TGG + chr4.2:30775547-30775566 MS.gene065785:intron 35.0%
TCACTATGGCAAAGTCTATA+AGG + chr4.2:30775151-30775170 MS.gene065785:CDS 35.0%
! CTTGTTGATTGTGACACAAA+AGG + chr4.2:30775648-30775667 MS.gene065785:CDS 35.0%
! GAATCCATAAGTTGAGTACT+GGG + chr4.2:30775598-30775617 MS.gene065785:CDS 35.0%
! GTAATGATGGAACCAAGTAT+TGG + chr4.2:30776006-30776025 MS.gene065785:CDS 35.0%
! TGGTTGGTTAAGAATTCATG+GGG + chr4.2:30776026-30776045 MS.gene065785:CDS 35.0%
!! ATTGGCATTGCTTTTATGCT+TGG + chr4.2:30775049-30775068 MS.gene065785:CDS 35.0%
!! TTGGCATTGCTTTTATGCTT+GGG + chr4.2:30775050-30775069 MS.gene065785:CDS 35.0%
AATTCATGGGGAACTGATTG+GGG + chr4.2:30776038-30776057 MS.gene065785:CDS 40.0%
ACTTCCCAGTACTCAACTTA+TGG - chr4.2:30775605-30775624 None:intergenic 40.0%
AGAATTCATGGGGAACTGAT+TGG + chr4.2:30776036-30776055 MS.gene065785:CDS 40.0%
AGATGCTATGAACTCCTCAT+TGG - chr4.2:30775300-30775319 None:intergenic 40.0%
AGTTCCATCAACACCTTGAT+AGG - chr4.2:30775763-30775782 None:intergenic 40.0%
ATCCATGCAGTTACCATTAC+CGG + chr4.2:30775804-30775823 MS.gene065785:CDS 40.0%
ATTGAAGCTTCCAACAATGC+TGG + chr4.2:30775227-30775246 MS.gene065785:CDS 40.0%
GAATTCATGGGGAACTGATT+GGG + chr4.2:30776037-30776056 MS.gene065785:CDS 40.0%
GCATCAATAGCTACGGAAAT+TGG - chr4.2:30775888-30775907 None:intergenic 40.0%
GGGGAGAAGAAGGATATATT+AGG + chr4.2:30776057-30776076 MS.gene065785:CDS 40.0%
GTTCCATCAACACCTTGATA+GGG - chr4.2:30775762-30775781 None:intergenic 40.0%
TTCCATCAACACCTTGATAG+GGG - chr4.2:30775761-30775780 None:intergenic 40.0%
TTGCTCATGCTTCTCATACA+TGG - chr4.2:30775123-30775142 None:intergenic 40.0%
TTGTAGTGCTTGCTCATTGT+TGG - chr4.2:30775847-30775866 None:intergenic 40.0%
! GGAATCCATAAGTTGAGTAC+TGG + chr4.2:30775597-30775616 MS.gene065785:CDS 40.0%
! TGGGAAGTTGGTCTCTTTAT+CGG + chr4.2:30775617-30775636 MS.gene065785:CDS 40.0%
! TGTATGAGAAGCATGAGCAA+TGG + chr4.2:30775123-30775142 MS.gene065785:CDS 40.0%
!! CTTGTTATTGCCAGCATTGT+TGG - chr4.2:30775240-30775259 None:intergenic 40.0%
!! TAAGTTGAGTACTGGGAAGT+TGG + chr4.2:30775605-30775624 MS.gene065785:CDS 40.0%
!! TGATGGAACCAAGTATTGGT+TGG + chr4.2:30776010-30776029 MS.gene065785:CDS 40.0%
AGGACTTTGTGGCATTGCAA+TGG + chr4.2:30776106-30776125 MS.gene065785:CDS 45.0%
ATTTGCAGACCTCACCAATG+AGG + chr4.2:30775283-30775302 MS.gene065785:CDS 45.0%
CACATCTTCATACCCGGTAA+TGG - chr4.2:30775820-30775839 None:intergenic 45.0%
CATACATGGAGTGGTCATCT+TGG - chr4.2:30775109-30775128 None:intergenic 45.0%
CATGCTTCTCATACATGGAG+TGG - chr4.2:30775118-30775137 None:intergenic 45.0%
CCCACTAGCATCAATAGCTA+CGG - chr4.2:30775895-30775914 None:intergenic 45.0%
CCGTAGCTATTGATGCTAGT+GGG + chr4.2:30775892-30775911 MS.gene065785:CDS 45.0%
CTATGAACTCCTCATTGGTG+AGG - chr4.2:30775295-30775314 None:intergenic 45.0%
GAAGCTCAATACCCCTATCA+AGG + chr4.2:30775747-30775766 MS.gene065785:CDS 45.0%
GACACTTACCACATTGACCT+TGG - chr4.2:30775448-30775467 None:intergenic 45.0%
GAGCAATGGATGAGTCACTA+TGG + chr4.2:30775137-30775156 MS.gene065785:CDS 45.0%
GCAAGCACTACAAAAAGCTG+TGG + chr4.2:30775854-30775873 MS.gene065785:CDS 45.0%
GCCCAACAACATCCTACAAT+AGG - chr4.2:30775552-30775571 None:intergenic 45.0%
GTAACACCAGTGAAGAACCA+AGG + chr4.2:30775428-30775447 MS.gene065785:CDS 45.0%
GTGAAGAACCAAGGTCAATG+TGG + chr4.2:30775437-30775456 MS.gene065785:CDS 45.0%
TCCATGCAGTTACCATTACC+GGG + chr4.2:30775805-30775824 MS.gene065785:CDS 45.0%
TCCGTAGCTATTGATGCTAG+TGG + chr4.2:30775891-30775910 MS.gene065785:CDS 45.0%
TGTGGAACTGAGTTAGACCA+TGG + chr4.2:30775957-30775976 MS.gene065785:CDS 45.0%
TTTCTCCAATCCACTGCTGA+TGG - chr4.2:30775398-30775417 None:intergenic 45.0%
! CATTGACCTTGGTTCTTCAC+TGG - chr4.2:30775437-30775456 None:intergenic 45.0%
! GACACAAAAGGTGTAGACCA+AGG + chr4.2:30775660-30775679 MS.gene065785:CDS 45.0%
! GGTAATGGTAACTGCATGGA+TGG - chr4.2:30775805-30775824 None:intergenic 45.0%
! TTGGGCATTTTCTGCTGTTG+CGG + chr4.2:30775566-30775585 MS.gene065785:CDS 45.0%
!! GCAGTGGATTGGAGAAAGAA+AGG + chr4.2:30775401-30775420 MS.gene065785:CDS 45.0%
!! GGTGTCTTTACTGGTTCATG+TGG + chr4.2:30775939-30775958 MS.gene065785:CDS 45.0%
!! TACCCCTATCAAGGTGTTGA+TGG + chr4.2:30775756-30775775 MS.gene065785:CDS 45.0%
AACCTACAGCAGTGACACCA+TGG - chr4.2:30775977-30775996 None:intergenic 50.0%
AAGAAAGGGAGAACCGTCTC+AGG + chr4.2:30775180-30775199 MS.gene065785:CDS 50.0%
ACTGCTGTAGGTTATGGCGT+CGG + chr4.2:30775984-30776003 MS.gene065785:CDS 50.0%
AGGTGTAGACCAAGGTTGTG+AGG + chr4.2:30775668-30775687 MS.gene065785:CDS 50.0%
AGTGCTTGCTCATTGTTGGC+AGG - chr4.2:30775843-30775862 None:intergenic 50.0%
GATGCTGCTGAAGGACTTTG+TGG + chr4.2:30776095-30776114 MS.gene065785:CDS 50.0%
GGAACTGATTGGGGAGAAGA+AGG + chr4.2:30776047-30776066 MS.gene065785:CDS 50.0%
GGTGTAGACCAAGGTTGTGA+GGG + chr4.2:30775669-30775688 MS.gene065785:CDS 50.0%
GTGTTCCATCAGCAGTGGAT+TGG + chr4.2:30775390-30775409 MS.gene065785:CDS 50.0%
TAAGACCACCCTCACAACCT+TGG - chr4.2:30775680-30775699 None:intergenic 50.0%
TGCAAGTGTTCCATCAGCAG+TGG + chr4.2:30775385-30775404 MS.gene065785:CDS 50.0%
! ACCCGGTAATGGTAACTGCA+TGG - chr4.2:30775809-30775828 None:intergenic 50.0%
! AGGTTGTGAGGGTGGTCTTA+TGG + chr4.2:30775680-30775699 MS.gene065785:CDS 50.0%
!! GGTGTCACTGCTGTAGGTTA+TGG + chr4.2:30775978-30775997 MS.gene065785:CDS 50.0%
!! GGTTATGGCGTCGGTAATGA+TGG + chr4.2:30775993-30776012 MS.gene065785:CDS 50.0%
GGCAGGCACATCTTCATACC+CGG - chr4.2:30775826-30775845 None:intergenic 55.0%
GTAGACCAAGGTTGTGAGGG+TGG + chr4.2:30775672-30775691 MS.gene065785:CDS 55.0%
!! GACCATGGTGTCACTGCTGT+AGG + chr4.2:30775972-30775991 MS.gene065785:CDS 55.0%
AGGGGAGTCGATGCTGCTGA+AGG + chr4.2:30776086-30776105 MS.gene065785:CDS 60.0%
TCTGCTGTTGCGGCAACCGA+AGG + chr4.2:30775576-30775595 MS.gene065785:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 30775020 30776151 30775020 ID=MS.gene065785
chr4.2 mRNA 30775020 30776151 30775020 ID=MS.gene065785.t1;Parent=MS.gene065785
chr4.2 exon 30775020 30775458 30775020 ID=MS.gene065785.t1.exon1;Parent=MS.gene065785.t1
chr4.2 CDS 30775020 30775458 30775020 ID=cds.MS.gene065785.t1;Parent=MS.gene065785.t1
chr4.2 exon 30775559 30776151 30775559 ID=MS.gene065785.t1.exon2;Parent=MS.gene065785.t1
chr4.2 CDS 30775559 30776151 30775559 ID=cds.MS.gene065785.t1;Parent=MS.gene065785.t1
Gene Sequence

>MS.gene065785

ATGGCTGCCAATAATCAACATAACATGTCATTGGCATTGCTTTTATGCTTGGGATTGTTTGCTATTCAAGTCACTTCTCGTACTCTCCAAGATGACCACTCCATGTATGAGAAGCATGAGCAATGGATGAGTCACTATGGCAAAGTCTATAAGGATTCTCAAGAAAGGGAGAACCGTCTCAGGATATTCAAAGAAAATGTGAATTACATTGAAGCTTCCAACAATGCTGGCAATAACAAGTTATACAAATTAGGCATTAATCAATTTGCAGACCTCACCAATGAGGAGTTCATAGCATCTAGAAACAAATTCAAGGGTCATATGTGCTCCTCAATCACAAAGACAACTACTTTTATGTATGAAAATGCAAGTGTTCCATCAGCAGTGGATTGGAGAAAGAAAGGAGCAGTAACACCAGTGAAGAACCAAGGTCAATGTGGTAAGTGTCTATCTAATATGTAATGCATTCCATTACATACTATGTGATCTAATATTGAATTGTCATTAAACTCTAGTTAATTTTAATTTACCTATTGTAGGATGTTGTTGGGCATTTTCTGCTGTTGCGGCAACCGAAGGAATCCATAAGTTGAGTACTGGGAAGTTGGTCTCTTTATCGGAACAAGAACTTGTTGATTGTGACACAAAAGGTGTAGACCAAGGTTGTGAGGGTGGTCTTATGGATGATGCTTTCAAATTCATCATTCAAAATCATGGACTCAACACTGAAGCTCAATACCCCTATCAAGGTGTTGATGGAACTTGCAGTGCAAACAAAGCATCCATCCATGCAGTTACCATTACCGGGTATGAAGATGTGCCTGCCAACAATGAGCAAGCACTACAAAAAGCTGTGGCAAATCAACCAATTTCCGTAGCTATTGATGCTAGTGGGTCTGACTTTCAGTTTTACAAAAGTGGTGTCTTTACTGGTTCATGTGGAACTGAGTTAGACCATGGTGTCACTGCTGTAGGTTATGGCGTCGGTAATGATGGAACCAAGTATTGGTTGGTTAAGAATTCATGGGGAACTGATTGGGGAGAAGAAGGATATATTAGGATGCAAAGGGGAGTCGATGCTGCTGAAGGACTTTGTGGCATTGCAATGGAAGCATCTTACCCTACTGCTTAA
Protein sequence

>MS.gene065785.t1

MAANNQHNMSLALLLCLGLFAIQVTSRTLQDDHSMYEKHEQWMSHYGKVYKDSQERENRLRIFKENVNYIEASNNAGNNKLYKLGINQFADLTNEEFIASRNKFKGHMCSSITKTTTFMYENASVPSAVDWRKKGAVTPVKNQGQCGCCWAFSAVAATEGIHKLSTGKLVSLSEQELVDCDTKGVDQGCEGGLMDDAFKFIIQNHGLNTEAQYPYQGVDGTCSANKASIHAVTITGYEDVPANNEQALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGVGNDGTKYWLVKNSWGTDWGEEGYIRMQRGVDAAEGLCGIAMEASYPTA