Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068695.t1 | XP_003626648.1 | 100 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 6.30E-29 | 136 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068695.t1 | Q9C9Z5 | 77.4 | 62 | 14 | 0 | 1 | 62 | 1 | 62 | 2.0e-23 | 109.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068695.t1 | Q1STQ6 | 100.0 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 4.6e-29 | 136.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene068695 | 0.808646 | 2.80E-50 | -1.69E-46 |
| MS.gene049255 | MS.gene068695 | 0.820895 | 5.39E-53 | -1.69E-46 |
| MS.gene049341 | MS.gene068695 | 0.840595 | 7.91E-58 | -1.69E-46 |
| MS.gene049382 | MS.gene068695 | 0.816907 | 4.34E-52 | -1.69E-46 |
| MS.gene049756 | MS.gene068695 | 0.83075 | 2.46E-55 | -1.69E-46 |
| MS.gene050020 | MS.gene068695 | 0.80008 | 1.70E-48 | -1.69E-46 |
| MS.gene050277 | MS.gene068695 | 0.846445 | 2.16E-59 | -1.69E-46 |
| MS.gene050303 | MS.gene068695 | 0.804035 | 2.62E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene068695 | 0.810943 | 8.96E-51 | -1.69E-46 |
| MS.gene050724 | MS.gene068695 | 0.800635 | 1.31E-48 | -1.69E-46 |
| MS.gene050807 | MS.gene068695 | 0.822361 | 2.47E-53 | -1.69E-46 |
| MS.gene050815 | MS.gene068695 | 0.858865 | 6.14E-63 | -1.69E-46 |
| MS.gene050838 | MS.gene068695 | 0.800269 | 1.56E-48 | -1.69E-46 |
| MS.gene051063 | MS.gene068695 | 0.828876 | 7.05E-55 | -1.69E-46 |
| MS.gene051243 | MS.gene068695 | 0.837515 | 4.97E-57 | -1.69E-46 |
| MS.gene051245 | MS.gene068695 | 0.827274 | 1.71E-54 | -1.69E-46 |
| MS.gene051328 | MS.gene068695 | 0.84144 | 4.74E-58 | -1.69E-46 |
| MS.gene05134 | MS.gene068695 | 0.803914 | 2.78E-49 | -1.69E-46 |
| MS.gene051451 | MS.gene068695 | 0.80066 | 1.30E-48 | -1.69E-46 |
| MS.gene051577 | MS.gene068695 | 0.821469 | 3.97E-53 | -1.69E-46 |
| MS.gene051633 | MS.gene068695 | 0.811356 | 7.29E-51 | -1.69E-46 |
| MS.gene051757 | MS.gene068695 | 0.8272 | 1.78E-54 | -1.69E-46 |
| MS.gene051789 | MS.gene068695 | 0.809029 | 2.32E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene068695 | 0.803369 | 3.60E-49 | -1.69E-46 |
| MS.gene052133 | MS.gene068695 | 0.812551 | 4.01E-51 | -1.69E-46 |
| MS.gene052286 | MS.gene068695 | 0.819182 | 1.33E-52 | -1.69E-46 |
| MS.gene052302 | MS.gene068695 | 0.825713 | 4.03E-54 | -1.69E-46 |
| MS.gene052376 | MS.gene068695 | 0.822046 | 2.92E-53 | -1.69E-46 |
| MS.gene052665 | MS.gene068695 | 0.838962 | 2.11E-57 | -1.69E-46 |
| MS.gene052896 | MS.gene068695 | 0.803535 | 3.33E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene068695 | 0.801352 | 9.37E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene068695 | 0.81501 | 1.15E-51 | -1.69E-46 |
| MS.gene053109 | MS.gene068695 | 0.803997 | 2.67E-49 | -1.69E-46 |
| MS.gene053437 | MS.gene068695 | 0.81518 | 1.05E-51 | -1.69E-46 |
| MS.gene05462 | MS.gene068695 | 0.801467 | 8.88E-49 | -1.69E-46 |
| MS.gene055162 | MS.gene068695 | 0.811647 | 6.30E-51 | -1.69E-46 |
| MS.gene055441 | MS.gene068695 | 0.804851 | 1.77E-49 | -1.69E-46 |
| MS.gene055512 | MS.gene068695 | 0.820082 | 8.28E-53 | -1.69E-46 |
| MS.gene056069 | MS.gene068695 | -0.801616 | 8.28E-49 | -1.69E-46 |
| MS.gene056432 | MS.gene068695 | 0.835492 | 1.63E-56 | -1.69E-46 |
| MS.gene056580 | MS.gene068695 | 0.816902 | 4.35E-52 | -1.69E-46 |
| MS.gene056631 | MS.gene068695 | 0.837795 | 4.21E-57 | -1.69E-46 |
| MS.gene056760 | MS.gene068695 | 0.832031 | 1.19E-55 | -1.69E-46 |
| MS.gene057057 | MS.gene068695 | 0.856169 | 3.86E-62 | -1.69E-46 |
| MS.gene057094 | MS.gene068695 | 0.815014 | 1.15E-51 | -1.69E-46 |
| MS.gene057140 | MS.gene068695 | 0.86325 | 2.85E-64 | -1.69E-46 |
| MS.gene057234 | MS.gene068695 | 0.845929 | 2.99E-59 | -1.69E-46 |
| MS.gene057260 | MS.gene068695 | 0.805327 | 1.41E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene068695 | 0.805883 | 1.08E-49 | -1.69E-46 |
| MS.gene05795 | MS.gene068695 | 0.820334 | 7.25E-53 | -1.69E-46 |
| MS.gene058055 | MS.gene068695 | 0.837599 | 4.73E-57 | -1.69E-46 |
| MS.gene058084 | MS.gene068695 | 0.803905 | 2.79E-49 | -1.69E-46 |
| MS.gene058182 | MS.gene068695 | 0.835117 | 2.02E-56 | -1.69E-46 |
| MS.gene058238 | MS.gene068695 | 0.828336 | 9.52E-55 | -1.69E-46 |
| MS.gene058399 | MS.gene068695 | 0.803788 | 2.95E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene068695 | 0.888773 | 4.39E-73 | -1.69E-46 |
| MS.gene05978 | MS.gene068695 | 0.805414 | 1.35E-49 | -1.69E-46 |
| MS.gene059898 | MS.gene068695 | 0.832683 | 8.22E-56 | -1.69E-46 |
| MS.gene059937 | MS.gene068695 | 0.825723 | 4.01E-54 | -1.69E-46 |
| MS.gene06001 | MS.gene068695 | 0.840162 | 1.03E-57 | -1.69E-46 |
| MS.gene060429 | MS.gene068695 | 0.812879 | 3.40E-51 | -1.69E-46 |
| MS.gene060587 | MS.gene068695 | 0.811444 | 6.98E-51 | -1.69E-46 |
| MS.gene060619 | MS.gene068695 | 0.811427 | 7.04E-51 | -1.69E-46 |
| MS.gene060907 | MS.gene068695 | 0.8005 | 1.40E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068695.t1 | MTR_8g005360 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 1.66e-43 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068695.t1 | AT3G08610 | 75.000 | 64 | 16 | 0 | 1 | 64 | 1 | 64 | 2.40e-33 | 108 |
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGGTACCTGTTTGTTAAT+TGG | 0.273208 | 8.3:+82266729 | None:intergenic |
| TGCTGGAATGCTTTGTGTTA+TGG | 0.308874 | 8.3:-82266966 | MS.gene068695:CDS |
| GCTGGAATGCTTTGTGTTAT+GGG | 0.333642 | 8.3:-82266965 | MS.gene068695:CDS |
| TTACCTTTGGGAATAATTGC+TGG | 0.341296 | 8.3:-82266983 | MS.gene068695:CDS |
| GTTGGAAGCTATGTTACCTT+TGG | 0.352532 | 8.3:-82266996 | MS.gene068695:CDS |
| TTGGAAGCTATGTTACCTTT+GGG | 0.406774 | 8.3:-82266995 | MS.gene068695:CDS |
| CTTCTTGTCCCTACGTTCCA+TGG | 0.446698 | 8.3:+82266774 | None:intergenic |
| AAATCATTGCCGATGTGCTT+AGG | 0.473520 | 8.3:+82266808 | None:intergenic |
| AGCACATCGGCAATGATTTG+TGG | 0.486432 | 8.3:-82266804 | MS.gene068695:CDS |
| AGAGATGGCGTTGCATTGGT+TGG | 0.487344 | 8.3:-82267014 | None:intergenic |
| AGAGAGAGATGGCGTTGCAT+TGG | 0.502767 | 8.3:-82267018 | None:intergenic |
| GCACATCGGCAATGATTTGT+GGG | 0.508503 | 8.3:-82266803 | MS.gene068695:CDS |
| CGGCAATGATTTGTGGGACG+TGG | 0.531999 | 8.3:-82266797 | MS.gene068695:CDS |
| TGATTTGTGGGACGTGGCCA+TGG | 0.553811 | 8.3:-82266791 | MS.gene068695:CDS |
| GGACGTGGCCATGGAACGTA+GGG | 0.570234 | 8.3:-82266782 | MS.gene068695:CDS |
| ATTCCAGCAATTATTCCCAA+AGG | 0.601922 | 8.3:+82266980 | None:intergenic |
| TGTGGTTAGCCTAAGCACAT+CGG | 0.628887 | 8.3:-82266817 | MS.gene068695:intron |
| CTCGTCCGTGAGCAGCTCTG+TGG | 0.632697 | 8.3:+82266924 | None:intergenic |
| TACATCCACAGAGCTGCTCA+CGG | 0.640107 | 8.3:-82266929 | MS.gene068695:CDS |
| GGGACGTGGCCATGGAACGT+AGG | 0.664354 | 8.3:-82266783 | MS.gene068695:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTGAATTGAATTGAAT+TGG | - | chr8.3:82266905-82266924 | MS.gene068695:intron | 15.0% |
| !!! | TTGAATTGGATCTGTTTTTG+TGG | - | chr8.3:82266919-82266938 | MS.gene068695:intron | 30.0% |
| ATTCCAGCAATTATTCCCAA+AGG | + | chr8.3:82266777-82266796 | None:intergenic | 35.0% | |
| TTGGAAGCTATGTTACCTTT+GGG | - | chr8.3:82266759-82266778 | MS.gene068695:CDS | 35.0% | |
| ! | TTACCTTTGGGAATAATTGC+TGG | - | chr8.3:82266771-82266790 | MS.gene068695:CDS | 35.0% |
| AAATCATTGCCGATGTGCTT+AGG | + | chr8.3:82266949-82266968 | None:intergenic | 40.0% | |
| GCTGGAATGCTTTGTGTTAT+GGG | - | chr8.3:82266789-82266808 | MS.gene068695:CDS | 40.0% | |
| GTTGGAAGCTATGTTACCTT+TGG | - | chr8.3:82266758-82266777 | MS.gene068695:CDS | 40.0% | |
| TGCTGGAATGCTTTGTGTTA+TGG | - | chr8.3:82266788-82266807 | MS.gene068695:CDS | 40.0% | |
| AGCACATCGGCAATGATTTG+TGG | - | chr8.3:82266950-82266969 | MS.gene068695:CDS | 45.0% | |
| ! | GCACATCGGCAATGATTTGT+GGG | - | chr8.3:82266951-82266970 | MS.gene068695:CDS | 45.0% |
| !! | TGTGGTTAGCCTAAGCACAT+CGG | - | chr8.3:82266937-82266956 | MS.gene068695:CDS | 45.0% |
| CTTCTTGTCCCTACGTTCCA+TGG | + | chr8.3:82266983-82267002 | None:intergenic | 50.0% | |
| TACATCCACAGAGCTGCTCA+CGG | - | chr8.3:82266825-82266844 | MS.gene068695:intron | 50.0% | |
| ! | CGGCAATGATTTGTGGGACG+TGG | - | chr8.3:82266957-82266976 | MS.gene068695:CDS | 55.0% |
| ! | TGATTTGTGGGACGTGGCCA+TGG | - | chr8.3:82266963-82266982 | MS.gene068695:CDS | 55.0% |
| GGACGTGGCCATGGAACGTA+GGG | - | chr8.3:82266972-82266991 | MS.gene068695:CDS | 60.0% | |
| CTCGTCCGTGAGCAGCTCTG+TGG | + | chr8.3:82266833-82266852 | None:intergenic | 65.0% | |
| GGGACGTGGCCATGGAACGT+AGG | - | chr8.3:82266971-82266990 | MS.gene068695:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 82266744 | 82267032 | 82266744 | ID=MS.gene068695 |
| chr8.3 | mRNA | 82266744 | 82267032 | 82266744 | ID=MS.gene068695.t1;Parent=MS.gene068695 |
| chr8.3 | exon | 82266925 | 82267032 | 82266925 | ID=MS.gene068695.t1.exon1;Parent=MS.gene068695.t1 |
| chr8.3 | CDS | 82266925 | 82267032 | 82266925 | ID=cds.MS.gene068695.t1;Parent=MS.gene068695.t1 |
| chr8.3 | exon | 82266744 | 82266830 | 82266744 | ID=MS.gene068695.t1.exon2;Parent=MS.gene068695.t1 |
| chr8.3 | CDS | 82266744 | 82266830 | 82266744 | ID=cds.MS.gene068695.t1;Parent=MS.gene068695.t1 |
| Gene Sequence |
| Protein sequence |
