AlfalfaGEDB | MS.gene26288

M. sativa cultivar XinJiangDaYe / MS.gene26288

KEGG: K20185
GO: GO:0005575 GO:0031082 GO:0031083 GO:0032991
PFAM: PF06320.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26288.t1	XP_003615993.2	93.4	106	7	0	1	106	1	106	6.10E-46	193.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26288.t1	O22929	55.2	96	43	0	11	106	26	121	4.0e-22	105.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26288.t1	G7KFM0	93.4	106	7	0	1	106	1	106	4.4e-46	193.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene26288	0.831731	1.41E-55	-1.69E-46
MS.gene049382	MS.gene26288	0.822502	2.29E-53	-1.69E-46
MS.gene049756	MS.gene26288	0.850214	1.96E-60	-1.69E-46
MS.gene050226	MS.gene26288	0.817125	3.88E-52	-1.69E-46
MS.gene050227	MS.gene26288	0.808866	2.51E-50	-1.69E-46
MS.gene050228	MS.gene26288	0.813876	2.05E-51	-1.69E-46
MS.gene050237	MS.gene26288	0.801289	9.66E-49	-1.69E-46
MS.gene050303	MS.gene26288	0.803856	2.85E-49	-1.69E-46
MS.gene050623	MS.gene26288	-0.815842	7.51E-52	-1.69E-46
MS.gene050629	MS.gene26288	-0.816352	5.78E-52	-1.69E-46
MS.gene050719	MS.gene26288	0.839682	1.37E-57	-1.69E-46
MS.gene050721	MS.gene26288	0.839471	1.55E-57	-1.69E-46
MS.gene050724	MS.gene26288	0.830693	2.54E-55	-1.69E-46
MS.gene050762	MS.gene26288	0.803986	2.68E-49	-1.69E-46
MS.gene050763	MS.gene26288	0.804727	1.88E-49	-1.69E-46
MS.gene050780	MS.gene26288	0.815049	1.13E-51	-1.69E-46
MS.gene050815	MS.gene26288	0.804336	2.27E-49	-1.69E-46
MS.gene050824	MS.gene26288	0.826641	2.43E-54	-1.69E-46
MS.gene051243	MS.gene26288	0.838343	3.04E-57	-1.69E-46
MS.gene051245	MS.gene26288	0.843981	1.00E-58	-1.69E-46
MS.gene051349	MS.gene26288	0.801417	9.09E-49	-1.69E-46
MS.gene051452	MS.gene26288	0.81219	4.80E-51	-1.69E-46
MS.gene051757	MS.gene26288	0.813758	2.18E-51	-1.69E-46
MS.gene052176	MS.gene26288	0.816211	6.22E-52	-1.69E-46
MS.gene052286	MS.gene26288	0.828654	7.97E-55	-1.69E-46
MS.gene052302	MS.gene26288	0.818207	2.21E-52	-1.69E-46
MS.gene052376	MS.gene26288	0.826405	2.76E-54	-1.69E-46
MS.gene052856	MS.gene26288	0.802995	4.31E-49	-1.69E-46
MS.gene053006	MS.gene26288	0.809955	1.47E-50	-1.69E-46
MS.gene053008	MS.gene26288	0.802787	4.75E-49	-1.69E-46
MS.gene054379	MS.gene26288	0.821763	3.40E-53	-1.69E-46
MS.gene054657	MS.gene26288	0.823675	1.22E-53	-1.69E-46
MS.gene054658	MS.gene26288	0.814455	1.53E-51	-1.69E-46
MS.gene05510	MS.gene26288	0.800871	1.18E-48	-1.69E-46
MS.gene055441	MS.gene26288	0.814118	1.81E-51	-1.69E-46
MS.gene055623	MS.gene26288	0.823206	1.57E-53	-1.69E-46
MS.gene055778	MS.gene26288	0.800776	1.23E-48	-1.69E-46
MS.gene056506	MS.gene26288	0.810287	1.24E-50	-1.69E-46
MS.gene056552	MS.gene26288	0.815181	1.05E-51	-1.69E-46
MS.gene056580	MS.gene26288	0.803956	2.72E-49	-1.69E-46
MS.gene056760	MS.gene26288	0.815078	1.11E-51	-1.69E-46
MS.gene057079	MS.gene26288	0.807659	4.54E-50	-1.69E-46
MS.gene057140	MS.gene26288	0.817926	2.56E-52	-1.69E-46
MS.gene057234	MS.gene26288	0.805444	1.33E-49	-1.69E-46
MS.gene05795	MS.gene26288	0.820618	6.24E-53	-1.69E-46
MS.gene058146	MS.gene26288	0.831658	1.47E-55	-1.69E-46
MS.gene058182	MS.gene26288	0.81378	2.15E-51	-1.69E-46
MS.gene058238	MS.gene26288	0.81577	7.80E-52	-1.69E-46
MS.gene058399	MS.gene26288	0.806324	8.70E-50	-1.69E-46
MS.gene058906	MS.gene26288	0.843116	1.71E-58	-1.69E-46
MS.gene059360	MS.gene26288	0.807299	5.41E-50	-1.69E-46
MS.gene059713	MS.gene26288	0.800895	1.16E-48	-1.69E-46
MS.gene059830	MS.gene26288	0.847165	1.37E-59	-1.69E-46
MS.gene060297	MS.gene26288	0.839696	1.36E-57	-1.69E-46
MS.gene060322	MS.gene26288	0.80814	3.59E-50	-1.69E-46
MS.gene060586	MS.gene26288	0.810928	9.03E-51	-1.69E-46
MS.gene060619	MS.gene26288	0.802048	6.75E-49	-1.69E-46
MS.gene060637	MS.gene26288	0.816562	5.19E-52	-1.69E-46
MS.gene060683	MS.gene26288	0.834273	3.30E-56	-1.69E-46
MS.gene060685	MS.gene26288	0.830875	2.29E-55	-1.69E-46
MS.gene060803	MS.gene26288	0.806876	6.65E-50	-1.69E-46
MS.gene060998	MS.gene26288	0.815172	1.06E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26288.t1	MTR_5g074880	90.826	109	10	0	1	109	1	109	3.53e-68	201

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26288.t1	AT2G30330	55.294	85	38	0	22	106	37	121	8.20e-28	99.8
MS.gene26288.t1	AT2G30330	55.294	85	38	0	22	106	37	121	1.49e-27	99.0

Find 15 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGATGATGAATGTGAATT+AGG	0.250869	5.4:-59406040	None:intergenic
CGCGTCTACAACTGGACGTT+TGG	0.267025	5.4:+59406729	MS.gene26288:CDS
TAGCCCTTCGCGTCTACAAC+TGG	0.355789	5.4:+59406721	MS.gene26288:intron
AAGAAAAGCAGAACGCGTTT+CGG	0.387718	5.4:+59406112	MS.gene26288:CDS
GCGTCTACAACTGGACGTTT+GGG	0.446986	5.4:+59406730	MS.gene26288:CDS
TTCGAGATTTGTGAAACAAA+CGG	0.473259	5.4:+59406229	MS.gene26288:CDS
TTCTCTTAACACTGCTCTCA+AGG	0.509830	5.4:+59406274	MS.gene26288:CDS
ACGCGCAGAATCAAAGGGAT+TGG	0.518596	5.4:+59406001	MS.gene26288:CDS
TAACACACGCGCAGAATCAA+AGG	0.527349	5.4:+59405995	MS.gene26288:CDS
CTGTTAGTAGAAGCAGTGAA+TGG	0.564549	5.4:+59406137	MS.gene26288:CDS
AACACACGCGCAGAATCAAA+GGG	0.580106	5.4:+59405996	MS.gene26288:CDS
TTGTGAAACAAACGGATCAG+TGG	0.597090	5.4:+59406237	MS.gene26288:CDS
ACGTCCAGTTGTAGACGCGA+AGG	0.599148	5.4:-59406725	None:intergenic
CGTCCAGTTGTAGACGCGAA+GGG	0.609212	5.4:-59406724	None:intergenic
TTAGTAGAAGCAGTGAATGG+CGG	0.707209	5.4:+59406140	MS.gene26288:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTAGTTAATAATGATA+AGG	+	chr5.4:59406506-59406525	MS.gene26288:intron	10.0%
!!	ATTGATAAGTTAGCTTATAG+CGG	+	chr5.4:59406389-59406408	MS.gene26288:intron	25.0%
	CTGATTGAATTTGTAGTGTT+TGG	+	chr5.4:59406451-59406470	MS.gene26288:intron	30.0%
	TTCGAGATTTGTGAAACAAA+CGG	+	chr5.4:59406229-59406248	MS.gene26288:CDS	30.0%
!	TGTGATGATGAATGTGAATT+AGG	-	chr5.4:59406043-59406062	None:intergenic	30.0%
	AGCTCATTTGAAAGTCTGTT+TGG	-	chr5.4:59406661-59406680	None:intergenic	35.0%
!!	TATAGCGGATGAACTTATAG+AGG	+	chr5.4:59406404-59406423	MS.gene26288:intron	35.0%
!!!	TGCATCTTTCTTCGCTTTTT+CGG	-	chr5.4:59406090-59406109	None:intergenic	35.0%
	AAGAAAAGCAGAACGCGTTT+CGG	+	chr5.4:59406112-59406131	MS.gene26288:CDS	40.0%
	ACTTCATCACGAGCTTATAG+CGG	-	chr5.4:59406563-59406582	None:intergenic	40.0%
	CTGTTAGTAGAAGCAGTGAA+TGG	+	chr5.4:59406137-59406156	MS.gene26288:CDS	40.0%
	TTAGTAGAAGCAGTGAATGG+CGG	+	chr5.4:59406140-59406159	MS.gene26288:CDS	40.0%
	TTCTCTTAACACTGCTCTCA+AGG	+	chr5.4:59406274-59406293	MS.gene26288:CDS	40.0%
	TTGTGAAACAAACGGATCAG+TGG	+	chr5.4:59406237-59406256	MS.gene26288:CDS	40.0%
!!!	CTTCGCTTTTTCGGTTTGAT+TGG	-	chr5.4:59406081-59406100	None:intergenic	40.0%
	AACACACGCGCAGAATCAAA+GGG	+	chr5.4:59405996-59406015	MS.gene26288:CDS	45.0%
	TAACACACGCGCAGAATCAA+AGG	+	chr5.4:59405995-59406014	MS.gene26288:CDS	45.0%
	ACGCGCAGAATCAAAGGGAT+TGG	+	chr5.4:59406001-59406020	MS.gene26288:CDS	50.0%
	GCGTCTACAACTGGACGTTT+GGG	+	chr5.4:59406730-59406749	MS.gene26288:CDS	50.0%
	ACGTCCAGTTGTAGACGCGA+AGG	-	chr5.4:59406728-59406747	None:intergenic	55.0%
	CGCGTCTACAACTGGACGTT+TGG	+	chr5.4:59406729-59406748	MS.gene26288:CDS	55.0%
	CGTCCAGTTGTAGACGCGAA+GGG	-	chr5.4:59406727-59406746	None:intergenic	55.0%
	TAGCCCTTCGCGTCTACAAC+TGG	+	chr5.4:59406721-59406740	MS.gene26288:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	59405978	59406759	59405978	ID=MS.gene26288
chr5.4	mRNA	59405978	59406759	59405978	ID=MS.gene26288.t1;Parent=MS.gene26288
chr5.4	exon	59405978	59406295	59405978	ID=MS.gene26288.t1.exon1;Parent=MS.gene26288.t1
chr5.4	CDS	59405978	59406295	59405978	ID=cds.MS.gene26288.t1;Parent=MS.gene26288.t1
chr5.4	exon	59406724	59406759	59406724	ID=MS.gene26288.t1.exon2;Parent=MS.gene26288.t1
chr5.4	CDS	59406724	59406759	59406724	ID=cds.MS.gene26288.t1;Parent=MS.gene26288.t1

Gene Sequence

>MS.gene26288

ATGGAAGAACGCGATGTTAACACACGCGCAGAATCAAAGGGATTGGAAGATTCATTACAAAACCTAATTCACATTCATCATCACAAATCTCTCATTCTCACCAATCAAACCGAAAAAGCGAAGAAAGATGCAATAAGAAAAGCAGAACGCGTTTCGGATCTGTTAGTAGAAGCAGTGAATGGCGGTGTTCAAGATTCGTTTATTTATCAGAAGAGAATTGAGCTTGAAATTCGTGCTCTTGCTGTAACTATTTCGAGATTTGTGAAACAAACGGATCAGTGGCTTAATGCTACTCATTCTCTTAACACTGCTCTCAAGGTAACAAATTGCTTTCATTTTTTGTGTATTCTTTAATTTTGTGTTTTCTTCATTATTGTGTTCAAATCTCTGCTTTGTGAAAAATAGCGAATAATTGATAAGTTAGCTTATAGCGGATGAACTTATAGAGGAGAGTTGATTAGCAAATAAGCTAGCTGATTGAATTTGTAGTGTTTGGTAAAATTAACTGTTCAACTTTCTAAAGATTCAATTTTTAGTTAATAATGATAAGGTACAAGCTGCTTGAATTAGCTTTATCAAAAACCGCTATAAGCTCGTGATGAAGTGACGATTACAAAACATGTCTGTTTTAGTTTAATGAGCTTATTAGACATAAGCTATAAGCTCAAAAACATGTCTTACCAAACAGACTTTCAAATGAGCTACTTGTTAAGTGACAAATGACTTTCAAGTTTCAACATACATAGCCCTTCGCGTCTACAACTGGACGTTTGGGAGCGTGA

Protein sequence

>MS.gene26288.t1

MEERDVNTRAESKGLEDSLQNLIHIHHHKSLILTNQTEKAKKDAIRKAERVSDLLVEAVNGGVQDSFIYQKRIELEIRALAVTISRFVKQTDQWLNATHSLNTALKPFASTTGRLGA