AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28929


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28929.t1 MTR_4g076090 98.529 68 1 0 1 68 1 68 4.30e-45 138
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28929.t1 AT3G22210 60.938 64 24 1 5 68 6 68 4.17e-20 75.9

Find 15 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATTCGCTCTTCCAGTTT+TGG 0.231911 4.4:-34385179 MS.gene28929:CDS
GTCTTCTAGCTCGCCTTTCT+CGG 0.289922 4.4:+34384949 None:intergenic
AGCTCGCCTTTCTCGGGAGC+TGG 0.345325 4.4:+34384956 None:intergenic
GACAGAAATCACTTCAAGAT+TGG 0.349085 4.4:-34385019 MS.gene28929:intron
GCTGCAACAATGCCCAAAAC+TGG 0.365881 4.4:+34385166 None:intergenic
TCTTCTAGCTCGCCTTTCTC+GGG 0.457167 4.4:+34384950 None:intergenic
CTTTCTCGGGAGCTGGATGT+TGG 0.468532 4.4:+34384963 None:intergenic
ATGATTCTGAATCAGACAAT+GGG 0.472193 4.4:-34384995 MS.gene28929:CDS
ACAGAAATCACTTCAAGATT+GGG 0.482353 4.4:-34385018 MS.gene28929:intron
ATTGAAACTCACTGCATAGA+AGG 0.485260 4.4:+34385132 None:intergenic
CAACATCCAGCTCCCGAGAA+AGG 0.508532 4.4:-34384962 MS.gene28929:CDS
GATGATTCTGAATCAGACAA+TGG 0.593424 4.4:-34384996 MS.gene28929:CDS
GAGTTTCAATGAGATTAGAG+AGG 0.641117 4.4:-34385119 MS.gene28929:intron
TGATTCTGAATCAGACAATG+GGG 0.677451 4.4:-34384994 MS.gene28929:CDS
GATTCTGAATCAGACAATGG+GGG 0.690393 4.4:-34384993 MS.gene28929:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAGAAATCACTTCAAGATT+GGG - chr4.4:34385095-34385114 MS.gene28929:intron 30.0%
ATGATTCTGAATCAGACAAT+GGG - chr4.4:34385118-34385137 MS.gene28929:intron 30.0%
ATTGAAACTCACTGCATAGA+AGG + chr4.4:34384984-34385003 None:intergenic 35.0%
GACAGAAATCACTTCAAGAT+TGG - chr4.4:34385094-34385113 MS.gene28929:intron 35.0%
GAGTTTCAATGAGATTAGAG+AGG - chr4.4:34384994-34385013 MS.gene28929:CDS 35.0%
GATGATTCTGAATCAGACAA+TGG - chr4.4:34385117-34385136 MS.gene28929:intron 35.0%
TGATTCTGAATCAGACAATG+GGG - chr4.4:34385119-34385138 MS.gene28929:intron 35.0%
GATTCTGAATCAGACAATGG+GGG - chr4.4:34385120-34385139 MS.gene28929:CDS 40.0%
! CAATTCGCTCTTCCAGTTTT+GGG - chr4.4:34384935-34384954 MS.gene28929:CDS 40.0%
! GCAATTCGCTCTTCCAGTTT+TGG - chr4.4:34384934-34384953 MS.gene28929:CDS 45.0%
GCTGCAACAATGCCCAAAAC+TGG + chr4.4:34384950-34384969 None:intergenic 50.0%
!! GTCTTCTAGCTCGCCTTTCT+CGG + chr4.4:34385167-34385186 None:intergenic 50.0%
!! TCTTCTAGCTCGCCTTTCTC+GGG + chr4.4:34385166-34385185 None:intergenic 50.0%
CAACATCCAGCTCCCGAGAA+AGG - chr4.4:34385151-34385170 MS.gene28929:CDS 55.0%
CTTTCTCGGGAGCTGGATGT+TGG + chr4.4:34385153-34385172 None:intergenic 55.0%
AGCTCGCCTTTCTCGGGAGC+TGG + chr4.4:34385160-34385179 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 34384923 34385212 34384923 ID=MS.gene28929
chr4.4 mRNA 34384923 34385212 34384923 ID=MS.gene28929.t1;Parent=MS.gene28929
chr4.4 exon 34385120 34385212 34385120 ID=MS.gene28929.t1.exon1;Parent=MS.gene28929.t1
chr4.4 CDS 34385120 34385212 34385120 ID=cds.MS.gene28929.t1;Parent=MS.gene28929.t1
chr4.4 exon 34384923 34385036 34384923 ID=MS.gene28929.t1.exon2;Parent=MS.gene28929.t1
chr4.4 CDS 34384923 34385036 34384923 ID=cds.MS.gene28929.t1;Parent=MS.gene28929.t1
Gene Sequence

>MS.gene28929

ATGGATGCAATGCAATTCGCTCTTCCAGTTTTGGGCATTGTTGCAGCTGCAGCTGTGACCTTCTATGCAGTGAGTTTCAATGAGATTAGAGAGGTAAGTCACTAAGCAGCATCATCAACTATGTAATATATATTACATGATATCTTTATCAATCTTATAAAATTGAATTATGACAGAAATCACTTCAAGATTGGGATGATTCTGAATCAGACAATGGGGGTTTTAGACCAACATCCAGCTCCCGAGAAAGGCGAGCTAGAAGACAAGCTAGTAAAAATACCAAAAAATAA

Protein sequence

>MS.gene28929.t1

MDAMQFALPVLGIVAAAAVTFYAVSFNEIREKSLQDWDDSESDNGGFRPTSSSRERRARRQASKNTKK