AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54215

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54215.t1 MTR_5g013900 100.000 207 0 0 1 207 1 207 1.49e-153 424
MS.gene54215.t1 MTR_4g098950 91.304 207 17 1 1 207 1 206 1.43e-140 391
MS.gene54215.t1 MTR_8g090215 84.058 207 33 0 1 207 1 207 1.28e-130 365
MS.gene54215.t1 MTR_3g087160 84.058 207 32 1 1 207 1 206 5.28e-129 362
MS.gene54215.t1 MTR_1g094220 47.561 164 85 1 9 171 31 194 1.12e-50 163
MS.gene54215.t1 MTR_1g094220 47.170 159 83 1 9 166 31 189 2.93e-48 157
MS.gene54215.t1 MTR_1g094220 47.436 156 81 1 9 163 31 186 2.37e-47 154
MS.gene54215.t1 MTR_1g094220 47.436 156 81 1 9 163 31 186 3.63e-47 155
MS.gene54215.t1 MTR_8g031680 44.099 161 90 0 10 170 7 167 5.03e-47 154
MS.gene54215.t1 MTR_3g064390 40.341 176 105 0 11 186 12 187 1.06e-46 152
MS.gene54215.t1 MTR_5g076590 39.773 176 106 0 11 186 12 187 1.81e-46 152
MS.gene54215.t1 MTR_8g103550 43.750 160 90 0 11 170 11 170 1.46e-45 150
MS.gene54215.t1 MTR_2g102060 41.618 173 97 1 3 171 4 176 2.69e-45 149
MS.gene54215.t1 MTR_8g031870 44.304 158 88 0 10 167 7 164 5.46e-45 148
MS.gene54215.t1 MTR_4g064897 36.893 206 117 2 10 206 13 214 7.79e-45 148
MS.gene54215.t1 MTR_2g005500 43.478 161 91 0 10 170 10 170 1.78e-44 147
MS.gene54215.t1 MTR_3g069510 35.922 206 120 2 10 206 13 215 8.21e-44 146
MS.gene54215.t1 MTR_0262s0050 41.401 157 92 0 10 166 16 172 1.71e-43 145
MS.gene54215.t1 MTR_8g031660 42.857 161 92 0 10 170 7 167 1.89e-43 145
MS.gene54215.t1 MTR_8g089985 40.909 154 91 0 10 163 18 171 5.07e-43 144
MS.gene54215.t1 MTR_2g005510 41.290 155 91 0 10 164 16 170 6.58e-43 144
MS.gene54215.t1 MTR_3g086980 39.610 154 93 0 10 163 17 170 1.86e-42 142
MS.gene54215.t1 MTR_6g021790 35.354 198 119 3 10 204 7 198 1.91e-42 142
MS.gene54215.t1 MTR_4g012940 39.216 204 111 3 11 207 10 207 2.55e-42 142
MS.gene54215.t1 MTR_1g052475 40.127 157 94 0 10 166 16 172 2.79e-42 142
MS.gene54215.t1 MTR_7g051940 42.208 154 89 0 10 163 14 167 2.86e-42 142
MS.gene54215.t1 MTR_2g027660 36.264 182 110 1 10 191 7 182 3.86e-42 141
MS.gene54215.t1 MTR_8g027220 34.951 206 126 1 10 207 27 232 4.78e-42 142
MS.gene54215.t1 MTR_8g006970 37.563 197 117 2 10 205 23 214 5.48e-42 141
MS.gene54215.t1 MTR_4g079350 34.715 193 108 1 10 184 14 206 8.63e-42 140
MS.gene54215.t1 MTR_4g088090 33.679 193 110 1 10 184 14 206 8.82e-42 140
MS.gene54215.t1 MTR_8g036220 33.000 200 130 1 10 205 17 216 1.57e-41 140
MS.gene54215.t1 MTR_7g081700 40.373 161 96 0 10 170 9 169 3.06e-41 139
MS.gene54215.t1 MTR_4g069850 38.756 209 104 6 11 207 10 206 5.51e-41 138
MS.gene54215.t1 MTR_8g031830 41.830 153 89 0 10 162 7 159 8.50e-41 137
MS.gene54215.t1 MTR_2g014990 38.854 157 96 0 10 166 14 170 9.00e-41 138
MS.gene54215.t1 MTR_8g006970 40.373 161 96 0 10 170 9 169 1.09e-40 137
MS.gene54215.t1 MTR_1g078200 39.490 157 95 0 10 166 15 171 1.24e-40 138
MS.gene54215.t1 MTR_2g075950 37.580 157 98 0 10 166 14 170 1.49e-40 137
MS.gene54215.t1 MTR_7g405790 39.490 157 95 0 10 166 16 172 3.68e-40 137
MS.gene54215.t1 MTR_2g101890 37.745 204 114 3 11 207 12 209 3.76e-40 136
MS.gene54215.t1 MTR_6g079550 40.909 154 91 0 10 163 13 166 1.65e-39 135
MS.gene54215.t1 MTR_2g083860 36.943 157 99 0 10 166 14 170 1.86e-39 135
MS.gene54215.t1 MTR_1g115325 36.869 198 121 3 11 205 17 213 2.22e-39 134
MS.gene54215.t1 MTR_1g068740 37.143 210 125 4 3 205 5 214 3.02e-39 134
MS.gene54215.t1 MTR_1g068730 37.143 210 125 4 3 205 5 214 5.46e-39 133
MS.gene54215.t1 MTR_2g090865 34.694 196 109 2 10 187 12 206 3.70e-38 131
MS.gene54215.t1 MTR_7g100020 41.139 158 92 1 11 167 17 174 3.65e-37 129
MS.gene54215.t1 MTR_2g096550 36.634 202 115 5 11 205 17 212 3.75e-37 129
MS.gene54215.t1 MTR_8g092020 36.538 208 109 6 11 207 14 209 5.53e-37 128
MS.gene54215.t1 MTR_4g063160 36.683 199 122 3 11 205 17 215 5.86e-37 128
MS.gene54215.t1 MTR_3g064390 43.200 125 71 0 11 135 12 136 1.47e-36 125
MS.gene54215.t1 MTR_8g093740 36.869 198 119 4 10 205 14 207 8.12e-36 125
MS.gene54215.t1 MTR_1g097420 33.333 204 123 4 11 207 10 207 7.75e-35 122
MS.gene54215.t1 MTR_4g099470 36.207 174 106 1 32 205 7 175 9.29e-35 121
MS.gene54215.t1 MTR_7g117910 40.476 126 75 0 10 135 21 146 9.95e-35 122
MS.gene54215.t1 MTR_3g096350 35.922 206 110 5 10 205 13 206 5.14e-34 120
MS.gene54215.t1 MTR_1g062760 39.375 160 94 3 10 167 15 173 5.74e-33 118
MS.gene54215.t1 MTR_7g103070 36.875 160 99 2 10 167 17 176 1.87e-32 117
MS.gene54215.t1 MTR_1g090717 40.000 160 93 3 10 166 16 175 1.92e-32 116
MS.gene54215.t1 MTR_8g107070 37.662 154 96 0 10 163 15 168 2.79e-32 117
MS.gene54215.t1 MTR_1g029450 34.673 199 126 4 10 206 18 214 8.33e-32 115
MS.gene54215.t1 MTR_1g097420 31.707 205 125 5 10 207 38 234 3.26e-31 114
MS.gene54215.t1 MTR_1g068730 37.956 137 81 1 2 134 4 140 3.13e-29 107
MS.gene54215.t1 MTR_1g068740 38.519 135 79 1 2 132 4 138 4.70e-29 107
MS.gene54215.t1 MTR_8g093740 34.911 169 105 3 40 206 10 175 3.79e-26 99.4
MS.gene54215.t1 MTR_3g107707 31.977 172 106 3 10 177 14 178 4.20e-26 100
MS.gene54215.t1 MTR_3g107717 31.977 172 106 3 10 177 14 178 4.38e-26 100
MS.gene54215.t1 MTR_3g107713 33.333 168 109 3 10 177 14 178 4.72e-26 100
MS.gene54215.t1 MTR_6g039490 29.412 204 129 4 11 207 10 205 1.64e-25 99.4
MS.gene54215.t1 MTR_3g096350 40.945 127 70 4 10 132 13 138 2.03e-25 96.7
MS.gene54215.t1 MTR_3g096350 42.063 126 70 3 10 133 13 137 8.54e-25 95.5
MS.gene54215.t1 MTR_7g103070 40.351 114 66 2 56 167 7 120 7.55e-23 90.5
MS.gene54215.t1 MTR_2g090875 35.758 165 92 5 11 163 8 170 4.17e-22 89.4
MS.gene54215.t1 MTR_3g078260 35.758 165 92 5 11 163 10 172 7.21e-22 89.4
MS.gene54215.t1 MTR_4g088055 35.152 165 93 5 11 163 8 170 2.01e-21 87.8
MS.gene54215.t1 MTR_2g005500 31.056 161 71 1 10 170 10 130 7.34e-21 85.5
MS.gene54215.t1 MTR_8g075240 32.727 165 97 5 11 163 8 170 3.08e-20 84.7
MS.gene54215.t1 MTR_6g087980 33.939 165 95 5 11 163 8 170 3.67e-20 84.7
MS.gene54215.t1 MTR_5g022600 32.727 165 97 5 11 163 8 170 3.68e-20 84.3
MS.gene54215.t1 MTR_4g113620 30.952 168 104 4 10 177 14 169 6.72e-20 84.0
MS.gene54215.t1 MTR_4g073250 32.121 165 98 5 11 163 8 170 4.12e-19 81.6
MS.gene54215.t1 MTR_1452s0010 46.154 78 42 0 29 106 1 78 5.20e-19 79.0
MS.gene54215.t1 MTR_5g092060 27.485 171 118 3 5 170 100 269 2.45e-16 75.9
MS.gene54215.t1 MTR_3g078260 38.596 114 67 3 11 123 10 121 2.99e-16 72.8
MS.gene54215.t1 MTR_1686s0010 39.744 78 47 0 29 106 1 78 6.28e-16 70.5
MS.gene54215.t1 MTR_4g047870 24.260 169 122 2 7 170 89 256 2.91e-12 64.3
MS.gene54215.t1 MTR_8g031810 26.733 101 60 1 10 110 7 93 5.22e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54215.t1 AT2G44610 92.788 208 14 1 1 207 1 208 6.55e-143 397
MS.gene54215.t1 AT5G10260 86.957 207 27 0 1 207 1 207 1.48e-133 373
MS.gene54215.t1 AT2G22290 83.092 207 35 0 1 207 1 207 2.09e-127 357
MS.gene54215.t1 AT4G39890 76.636 214 43 3 1 207 1 214 2.87e-114 325
MS.gene54215.t1 AT5G64990 76.733 202 40 2 10 207 8 206 1.58e-106 305
MS.gene54215.t1 AT5G64990 74.163 209 40 3 10 207 8 213 9.28e-104 298
MS.gene54215.t1 AT3G54840 47.561 164 85 1 9 171 33 196 2.48e-50 162
MS.gene54215.t1 AT3G54840 47.826 161 83 1 12 171 2 162 1.57e-49 159
MS.gene54215.t1 AT4G19640 42.857 161 92 0 11 171 12 172 3.90e-48 156
MS.gene54215.t1 AT3G54840 48.077 156 80 1 9 163 33 188 4.15e-48 156
MS.gene54215.t1 AT5G45130 42.857 161 92 0 11 171 12 172 4.68e-48 156
MS.gene54215.t1 AT5G45130 42.138 159 92 0 13 171 22 180 4.90e-46 151
MS.gene54215.t1 AT4G17170 34.928 209 117 2 10 205 7 209 4.84e-44 146
MS.gene54215.t1 AT1G09630 41.401 157 92 0 10 166 13 169 5.21e-44 146
MS.gene54215.t1 AT1G07410 41.401 157 92 0 10 166 13 169 8.76e-44 145
MS.gene54215.t1 AT3G11730 37.811 201 113 2 10 205 9 202 9.48e-44 145
MS.gene54215.t1 AT4G17160 39.506 162 98 0 9 170 6 167 1.58e-43 145
MS.gene54215.t1 AT5G65270 38.854 157 96 0 10 166 18 174 1.72e-43 145
MS.gene54215.t1 AT5G59150 40.373 161 96 0 10 170 13 173 2.27e-43 145
MS.gene54215.t1 AT3G12160 41.401 157 92 0 10 166 16 172 5.68e-43 144
MS.gene54215.t1 AT3G46830 40.127 157 94 0 10 166 13 169 1.44e-42 143
MS.gene54215.t1 AT3G46830 40.127 157 94 0 10 166 13 169 1.44e-42 143
MS.gene54215.t1 AT3G15060 37.853 177 107 1 10 186 14 187 2.08e-42 142
MS.gene54215.t1 AT4G39990 38.217 157 97 0 10 166 18 174 2.32e-42 142
MS.gene54215.t1 AT1G05810 37.624 202 122 2 10 207 13 214 2.65e-42 142
MS.gene54215.t1 AT5G60860 34.715 193 108 1 10 184 14 206 2.94e-42 142
MS.gene54215.t1 AT1G05810 37.931 203 120 3 10 207 59 260 6.39e-42 142
MS.gene54215.t1 AT1G02130 40.373 161 96 0 10 170 9 169 1.33e-41 140
MS.gene54215.t1 AT4G35860 34.848 198 120 3 10 204 7 198 1.58e-41 140
MS.gene54215.t1 AT2G43130 36.000 200 123 2 10 205 13 211 2.20e-41 139
MS.gene54215.t1 AT2G31680 40.127 157 94 0 10 166 13 169 2.42e-41 140
MS.gene54215.t1 AT5G47520 39.490 157 95 0 10 166 15 171 2.53e-41 140
MS.gene54215.t1 AT5G47200 36.735 196 119 1 10 205 9 199 2.67e-41 139
MS.gene54215.t1 AT4G17530 37.113 194 122 0 10 203 9 202 2.72e-41 139
MS.gene54215.t1 AT3G18820 38.278 209 105 5 11 207 10 206 6.79e-41 138
MS.gene54215.t1 AT3G07410 42.208 154 89 0 10 163 13 166 8.55e-41 138
MS.gene54215.t1 AT5G47960 39.610 154 93 0 10 163 16 169 9.45e-41 138
MS.gene54215.t1 AT1G28550 38.854 157 96 0 10 166 14 170 1.27e-40 138
MS.gene54215.t1 AT1G52280 38.235 204 112 3 11 207 10 206 4.04e-40 136
MS.gene54215.t1 AT1G16920 36.628 172 106 1 10 181 14 182 5.27e-40 136
MS.gene54215.t1 AT1G18200 40.909 154 91 0 10 163 14 167 5.85e-40 136
MS.gene54215.t1 AT4G18430 33.816 207 120 3 10 205 14 214 1.66e-39 135
MS.gene54215.t1 AT1G73640 38.961 154 94 0 10 163 14 167 1.12e-38 133
MS.gene54215.t1 AT2G33870 37.580 157 98 0 10 166 14 170 1.34e-38 132
MS.gene54215.t1 AT5G45750 35.669 157 101 0 10 166 14 170 2.94e-38 132
MS.gene54215.t1 AT1G06400 36.943 157 99 0 10 166 14 170 4.48e-38 131
MS.gene54215.t1 AT3G16100 37.745 204 113 3 11 207 10 206 4.87e-38 130
MS.gene54215.t1 AT3G53610 38.384 198 118 4 11 205 17 213 5.81e-38 131
MS.gene54215.t1 AT3G53610 38.384 198 118 4 11 205 17 213 5.81e-38 131
MS.gene54215.t1 AT3G53610 38.384 198 118 4 11 205 17 213 5.81e-38 131
MS.gene54215.t1 AT1G01200 40.764 157 91 2 10 164 29 185 8.98e-38 131
MS.gene54215.t1 AT5G59840 36.041 197 124 2 11 205 17 213 1.07e-37 130
MS.gene54215.t1 AT3G46060 35.533 197 125 2 11 205 17 213 1.78e-37 129
MS.gene54215.t1 AT3G46060 35.533 197 125 2 11 205 17 213 1.78e-37 129
MS.gene54215.t1 AT3G46060 35.533 197 125 2 11 205 17 213 1.78e-37 129
MS.gene54215.t1 AT4G19640 42.400 125 72 0 11 135 12 136 4.77e-37 126
MS.gene54215.t1 AT4G18800 35.032 157 102 0 10 166 14 170 5.64e-37 128
MS.gene54215.t1 AT5G03520 35.377 212 131 3 1 206 3 214 9.15e-37 128
MS.gene54215.t1 AT5G03530 37.000 200 120 5 10 206 14 210 3.88e-36 126
MS.gene54215.t1 AT5G03530 37.000 200 120 5 10 206 14 210 3.88e-36 126
MS.gene54215.t1 AT4G19640 42.400 125 72 0 11 135 12 136 5.55e-36 125
MS.gene54215.t1 AT1G49300 36.364 209 109 6 11 207 10 206 6.57e-36 125
MS.gene54215.t1 AT1G49300 36.364 209 109 6 11 207 10 206 6.57e-36 125
MS.gene54215.t1 AT3G09900 39.037 187 109 2 1 182 3 189 1.28e-35 125
MS.gene54215.t1 AT1G22740 37.321 209 104 8 11 207 10 203 2.38e-34 121
MS.gene54215.t1 AT5G39620 34.112 214 112 6 9 207 5 204 9.79e-34 120
MS.gene54215.t1 AT1G43890 39.623 159 93 3 10 166 14 171 3.46e-33 118
MS.gene54215.t1 AT1G43890 39.623 159 93 3 10 166 14 171 3.46e-33 118
MS.gene54215.t1 AT1G43890 39.623 159 93 3 10 166 14 171 3.46e-33 118
MS.gene54215.t1 AT2G21880 37.725 167 91 3 11 167 11 174 5.42e-33 118
MS.gene54215.t1 AT4G09720 33.333 204 122 5 11 207 10 206 6.01e-33 117
MS.gene54215.t1 AT3G09910 40.625 160 92 3 10 167 14 172 7.34e-33 117
MS.gene54215.t1 AT3G09910 40.625 160 92 3 10 167 14 172 7.34e-33 117
MS.gene54215.t1 AT3G09910 40.625 160 92 3 10 167 14 172 7.34e-33 117
MS.gene54215.t1 AT3G09910 40.625 160 92 3 10 167 14 172 1.47e-32 116
MS.gene54215.t1 AT3G09910 40.625 160 92 3 10 167 14 172 1.47e-32 116
MS.gene54215.t1 AT4G09720 32.536 209 122 5 11 207 10 211 1.52e-31 114
MS.gene54215.t1 AT4G35860 36.774 155 89 3 53 204 4 152 5.33e-31 111
MS.gene54215.t1 AT4G09720 31.628 215 122 5 11 207 10 217 8.40e-31 112
MS.gene54215.t1 AT5G59150 40.678 118 70 0 53 170 5 122 5.06e-29 106
MS.gene54215.t1 AT5G03520 34.545 165 106 2 44 206 40 204 4.88e-28 105
MS.gene54215.t1 AT5G20010 31.383 188 112 4 10 187 14 194 1.61e-26 101
MS.gene54215.t1 AT5G20020 31.977 172 106 3 10 177 14 178 2.53e-26 101
MS.gene54215.t1 AT2G21880 35.526 152 85 3 26 167 1 149 5.09e-26 99.4
MS.gene54215.t1 AT2G21880 35.526 152 85 3 26 167 1 149 5.09e-26 99.4
MS.gene54215.t1 AT5G55190 31.977 172 106 3 10 177 14 178 5.62e-26 100
MS.gene54215.t1 AT5G55080 30.994 171 115 3 7 177 11 178 8.06e-26 100
MS.gene54215.t1 AT4G09720 32.203 177 106 5 38 207 3 172 6.45e-24 93.6
MS.gene54215.t1 AT4G09720 32.203 177 106 5 38 207 3 172 6.45e-24 93.6
MS.gene54215.t1 AT4G09720 32.203 177 106 5 38 207 3 172 6.45e-24 93.6
MS.gene54215.t1 AT2G21880 33.533 167 90 4 11 167 11 166 1.63e-23 93.6
MS.gene54215.t1 AT2G44690 33.939 165 95 5 11 163 20 182 9.55e-23 91.7
MS.gene54215.t1 AT3G48040 36.364 165 91 5 11 163 10 172 9.32e-22 89.0
MS.gene54215.t1 AT4G35020 33.333 165 96 5 11 163 8 170 3.93e-21 87.0
MS.gene54215.t1 AT4G35020 33.333 165 96 5 11 163 8 170 3.93e-21 87.0
MS.gene54215.t1 AT4G35020 33.333 165 96 5 11 163 8 170 3.93e-21 87.0
MS.gene54215.t1 AT1G75840 33.939 165 95 5 11 163 8 170 1.09e-20 85.9
MS.gene54215.t1 AT1G20090 33.939 165 95 5 11 163 7 169 1.17e-20 85.9
MS.gene54215.t1 AT5G62880 36.364 165 91 6 11 163 10 172 1.80e-20 85.9
MS.gene54215.t1 AT3G51300 33.333 165 96 5 11 163 8 170 3.33e-20 84.7
MS.gene54215.t1 AT4G35950 33.333 165 96 5 11 163 8 170 6.39e-20 84.0
MS.gene54215.t1 AT2G17800 33.333 165 96 5 11 163 8 170 6.95e-20 83.6
MS.gene54215.t1 AT2G17800 33.333 165 96 5 11 163 8 170 6.95e-20 83.6
MS.gene54215.t1 AT3G48040 35.897 156 86 5 11 154 10 163 7.04e-19 80.5
MS.gene54215.t1 AT4G28950 32.515 163 98 5 11 163 8 168 9.34e-19 80.9
MS.gene54215.t1 AT5G45970 32.530 166 96 6 11 163 8 170 3.41e-18 79.3
MS.gene54215.t1 AT5G62880 38.525 122 72 3 11 131 10 129 6.38e-18 78.6
MS.gene54215.t1 AT5G46025 40.964 83 48 1 85 166 20 102 2.55e-15 69.7
MS.gene54215.t1 AT4G35020 34.211 114 72 3 11 123 8 119 9.58e-15 68.9
MS.gene54215.t1 AT4G35020 34.211 114 72 3 11 123 8 119 9.58e-15 68.9
MS.gene54215.t1 AT3G21700 26.163 172 120 3 5 170 102 272 3.83e-14 69.7
MS.gene54215.t1 AT5G54840 27.108 166 113 5 11 170 103 266 8.86e-14 68.9
MS.gene54215.t1 AT3G51290 32.624 141 81 5 35 163 633 771 1.26e-12 66.6
MS.gene54215.t1 AT3G21700 25.581 172 120 4 5 170 102 271 9.78e-12 63.2

Find 39 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCCACTTTGTTGTATTT+AGG 0.181950 5.1:-6818071 None:intergenic
CAAAGCTGGATTTAATATTA+AGG 0.271646 5.1:+6819104 MS.gene54215:CDS
GAAGACAGTGTTTCCATTCC+AGG 0.314323 5.1:-6819528 None:intergenic
CTTAATATTAAATCCAGCTT+TGG 0.362326 5.1:-6819103 None:intergenic
GTAAACTTAAAGTCTTCATC+TGG 0.363108 5.1:+6819576 MS.gene54215:CDS
GAAGTACAAACTGGTGTTCT+TGG 0.394319 5.1:+6815697 MS.gene54215:CDS
TAAATTCGACAACACCTATC+AGG 0.414459 5.1:+6815769 MS.gene54215:CDS
AAAATTGCTGCTGCATTACC+TGG 0.441428 5.1:+6819510 MS.gene54215:CDS
ATGTCATTATTGTTCTTGTT+GGG 0.447960 5.1:+6818121 MS.gene54215:CDS
TGCTGCTGCATTACCTGGAA+TGG 0.467151 5.1:+6819515 MS.gene54215:CDS
TTCTGCAAAACAAGAAGACT+TGG 0.479263 5.1:+6819548 MS.gene54215:CDS
AAGTACAAACTGGTGTTCTT+GGG 0.481954 5.1:+6815698 MS.gene54215:CDS
AATGGCGCCAGTGTCAGCTT+TGG 0.485096 5.1:+6815673 None:intergenic
ATTGAAACTAGCGCCAAAGC+TGG 0.491491 5.1:+6819090 MS.gene54215:CDS
GATGTCATTATTGTTCTTGT+TGG 0.493852 5.1:+6818120 MS.gene54215:CDS
TGATAGGGATACAGCTGGAC+AGG 0.496271 5.1:+6817390 MS.gene54215:intron
AGCTTTGGCGAAGTACAAAC+TGG 0.499226 5.1:+6815688 MS.gene54215:CDS
GAATATTGAAGATACCTGAT+AGG 0.509953 5.1:-6815783 None:intergenic
TGCAACATCATATACAATAA+CGG 0.512280 5.1:-6817461 None:intergenic
GACAAGTCTCCATAGAGGAC+GGG 0.512967 5.1:+6819034 MS.gene54215:intron
CAATCTCAAACTCAATCAAG+TGG 0.517260 5.1:+6819606 MS.gene54215:CDS
GATGAGTCTCTGATGTAGCT+TGG 0.526858 5.1:-6817433 None:intergenic
TGTTCTGATAGGGATACAGC+TGG 0.529034 5.1:+6817385 MS.gene54215:intron
AAACTGATCTCGTTGAAAAG+AGG 0.533179 5.1:+6818148 MS.gene54215:CDS
TTCTTGGGTGACCAATCCGT+TGG 0.534832 5.1:+6815713 MS.gene54215:CDS
ATCATATACAATAACGGCTA+CGG 0.543543 5.1:-6817455 None:intergenic
TGAAGAGGTGCGAACAGAGA+GGG 0.556153 5.1:+6818092 MS.gene54215:CDS
TACTTCGCCAAAGCTGACAC+TGG 0.559582 5.1:-6815680 None:intergenic
TAAACTTAAAGTCTTCATCT+GGG 0.572801 5.1:+6819577 MS.gene54215:CDS
TTGAAGAGGTGCGAACAGAG+AGG 0.586465 5.1:+6818091 MS.gene54215:CDS
CTTTCCTAAATACAACAAAG+TGG 0.589578 5.1:+6818067 MS.gene54215:CDS
GAAGAGGTGCGAACAGAGAG+GGG 0.592899 5.1:+6818093 MS.gene54215:CDS
TTGTATTTAGGAAAGTCTGC+CGG 0.610534 5.1:-6818059 None:intergenic
AGACAAGTCTCCATAGAGGA+CGG 0.619191 5.1:+6819033 MS.gene54215:intron
ACAAGTCTCCATAGAGGACG+GGG 0.622928 5.1:+6819035 MS.gene54215:CDS
TACAACAAAGTGGATTGAAG+AGG 0.628316 5.1:+6818077 MS.gene54215:CDS
GAACTGTTCGACTACAGTTG+TGG 0.643989 5.1:+6817009 MS.gene54215:CDS
CATGAAGCGAGTGATGATGC+TGG 0.674864 5.1:-6815741 None:intergenic
CTGCAGACAAGTCTCCATAG+AGG 0.688178 5.1:+6819029 MS.gene54215:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATCATAATAAGTTA+AGG - chr5.1:6816928-6816947 None:intergenic 10.0%
!! TTCAAAATAAATAAATTGTT+GGG + chr5.1:6818495-6818514 MS.gene54215:intron 10.0%
!!! TTATTTTTTTACTTTGTTTT+TGG + chr5.1:6817331-6817350 MS.gene54215:intron 10.0%
!! AATTCTATTCAAAAAAATGA+AGG - chr5.1:6817806-6817825 None:intergenic 15.0%
!! ATTATACAAAATTATGAGAT+TGG + chr5.1:6819245-6819264 MS.gene54215:intron 15.0%
!! ATTATGAATACTATAATGAA+AGG - chr5.1:6815879-6815898 None:intergenic 15.0%
!! GTTCAAAATAAATAAATTGT+TGG + chr5.1:6818494-6818513 MS.gene54215:intron 15.0%
!! TTTAAGCTAGAATTAAAATT+AGG + chr5.1:6818197-6818216 MS.gene54215:intron 15.0%
!!! ATTTTGTTCTAATACTTTTT+TGG + chr5.1:6819463-6819482 MS.gene54215:intron 15.0%
!!! ATTTTTTTTCTTTTCTATCA+GGG + chr5.1:6819420-6819439 MS.gene54215:intron 15.0%
!!! TAAATCATTATTTTCATTCA+TGG - chr5.1:6818181-6818200 None:intergenic 15.0%
!!! TCAATTTGATTTGATTTTAT+TGG + chr5.1:6815819-6815838 MS.gene54215:intron 15.0%
!! AAAAGATATAAAACAGAGTT+TGG - chr5.1:6815923-6815942 None:intergenic 20.0%
!! AATAAATAAATTGTTGGGTT+TGG + chr5.1:6818500-6818519 MS.gene54215:intron 20.0%
!! ATTATACCTTAGAATATGTT+TGG + chr5.1:6817694-6817713 MS.gene54215:intron 20.0%
!! ATTTAATATTAAGGTAACCT+GGG + chr5.1:6819113-6819132 MS.gene54215:intron 20.0%
!! CATTATAGTATTCATAATGT+TGG + chr5.1:6815881-6815900 MS.gene54215:intron 20.0%
!! GAAAATGTTATTCAAAAAGA+GGG - chr5.1:6815857-6815876 None:intergenic 20.0%
!! GAATTAAAATTAGGAACTAT+TGG + chr5.1:6818206-6818225 MS.gene54215:intron 20.0%
!! GAGAATTATTAAATTGTGAT+CGG + chr5.1:6818328-6818347 MS.gene54215:intron 20.0%
!! GATAATTTACTGAATACATA+TGG - chr5.1:6819203-6819222 None:intergenic 20.0%
!! TGGTAAAGATAATTACAAAT+AGG + chr5.1:6818226-6818245 MS.gene54215:intron 20.0%
!!! AAACATATTCTAAGGTATAA+TGG - chr5.1:6817695-6817714 None:intergenic 20.0%
!!! CATTTTTTTTCTTTTCTATC+AGG + chr5.1:6819419-6819438 MS.gene54215:intron 20.0%
!!! TACATCAGTACTTATATTTT+TGG + chr5.1:6817282-6817301 MS.gene54215:intron 20.0%
!!! TGATTTTATTCTTTTCATAC+AGG + chr5.1:6816938-6816957 MS.gene54215:intron 20.0%
!!! TTGACAGTTAAATTCTAAAA+TGG + chr5.1:6818755-6818774 MS.gene54215:intron 20.0%
!!! TTGTATAATTCACTTCATTT+TGG - chr5.1:6819234-6819253 None:intergenic 20.0%
! AACATCCTAATTTCTTAGTA+TGG - chr5.1:6818733-6818752 None:intergenic 25.0%
! AACCTAATTAAACACTTGTT+TGG + chr5.1:6818273-6818292 MS.gene54215:intron 25.0%
! ATGCATGTAAAACATCTTTA+GGG - chr5.1:6818913-6818932 None:intergenic 25.0%
! ATGTCATTATTGTTCTTGTT+GGG + chr5.1:6818121-6818140 MS.gene54215:CDS 25.0%
! CAAAGCTGGATTTAATATTA+AGG + chr5.1:6819104-6819123 MS.gene54215:CDS 25.0%
! CATGAAATGAAACTAGATTA+AGG - chr5.1:6818851-6818870 None:intergenic 25.0%
! CTAAGAATAACTTATGAAAC+AGG - chr5.1:6816871-6816890 None:intergenic 25.0%
! CTTAATATTAAATCCAGCTT+TGG - chr5.1:6819106-6819125 None:intergenic 25.0%
! GATAAACTAAAAATAGCTCA+TGG + chr5.1:6816834-6816853 MS.gene54215:intron 25.0%
! GATTTAATATTAAGGTAACC+TGG + chr5.1:6819112-6819131 MS.gene54215:intron 25.0%
! GGAAAATGTTATTCAAAAAG+AGG - chr5.1:6815858-6815877 None:intergenic 25.0%
! TAAACTTAAAGTCTTCATCT+GGG + chr5.1:6819577-6819596 MS.gene54215:CDS 25.0%
! TATGCATGTAAAACATCTTT+AGG - chr5.1:6818914-6818933 None:intergenic 25.0%
! TGAAATAATGTTAAGTTTGC+AGG - chr5.1:6817645-6817664 None:intergenic 25.0%
! TGCAACATCATATACAATAA+CGG - chr5.1:6817464-6817483 None:intergenic 25.0%
! TGTAAGTACAATACAATACT+AGG - chr5.1:6817518-6817537 None:intergenic 25.0%
!! AGTAAAATTGTGCATTTTTC+AGG - chr5.1:6815999-6816018 None:intergenic 25.0%
!! ATTAAGTTGTTTATGGAAAC+AGG - chr5.1:6816701-6816720 None:intergenic 25.0%
!! GTGCTTAATTAAGTTGTTTA+TGG - chr5.1:6816708-6816727 None:intergenic 25.0%
!! TATAATTCACTTCATTTTGG+TGG - chr5.1:6819231-6819250 None:intergenic 25.0%
!!! CAAAGATTTTAACAGACATT+AGG - chr5.1:6817999-6818018 None:intergenic 25.0%
!!! TTTACTTTGTTTTTGGATTC+TGG + chr5.1:6817338-6817357 MS.gene54215:intron 25.0%
ACAATACAATACTAGGTGAA+AGG - chr5.1:6817511-6817530 None:intergenic 30.0%
ACTGAATACATATGGAAAGA+GGG - chr5.1:6819195-6819214 None:intergenic 30.0%
AGCCAAACAAGTGTTTAATT+AGG - chr5.1:6818278-6818297 None:intergenic 30.0%
AGGGTAATTGGATAATATAC+AGG - chr5.1:6818426-6818445 None:intergenic 30.0%
ATCATATACAATAACGGCTA+CGG - chr5.1:6817458-6817477 None:intergenic 30.0%
ATGTTGCAAGTAAGTTGTTT+GGG + chr5.1:6817476-6817495 MS.gene54215:intron 30.0%
ATTCATATGTCAGATGTCAA+GGG - chr5.1:6818445-6818464 None:intergenic 30.0%
CGAATCTGAACTTATTAAAC+TGG - chr5.1:6816229-6816248 None:intergenic 30.0%
CTTTCCTAAATACAACAAAG+TGG + chr5.1:6818067-6818086 MS.gene54215:CDS 30.0%
GAATATTGAAGATACCTGAT+AGG - chr5.1:6815786-6815805 None:intergenic 30.0%
GATGTCATTATTGTTCTTGT+TGG + chr5.1:6818120-6818139 MS.gene54215:CDS 30.0%
GTAAACTTAAAGTCTTCATC+TGG + chr5.1:6819576-6819595 MS.gene54215:CDS 30.0%
GTTACAGCTTAAACTAGATA+TGG + chr5.1:6818686-6818705 MS.gene54215:intron 30.0%
GTTTAATTAGGTTCTTATGC+AGG - chr5.1:6818266-6818285 None:intergenic 30.0%
TACCTTAGAATATGTTTGGA+TGG + chr5.1:6817698-6817717 MS.gene54215:intron 30.0%
TACTGAATACATATGGAAAG+AGG - chr5.1:6819196-6819215 None:intergenic 30.0%
TATTCATATGTCAGATGTCA+AGG - chr5.1:6818446-6818465 None:intergenic 30.0%
TCATTCATTCACACTATAGA+AGG - chr5.1:6817968-6817987 None:intergenic 30.0%
TTAAACTAGATATGGCTAGT+TGG + chr5.1:6818694-6818713 MS.gene54215:intron 30.0%
TTAGAATATGTTTGGATGGA+GGG + chr5.1:6817702-6817721 MS.gene54215:intron 30.0%
TTAGGATATGTTTAGGAGAT+GGG - chr5.1:6817841-6817860 None:intergenic 30.0%
! ATCCTTCATTTTGTTCTGAT+AGG + chr5.1:6817374-6817393 MS.gene54215:intron 30.0%
! ATTGGGCCGATATTTAAAAT+CGG + chr5.1:6816644-6816663 MS.gene54215:intron 30.0%
! CAACTCAGTGAAATAGATTT+TGG - chr5.1:6816112-6816131 None:intergenic 30.0%
! CAATCCACTTTGTTGTATTT+AGG - chr5.1:6818074-6818093 None:intergenic 30.0%
! CCATATAAGATTTGAAATCG+CGG + chr5.1:6815961-6815980 MS.gene54215:intron 30.0%
! CTGCTAATTTAGCTTCTTAT+TGG + chr5.1:6817597-6817616 MS.gene54215:intron 30.0%
! GTACTTACACACAATCTTTT+TGG + chr5.1:6817529-6817548 MS.gene54215:intron 30.0%
! TCCTTCATTTTGTTCTGATA+GGG + chr5.1:6817375-6817394 MS.gene54215:intron 30.0%
!! AACTGCTTGCTGTATATTTA+TGG + chr5.1:6818936-6818955 MS.gene54215:intron 30.0%
!! GGATTCTGTTTCTACTTATT+TGG + chr5.1:6819266-6819285 MS.gene54215:intron 30.0%
!! TCTTATTTCCTTTTCTACCA+AGG + chr5.1:6818551-6818570 MS.gene54215:intron 30.0%
!! TTACACACAATCTTTTTGGT+TGG + chr5.1:6817533-6817552 MS.gene54215:intron 30.0%
!!! ACTTTTTTGGACATCTTTAC+AGG + chr5.1:6819476-6819495 MS.gene54215:intron 30.0%
AAACTGATCTCGTTGAAAAG+AGG + chr5.1:6818148-6818167 MS.gene54215:CDS 35.0%
AACAACTTATATGTGTGCAG+TGG - chr5.1:6818477-6818496 None:intergenic 35.0%
AAGATTCAAAACCACCACAT+TGG - chr5.1:6816296-6816315 None:intergenic 35.0%
AAGTACAAACTGGTGTTCTT+GGG + chr5.1:6815698-6815717 MS.gene54215:CDS 35.0%
AGTAGAGAGTCGATAATAAG+AGG - chr5.1:6819293-6819312 None:intergenic 35.0%
AGTGTTTAACTAATCCCACT+TGG + chr5.1:6817115-6817134 MS.gene54215:intron 35.0%
ATATGGAAAGAGGGAGAAAA+GGG - chr5.1:6819186-6819205 None:intergenic 35.0%
ATCATTATGCTCCTTTCCTT+AGG - chr5.1:6817859-6817878 None:intergenic 35.0%
ATCTAACCTGATCAGATGAA+TGG + chr5.1:6816267-6816286 MS.gene54215:intron 35.0%
ATGATGCAGAAGAATGATCA+TGG - chr5.1:6819402-6819421 None:intergenic 35.0%
ATGGAGTACATGTGAAAAGA+TGG - chr5.1:6818640-6818659 None:intergenic 35.0%
ATTAGGTTCTTATGCAGGTA+TGG - chr5.1:6818261-6818280 None:intergenic 35.0%
ATTCTCAGCAAAAACAGTTG+TGG - chr5.1:6818314-6818333 None:intergenic 35.0%
CAATCTCAAACTCAATCAAG+TGG + chr5.1:6819606-6819625 MS.gene54215:CDS 35.0%
CATCTCCTAAACATATCCTA+AGG + chr5.1:6817840-6817859 MS.gene54215:intron 35.0%
CCGCGATTTCAAATCTTATA+TGG - chr5.1:6815964-6815983 None:intergenic 35.0%
CCTAAACATATCCTAAGGAA+AGG + chr5.1:6817845-6817864 MS.gene54215:intron 35.0%
CCTTTCCTTAGGATATGTTT+AGG - chr5.1:6817848-6817867 None:intergenic 35.0%
CTCACCATGATAGGTTAAAA+AGG - chr5.1:6816806-6816825 None:intergenic 35.0%
CTCCATCCAAACATATTCTA+AGG - chr5.1:6817703-6817722 None:intergenic 35.0%
CTTAGAATATGTTTGGATGG+AGG + chr5.1:6817701-6817720 MS.gene54215:intron 35.0%
CTTAGGATATGTTTAGGAGA+TGG - chr5.1:6817842-6817861 None:intergenic 35.0%
GATGTTGCAAGTAAGTTGTT+TGG + chr5.1:6817475-6817494 MS.gene54215:intron 35.0%
GGAGACCATACTAAGAAATT+AGG + chr5.1:6818725-6818744 MS.gene54215:intron 35.0%
GGAGATTGAATGCTAAATCA+AGG - chr5.1:6816061-6816080 None:intergenic 35.0%
GTTTAAGCTGTAACCAAGAA+CGG - chr5.1:6818680-6818699 None:intergenic 35.0%
TAAATTCGACAACACCTATC+AGG + chr5.1:6815769-6815788 MS.gene54215:CDS 35.0%
TAGAAGACAGTCTTGATCAT+CGG + chr5.1:6818818-6818837 MS.gene54215:intron 35.0%
TCCCTATCAGAACAAAATGA+AGG - chr5.1:6817379-6817398 None:intergenic 35.0%
TCTGATAAACCTGTTCTTGT+TGG + chr5.1:6818031-6818050 MS.gene54215:intron 35.0%
TGTGGCAATTCAGTTTGAAT+TGG + chr5.1:6816562-6816581 MS.gene54215:intron 35.0%
TGTTGCAAGTAAGTTGTTTG+GGG + chr5.1:6817477-6817496 MS.gene54215:intron 35.0%
TTAACTAATCCCACTTGGTT+TGG + chr5.1:6817120-6817139 MS.gene54215:intron 35.0%
TTCTGCAAAACAAGAAGACT+TGG + chr5.1:6819548-6819567 MS.gene54215:CDS 35.0%
TTGAATCGAATCGTACCTTT+GGG + chr5.1:6816408-6816427 MS.gene54215:intron 35.0%
TTGTATTTAGGAAAGTCTGC+CGG - chr5.1:6818062-6818081 None:intergenic 35.0%
! ACACTTGTTAACTTCAGTAC+AGG - chr5.1:6817100-6817119 None:intergenic 35.0%
! AGCAGCAATTTTCCGAAAAA+GGG - chr5.1:6819502-6819521 None:intergenic 35.0%
! AGCTCCTTTTTAACCTATCA+TGG + chr5.1:6816799-6816818 MS.gene54215:intron 35.0%
! ATCATGGATTGAGACTGAAT+TGG - chr5.1:6819386-6819405 None:intergenic 35.0%
! CTAAATTAGCCTACCCAAAT+TGG - chr5.1:6817221-6817240 None:intergenic 35.0%
! CTTTCTGCACAAATTCGTTT+TGG + chr5.1:6816135-6816154 MS.gene54215:intron 35.0%
! CTTTTCATACAGGCAACAAT+CGG + chr5.1:6816948-6816967 MS.gene54215:intron 35.0%
! GATGGAGAAAACATGCATTT+TGG - chr5.1:6818622-6818641 None:intergenic 35.0%
! GTAATGTTGAGCGAGATTTT+AGG + chr5.1:6816525-6816544 MS.gene54215:intron 35.0%
! TAAATTGTTGGGTTTGGAGT+TGG + chr5.1:6818506-6818525 MS.gene54215:intron 35.0%
! TTTTGAATCTTTGTGACCTC+TGG + chr5.1:6816305-6816324 MS.gene54215:intron 35.0%
! TTTTGGCATACAATCTCCAA+GGG - chr5.1:6816095-6816114 None:intergenic 35.0%
!! ATGGTTTGTTGTAGAGTAGA+GGG - chr5.1:6817918-6817937 None:intergenic 35.0%
!! ATTTTAGTGACATGAGCAGT+CGG - chr5.1:6816367-6816386 None:intergenic 35.0%
!! ATTTTGGCATACAATCTCCA+AGG - chr5.1:6816096-6816115 None:intergenic 35.0%
!! TACAACAAAGTGGATTGAAG+AGG + chr5.1:6818077-6818096 MS.gene54215:CDS 35.0%
AAAATTGCTGCTGCATTACC+TGG + chr5.1:6819510-6819529 MS.gene54215:CDS 40.0%
AACCTATCATGGTGAGCTTA+TGG + chr5.1:6816810-6816829 MS.gene54215:intron 40.0%
AACTTCAGTACAGGATACTG+AGG - chr5.1:6817091-6817110 None:intergenic 40.0%
AAGGCAAGATTCTCTAGCAT+GGG + chr5.1:6818570-6818589 MS.gene54215:intron 40.0%
AATTGTGATCGGAATCGCTT+TGG + chr5.1:6818339-6818358 MS.gene54215:intron 40.0%
ACTGCAGATGTATGGAAAGA+GGG - chr5.1:6819150-6819169 None:intergenic 40.0%
AGAGTCCCCTTTATATAGTG+AGG + chr5.1:6816439-6816458 MS.gene54215:intron 40.0%
ATAGAAGGAGTGATCGTGAA+TGG - chr5.1:6817953-6817972 None:intergenic 40.0%
CATATGGAAAGAGGGAGAAA+AGG - chr5.1:6819187-6819206 None:intergenic 40.0%
CTATGACAGGATTCAGGATT+AGG - chr5.1:6818801-6818820 None:intergenic 40.0%
CTGCTCATGTCACTAAAATG+CGG + chr5.1:6816368-6816387 MS.gene54215:intron 40.0%
CTTAGTATGGTCTCCAAGAT+AGG - chr5.1:6818720-6818739 None:intergenic 40.0%
GAAGTACAAACTGGTGTTCT+TGG + chr5.1:6815697-6815716 MS.gene54215:CDS 40.0%
GAATCTTGCCTTGGTAGAAA+AGG - chr5.1:6818562-6818581 None:intergenic 40.0%
GTCACTAAAATGCGGAGAAA+GGG + chr5.1:6816376-6816395 MS.gene54215:intron 40.0%
GTTGAATCGAATCGTACCTT+TGG + chr5.1:6816407-6816426 MS.gene54215:intron 40.0%
TAAAATGGTGCATTGCATGC+AGG + chr5.1:6818770-6818789 MS.gene54215:intron 40.0%
TAAACCTGTTCTTGTTGGAC+AGG + chr5.1:6818036-6818055 MS.gene54215:intron 40.0%
TACACTATGCAGTGTTTGCA+TGG + chr5.1:6816188-6816207 MS.gene54215:intron 40.0%
TACTGCAGATGTATGGAAAG+AGG - chr5.1:6819151-6819170 None:intergenic 40.0%
TCAAGACTGTCTTCTATGAC+AGG - chr5.1:6818814-6818833 None:intergenic 40.0%
TCACATGTACTCCATTTAGC+CGG + chr5.1:6818645-6818664 MS.gene54215:intron 40.0%
TCACTAAAATGCGGAGAAAG+GGG + chr5.1:6816377-6816396 MS.gene54215:intron 40.0%
TCTAGATTGAAGAACACTGC+TGG - chr5.1:6819341-6819360 None:intergenic 40.0%
TGAATCGAATCGTACCTTTG+GGG + chr5.1:6816409-6816428 MS.gene54215:intron 40.0%
TGGAAACAGGATACTTGTTC+CGG - chr5.1:6816688-6816707 None:intergenic 40.0%
TGTCACTAAAATGCGGAGAA+AGG + chr5.1:6816375-6816394 MS.gene54215:intron 40.0%
TGTCAGATGTCAAGGGTAAT+TGG - chr5.1:6818438-6818457 None:intergenic 40.0%
TGTCTTCTATGACAGGATTC+AGG - chr5.1:6818807-6818826 None:intergenic 40.0%
! AATCTTTGTGACCTCTGGAA+CGG + chr5.1:6816310-6816329 MS.gene54215:intron 40.0%
! CAGCAGCAATTTTCCGAAAA+AGG - chr5.1:6819503-6819522 None:intergenic 40.0%
! CATCTTTACAGGCCCTTTTT+CGG + chr5.1:6819487-6819506 MS.gene54215:intron 40.0%
! TACAGGAGAAAAGAGTGCAA+TGG - chr5.1:6818409-6818428 None:intergenic 40.0%
! TTTGTTGTAGAGTAGAGGGT+TGG - chr5.1:6817914-6817933 None:intergenic 40.0%
!! CATGGTTTGTTGTAGAGTAG+AGG - chr5.1:6817919-6817938 None:intergenic 40.0%
!! GTCGGGCCGATTTTAAATAT+CGG - chr5.1:6816653-6816672 None:intergenic 40.0%
!!! GATGATGCTGGTTTTACCAA+CGG - chr5.1:6815732-6815751 None:intergenic 40.0%
!!! TTGCACTTGCTGATTTTCCT+TGG + chr5.1:6818377-6818396 MS.gene54215:intron 40.0%
ACCAACAAGCCAAACCAAGT+GGG - chr5.1:6817132-6817151 None:intergenic 45.0%
AGACAAGTCTCCATAGAGGA+CGG + chr5.1:6819033-6819052 MS.gene54215:intron 45.0%
AGTGATCGTGAATGGAGCTT+CGG - chr5.1:6817945-6817964 None:intergenic 45.0%
ATATCGGCCCAATGCAACAT+TGG - chr5.1:6816637-6816656 None:intergenic 45.0%
ATGTCACTGCAAATCGCATC+AGG - chr5.1:6816032-6816051 None:intergenic 45.0%
ATTCTGTGAGCTGTTGCAGT+AGG + chr5.1:6817666-6817685 MS.gene54215:intron 45.0%
ATTGAAACTAGCGCCAAAGC+TGG + chr5.1:6819090-6819109 MS.gene54215:CDS 45.0%
CAAGGCAAGATTCTCTAGCA+TGG + chr5.1:6818569-6818588 MS.gene54215:intron 45.0%
CACATGTACTCCATTTAGCC+GGG + chr5.1:6818646-6818665 MS.gene54215:intron 45.0%
CACTAAAATGCGGAGAAAGG+GGG + chr5.1:6816378-6816397 MS.gene54215:intron 45.0%
CATGCTAGAGAATCTTGCCT+TGG - chr5.1:6818571-6818590 None:intergenic 45.0%
CGCCTCCTCACTATATAAAG+GGG - chr5.1:6816447-6816466 None:intergenic 45.0%
CTCCATAAGCTCACCATGAT+AGG - chr5.1:6816815-6816834 None:intergenic 45.0%
CTGTAACCAAGAACGGAAAC+AGG - chr5.1:6818673-6818692 None:intergenic 45.0%
GAACTGTTCGACTACAGTTG+TGG + chr5.1:6817009-6817028 MS.gene54215:CDS 45.0%
GAAGACAGTGTTTCCATTCC+AGG - chr5.1:6819531-6819550 None:intergenic 45.0%
GAGCGATTACTGCAGATGTA+TGG - chr5.1:6819158-6819177 None:intergenic 45.0%
GAGTGCAATGGAAAGAACCA+AGG - chr5.1:6818397-6818416 None:intergenic 45.0%
GATGAGTCTCTGATGTAGCT+TGG - chr5.1:6817436-6817455 None:intergenic 45.0%
GATTCTCTAGCATGGGATGT+AGG + chr5.1:6818577-6818596 MS.gene54215:intron 45.0%
GCGCCTCCTCACTATATAAA+GGG - chr5.1:6816448-6816467 None:intergenic 45.0%
GCTCAACATTACGATCTCTG+CGG - chr5.1:6816517-6816536 None:intergenic 45.0%
GTCCCCTTTATATAGTGAGG+AGG + chr5.1:6816442-6816461 MS.gene54215:intron 45.0%
TATCGGCCCAATGCAACATT+GGG - chr5.1:6816636-6816655 None:intergenic 45.0%
TATGTTTGGATGGAGGGTCT+TGG + chr5.1:6817708-6817727 MS.gene54215:intron 45.0%
TCTCCAAAGTGAATCACCCT+TGG + chr5.1:6816076-6816095 MS.gene54215:intron 45.0%
TCTCCAAGGGTGATTCACTT+TGG - chr5.1:6816082-6816101 None:intergenic 45.0%
TGCGCCTCCTCACTATATAA+AGG - chr5.1:6816449-6816468 None:intergenic 45.0%
TGTTCTGATAGGGATACAGC+TGG + chr5.1:6817385-6817404 MS.gene54215:intron 45.0%
! AGCTTTGGCGAAGTACAAAC+TGG + chr5.1:6815688-6815707 MS.gene54215:CDS 45.0%
! ATTCTCCCTGGTGCCAATTT+GGG + chr5.1:6817205-6817224 MS.gene54215:intron 45.0%
! TATGGCTAGTTGGCCTATCT+TGG + chr5.1:6818704-6818723 MS.gene54215:intron 45.0%
!! CGAGATTTTAGGAGCAACGT+GGG + chr5.1:6816536-6816555 MS.gene54215:intron 45.0%
!! TTGGTTTGGCTTGTTGGTGT+TGG + chr5.1:6817134-6817153 MS.gene54215:intron 45.0%
!!! TGCTGGTTTTACCAACGGAT+TGG - chr5.1:6815727-6815746 None:intergenic 45.0%
!!! TATTTTTCTATTTTAAATTA+AGG + chr5.1:6817751-6817770 MS.gene54215:intron 5.0%
ACAAGTCTCCATAGAGGACG+GGG + chr5.1:6819035-6819054 MS.gene54215:CDS 50.0%
ACTCTTTGTACACCCCTGTG+AGG - chr5.1:6816607-6816626 None:intergenic 50.0%
ACTGTGCCTCTCAACGGTTA+TGG + chr5.1:6816489-6816508 MS.gene54215:intron 50.0%
AGAGGGAAAAAAGTGAGCCC+AGG - chr5.1:6819133-6819152 None:intergenic 50.0%
ATGAATGGCGTCCAATGTGG+TGG + chr5.1:6816282-6816301 MS.gene54215:intron 50.0%
ATGCGGAGAAAGGGGGTTTA+TGG + chr5.1:6816385-6816404 MS.gene54215:intron 50.0%
CACCAACAAGCCAAACCAAG+TGG - chr5.1:6817133-6817152 None:intergenic 50.0%
CAGATGAATGGCGTCCAATG+TGG + chr5.1:6816279-6816298 MS.gene54215:intron 50.0%
CTCTTTGTACACCCCTGTGA+GGG - chr5.1:6816606-6816625 None:intergenic 50.0%
CTGCAGACAAGTCTCCATAG+AGG + chr5.1:6819029-6819048 MS.gene54215:intron 50.0%
GAATCGAACCTGAGACCTTG+AGG - chr5.1:6817184-6817203 None:intergenic 50.0%
GACAAGTCTCCATAGAGGAC+GGG + chr5.1:6819034-6819053 MS.gene54215:intron 50.0%
GGACTCTTCACAATCCCCAA+AGG - chr5.1:6816426-6816445 None:intergenic 50.0%
GGATGGCCCAATGTTGCATT+GGG + chr5.1:6816627-6816646 MS.gene54215:intron 50.0%
TACTTCGCCAAAGCTGACAC+TGG - chr5.1:6815683-6815702 None:intergenic 50.0%
TGATAGGGATACAGCTGGAC+AGG + chr5.1:6817390-6817409 MS.gene54215:intron 50.0%
TGATTGTTGCGACCCTCACA+GGG + chr5.1:6816591-6816610 MS.gene54215:intron 50.0%
TGCTGCTGCATTACCTGGAA+TGG + chr5.1:6819515-6819534 MS.gene54215:CDS 50.0%
TGGACGCCATTCATCTGATC+AGG - chr5.1:6816276-6816295 None:intergenic 50.0%
TGGATGGCCCAATGTTGCAT+TGG + chr5.1:6816626-6816645 MS.gene54215:intron 50.0%
TGTGAGGGTCGCAACAATCA+GGG - chr5.1:6816591-6816610 None:intergenic 50.0%
TTCTTGGGTGACCAATCCGT+TGG + chr5.1:6815713-6815732 MS.gene54215:CDS 50.0%
TTTGGATGGAGGGTCTTGGT+GGG + chr5.1:6817712-6817731 MS.gene54215:intron 50.0%
! AGGGGTGTACAAAGAGTGGA+TGG + chr5.1:6816610-6816629 MS.gene54215:intron 50.0%
! AGGTTCGATTCGATTCTCCC+TGG + chr5.1:6817193-6817212 MS.gene54215:intron 50.0%
! CATGAAGCGAGTGATGATGC+TGG - chr5.1:6815744-6815763 None:intergenic 50.0%
! GATTCTCCCTGGTGCCAATT+TGG + chr5.1:6817204-6817223 MS.gene54215:intron 50.0%
! GCACAGTGTGCCACTAACAA+CGG - chr5.1:6816476-6816495 None:intergenic 50.0%
! TCACAGGGGTGTACAAAGAG+TGG + chr5.1:6816606-6816625 MS.gene54215:intron 50.0%
! TGAAGAGGTGCGAACAGAGA+GGG + chr5.1:6818092-6818111 MS.gene54215:CDS 50.0%
! TTGAAGAGGTGCGAACAGAG+AGG + chr5.1:6818091-6818110 MS.gene54215:CDS 50.0%
! TTTTGCTTCCCCGTCCTCTA+TGG - chr5.1:6819046-6819065 None:intergenic 50.0%
!! GCGAGATTTTAGGAGCAACG+TGG + chr5.1:6816535-6816554 MS.gene54215:intron 50.0%
!! TCCCACTTGGTTTGGCTTGT+TGG + chr5.1:6817128-6817147 MS.gene54215:intron 50.0%
!! TGAATGGAGCTTCGGTGTCA+TGG - chr5.1:6817937-6817956 None:intergenic 50.0%
!! TGGCTTGTTGGTGTTGGCTT+GGG + chr5.1:6817140-6817159 MS.gene54215:intron 50.0%
!! TTGGCTTGTTGGTGTTGGCT+TGG + chr5.1:6817139-6817158 MS.gene54215:intron 50.0%
AATCGGCCCGACCATTTGCT+CGG + chr5.1:6816661-6816680 MS.gene54215:intron 55.0%
AGCCTACCCAAATTGGCACC+AGG - chr5.1:6817214-6817233 None:intergenic 55.0%
CCAAGAACGGAAACAGGACC+CGG - chr5.1:6818667-6818686 None:intergenic 55.0%
CCTGTTCTTGTTGGACAGGC+CGG + chr5.1:6818040-6818059 MS.gene54215:intron 55.0%
CTGATTGTTGCGACCCTCAC+AGG + chr5.1:6816590-6816609 MS.gene54215:intron 55.0%
CTGTGAGGGTCGCAACAATC+AGG - chr5.1:6816592-6816611 None:intergenic 55.0%
CTTGTTCCGGACCGAGCAAA+TGG - chr5.1:6816675-6816694 None:intergenic 55.0%
GATTGTTGCGACCCTCACAG+GGG + chr5.1:6816592-6816611 MS.gene54215:intron 55.0%
GCCTACCCAAATTGGCACCA+GGG - chr5.1:6817213-6817232 None:intergenic 55.0%
GCGGATCCATAACCGTTGAG+AGG - chr5.1:6816498-6816517 None:intergenic 55.0%
GGAAACAGGACCCGGCTAAA+TGG - chr5.1:6818659-6818678 None:intergenic 55.0%
GTTTGGATGGAGGGTCTTGG+TGG + chr5.1:6817711-6817730 MS.gene54215:intron 55.0%
TAGGAGCAACGTGGGTGATG+TGG + chr5.1:6816544-6816563 MS.gene54215:intron 55.0%
TCGAACCTGAGACCTTGAGG+AGG - chr5.1:6817181-6817200 None:intergenic 55.0%
TGGCACACTGTGCCTCTCAA+CGG + chr5.1:6816483-6816502 MS.gene54215:intron 55.0%
TTCCGGACCGAGCAAATGGT+CGG - chr5.1:6816671-6816690 None:intergenic 55.0%
TTGGAGTGTGCTCCTCCTCA+AGG + chr5.1:6817166-6817185 MS.gene54215:intron 55.0%
TTGGATGGAGGGTCTTGGTG+GGG + chr5.1:6817713-6817732 MS.gene54215:intron 55.0%
! GAAGAGGTGCGAACAGAGAG+GGG + chr5.1:6818093-6818112 MS.gene54215:CDS 55.0%
! TCCCTGGTGCCAATTTGGGT+AGG + chr5.1:6817209-6817228 MS.gene54215:intron 55.0%
! TCGGAGTGTCTGCACTATGC+AGG - chr5.1:6816348-6816367 None:intergenic 55.0%
! TTGAGGAGGAGCACACTCCA+AGG - chr5.1:6817167-6817186 None:intergenic 55.0%
!! CTTTGTGACCTCTGGAACGG+CGG + chr5.1:6816313-6816332 MS.gene54215:intron 55.0%
!! TTGGTGTTGGCTTGGGACCT+TGG + chr5.1:6817147-6817166 MS.gene54215:intron 55.0%
!! TTTGTGACCTCTGGAACGGC+GGG + chr5.1:6816314-6816333 MS.gene54215:intron 55.0%
CCGGCCTGTCCAACAAGAAC+AGG - chr5.1:6818043-6818062 None:intergenic 60.0%
CCGGGTCCTGTTTCCGTTCT+TGG + chr5.1:6818664-6818683 MS.gene54215:intron 60.0%
GGCGCAAGAGCCGTTGTTAG+TGG + chr5.1:6816463-6816482 MS.gene54215:intron 60.0%
GTGCTCCTCCTCAAGGTCTC+AGG + chr5.1:6817173-6817192 MS.gene54215:intron 60.0%
TCCGGACCGAGCAAATGGTC+GGG - chr5.1:6816670-6816689 None:intergenic 60.0%
TCTTGGTGGGGAGAGGAGGA+AGG + chr5.1:6817725-6817744 MS.gene54215:intron 60.0%
!! TTGTGACCTCTGGAACGGCG+GGG + chr5.1:6816315-6816334 MS.gene54215:intron 60.0%
AGGGTCTTGGTGGGGAGAGG+AGG + chr5.1:6817721-6817740 MS.gene54215:intron 65.0%
GCCCGACCATTTGCTCGGTC+CGG + chr5.1:6816666-6816685 MS.gene54215:intron 65.0%
TGGAGGGTCTTGGTGGGGAG+AGG + chr5.1:6817718-6817737 MS.gene54215:intron 65.0%
!! TGTGACCTCTGGAACGGCGG+GGG + chr5.1:6816316-6816335 MS.gene54215:intron 65.0%
ACACACCCCCGCCGTTCCAG+AGG - chr5.1:6816324-6816343 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 6815674 6819641 6815674 ID=MS.gene54215
chr5.1 mRNA 6815674 6819641 6815674 ID=MS.gene54215.t1;Parent=MS.gene54215
chr5.1 exon 6815674 6815790 6815674 ID=MS.gene54215.t1.exon1;Parent=MS.gene54215.t1
chr5.1 CDS 6815674 6815790 6815674 ID=cds.MS.gene54215.t1;Parent=MS.gene54215.t1
chr5.1 exon 6816960 6817030 6816960 ID=MS.gene54215.t1.exon2;Parent=MS.gene54215.t1
chr5.1 CDS 6816960 6817030 6816960 ID=cds.MS.gene54215.t1;Parent=MS.gene54215.t1
chr5.1 exon 6817396 6817484 6817396 ID=MS.gene54215.t1.exon3;Parent=MS.gene54215.t1
chr5.1 CDS 6817396 6817484 6817396 ID=cds.MS.gene54215.t1;Parent=MS.gene54215.t1
chr5.1 exon 6818058 6818169 6818058 ID=MS.gene54215.t1.exon4;Parent=MS.gene54215.t1
chr5.1 CDS 6818058 6818169 6818058 ID=cds.MS.gene54215.t1;Parent=MS.gene54215.t1
chr5.1 exon 6819035 6819125 6819035 ID=MS.gene54215.t1.exon5;Parent=MS.gene54215.t1
chr5.1 CDS 6819035 6819125 6819035 ID=cds.MS.gene54215.t1;Parent=MS.gene54215.t1
chr5.1 exon 6819498 6819641 6819498 ID=MS.gene54215.t1.exon6;Parent=MS.gene54215.t1
chr5.1 CDS 6819498 6819641 6819498 ID=cds.MS.gene54215.t1;Parent=MS.gene54215.t1
Gene Sequence

>MS.gene54215

ATGGCGCCAGTGTCAGCTTTGGCGAAGTACAAACTGGTGTTCTTGGGTGACCAATCCGTTGGTAAAACCAGCATCATCACTCGCTTCATGTACGATAAATTCGACAACACCTATCAGGTATCTTCAATATTCATTCATTCATTGCTCAATTTGATTTGATTTTATTGGATTCACTTGCGTCCCTCTTTTTGAATAACATTTTCCTTTCATTATAGTATTCATAATGTTGGATTTGACTCTACTTGACCAAACTCTGTTTTATATCTTTTATTCTCTTATAATTTGATCCATATAAGATTTGAAATCGCGGTCGTGTTATGATCCTGAAAAATGCACAATTTTACTGTTAAATACGCCTGATGCGATTTGCAGTGACATCAAATACCTTGATTTAGCATTCAATCTCCAAAGTGAATCACCCTTGGAGATTGTATGCCAAAATCTATTTCACTGAGTTGTTGCTTTCTGCACAAATTCGTTTTGGATTTTTTTTTTTTTTTTTTAGTATTATGTGTACACTATGCAGTGTTTGCATGGTATGCTTGTAATAATCCAGTTTAATAAGTTCAGATTCGAATTACTTTTTAGTATTGATCTAACCTGATCAGATGAATGGCGTCCAATGTGGTGGTTTTGAATCTTTGTGACCTCTGGAACGGCGGGGGTGTGTACCTGCATAGTGCAGACACTCCGACTGCTCATGTCACTAAAATGCGGAGAAAGGGGGTTTATGGTTGAATCGAATCGTACCTTTGGGGATTGTGAAGAGTCCCCTTTATATAGTGAGGAGGCGCAAGAGCCGTTGTTAGTGGCACACTGTGCCTCTCAACGGTTATGGATCCGCAGAGATCGTAATGTTGAGCGAGATTTTAGGAGCAACGTGGGTGATGTGGCAATTCAGTTTGAATTGGTCTCCCTGATTGTTGCGACCCTCACAGGGGTGTACAAAGAGTGGATGGCCCAATGTTGCATTGGGCCGATATTTAAAATCGGCCCGACCATTTGCTCGGTCCGGAACAAGTATCCTGTTTCCATAAACAACTTAATTAAGCACTTATCATATAAATACTTAAGTCTAACAAAAGTTAAAATCAAGTCAAACTGTTTTCATGTAAGTTATAAATAAGCTCCTTTTTAACCTATCATGGTGAGCTTATGGAGATAAACTAAAAATAGCTCATGGAAATGTTATAACCTGTTTCATAAGTTATTCTTAGCAAACATACAAATGCTTATATCAGTAGATAAGCTCCTTAACTTATTATGATTTTATTCTTTTCATACAGGCAACAATCGGTATTGATTTTCTGTCAAAAACTATGTATCTTGAAGATCGAACTGTTCGACTACAGTTGTGGTAAGAATTAATATACTAGATCAACATCTCGTACTATAAACATGATAGCAAAATATTCCTCAGTATCCTGTACTGAAGTTAACAAGTGTTTAACTAATCCCACTTGGTTTGGCTTGTTGGTGTTGGCTTGGGACCTTGGAGTGTGCTCCTCCTCAAGGTCTCAGGTTCGATTCGATTCTCCCTGGTGCCAATTTGGGTAGGCTAATTTAGCTTCTTCAAAAAAAGTGTTTATCTAATAAACATTTAGTTTCTACATCAGTACTTATATTTTTGGTGCTGAAGTTTATTTTTTTAATATTTTTATTTTTTTACTTTGTTTTTGGATTCTGGTTTTGAACTGTTCATCCTTCATTTTGTTCTGATAGGGATACAGCTGGACAGGAAAGATTTAGAAGTCTTATTCCAAGCTACATCAGAGACTCATCCGTAGCCGTTATTGTATATGATGTTGCAAGTAAGTTGTTTGGGGCTTGTTTTCCTTTCACCTAGTATTGTATTGTACTTACACACAATCTTTTTGGTTGGACACATCTTTTGCTTCTTAGCAGTTGTTAATCTGATCGAAGCTGCTAATTTAGCTTCTTATTGGTTTTTAACATTTTGATAGTAAACCTGCAAACTTAACATTATTTCAAATTCTGTGAGCTGTTGCAGTAGGATGCCATTATACCTTAGAATATGTTTGGATGGAGGGTCTTGGTGGGGAGAGGAGGAAGGACTTATTTTTCTATTTTAAATTAAGGAAGCTACAAAATGTTTCTAAGATGTTACTCCTTCATTTTTTTGAATAGAATTAGCTCTGCAAAACCCATCTCCTAAACATATCCTAAGGAAAGGAGCATAATGATAAAGTGCATTAGCTCTTTAACTCTGTTTATAACCAACCCTCTACTCTACAACAAACCATGACACCGAAGCTCCATTCACGATCACTCCTTCTATAGTGTGAATGAATGATGCATTGTCCTAATGTCTGTTAAAATCTTTGTTAATAACACTTTCTGATAAACCTGTTCTTGTTGGACAGGCCGGCAGACTTTCCTAAATACAACAAAGTGGATTGAAGAGGTGCGAACAGAGAGGGGCAGTGATGTCATTATTGTTCTTGTTGGGAACAAAACTGATCTCGTTGAAAAGAGGTAATGTGCCATGAATGAAAATAATGATTTAAGCTAGAATTAAAATTAGGAACTATTGGTAAAGATAATTACAAATAGGAATTATTAACCATACCTGCATAAGAACCTAATTAAACACTTGTTTGGCTAGTTTTTCTGAAACCACAACTGTTTTTGCTGAGAATTATTAAATTGTGATCGGAATCGCTTTGGAAATTTTCAGACTGATTGCACTTGCTGATTTTCCTTGGTTCTTTCCATTGCACTCTTTTCTCCTGTATATTATCCAATTACCCTTGACATCTGACATATGAATAAGTACTTGCCACTGCACACATATAAGTTGTTCAAAATAAATAAATTGTTGGGTTTGGAGTTGGAAAACTACTTTGAATTGATAATTCTTATTTCCTTTTCTACCAAGGCAAGATTCTCTAGCATGGGATGTAGGCATGCTTACTACTTGACTGCCAAAATGCATGTTTTCTCCATCTTTTCACATGTACTCCATTTAGCCGGGTCCTGTTTCCGTTCTTGGTTACAGCTTAAACTAGATATGGCTAGTTGGCCTATCTTGGAGACCATACTAAGAAATTAGGATGTTGCTTGACAGTTAAATTCTAAAATGGTGCATTGCATGCAGGTTGATCCTAATCCTGAATCCTGTCATAGAAGACAGTCTTGATCATCGGTGAAAAGCCTTAATCTAGTTTCATTTCATGTTTGAAAGAGTTCTCAGTTTTTTTTCTGTGCTAACTTGACCCTAAAGATGTTTTACATGCATAGTAACTGCTTGCTGTATATTTATGGAGATGTGTGCTATTGTTACTAACAATCTGAAACTAACTGTTCAGTTTTACTTAATTTTCTTTGTGCGAGTCTGCAGACAAGTCTCCATAGAGGACGGGGAAGCAAAAGCACGTGAGCTCAATGTCATGTTTATTGAAACTAGCGCCAAAGCTGGATTTAATATTAAGGTAACCTGGGCTCACTTTTTTCCCTCTTTCCATACATCTGCAGTAATCGCTCTGTTTCCCTTTTCTCCCTCTTTCCATATGTATTCAGTAAATTATCTGTTTCCACCAAAATGAAGTGAATTATACAAAATTATGAGATTGGATTCTGTTTCTACTTATTTGGACCTCTTATTATCGACTCTCTACTTGTTATAGTTTTAGTGATTTTCACTCCAGCAGTGTTCTTCAATCTAGAAAATTCATTTTGCTTGCATTTGCCAATTCAGTCTCAATCCATGATCATTCTTCTGCATCATTTTTTTTCTTTTCTATCAGGGAGATAACTAATGTCAATCTTATTTTGTTCTAATACTTTTTTGGACATCTTTACAGGCCCTTTTTCGGAAAATTGCTGCTGCATTACCTGGAATGGAAACACTGTCTTCTGCAAAACAAGAAGACTTGGTTGATGTAAACTTAAAGTCTTCATCTGGGAATGCTCAATCTCAAACTCAATCAAGTGGATGTTCTTGTTGA
Protein sequence

>MS.gene54215.t1

MAPVSALAKYKLVFLGDQSVGKTSIITRFMYDKFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSSVAVIVYDVASRQTFLNTTKWIEEVRTERGSDVIIVLVGNKTDLVEKRQVSIEDGEAKARELNVMFIETSAKAGFNIKALFRKIAAALPGMETLSSAKQEDLVDVNLKSSSGNAQSQTQSSGCSC