AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060343


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCCGAAGAATACTCCTTTC+AGG 0.283433 8.3:+12141685 MS.gene060343:CDS
GATCATCTCCCTTGCAAATA+AGG 0.299752 8.3:-12141649 None:intergenic
CTTGAATTCTTGAATGACTA+AGG 0.393185 8.3:-12141625 None:intergenic
CTCCTGAAAGGAGTATTCTT+CGG 0.405596 8.3:-12141687 None:intergenic
TCCTGAAAGGAGTATTCTTC+GGG 0.415421 8.3:-12141686 None:intergenic
ACGGTCCCCGGTCTAATTAC+CGG 0.446231 8.3:+12141471 MS.gene060343:CDS
CGCCAGCTGTTGTAGTCCAC+AGG 0.447034 8.3:-12141431 None:intergenic
AATTCAAGACCTTATTTGCA+AGG 0.500490 8.3:+12141640 MS.gene060343:CDS
CTGAGAAGATAACATCGGTC+TGG 0.501527 8.3:-12141502 None:intergenic
GCCACGCCGACCGTGTGAAA+CGG 0.512900 8.3:+12141594 MS.gene060343:CDS
CATCGGTCTGGCGCGAATGC+CGG 0.524753 8.3:-12141490 None:intergenic
CGAATGCCGGTAATTAGACC+GGG 0.532797 8.3:-12141477 None:intergenic
GCGAATGCCGGTAATTAGAC+CGG 0.534546 8.3:-12141478 None:intergenic
ATTCAAGACCTTATTTGCAA+GGG 0.538163 8.3:+12141641 MS.gene060343:CDS
CAGCCCATGTCTACGGTCCC+CGG 0.543615 8.3:+12141459 MS.gene060343:CDS
CGAGCGACAGCCCATGTCTA+CGG 0.544052 8.3:+12141452 MS.gene060343:CDS
TCCCTGTGGACTACAACAGC+TGG 0.568994 8.3:+12141429 MS.gene060343:CDS
TTATCTTCTCAGACGCGGGA+TGG 0.582193 8.3:+12141513 MS.gene060343:CDS
GATGTTATCTTCTCAGACGC+GGG 0.583396 8.3:+12141509 MS.gene060343:CDS
TAGACCGGGGACCGTAGACA+TGG 0.590389 8.3:-12141463 None:intergenic
GGAAAGACCTCCGTTTCACA+CGG 0.611357 8.3:-12141604 None:intergenic
AGACCTCCGTTTCACACGGT+CGG 0.622233 8.3:-12141600 None:intergenic
TCCGTTTCACACGGTCGGCG+TGG 0.626436 8.3:-12141595 None:intergenic
CGATGTTATCTTCTCAGACG+CGG 0.644839 8.3:+12141508 MS.gene060343:CDS
GTATTCATTTGAGTTCCCTG+TGG 0.649334 8.3:+12141415 MS.gene060343:CDS
ACGCCGACCGTGTGAAACGG+AGG 0.662932 8.3:+12141597 MS.gene060343:CDS
AGACCGGGGACCGTAGACAT+GGG 0.678926 8.3:-12141462 None:intergenic
GCCAGCTGTTGTAGTCCACA+GGG 0.679316 8.3:-12141430 None:intergenic
CGCGTCTGAGAAGATAACAT+CGG 0.723208 8.3:-12141507 None:intergenic
GAATGCCGGTAATTAGACCG+GGG 0.739409 8.3:-12141476 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CTTGAATTCTTGAATGACTA+AGG - chr8.3:12141628-12141647 None:intergenic 30.0%
! AATTCAAGACCTTATTTGCA+AGG + chr8.3:12141640-12141659 MS.gene060343:CDS 30.0%
! AGGAGTTGATTAAAAAGATG+GGG + chr8.3:12141705-12141724 MS.gene060343:CDS 30.0%
! ATTCAAGACCTTATTTGCAA+GGG + chr8.3:12141641-12141660 MS.gene060343:CDS 30.0%
! CAGGAGTTGATTAAAAAGAT+GGG + chr8.3:12141704-12141723 MS.gene060343:CDS 30.0%
! CTTTTTAATCAACTCCTGAA+AGG - chr8.3:12141702-12141721 None:intergenic 30.0%
! TCAGGAGTTGATTAAAAAGA+TGG + chr8.3:12141703-12141722 MS.gene060343:CDS 30.0%
! GGCATTGTAGTTTTTGCTTT+CGG + chr8.3:12141534-12141553 MS.gene060343:CDS 35.0%
GATCATCTCCCTTGCAAATA+AGG - chr8.3:12141652-12141671 None:intergenic 40.0%
! GTATTCATTTGAGTTCCCTG+TGG + chr8.3:12141415-12141434 MS.gene060343:CDS 40.0%
!! CTCCTGAAAGGAGTATTCTT+CGG - chr8.3:12141690-12141709 None:intergenic 40.0%
!! TCCTGAAAGGAGTATTCTTC+GGG - chr8.3:12141689-12141708 None:intergenic 40.0%
CGATGTTATCTTCTCAGACG+CGG + chr8.3:12141508-12141527 MS.gene060343:CDS 45.0%
CGCGTCTGAGAAGATAACAT+CGG - chr8.3:12141510-12141529 None:intergenic 45.0%
CTGAGAAGATAACATCGGTC+TGG - chr8.3:12141505-12141524 None:intergenic 45.0%
GATGTTATCTTCTCAGACGC+GGG + chr8.3:12141509-12141528 MS.gene060343:CDS 45.0%
CGAATGCCGGTAATTAGACC+GGG - chr8.3:12141480-12141499 None:intergenic 50.0%
GAATGCCGGTAATTAGACCG+GGG - chr8.3:12141479-12141498 None:intergenic 50.0%
GCCCGAAGAATACTCCTTTC+AGG + chr8.3:12141685-12141704 MS.gene060343:CDS 50.0%
GCGAATGCCGGTAATTAGAC+CGG - chr8.3:12141481-12141500 None:intergenic 50.0%
GGAAAGACCTCCGTTTCACA+CGG - chr8.3:12141607-12141626 None:intergenic 50.0%
TTATCTTCTCAGACGCGGGA+TGG + chr8.3:12141513-12141532 MS.gene060343:CDS 50.0%
ACGGTCCCCGGTCTAATTAC+CGG + chr8.3:12141471-12141490 MS.gene060343:CDS 55.0%
AGACCTCCGTTTCACACGGT+CGG - chr8.3:12141603-12141622 None:intergenic 55.0%
GCCAGCTGTTGTAGTCCACA+GGG - chr8.3:12141433-12141452 None:intergenic 55.0%
! TCCCTGTGGACTACAACAGC+TGG + chr8.3:12141429-12141448 MS.gene060343:CDS 55.0%
AGACCGGGGACCGTAGACAT+GGG - chr8.3:12141465-12141484 None:intergenic 60.0%
CGAGCGACAGCCCATGTCTA+CGG + chr8.3:12141452-12141471 MS.gene060343:CDS 60.0%
CGCCAGCTGTTGTAGTCCAC+AGG - chr8.3:12141434-12141453 None:intergenic 60.0%
TAGACCGGGGACCGTAGACA+TGG - chr8.3:12141466-12141485 None:intergenic 60.0%
ACGCCGACCGTGTGAAACGG+AGG + chr8.3:12141597-12141616 MS.gene060343:CDS 65.0%
CAGCCCATGTCTACGGTCCC+CGG + chr8.3:12141459-12141478 MS.gene060343:CDS 65.0%
CATCGGTCTGGCGCGAATGC+CGG - chr8.3:12141493-12141512 None:intergenic 65.0%
GCCACGCCGACCGTGTGAAA+CGG + chr8.3:12141594-12141613 MS.gene060343:CDS 65.0%
TCCGTTTCACACGGTCGGCG+TGG - chr8.3:12141598-12141617 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 12141406 12141737 12141406 ID=MS.gene060343
chr8.3 mRNA 12141406 12141737 12141406 ID=MS.gene060343.t1;Parent=MS.gene060343
chr8.3 exon 12141406 12141737 12141406 ID=MS.gene060343.t1.exon1;Parent=MS.gene060343.t1
chr8.3 CDS 12141406 12141737 12141406 ID=cds.MS.gene060343.t1;Parent=MS.gene060343.t1
Gene Sequence

>MS.gene060343

AAACTACATGTATTCATTTGAGTTCCCTGTGGACTACAACAGCTGGCGAGCGACAGCCCATGTCTACGGTCCCCGGTCTAATTACCGGCATTCGCGCCAGACCGATGTTATCTTCTCAGACGCGGGATGGCATTGTAGTTTTTGCTTTCGGTATATTTCAGAGTTTGTTTTCAAAATGACTGCTTATAGCCACGCCGACCGTGTGAAACGGAGGTCTTTCCTTAGTCATTCAAGAATTCAAGACCTTATTTGCAAGGGAGATGATCTTTTTGATATGCTGCCCGAAGAATACTCCTTTCAGGAGTTGATTAAAAAGATGGGGTCAATACCTC

Protein sequence

>MS.gene060343.t1

KLHVFIUVPCGLQQLASDSPCLRSPVULPAFAPDRCYLLRRGMALUFLLSVYFRVCFQNDCLUPRRPCETEVFPUSFKNSRPYLQGRUSFUYAARRILLSGVDUKDGVNT