AlfalfaGEDB | MS.gene41119

M. sativa cultivar XinJiangDaYe / MS.gene41119

KEGG: K03872
GO: GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0019538 GO:0019941 GO:0043170 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0051603 GO:0071704 GO:1901564 GO:1901575
PFAM: PF03931.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41119.t1	XP_013456162.1	100	98	0	0	1	98	1	98	1.90E-48	201.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41119.t1	Q2KII4	52.2	92	42	1	7	96	19	110	4.1e-20	98.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41119.t1	I3SR24	100.0	98	0	0	1	98	1	98	1.4e-48	201.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene41119	0.800271	1.56E-48	-1.69E-46
MS.gene049254	MS.gene41119	0.831189	1.92E-55	-1.69E-46
MS.gene049669	MS.gene41119	0.81959	1.07E-52	-1.69E-46
MS.gene050303	MS.gene41119	0.826095	3.27E-54	-1.69E-46
MS.gene050442	MS.gene41119	0.8109	9.16E-51	-1.69E-46
MS.gene050719	MS.gene41119	0.804393	2.21E-49	-1.69E-46
MS.gene050721	MS.gene41119	0.806289	8.84E-50	-1.69E-46
MS.gene051243	MS.gene41119	0.808318	3.29E-50	-1.69E-46
MS.gene051452	MS.gene41119	0.811902	5.55E-51	-1.69E-46
MS.gene051744	MS.gene41119	0.800552	1.37E-48	-1.69E-46
MS.gene051757	MS.gene41119	0.805287	1.44E-49	-1.69E-46
MS.gene051975	MS.gene41119	0.815726	7.97E-52	-1.69E-46
MS.gene052012	MS.gene41119	0.81898	1.48E-52	-1.69E-46
MS.gene052108	MS.gene41119	0.805013	1.64E-49	-1.69E-46
MS.gene052176	MS.gene41119	0.809399	1.93E-50	-1.69E-46
MS.gene052426	MS.gene41119	0.814332	1.63E-51	-1.69E-46
MS.gene052500	MS.gene41119	0.817005	4.13E-52	-1.69E-46
MS.gene052502	MS.gene41119	0.812422	4.27E-51	-1.69E-46
MS.gene052826	MS.gene41119	0.812298	4.55E-51	-1.69E-46
MS.gene052954	MS.gene41119	0.833977	3.91E-56	-1.69E-46
MS.gene053006	MS.gene41119	0.836448	9.30E-57	-1.69E-46
MS.gene053226	MS.gene41119	0.806207	9.20E-50	-1.69E-46
MS.gene053340	MS.gene41119	0.800263	1.56E-48	-1.69E-46
MS.gene05415	MS.gene41119	0.800439	1.44E-48	-1.69E-46
MS.gene054479	MS.gene41119	0.808978	2.37E-50	-1.69E-46
MS.gene054657	MS.gene41119	0.820447	6.83E-53	-1.69E-46
MS.gene05510	MS.gene41119	0.844222	8.64E-59	-1.69E-46
MS.gene05517	MS.gene41119	0.821851	3.24E-53	-1.69E-46
MS.gene055623	MS.gene41119	0.974335	5.65E-138	-1.69E-46
MS.gene055701	MS.gene41119	0.807899	4.03E-50	-1.69E-46
MS.gene055818	MS.gene41119	0.832988	6.90E-56	-1.69E-46
MS.gene055841	MS.gene41119	0.827973	1.16E-54	-1.69E-46
MS.gene05604	MS.gene41119	0.80107	1.07E-48	-1.69E-46
MS.gene056162	MS.gene41119	0.803292	3.74E-49	-1.69E-46
MS.gene056506	MS.gene41119	0.817933	2.55E-52	-1.69E-46
MS.gene056699	MS.gene41119	0.81758	3.06E-52	-1.69E-46
MS.gene056760	MS.gene41119	0.800142	1.66E-48	-1.69E-46
MS.gene056776	MS.gene41119	0.809033	2.31E-50	-1.69E-46
MS.gene056787	MS.gene41119	0.806254	8.99E-50	-1.69E-46
MS.gene056986	MS.gene41119	0.824007	1.02E-53	-1.69E-46
MS.gene057026	MS.gene41119	0.822244	2.63E-53	-1.69E-46
MS.gene057140	MS.gene41119	0.812622	3.86E-51	-1.69E-46
MS.gene057208	MS.gene41119	0.834548	2.81E-56	-1.69E-46
MS.gene057392	MS.gene41119	0.809867	1.53E-50	-1.69E-46
MS.gene057693	MS.gene41119	0.814967	1.18E-51	-1.69E-46
MS.gene05795	MS.gene41119	0.801735	7.83E-49	-1.69E-46
MS.gene058146	MS.gene41119	0.803266	3.78E-49	-1.69E-46
MS.gene058548	MS.gene41119	0.805179	1.51E-49	-1.69E-46
MS.gene058595	MS.gene41119	0.805618	1.22E-49	-1.69E-46
MS.gene059317	MS.gene41119	0.821225	4.52E-53	-1.69E-46
MS.gene059830	MS.gene41119	0.8336	4.86E-56	-1.69E-46
MS.gene060297	MS.gene41119	0.80062	1.32E-48	-1.69E-46
MS.gene060322	MS.gene41119	0.824399	8.24E-54	-1.69E-46
MS.gene060365	MS.gene41119	0.8133	2.74E-51	-1.69E-46
MS.gene060572	MS.gene41119	0.828301	9.70E-55	-1.69E-46
MS.gene060586	MS.gene41119	0.805021	1.63E-49	-1.69E-46
MS.gene060803	MS.gene41119	0.805903	1.07E-49	-1.69E-46
MS.gene060998	MS.gene41119	0.80172	7.88E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41119.t1	MTR_4g064903	100.000	98	0	0	1	98	1	98	7.47e-71	206

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41119.t1	AT5G59140	76.596	94	22	0	5	98	3	96	4.84e-51	155

Find 15 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTCAAGTATGTAGATAAT+TGG	0.305137	4.1:+35983224	None:intergenic
AAAGTTAATTCAGGTTCAAT+AGG	0.336432	4.1:+35983264	None:intergenic
ATTAACTCCAAAGTTAATTC+AGG	0.360632	4.1:+35983255	None:intergenic
CGCAACATGCTTACCTCTCC+AGG	0.383198	4.1:-35984041	MS.gene41119:intron
TATTGAACCTGAATTAACTT+TGG	0.389241	4.1:-35983262	MS.gene41119:CDS
ACAAATCTTCTCAAGAATAG+TGG	0.454935	4.1:+35983844	None:intergenic
ATTAACTTTGGAGTTAATGA+TGG	0.475652	4.1:-35983250	MS.gene41119:CDS
AGAATAGTGGAACTAATCTC+AGG	0.494240	4.1:+35983857	None:intergenic
ATTGGCATCTTGAATTTGCT+AGG	0.521974	4.1:-35983811	MS.gene41119:intron
AACAAAAGAAAAGATGAAGA+AGG	0.540972	4.1:-35984144	None:intergenic
CGTTGACAAAGAAGCTGCCA+TGG	0.556586	4.1:-35984078	MS.gene41119:CDS
AGAGGTAAGCATGTTGCGAA+TGG	0.589014	4.1:+35984046	None:intergenic
GTTAAACTGATCAGTTCCGA+TGG	0.614103	4.1:-35984113	MS.gene41119:CDS
GCGAATGGTTTGTGAAACCA+TGG	0.671918	4.1:+35984061	None:intergenic
AACGACGAATTCGAATCCAT+CGG	0.681941	4.1:+35984097	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAACACTAAAATAAAATTT+AGG	+	chr4.1:35984015-35984034	None:intergenic	10.0%
!!	TAACACTAAAATAAAATTTA+GGG	+	chr4.1:35984014-35984033	None:intergenic	10.0%
!!	AATGTAAACTAAAAATGGTA+AGG	+	chr4.1:35983845-35983864	None:intergenic	20.0%
!!	AATTAGCGTTAATTCATATA+AGG	-	chr4.1:35983405-35983424	MS.gene41119:intron	20.0%
!!!	AGATTTGTCAGTATTTTTAT+TGG	-	chr4.1:35983529-35983548	MS.gene41119:intron	20.0%
!!!	CTAATCGAAAAAGTATAAAA+AGG	+	chr4.1:35983369-35983388	None:intergenic	20.0%
!!!	TAGTTGTAAAAGTTTGTTTT+AGG	-	chr4.1:35983945-35983964	MS.gene41119:intron	20.0%
!!!	TTTTTCGATTAGTTCAATTA+GGG	-	chr4.1:35983377-35983396	MS.gene41119:intron	20.0%
!	ATAAAAAGGTAAAGTGATAG+AGG	+	chr4.1:35983355-35983374	None:intergenic	25.0%
!	ATTAACTCCAAAGTTAATTC+AGG	+	chr4.1:35984106-35984125	None:intergenic	25.0%
!	GGTCAAATGTAAACTAAAAA+TGG	+	chr4.1:35983850-35983869	None:intergenic	25.0%
!	TATTGAACCTGAATTAACTT+TGG	-	chr4.1:35984096-35984115	MS.gene41119:CDS	25.0%
!	TTTCTTGTTAGAATTGCAAT+TGG	-	chr4.1:35983586-35983605	MS.gene41119:intron	25.0%
!!	AAAGTTAATTCAGGTTCAAT+AGG	+	chr4.1:35984097-35984116	None:intergenic	25.0%
!!!	ATTAACTTTGGAGTTAATGA+TGG	-	chr4.1:35984108-35984127	MS.gene41119:CDS	25.0%
!!!	CTTTTTCGATTAGTTCAATT+AGG	-	chr4.1:35983376-35983395	MS.gene41119:intron	25.0%
	ACAAATCTTCTCAAGAATAG+TGG	+	chr4.1:35983517-35983536	None:intergenic	30.0%
	TGAATTGCAATGAGAATGTT+TGG	-	chr4.1:35983663-35983682	MS.gene41119:intron	30.0%
!!	TAGTGTTGATGTGTTTATGT+TGG	-	chr4.1:35984043-35984062	MS.gene41119:CDS	30.0%
!!!	AGATGTGATCTGTTTTTGTT+AGG	-	chr4.1:35983441-35983460	MS.gene41119:intron	30.0%
!!!	GATGTGATCTGTTTTTGTTA+GGG	-	chr4.1:35983442-35983461	MS.gene41119:intron	30.0%
	CTAAAAATGGTAAGGTTGCT+TGG	+	chr4.1:35983837-35983856	None:intergenic	35.0%
	TAGAGGAAGAAAAACAAACC+TGG	+	chr4.1:35983338-35983357	None:intergenic	35.0%
	TTGTAAACAGTGTTCAACTC+CGG	-	chr4.1:35983733-35983752	MS.gene41119:intron	35.0%
!	AGAATAGTGGAACTAATCTC+AGG	+	chr4.1:35983504-35983523	None:intergenic	35.0%
!	ATTGGCATCTTGAATTTGCT+AGG	-	chr4.1:35983547-35983566	MS.gene41119:intron	35.0%
!!!	GTTTATGTTGGTTTTGCTAG+TGG	-	chr4.1:35984055-35984074	MS.gene41119:CDS	35.0%
	AACACTGTTTACAACGCATC+TGG	+	chr4.1:35983727-35983746	None:intergenic	40.0%
	AACGACGAATTCGAATCCAT+CGG	+	chr4.1:35983264-35983283	None:intergenic	40.0%
	ATGTGAATGTTTCCATGCCA+CGG	+	chr4.1:35983886-35983905	None:intergenic	40.0%
	CACAAAAGCAATCACGAGAA+AGG	+	chr4.1:35983924-35983943	None:intergenic	40.0%
	GAAGAAAAACAAACCTGGAG+AGG	+	chr4.1:35983333-35983352	None:intergenic	40.0%
	GTTAAACTGATCAGTTCCGA+TGG	-	chr4.1:35983245-35983264	MS.gene41119:CDS	40.0%
	TGTTAACCTTCGATTCCCAA+GGG	+	chr4.1:35983776-35983795	None:intergenic	40.0%
!	AGTTTTGCTGAAAGGGAACA+CGG	-	chr4.1:35983465-35983484	MS.gene41119:intron	40.0%
!	GTTAGGGAGTTTTGCTGAAA+GGG	-	chr4.1:35983458-35983477	MS.gene41119:intron	40.0%
!	TGTTAGGGAGTTTTGCTGAA+AGG	-	chr4.1:35983457-35983476	MS.gene41119:intron	40.0%
!!	CTTTCTCGTGATTGCTTTTG+TGG	-	chr4.1:35983922-35983941	MS.gene41119:intron	40.0%
!!!	TGTTGGTTTTGCTAGTGGAA+AGG	-	chr4.1:35984060-35984079	MS.gene41119:CDS	40.0%
	AGAGGTAAGCATGTTGCGAA+TGG	+	chr4.1:35983315-35983334	None:intergenic	45.0%
	GTGTTAACCTTCGATTCCCA+AGG	+	chr4.1:35983777-35983796	None:intergenic	45.0%
	TGTTCAACTCCGGATTACCA+GGG	-	chr4.1:35983743-35983762	MS.gene41119:intron	45.0%
!	GCGAATGGTTTGTGAAACCA+TGG	+	chr4.1:35983300-35983319	None:intergenic	45.0%
	CACTTTCCCTTGGGAATCGA+AGG	-	chr4.1:35983767-35983786	MS.gene41119:intron	50.0%
	CGTTGACAAAGAAGCTGCCA+TGG	-	chr4.1:35983280-35983299	MS.gene41119:CDS	50.0%
	CTTCGATTCCCAAGGGAAAG+TGG	+	chr4.1:35983769-35983788	None:intergenic	50.0%
	GTGTTCAACTCCGGATTACC+AGG	-	chr4.1:35983742-35983761	MS.gene41119:intron	50.0%
	CGCAACATGCTTACCTCTCC+AGG	-	chr4.1:35983317-35983336	MS.gene41119:intron	55.0%
	GGAAAGTGGCCCTGGTAATC+CGG	+	chr4.1:35983755-35983774	None:intergenic	55.0%
!	TACCAGGGCCACTTTCCCTT+GGG	-	chr4.1:35983758-35983777	MS.gene41119:intron	55.0%
!	TTACCAGGGCCACTTTCCCT+TGG	-	chr4.1:35983757-35983776	MS.gene41119:intron	55.0%
	TGTTTCCATGCCACGGACGC+AGG	+	chr4.1:35983879-35983898	None:intergenic	60.0%
	TTCCCAAGGGAAAGTGGCCC+TGG	+	chr4.1:35983763-35983782	None:intergenic	60.0%
	ATGCCACGGACGCAGGCTAG+CGG	+	chr4.1:35983872-35983891	None:intergenic	65.0%
	TGCCACGGACGCAGGCTAGC+GGG	+	chr4.1:35983871-35983890	None:intergenic	70.0%
!	GCTAGCCTGCGTCCGTGGCA+TGG	-	chr4.1:35983871-35983890	MS.gene41119:CDS	70.0%
!	GACCCGCTAGCCTGCGTCCG+TGG	-	chr4.1:35983866-35983885	MS.gene41119:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	35983227	35984153	35983227	ID=MS.gene41119
chr4.1	mRNA	35983227	35984153	35983227	ID=MS.gene41119.t1;Parent=MS.gene41119
chr4.1	exon	35984042	35984153	35984042	ID=MS.gene41119.t1.exon1;Parent=MS.gene41119.t1
chr4.1	CDS	35984042	35984153	35984042	ID=cds.MS.gene41119.t1;Parent=MS.gene41119.t1
chr4.1	exon	35983812	35983917	35983812	ID=MS.gene41119.t1.exon2;Parent=MS.gene41119.t1
chr4.1	CDS	35983812	35983917	35983812	ID=cds.MS.gene41119.t1;Parent=MS.gene41119.t1
chr4.1	exon	35983227	35983305	35983227	ID=MS.gene41119.t1.exon3;Parent=MS.gene41119.t1
chr4.1	CDS	35983227	35983305	35983227	ID=cds.MS.gene41119.t1;Parent=MS.gene41119.t1

Gene Sequence

>MS.gene41119

ATGAAGAAGGAAGACACAGTTAAACTGATCAGTTCCGATGGATTCGAATTCGTCGTTGACAAAGAAGCTGCCATGGTTTCACAAACCATTCGCAACATGCTTACCTCTCCAGGTTTGTTTTTCTTCCTCTATCACTTTACCTTTTTATACTTTTTCGATTAGTTCAATTAGGGTTACGAATTAGCGTTAATTCATATAAGGTTAAGATTGTTGAAGATGTGATCTGTTTTTGTTAGGGAGTTTTGCTGAAAGGGAACACGGACAAGTGACTTTTCCTGAGATTAGTTCCACTATTCTTGAGAAGATTTGTCAGTATTTTTATTGGCATCTTGAATTTGCTAGGTATTTCATTTTCTTATTTTCTTGTTAGAATTGCAATTGGAGAAAAATCTGAAATTTATGCACATTCATACACTTTACAATGAAATCTGAAATCTGAATTGCAATGAGAATGTTTGGCTGTTTTAAGCTCAAAAAAAGTTATTGTTTGTGTGAATCCAGATGCGTTGTAAACAGTGTTCAACTCCGGATTACCAGGGCCACTTTCCCTTGGGAATCGAAGGTTAACACAAAATATTGAAAATTATTGAGTGACTTGAAATTATAACCAAGCAACCTTACCATTTTTAGTTTACATTTGACCCGCTAGCCTGCGTCCGTGGCATGGAAACATTCACATCATCATTTTATAATACCTTTCTCGTGATTGCTTTTGTGGTAGTTGTAAAAGTTTGTTTTAGGTATTAGAGTTATGTTTTTCTTACAGAAGTTAAGCTTTTTAAATCCCTAAATTTTATTTTAGTGTTATTTAAATTATAGTGTTGATGTGTTTATGTTGGTTTTGCTAGTGGAAAGGACAGAGAGTTTCCTATTGAACCTGAATTAACTTTGGAGTTAATGATGGCTGCCAATTATCTACATACTTGA

Protein sequence

>MS.gene41119.t1

MKKEDTVKLISSDGFEFVVDKEAAMVSQTIRNMLTSPGSFAEREHGQVTFPEISSTILEKICQYFYWHLEFASGKDREFPIEPELTLELMMAANYLHT