Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47691.t1 | RHN57948.1 | 57 | 200 | 60 | 3 | 1 | 200 | 1 | 174 | 4.30E-38 | 169.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47691.t1 | G7K4I5 | 57.0 | 200 | 60 | 3 | 1 | 200 | 1 | 174 | 3.1e-38 | 169.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene47691 | 0.815398 | 9.44E-52 | -1.69E-46 |
| MS.gene049159 | MS.gene47691 | 0.816118 | 6.52E-52 | -1.69E-46 |
| MS.gene049190 | MS.gene47691 | 0.804845 | 1.78E-49 | -1.69E-46 |
| MS.gene049341 | MS.gene47691 | 0.818202 | 2.22E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene47691 | 0.810365 | 1.20E-50 | -1.69E-46 |
| MS.gene049435 | MS.gene47691 | 0.806353 | 8.57E-50 | -1.69E-46 |
| MS.gene049821 | MS.gene47691 | 0.824356 | 8.44E-54 | -1.69E-46 |
| MS.gene049824 | MS.gene47691 | 0.809272 | 2.05E-50 | -1.69E-46 |
| MS.gene049850 | MS.gene47691 | 0.827264 | 1.72E-54 | -1.69E-46 |
| MS.gene049979 | MS.gene47691 | 0.808063 | 3.72E-50 | -1.69E-46 |
| MS.gene050020 | MS.gene47691 | 0.849105 | 4.00E-60 | -1.69E-46 |
| MS.gene050807 | MS.gene47691 | 0.850629 | 1.50E-60 | -1.69E-46 |
| MS.gene050808 | MS.gene47691 | 0.823379 | 1.43E-53 | -1.69E-46 |
| MS.gene050815 | MS.gene47691 | 0.823761 | 1.16E-53 | -1.69E-46 |
| MS.gene050959 | MS.gene47691 | 0.801025 | 1.09E-48 | -1.69E-46 |
| MS.gene051102 | MS.gene47691 | 0.80095 | 1.13E-48 | -1.69E-46 |
| MS.gene051247 | MS.gene47691 | 0.834519 | 2.86E-56 | -1.69E-46 |
| MS.gene051265 | MS.gene47691 | 0.802825 | 4.67E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene47691 | 0.810707 | 1.01E-50 | -1.69E-46 |
| MS.gene051328 | MS.gene47691 | 0.825349 | 4.92E-54 | -1.69E-46 |
| MS.gene05157 | MS.gene47691 | 0.837182 | 6.04E-57 | -1.69E-46 |
| MS.gene051700 | MS.gene47691 | 0.813623 | 2.33E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene47691 | 0.870616 | 1.28E-66 | -1.69E-46 |
| MS.gene051720 | MS.gene47691 | 0.860644 | 1.79E-63 | -1.69E-46 |
| MS.gene052020 | MS.gene47691 | 0.836911 | 7.09E-57 | -1.69E-46 |
| MS.gene052133 | MS.gene47691 | 0.849102 | 4.01E-60 | -1.69E-46 |
| MS.gene052323 | MS.gene47691 | 0.810639 | 1.04E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene47691 | 0.830632 | 2.63E-55 | -1.69E-46 |
| MS.gene052611 | MS.gene47691 | 0.803889 | 2.81E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene47691 | 0.868079 | 8.52E-66 | -1.69E-46 |
| MS.gene052732 | MS.gene47691 | 0.816326 | 5.86E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene47691 | 0.817479 | 3.23E-52 | -1.69E-46 |
| MS.gene053079 | MS.gene47691 | 0.805604 | 1.23E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene47691 | 0.831101 | 2.02E-55 | -1.69E-46 |
| MS.gene053174 | MS.gene47691 | 0.809894 | 1.51E-50 | -1.69E-46 |
| MS.gene053221 | MS.gene47691 | 0.835418 | 1.70E-56 | -1.69E-46 |
| MS.gene053226 | MS.gene47691 | 0.825839 | 3.77E-54 | -1.69E-46 |
| MS.gene053565 | MS.gene47691 | 0.802827 | 4.66E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene47691 | 0.822363 | 2.47E-53 | -1.69E-46 |
| MS.gene053599 | MS.gene47691 | 0.829147 | 6.06E-55 | -1.69E-46 |
| MS.gene05364 | MS.gene47691 | 0.816224 | 6.17E-52 | -1.69E-46 |
| MS.gene054550 | MS.gene47691 | 0.812519 | 4.07E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene47691 | 0.843881 | 1.07E-58 | -1.69E-46 |
| MS.gene05462 | MS.gene47691 | 0.82217 | 2.73E-53 | -1.69E-46 |
| MS.gene055465 | MS.gene47691 | 0.865983 | 3.97E-65 | -1.69E-46 |
| MS.gene055466 | MS.gene47691 | 0.870119 | 1.86E-66 | -1.69E-46 |
| MS.gene055468 | MS.gene47691 | 0.859837 | 3.14E-63 | -1.69E-46 |
| MS.gene055469 | MS.gene47691 | 0.83114 | 1.98E-55 | -1.69E-46 |
| MS.gene055512 | MS.gene47691 | 0.829183 | 5.94E-55 | -1.69E-46 |
| MS.gene055580 | MS.gene47691 | -0.836628 | 8.37E-57 | -1.69E-46 |
| MS.gene055608 | MS.gene47691 | 0.811131 | 8.16E-51 | -1.69E-46 |
| MS.gene055890 | MS.gene47691 | 0.816908 | 4.34E-52 | -1.69E-46 |
| MS.gene055891 | MS.gene47691 | 0.816372 | 5.72E-52 | -1.69E-46 |
| MS.gene056043 | MS.gene47691 | -0.824594 | 7.42E-54 | -1.69E-46 |
| MS.gene056215 | MS.gene47691 | 0.800821 | 1.20E-48 | -1.69E-46 |
| MS.gene056308 | MS.gene47691 | 0.823125 | 1.64E-53 | -1.69E-46 |
| MS.gene056553 | MS.gene47691 | 0.818979 | 1.48E-52 | -1.69E-46 |
| MS.gene056643 | MS.gene47691 | 0.809182 | 2.15E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene47691 | 0.807452 | 5.02E-50 | -1.69E-46 |
| MS.gene057444 | MS.gene47691 | 0.80904 | 2.30E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene47691 | 0.805122 | 1.55E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene47691 | 0.821392 | 4.14E-53 | -1.69E-46 |
| MS.gene058339 | MS.gene47691 | 0.801857 | 7.39E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene47691 | 0.825508 | 4.51E-54 | -1.69E-46 |
| MS.gene058636 | MS.gene47691 | 0.823901 | 1.08E-53 | -1.69E-46 |
| MS.gene058829 | MS.gene47691 | 0.807969 | 3.90E-50 | -1.69E-46 |
| MS.gene058852 | MS.gene47691 | 0.817814 | 2.71E-52 | -1.69E-46 |
| MS.gene059015 | MS.gene47691 | 0.80805 | 3.75E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene47691 | 0.803817 | 2.91E-49 | -1.69E-46 |
| MS.gene059725 | MS.gene47691 | 0.809391 | 1.94E-50 | -1.69E-46 |
| MS.gene05978 | MS.gene47691 | 0.801405 | 9.15E-49 | -1.69E-46 |
| MS.gene059842 | MS.gene47691 | 0.801054 | 1.08E-48 | -1.69E-46 |
| MS.gene059937 | MS.gene47691 | 0.807848 | 4.14E-50 | -1.69E-46 |
| MS.gene060194 | MS.gene47691 | 0.849757 | 2.63E-60 | -1.69E-46 |
| MS.gene060429 | MS.gene47691 | 0.803987 | 2.68E-49 | -1.69E-46 |
| MS.gene060519 | MS.gene47691 | 0.804968 | 1.67E-49 | -1.69E-46 |
| MS.gene060587 | MS.gene47691 | 0.877816 | 4.64E-69 | -1.69E-46 |
| MS.gene061176 | MS.gene47691 | 0.801817 | 7.53E-49 | -1.69E-46 |
| MS.gene061177 | MS.gene47691 | 0.801368 | 9.30E-49 | -1.69E-46 |
| MS.gene061178 | MS.gene47691 | 0.801099 | 1.06E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47691.t1 | MTR_5g094950 | 50.096 | 521 | 149 | 10 | 1 | 506 | 1 | 425 | 3.03e-123 | 373 |
| MS.gene47691.t1 | MTR_5g095050 | 43.191 | 470 | 182 | 10 | 4 | 456 | 4 | 405 | 5.61e-90 | 287 |
| MS.gene47691.t1 | MTR_5g094970 | 46.032 | 378 | 127 | 6 | 69 | 446 | 5 | 305 | 2.29e-77 | 247 |
| MS.gene47691.t1 | MTR_5g094960 | 45.000 | 260 | 128 | 1 | 257 | 501 | 107 | 366 | 2.92e-52 | 185 |
| MS.gene47691.t1 | MTR_5g094960 | 39.744 | 312 | 91 | 10 | 1 | 299 | 1 | 228 | 7.42e-36 | 140 |
| MS.gene47691.t1 | MTR_5g094920 | 36.538 | 520 | 207 | 16 | 1 | 506 | 1 | 411 | 5.79e-51 | 183 |
| MS.gene47691.t1 | MTR_5g077500 | 45.802 | 262 | 86 | 7 | 210 | 457 | 40 | 259 | 3.08e-42 | 152 |
| MS.gene47691.t1 | MTR_5g094990 | 44.737 | 228 | 111 | 1 | 289 | 501 | 133 | 360 | 2.48e-41 | 155 |
| MS.gene47691.t1 | MTR_5g094990 | 38.723 | 235 | 86 | 7 | 1 | 211 | 1 | 201 | 1.76e-23 | 103 |
| MS.gene47691.t1 | MTR_5g094850 | 42.908 | 282 | 93 | 6 | 3 | 271 | 1 | 227 | 9.41e-39 | 148 |
| MS.gene47691.t1 | MTR_5g094850 | 37.838 | 296 | 142 | 5 | 253 | 525 | 114 | 390 | 1.33e-33 | 134 |
| MS.gene47691.t1 | MTR_5g094850 | 42.908 | 282 | 93 | 6 | 3 | 271 | 1 | 227 | 1.08e-38 | 148 |
| MS.gene47691.t1 | MTR_5g094850 | 39.236 | 288 | 120 | 5 | 253 | 502 | 114 | 384 | 3.37e-34 | 135 |
| MS.gene47691.t1 | MTR_5g094910 | 50.000 | 168 | 79 | 1 | 264 | 431 | 79 | 241 | 2.93e-34 | 133 |
| MS.gene47691.t1 | MTR_5g094910 | 31.077 | 325 | 99 | 10 | 1 | 312 | 1 | 213 | 2.16e-13 | 72.4 |
| MS.gene47691.t1 | MTR_5g095370 | 44.516 | 155 | 61 | 3 | 1 | 154 | 1 | 131 | 7.24e-26 | 106 |
| MS.gene47691.t1 | MTR_5g095390 | 41.872 | 203 | 92 | 6 | 3 | 197 | 1 | 185 | 1.56e-23 | 102 |
| MS.gene47691.t1 | MTR_5g095400 | 46.400 | 125 | 54 | 3 | 31 | 154 | 6 | 118 | 7.19e-18 | 87.0 |
| MS.gene47691.t1 | MTR_1g071620 | 40.881 | 159 | 53 | 4 | 323 | 477 | 71 | 192 | 1.47e-17 | 82.0 |
| MS.gene47691.t1 | MTR_5g095310 | 43.333 | 120 | 55 | 3 | 36 | 154 | 2 | 109 | 4.50e-17 | 83.6 |
| MS.gene47691.t1 | MTR_5g095430 | 45.000 | 120 | 53 | 3 | 36 | 154 | 2 | 109 | 7.76e-17 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 82 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATGACCCAGGTTCGATTT+TGG | 0.197878 | 5.3:-72699776 | None:intergenic |
| GAATTAGAGTTGGAAGTTTC+GGG | 0.232160 | 5.3:+72700747 | MS.gene47691:CDS |
| GGAGAAACTTGCTAGGTTAC+AGG | 0.283073 | 5.3:+72700035 | MS.gene47691:CDS |
| TGAATTAGAGTTGGAAGTTT+CGG | 0.309252 | 5.3:+72700746 | MS.gene47691:CDS |
| GGAAACGTTGAACAACAATT+TGG | 0.315230 | 5.3:+72700899 | MS.gene47691:CDS |
| TGAGTTAGAATCTGAGGTTT+TGG | 0.346512 | 5.3:+72700653 | MS.gene47691:CDS |
| TGAACTAGAGCATCGAGTTT+TGG | 0.347114 | 5.3:+72700485 | MS.gene47691:CDS |
| GTTTCAAGAGGAGCTTGAAT+TGG | 0.356825 | 5.3:+72700014 | MS.gene47691:CDS |
| CTTCCTTCAACTTCTTGACT+TGG | 0.368972 | 5.3:-72700341 | None:intergenic |
| CGAGCCTCCTGCTGATACTA+TGG | 0.369008 | 5.3:+72701103 | MS.gene47691:CDS |
| TGAGTTAGAGCTTCAAGTTT+TGG | 0.375044 | 5.3:+72700401 | MS.gene47691:CDS |
| TGACAATGTTGAACGTGTTT+TGG | 0.375700 | 5.3:+72699921 | MS.gene47691:CDS |
| AGTAACACTTCAACGAGATC+TGG | 0.378949 | 5.3:-72699874 | None:intergenic |
| CTGTTTGATTTCGCGTTCAA+CGG | 0.382205 | 5.3:+72701568 | MS.gene47691:CDS |
| TGAGGAAGTTGAAGGATAAG+TGG | 0.396275 | 5.3:+72700679 | MS.gene47691:CDS |
| TTTACAGCACAATGTTGAAA+TGG | 0.412766 | 5.3:+72699981 | MS.gene47691:CDS |
| GGAGAATGATAAAACTGCTT+TGG | 0.414411 | 5.3:+72700845 | MS.gene47691:CDS |
| TTAGCTGATAGAGATACTAT+TGG | 0.415573 | 5.3:+72700285 | MS.gene47691:CDS |
| TCGAAGCAAGGTTCGAGTAT+TGG | 0.418664 | 5.3:+72700824 | MS.gene47691:CDS |
| TTGAAGAAAGTGAAAGTAGC+TGG | 0.420289 | 5.3:+72700132 | MS.gene47691:CDS |
| GAGTCTTTAAACACAAAGTT+CGG | 0.421134 | 5.3:+72700993 | MS.gene47691:CDS |
| AAAGAGGAACATTGAGTTGA+GGG | 0.429427 | 5.3:+72700191 | MS.gene47691:CDS |
| TTTGGAGTTGAGGAAGTTGA+AGG | 0.434593 | 5.3:+72700671 | MS.gene47691:CDS |
| GATTGAAAATGTCAAATTGA+AGG | 0.436713 | 5.3:+72700878 | MS.gene47691:CDS |
| TGAAGAAGTTGAAGGGGAAG+TGG | 0.443788 | 5.3:+72700772 | MS.gene47691:CDS |
| GTCCTCAGGTGCAAAACAAC+GGG | 0.448627 | 5.3:+72701207 | MS.gene47691:CDS |
| AATGATAAAACTGCTTTGGC+TGG | 0.449815 | 5.3:+72700849 | MS.gene47691:CDS |
| TTCTGTAGAGAAAGATAAAG+AGG | 0.476033 | 5.3:+72701133 | MS.gene47691:CDS |
| TCATCTCTAACAAGAATCAC+AGG | 0.478957 | 5.3:-72699811 | None:intergenic |
| CTGTTGATGTTCTGAGAAAG+AGG | 0.481826 | 5.3:+72700175 | MS.gene47691:CDS |
| GAAGCTAAAGGTGCACCATC+AGG | 0.484062 | 5.3:+72701254 | MS.gene47691:CDS |
| TGTGCTGTAAATGTAGAATC+TGG | 0.488371 | 5.3:-72699969 | None:intergenic |
| CAAGCTAGCTGCCAGTCCTC+AGG | 0.495740 | 5.3:+72701193 | MS.gene47691:CDS |
| AACAAGAATCACAGGGTTGG+TGG | 0.496938 | 5.3:-72699803 | None:intergenic |
| ATCAGCTAAACTAGTTATCT+CGG | 0.497069 | 5.3:-72700271 | None:intergenic |
| GAAGAAGTTGAAGGGGAAGT+GGG | 0.503194 | 5.3:+72700773 | MS.gene47691:CDS |
| TCGGGATTGAAGAAGTTGAA+GGG | 0.511191 | 5.3:+72700765 | MS.gene47691:CDS |
| GTCAAGAAGTTGAAGGAAGA+TGG | 0.513911 | 5.3:+72700345 | MS.gene47691:CDS |
| TTCGGGATTGAAGAAGTTGA+AGG | 0.514395 | 5.3:+72700764 | MS.gene47691:CDS |
| AGACGAGAAGCTCAAGGTTG+AGG | 0.521486 | 5.3:+72700962 | MS.gene47691:CDS |
| GCATGAAAGAGTTGCAAGGT+TGG | 0.521848 | 5.3:+72701022 | MS.gene47691:CDS |
| AACGAATGGTGAATTAGAGT+TGG | 0.523751 | 5.3:+72700737 | MS.gene47691:CDS |
| AAAGTGAAAGTAGCTGGGTT+AGG | 0.525794 | 5.3:+72700138 | MS.gene47691:CDS |
| ATTCAACCCAAAATCGAACC+TGG | 0.527062 | 5.3:+72699769 | MS.gene47691:CDS |
| GAATTATGAGTTAGAATCTG+AGG | 0.528199 | 5.3:+72700647 | MS.gene47691:CDS |
| TGTTGATGTGCTTCGAAGCA+AGG | 0.528265 | 5.3:+72700812 | MS.gene47691:CDS |
| ACTTGCTAGGTTACAGGCTG+AGG | 0.533572 | 5.3:+72700041 | MS.gene47691:CDS |
| TTAATGAGTGTAGATACCTC+TGG | 0.537571 | 5.3:+72701062 | MS.gene47691:CDS |
| ATCTACACTCATTAAAACTT+CGG | 0.544129 | 5.3:-72701054 | None:intergenic |
| TTGAATTGGAGAAACTTGCT+AGG | 0.544441 | 5.3:+72700028 | MS.gene47691:CDS |
| AAGTTGTGAAGCTCGGTTGA+AGG | 0.545324 | 5.3:+72700245 | MS.gene47691:CDS |
| GTAACACTTCAACGAGATCT+GGG | 0.548255 | 5.3:-72699873 | None:intergenic |
| TGAGGAAGGTAAAAGAGAGA+AGG | 0.548951 | 5.3:+72700221 | MS.gene47691:CDS |
| TCAACAACCATAGTATCAGC+AGG | 0.558053 | 5.3:-72701110 | None:intergenic |
| CGGGATTGAAGAAGTTGAAG+GGG | 0.558106 | 5.3:+72700766 | MS.gene47691:CDS |
| TTGTTAGAGTCTGAAGCTAA+AGG | 0.569033 | 5.3:+72701242 | MS.gene47691:CDS |
| AGAAGGAAAGTTGTGAAGCT+CGG | 0.573889 | 5.3:+72700238 | MS.gene47691:CDS |
| TTGAGGGAAATGTGTGAGGA+AGG | 0.576476 | 5.3:+72700207 | MS.gene47691:CDS |
| TGTTAGAGATGAAGAAGCTG+TGG | 0.578329 | 5.3:+72699822 | MS.gene47691:CDS |
| GTGCCAAGTCAAGAAGTTGA+AGG | 0.578672 | 5.3:+72700338 | MS.gene47691:CDS |
| TGAAGAAAGTGAAAGTAGCT+GGG | 0.598840 | 5.3:+72700133 | MS.gene47691:CDS |
| CTCTGGTGGTGGAAACGATG+AGG | 0.601316 | 5.3:+72701079 | MS.gene47691:CDS |
| AGTGTAGATACCTCTGGTGG+TGG | 0.602833 | 5.3:+72701068 | MS.gene47691:CDS |
| GGAGAAACAAAAGTTTCAAG+AGG | 0.606040 | 5.3:+72700002 | MS.gene47691:CDS |
| TGGCAACTATAAGTGAGTTG+AGG | 0.607774 | 5.3:+72700919 | MS.gene47691:CDS |
| TCTGGTGGTGGAAACGATGA+GGG | 0.614819 | 5.3:+72701080 | MS.gene47691:CDS |
| AGTCCTCAGGTGCAAAACAA+CGG | 0.617401 | 5.3:+72701206 | MS.gene47691:CDS |
| GAAAGAGGAACATTGAGTTG+AGG | 0.620134 | 5.3:+72700190 | MS.gene47691:CDS |
| TCTAACAAGAATCACAGGGT+TGG | 0.623457 | 5.3:-72699806 | None:intergenic |
| ATTGGCAGACGAGAAGCTCA+AGG | 0.630706 | 5.3:+72700956 | MS.gene47691:CDS |
| TGAAAGAGTTGCAAGGTTGG+AGG | 0.631396 | 5.3:+72701025 | MS.gene47691:CDS |
| AGATGACAACACACTTAATG+TGG | 0.632788 | 5.3:+72700614 | MS.gene47691:CDS |
| TTCAACCCAAAATCGAACCT+GGG | 0.633225 | 5.3:+72699770 | MS.gene47691:CDS |
| AATTGCATGAAAGAGTTGCA+AGG | 0.633271 | 5.3:+72701018 | MS.gene47691:CDS |
| CATCTCTAACAAGAATCACA+GGG | 0.641894 | 5.3:-72699810 | None:intergenic |
| CTCATCGTTTCCACCACCAG+AGG | 0.650565 | 5.3:-72701078 | None:intergenic |
| GTTGCGCTAAACAAAACGAA+TGG | 0.654742 | 5.3:+72700723 | MS.gene47691:CDS |
| TTGGTGGAAGATGATGACCC+AGG | 0.663433 | 5.3:-72699787 | None:intergenic |
| GCAAAACAACGGGAGCAAAG+AGG | 0.667911 | 5.3:+72701217 | MS.gene47691:CDS |
| TGAGTTGAGGGAAATGTGTG+AGG | 0.685982 | 5.3:+72700203 | MS.gene47691:CDS |
| ACAACCATAGTATCAGCAGG+AGG | 0.723572 | 5.3:-72701107 | None:intergenic |
| ATGAGTGTAGATACCTCTGG+TGG | 0.724549 | 5.3:+72701065 | MS.gene47691:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAAAGAAAAACAATGAGT+TGG | + | chr5.3:72700314-72700333 | MS.gene47691:CDS | 25.0% |
| ! | ATCTACACTCATTAAAACTT+CGG | - | chr5.3:72701057-72701076 | None:intergenic | 25.0% |
| ! | GATTGAAAATGTCAAATTGA+AGG | + | chr5.3:72700878-72700897 | MS.gene47691:CDS | 25.0% |
| ! | TAAGAAAGTGAAAAAACTAG+AGG | + | chr5.3:72700092-72700111 | MS.gene47691:CDS | 25.0% |
| ! | TAATATTCAATATGCGTGAT+CGG | + | chr5.3:72701347-72701366 | MS.gene47691:intron | 25.0% |
| !! | TTTAGAGTTGAAGGAAAAAA+GGG | + | chr5.3:72700587-72700606 | MS.gene47691:CDS | 25.0% |
| !! | TTTGGAGTTGAACAAAAAAA+AGG | + | chr5.3:72700503-72700522 | MS.gene47691:CDS | 25.0% |
| !!! | TTTTAGAGTTGAAGGAAAAA+AGG | + | chr5.3:72700586-72700605 | MS.gene47691:CDS | 25.0% |
| AAGTGAAAAAACTAGAGGAA+CGG | + | chr5.3:72700097-72700116 | MS.gene47691:CDS | 30.0% | |
| ATCAGCTAAACTAGTTATCT+CGG | - | chr5.3:72700274-72700293 | None:intergenic | 30.0% | |
| CAGAAAATGCATATCTCTAT+AGG | - | chr5.3:72701309-72701328 | None:intergenic | 30.0% | |
| GAATTATGAGTTAGAATCTG+AGG | + | chr5.3:72700647-72700666 | MS.gene47691:CDS | 30.0% | |
| GAGGAGCAAAAAGATAAAAT+TGG | + | chr5.3:72700938-72700957 | MS.gene47691:CDS | 30.0% | |
| GAGTCTTTAAACACAAAGTT+CGG | + | chr5.3:72700993-72701012 | MS.gene47691:CDS | 30.0% | |
| TAATTGGTTAGAAGATTGCT+AGG | + | chr5.3:72701375-72701394 | MS.gene47691:intron | 30.0% | |
| TACATAATTTCCAAACCTGA+TGG | - | chr5.3:72701272-72701291 | None:intergenic | 30.0% | |
| TGAATTAGAGTTGGAAGTTT+CGG | + | chr5.3:72700746-72700765 | MS.gene47691:CDS | 30.0% | |
| TTAGCTGATAGAGATACTAT+TGG | + | chr5.3:72700285-72700304 | MS.gene47691:CDS | 30.0% | |
| TTCTGTAGAGAAAGATAAAG+AGG | + | chr5.3:72701133-72701152 | MS.gene47691:CDS | 30.0% | |
| TTTACAGCACAATGTTGAAA+TGG | + | chr5.3:72699981-72700000 | MS.gene47691:CDS | 30.0% | |
| ! | ACAATTTTGTTGATGTGCTA+AGG | + | chr5.3:72700451-72700470 | MS.gene47691:CDS | 30.0% |
| !! | ATCAGAATCGATTTTAGACA+TGG | - | chr5.3:72701539-72701558 | None:intergenic | 30.0% |
| !! | TTTGGTGTTGAACGAAAAAA+GGG | + | chr5.3:72700419-72700438 | MS.gene47691:CDS | 30.0% |
| !!! | TTTTGGTGTTGAACGAAAAA+AGG | + | chr5.3:72700418-72700437 | MS.gene47691:CDS | 30.0% |
| AACGAATGGTGAATTAGAGT+TGG | + | chr5.3:72700737-72700756 | MS.gene47691:CDS | 35.0% | |
| AATGATAAAACTGCTTTGGC+TGG | + | chr5.3:72700849-72700868 | MS.gene47691:CDS | 35.0% | |
| AATTGCATGAAAGAGTTGCA+AGG | + | chr5.3:72701018-72701037 | MS.gene47691:CDS | 35.0% | |
| AGATGACAACACACTTAATG+TGG | + | chr5.3:72700614-72700633 | MS.gene47691:CDS | 35.0% | |
| ATGCACGAATTATGTGACTA+TGG | - | chr5.3:72701409-72701428 | None:intergenic | 35.0% | |
| CATCTCTAACAAGAATCACA+GGG | - | chr5.3:72699813-72699832 | None:intergenic | 35.0% | |
| GAATTAGAGTTGGAAGTTTC+GGG | + | chr5.3:72700747-72700766 | MS.gene47691:CDS | 35.0% | |
| GGAAACGTTGAACAACAATT+TGG | + | chr5.3:72700899-72700918 | MS.gene47691:CDS | 35.0% | |
| GGAGAAACAAAAGTTTCAAG+AGG | + | chr5.3:72700002-72700021 | MS.gene47691:CDS | 35.0% | |
| GGAGAATGATAAAACTGCTT+TGG | + | chr5.3:72700845-72700864 | MS.gene47691:CDS | 35.0% | |
| GGAGTTGAACAAAAAAAAGG+TGG | + | chr5.3:72700506-72700525 | MS.gene47691:CDS | 35.0% | |
| TCATCTCTAACAAGAATCAC+AGG | - | chr5.3:72699814-72699833 | None:intergenic | 35.0% | |
| TGAAGAAAGTGAAAGTAGCT+GGG | + | chr5.3:72700133-72700152 | MS.gene47691:CDS | 35.0% | |
| TGCACGAATTATGTGACTAT+GGG | - | chr5.3:72701408-72701427 | None:intergenic | 35.0% | |
| TGCGTGATCGGATAAATAAT+TGG | + | chr5.3:72701359-72701378 | MS.gene47691:intron | 35.0% | |
| TGTGCTGTAAATGTAGAATC+TGG | - | chr5.3:72699972-72699991 | None:intergenic | 35.0% | |
| TTAATGAGTGTAGATACCTC+TGG | + | chr5.3:72701062-72701081 | MS.gene47691:CDS | 35.0% | |
| TTGAAGAAAGTGAAAGTAGC+TGG | + | chr5.3:72700132-72700151 | MS.gene47691:CDS | 35.0% | |
| ! | CGATTTTAGACATGGTTCTT+CGG | - | chr5.3:72701531-72701550 | None:intergenic | 35.0% |
| ! | TGACAATGTTGAACGTGTTT+TGG | + | chr5.3:72699921-72699940 | MS.gene47691:CDS | 35.0% |
| ! | TTGAATTGGAGAAACTTGCT+AGG | + | chr5.3:72700028-72700047 | MS.gene47691:CDS | 35.0% |
| ! | TTGTTAGAGTCTGAAGCTAA+AGG | + | chr5.3:72701242-72701261 | MS.gene47691:CDS | 35.0% |
| !! | AAAGAGGAACATTGAGTTGA+GGG | + | chr5.3:72700191-72700210 | MS.gene47691:CDS | 35.0% |
| !! | TGAGTTAGAATCTGAGGTTT+TGG | + | chr5.3:72700653-72700672 | MS.gene47691:CDS | 35.0% |
| !! | TGAGTTAGAGCTTCAAGTTT+TGG | + | chr5.3:72700401-72700420 | MS.gene47691:CDS | 35.0% |
| !!! | GCATGAAGTTTTAGAGTTGA+AGG | + | chr5.3:72700578-72700597 | MS.gene47691:CDS | 35.0% |
| AAAGTGAAAGTAGCTGGGTT+AGG | + | chr5.3:72700138-72700157 | MS.gene47691:CDS | 40.0% | |
| AGAAGGAAAGTTGTGAAGCT+CGG | + | chr5.3:72700238-72700257 | MS.gene47691:CDS | 40.0% | |
| AGAGTTGAAGGAAAAAAGGG+TGG | + | chr5.3:72700590-72700609 | MS.gene47691:CDS | 40.0% | |
| AGTAACACTTCAACGAGATC+TGG | - | chr5.3:72699877-72699896 | None:intergenic | 40.0% | |
| ATTCAACCCAAAATCGAACC+TGG | + | chr5.3:72699769-72699788 | MS.gene47691:CDS | 40.0% | |
| CTTCCTTCAACTTCTTGACT+TGG | - | chr5.3:72700344-72700363 | None:intergenic | 40.0% | |
| GAGTTGAAGGAAAAAAGGGT+GGG | + | chr5.3:72700591-72700610 | MS.gene47691:CDS | 40.0% | |
| GTAACACTTCAACGAGATCT+GGG | - | chr5.3:72699876-72699895 | None:intergenic | 40.0% | |
| GTCAAGAAGTTGAAGGAAGA+TGG | + | chr5.3:72700345-72700364 | MS.gene47691:CDS | 40.0% | |
| GTTGCGCTAAACAAAACGAA+TGG | + | chr5.3:72700723-72700742 | MS.gene47691:CDS | 40.0% | |
| GTTTCAAGAGGAGCTTGAAT+TGG | + | chr5.3:72700014-72700033 | MS.gene47691:CDS | 40.0% | |
| TCAACAACCATAGTATCAGC+AGG | - | chr5.3:72701113-72701132 | None:intergenic | 40.0% | |
| TCGGGATTGAAGAAGTTGAA+GGG | + | chr5.3:72700765-72700784 | MS.gene47691:CDS | 40.0% | |
| TCTAACAAGAATCACAGGGT+TGG | - | chr5.3:72699809-72699828 | None:intergenic | 40.0% | |
| TGAGGAAGGTAAAAGAGAGA+AGG | + | chr5.3:72700221-72700240 | MS.gene47691:CDS | 40.0% | |
| TGAGGAAGTTGAAGGATAAG+TGG | + | chr5.3:72700679-72700698 | MS.gene47691:CDS | 40.0% | |
| TGGCAACTATAAGTGAGTTG+AGG | + | chr5.3:72700919-72700938 | MS.gene47691:CDS | 40.0% | |
| TGTTAGAGATGAAGAAGCTG+TGG | + | chr5.3:72699822-72699841 | MS.gene47691:CDS | 40.0% | |
| TTCAACCCAAAATCGAACCT+GGG | + | chr5.3:72699770-72699789 | MS.gene47691:CDS | 40.0% | |
| TTCGGGATTGAAGAAGTTGA+AGG | + | chr5.3:72700764-72700783 | MS.gene47691:CDS | 40.0% | |
| ! | CTGTTTGATTTCGCGTTCAA+CGG | + | chr5.3:72701568-72701587 | MS.gene47691:CDS | 40.0% |
| ! | GAAAGAGGAACATTGAGTTG+AGG | + | chr5.3:72700190-72700209 | MS.gene47691:CDS | 40.0% |
| ! | TTTGGAGTTGAGGAAGTTGA+AGG | + | chr5.3:72700671-72700690 | MS.gene47691:CDS | 40.0% |
| !! | CTGTTGATGTTCTGAGAAAG+AGG | + | chr5.3:72700175-72700194 | MS.gene47691:CDS | 40.0% |
| !!! | AATCTGAGGTTTTGGAGTTG+AGG | + | chr5.3:72700661-72700680 | MS.gene47691:CDS | 40.0% |
| !!! | TGAACTAGAGCATCGAGTTT+TGG | + | chr5.3:72700485-72700504 | MS.gene47691:CDS | 40.0% |
| AACAAGAATCACAGGGTTGG+TGG | - | chr5.3:72699806-72699825 | None:intergenic | 45.0% | |
| AAGTTGTGAAGCTCGGTTGA+AGG | + | chr5.3:72700245-72700264 | MS.gene47691:CDS | 45.0% | |
| ACAACCATAGTATCAGCAGG+AGG | - | chr5.3:72701110-72701129 | None:intergenic | 45.0% | |
| AGTCCTCAGGTGCAAAACAA+CGG | + | chr5.3:72701206-72701225 | MS.gene47691:CDS | 45.0% | |
| ATGAGTGTAGATACCTCTGG+TGG | + | chr5.3:72701065-72701084 | MS.gene47691:CDS | 45.0% | |
| CATAATTCGTGCATCATCCC+AGG | + | chr5.3:72701415-72701434 | MS.gene47691:intron | 45.0% | |
| CGGGATTGAAGAAGTTGAAG+GGG | + | chr5.3:72700766-72700785 | MS.gene47691:CDS | 45.0% | |
| GAAGAAGTTGAAGGGGAAGT+GGG | + | chr5.3:72700773-72700792 | MS.gene47691:CDS | 45.0% | |
| GCATGAAAGAGTTGCAAGGT+TGG | + | chr5.3:72701022-72701041 | MS.gene47691:CDS | 45.0% | |
| GTGCCAAGTCAAGAAGTTGA+AGG | + | chr5.3:72700338-72700357 | MS.gene47691:CDS | 45.0% | |
| TCGAAGCAAGGTTCGAGTAT+TGG | + | chr5.3:72700824-72700843 | MS.gene47691:CDS | 45.0% | |
| TGAAAGAGTTGCAAGGTTGG+AGG | + | chr5.3:72701025-72701044 | MS.gene47691:CDS | 45.0% | |
| TGAAGAAGTTGAAGGGGAAG+TGG | + | chr5.3:72700772-72700791 | MS.gene47691:CDS | 45.0% | |
| TGTTGATGTGCTTCGAAGCA+AGG | + | chr5.3:72700812-72700831 | MS.gene47691:CDS | 45.0% | |
| TTCGCGGTTCTTTCACGAAT+TGG | + | chr5.3:72701465-72701484 | MS.gene47691:intron | 45.0% | |
| TTGAGGGAAATGTGTGAGGA+AGG | + | chr5.3:72700207-72700226 | MS.gene47691:CDS | 45.0% | |
| ! | GATGACCCAGGTTCGATTTT+GGG | - | chr5.3:72699778-72699797 | None:intergenic | 45.0% |
| ! | GGTGTTGAACGAAAAAAGGG+TGG | + | chr5.3:72700422-72700441 | MS.gene47691:CDS | 45.0% |
| ! | TAAAGGTGCACCATCAGGTT+TGG | + | chr5.3:72701259-72701278 | MS.gene47691:intron | 45.0% |
| ! | TGAGTTGAGGGAAATGTGTG+AGG | + | chr5.3:72700203-72700222 | MS.gene47691:CDS | 45.0% |
| ! | TGATGACCCAGGTTCGATTT+TGG | - | chr5.3:72699779-72699798 | None:intergenic | 45.0% |
| !! | GGAGAAACTTGCTAGGTTAC+AGG | + | chr5.3:72700035-72700054 | MS.gene47691:CDS | 45.0% |
| AGACGAGAAGCTCAAGGTTG+AGG | + | chr5.3:72700962-72700981 | MS.gene47691:CDS | 50.0% | |
| AGTGTAGATACCTCTGGTGG+TGG | + | chr5.3:72701068-72701087 | MS.gene47691:CDS | 50.0% | |
| ATTGGCAGACGAGAAGCTCA+AGG | + | chr5.3:72700956-72700975 | MS.gene47691:CDS | 50.0% | |
| GCAAAACAACGGGAGCAAAG+AGG | + | chr5.3:72701217-72701236 | MS.gene47691:CDS | 50.0% | |
| GTCCTCAGGTGCAAAACAAC+GGG | + | chr5.3:72701207-72701226 | MS.gene47691:CDS | 50.0% | |
| TTCGTGAAAGAACCGCGAAC+CGG | - | chr5.3:72701464-72701483 | None:intergenic | 50.0% | |
| TTGGTGGAAGATGATGACCC+AGG | - | chr5.3:72699790-72699809 | None:intergenic | 50.0% | |
| ! | ACTTGCTAGGTTACAGGCTG+AGG | + | chr5.3:72700041-72700060 | MS.gene47691:CDS | 50.0% |
| ! | GAAGCTAAAGGTGCACCATC+AGG | + | chr5.3:72701254-72701273 | MS.gene47691:CDS | 50.0% |
| !! | GTTGTTTTGCACCTGAGGAC+TGG | - | chr5.3:72701207-72701226 | None:intergenic | 50.0% |
| !! | TCTGGTGGTGGAAACGATGA+GGG | + | chr5.3:72701080-72701099 | MS.gene47691:CDS | 50.0% |
| ACGAACCAATTCGCGGTTCC+TGG | - | chr5.3:72701436-72701455 | None:intergenic | 55.0% | |
| CGAACCAATTCGCGGTTCCT+GGG | - | chr5.3:72701435-72701454 | None:intergenic | 55.0% | |
| CGAGCCTCCTGCTGATACTA+TGG | + | chr5.3:72701103-72701122 | MS.gene47691:CDS | 55.0% | |
| CTCATCGTTTCCACCACCAG+AGG | - | chr5.3:72701081-72701100 | None:intergenic | 55.0% | |
| GGATCGCACGAACCAATTCG+CGG | - | chr5.3:72701443-72701462 | None:intergenic | 55.0% | |
| TCATCCCAGGAACCGCGAAT+TGG | + | chr5.3:72701428-72701447 | MS.gene47691:intron | 55.0% | |
| !! | CTCCCGTTGTTTTGCACCTG+AGG | - | chr5.3:72701212-72701231 | None:intergenic | 55.0% |
| !! | CTCTGGTGGTGGAAACGATG+AGG | + | chr5.3:72701079-72701098 | MS.gene47691:CDS | 55.0% |
| !! | GCGAATTGGTTCGTGCGATC+CGG | + | chr5.3:72701442-72701461 | MS.gene47691:intron | 55.0% |
| CAAGCTAGCTGCCAGTCCTC+AGG | + | chr5.3:72701193-72701212 | MS.gene47691:CDS | 60.0% | |
| ! | GGTTCGTGCGATCCGGTTCG+CGG | + | chr5.3:72701449-72701468 | MS.gene47691:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 72699763 | 72701615 | 72699763 | ID=MS.gene47691 |
| chr5.3 | mRNA | 72699763 | 72701615 | 72699763 | ID=MS.gene47691.t1;Parent=MS.gene47691 |
| chr5.3 | exon | 72699763 | 72701275 | 72699763 | ID=MS.gene47691.t1.exon1;Parent=MS.gene47691.t1 |
| chr5.3 | CDS | 72699763 | 72701275 | 72699763 | ID=cds.MS.gene47691.t1;Parent=MS.gene47691.t1 |
| chr5.3 | exon | 72701521 | 72701615 | 72701521 | ID=MS.gene47691.t1.exon2;Parent=MS.gene47691.t1 |
| chr5.3 | CDS | 72701521 | 72701615 | 72701521 | ID=cds.MS.gene47691.t1;Parent=MS.gene47691.t1 |
| Gene Sequence |
| Protein sequence |
