Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50493.t1 | XP_013449750.1 | 98 | 307 | 5 | 1 | 1 | 306 | 1 | 307 | 2.10E-175 | 624.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| No data available in table | |||||||||||
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50493.t1 | A0A072U3Q0 | 98.0 | 307 | 5 | 1 | 1 | 306 | 1 | 307 | 1.5e-175 | 624.8 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene50493 | 0.800676 | 1.29E-48 | -1.69E-46 |
| MS.gene049254 | MS.gene50493 | 0.810027 | 1.41E-50 | -1.69E-46 |
| MS.gene049272 | MS.gene50493 | 0.802879 | 4.55E-49 | -1.69E-46 |
| MS.gene049435 | MS.gene50493 | 0.802663 | 5.04E-49 | -1.69E-46 |
| MS.gene049666 | MS.gene50493 | 0.801025 | 1.09E-48 | -1.69E-46 |
| MS.gene049850 | MS.gene50493 | 0.803152 | 3.99E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene50493 | 0.847049 | 1.48E-59 | -1.69E-46 |
| MS.gene050273 | MS.gene50493 | 0.816175 | 6.33E-52 | -1.69E-46 |
| MS.gene050561 | MS.gene50493 | 0.801011 | 1.10E-48 | -1.69E-46 |
| MS.gene051198 | MS.gene50493 | 0.830456 | 2.91E-55 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50493.t1 | MTR_7g095100 | 98.046 | 307 | 5 | 1 | 1 | 306 | 1 | 307 | 0.0 | 613 |
| MS.gene50493.t1 | MTR_1g081260 | 64.610 | 308 | 102 | 3 | 1 | 306 | 1 | 303 | 9.51e-144 | 407 |
| MS.gene50493.t1 | MTR_7g114790 | 62.581 | 310 | 106 | 4 | 1 | 305 | 1 | 305 | 3.60e-139 | 395 |
| MS.gene50493.t1 | MTR_5g069570 | 40.351 | 285 | 153 | 7 | 30 | 306 | 34 | 309 | 1.61e-64 | 206 |
| MS.gene50493.t1 | MTR_3g076610 | 36.396 | 283 | 151 | 5 | 5 | 266 | 8 | 282 | 1.19e-60 | 196 |
| MS.gene50493.t1 | MTR_4g104760 | 38.722 | 266 | 144 | 5 | 5 | 258 | 8 | 266 | 2.30e-60 | 195 |
| MS.gene50493.t1 | MTR_4g055230 | 37.152 | 323 | 165 | 11 | 1 | 304 | 1 | 304 | 1.25e-57 | 187 |
| MS.gene50493.t1 | MTR_3g082430 | 39.241 | 237 | 134 | 5 | 27 | 258 | 13 | 244 | 7.75e-55 | 180 |
| MS.gene50493.t1 | MTR_7g103900 | 36.201 | 279 | 153 | 8 | 1 | 259 | 1 | 274 | 5.77e-52 | 173 |
| MS.gene50493.t1 | MTR_2g084965 | 38.686 | 274 | 142 | 8 | 6 | 259 | 8 | 275 | 4.87e-49 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50493.t1 | AT5G28150 | 60.261 | 307 | 103 | 6 | 1 | 306 | 1 | 289 | 2.40e-120 | 347 |
| MS.gene50493.t1 | AT3G04860 | 57.143 | 308 | 111 | 5 | 1 | 306 | 1 | 289 | 7.90e-120 | 346 |
| MS.gene50493.t1 | AT2G04220 | 42.169 | 249 | 134 | 5 | 14 | 258 | 12 | 254 | 1.82e-63 | 203 |
| MS.gene50493.t1 | AT4G12690 | 41.129 | 248 | 135 | 5 | 15 | 258 | 9 | 249 | 3.88e-62 | 199 |
| MS.gene50493.t1 | AT4G12690 | 41.129 | 248 | 135 | 5 | 15 | 258 | 9 | 249 | 3.88e-62 | 199 |
| MS.gene50493.t1 | AT5G48270 | 37.805 | 328 | 160 | 12 | 5 | 306 | 13 | 322 | 4.29e-56 | 184 |
| MS.gene50493.t1 | AT2G27770 | 32.143 | 336 | 179 | 11 | 1 | 304 | 1 | 319 | 2.18e-48 | 164 |
| MS.gene50493.t1 | AT3G13229 | 34.188 | 234 | 138 | 8 | 33 | 255 | 8 | 236 | 2.21e-37 | 135 |
| MS.gene50493.t1 | AT5G11000 | 32.432 | 259 | 131 | 10 | 28 | 258 | 30 | 272 | 9.29e-35 | 130 |
| MS.gene50493.t1 | AT2G25200 | 25.138 | 362 | 184 | 12 | 6 | 304 | 16 | 353 | 1.99e-30 | 118 |
Find 49 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGAGTTTCCATCTTGTTT+TGG | 0.305139 | 7.3:+16047576 | MS.gene50493:CDS |
| ACAAAGAATTTGATGGGCCA+AGG | 0.577061 | 7.3:+16047729 | MS.gene50493:CDS |
| ACATCCCAAAACACTTCAAC+TGG | 0.400830 | 7.3:-16048272 | None:intergenic |
| ACTAAATAATACCCCTCCAA+TGG | 0.586748 | 7.3:-16047918 | None:intergenic |
| AGTGTTGGAATTGATGAATT+GGG | 0.473318 | 7.3:+16047756 | MS.gene50493:CDS |
| AGTTATGCTAAATTTGGTTC+TGG | 0.191766 | 7.3:+16047888 | MS.gene50493:CDS |
| ATCTATCAATGTAATTTAAG+AGG | 0.448731 | 7.3:+16047678 | MS.gene50493:CDS |
| ATGGGCCAAGGCCTTAGTGT+TGG | 0.505702 | 7.3:+16047741 | MS.gene50493:CDS |
| ATTCATCAATTCCAACACTA+AGG | 0.542567 | 7.3:-16047752 | None:intergenic |
| ATTGAGTGTGATACACTTGT+TGG | 0.493306 | 7.3:+16048140 | MS.gene50493:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTCATCAATTCCAACACTA+AGG | - | chr7.3:16047755-16047774 | None:intergenic | 30.0% | |
| CATGTTTGATTACAGTTTCA+TGG | + | chr7.3:16047706-16047725 | MS.gene50493:CDS | 30.0% | |
| TCAATTCTAATCACAAGACA+AGG | - | chr7.3:16048176-16048195 | None:intergenic | 30.0% | |
| TCATGGACAAAGAATTTGAT+GGG | + | chr7.3:16047723-16047742 | MS.gene50493:CDS | 30.0% | |
| TGTTGTGTTCAACAAAAAGA+TGG | + | chr7.3:16047941-16047960 | MS.gene50493:CDS | 30.0% | |
| TTCATGGACAAAGAATTTGA+TGG | + | chr7.3:16047722-16047741 | MS.gene50493:CDS | 30.0% | |
| ACTAAATAATACCCCTCCAA+TGG | - | chr7.3:16047921-16047940 | None:intergenic | 35.0% | |
| ATTGAGTGTGATACACTTGT+TGG | + | chr7.3:16048140-16048159 | MS.gene50493:CDS | 35.0% | |
| CAAGAAATGTTACTGTGCAA+AGG | + | chr7.3:16048079-16048098 | MS.gene50493:CDS | 35.0% | |
| GATACTGACAACAATGTTAG+TGG | + | chr7.3:16048008-16048027 | MS.gene50493:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 16047573 | 16048493 | 16047573 | ID=MS.gene50493 |
| chr7.3 | mRNA | 16047573 | 16048493 | 16047573 | ID=MS.gene50493.t1;Parent=MS.gene50493 |
| chr7.3 | exon | 16047573 | 16048493 | 16047573 | ID=MS.gene50493.t1.exon1;Parent=MS.gene50493.t1 |
| chr7.3 | CDS | 16047573 | 16048493 | 16047573 | ID=cds.MS.gene50493.t1;Parent=MS.gene50493.t1 |
| Gene Sequence |
>MS.gene50493 ATGAAGGAGTTTCCATCTTGTTTTGGAGAAAATGGTGTTCAGGTTGCAGATTCATCTTCATCCTCTTCAAGTAGCATAAGAGGGCCACAAAATGTGGTTACTTGTATCTATCAATGTAATTTAAGAGGTCGTTCATGTTTGATTACAGTTTCATGGACAAAGAATTTGATGGGCCAAGGCCTTAGTGTTGGAATTGATGAATTGGGAAATAGTTGTTTATGCAAGGTTGATATAAAGCCATGGTTGTTTTCAAAGAGAAAAGGGTGTAAGAATTTGGAAGTTGAATCTAGTAAAGTTGTTATACTTTGGGATTTGAGTTATGCTAAATTTGGTTCTGGGCCAGAACCATTGGAGGGGTATTATTTAGTTGTTGTGTTCAACAAAAAGATGGTGTTACTTTTAGGAGATCTGAAAAAGGAAGCATGCAAGAAGATTGATACTGACAACAATGTTAGTGGTAATAATTCTGATGCAATTTTTATTGCAAAGAGAGAACACATTTTTGGCAAGAAATGTTACTGTGCAAAGGCTCAATTTTGTGACAAGGGTAAGGTACATGATGTGAGAATTGAGTGTGATACACTTGTTGGGACTAATGAACCTTGTCTTGTGATTAGAATTGATAGCAAGATAGTGATGCAGGTGAAGCAATTGAAGTGGAAGTTTCGCGGTAATCAAACTGTTTTGGTGGATGGTTTTCCAGTTGAAGTGTTTTGGGATGTTCATAATTGGCTCTTTGGAAATGCTATGGGAAATGCTGTTTTTATGTTCCAAACTTGTATTTCAGCTGAGAAATTGTGGTCAGGTCAATCTGTTTCAGATCCTTCTCTTTTGACATGGGCTTATTCACATCAATTTAGAGATTCTCAGTTGCAAGGTCTTGGATTTTCTCTCATTTTGTATGCTTGGAAAAATGAGTAG |
| Protein sequence |
>MS.gene50493.t1 MKEFPSCFGENGVQVADSSSSSSSSIRGPQNVVTCIYQCNLRGRSCLITVSWTKNLMGQGLSVGIDELGNSCLCKVDIKPWLFSKRKGCKNLEVESSKVVILWDLSYAKFGSGPEPLEGYYLVVVFNKKMVLLLGDLKKEACKKIDTDNNVSGNNSDAIFIAKREHIFGKKCYCAKAQFCDKGKVHDVRIECDTLVGTNEPCLVIRIDSKIVMQVKQLKWKFRGNQTVLVDGFPVEVFWDVHNWLFGNAMGNAVFMFQTCISAEKLWSGQSVSDPSLLTWAYSHQFRDSQLQGLGFSLILYAWKNE |
