AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50493


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50493.t1 MTR_7g095100 98.046 307 5 1 1 306 1 307 0.0 613
MS.gene50493.t1 MTR_1g081260 64.610 308 102 3 1 306 1 303 9.51e-144 407
MS.gene50493.t1 MTR_7g114790 62.581 310 106 4 1 305 1 305 3.60e-139 395
MS.gene50493.t1 MTR_5g069570 40.351 285 153 7 30 306 34 309 1.61e-64 206
MS.gene50493.t1 MTR_3g076610 36.396 283 151 5 5 266 8 282 1.19e-60 196
MS.gene50493.t1 MTR_4g104760 38.722 266 144 5 5 258 8 266 2.30e-60 195
MS.gene50493.t1 MTR_4g055230 37.152 323 165 11 1 304 1 304 1.25e-57 187
MS.gene50493.t1 MTR_3g082430 39.241 237 134 5 27 258 13 244 7.75e-55 180
MS.gene50493.t1 MTR_7g103900 36.201 279 153 8 1 259 1 274 5.77e-52 173
MS.gene50493.t1 MTR_2g084965 38.686 274 142 8 6 259 8 275 4.87e-49 166
MS.gene50493.t1 MTR_3g106250 35.556 270 144 8 4 258 6 260 6.65e-49 166
MS.gene50493.t1 MTR_5g096570 33.333 279 176 5 29 304 1189 1460 2.87e-48 173
MS.gene50493.t1 MTR_1g013100 29.706 340 178 14 4 304 8 325 1.15e-37 136
MS.gene50493.t1 MTR_7g103900 32.479 234 136 7 1 217 1 229 3.79e-32 119
MS.gene50493.t1 MTR_8g069535 63.415 82 29 1 8 89 9 89 1.26e-24 99.8
MS.gene50493.t1 MTR_8g069535 50.769 65 31 1 175 239 84 147 2.79e-12 65.9
MS.gene50493.t1 MTR_5g069550 32.000 175 99 3 10 182 14 170 4.41e-22 91.7
MS.gene50493.t1 MTR_0716s0040 39.241 79 26 1 200 278 15 71 1.93e-13 64.7
MS.gene50493.t1 MTR_0716s0020 58.824 51 17 1 56 106 3 49 3.40e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50493.t1 AT5G28150 60.261 307 103 6 1 306 1 289 2.40e-120 347
MS.gene50493.t1 AT3G04860 57.143 308 111 5 1 306 1 289 7.90e-120 346
MS.gene50493.t1 AT2G04220 42.169 249 134 5 14 258 12 254 1.82e-63 203
MS.gene50493.t1 AT4G12690 41.129 248 135 5 15 258 9 249 3.88e-62 199
MS.gene50493.t1 AT4G12690 41.129 248 135 5 15 258 9 249 3.88e-62 199
MS.gene50493.t1 AT5G48270 37.805 328 160 12 5 306 13 322 4.29e-56 184
MS.gene50493.t1 AT2G27770 32.143 336 179 11 1 304 1 319 2.18e-48 164
MS.gene50493.t1 AT3G13229 34.188 234 138 8 33 255 8 236 2.21e-37 135
MS.gene50493.t1 AT5G11000 32.432 259 131 10 28 258 30 272 9.29e-35 130
MS.gene50493.t1 AT2G25200 25.138 362 184 12 6 304 16 353 1.99e-30 118
MS.gene50493.t1 AT2G36470 29.927 274 146 11 1 250 1 252 2.98e-24 100

Find 49 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTATGCTAAATTTGGTTC+TGG 0.191766 7.3:+16047888 MS.gene50493:CDS
GAGAAAAGGGTGTAAGAATT+TGG 0.248105 7.3:+16047827 MS.gene50493:CDS
GATTTGAGTTATGCTAAATT+TGG 0.254797 7.3:+16047882 MS.gene50493:CDS
GATGTTCATAATTGGCTCTT+TGG 0.260181 7.3:+16048290 MS.gene50493:CDS
CATGTTTGATTACAGTTTCA+TGG 0.295709 7.3:+16047706 MS.gene50493:CDS
AAGGAGTTTCCATCTTGTTT+TGG 0.305139 7.3:+16047576 MS.gene50493:CDS
TAGTAAAGTTGTTATACTTT+GGG 0.342797 7.3:+16047860 MS.gene50493:CDS
GATTCTCAGTTGCAAGGTCT+TGG 0.351714 7.3:+16048434 MS.gene50493:CDS
CTAGTAAAGTTGTTATACTT+TGG 0.351998 7.3:+16047859 MS.gene50493:CDS
TCGCGGTAATCAAACTGTTT+TGG 0.353773 7.3:+16048238 MS.gene50493:CDS
TCTCAGCTGAAATACAAGTT+TGG 0.362558 7.3:-16048343 None:intergenic
TCCTCTTCAAGTAGCATAAG+AGG 0.389142 7.3:+16047633 MS.gene50493:CDS
TAATACCCCTCCAATGGTTC+TGG 0.393388 7.3:-16047912 None:intergenic
GTTATGCTAAATTTGGTTCT+GGG 0.396684 7.3:+16047889 MS.gene50493:CDS
ACATCCCAAAACACTTCAAC+TGG 0.400830 7.3:-16048272 None:intergenic
TAGTGTTGGAATTGATGAAT+TGG 0.416664 7.3:+16047755 MS.gene50493:CDS
CAATTCCAACACTAAGGCCT+TGG 0.444766 7.3:-16047746 None:intergenic
ATCTATCAATGTAATTTAAG+AGG 0.448731 7.3:+16047678 MS.gene50493:CDS
TTGGCTCTTTGGAAATGCTA+TGG 0.452459 7.3:+16048301 MS.gene50493:CDS
TTCTGGGCCAGAACCATTGG+AGG 0.456488 7.3:+16047905 MS.gene50493:CDS
AGTGTTGGAATTGATGAATT+GGG 0.473318 7.3:+16047756 MS.gene50493:CDS
GATACTGACAACAATGTTAG+TGG 0.473769 7.3:+16048008 MS.gene50493:CDS
GGGAAATAGTTGTTTATGCA+AGG 0.478641 7.3:+16047776 MS.gene50493:CDS
TTCATGGACAAAGAATTTGA+TGG 0.485530 7.3:+16047722 MS.gene50493:CDS
TGGCTCTTTGGAAATGCTAT+GGG 0.488711 7.3:+16048302 MS.gene50493:CDS
ATTGAGTGTGATACACTTGT+TGG 0.493306 7.3:+16048140 MS.gene50493:CDS
TGGTTCTGGGCCAGAACCAT+TGG 0.500620 7.3:+16047902 MS.gene50493:CDS
ATGGGCCAAGGCCTTAGTGT+TGG 0.505702 7.3:+16047741 MS.gene50493:CDS
TCATGGACAAAGAATTTGAT+GGG 0.518931 7.3:+16047723 MS.gene50493:CDS
TCAGCTGAGAAATTGTGGTC+AGG 0.522661 7.3:+16048356 MS.gene50493:CDS
TGATAGCAAGATAGTGATGC+AGG 0.524536 7.3:+16048193 MS.gene50493:CDS
CAATTGAAGTGGAAGTTTCG+CGG 0.531065 7.3:+16048221 MS.gene50493:CDS
GTATTTCAGCTGAGAAATTG+TGG 0.534611 7.3:+16048351 MS.gene50493:CDS
CCCTCTTATGCTACTTGAAG+AGG 0.536585 7.3:-16047634 None:intergenic
ATTCATCAATTCCAACACTA+AGG 0.542567 7.3:-16047752 None:intergenic
TTTAGAGATTCTCAGTTGCA+AGG 0.549501 7.3:+16048428 MS.gene50493:CDS
CATAAGAGGGCCACAAAATG+TGG 0.551229 7.3:+16047647 MS.gene50493:CDS
TTGAGTGTGATACACTTGTT+GGG 0.554324 7.3:+16048141 MS.gene50493:CDS
TTTCTCTTTGAAAACAACCA+TGG 0.574868 7.3:-16047810 None:intergenic
ACAAAGAATTTGATGGGCCA+AGG 0.577061 7.3:+16047729 MS.gene50493:CDS
ACTAAATAATACCCCTCCAA+TGG 0.586748 7.3:-16047918 None:intergenic
TAAGCCCATGTCAAAAGAGA+AGG 0.587808 7.3:-16048395 None:intergenic
CAAGAAATGTTACTGTGCAA+AGG 0.597482 7.3:+16048079 MS.gene50493:CDS
TGCAGGTGAAGCAATTGAAG+TGG 0.628266 7.3:+16048210 MS.gene50493:CDS
CCTCTTCAAGTAGCATAAGA+GGG 0.631812 7.3:+16047634 MS.gene50493:CDS
GCAAGGTTGATATAAAGCCA+TGG 0.638293 7.3:+16047793 MS.gene50493:CDS
TCTGGGCCAGAACCATTGGA+GGG 0.642416 7.3:+16047906 MS.gene50493:CDS
TCAATTCTAATCACAAGACA+AGG 0.678608 7.3:-16048173 None:intergenic
CTGGGCCAGAACCATTGGAG+GGG 0.724778 7.3:+16047907 MS.gene50493:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTATCAATGTAATTTAAG+AGG + chr7.3:16047678-16047697 MS.gene50493:CDS 20.0%
!! TAGTAAAGTTGTTATACTTT+GGG + chr7.3:16047860-16047879 MS.gene50493:CDS 20.0%
! CTAGTAAAGTTGTTATACTT+TGG + chr7.3:16047859-16047878 MS.gene50493:CDS 25.0%
!! GATTTGAGTTATGCTAAATT+TGG + chr7.3:16047882-16047901 MS.gene50493:CDS 25.0%
!!! AAAAAGATGGTGTTACTTTT+AGG + chr7.3:16047954-16047973 MS.gene50493:CDS 25.0%
ATTCATCAATTCCAACACTA+AGG - chr7.3:16047755-16047774 None:intergenic 30.0%
CATGTTTGATTACAGTTTCA+TGG + chr7.3:16047706-16047725 MS.gene50493:CDS 30.0%
TCAATTCTAATCACAAGACA+AGG - chr7.3:16048176-16048195 None:intergenic 30.0%
TCATGGACAAAGAATTTGAT+GGG + chr7.3:16047723-16047742 MS.gene50493:CDS 30.0%
TGTTGTGTTCAACAAAAAGA+TGG + chr7.3:16047941-16047960 MS.gene50493:CDS 30.0%
TTCATGGACAAAGAATTTGA+TGG + chr7.3:16047722-16047741 MS.gene50493:CDS 30.0%
! AGTGTTGGAATTGATGAATT+GGG + chr7.3:16047756-16047775 MS.gene50493:CDS 30.0%
! AGTTATGCTAAATTTGGTTC+TGG + chr7.3:16047888-16047907 MS.gene50493:CDS 30.0%
! ATACAAGTAACCACATTTTG+TGG - chr7.3:16047660-16047679 None:intergenic 30.0%
! TAGTGTTGGAATTGATGAAT+TGG + chr7.3:16047755-16047774 MS.gene50493:CDS 30.0%
! TTTCTCTCATTTTGTATGCT+TGG + chr7.3:16048459-16048478 MS.gene50493:CDS 30.0%
! TTTCTCTTTGAAAACAACCA+TGG - chr7.3:16047813-16047832 None:intergenic 30.0%
!! ACTTTTAGGAGATCTGAAAA+AGG + chr7.3:16047968-16047987 MS.gene50493:CDS 30.0%
!! GGTTGTTTTCAAAGAGAAAA+GGG + chr7.3:16047814-16047833 MS.gene50493:CDS 30.0%
!! GTTATGCTAAATTTGGTTCT+GGG + chr7.3:16047889-16047908 MS.gene50493:CDS 30.0%
!! TGGTTGTTTTCAAAGAGAAA+AGG + chr7.3:16047813-16047832 MS.gene50493:CDS 30.0%
!! TTTTCCAGTTGAAGTGTTTT+GGG + chr7.3:16048268-16048287 MS.gene50493:CDS 30.0%
!!! TGTTTTGGGATGTTCATAAT+TGG + chr7.3:16048282-16048301 MS.gene50493:CDS 30.0%
ACTAAATAATACCCCTCCAA+TGG - chr7.3:16047921-16047940 None:intergenic 35.0%
ATTGAGTGTGATACACTTGT+TGG + chr7.3:16048140-16048159 MS.gene50493:CDS 35.0%
CAAGAAATGTTACTGTGCAA+AGG + chr7.3:16048079-16048098 MS.gene50493:CDS 35.0%
GATACTGACAACAATGTTAG+TGG + chr7.3:16048008-16048027 MS.gene50493:CDS 35.0%
GGGAAATAGTTGTTTATGCA+AGG + chr7.3:16047776-16047795 MS.gene50493:CDS 35.0%
GTATTTCAGCTGAGAAATTG+TGG + chr7.3:16048351-16048370 MS.gene50493:CDS 35.0%
TCTCAGCTGAAATACAAGTT+TGG - chr7.3:16048346-16048365 None:intergenic 35.0%
TTGAGTGTGATACACTTGTT+GGG + chr7.3:16048141-16048160 MS.gene50493:CDS 35.0%
TTTAGAGATTCTCAGTTGCA+AGG + chr7.3:16048428-16048447 MS.gene50493:CDS 35.0%
! AAAGGCTCAATTTTGTGACA+AGG + chr7.3:16048097-16048116 MS.gene50493:CDS 35.0%
! AAGGCTCAATTTTGTGACAA+GGG + chr7.3:16048098-16048117 MS.gene50493:CDS 35.0%
! AGATCCTTCTCTTTTGACAT+GGG + chr7.3:16048391-16048410 MS.gene50493:CDS 35.0%
! CCATTTTCTCCAAAACAAGA+TGG - chr7.3:16047588-16047607 None:intergenic 35.0%
! GAGAAAAGGGTGTAAGAATT+TGG + chr7.3:16047827-16047846 MS.gene50493:CDS 35.0%
! GATGTTCATAATTGGCTCTT+TGG + chr7.3:16048290-16048309 MS.gene50493:CDS 35.0%
! GCAAAGAGAGAACACATTTT+TGG + chr7.3:16048056-16048075 MS.gene50493:CDS 35.0%
! TCAATTTTGTGACAAGGGTA+AGG + chr7.3:16048103-16048122 MS.gene50493:CDS 35.0%
!! AAGGAGTTTCCATCTTGTTT+TGG + chr7.3:16047576-16047595 MS.gene50493:CDS 35.0%
!! GTTTTCCAGTTGAAGTGTTT+TGG + chr7.3:16048267-16048286 MS.gene50493:CDS 35.0%
!! TTTTGGAGAAAATGGTGTTC+AGG + chr7.3:16047593-16047612 MS.gene50493:CDS 35.0%
!!! AATCAAACTGTTTTGGTGGA+TGG + chr7.3:16048245-16048264 MS.gene50493:CDS 35.0%
!!! CCATCTTGTTTTGGAGAAAA+TGG + chr7.3:16047585-16047604 MS.gene50493:CDS 35.0%
ACATCCCAAAACACTTCAAC+TGG - chr7.3:16048275-16048294 None:intergenic 40.0%
CAATTGAAGTGGAAGTTTCG+CGG + chr7.3:16048221-16048240 MS.gene50493:CDS 40.0%
CCTCTTCAAGTAGCATAAGA+GGG + chr7.3:16047634-16047653 MS.gene50493:CDS 40.0%
TAAGCCCATGTCAAAAGAGA+AGG - chr7.3:16048398-16048417 None:intergenic 40.0%
TCCTCTTCAAGTAGCATAAG+AGG + chr7.3:16047633-16047652 MS.gene50493:CDS 40.0%
TGATAGCAAGATAGTGATGC+AGG + chr7.3:16048193-16048212 MS.gene50493:CDS 40.0%
! ACAAAGAATTTGATGGGCCA+AGG + chr7.3:16047729-16047748 MS.gene50493:CDS 40.0%
! CAGATCCTTCTCTTTTGACA+TGG + chr7.3:16048390-16048409 MS.gene50493:CDS 40.0%
! TCGCGGTAATCAAACTGTTT+TGG + chr7.3:16048238-16048257 MS.gene50493:CDS 40.0%
! TGGCTCTTTGGAAATGCTAT+GGG + chr7.3:16048302-16048321 MS.gene50493:CDS 40.0%
! TTGGCTCTTTGGAAATGCTA+TGG + chr7.3:16048301-16048320 MS.gene50493:CDS 40.0%
!! CGGTAATCAAACTGTTTTGG+TGG + chr7.3:16048241-16048260 MS.gene50493:CDS 40.0%
!! GCAAGGTTGATATAAAGCCA+TGG + chr7.3:16047793-16047812 MS.gene50493:CDS 40.0%
CAATTCCAACACTAAGGCCT+TGG - chr7.3:16047749-16047768 None:intergenic 45.0%
CATAAGAGGGCCACAAAATG+TGG + chr7.3:16047647-16047666 MS.gene50493:CDS 45.0%
GATTCTCAGTTGCAAGGTCT+TGG + chr7.3:16048434-16048453 MS.gene50493:CDS 45.0%
TAATACCCCTCCAATGGTTC+TGG - chr7.3:16047915-16047934 None:intergenic 45.0%
TCAGCTGAGAAATTGTGGTC+AGG + chr7.3:16048356-16048375 MS.gene50493:CDS 45.0%
TGCAGGTGAAGCAATTGAAG+TGG + chr7.3:16048210-16048229 MS.gene50493:CDS 45.0%
! CCCTCTTATGCTACTTGAAG+AGG - chr7.3:16047637-16047656 None:intergenic 45.0%
ATGGGCCAAGGCCTTAGTGT+TGG + chr7.3:16047741-16047760 MS.gene50493:CDS 55.0%
TCTGGGCCAGAACCATTGGA+GGG + chr7.3:16047906-16047925 MS.gene50493:CDS 55.0%
TTCTGGGCCAGAACCATTGG+AGG + chr7.3:16047905-16047924 MS.gene50493:CDS 55.0%
!! TGGTTCTGGGCCAGAACCAT+TGG + chr7.3:16047902-16047921 MS.gene50493:CDS 55.0%
CTGGGCCAGAACCATTGGAG+GGG + chr7.3:16047907-16047926 MS.gene50493:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 16047573 16048493 16047573 ID=MS.gene50493
chr7.3 mRNA 16047573 16048493 16047573 ID=MS.gene50493.t1;Parent=MS.gene50493
chr7.3 exon 16047573 16048493 16047573 ID=MS.gene50493.t1.exon1;Parent=MS.gene50493.t1
chr7.3 CDS 16047573 16048493 16047573 ID=cds.MS.gene50493.t1;Parent=MS.gene50493.t1
Gene Sequence

>MS.gene50493

ATGAAGGAGTTTCCATCTTGTTTTGGAGAAAATGGTGTTCAGGTTGCAGATTCATCTTCATCCTCTTCAAGTAGCATAAGAGGGCCACAAAATGTGGTTACTTGTATCTATCAATGTAATTTAAGAGGTCGTTCATGTTTGATTACAGTTTCATGGACAAAGAATTTGATGGGCCAAGGCCTTAGTGTTGGAATTGATGAATTGGGAAATAGTTGTTTATGCAAGGTTGATATAAAGCCATGGTTGTTTTCAAAGAGAAAAGGGTGTAAGAATTTGGAAGTTGAATCTAGTAAAGTTGTTATACTTTGGGATTTGAGTTATGCTAAATTTGGTTCTGGGCCAGAACCATTGGAGGGGTATTATTTAGTTGTTGTGTTCAACAAAAAGATGGTGTTACTTTTAGGAGATCTGAAAAAGGAAGCATGCAAGAAGATTGATACTGACAACAATGTTAGTGGTAATAATTCTGATGCAATTTTTATTGCAAAGAGAGAACACATTTTTGGCAAGAAATGTTACTGTGCAAAGGCTCAATTTTGTGACAAGGGTAAGGTACATGATGTGAGAATTGAGTGTGATACACTTGTTGGGACTAATGAACCTTGTCTTGTGATTAGAATTGATAGCAAGATAGTGATGCAGGTGAAGCAATTGAAGTGGAAGTTTCGCGGTAATCAAACTGTTTTGGTGGATGGTTTTCCAGTTGAAGTGTTTTGGGATGTTCATAATTGGCTCTTTGGAAATGCTATGGGAAATGCTGTTTTTATGTTCCAAACTTGTATTTCAGCTGAGAAATTGTGGTCAGGTCAATCTGTTTCAGATCCTTCTCTTTTGACATGGGCTTATTCACATCAATTTAGAGATTCTCAGTTGCAAGGTCTTGGATTTTCTCTCATTTTGTATGCTTGGAAAAATGAGTAG

Protein sequence

>MS.gene50493.t1

MKEFPSCFGENGVQVADSSSSSSSSIRGPQNVVTCIYQCNLRGRSCLITVSWTKNLMGQGLSVGIDELGNSCLCKVDIKPWLFSKRKGCKNLEVESSKVVILWDLSYAKFGSGPEPLEGYYLVVVFNKKMVLLLGDLKKEACKKIDTDNNVSGNNSDAIFIAKREHIFGKKCYCAKAQFCDKGKVHDVRIECDTLVGTNEPCLVIRIDSKIVMQVKQLKWKFRGNQTVLVDGFPVEVFWDVHNWLFGNAMGNAVFMFQTCISAEKLWSGQSVSDPSLLTWAYSHQFRDSQLQGLGFSLILYAWKNE