Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56278.t1 | XP_003598279.1 | 92 | 500 | 32 | 1 | 1 | 492 | 27 | 526 | 4.10E-269 | 936.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56278.t1 | Q9SUC6 | 55.4 | 502 | 204 | 8 | 3 | 488 | 32 | 529 | 3.7e-162 | 572.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56278.t1 | G7J0C5 | 92.0 | 500 | 32 | 1 | 1 | 492 | 27 | 526 | 3.0e-269 | 936.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059511 | MS.gene56278 | 0.80925 | 2.08E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene02146 | MS.gene56278 | PPI |
| MS.gene56278 | MS.gene36444 | PPI |
| MS.gene028357 | MS.gene56278 | PPI |
| MS.gene56278 | MS.gene028354 | PPI |
| MS.gene35114 | MS.gene56278 | PPI |
| MS.gene35113 | MS.gene56278 | PPI |
| MS.gene56278 | MS.gene36437 | PPI |
| MS.gene36421 | MS.gene56278 | PPI |
| MS.gene28726 | MS.gene56278 | PPI |
| MS.gene35083 | MS.gene56278 | PPI |
| MS.gene28717 | MS.gene56278 | PPI |
| MS.gene56278 | MS.gene36435 | PPI |
| MS.gene28723 | MS.gene56278 | PPI |
| MS.gene028352 | MS.gene56278 | PPI |
| MS.gene35102 | MS.gene56278 | PPI |
| MS.gene56278 | MS.gene35103 | PPI |
| MS.gene28742 | MS.gene56278 | PPI |
| MS.gene36445 | MS.gene56278 | PPI |
| MS.gene28725 | MS.gene56278 | PPI |
| MS.gene36439 | MS.gene56278 | PPI |
| MS.gene56278 | MS.gene028346 | PPI |
| MS.gene028369 | MS.gene56278 | PPI |
| MS.gene35106 | MS.gene56278 | PPI |
| MS.gene56278 | MS.gene047977 | PPI |
| MS.gene28727 | MS.gene56278 | PPI |
| MS.gene35104 | MS.gene56278 | PPI |
| MS.gene35105 | MS.gene56278 | PPI |
| MS.gene36438 | MS.gene56278 | PPI |
| MS.gene003357 | MS.gene56278 | PPI |
| MS.gene28724 | MS.gene56278 | PPI |
| MS.gene56278 | MS.gene028353 | PPI |
| MS.gene028347 | MS.gene56278 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56278.t1 | MTR_3g009820 | 92.000 | 500 | 32 | 1 | 1 | 492 | 27 | 526 | 0.0 | 934 |
| MS.gene56278.t1 | MTR_3g009760 | 91.600 | 500 | 34 | 1 | 1 | 492 | 27 | 526 | 0.0 | 932 |
| MS.gene56278.t1 | MTR_3g009880 | 86.172 | 499 | 59 | 2 | 1 | 491 | 27 | 523 | 0.0 | 868 |
| MS.gene56278.t1 | MTR_2g031420 | 57.230 | 491 | 192 | 7 | 12 | 489 | 43 | 528 | 0.0 | 545 |
| MS.gene56278.t1 | MTR_2g031390 | 54.545 | 473 | 198 | 6 | 31 | 488 | 59 | 529 | 0.0 | 545 |
| MS.gene56278.t1 | MTR_4g094488 | 55.050 | 505 | 205 | 7 | 1 | 486 | 34 | 535 | 0.0 | 545 |
| MS.gene56278.t1 | MTR_2g031460 | 55.400 | 500 | 199 | 9 | 1 | 485 | 34 | 524 | 0.0 | 539 |
| MS.gene56278.t1 | MTR_4g094492 | 54.420 | 509 | 209 | 8 | 1 | 490 | 34 | 538 | 0.0 | 530 |
| MS.gene56278.t1 | MTR_4g094478 | 52.976 | 504 | 219 | 6 | 1 | 488 | 34 | 535 | 0.0 | 529 |
| MS.gene56278.t1 | MTR_4g094495 | 54.167 | 504 | 211 | 6 | 1 | 486 | 37 | 538 | 0.0 | 528 |
| MS.gene56278.t1 | MTR_4g094518 | 54.365 | 504 | 209 | 9 | 1 | 488 | 32 | 530 | 0.0 | 526 |
| MS.gene56278.t1 | MTR_4g094418 | 56.881 | 436 | 170 | 5 | 72 | 489 | 1 | 436 | 1.89e-180 | 520 |
| MS.gene56278.t1 | MTR_4g094520 | 52.183 | 481 | 214 | 6 | 22 | 488 | 54 | 532 | 4.09e-179 | 513 |
| MS.gene56278.t1 | MTR_4g094480 | 51.394 | 502 | 226 | 7 | 1 | 486 | 34 | 533 | 5.71e-179 | 513 |
| MS.gene56278.t1 | MTR_4g094498 | 51.303 | 499 | 230 | 5 | 2 | 490 | 41 | 536 | 1.02e-178 | 513 |
| MS.gene56278.t1 | MTR_2g031560 | 48.902 | 501 | 240 | 6 | 1 | 488 | 26 | 523 | 4.04e-178 | 511 |
| MS.gene56278.t1 | MTR_2g031590 | 50.204 | 490 | 218 | 8 | 23 | 488 | 45 | 532 | 2.99e-172 | 496 |
| MS.gene56278.t1 | MTR_2g031510 | 46.748 | 492 | 242 | 7 | 14 | 488 | 52 | 540 | 3.80e-158 | 460 |
| MS.gene56278.t1 | MTR_2g031490 | 46.107 | 488 | 245 | 6 | 17 | 488 | 53 | 538 | 7.07e-156 | 454 |
| MS.gene56278.t1 | MTR_4g088355 | 46.813 | 502 | 246 | 7 | 9 | 490 | 35 | 535 | 1.23e-154 | 451 |
| MS.gene56278.t1 | MTR_4g091200 | 47.531 | 486 | 238 | 7 | 15 | 486 | 42 | 524 | 1.58e-154 | 450 |
| MS.gene56278.t1 | MTR_4g057990 | 45.766 | 496 | 258 | 4 | 1 | 486 | 26 | 520 | 4.30e-154 | 449 |
| MS.gene56278.t1 | MTR_4g091150 | 49.565 | 460 | 216 | 6 | 44 | 488 | 82 | 540 | 5.28e-153 | 447 |
| MS.gene56278.t1 | MTR_4g091190 | 47.119 | 486 | 233 | 8 | 22 | 486 | 44 | 526 | 9.93e-153 | 446 |
| MS.gene56278.t1 | MTR_3g009850 | 87.931 | 232 | 28 | 0 | 261 | 492 | 1 | 232 | 4.23e-151 | 430 |
| MS.gene56278.t1 | MTR_6g017205 | 46.029 | 491 | 248 | 7 | 10 | 486 | 37 | 524 | 7.08e-150 | 439 |
| MS.gene56278.t1 | MTR_4g094515 | 47.799 | 477 | 233 | 6 | 28 | 490 | 678 | 1152 | 2.08e-148 | 453 |
| MS.gene56278.t1 | MTR_4g094515 | 46.139 | 505 | 246 | 10 | 1 | 486 | 41 | 538 | 4.66e-146 | 447 |
| MS.gene56278.t1 | MTR_4g091170 | 46.460 | 452 | 212 | 6 | 47 | 486 | 107 | 540 | 1.33e-146 | 432 |
| MS.gene56278.t1 | MTR_2g023590 | 46.087 | 460 | 232 | 6 | 44 | 490 | 12 | 468 | 3.31e-140 | 412 |
| MS.gene56278.t1 | MTR_1g076830 | 41.895 | 475 | 257 | 8 | 28 | 485 | 60 | 532 | 3.58e-120 | 363 |
| MS.gene56278.t1 | MTR_2g031600 | 50.251 | 199 | 84 | 3 | 23 | 206 | 46 | 244 | 2.25e-58 | 198 |
| MS.gene56278.t1 | MTR_2g031600 | 53.797 | 158 | 70 | 2 | 332 | 488 | 245 | 400 | 2.81e-53 | 185 |
| MS.gene56278.t1 | MTR_2g084370 | 73.786 | 103 | 13 | 1 | 96 | 198 | 1 | 89 | 1.20e-44 | 152 |
| MS.gene56278.t1 | MTR_2g084405 | 74.118 | 85 | 12 | 2 | 96 | 170 | 1 | 85 | 3.79e-28 | 108 |
| MS.gene56278.t1 | MTR_8g062280 | 47.458 | 59 | 29 | 1 | 434 | 492 | 21 | 77 | 3.20e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56278.t1 | AT4G20860 | 59.121 | 455 | 174 | 5 | 44 | 488 | 77 | 529 | 0.0 | 553 |
| MS.gene56278.t1 | AT5G44360 | 53.529 | 510 | 202 | 12 | 1 | 488 | 50 | 546 | 0.0 | 531 |
| MS.gene56278.t1 | AT5G44360 | 53.529 | 510 | 202 | 12 | 1 | 488 | 34 | 530 | 0.0 | 531 |
| MS.gene56278.t1 | AT5G44400 | 54.825 | 487 | 202 | 10 | 16 | 486 | 50 | 534 | 0.0 | 525 |
| MS.gene56278.t1 | AT1G30760 | 52.556 | 489 | 209 | 8 | 13 | 486 | 68 | 548 | 5.11e-178 | 511 |
| MS.gene56278.t1 | AT1G30760 | 52.556 | 489 | 209 | 8 | 13 | 486 | 50 | 530 | 6.66e-178 | 510 |
| MS.gene56278.t1 | AT2G34790 | 51.667 | 480 | 211 | 7 | 22 | 486 | 55 | 528 | 2.80e-177 | 508 |
| MS.gene56278.t1 | AT5G44390 | 53.958 | 480 | 200 | 11 | 27 | 488 | 64 | 540 | 5.10e-175 | 503 |
| MS.gene56278.t1 | AT5G44440 | 47.358 | 492 | 239 | 9 | 16 | 491 | 45 | 532 | 2.42e-161 | 468 |
| MS.gene56278.t1 | AT5G44440 | 48.218 | 477 | 227 | 9 | 31 | 491 | 130 | 602 | 2.31e-160 | 468 |
| MS.gene56278.t1 | AT5G44380 | 51.129 | 487 | 220 | 10 | 16 | 486 | 53 | 537 | 5.99e-160 | 464 |
| MS.gene56278.t1 | AT5G44380 | 51.129 | 487 | 220 | 10 | 16 | 486 | 93 | 577 | 8.90e-160 | 466 |
| MS.gene56278.t1 | AT4G20820 | 48.085 | 470 | 225 | 8 | 28 | 486 | 63 | 524 | 5.20e-159 | 462 |
| MS.gene56278.t1 | AT1G26420 | 48.276 | 493 | 234 | 7 | 11 | 486 | 35 | 523 | 2.90e-158 | 460 |
| MS.gene56278.t1 | AT1G26410 | 48.745 | 478 | 226 | 7 | 22 | 486 | 76 | 547 | 2.05e-155 | 453 |
| MS.gene56278.t1 | AT1G26380 | 48.347 | 484 | 235 | 5 | 17 | 488 | 45 | 525 | 1.03e-154 | 451 |
| MS.gene56278.t1 | AT1G26390 | 48.643 | 479 | 227 | 7 | 22 | 486 | 52 | 525 | 1.42e-154 | 451 |
| MS.gene56278.t1 | AT5G44410 | 47.908 | 478 | 223 | 10 | 28 | 488 | 61 | 529 | 7.84e-151 | 441 |
| MS.gene56278.t1 | AT1G26400 | 46.626 | 489 | 245 | 7 | 9 | 485 | 37 | 521 | 6.39e-150 | 439 |
| MS.gene56278.t1 | AT1G30700 | 47.379 | 477 | 231 | 8 | 28 | 488 | 54 | 526 | 8.47e-148 | 433 |
| MS.gene56278.t1 | AT1G34575 | 43.478 | 506 | 250 | 11 | 2 | 487 | 34 | 523 | 4.81e-147 | 431 |
| MS.gene56278.t1 | AT1G30710 | 43.762 | 505 | 260 | 9 | 2 | 490 | 34 | 530 | 1.17e-146 | 430 |
| MS.gene56278.t1 | AT1G11770 | 42.460 | 504 | 275 | 7 | 1 | 492 | 33 | 533 | 8.84e-144 | 423 |
| MS.gene56278.t1 | AT4G20800 | 46.610 | 472 | 234 | 7 | 34 | 491 | 61 | 528 | 3.16e-143 | 421 |
| MS.gene56278.t1 | AT1G01980 | 43.836 | 511 | 263 | 10 | 1 | 492 | 33 | 538 | 1.34e-142 | 420 |
| MS.gene56278.t1 | AT4G20840 | 42.829 | 502 | 268 | 8 | 1 | 488 | 39 | 535 | 1.87e-141 | 417 |
| MS.gene56278.t1 | AT1G30730 | 44.400 | 500 | 261 | 7 | 1 | 487 | 29 | 524 | 4.41e-141 | 416 |
| MS.gene56278.t1 | AT4G20830 | 44.748 | 476 | 247 | 7 | 28 | 490 | 66 | 538 | 1.10e-137 | 408 |
| MS.gene56278.t1 | AT4G20830 | 44.726 | 474 | 246 | 7 | 28 | 488 | 66 | 536 | 1.11e-136 | 406 |
| MS.gene56278.t1 | AT1G30740 | 43.227 | 502 | 268 | 8 | 1 | 488 | 30 | 528 | 2.51e-135 | 401 |
| MS.gene56278.t1 | AT1G30740 | 43.227 | 502 | 268 | 8 | 1 | 488 | 33 | 531 | 2.61e-135 | 402 |
| MS.gene56278.t1 | AT2G34810 | 42.376 | 505 | 273 | 7 | 1 | 488 | 36 | 539 | 7.95e-134 | 398 |
| MS.gene56278.t1 | AT1G30720 | 42.918 | 473 | 254 | 6 | 28 | 487 | 56 | 525 | 1.62e-133 | 397 |
Find 90 sgRNAs with CRISPR-Local
Find 125 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGGCATCAAGTACTTTA+AGG | 0.182488 | 3.1:+5055615 | MS.gene56278:CDS |
| AAGCTGGTTTAGAAGGTATT+TGG | 0.194170 | 3.1:+5055281 | MS.gene56278:CDS |
| GCTCACCTTATAGATGTAAA+TGG | 0.201621 | 3.1:+5054752 | MS.gene56278:CDS |
| AATGAGAGTTTCCCTGAATT+AGG | 0.214946 | 3.1:+5055097 | MS.gene56278:CDS |
| CACATTAGCATTACTTATAA+TGG | 0.248787 | 3.1:+5055324 | MS.gene56278:CDS |
| TTGTCTTGCAACCCTAATTC+AGG | 0.270690 | 3.1:-5055109 | None:intergenic |
| CATCCACTTTATATGCTTGT+TGG | 0.273212 | 3.1:-5055467 | None:intergenic |
| CGATGAATCTGCTTGGATTC+AGG | 0.282488 | 3.1:+5053899 | MS.gene56278:CDS |
| GGAGGTAGTGCTACTAGTTT+TGG | 0.283804 | 3.1:+5054827 | MS.gene56278:CDS |
| AAGTGGATGAGAATGCTTTA+TGG | 0.287403 | 3.1:+5055481 | MS.gene56278:CDS |
| GAGCTGCATATTACAACTAT+AGG | 0.298398 | 3.1:+5055536 | MS.gene56278:CDS |
| CTATTCAGCAAGAGTTCTAT+AGG | 0.301114 | 3.1:-5055196 | None:intergenic |
| CTTGATGAATTCAGCCATCT+TGG | 0.311842 | 3.1:-5053684 | None:intergenic |
| CACATCAGTGGAGGTGGATT+TGG | 0.339259 | 3.1:+5054683 | MS.gene56278:CDS |
| CAATACTTGTGGATACAATA+TGG | 0.349912 | 3.1:-5053643 | None:intergenic |
| AGAGAGAGTTAAACTTGGTT+TGG | 0.351945 | 3.1:-5055039 | None:intergenic |
| ATGAGAGTTTCCCTGAATTA+GGG | 0.357011 | 3.1:+5055098 | MS.gene56278:CDS |
| TCTAAACCAGCTTCTGGTAT+AGG | 0.365053 | 3.1:-5055271 | None:intergenic |
| GGTACTCAGAAGCAAGTGTT+TGG | 0.380263 | 3.1:+5055596 | MS.gene56278:CDS |
| ATTCAGGCTGGTGCAACATT+AGG | 0.380469 | 3.1:+5053915 | MS.gene56278:CDS |
| TCTAAAGCAAGTAAAGTTCA+TGG | 0.382983 | 3.1:+5053957 | MS.gene56278:CDS |
| TTACTTATAATGGAACCTTA+TGG | 0.388867 | 3.1:+5055334 | MS.gene56278:CDS |
| TGGGGCATCAAGTACTTTAA+GGG | 0.402731 | 3.1:+5055616 | MS.gene56278:CDS |
| ATACCTTCTAAACCAGCTTC+TGG | 0.406374 | 3.1:-5055277 | None:intergenic |
| ATACCAGAAGCTGGTTTAGA+AGG | 0.411336 | 3.1:+5055274 | MS.gene56278:CDS |
| CTTGGAGACTTTGAAACATA+AGG | 0.412215 | 3.1:-5055514 | None:intergenic |
| CTTGTACAACATACAATATA+TGG | 0.415255 | 3.1:+5055414 | MS.gene56278:CDS |
| AGCTGCATATTACAACTATA+GGG | 0.426482 | 3.1:+5055537 | MS.gene56278:CDS |
| CATATTGTATCCACAAGTAT+TGG | 0.430537 | 3.1:+5053644 | MS.gene56278:CDS |
| GTACTCAGAAGCAAGTGTTT+GGG | 0.433402 | 3.1:+5055597 | MS.gene56278:CDS |
| GATGAGATCAATCATGATAA+AGG | 0.434881 | 3.1:-5053847 | None:intergenic |
| TCATTCAGTCTTCCACCATA+AGG | 0.436662 | 3.1:-5055349 | None:intergenic |
| AAGTTTAACTCTCTCTTCCT+TGG | 0.446047 | 3.1:+5055046 | MS.gene56278:CDS |
| CTATGTATTGCCACCTATGA+AGG | 0.446403 | 3.1:-5054946 | None:intergenic |
| AGAATAGTTGCTCAAAATAG+TGG | 0.457619 | 3.1:+5054998 | MS.gene56278:CDS |
| TCATTCTTGCATGGAAGATA+AGG | 0.459271 | 3.1:+5054852 | MS.gene56278:CDS |
| CTCACCTTATAGATGTAAAT+GGG | 0.461407 | 3.1:+5054753 | MS.gene56278:CDS |
| AATTCTTGATAGAAAATCAA+TGG | 0.470027 | 3.1:+5054778 | MS.gene56278:CDS |
| TAAGGGAAACTTTAAGCGAT+TGG | 0.470910 | 3.1:+5055633 | MS.gene56278:CDS |
| AACTATAGGGATCTTGATAT+AGG | 0.475447 | 3.1:+5055550 | MS.gene56278:CDS |
| ATGAGATCAATCATGATAAA+GGG | 0.478729 | 3.1:-5053846 | None:intergenic |
| TAAGTTGTTAATTCAGGGGA+GGG | 0.480193 | 3.1:-5055725 | None:intergenic |
| TATTCAGCAAGAGTTCTATA+GGG | 0.484064 | 3.1:-5055195 | None:intergenic |
| ATCTTGCCGATGAATCTGCT+TGG | 0.489037 | 3.1:+5053892 | MS.gene56278:CDS |
| GAGCAACCAAGGTCCTTCAT+AGG | 0.491433 | 3.1:+5054933 | MS.gene56278:CDS |
| TTCAGTGATTCCAATACTTG+TGG | 0.491607 | 3.1:-5053654 | None:intergenic |
| TTCAGAAAAGCTACTACCTA+TGG | 0.493468 | 3.1:-5053586 | None:intergenic |
| AAGACAGTCCTTCATAATCA+TGG | 0.503011 | 3.1:-5053809 | None:intergenic |
| TAGTTGTAATATGCAGCTCT+TGG | 0.503584 | 3.1:-5055532 | None:intergenic |
| TGTCTTGCAACCCTAATTCA+GGG | 0.507182 | 3.1:-5055108 | None:intergenic |
| AATCCAACAAGCATATAAAG+TGG | 0.509234 | 3.1:+5055464 | MS.gene56278:CDS |
| ATGACTGCATTGAAATGAGT+TGG | 0.516911 | 3.1:+5055131 | MS.gene56278:CDS |
| ATTCTTGATAGAAAATCAAT+GGG | 0.522100 | 3.1:+5054779 | MS.gene56278:CDS |
| GTGGATTTGGTACCTTGGTA+AGG | 0.525170 | 3.1:+5054696 | MS.gene56278:CDS |
| CAAGATTAATGTCGATCAAA+CGG | 0.528579 | 3.1:-5053875 | None:intergenic |
| AAAGTTCATGGATTCCCTGC+AGG | 0.538278 | 3.1:+5053969 | MS.gene56278:CDS |
| GGTGGTCACATCAGTGGAGG+TGG | 0.539912 | 3.1:+5054677 | MS.gene56278:intron |
| ATTCAACATTCAAAGAACAT+TGG | 0.541115 | 3.1:+5054904 | MS.gene56278:CDS |
| TCTTGCATGGAAGATAAGGT+TGG | 0.542256 | 3.1:+5054856 | MS.gene56278:CDS |
| ATGCAGTGCATGTTAACCAT+AGG | 0.544066 | 3.1:+5053570 | MS.gene56278:CDS |
| TGAATTTCTGATTCATGAGA+AGG | 0.547415 | 3.1:-5053729 | None:intergenic |
| CACTGAAACAAAATCCAAGA+TGG | 0.547598 | 3.1:+5053670 | MS.gene56278:CDS |
| TGGAGGTGGATTTGGTACCT+TGG | 0.555699 | 3.1:+5054691 | MS.gene56278:CDS |
| TTGAATACTGTAACAATAGG+TGG | 0.557250 | 3.1:-5054887 | None:intergenic |
| AAAGAGCCTATACCAGAAGC+TGG | 0.561825 | 3.1:+5055265 | MS.gene56278:CDS |
| ATGTTGAATACTGTAACAAT+AGG | 0.562734 | 3.1:-5054890 | None:intergenic |
| GTGCAGATTAGAGTGGTTAG+TGG | 0.572020 | 3.1:+5053783 | MS.gene56278:CDS |
| CTGAATCCAAGCAGATTCAT+CGG | 0.574804 | 3.1:-5053898 | None:intergenic |
| ACATACAATATATGGTCAAG+TGG | 0.577424 | 3.1:+5055422 | MS.gene56278:CDS |
| GAATCTGCTTGGATTCAGGC+TGG | 0.577913 | 3.1:+5053903 | MS.gene56278:CDS |
| TTAAGTTGTTAATTCAGGGG+AGG | 0.578715 | 3.1:-5055726 | None:intergenic |
| AAGGAAGAGAGAGTTAAACT+TGG | 0.579262 | 3.1:-5055044 | None:intergenic |
| GGTACCTTGGTAAGGAAACA+TGG | 0.586233 | 3.1:+5054704 | MS.gene56278:CDS |
| AGTGGTGGCCATGATTATGA+AGG | 0.592283 | 3.1:+5053801 | MS.gene56278:CDS |
| CTTATAATGGAACCTTATGG+TGG | 0.592307 | 3.1:+5055337 | MS.gene56278:CDS |
| GATTGGTGTGCAGATTAGAG+TGG | 0.599674 | 3.1:+5053776 | MS.gene56278:CDS |
| GCAACAAGTATGACAATACA+AGG | 0.601545 | 3.1:+5055575 | MS.gene56278:CDS |
| TACTCAGAAGCAAGTGTTTG+GGG | 0.603811 | 3.1:+5055598 | MS.gene56278:CDS |
| CATACAATATATGGTCAAGT+GGG | 0.609377 | 3.1:+5055423 | MS.gene56278:CDS |
| GGAATAGGTGGTCACATCAG+TGG | 0.617717 | 3.1:+5054671 | MS.gene56278:intron |
| TTGCCACCTATGAAGGACCT+TGG | 0.622093 | 3.1:-5054939 | None:intergenic |
| CAACCAAGGTCCTTCATAGG+TGG | 0.629693 | 3.1:+5054936 | MS.gene56278:CDS |
| GCTCAAACTAATTACCTGCA+GGG | 0.638432 | 3.1:-5053983 | None:intergenic |
| ATTGGAACAAGGAGCAACCA+AGG | 0.639041 | 3.1:+5054922 | MS.gene56278:CDS |
| GAAGGTGTCATAATGAGAAG+AGG | 0.639842 | 3.1:-5053711 | None:intergenic |
| CAGGGGAGGGAATTCAGAAG+AGG | 0.643159 | 3.1:-5055712 | None:intergenic |
| TAGACCATGTTTCCTTACCA+AGG | 0.652475 | 3.1:-5054708 | None:intergenic |
| ATAGGTGGTCACATCAGTGG+AGG | 0.676455 | 3.1:+5054674 | MS.gene56278:intron |
| ATTCAAAGAACATTGGAACA+AGG | 0.678567 | 3.1:+5054911 | MS.gene56278:CDS |
| CAGATTAGAGTGGTTAGTGG+TGG | 0.684127 | 3.1:+5053786 | MS.gene56278:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAACTATAACAAA+CGG | + | chr3.1:5054464-5054483 | MS.gene56278:intron | 10.0% |
| !!! | AACTAATAATTTAGTTTTTT+CGG | + | chr3.1:5054287-5054306 | MS.gene56278:intron | 10.0% |
| !!! | AATTATAGTTATTTTTACAA+TGG | - | chr3.1:5054581-5054600 | None:intergenic | 10.0% |
| !!! | TTTTCTTAAAAAATAACTAA+CGG | + | chr3.1:5054541-5054560 | MS.gene56278:intron | 10.0% |
| !!! | TTATTTTAATATGTAGCAAA+CGG | - | chr3.1:5054176-5054195 | None:intergenic | 15.0% |
| !! | AATTCTTGATAGAAAATCAA+TGG | + | chr3.1:5054778-5054797 | MS.gene56278:CDS | 20.0% |
| !! | ATTCTTGATAGAAAATCAAT+GGG | + | chr3.1:5054779-5054798 | MS.gene56278:CDS | 20.0% |
| !!! | AAAAATTGATTTTTTGTTCG+AGG | - | chr3.1:5054383-5054402 | None:intergenic | 20.0% |
| !!! | ATCTAGTTTTGACTTTATTT+TGG | + | chr3.1:5054149-5054168 | MS.gene56278:intron | 20.0% |
| !!! | TTATAATTTGAAGCTGTTTT+TGG | + | chr3.1:5054423-5054442 | MS.gene56278:intron | 20.0% |
| ! | ATGAGATCAATCATGATAAA+GGG | - | chr3.1:5053849-5053868 | None:intergenic | 25.0% |
| ! | ATGTTGAATACTGTAACAAT+AGG | - | chr3.1:5054893-5054912 | None:intergenic | 25.0% |
| ! | ATTAAATTGCTTATCCAAAC+AGG | + | chr3.1:5054632-5054651 | MS.gene56278:intron | 25.0% |
| ! | ATTCAACATTCAAAGAACAT+TGG | + | chr3.1:5054904-5054923 | MS.gene56278:CDS | 25.0% |
| ! | CACATTAGCATTACTTATAA+TGG | + | chr3.1:5055324-5055343 | MS.gene56278:CDS | 25.0% |
| ! | CTTGTACAACATACAATATA+TGG | + | chr3.1:5055414-5055433 | MS.gene56278:CDS | 25.0% |
| !! | TTAAGTTATCTTTTTCTCCA+AGG | - | chr3.1:5055066-5055085 | None:intergenic | 25.0% |
| !! | TTACTTATAATGGAACCTTA+TGG | + | chr3.1:5055334-5055353 | MS.gene56278:CDS | 25.0% |
| !! | TTTTCCCATTTACATCTATA+AGG | - | chr3.1:5054760-5054779 | None:intergenic | 25.0% |
| AACTATAGGGATCTTGATAT+AGG | + | chr3.1:5055550-5055569 | MS.gene56278:CDS | 30.0% | |
| AATCCAACAAGCATATAAAG+TGG | + | chr3.1:5055464-5055483 | MS.gene56278:CDS | 30.0% | |
| ACATACAATATATGGTCAAG+TGG | + | chr3.1:5055422-5055441 | MS.gene56278:CDS | 30.0% | |
| AGAATAGTTGCTCAAAATAG+TGG | + | chr3.1:5054998-5055017 | MS.gene56278:CDS | 30.0% | |
| AGCTGCATATTACAACTATA+GGG | + | chr3.1:5055537-5055556 | MS.gene56278:CDS | 30.0% | |
| ATACTATGCAGCAAAAAGAT+TGG | + | chr3.1:5053759-5053778 | MS.gene56278:CDS | 30.0% | |
| ATTCAAAGAACATTGGAACA+AGG | + | chr3.1:5054911-5054930 | MS.gene56278:CDS | 30.0% | |
| CAAGATTAATGTCGATCAAA+CGG | - | chr3.1:5053878-5053897 | None:intergenic | 30.0% | |
| CAATACTTGTGGATACAATA+TGG | - | chr3.1:5053646-5053665 | None:intergenic | 30.0% | |
| CATACAATATATGGTCAAGT+GGG | + | chr3.1:5055423-5055442 | MS.gene56278:CDS | 30.0% | |
| CATATTGTATCCACAAGTAT+TGG | + | chr3.1:5053644-5053663 | MS.gene56278:CDS | 30.0% | |
| CTCACCTTATAGATGTAAAT+GGG | + | chr3.1:5054753-5054772 | MS.gene56278:CDS | 30.0% | |
| GATGAGATCAATCATGATAA+AGG | - | chr3.1:5053850-5053869 | None:intergenic | 30.0% | |
| TATTCAGCAAGAGTTCTATA+GGG | - | chr3.1:5055198-5055217 | None:intergenic | 30.0% | |
| TGAATTTCTGATTCATGAGA+AGG | - | chr3.1:5053732-5053751 | None:intergenic | 30.0% | |
| TTGAATACTGTAACAATAGG+TGG | - | chr3.1:5054890-5054909 | None:intergenic | 30.0% | |
| ! | AATCTTTTTGCTGCATAGTA+TGG | - | chr3.1:5053760-5053779 | None:intergenic | 30.0% |
| ! | CTCATTCCTGAAAAAGTTTT+GGG | - | chr3.1:5055683-5055702 | None:intergenic | 30.0% |
| ! | CTCTTTTACATAGTCAGATT+TGG | - | chr3.1:5055251-5055270 | None:intergenic | 30.0% |
| ! | TACAAGTTTCCTTTTCTATG+TGG | - | chr3.1:5055400-5055419 | None:intergenic | 30.0% |
| ! | TCATTCCTGAAAAAGTTTTG+GGG | - | chr3.1:5055682-5055701 | None:intergenic | 30.0% |
| ! | TCTAAAGCAAGTAAAGTTCA+TGG | + | chr3.1:5053957-5053976 | MS.gene56278:CDS | 30.0% |
| ! | TTCCTTTTCTATGTGGAAAA+GGG | - | chr3.1:5055393-5055412 | None:intergenic | 30.0% |
| ! | TTTCCTTTTCTATGTGGAAA+AGG | - | chr3.1:5055394-5055413 | None:intergenic | 30.0% |
| !! | ACTTATTTCTATAAGCTCTC+TGG | + | chr3.1:5054118-5054137 | MS.gene56278:intron | 30.0% |
| !! | CAATCAGTTTTGTATTTTGC+AGG | + | chr3.1:5055157-5055176 | MS.gene56278:CDS | 30.0% |
| !!! | AATGGGAGAAGATGTTTTTT+GGG | + | chr3.1:5054796-5054815 | MS.gene56278:CDS | 30.0% |
| AAGACAGTCCTTCATAATCA+TGG | - | chr3.1:5053812-5053831 | None:intergenic | 35.0% | |
| AAGGAAGAGAGAGTTAAACT+TGG | - | chr3.1:5055047-5055066 | None:intergenic | 35.0% | |
| AAGTGGATGAGAATGCTTTA+TGG | + | chr3.1:5055481-5055500 | MS.gene56278:CDS | 35.0% | |
| AAGTTTAACTCTCTCTTCCT+TGG | + | chr3.1:5055046-5055065 | MS.gene56278:CDS | 35.0% | |
| AATGAGAGTTTCCCTGAATT+AGG | + | chr3.1:5055097-5055116 | MS.gene56278:CDS | 35.0% | |
| AGAGAGAGTTAAACTTGGTT+TGG | - | chr3.1:5055042-5055061 | None:intergenic | 35.0% | |
| ATGACTGCATTGAAATGAGT+TGG | + | chr3.1:5055131-5055150 | MS.gene56278:CDS | 35.0% | |
| ATGAGAGTTTCCCTGAATTA+GGG | + | chr3.1:5055098-5055117 | MS.gene56278:CDS | 35.0% | |
| CACTGAAACAAAATCCAAGA+TGG | + | chr3.1:5053670-5053689 | MS.gene56278:CDS | 35.0% | |
| CATCCACTTTATATGCTTGT+TGG | - | chr3.1:5055470-5055489 | None:intergenic | 35.0% | |
| CTATTCAGCAAGAGTTCTAT+AGG | - | chr3.1:5055199-5055218 | None:intergenic | 35.0% | |
| GAGCTGCATATTACAACTAT+AGG | + | chr3.1:5055536-5055555 | MS.gene56278:CDS | 35.0% | |
| GCAACAAGTATGACAATACA+AGG | + | chr3.1:5055575-5055594 | MS.gene56278:CDS | 35.0% | |
| GCTCACCTTATAGATGTAAA+TGG | + | chr3.1:5054752-5054771 | MS.gene56278:CDS | 35.0% | |
| TAAGGGAAACTTTAAGCGAT+TGG | + | chr3.1:5055633-5055652 | MS.gene56278:CDS | 35.0% | |
| TAGTTGTAATATGCAGCTCT+TGG | - | chr3.1:5055535-5055554 | None:intergenic | 35.0% | |
| TCATTCTTGCATGGAAGATA+AGG | + | chr3.1:5054852-5054871 | MS.gene56278:CDS | 35.0% | |
| TTCAGAAAAGCTACTACCTA+TGG | - | chr3.1:5053589-5053608 | None:intergenic | 35.0% | |
| TTCAGTGATTCCAATACTTG+TGG | - | chr3.1:5053657-5053676 | None:intergenic | 35.0% | |
| ! | ATCCCTTTTCCACATAGAAA+AGG | + | chr3.1:5055388-5055407 | MS.gene56278:CDS | 35.0% |
| ! | CTTATAATGGAACCTTATGG+TGG | + | chr3.1:5055337-5055356 | MS.gene56278:CDS | 35.0% |
| ! | GATAAACACTGTGTTTTCAC+TGG | - | chr3.1:5053613-5053632 | None:intergenic | 35.0% |
| ! | GCTCATTCCTGAAAAAGTTT+TGG | - | chr3.1:5055684-5055703 | None:intergenic | 35.0% |
| !! | AAGCTGGTTTAGAAGGTATT+TGG | + | chr3.1:5055281-5055300 | MS.gene56278:CDS | 35.0% |
| !! | ATATGTCCAAGTGTTGGAAT+AGG | + | chr3.1:5054656-5054675 | MS.gene56278:intron | 35.0% |
| !! | CTTGGAGACTTTGAAACATA+AGG | - | chr3.1:5055517-5055536 | None:intergenic | 35.0% |
| !! | TTGATCCCCAAAACTTTTTC+AGG | + | chr3.1:5055674-5055693 | MS.gene56278:CDS | 35.0% |
| !!! | CAATGGGAGAAGATGTTTTT+TGG | + | chr3.1:5054795-5054814 | MS.gene56278:CDS | 35.0% |
| !!! | GATGTTTTTTGGGCTATTAG+AGG | + | chr3.1:5054806-5054825 | MS.gene56278:CDS | 35.0% |
| !!! | GTTTTGGAATCATTCTTGCA+TGG | + | chr3.1:5054843-5054862 | MS.gene56278:CDS | 35.0% |
| ACAGGTATATGTCCAAGTGT+TGG | + | chr3.1:5054650-5054669 | MS.gene56278:intron | 40.0% | |
| AGCTCAAACTAATTACCTGC+AGG | - | chr3.1:5053987-5054006 | None:intergenic | 40.0% | |
| ATACCTTCTAAACCAGCTTC+TGG | - | chr3.1:5055280-5055299 | None:intergenic | 40.0% | |
| ATGCAGTGCATGTTAACCAT+AGG | + | chr3.1:5053570-5053589 | MS.gene56278:CDS | 40.0% | |
| CACTTGGACATATACCTGTT+TGG | - | chr3.1:5054649-5054668 | None:intergenic | 40.0% | |
| CTATGTATTGCCACCTATGA+AGG | - | chr3.1:5054949-5054968 | None:intergenic | 40.0% | |
| CTGAATCCAAGCAGATTCAT+CGG | - | chr3.1:5053901-5053920 | None:intergenic | 40.0% | |
| CTTGATGAATTCAGCCATCT+TGG | - | chr3.1:5053687-5053706 | None:intergenic | 40.0% | |
| GCTCAAACTAATTACCTGCA+GGG | - | chr3.1:5053986-5054005 | None:intergenic | 40.0% | |
| GTACTCAGAAGCAAGTGTTT+GGG | + | chr3.1:5055597-5055616 | MS.gene56278:CDS | 40.0% | |
| TACTCAGAAGCAAGTGTTTG+GGG | + | chr3.1:5055598-5055617 | MS.gene56278:CDS | 40.0% | |
| TAGACCATGTTTCCTTACCA+AGG | - | chr3.1:5054711-5054730 | None:intergenic | 40.0% | |
| TCATTCAGTCTTCCACCATA+AGG | - | chr3.1:5055352-5055371 | None:intergenic | 40.0% | |
| TCTAAACCAGCTTCTGGTAT+AGG | - | chr3.1:5055274-5055293 | None:intergenic | 40.0% | |
| TCTTGCATGGAAGATAAGGT+TGG | + | chr3.1:5054856-5054875 | MS.gene56278:CDS | 40.0% | |
| TGTCTTGCAACCCTAATTCA+GGG | - | chr3.1:5055111-5055130 | None:intergenic | 40.0% | |
| TTGTCTTGCAACCCTAATTC+AGG | - | chr3.1:5055112-5055131 | None:intergenic | 40.0% | |
| ! | ATACCAGAAGCTGGTTTAGA+AGG | + | chr3.1:5055274-5055293 | MS.gene56278:CDS | 40.0% |
| ! | TGGGGCATCAAGTACTTTAA+GGG | + | chr3.1:5055616-5055635 | MS.gene56278:CDS | 40.0% |
| ! | TTGGGGCATCAAGTACTTTA+AGG | + | chr3.1:5055615-5055634 | MS.gene56278:CDS | 40.0% |
| !! | GAAGGTGTCATAATGAGAAG+AGG | - | chr3.1:5053714-5053733 | None:intergenic | 40.0% |
| !!! | GGCTTTGAATGAGCTTTTGA+AGG | - | chr3.1:5055230-5055249 | None:intergenic | 40.0% |
| !!! | GTTTTTTGGGCTATTAGAGG+AGG | + | chr3.1:5054809-5054828 | MS.gene56278:CDS | 40.0% |
| AAAGAGCCTATACCAGAAGC+TGG | + | chr3.1:5055265-5055284 | MS.gene56278:CDS | 45.0% | |
| AAAGTTCATGGATTCCCTGC+AGG | + | chr3.1:5053969-5053988 | MS.gene56278:CDS | 45.0% | |
| AGTGGTGGCCATGATTATGA+AGG | + | chr3.1:5053801-5053820 | MS.gene56278:CDS | 45.0% | |
| ATCTTGCCGATGAATCTGCT+TGG | + | chr3.1:5053892-5053911 | MS.gene56278:CDS | 45.0% | |
| ATTCAGGCTGGTGCAACATT+AGG | + | chr3.1:5053915-5053934 | MS.gene56278:CDS | 45.0% | |
| ATTGGAACAAGGAGCAACCA+AGG | + | chr3.1:5054922-5054941 | MS.gene56278:CDS | 45.0% | |
| CAGATTAGAGTGGTTAGTGG+TGG | + | chr3.1:5053786-5053805 | MS.gene56278:CDS | 45.0% | |
| CGATGAATCTGCTTGGATTC+AGG | + | chr3.1:5053899-5053918 | MS.gene56278:CDS | 45.0% | |
| GATTGGTGTGCAGATTAGAG+TGG | + | chr3.1:5053776-5053795 | MS.gene56278:CDS | 45.0% | |
| GGTACCTTGGTAAGGAAACA+TGG | + | chr3.1:5054704-5054723 | MS.gene56278:CDS | 45.0% | |
| GGTACTCAGAAGCAAGTGTT+TGG | + | chr3.1:5055596-5055615 | MS.gene56278:CDS | 45.0% | |
| GTGCAGATTAGAGTGGTTAG+TGG | + | chr3.1:5053783-5053802 | MS.gene56278:CDS | 45.0% | |
| TGACCACCTATTCCAACACT+TGG | - | chr3.1:5054665-5054684 | None:intergenic | 45.0% | |
| ! | GTGGATTTGGTACCTTGGTA+AGG | + | chr3.1:5054696-5054715 | MS.gene56278:CDS | 45.0% |
| !! | GGAGGTAGTGCTACTAGTTT+TGG | + | chr3.1:5054827-5054846 | MS.gene56278:CDS | 45.0% |
| !! | TGTCCAAGTGTTGGAATAGG+TGG | + | chr3.1:5054659-5054678 | MS.gene56278:intron | 45.0% |
| ATAGGTGGTCACATCAGTGG+AGG | + | chr3.1:5054674-5054693 | MS.gene56278:intron | 50.0% | |
| CAACCAAGGTCCTTCATAGG+TGG | + | chr3.1:5054936-5054955 | MS.gene56278:CDS | 50.0% | |
| GAATCTGCTTGGATTCAGGC+TGG | + | chr3.1:5053903-5053922 | MS.gene56278:CDS | 50.0% | |
| GAGCAACCAAGGTCCTTCAT+AGG | + | chr3.1:5054933-5054952 | MS.gene56278:CDS | 50.0% | |
| GGAATAGGTGGTCACATCAG+TGG | + | chr3.1:5054671-5054690 | MS.gene56278:intron | 50.0% | |
| TTGCCACCTATGAAGGACCT+TGG | - | chr3.1:5054942-5054961 | None:intergenic | 50.0% | |
| ! | CACATCAGTGGAGGTGGATT+TGG | + | chr3.1:5054683-5054702 | MS.gene56278:CDS | 50.0% |
| ! | TGGAGGTGGATTTGGTACCT+TGG | + | chr3.1:5054691-5054710 | MS.gene56278:CDS | 50.0% |
| CAGGGGAGGGAATTCAGAAG+AGG | - | chr3.1:5055715-5055734 | None:intergenic | 55.0% | |
| GGTGGTCACATCAGTGGAGG+TGG | + | chr3.1:5054677-5054696 | MS.gene56278:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 5053570 | 5055735 | 5053570 | ID=MS.gene56278 |
| chr3.1 | mRNA | 5053570 | 5055735 | 5053570 | ID=MS.gene56278.t1;Parent=MS.gene56278 |
| chr3.1 | exon | 5053570 | 5053990 | 5053570 | ID=MS.gene56278.t1.exon1;Parent=MS.gene56278.t1 |
| chr3.1 | CDS | 5053570 | 5053990 | 5053570 | ID=cds.MS.gene56278.t1;Parent=MS.gene56278.t1 |
| chr3.1 | exon | 5054678 | 5055735 | 5054678 | ID=MS.gene56278.t1.exon2;Parent=MS.gene56278.t1 |
| chr3.1 | CDS | 5054678 | 5055735 | 5054678 | ID=cds.MS.gene56278.t1;Parent=MS.gene56278.t1 |
| Gene Sequence |
| Protein sequence |
