Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57674.t1 | XP_013448012.1 | 97.6 | 378 | 9 | 0 | 1 | 378 | 28 | 405 | 2.30E-219 | 771.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57674.t1 | Q9LI89 | 48.5 | 388 | 184 | 5 | 4 | 375 | 34 | 421 | 1.0e-95 | 351.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57674.t1 | A0A072TX07 | 97.6 | 378 | 9 | 0 | 1 | 378 | 28 | 405 | 1.6e-219 | 771.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene57674 | -0.803569 | 3.27E-49 | -1.69E-46 |
| MS.gene049281 | MS.gene57674 | 0.856813 | 2.50E-62 | -1.69E-46 |
| MS.gene049320 | MS.gene57674 | -0.811382 | 7.20E-51 | -1.69E-46 |
| MS.gene050170 | MS.gene57674 | -0.811227 | 7.78E-51 | -1.69E-46 |
| MS.gene050381 | MS.gene57674 | 0.821967 | 3.05E-53 | -1.69E-46 |
| MS.gene050496 | MS.gene57674 | -0.825921 | 3.60E-54 | -1.69E-46 |
| MS.gene050504 | MS.gene57674 | -0.827395 | 1.60E-54 | -1.69E-46 |
| MS.gene050577 | MS.gene57674 | -0.824902 | 6.28E-54 | -1.69E-46 |
| MS.gene050612 | MS.gene57674 | -0.813521 | 2.45E-51 | -1.69E-46 |
| MS.gene050675 | MS.gene57674 | -0.805128 | 1.55E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene57674 | -0.823806 | 1.14E-53 | -1.69E-46 |
| MS.gene050883 | MS.gene57674 | 0.845485 | 3.94E-59 | -1.69E-46 |
| MS.gene050986 | MS.gene57674 | -0.804304 | 2.30E-49 | -1.69E-46 |
| MS.gene051127 | MS.gene57674 | 0.830334 | 3.11E-55 | -1.69E-46 |
| MS.gene051138 | MS.gene57674 | 0.805454 | 1.32E-49 | -1.69E-46 |
| MS.gene051364 | MS.gene57674 | 0.840663 | 7.59E-58 | -1.69E-46 |
| MS.gene051366 | MS.gene57674 | 0.825557 | 4.39E-54 | -1.69E-46 |
| MS.gene051367 | MS.gene57674 | 0.807265 | 5.50E-50 | -1.69E-46 |
| MS.gene051422 | MS.gene57674 | 0.801651 | 8.14E-49 | -1.69E-46 |
| MS.gene052211 | MS.gene57674 | 0.810806 | 9.60E-51 | -1.69E-46 |
| MS.gene052482 | MS.gene57674 | -0.809561 | 1.78E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene57674 | -0.811805 | 5.83E-51 | -1.69E-46 |
| MS.gene053203 | MS.gene57674 | -0.802191 | 6.31E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene57674 | 0.835281 | 1.84E-56 | -1.69E-46 |
| MS.gene053549 | MS.gene57674 | 0.81585 | 7.48E-52 | -1.69E-46 |
| MS.gene053826 | MS.gene57674 | 0.819205 | 1.31E-52 | -1.69E-46 |
| MS.gene053887 | MS.gene57674 | 0.801017 | 1.10E-48 | -1.69E-46 |
| MS.gene054706 | MS.gene57674 | 0.806938 | 6.45E-50 | -1.69E-46 |
| MS.gene054708 | MS.gene57674 | 0.804802 | 1.81E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene57674 | -0.829703 | 4.44E-55 | -1.69E-46 |
| MS.gene054954 | MS.gene57674 | -0.810858 | 9.35E-51 | -1.69E-46 |
| MS.gene054959 | MS.gene57674 | 0.84542 | 4.11E-59 | -1.69E-46 |
| MS.gene055154 | MS.gene57674 | 0.830266 | 3.24E-55 | -1.69E-46 |
| MS.gene055362 | MS.gene57674 | -0.810411 | 1.17E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene57674 | -0.828656 | 7.96E-55 | -1.69E-46 |
| MS.gene05589 | MS.gene57674 | -0.81154 | 6.65E-51 | -1.69E-46 |
| MS.gene056218 | MS.gene57674 | 0.813515 | 2.46E-51 | -1.69E-46 |
| MS.gene056311 | MS.gene57674 | 0.803973 | 2.70E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene57674 | -0.828586 | 8.28E-55 | -1.69E-46 |
| MS.gene056692 | MS.gene57674 | -0.812656 | 3.80E-51 | -1.69E-46 |
| MS.gene056748 | MS.gene57674 | 0.829287 | 5.60E-55 | -1.69E-46 |
| MS.gene056856 | MS.gene57674 | 0.801359 | 9.35E-49 | -1.69E-46 |
| MS.gene056988 | MS.gene57674 | 0.80785 | 4.13E-50 | -1.69E-46 |
| MS.gene057261 | MS.gene57674 | 0.818006 | 2.46E-52 | -1.69E-46 |
| MS.gene058068 | MS.gene57674 | 0.813372 | 2.65E-51 | -1.69E-46 |
| MS.gene058099 | MS.gene57674 | 0.820687 | 6.01E-53 | -1.69E-46 |
| MS.gene058111 | MS.gene57674 | 0.819433 | 1.16E-52 | -1.69E-46 |
| MS.gene058268 | MS.gene57674 | 0.819478 | 1.14E-52 | -1.69E-46 |
| MS.gene058269 | MS.gene57674 | 0.811117 | 8.22E-51 | -1.69E-46 |
| MS.gene058334 | MS.gene57674 | 0.80472 | 1.89E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene57674 | -0.804166 | 2.46E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene57674 | -0.813227 | 2.85E-51 | -1.69E-46 |
| MS.gene058544 | MS.gene57674 | -0.813087 | 3.06E-51 | -1.69E-46 |
| MS.gene058603 | MS.gene57674 | 0.816524 | 5.29E-52 | -1.69E-46 |
| MS.gene059022 | MS.gene57674 | -0.821529 | 3.85E-53 | -1.69E-46 |
| MS.gene059023 | MS.gene57674 | -0.806128 | 9.56E-50 | -1.69E-46 |
| MS.gene059223 | MS.gene57674 | 0.802057 | 6.72E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene57674 | -0.82089 | 5.40E-53 | -1.69E-46 |
| MS.gene059756 | MS.gene57674 | -0.815314 | 9.85E-52 | -1.69E-46 |
| MS.gene059865 | MS.gene57674 | -0.809709 | 1.65E-50 | -1.69E-46 |
| MS.gene059953 | MS.gene57674 | 0.829211 | 5.85E-55 | -1.69E-46 |
| MS.gene059955 | MS.gene57674 | 0.817518 | 3.16E-52 | -1.69E-46 |
| MS.gene060595 | MS.gene57674 | 0.830839 | 2.34E-55 | -1.69E-46 |
| MS.gene060778 | MS.gene57674 | -0.814389 | 1.58E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene57674 | -0.848634 | 5.41E-60 | -1.69E-46 |
| MS.gene060982 | MS.gene57674 | -0.800618 | 1.32E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57674.t1 | MTR_7g029290 | 97.619 | 378 | 9 | 0 | 1 | 378 | 28 | 405 | 0.0 | 763 |
| MS.gene57674.t1 | MTR_6g023805 | 64.433 | 388 | 116 | 6 | 1 | 376 | 28 | 405 | 3.08e-168 | 476 |
| MS.gene57674.t1 | MTR_2g094093 | 39.420 | 345 | 201 | 6 | 32 | 374 | 24 | 362 | 2.02e-77 | 243 |
| MS.gene57674.t1 | MTR_4g108280 | 36.388 | 371 | 213 | 10 | 18 | 374 | 17 | 378 | 2.85e-71 | 228 |
| MS.gene57674.t1 | MTR_0345s0010 | 38.005 | 371 | 211 | 11 | 13 | 374 | 73 | 433 | 1.07e-70 | 228 |
| MS.gene57674.t1 | MTR_0105s0020 | 38.005 | 371 | 211 | 11 | 13 | 374 | 73 | 433 | 1.48e-70 | 228 |
| MS.gene57674.t1 | MTR_0105s0130 | 38.005 | 371 | 211 | 11 | 13 | 374 | 73 | 433 | 3.56e-70 | 226 |
| MS.gene57674.t1 | MTR_0105s0170 | 37.736 | 371 | 212 | 11 | 13 | 374 | 86 | 446 | 2.10e-69 | 225 |
| MS.gene57674.t1 | MTR_0496s0010 | 38.095 | 378 | 195 | 13 | 13 | 374 | 55 | 409 | 2.41e-69 | 224 |
| MS.gene57674.t1 | MTR_0105s0040 | 37.736 | 371 | 212 | 11 | 13 | 374 | 171 | 531 | 5.73e-69 | 226 |
| MS.gene57674.t1 | MTR_4g022260 | 37.037 | 378 | 205 | 13 | 13 | 374 | 55 | 415 | 1.96e-68 | 222 |
| MS.gene57674.t1 | MTR_8g468030 | 39.244 | 344 | 192 | 9 | 39 | 374 | 161 | 495 | 3.55e-68 | 223 |
| MS.gene57674.t1 | MTR_1330s0010 | 38.136 | 354 | 202 | 10 | 13 | 357 | 86 | 431 | 8.47e-68 | 221 |
| MS.gene57674.t1 | MTR_4g022400 | 35.890 | 365 | 217 | 9 | 18 | 374 | 57 | 412 | 1.13e-67 | 219 |
| MS.gene57674.t1 | MTR_5g039390 | 37.435 | 382 | 218 | 11 | 5 | 374 | 102 | 474 | 1.64e-67 | 221 |
| MS.gene57674.t1 | MTR_8g085600 | 27.303 | 304 | 200 | 10 | 5 | 299 | 55 | 346 | 4.28e-24 | 103 |
| MS.gene57674.t1 | MTR_4g114110 | 25.168 | 298 | 198 | 10 | 9 | 298 | 69 | 349 | 3.80e-19 | 89.0 |
| MS.gene57674.t1 | MTR_3g073640 | 25.652 | 230 | 156 | 5 | 31 | 254 | 46 | 266 | 1.21e-16 | 81.6 |
| MS.gene57674.t1 | MTR_7g095220 | 25.957 | 235 | 145 | 8 | 32 | 248 | 8 | 231 | 2.45e-15 | 76.6 |
| MS.gene57674.t1 | MTR_8g080370 | 24.471 | 331 | 213 | 12 | 14 | 325 | 33 | 345 | 9.47e-15 | 75.5 |
| MS.gene57674.t1 | MTR_3g085570 | 24.272 | 309 | 201 | 11 | 32 | 325 | 39 | 329 | 1.54e-13 | 71.6 |
| MS.gene57674.t1 | MTR_2g019230 | 23.776 | 286 | 197 | 9 | 32 | 304 | 56 | 333 | 1.40e-12 | 68.9 |
| MS.gene57674.t1 | MTR_2g008440 | 25.543 | 368 | 199 | 14 | 31 | 326 | 46 | 410 | 1.67e-12 | 68.9 |
| MS.gene57674.t1 | MTR_2g012790 | 29.091 | 165 | 101 | 3 | 138 | 292 | 109 | 267 | 2.99e-12 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57674.t1 | AT3G27150 | 51.671 | 389 | 172 | 5 | 4 | 376 | 34 | 422 | 5.10e-133 | 387 |
| MS.gene57674.t1 | AT3G27150 | 51.671 | 389 | 172 | 5 | 4 | 376 | 34 | 422 | 5.10e-133 | 387 |
| MS.gene57674.t1 | AT5G40680 | 47.757 | 379 | 192 | 4 | 4 | 376 | 37 | 415 | 1.98e-119 | 352 |
| MS.gene57674.t1 | AT5G60570 | 40.462 | 346 | 196 | 7 | 32 | 374 | 53 | 391 | 2.21e-73 | 234 |
| MS.gene57674.t1 | AT5G60570 | 40.462 | 346 | 196 | 7 | 32 | 374 | 53 | 391 | 2.21e-73 | 234 |
| MS.gene57674.t1 | AT5G60570 | 40.462 | 346 | 196 | 7 | 32 | 374 | 53 | 391 | 2.21e-73 | 234 |
| MS.gene57674.t1 | AT1G14330 | 39.650 | 343 | 192 | 9 | 39 | 374 | 105 | 439 | 1.55e-69 | 225 |
| MS.gene57674.t1 | AT2G02870 | 37.922 | 385 | 209 | 12 | 12 | 374 | 89 | 465 | 3.63e-67 | 219 |
| MS.gene57674.t1 | AT2G02870 | 37.922 | 385 | 209 | 12 | 12 | 374 | 89 | 465 | 3.63e-67 | 219 |
| MS.gene57674.t1 | AT2G02870 | 37.922 | 385 | 209 | 12 | 12 | 374 | 89 | 465 | 3.63e-67 | 219 |
| MS.gene57674.t1 | AT1G74510 | 37.571 | 354 | 189 | 9 | 41 | 374 | 108 | 449 | 1.45e-64 | 213 |
| MS.gene57674.t1 | AT1G74510 | 37.571 | 354 | 189 | 9 | 41 | 374 | 108 | 449 | 1.45e-64 | 213 |
| MS.gene57674.t1 | AT1G74510 | 37.571 | 354 | 189 | 9 | 41 | 374 | 108 | 449 | 1.45e-64 | 213 |
| MS.gene57674.t1 | AT1G26930 | 36.340 | 377 | 206 | 10 | 13 | 374 | 62 | 419 | 5.25e-64 | 210 |
| MS.gene57674.t1 | AT1G26930 | 30.769 | 377 | 175 | 12 | 13 | 374 | 62 | 367 | 1.46e-41 | 150 |
| MS.gene57674.t1 | AT1G55270 | 26.449 | 276 | 185 | 8 | 32 | 299 | 82 | 347 | 1.00e-20 | 93.6 |
| MS.gene57674.t1 | AT3G63220 | 23.810 | 315 | 195 | 13 | 32 | 328 | 15 | 302 | 2.75e-13 | 70.9 |
| MS.gene57674.t1 | AT3G63220 | 23.810 | 315 | 195 | 13 | 32 | 328 | 15 | 302 | 2.75e-13 | 70.9 |
| MS.gene57674.t1 | AT4G35120 | 28.241 | 216 | 139 | 6 | 31 | 234 | 29 | 240 | 3.21e-13 | 70.9 |
| MS.gene57674.t1 | AT3G63220 | 24.510 | 306 | 186 | 13 | 41 | 328 | 17 | 295 | 3.44e-13 | 70.5 |
| MS.gene57674.t1 | AT1G60570 | 25.574 | 305 | 197 | 10 | 10 | 299 | 2 | 291 | 2.22e-12 | 68.2 |
| MS.gene57674.t1 | AT1G55270 | 25.500 | 200 | 134 | 7 | 105 | 299 | 32 | 221 | 1.84e-11 | 65.1 |
| MS.gene57674.t1 | AT4G29370 | 29.500 | 200 | 118 | 8 | 17 | 204 | 14 | 202 | 2.36e-11 | 65.1 |
| MS.gene57674.t1 | AT1G22040 | 25.751 | 233 | 151 | 7 | 31 | 254 | 46 | 265 | 3.38e-11 | 65.1 |
| MS.gene57674.t1 | AT1G16250 | 22.074 | 376 | 258 | 10 | 32 | 378 | 12 | 381 | 4.17e-11 | 64.3 |
| MS.gene57674.t1 | AT1G16250 | 22.074 | 376 | 258 | 10 | 32 | 378 | 12 | 381 | 4.17e-11 | 64.3 |
| MS.gene57674.t1 | AT2G29830 | 21.446 | 401 | 270 | 15 | 1 | 375 | 1 | 382 | 4.49e-11 | 64.3 |
Find 75 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACACCTATAGCGAAATTT+TGG | 0.201938 | 7.4:+72258100 | MS.gene57674:CDS |
| AATTTGACGGACCTGTTATT+TGG | 0.224264 | 7.4:+72257961 | MS.gene57674:CDS |
| TCAGATTACAGCTTTGAATT+TGG | 0.229608 | 7.4:+72257888 | MS.gene57674:CDS |
| GGGTCAGAGTTATTTGTTTC+GGG | 0.243484 | 7.4:+72257933 | MS.gene57674:CDS |
| GACAAGAGATTCTATGTTAT+TGG | 0.250445 | 7.4:+72258215 | MS.gene57674:CDS |
| AGTTTCCAATGTTGCGATCT+TGG | 0.284283 | 7.4:-72257693 | None:intergenic |
| CTATTTGCTTCAGCTAGTTC+TGG | 0.294486 | 7.4:+72258044 | MS.gene57674:CDS |
| GCGGCGAGATCTACAAAATA+AGG | 0.302206 | 7.4:+72257754 | MS.gene57674:CDS |
| TTTCTCTTTGAATTCATATT+CGG | 0.325901 | 7.4:-72258170 | None:intergenic |
| AGCTGATGCACAAGGCGGTT+GGG | 0.354563 | 7.4:+72258475 | MS.gene57674:CDS |
| CAGAACTAGCTGAAGCAAAT+AGG | 0.366562 | 7.4:-72258043 | None:intergenic |
| GTTGAAAGACATTCCACTTT+CGG | 0.373291 | 7.4:+72258322 | MS.gene57674:CDS |
| TACAGCTTAATCATTTCATT+AGG | 0.374102 | 7.4:+72258645 | MS.gene57674:CDS |
| GGTGATGAGTTACTTGTTAT+CGG | 0.384004 | 7.4:+72258518 | MS.gene57674:CDS |
| CATTTCAATTTCTCACCCTC+AGG | 0.390524 | 7.4:-72258599 | None:intergenic |
| AGGGTCAGAGTTATTTGTTT+CGG | 0.390691 | 7.4:+72257932 | MS.gene57674:CDS |
| CTGTAATCTGATTGAATCCT+TGG | 0.399975 | 7.4:-72257876 | None:intergenic |
| GAATCATATCGCCAAATAAC+AGG | 0.403217 | 7.4:-72257972 | None:intergenic |
| ATTTGAAAGGGATCAATAAC+TGG | 0.407898 | 7.4:+72258426 | MS.gene57674:CDS |
| TGTTATTTGGCGATATGATT+CGG | 0.418939 | 7.4:+72257974 | MS.gene57674:CDS |
| AATTTCTCACCCTCAGGATC+AGG | 0.424534 | 7.4:-72258593 | None:intergenic |
| CGAAAGCAATTGGTGGGGTA+TGG | 0.427240 | 7.4:+72257824 | MS.gene57674:CDS |
| TACAAAATAAGGAAAGAGTT+AGG | 0.428564 | 7.4:+72257765 | MS.gene57674:CDS |
| TTCGCATAAAGAACGAGCTT+TGG | 0.431074 | 7.4:+72258553 | MS.gene57674:CDS |
| GGAATGTCTTTCAACATGTC+AGG | 0.452600 | 7.4:-72258314 | None:intergenic |
| TTTGAATTCATATTCGGTAA+AGG | 0.467260 | 7.4:-72258164 | None:intergenic |
| GCTGATGCACAAGGCGGTTG+GGG | 0.472323 | 7.4:+72258476 | MS.gene57674:CDS |
| CTGAGGGTGAGAAATTGAAA+TGG | 0.473233 | 7.4:+72258600 | MS.gene57674:CDS |
| TAGCTAGCGGCGAAAGCAAT+TGG | 0.476177 | 7.4:+72257814 | MS.gene57674:CDS |
| AGCTTCCTGCTTGATTTGAA+AGG | 0.476416 | 7.4:-72257852 | None:intergenic |
| CGAGCTAGAGACATCGATCT+TGG | 0.476661 | 7.4:+72257662 | MS.gene57674:CDS |
| ATTCTATGTTATTGGTGGAC+AGG | 0.479496 | 7.4:+72258223 | MS.gene57674:CDS |
| AGCTGAAAGTGTATTTGAAA+GGG | 0.490575 | 7.4:+72258414 | MS.gene57674:CDS |
| AATCAAGCAGGAAGCTTCCA+AGG | 0.494763 | 7.4:+72257859 | MS.gene57674:CDS |
| GAGCTGAAAGTGTATTTGAA+AGG | 0.497856 | 7.4:+72258413 | MS.gene57674:CDS |
| TAGGAATTGTGCTGTGATGA+TGG | 0.506085 | 7.4:+72258664 | MS.gene57674:CDS |
| GAGCTGATGCACAAGGCGGT+TGG | 0.506566 | 7.4:+72258474 | MS.gene57674:CDS |
| GATTTCCAAGATCGCAACAT+TGG | 0.509774 | 7.4:+72257688 | MS.gene57674:CDS |
| CCTTGTGCATCAGCTCTCAC+AGG | 0.510167 | 7.4:-72258467 | None:intergenic |
| AAATGGAAGCAAATTGATTG+TGG | 0.518481 | 7.4:+72258617 | MS.gene57674:CDS |
| GAAAGAAGAATCTTTACCTC+AGG | 0.525784 | 7.4:+72257611 | MS.gene57674:CDS |
| GTTTCGGGTAAAGAATTTGA+CGG | 0.527787 | 7.4:+72257948 | MS.gene57674:CDS |
| GGTGTCCAAAATTTCGCTAT+AGG | 0.532541 | 7.4:-72258105 | None:intergenic |
| TACTTGTTGTCCTGATCCTG+AGG | 0.533715 | 7.4:+72258583 | MS.gene57674:CDS |
| AAGAGATTCTATGTTATTGG+TGG | 0.534243 | 7.4:+72258218 | MS.gene57674:CDS |
| GATACTAATGAATGGTTCAA+AGG | 0.541527 | 7.4:+72257996 | MS.gene57674:CDS |
| ATGATTCGGATACTAATGAA+TGG | 0.555866 | 7.4:+72257988 | MS.gene57674:CDS |
| TGAAGAACCGTTGCCATCAG+AGG | 0.562265 | 7.4:-72257586 | None:intergenic |
| TCGACGAAGAAACAAACACT+TGG | 0.563005 | 7.4:+72258282 | MS.gene57674:CDS |
| GTCCGTTCATGATTAATCCT+AGG | 0.572154 | 7.4:+72258018 | MS.gene57674:CDS |
| GCAATTGGTGGGGTATGGAA+TGG | 0.578887 | 7.4:+72257829 | MS.gene57674:CDS |
| TAGCGGCGAAAGCAATTGGT+GGG | 0.579866 | 7.4:+72257818 | MS.gene57674:CDS |
| TTACCACCCTCTGATGGCAA+CGG | 0.580464 | 7.4:+72257579 | MS.gene57674:CDS |
| CGTTCTTTATGCGAAACAGA+AGG | 0.588464 | 7.4:-72258545 | None:intergenic |
| GAAGAACCGTTGCCATCAGA+GGG | 0.591725 | 7.4:-72257585 | None:intergenic |
| TATTAACAACAGCTATCAGA+GGG | 0.594157 | 7.4:-72258358 | None:intergenic |
| GGAGAGTGCGAATCCGAAAG+TGG | 0.596088 | 7.4:-72258335 | None:intergenic |
| ACTTGTTGTCCTGATCCTGA+GGG | 0.606724 | 7.4:+72258584 | MS.gene57674:CDS |
| GGTGTTGCATTTAAATCACT+TGG | 0.608539 | 7.4:+72258497 | MS.gene57674:CDS |
| GACTTGTTACCACCCTCTGA+TGG | 0.609485 | 7.4:+72257573 | MS.gene57674:CDS |
| CACCTAGGATTAATCATGAA+CGG | 0.612535 | 7.4:-72258020 | None:intergenic |
| AATGGCCTTTCAAATCAAGC+AGG | 0.615937 | 7.4:+72257847 | MS.gene57674:CDS |
| AGGAATTGTGCTGTGATGAT+GGG | 0.616810 | 7.4:+72258665 | MS.gene57674:CDS |
| CTAGCGGCGAAAGCAATTGG+TGG | 0.628757 | 7.4:+72257817 | MS.gene57674:CDS |
| TTATTAACAACAGCTATCAG+AGG | 0.633695 | 7.4:-72258359 | None:intergenic |
| CCTGTGAGAGCTGATGCACA+AGG | 0.635244 | 7.4:+72258467 | MS.gene57674:CDS |
| GCTGAAGCAAATAGGCACCT+AGG | 0.638194 | 7.4:-72258035 | None:intergenic |
| AGCGGCGAAAGCAATTGGTG+GGG | 0.663533 | 7.4:+72257819 | MS.gene57674:CDS |
| AGATTATAATCTGCATCCTG+AGG | 0.665618 | 7.4:-72257627 | None:intergenic |
| GAACCGTTGCCATCAGAGGG+TGG | 0.673262 | 7.4:-72257582 | None:intergenic |
| ATTAACAACAGCTATCAGAG+GGG | 0.676676 | 7.4:-72258357 | None:intergenic |
| TGGACAGGACGAGAATCGAA+AGG | 0.677851 | 7.4:+72258238 | MS.gene57674:CDS |
| TTTCGAATCATATTTCTCAG+CGG | 0.689567 | 7.4:-72258126 | None:intergenic |
| TTAACAACAGCTATCAGAGG+GGG | 0.692839 | 7.4:-72258356 | None:intergenic |
| GTGAGAGCTGATGCACAAGG+CGG | 0.771409 | 7.4:+72258470 | MS.gene57674:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAATAACTGGAAAAAATT+AGG | + | chr7.4:72258437-72258456 | MS.gene57674:CDS | 20.0% |
| !! | TTTCTCTTTGAATTCATATT+CGG | - | chr7.4:72258173-72258192 | None:intergenic | 20.0% |
| ! | ATGATTCGAAAACAAAAACT+TGG | + | chr7.4:72258138-72258157 | MS.gene57674:CDS | 25.0% |
| ! | TACAAAATAAGGAAAGAGTT+AGG | + | chr7.4:72257765-72257784 | MS.gene57674:CDS | 25.0% |
| ! | TACAGCTTAATCATTTCATT+AGG | + | chr7.4:72258645-72258664 | MS.gene57674:CDS | 25.0% |
| ! | TTTGAATTCATATTCGGTAA+AGG | - | chr7.4:72258167-72258186 | None:intergenic | 25.0% |
| !! | ATAAAGAATCATTTTCAGCA+GGG | + | chr7.4:72257913-72257932 | MS.gene57674:CDS | 25.0% |
| !! | TCAAAGAGAAAATTTTGTTC+AGG | + | chr7.4:72258182-72258201 | MS.gene57674:CDS | 25.0% |
| AAATGGAAGCAAATTGATTG+TGG | + | chr7.4:72258617-72258636 | MS.gene57674:CDS | 30.0% | |
| AAGAGATTCTATGTTATTGG+TGG | + | chr7.4:72258218-72258237 | MS.gene57674:CDS | 30.0% | |
| ATTTGAAAGGGATCAATAAC+TGG | + | chr7.4:72258426-72258445 | MS.gene57674:CDS | 30.0% | |
| GACAAGAGATTCTATGTTAT+TGG | + | chr7.4:72258215-72258234 | MS.gene57674:CDS | 30.0% | |
| GATACTAATGAATGGTTCAA+AGG | + | chr7.4:72257996-72258015 | MS.gene57674:CDS | 30.0% | |
| TATTAACAACAGCTATCAGA+GGG | - | chr7.4:72258361-72258380 | None:intergenic | 30.0% | |
| TTATTAACAACAGCTATCAG+AGG | - | chr7.4:72258362-72258381 | None:intergenic | 30.0% | |
| TTTCGAATCATATTTCTCAG+CGG | - | chr7.4:72258129-72258148 | None:intergenic | 30.0% | |
| ! | AAACACCTATAGCGAAATTT+TGG | + | chr7.4:72258100-72258119 | MS.gene57674:CDS | 30.0% |
| ! | AGCTGAAAGTGTATTTGAAA+GGG | + | chr7.4:72258414-72258433 | MS.gene57674:CDS | 30.0% |
| ! | ATGATTCGGATACTAATGAA+TGG | + | chr7.4:72257988-72258007 | MS.gene57674:CDS | 30.0% |
| ! | ATTTTGTTCAGGATGTTACA+TGG | + | chr7.4:72258193-72258212 | MS.gene57674:CDS | 30.0% |
| ! | GATAAAGAATCATTTTCAGC+AGG | + | chr7.4:72257912-72257931 | MS.gene57674:CDS | 30.0% |
| ! | TGTTATTTGGCGATATGATT+CGG | + | chr7.4:72257974-72257993 | MS.gene57674:CDS | 30.0% |
| ! | TTTTTAACCCTGATGAAAAG+CGG | + | chr7.4:72257735-72257754 | MS.gene57674:CDS | 30.0% |
| !! | TCAGATTACAGCTTTGAATT+TGG | + | chr7.4:72257888-72257907 | MS.gene57674:CDS | 30.0% |
| AGATTATAATCTGCATCCTG+AGG | - | chr7.4:72257630-72257649 | None:intergenic | 35.0% | |
| AGGGTCAGAGTTATTTGTTT+CGG | + | chr7.4:72257932-72257951 | MS.gene57674:CDS | 35.0% | |
| ATTAACAACAGCTATCAGAG+GGG | - | chr7.4:72258360-72258379 | None:intergenic | 35.0% | |
| CACCTAGGATTAATCATGAA+CGG | - | chr7.4:72258023-72258042 | None:intergenic | 35.0% | |
| CTAGCTAACATAAAAACGGA+CGG | - | chr7.4:72257801-72257820 | None:intergenic | 35.0% | |
| CTGTAATCTGATTGAATCCT+TGG | - | chr7.4:72257879-72257898 | None:intergenic | 35.0% | |
| GAAAGAAGAATCTTTACCTC+AGG | + | chr7.4:72257611-72257630 | MS.gene57674:CDS | 35.0% | |
| GAATCATATCGCCAAATAAC+AGG | - | chr7.4:72257975-72257994 | None:intergenic | 35.0% | |
| GTTGAAAGACATTCCACTTT+CGG | + | chr7.4:72258322-72258341 | MS.gene57674:CDS | 35.0% | |
| ! | AATTTGACGGACCTGTTATT+TGG | + | chr7.4:72257961-72257980 | MS.gene57674:CDS | 35.0% |
| ! | ATTCTATGTTATTGGTGGAC+AGG | + | chr7.4:72258223-72258242 | MS.gene57674:CDS | 35.0% |
| ! | GAGCTGAAAGTGTATTTGAA+AGG | + | chr7.4:72258413-72258432 | MS.gene57674:CDS | 35.0% |
| ! | GGTGTTGCATTTAAATCACT+TGG | + | chr7.4:72258497-72258516 | MS.gene57674:CDS | 35.0% |
| ! | GTTTCGGGTAAAGAATTTGA+CGG | + | chr7.4:72257948-72257967 | MS.gene57674:CDS | 35.0% |
| !! | AATTTTGCTTTTGTTGCAGG+TGG | + | chr7.4:72258068-72258087 | MS.gene57674:CDS | 35.0% |
| !! | GGTAATTTTGCTTTTGTTGC+AGG | + | chr7.4:72258065-72258084 | MS.gene57674:CDS | 35.0% |
| !! | GGTGATGAGTTACTTGTTAT+CGG | + | chr7.4:72258518-72258537 | MS.gene57674:CDS | 35.0% |
| !! | TCCGTTTTTATGTTAGCTAG+CGG | + | chr7.4:72257801-72257820 | MS.gene57674:CDS | 35.0% |
| AATGGCCTTTCAAATCAAGC+AGG | + | chr7.4:72257847-72257866 | MS.gene57674:CDS | 40.0% | |
| AGGAATTGTGCTGTGATGAT+GGG | + | chr7.4:72258665-72258684 | MS.gene57674:CDS | 40.0% | |
| AGTTTCCAATGTTGCGATCT+TGG | - | chr7.4:72257696-72257715 | None:intergenic | 40.0% | |
| CAGAACTAGCTGAAGCAAAT+AGG | - | chr7.4:72258046-72258065 | None:intergenic | 40.0% | |
| CATTTCAATTTCTCACCCTC+AGG | - | chr7.4:72258602-72258621 | None:intergenic | 40.0% | |
| CGTTCTTTATGCGAAACAGA+AGG | - | chr7.4:72258548-72258567 | None:intergenic | 40.0% | |
| CTATTTGCTTCAGCTAGTTC+TGG | + | chr7.4:72258044-72258063 | MS.gene57674:CDS | 40.0% | |
| CTGAGGGTGAGAAATTGAAA+TGG | + | chr7.4:72258600-72258619 | MS.gene57674:CDS | 40.0% | |
| GATTTCCAAGATCGCAACAT+TGG | + | chr7.4:72257688-72257707 | MS.gene57674:CDS | 40.0% | |
| GCCGCTAGCTAACATAAAAA+CGG | - | chr7.4:72257805-72257824 | None:intergenic | 40.0% | |
| GGAATGTCTTTCAACATGTC+AGG | - | chr7.4:72258317-72258336 | None:intergenic | 40.0% | |
| GGGTCAGAGTTATTTGTTTC+GGG | + | chr7.4:72257933-72257952 | MS.gene57674:CDS | 40.0% | |
| GGTGTCCAAAATTTCGCTAT+AGG | - | chr7.4:72258108-72258127 | None:intergenic | 40.0% | |
| GTCCGTTCATGATTAATCCT+AGG | + | chr7.4:72258018-72258037 | MS.gene57674:CDS | 40.0% | |
| TAGGAATTGTGCTGTGATGA+TGG | + | chr7.4:72258664-72258683 | MS.gene57674:CDS | 40.0% | |
| TCGACGAAGAAACAAACACT+TGG | + | chr7.4:72258282-72258301 | MS.gene57674:CDS | 40.0% | |
| TTAACAACAGCTATCAGAGG+GGG | - | chr7.4:72258359-72258378 | None:intergenic | 40.0% | |
| TTCGCATAAAGAACGAGCTT+TGG | + | chr7.4:72258553-72258572 | MS.gene57674:CDS | 40.0% | |
| ! | AGCTTCCTGCTTGATTTGAA+AGG | - | chr7.4:72257855-72257874 | None:intergenic | 40.0% |
| AATCAAGCAGGAAGCTTCCA+AGG | + | chr7.4:72257859-72257878 | MS.gene57674:CDS | 45.0% | |
| AATTTCTCACCCTCAGGATC+AGG | - | chr7.4:72258596-72258615 | None:intergenic | 45.0% | |
| GCGGCGAGATCTACAAAATA+AGG | + | chr7.4:72257754-72257773 | MS.gene57674:CDS | 45.0% | |
| ! | TGCTTTTGTTGCAGGTGGTT+TGG | + | chr7.4:72258073-72258092 | MS.gene57674:CDS | 45.0% |
| !! | ACTTGTTGTCCTGATCCTGA+GGG | + | chr7.4:72258584-72258603 | MS.gene57674:CDS | 45.0% |
| !! | TACTTGTTGTCCTGATCCTG+AGG | + | chr7.4:72258583-72258602 | MS.gene57674:CDS | 45.0% |
| CGAAAGCAATTGGTGGGGTA+TGG | + | chr7.4:72257824-72257843 | MS.gene57674:CDS | 50.0% | |
| CGAGCTAGAGACATCGATCT+TGG | + | chr7.4:72257662-72257681 | MS.gene57674:CDS | 50.0% | |
| GAAGAACCGTTGCCATCAGA+GGG | - | chr7.4:72257588-72257607 | None:intergenic | 50.0% | |
| GCAATTGGTGGGGTATGGAA+TGG | + | chr7.4:72257829-72257848 | MS.gene57674:CDS | 50.0% | |
| GCTGAAGCAAATAGGCACCT+AGG | - | chr7.4:72258038-72258057 | None:intergenic | 50.0% | |
| TAGCGGCGAAAGCAATTGGT+GGG | + | chr7.4:72257818-72257837 | MS.gene57674:CDS | 50.0% | |
| TAGCTAGCGGCGAAAGCAAT+TGG | + | chr7.4:72257814-72257833 | MS.gene57674:CDS | 50.0% | |
| TGAAGAACCGTTGCCATCAG+AGG | - | chr7.4:72257589-72257608 | None:intergenic | 50.0% | |
| TGGACAGGACGAGAATCGAA+AGG | + | chr7.4:72258238-72258257 | MS.gene57674:CDS | 50.0% | |
| TTACCACCCTCTGATGGCAA+CGG | + | chr7.4:72257579-72257598 | MS.gene57674:CDS | 50.0% | |
| !! | GACTTGTTACCACCCTCTGA+TGG | + | chr7.4:72257573-72257592 | MS.gene57674:CDS | 50.0% |
| !!! | AGATCTCGCCGCTTTTCATC+AGG | - | chr7.4:72257746-72257765 | None:intergenic | 50.0% |
| !!! | GATCTCGCCGCTTTTCATCA+GGG | - | chr7.4:72257745-72257764 | None:intergenic | 50.0% |
| AGCGGCGAAAGCAATTGGTG+GGG | + | chr7.4:72257819-72257838 | MS.gene57674:CDS | 55.0% | |
| AGCTGATGCACAAGGCGGTT+GGG | + | chr7.4:72258475-72258494 | MS.gene57674:CDS | 55.0% | |
| CCTGTGAGAGCTGATGCACA+AGG | + | chr7.4:72258467-72258486 | MS.gene57674:CDS | 55.0% | |
| CCTTGTGCATCAGCTCTCAC+AGG | - | chr7.4:72258470-72258489 | None:intergenic | 55.0% | |
| CTAGCGGCGAAAGCAATTGG+TGG | + | chr7.4:72257817-72257836 | MS.gene57674:CDS | 55.0% | |
| GTGAGAGCTGATGCACAAGG+CGG | + | chr7.4:72258470-72258489 | MS.gene57674:CDS | 55.0% | |
| ! | GGAGAGTGCGAATCCGAAAG+TGG | - | chr7.4:72258338-72258357 | None:intergenic | 55.0% |
| GAACCGTTGCCATCAGAGGG+TGG | - | chr7.4:72257585-72257604 | None:intergenic | 60.0% | |
| GAGCTGATGCACAAGGCGGT+TGG | + | chr7.4:72258474-72258493 | MS.gene57674:CDS | 60.0% | |
| GCTGATGCACAAGGCGGTTG+GGG | + | chr7.4:72258476-72258495 | MS.gene57674:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 72257561 | 72258697 | 72257561 | ID=MS.gene57674 |
| chr7.4 | mRNA | 72257561 | 72258697 | 72257561 | ID=MS.gene57674.t1;Parent=MS.gene57674 |
| chr7.4 | exon | 72257561 | 72258697 | 72257561 | ID=MS.gene57674.t1.exon1;Parent=MS.gene57674.t1 |
| chr7.4 | CDS | 72257561 | 72258697 | 72257561 | ID=cds.MS.gene57674.t1;Parent=MS.gene57674.t1 |
| Gene Sequence |
| Protein sequence |
