AlfalfaGEDB | MS.gene58789

M. sativa cultivar XinJiangDaYe / MS.gene58789

KEGG: K13260
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0008150 GO:0008152 GO:0016491 GO:0016705 GO:0020037 GO:0043167 GO:0043169 GO:0046872 GO:0046906 GO:0046914 GO:0048037 GO:0055114 GO:0097159 GO:1901363
PFAM: PF00067.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58789.t1	XP_013463333.1	94.4	144	8	0	1	144	159	302	8.80E-71	276.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58789.t1	Q6WNQ9	93.8	144	9	0	1	144	157	300	1.8e-72	273.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58789.t1	A0A072V5R8	94.4	144	8	0	1	144	159	302	6.3e-71	276.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049150	MS.gene58789	0.840339	9.23E-58	-1.69E-46
MS.gene049151	MS.gene58789	0.818724	1.69E-52	-1.69E-46
MS.gene049153	MS.gene58789	0.836774	7.68E-57	-1.69E-46
MS.gene049577	MS.gene58789	0.801698	7.96E-49	-1.69E-46
MS.gene050060	MS.gene58789	0.849229	3.70E-60	-1.69E-46
MS.gene050133	MS.gene58789	-0.813415	2.59E-51	-1.69E-46
MS.gene050426	MS.gene58789	-0.831597	1.53E-55	-1.69E-46
MS.gene050642	MS.gene58789	0.839157	1.87E-57	-1.69E-46
MS.gene050680	MS.gene58789	0.804077	2.57E-49	-1.69E-46
MS.gene051373	MS.gene58789	0.84273	2.16E-58	-1.69E-46
MS.gene051535	MS.gene58789	0.802912	4.48E-49	-1.69E-46
MS.gene051983	MS.gene58789	0.861737	8.31E-64	-1.69E-46
MS.gene052011	MS.gene58789	-0.809167	2.16E-50	-1.69E-46
MS.gene052077	MS.gene58789	0.833334	5.66E-56	-1.69E-46
MS.gene052176	MS.gene58789	0.805264	1.45E-49	-1.69E-46
MS.gene052188	MS.gene58789	0.824324	8.58E-54	-1.69E-46
MS.gene052340	MS.gene58789	0.842565	2.39E-58	-1.69E-46
MS.gene052389	MS.gene58789	0.802534	5.36E-49	-1.69E-46
MS.gene052469	MS.gene58789	0.814281	1.67E-51	-1.69E-46
MS.gene052590	MS.gene58789	0.805942	1.05E-49	-1.69E-46
MS.gene052758	MS.gene58789	0.807447	5.03E-50	-1.69E-46
MS.gene053073	MS.gene58789	0.806834	6.79E-50	-1.69E-46
MS.gene05316	MS.gene58789	0.809393	1.94E-50	-1.69E-46
MS.gene053330	MS.gene58789	0.83181	1.35E-55	-1.69E-46
MS.gene053494	MS.gene58789	0.843843	1.09E-58	-1.69E-46
MS.gene053928	MS.gene58789	0.835685	1.45E-56	-1.69E-46
MS.gene054102	MS.gene58789	0.803742	3.01E-49	-1.69E-46
MS.gene054183	MS.gene58789	0.828532	8.53E-55	-1.69E-46
MS.gene054349	MS.gene58789	0.848518	5.82E-60	-1.69E-46
MS.gene054556	MS.gene58789	0.806127	9.56E-50	-1.69E-46
MS.gene054930	MS.gene58789	0.835345	1.77E-56	-1.69E-46
MS.gene05510	MS.gene58789	0.802939	4.42E-49	-1.69E-46
MS.gene055367	MS.gene58789	0.823098	1.66E-53	-1.69E-46
MS.gene055539	MS.gene58789	0.805466	1.32E-49	-1.69E-46
MS.gene05553	MS.gene58789	0.877747	4.90E-69	-1.69E-46
MS.gene05556	MS.gene58789	0.810171	1.32E-50	-1.69E-46
MS.gene055701	MS.gene58789	0.854754	9.97E-62	-1.69E-46
MS.gene056233	MS.gene58789	0.818047	2.40E-52	-1.69E-46
MS.gene056659	MS.gene58789	0.817637	2.97E-52	-1.69E-46
MS.gene056723	MS.gene58789	0.830752	2.46E-55	-1.69E-46
MS.gene056899	MS.gene58789	-0.805461	1.32E-49	-1.69E-46
MS.gene056979	MS.gene58789	0.857823	1.25E-62	-1.69E-46
MS.gene057156	MS.gene58789	-0.805425	1.34E-49	-1.69E-46
MS.gene057396	MS.gene58789	0.867619	1.20E-65	-1.69E-46
MS.gene057825	MS.gene58789	0.800141	1.66E-48	-1.69E-46
MS.gene058002	MS.gene58789	0.805069	1.59E-49	-1.69E-46
MS.gene058142	MS.gene58789	0.871578	6.14E-67	-1.69E-46
MS.gene058276	MS.gene58789	0.82884	7.19E-55	-1.69E-46
MS.gene058435	MS.gene58789	0.819809	9.56E-53	-1.69E-46
MS.gene058949	MS.gene58789	0.817073	3.98E-52	-1.69E-46
MS.gene059011	MS.gene58789	0.843071	1.76E-58	-1.69E-46
MS.gene059161	MS.gene58789	0.803007	4.28E-49	-1.69E-46
MS.gene059247	MS.gene58789	0.86976	2.43E-66	-1.69E-46
MS.gene059285	MS.gene58789	0.843561	1.30E-58	-1.69E-46
MS.gene059420	MS.gene58789	0.80995	1.47E-50	-1.69E-46
MS.gene059609	MS.gene58789	0.808463	3.06E-50	-1.69E-46
MS.gene059710	MS.gene58789	0.805895	1.07E-49	-1.69E-46
MS.gene059788	MS.gene58789	0.84635	2.29E-59	-1.69E-46
MS.gene059894	MS.gene58789	0.801429	9.04E-49	-1.69E-46
MS.gene059899	MS.gene58789	0.803394	3.56E-49	-1.69E-46
MS.gene060083	MS.gene58789	0.814	1.93E-51	-1.69E-46
MS.gene060247	MS.gene58789	0.806435	8.24E-50	-1.69E-46
MS.gene060407	MS.gene58789	0.830593	2.69E-55	-1.69E-46
MS.gene061049	MS.gene58789	0.80579	1.13E-49	-1.69E-46
MS.gene061114	MS.gene58789	-0.830869	2.30E-55	-1.69E-46
MS.gene061159	MS.gene58789	0.856615	2.85E-62	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58789.t1	MTR_2g437880	94.444	144	8	0	1	144	159	302	1.53e-94	282
MS.gene58789.t1	MTR_5g016410	66.165	133	43	1	11	143	166	296	4.31e-56	182
MS.gene58789.t1	MTR_4g094775	59.542	131	51	1	12	142	167	295	1.00e-50	168
MS.gene58789.t1	MTR_4g095050	59.701	134	51	2	11	143	169	300	1.49e-49	166
MS.gene58789.t1	MTR_2g437840	55.303	132	57	1	12	143	167	296	4.05e-43	148
MS.gene58789.t1	MTR_4g035330	53.731	134	49	2	11	144	165	285	9.26e-41	142
MS.gene58789.t1	MTR_4g094772	52.632	133	50	2	12	144	169	288	5.45e-40	140
MS.gene58789.t1	MTR_5g016440	46.970	132	64	2	12	143	166	291	1.70e-33	122
MS.gene58789.t1	MTR_4g077210	36.842	133	80	2	12	143	172	301	2.72e-25	100
MS.gene58789.t1	MTR_7g087520	34.211	152	90	3	1	144	160	309	5.69e-18	79.7
MS.gene58789.t1	MTR_6g008650	34.783	115	73	1	7	121	177	289	1.73e-16	75.5
MS.gene58789.t1	MTR_6g008500	38.318	107	65	1	2	108	183	288	6.23e-16	73.6
MS.gene58789.t1	MTR_4g109470	32.653	147	92	4	1	143	154	297	2.55e-15	72.0
MS.gene58789.t1	MTR_3g025260	35.821	134	79	4	15	143	169	300	3.22e-15	71.6
MS.gene58789.t1	MTR_8g020940	33.913	115	71	3	17	128	173	285	8.21e-15	70.5
MS.gene58789.t1	MTR_6g008640	33.846	130	80	3	7	131	177	305	8.27e-15	70.5
MS.gene58789.t1	MTR_8g020960	34.188	117	72	3	17	130	174	288	1.11e-14	70.1
MS.gene58789.t1	MTR_8g020950	33.621	116	72	3	17	129	175	288	1.33e-14	69.7
MS.gene58789.t1	MTR_3g024520	35.644	101	64	1	15	115	169	268	1.85e-14	69.3
MS.gene58789.t1	MTR_3g025230	35.644	101	64	1	15	115	169	268	1.94e-14	69.3
MS.gene58789.t1	MTR_6g042610	35.514	107	66	2	11	117	175	278	3.26e-14	68.9
MS.gene58789.t1	MTR_6g042540	30.435	138	88	3	11	141	175	311	7.39e-14	67.8
MS.gene58789.t1	MTR_2g450040	35.965	114	66	2	2	111	165	275	1.12e-13	67.4
MS.gene58789.t1	MTR_5g018480	31.579	133	80	3	15	141	185	312	1.31e-13	66.6
MS.gene58789.t1	MTR_6g008620	31.618	136	83	3	15	143	189	321	2.76e-13	66.2
MS.gene58789.t1	MTR_4g088425	31.034	116	74	1	15	130	176	285	4.61e-13	65.5
MS.gene58789.t1	MTR_3g014880	33.028	109	72	1	7	115	178	285	5.92e-13	65.1
MS.gene58789.t1	MTR_0638s0020	31.618	136	81	5	1	133	90	216	1.15e-12	64.3
MS.gene58789.t1	MTR_6g008600	31.429	140	87	4	12	143	185	323	2.40e-12	63.5
MS.gene58789.t1	MTR_5g045250	33.600	125	74	2	15	136	194	312	5.17e-12	62.4
MS.gene58789.t1	MTR_8g091690	32.520	123	75	3	13	133	161	277	7.26e-12	62.0
MS.gene58789.t1	MTR_3g025250	37.037	81	50	1	35	115	3	82	8.52e-12	61.2
MS.gene58789.t1	MTR_6g008530	31.915	141	86	5	12	143	186	325	2.39e-11	60.5
MS.gene58789.t1	MTR_3g436390	29.060	117	71	3	1	111	159	269	5.68e-11	59.3
MS.gene58789.t1	MTR_4g018800	29.358	109	76	1	7	115	179	286	6.13e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58789.t1	AT4G37340	50.000	132	61	3	12	143	168	294	3.51e-36	130
MS.gene58789.t1	AT4G37370	48.462	130	61	3	12	141	168	291	1.28e-35	128
MS.gene58789.t1	AT1G66540	48.462	130	62	3	12	141	55	179	6.12e-34	122
MS.gene58789.t1	AT2G23220	47.368	133	64	3	11	143	183	309	7.30e-34	124
MS.gene58789.t1	AT3G28740	46.617	133	65	4	11	143	176	302	1.17e-32	120
MS.gene58789.t1	AT4G37310	47.727	132	65	3	14	143	170	299	1.97e-32	120
MS.gene58789.t1	AT4G37430	43.796	137	71	3	7	143	164	294	8.86e-32	118
MS.gene58789.t1	AT1G66540	47.200	125	61	3	17	141	1	120	2.67e-31	114
MS.gene58789.t1	AT5G36220	45.802	131	65	4	13	143	30	154	2.88e-31	114
MS.gene58789.t1	AT4G37360	45.926	135	68	3	7	141	163	292	9.97e-31	115
MS.gene58789.t1	AT5G36220	45.802	131	65	4	13	143	176	300	2.38e-30	114
MS.gene58789.t1	AT4G37320	43.609	133	69	3	11	143	168	294	8.02e-29	110
MS.gene58789.t1	AT4G37400	42.308	130	69	3	14	143	166	289	8.15e-29	110
MS.gene58789.t1	AT2G23190	40.602	133	73	3	11	143	212	338	2.80e-28	108
MS.gene58789.t1	AT4G37330	42.105	133	68	3	11	143	168	291	4.61e-28	107
MS.gene58789.t1	AT5G67310	40.845	142	74	3	4	144	174	306	5.17e-28	107
MS.gene58789.t1	AT5G57220	43.411	129	67	3	15	143	167	289	2.47e-27	105
MS.gene58789.t1	AT4G37410	43.077	130	68	3	14	143	168	291	4.73e-26	102
MS.gene58789.t1	AT5G10600	36.641	131	77	3	14	143	184	309	7.51e-19	82.0
MS.gene58789.t1	AT4G31940	37.681	138	75	4	2	128	159	296	1.61e-18	81.3
MS.gene58789.t1	AT1G01280	33.557	149	93	4	1	144	155	302	2.51e-17	77.8
MS.gene58789.t1	AT5G10610	34.884	129	79	2	15	143	170	293	1.37e-15	72.8
MS.gene58789.t1	AT4G31970	34.454	119	73	2	15	128	177	295	3.35e-15	71.6
MS.gene58789.t1	AT4G31950	33.824	136	80	3	15	140	165	300	5.05e-15	71.2
MS.gene58789.t1	AT5G07990	32.759	116	74	1	11	126	164	275	1.00e-14	70.5

Find 0 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATTCAGATCATTTAATCAA+AGG	-	15010:19713-19732	MS.gene58789:CDS	20.0%
!!!	GTTTTTTGATCTTGATAATT+TGG	-	15010:19547-19566	MS.gene58789:CDS	20.0%
!	AAATGATCTGAATAATACTC+AGG	+	15010:19707-19726	MS.gene58789:intergenic	25.0%
!	TGAAGAAGCTAAACAATTTA+GGG	-	15010:19457-19476	MS.gene58789:CDS	25.0%
!	TTGAAGAAGCTAAACAATTT+AGG	-	15010:19456-19475	MS.gene58789:CDS	25.0%
!	TTGTTAGGTGCTAATAATAA+GGG	-	15010:19503-19522	MS.gene58789:CDS	25.0%
!	TTTGTTAGGTGCTAATAATA+AGG	-	15010:19502-19521	MS.gene58789:CDS	25.0%
!!	AAAAAGATCTGATGCATTTT+TGG	-	15010:19592-19611	MS.gene58789:CDS	25.0%
!!	ATCTTGATAATTTGGAAAAG+AGG	-	15010:19555-19574	MS.gene58789:CDS	25.0%
	AGATCAAAAAACCTTAACAG+AGG	+	15010:19539-19558	MS.gene58789:intergenic	30.0%
	AGTGAGATTATGTCTTTGTT+AGG	-	15010:19488-19507	MS.gene58789:CDS	30.0%
	TGCTATGATGAGAATGATAT+CGG	-	15010:19394-19413	MS.gene58789:CDS	30.0%
!!	AAAAGGTATTATGGAGATGA+TGG	-	15010:19419-19438	MS.gene58789:CDS	30.0%
!!	ATATCGGGAAAAAGGTATTA+TGG	-	15010:19410-19429	MS.gene58789:CDS	30.0%
!!!	ATTGATCATCTTTTGAAGCT+TGG	-	15010:19671-19690	MS.gene58789:CDS	30.0%
	AGAGGAAATATTCGTAGTGA+TGG	-	15010:19638-19657	MS.gene58789:CDS	35.0%
	GCTATGATGAGAATGATATC+GGG	-	15010:19395-19414	MS.gene58789:CDS	35.0%
	TGAGAATGATATCGGGAAAA+AGG	-	15010:19402-19421	MS.gene58789:CDS	35.0%
!!	AAGATCTGATGCATTTTTGG+AGG	-	15010:19595-19614	MS.gene58789:CDS	35.0%
!!	AGATCTGATGCATTTTTGGA+GGG	-	15010:19596-19615	MS.gene58789:CDS	35.0%
!!!	GTGATTTTTTGCCTCTGTTA+AGG	-	15010:19525-19544	MS.gene58789:CDS	35.0%
	AGGTTTCGGTTTCACTAAAG+TGG	-	15010:19337-19356	MS.gene58789:CDS	40.0%
	GGAAATATTCGTAGTGATGG+AGG	-	15010:19641-19660	MS.gene58789:CDS	40.0%
!!	ATTTTTGGAGGGACTCATTG+AGG	-	15010:19607-19626	MS.gene58789:CDS	40.0%
	CTCAAAGACGTCGTCTCTGA+AGG	-	15010:19317-19336	MS.gene58789:CDS	50.0%
	CTCATTGAGGAACATCGCAG+AGG	-	15010:19620-19639	MS.gene58789:CDS	50.0%
!	GACGTCGTCTCTGAAGGTTT+CGG	-	15010:19323-19342	MS.gene58789:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
15010	gene	19314	19745	19314	ID=MS.gene58789
15010	mRNA	19314	19745	19314	ID=MS.gene58789.t1;Parent=MS.gene58789
15010	exon	19314	19745	19314	ID=MS.gene58789.t1.exon1;Parent=MS.gene58789.t1
15010	CDS	19314	19745	19314	ID=cds.MS.gene58789.t1;Parent=MS.gene58789.t1

Gene Sequence

>MS.gene58789

CTTCTCAAAGACGTCGTCTCTGAAGGTTTCGGTTTCACTAAAGTGGAACTGAGATCGAGACTAACAGAGATGACGTTTAATGCTATGATGAGAATGATATCGGGAAAAAGGTATTATGGAGATGATGGAGACGTGACAGATGTTGAAGAAGCTAAACAATTTAGGGAGATAATAAGTGAGATTATGTCTTTGTTAGGTGCTAATAATAAGGGTGATTTTTTGCCTCTGTTAAGGTTTTTTGATCTTGATAATTTGGAAAAGAGGTGCAAGAAGATTGCAAAAAGATCTGATGCATTTTTGGAGGGACTCATTGAGGAACATCGCAGAGGAAATATTCGTAGTGATGGAGGTACAATGATTGATCATCTTTTGAAGCTTGGTGAATCACAACCTGAGTATTATTCAGATCATTTAATCAAAGGTCTAATTCAG

Protein sequence

>MS.gene58789.t1

LLKDVVSEGFGFTKVELRSRLTEMTFNAMMRMISGKRYYGDDGDVTDVEEAKQFREIISEIMSLLGANNKGDFLPLLRFFDLDNLEKRCKKIAKRSDAFLEGLIEEHRRGNIRSDGGTMIDHLLKLGESQPEYYSDHLIKGLIQ