Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59294.t1 | XP_024641827.1 | 99.2 | 604 | 5 | 0 | 1 | 604 | 238 | 841 | 0.00E+00 | 1201 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59294.t1 | A0A396HMI3 | 99.2 | 604 | 5 | 0 | 1 | 604 | 238 | 841 | 0.0e+00 | 1201.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene59294 | 0.821406 | 4.11E-53 | -1.69E-46 |
| MS.gene049341 | MS.gene59294 | 0.802344 | 5.87E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene59294 | 0.837363 | 5.43E-57 | -1.69E-46 |
| MS.gene049404 | MS.gene59294 | 0.80783 | 4.17E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene59294 | 0.832318 | 1.01E-55 | -1.69E-46 |
| MS.gene049821 | MS.gene59294 | 0.817723 | 2.84E-52 | -1.69E-46 |
| MS.gene049850 | MS.gene59294 | 0.815686 | 8.14E-52 | -1.69E-46 |
| MS.gene049887 | MS.gene59294 | 0.813513 | 2.46E-51 | -1.69E-46 |
| MS.gene050020 | MS.gene59294 | 0.826901 | 2.10E-54 | -1.69E-46 |
| MS.gene050241 | MS.gene59294 | 0.807224 | 5.61E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene59294 | 0.886001 | 5.00E-72 | -1.69E-46 |
| MS.gene050808 | MS.gene59294 | 0.850479 | 1.65E-60 | -1.69E-46 |
| MS.gene050901 | MS.gene59294 | 0.822697 | 2.06E-53 | -1.69E-46 |
| MS.gene051102 | MS.gene59294 | 0.801521 | 8.66E-49 | -1.69E-46 |
| MS.gene051180 | MS.gene59294 | 0.813741 | 2.20E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene59294 | 0.813891 | 2.03E-51 | -1.69E-46 |
| MS.gene051279 | MS.gene59294 | 0.823276 | 1.51E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene59294 | 0.824793 | 6.66E-54 | -1.69E-46 |
| MS.gene05157 | MS.gene59294 | 0.849436 | 3.24E-60 | -1.69E-46 |
| MS.gene051664 | MS.gene59294 | 0.801293 | 9.64E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene59294 | 0.813604 | 2.35E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene59294 | 0.877809 | 4.67E-69 | -1.69E-46 |
| MS.gene051720 | MS.gene59294 | 0.880988 | 3.49E-70 | -1.69E-46 |
| MS.gene051885 | MS.gene59294 | 0.803247 | 3.82E-49 | -1.69E-46 |
| MS.gene052020 | MS.gene59294 | 0.800646 | 1.31E-48 | -1.69E-46 |
| MS.gene052133 | MS.gene59294 | 0.850567 | 1.56E-60 | -1.69E-46 |
| MS.gene052397 | MS.gene59294 | 0.804221 | 2.40E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene59294 | 0.865043 | 7.86E-65 | -1.69E-46 |
| MS.gene052665 | MS.gene59294 | 0.865087 | 7.62E-65 | -1.69E-46 |
| MS.gene052732 | MS.gene59294 | 0.852121 | 5.68E-61 | -1.69E-46 |
| MS.gene053144 | MS.gene59294 | 0.859359 | 4.37E-63 | -1.69E-46 |
| MS.gene053174 | MS.gene59294 | 0.827399 | 1.60E-54 | -1.69E-46 |
| MS.gene053226 | MS.gene59294 | 0.827234 | 1.75E-54 | -1.69E-46 |
| MS.gene053563 | MS.gene59294 | 0.82172 | 3.47E-53 | -1.69E-46 |
| MS.gene053581 | MS.gene59294 | 0.863825 | 1.89E-64 | -1.69E-46 |
| MS.gene053599 | MS.gene59294 | 0.825905 | 3.63E-54 | -1.69E-46 |
| MS.gene05364 | MS.gene59294 | 0.830097 | 3.56E-55 | -1.69E-46 |
| MS.gene054550 | MS.gene59294 | 0.834826 | 2.39E-56 | -1.69E-46 |
| MS.gene054584 | MS.gene59294 | 0.866575 | 2.58E-65 | -1.69E-46 |
| MS.gene054710 | MS.gene59294 | 0.814943 | 1.19E-51 | -1.69E-46 |
| MS.gene054791 | MS.gene59294 | 0.822751 | 2.00E-53 | -1.69E-46 |
| MS.gene055144 | MS.gene59294 | 0.836758 | 7.76E-57 | -1.69E-46 |
| MS.gene055465 | MS.gene59294 | 0.876018 | 1.95E-68 | -1.69E-46 |
| MS.gene055466 | MS.gene59294 | 0.868023 | 8.88E-66 | -1.69E-46 |
| MS.gene055467 | MS.gene59294 | 0.814888 | 1.22E-51 | -1.69E-46 |
| MS.gene055468 | MS.gene59294 | 0.875583 | 2.75E-68 | -1.69E-46 |
| MS.gene055469 | MS.gene59294 | 0.863861 | 1.84E-64 | -1.69E-46 |
| MS.gene055512 | MS.gene59294 | 0.827191 | 1.79E-54 | -1.69E-46 |
| MS.gene055537 | MS.gene59294 | 0.859926 | 2.95E-63 | -1.69E-46 |
| MS.gene055580 | MS.gene59294 | -0.846717 | 1.82E-59 | -1.69E-46 |
| MS.gene055890 | MS.gene59294 | 0.841367 | 4.96E-58 | -1.69E-46 |
| MS.gene055891 | MS.gene59294 | 0.865791 | 4.57E-65 | -1.69E-46 |
| MS.gene055926 | MS.gene59294 | 0.809941 | 1.48E-50 | -1.69E-46 |
| MS.gene056005 | MS.gene59294 | 0.833773 | 4.40E-56 | -1.69E-46 |
| MS.gene056043 | MS.gene59294 | -0.835159 | 1.97E-56 | -1.69E-46 |
| MS.gene05604 | MS.gene59294 | 0.800223 | 1.59E-48 | -1.69E-46 |
| MS.gene056136 | MS.gene59294 | 0.805265 | 1.45E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene59294 | 0.851636 | 7.80E-61 | -1.69E-46 |
| MS.gene056507 | MS.gene59294 | 0.81328 | 2.77E-51 | -1.69E-46 |
| MS.gene056553 | MS.gene59294 | 0.815079 | 1.11E-51 | -1.69E-46 |
| MS.gene056643 | MS.gene59294 | 0.801234 | 9.91E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene59294 | 0.806326 | 8.69E-50 | -1.69E-46 |
| MS.gene058087 | MS.gene59294 | 0.822587 | 2.19E-53 | -1.69E-46 |
| MS.gene058339 | MS.gene59294 | 0.817067 | 4.00E-52 | -1.69E-46 |
| MS.gene05847 | MS.gene59294 | 0.803289 | 3.74E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene59294 | 0.809594 | 1.75E-50 | -1.69E-46 |
| MS.gene058668 | MS.gene59294 | 0.805854 | 1.09E-49 | -1.69E-46 |
| MS.gene058852 | MS.gene59294 | 0.829876 | 4.03E-55 | -1.69E-46 |
| MS.gene058945 | MS.gene59294 | 0.815804 | 7.66E-52 | -1.69E-46 |
| MS.gene05894 | MS.gene59294 | 0.807667 | 4.52E-50 | -1.69E-46 |
| MS.gene059171 | MS.gene59294 | 0.81012 | 1.35E-50 | -1.69E-46 |
| MS.gene059725 | MS.gene59294 | 0.809165 | 2.16E-50 | -1.69E-46 |
| MS.gene05978 | MS.gene59294 | 0.802555 | 5.31E-49 | -1.69E-46 |
| MS.gene059937 | MS.gene59294 | 0.830383 | 3.03E-55 | -1.69E-46 |
| MS.gene060519 | MS.gene59294 | 0.844264 | 8.42E-59 | -1.69E-46 |
| MS.gene060587 | MS.gene59294 | 0.862546 | 4.69E-64 | -1.69E-46 |
| MS.gene060603 | MS.gene59294 | 0.802424 | 5.65E-49 | -1.69E-46 |
| MS.gene060884 | MS.gene59294 | 0.801294 | 9.64E-49 | -1.69E-46 |
| MS.gene061006 | MS.gene59294 | 0.830485 | 2.86E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59294.t1 | AT1G62870 | 59.106 | 604 | 217 | 7 | 1 | 601 | 183 | 759 | 0.0 | 723 |
| MS.gene59294.t1 | AT1G12380 | 58.279 | 616 | 221 | 6 | 1 | 601 | 196 | 790 | 0.0 | 723 |
Find 117 sgRNAs with CRISPR-Local
Find 140 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGAGTTTGGTTAAGTTTA+TGG | 0.125038 | 6.4:-44107269 | MS.gene59294:CDS |
| GGCTAAGGCATTGAGGAATT+TGG | 0.155802 | 6.4:-44107086 | MS.gene59294:CDS |
| TAGGTCGATGTTTGCCTCTT+TGG | 0.199962 | 6.4:-44106592 | MS.gene59294:CDS |
| GATTGTTGCTGATAAGTTTA+AGG | 0.205077 | 6.4:-44107107 | MS.gene59294:CDS |
| TCGCTTGTGAAGATTATTAA+AGG | 0.212044 | 6.4:-44106650 | MS.gene59294:CDS |
| GTGAATCCGAGGCGAAAATT+AGG | 0.220507 | 6.4:-44107396 | MS.gene59294:CDS |
| TCTTTCATGAGAAAAGTTTC+TGG | 0.240470 | 6.4:-44106083 | MS.gene59294:CDS |
| TGTGATGGAGGACGCGTTTA+AGG | 0.241698 | 6.4:-44106759 | MS.gene59294:CDS |
| TTGTGTGAGTTTAAATCTTT+AGG | 0.280903 | 6.4:-44106167 | MS.gene59294:CDS |
| TTTGAAAAGGCGGTGTTTAC+CGG | 0.292873 | 6.4:-44107220 | MS.gene59294:CDS |
| TGTTTGAGACGCGAGGTTTC+CGG | 0.293572 | 6.4:-44107454 | MS.gene59294:CDS |
| CTTTGGATTTACTTGCTGAT+TGG | 0.300028 | 6.4:-44107570 | MS.gene59294:CDS |
| CTCTTAGTAGTCGACTTGTT+TGG | 0.307678 | 6.4:-44106199 | MS.gene59294:CDS |
| TTCTTCAACGGATCACATTT+CGG | 0.311143 | 6.4:+44106845 | None:intergenic |
| AGGTCGATGTTTGCCTCTTT+GGG | 0.314652 | 6.4:-44106591 | MS.gene59294:CDS |
| CCAGCCACTTCTCTAGCATT+TGG | 0.320989 | 6.4:+44106719 | None:intergenic |
| TGGTTCAGAATGAGGTGTTT+TGG | 0.325299 | 6.4:-44106694 | MS.gene59294:CDS |
| CGCGTTTAAGGCAATGTTCA+TGG | 0.335480 | 6.4:-44106747 | MS.gene59294:CDS |
| TCTTAGTAGTCGACTTGTTT+GGG | 0.336957 | 6.4:-44106198 | MS.gene59294:CDS |
| CATGTGGATTTAAGAGTAAT+TGG | 0.347188 | 6.4:-44106106 | MS.gene59294:CDS |
| AATAATGATAGGAGTTTGTA+TGG | 0.354700 | 6.4:-44107307 | MS.gene59294:CDS |
| TGTGTGAGTTTAAATCTTTA+GGG | 0.363967 | 6.4:-44106166 | MS.gene59294:CDS |
| GTGTTGTGGTGGTGAGAGTT+TGG | 0.367651 | 6.4:-44107281 | MS.gene59294:CDS |
| AGAACAGGTGAATTCTGCTT+TGG | 0.381175 | 6.4:-44107587 | MS.gene59294:CDS |
| GAAGTACCAGGTGAAGTCTT+AGG | 0.382034 | 6.4:+44107619 | None:intergenic |
| ATCTTCGTCACTTCCAGAAA+TGG | 0.395777 | 6.4:+44105941 | None:intergenic |
| GATTATTAAAGGAATGGTTC+AGG | 0.407422 | 6.4:-44106639 | MS.gene59294:CDS |
| AGAATGCAGGAGATGGAGTA+TGG | 0.418553 | 6.4:-44106890 | MS.gene59294:CDS |
| AATACTTCTTGTCAGCTTCA+AGG | 0.424656 | 6.4:-44107040 | MS.gene59294:CDS |
| GTAGTAATTATAATAATGAT+AGG | 0.432117 | 6.4:-44107318 | MS.gene59294:CDS |
| TGAATCCGAGGCGAAAATTA+GGG | 0.434677 | 6.4:-44107395 | MS.gene59294:CDS |
| CAGCATCATTTGATGTTGTC+TGG | 0.438103 | 6.4:-44107703 | None:intergenic |
| AGGGTTTGTTAGTTTAATCA+AGG | 0.438901 | 6.4:-44107020 | MS.gene59294:CDS |
| TACTTCTTTGAGTAAAGAAC+AGG | 0.443784 | 6.4:-44107602 | MS.gene59294:CDS |
| TGCTGATAAGTTTAAGGCTA+AGG | 0.450816 | 6.4:-44107101 | MS.gene59294:CDS |
| CCGGTCCAAGGCTCGATGCT+AGG | 0.455130 | 6.4:-44107435 | MS.gene59294:CDS |
| ATCTCTTTGTTTCATCTGAA+CGG | 0.455464 | 6.4:+44106253 | None:intergenic |
| TTTATGGTGAATCTACCAAA+TGG | 0.459003 | 6.4:-44107253 | MS.gene59294:CDS |
| ATACTTCTTGTCAGCTTCAA+GGG | 0.488138 | 6.4:-44107039 | MS.gene59294:CDS |
| AGTGATGGTTGGAAGAGTTA+TGG | 0.491295 | 6.4:-44107349 | MS.gene59294:CDS |
| TGTGAAGATTATTAAAGGAA+TGG | 0.492323 | 6.4:-44106645 | MS.gene59294:CDS |
| AAGGCGGTGTTTACCGGCGG+GGG | 0.493111 | 6.4:-44107214 | MS.gene59294:CDS |
| ATTTGGAGAATCAAAACCAT+TGG | 0.493435 | 6.4:-44107069 | MS.gene59294:CDS |
| GTGTCTTTAATCAAGTAAAG+TGG | 0.493780 | 6.4:+44106437 | None:intergenic |
| TGCTAGAGAAGTGGCTGGAA+TGG | 0.497321 | 6.4:-44106714 | MS.gene59294:CDS |
| ACTTTCATCTTACCAGTGAC+TGG | 0.498140 | 6.4:+44106230 | None:intergenic |
| GACGCGAGGTTTCCGGTCCA+AGG | 0.501975 | 6.4:-44107447 | MS.gene59294:CDS |
| GAGACTGTGACTGGTGTTAG+TGG | 0.502637 | 6.4:-44107154 | MS.gene59294:CDS |
| ACTACACCACTCCTTCACTT+TGG | 0.506556 | 6.4:+44106556 | None:intergenic |
| GAGGCGGAGAGGCCGTTGAT+AGG | 0.507472 | 6.4:-44106611 | MS.gene59294:CDS |
| AGGAATGGTTCAGGATATAG+AGG | 0.509857 | 6.4:-44106630 | MS.gene59294:CDS |
| ACATTAAGATTGCATTGCAT+CGG | 0.512574 | 6.4:+44105896 | None:intergenic |
| TCTCAAGTTGGTTTGCCTGT+AGG | 0.521067 | 6.4:-44107481 | MS.gene59294:CDS |
| AGTTTGTATGGTTTGTGTTG+TGG | 0.522198 | 6.4:-44107295 | MS.gene59294:CDS |
| TGCAGGAGATGGAGTATGGC+GGG | 0.525001 | 6.4:-44106886 | MS.gene59294:CDS |
| ATCCTCAAGCATCGGAAACA+CGG | 0.525442 | 6.4:+44106814 | None:intergenic |
| CAAACAAGTCGACTACTAAG+AGG | 0.527797 | 6.4:+44106200 | None:intergenic |
| TTCAAGTAGCTAGTGATGGT+TGG | 0.529096 | 6.4:-44107360 | MS.gene59294:CDS |
| GTGTCAGAAACTTGAATGGC+GGG | 0.529427 | 6.4:+44106399 | None:intergenic |
| TAATTATAGAATGCAGGAGA+TGG | 0.530457 | 6.4:-44106897 | MS.gene59294:CDS |
| AAAAGATGATTTATGTGCAT+TGG | 0.530765 | 6.4:-44107677 | MS.gene59294:CDS |
| TTAAACCTAGCATCGAGCCT+TGG | 0.532416 | 6.4:+44107430 | None:intergenic |
| ATAAATGCCGCTGACCATGC+AGG | 0.535049 | 6.4:+44106467 | None:intergenic |
| TGAACGGCTTGCGCATAAAG+AGG | 0.540575 | 6.4:+44106269 | None:intergenic |
| TCGCGTGTCAGAAACTTGAA+TGG | 0.541251 | 6.4:+44106395 | None:intergenic |
| CGTCTCAAACAACTTCCTAC+AGG | 0.542256 | 6.4:+44107466 | None:intergenic |
| AAGAAGTATTTACCATCCAA+TGG | 0.543075 | 6.4:+44107053 | None:intergenic |
| GTCCGTGTTTCCGATGCTTG+AGG | 0.545204 | 6.4:-44106816 | MS.gene59294:CDS |
| TTATGGTGAATCTACCAAAT+GGG | 0.546125 | 6.4:-44107252 | MS.gene59294:CDS |
| TTGTATGGTTTGTGTTGTGG+TGG | 0.548848 | 6.4:-44107292 | MS.gene59294:CDS |
| CCTAGCATCGAGCCTTGGAC+CGG | 0.553355 | 6.4:+44107435 | None:intergenic |
| AACGGATCACATTTCGGCGA+AGG | 0.553597 | 6.4:+44106851 | None:intergenic |
| CTAATGAAATGGAGATCAGA+AGG | 0.554981 | 6.4:-44106299 | MS.gene59294:CDS |
| TTTCTTCATGCAACTTCATG+TGG | 0.555413 | 6.4:-44106122 | MS.gene59294:CDS |
| AAAAGGCGGTGTTTACCGGC+GGG | 0.560565 | 6.4:-44107216 | MS.gene59294:CDS |
| GGAGAATCAAAACCATTGGA+TGG | 0.563446 | 6.4:-44107065 | MS.gene59294:CDS |
| AAACAAAGAGATCCAGTCAC+TGG | 0.567086 | 6.4:-44106242 | MS.gene59294:CDS |
| CCAAATGCTAGAGAAGTGGC+TGG | 0.568259 | 6.4:-44106719 | MS.gene59294:CDS |
| AGTTTAAGGCTAAGGCATTG+AGG | 0.568636 | 6.4:-44107093 | MS.gene59294:CDS |
| AGTGATTCAAATGGTTGTGA+TGG | 0.570710 | 6.4:-44106774 | MS.gene59294:CDS |
| TCTTTACTCAAAGAAGTACC+AGG | 0.573758 | 6.4:+44107607 | None:intergenic |
| AGTGTTGTTCAAAGATGTGT+AGG | 0.575113 | 6.4:-44107130 | MS.gene59294:CDS |
| GTTCTCATCTCCTCCCAAAG+AGG | 0.577317 | 6.4:+44106578 | None:intergenic |
| CTCAAAATATCCTCAAGCAT+CGG | 0.586264 | 6.4:+44106806 | None:intergenic |
| TCGATGTTTGCCTCTTTGGG+AGG | 0.587276 | 6.4:-44106588 | MS.gene59294:CDS |
| GGCTGGAATGGTTCAGAATG+AGG | 0.587957 | 6.4:-44106702 | MS.gene59294:CDS |
| TAAATGCCGCTGACCATGCA+GGG | 0.606286 | 6.4:+44106468 | None:intergenic |
| AGTAAATACAATGTCGTCGA+AGG | 0.607878 | 6.4:-44106536 | MS.gene59294:CDS |
| GGAGATGAGAACCAAAGTGA+AGG | 0.608611 | 6.4:-44106567 | MS.gene59294:CDS |
| GAATTCGAAGTATGCTGAGG+AGG | 0.608662 | 6.4:-44107185 | MS.gene59294:CDS |
| GAACGGCTTGCGCATAAAGA+GGG | 0.613633 | 6.4:+44106270 | None:intergenic |
| GTGTTGTTCAAAGATGTGTA+GGG | 0.614226 | 6.4:-44107129 | MS.gene59294:CDS |
| GATTCAAATGGTTGTGATGG+AGG | 0.614297 | 6.4:-44106771 | MS.gene59294:CDS |
| TGAGAACCAAAGTGAAGGAG+TGG | 0.616062 | 6.4:-44106562 | MS.gene59294:CDS |
| GAAAAGGCGGTGTTTACCGG+CGG | 0.617999 | 6.4:-44107217 | MS.gene59294:CDS |
| CAATGTCGTCGAAGGACCAG+TGG | 0.618270 | 6.4:-44106528 | MS.gene59294:CDS |
| GGCAAACATCGACCTATCAA+CGG | 0.623513 | 6.4:+44106599 | None:intergenic |
| AGGAAGTTGTTTGAGACGCG+AGG | 0.625523 | 6.4:-44107461 | MS.gene59294:CDS |
| GGATCCAAATGCTAGAGAAG+TGG | 0.625781 | 6.4:-44106723 | MS.gene59294:CDS |
| CTGTGCTAGAGTGATTCAAA+TGG | 0.626672 | 6.4:-44106783 | MS.gene59294:CDS |
| AAGAGTCCTAAGACTTCACC+TGG | 0.630400 | 6.4:-44107625 | MS.gene59294:CDS |
| TACTTGATTAAAGACACAAG+TGG | 0.632678 | 6.4:-44106431 | MS.gene59294:CDS |
| CATCATTTGATGTTGTCTGG+TGG | 0.636248 | 6.4:-44107700 | None:intergenic |
| AAAGGCGGTGTTTACCGGCG+GGG | 0.650088 | 6.4:-44107215 | MS.gene59294:CDS |
| TAAATCTTTAGGGAAGATAG+CGG | 0.650273 | 6.4:-44106156 | MS.gene59294:CDS |
| CGTGTCAGAAACTTGAATGG+CGG | 0.650834 | 6.4:+44106398 | None:intergenic |
| TGAAGTTAAGAGTGAATCCG+AGG | 0.651148 | 6.4:-44107407 | MS.gene59294:CDS |
| ACGGACGCAAAGTTCTTCAA+CGG | 0.658006 | 6.4:+44106833 | None:intergenic |
| CTACCACCCTGCATGGTCAG+CGG | 0.663349 | 6.4:-44106474 | MS.gene59294:CDS |
| TAGACAATGAATCTCAGGTG+AGG | 0.669241 | 6.4:-44106931 | MS.gene59294:CDS |
| ATGCAGGAGATGGAGTATGG+CGG | 0.678386 | 6.4:-44106887 | MS.gene59294:CDS |
| AATGGTTCAGGATATAGAGG+CGG | 0.695270 | 6.4:-44106627 | MS.gene59294:CDS |
| TTCAGGATATAGAGGCGGAG+AGG | 0.697045 | 6.4:-44106622 | MS.gene59294:CDS |
| GGTGAATTCGAAGTATGCTG+AGG | 0.699279 | 6.4:-44107188 | MS.gene59294:CDS |
| GTTTAAGGCAATGTTCATGG+AGG | 0.722672 | 6.4:-44106744 | MS.gene59294:CDS |
| CTTTAGGGAAGATAGCGGTG+AGG | 0.746883 | 6.4:-44106151 | MS.gene59294:CDS |
| ATGCCGCTGACCATGCAGGG+TGG | 0.746921 | 6.4:+44106471 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAGTAATTATAATAATGAT+AGG | - | chr6.4:44106272-44106291 | MS.gene59294:CDS | 15.0% |
| !!! | TTTTGTTAATTATAGAATGC+AGG | - | chr6.4:44106687-44106706 | MS.gene59294:CDS | 20.0% |
| ! | AAAAGATGATTTATGTGCAT+TGG | - | chr6.4:44105913-44105932 | MS.gene59294:CDS | 25.0% |
| ! | AATAATGATAGGAGTTTGTA+TGG | - | chr6.4:44106283-44106302 | MS.gene59294:CDS | 25.0% |
| ! | TGTGAAGATTATTAAAGGAA+TGG | - | chr6.4:44106945-44106964 | MS.gene59294:CDS | 25.0% |
| ! | TGTGTGAGTTTAAATCTTTA+GGG | - | chr6.4:44107424-44107443 | MS.gene59294:CDS | 25.0% |
| ! | TTGTGTGAGTTTAAATCTTT+AGG | - | chr6.4:44107423-44107442 | MS.gene59294:CDS | 25.0% |
| !! | TTTTATAGACAATGAATCTC+AGG | - | chr6.4:44106654-44106673 | MS.gene59294:CDS | 25.0% |
| ACTAGCTACTTGAAAAAACA+TGG | + | chr6.4:44106224-44106243 | None:intergenic | 30.0% | |
| ATTTGGAGAATCAAAACCAT+TGG | - | chr6.4:44106521-44106540 | MS.gene59294:CDS | 30.0% | |
| CATGTGGATTTAAGAGTAAT+TGG | - | chr6.4:44107484-44107503 | MS.gene59294:CDS | 30.0% | |
| GATTATTAAAGGAATGGTTC+AGG | - | chr6.4:44106951-44106970 | MS.gene59294:CDS | 30.0% | |
| GATTGTTGCTGATAAGTTTA+AGG | - | chr6.4:44106483-44106502 | MS.gene59294:CDS | 30.0% | |
| GTGTCTTTAATCAAGTAAAG+TGG | + | chr6.4:44107156-44107175 | None:intergenic | 30.0% | |
| TAATTATAGAATGCAGGAGA+TGG | - | chr6.4:44106693-44106712 | MS.gene59294:CDS | 30.0% | |
| TACTTCTTTGAGTAAAGAAC+AGG | - | chr6.4:44105988-44106007 | MS.gene59294:CDS | 30.0% | |
| TACTTGATTAAAGACACAAG+TGG | - | chr6.4:44107159-44107178 | MS.gene59294:CDS | 30.0% | |
| TCGCTTGTGAAGATTATTAA+AGG | - | chr6.4:44106940-44106959 | MS.gene59294:CDS | 30.0% | |
| TCTTTCATGAGAAAAGTTTC+TGG | - | chr6.4:44107507-44107526 | MS.gene59294:CDS | 30.0% | |
| TGAGAGTTTGGTTAAGTTTA+TGG | - | chr6.4:44106321-44106340 | MS.gene59294:CDS | 30.0% | |
| TGGAGAAAATACTCGAAAAA+CGG | - | chr6.4:44107082-44107101 | MS.gene59294:CDS | 30.0% | |
| TTATGGTGAATCTACCAAAT+GGG | - | chr6.4:44106338-44106357 | MS.gene59294:CDS | 30.0% | |
| TTTATGGTGAATCTACCAAA+TGG | - | chr6.4:44106337-44106356 | MS.gene59294:CDS | 30.0% | |
| ! | ATCTCTTTGTTTCATCTGAA+CGG | + | chr6.4:44107340-44107359 | None:intergenic | 30.0% |
| ! | TAAATCTTTAGGGAAGATAG+CGG | - | chr6.4:44107434-44107453 | MS.gene59294:CDS | 30.0% |
| ! | TTTTGATGGAGCTAATGAAA+TGG | - | chr6.4:44107280-44107299 | MS.gene59294:CDS | 30.0% |
| !! | AAGAAGTATTTACCATCCAA+TGG | + | chr6.4:44106540-44106559 | None:intergenic | 30.0% |
| !! | AGGGTTTGTTAGTTTAATCA+AGG | - | chr6.4:44106570-44106589 | MS.gene59294:CDS | 30.0% |
| !!! | GATTGGTTTTATGAATGTTG+TGG | - | chr6.4:44106037-44106056 | MS.gene59294:CDS | 30.0% |
| !!! | TTTTTTCAAGTAGCTAGTGA+TGG | - | chr6.4:44106226-44106245 | MS.gene59294:CDS | 30.0% |
| AATACTTCTTGTCAGCTTCA+AGG | - | chr6.4:44106550-44106569 | MS.gene59294:CDS | 35.0% | |
| AGTAAATACAATGTCGTCGA+AGG | - | chr6.4:44107054-44107073 | MS.gene59294:CDS | 35.0% | |
| AGTGATTCAAATGGTTGTGA+TGG | - | chr6.4:44106816-44106835 | MS.gene59294:CDS | 35.0% | |
| ATACTTCTTGTCAGCTTCAA+GGG | - | chr6.4:44106551-44106570 | MS.gene59294:CDS | 35.0% | |
| CTAATGAAATGGAGATCAGA+AGG | - | chr6.4:44107291-44107310 | MS.gene59294:CDS | 35.0% | |
| CTCAAAATATCCTCAAGCAT+CGG | + | chr6.4:44106787-44106806 | None:intergenic | 35.0% | |
| TCAAAAACACTACTCCCATT+TGG | + | chr6.4:44106355-44106374 | None:intergenic | 35.0% | |
| TTCTTCAACGGATCACATTT+CGG | + | chr6.4:44106748-44106767 | None:intergenic | 35.0% | |
| TTTCTTCATGCAACTTCATG+TGG | - | chr6.4:44107468-44107487 | MS.gene59294:CDS | 35.0% | |
| ! | AGTGTTGTTCAAAGATGTGT+AGG | - | chr6.4:44106460-44106479 | MS.gene59294:CDS | 35.0% |
| ! | AGTTTGTATGGTTTGTGTTG+TGG | - | chr6.4:44106295-44106314 | MS.gene59294:CDS | 35.0% |
| ! | CAAGCTTTTCTTTCTCAAGT+TGG | - | chr6.4:44106097-44106116 | MS.gene59294:CDS | 35.0% |
| ! | CTTTGGATTTACTTGCTGAT+TGG | - | chr6.4:44106020-44106039 | MS.gene59294:CDS | 35.0% |
| ! | GCAGAATTGTTTTCCATTTC+TGG | - | chr6.4:44107636-44107655 | MS.gene59294:CDS | 35.0% |
| ! | GTGTTGTTCAAAGATGTGTA+GGG | - | chr6.4:44106461-44106480 | MS.gene59294:CDS | 35.0% |
| ! | TGCTGATAAGTTTAAGGCTA+AGG | - | chr6.4:44106489-44106508 | MS.gene59294:CDS | 35.0% |
| !! | AAGAAGAGATTTTTCTAGCG+AGG | - | chr6.4:44107602-44107621 | MS.gene59294:CDS | 35.0% |
| !! | CAAAAACTCTAGAGTTGCAT+TGG | - | chr6.4:44107533-44107552 | MS.gene59294:CDS | 35.0% |
| !! | GAGTAGTGTTTTTGAAAAGG+CGG | - | chr6.4:44106360-44106379 | MS.gene59294:CDS | 35.0% |
| !! | TCTTAGTAGTCGACTTGTTT+GGG | - | chr6.4:44107392-44107411 | MS.gene59294:CDS | 35.0% |
| !! | TCTTTACTCAAAGAAGTACC+AGG | + | chr6.4:44105986-44106005 | None:intergenic | 35.0% |
| !! | TGGGAGTAGTGTTTTTGAAA+AGG | - | chr6.4:44106357-44106376 | MS.gene59294:CDS | 35.0% |
| !!! | TTTTCGAGTATTTTCTCCAC+TGG | + | chr6.4:44107081-44107100 | None:intergenic | 35.0% |
| AAACAAAGAGATCCAGTCAC+TGG | - | chr6.4:44107348-44107367 | MS.gene59294:CDS | 40.0% | |
| AATGGTTCAGGATATAGAGG+CGG | - | chr6.4:44106963-44106982 | MS.gene59294:CDS | 40.0% | |
| ACTTTCATCTTACCAGTGAC+TGG | + | chr6.4:44107363-44107382 | None:intergenic | 40.0% | |
| AGAACAGGTGAATTCTGCTT+TGG | - | chr6.4:44106003-44106022 | MS.gene59294:CDS | 40.0% | |
| AGGAATGGTTCAGGATATAG+AGG | - | chr6.4:44106960-44106979 | MS.gene59294:CDS | 40.0% | |
| AGTGATGGTTGGAAGAGTTA+TGG | - | chr6.4:44106241-44106260 | MS.gene59294:CDS | 40.0% | |
| ATCTTCGTCACTTCCAGAAA+TGG | + | chr6.4:44107652-44107671 | None:intergenic | 40.0% | |
| CAAACAAGTCGACTACTAAG+AGG | + | chr6.4:44107393-44107412 | None:intergenic | 40.0% | |
| CTGTGCTAGAGTGATTCAAA+TGG | - | chr6.4:44106807-44106826 | MS.gene59294:CDS | 40.0% | |
| GATTCAAATGGTTGTGATGG+AGG | - | chr6.4:44106819-44106838 | MS.gene59294:CDS | 40.0% | |
| GGAGAATCAAAACCATTGGA+TGG | - | chr6.4:44106525-44106544 | MS.gene59294:CDS | 40.0% | |
| TAGACAATGAATCTCAGGTG+AGG | - | chr6.4:44106659-44106678 | MS.gene59294:CDS | 40.0% | |
| TGAAGTTAAGAGTGAATCCG+AGG | - | chr6.4:44106183-44106202 | MS.gene59294:CDS | 40.0% | |
| TGAATCCGAGGCGAAAATTA+GGG | - | chr6.4:44106195-44106214 | MS.gene59294:CDS | 40.0% | |
| TTCAAGTAGCTAGTGATGGT+TGG | - | chr6.4:44106230-44106249 | MS.gene59294:CDS | 40.0% | |
| TTTGAAAAGGCGGTGTTTAC+CGG | - | chr6.4:44106370-44106389 | MS.gene59294:CDS | 40.0% | |
| ! | AACAACGAGCTCGAACTTTT+CGG | - | chr6.4:44106598-44106617 | MS.gene59294:CDS | 40.0% |
| ! | GTTTAAGGCAATGTTCATGG+AGG | - | chr6.4:44106846-44106865 | MS.gene59294:CDS | 40.0% |
| ! | TTGTATGGTTTGTGTTGTGG+TGG | - | chr6.4:44106298-44106317 | MS.gene59294:CDS | 40.0% |
| !! | AGTTTAAGGCTAAGGCATTG+AGG | - | chr6.4:44106497-44106516 | MS.gene59294:CDS | 40.0% |
| !! | CTCTTAGTAGTCGACTTGTT+TGG | - | chr6.4:44107391-44107410 | MS.gene59294:CDS | 40.0% |
| !! | TGGTTCAGAATGAGGTGTTT+TGG | - | chr6.4:44106896-44106915 | MS.gene59294:CDS | 40.0% |
| !!! | AGAAGCTCATGTCGTTTTGA+TGG | - | chr6.4:44107266-44107285 | MS.gene59294:CDS | 40.0% |
| AAGAGTCCTAAGACTTCACC+TGG | - | chr6.4:44105965-44105984 | MS.gene59294:CDS | 45.0% | |
| ACGGACGCAAAGTTCTTCAA+CGG | + | chr6.4:44106760-44106779 | None:intergenic | 45.0% | |
| ACTACACCACTCCTTCACTT+TGG | + | chr6.4:44107037-44107056 | None:intergenic | 45.0% | |
| AGAATGCAGGAGATGGAGTA+TGG | - | chr6.4:44106700-44106719 | MS.gene59294:CDS | 45.0% | |
| AGGTCGATGTTTGCCTCTTT+GGG | - | chr6.4:44106999-44107018 | MS.gene59294:CDS | 45.0% | |
| CGTCTCAAACAACTTCCTAC+AGG | + | chr6.4:44106127-44106146 | None:intergenic | 45.0% | |
| CGTGTCAGAAACTTGAATGG+CGG | + | chr6.4:44107195-44107214 | None:intergenic | 45.0% | |
| GAAAAAACTACCACCCTGCA+TGG | - | chr6.4:44107109-44107128 | MS.gene59294:CDS | 45.0% | |
| GAATTCGAAGTATGCTGAGG+AGG | - | chr6.4:44106405-44106424 | MS.gene59294:CDS | 45.0% | |
| GGAGATGAGAACCAAAGTGA+AGG | - | chr6.4:44107023-44107042 | MS.gene59294:CDS | 45.0% | |
| GGATCCAAATGCTAGAGAAG+TGG | - | chr6.4:44106867-44106886 | MS.gene59294:CDS | 45.0% | |
| GGCAAACATCGACCTATCAA+CGG | + | chr6.4:44106994-44107013 | None:intergenic | 45.0% | |
| GGTGAATTCGAAGTATGCTG+AGG | - | chr6.4:44106402-44106421 | MS.gene59294:CDS | 45.0% | |
| GTGAATCCGAGGCGAAAATT+AGG | - | chr6.4:44106194-44106213 | MS.gene59294:CDS | 45.0% | |
| GTGTCAGAAACTTGAATGGC+GGG | + | chr6.4:44107194-44107213 | None:intergenic | 45.0% | |
| TAGGTCGATGTTTGCCTCTT+TGG | - | chr6.4:44106998-44107017 | MS.gene59294:CDS | 45.0% | |
| TCGCGTGTCAGAAACTTGAA+TGG | + | chr6.4:44107198-44107217 | None:intergenic | 45.0% | |
| TGAGAACCAAAGTGAAGGAG+TGG | - | chr6.4:44107028-44107047 | MS.gene59294:CDS | 45.0% | |
| ! | AGTATGCTGAGGAGGTTTTG+TGG | - | chr6.4:44106413-44106432 | MS.gene59294:CDS | 45.0% |
| ! | CGCGTTTAAGGCAATGTTCA+TGG | - | chr6.4:44106843-44106862 | MS.gene59294:CDS | 45.0% |
| ! | GAAGTACCAGGTGAAGTCTT+AGG | + | chr6.4:44105974-44105993 | None:intergenic | 45.0% |
| ! | TTAAACCTAGCATCGAGCCT+TGG | + | chr6.4:44106163-44106182 | None:intergenic | 45.0% |
| !! | ATCCTCAAGCATCGGAAACA+CGG | + | chr6.4:44106779-44106798 | None:intergenic | 45.0% |
| !! | GGCTAAGGCATTGAGGAATT+TGG | - | chr6.4:44106504-44106523 | MS.gene59294:CDS | 45.0% |
| !! | GTATGCTGAGGAGGTTTTGT+GGG | - | chr6.4:44106414-44106433 | MS.gene59294:CDS | 45.0% |
| !! | TCTCAAGTTGGTTTGCCTGT+AGG | - | chr6.4:44106109-44106128 | MS.gene59294:CDS | 45.0% |
| !!! | GAATGAGGTGTTTTGGAACG+AGG | - | chr6.4:44106903-44106922 | MS.gene59294:CDS | 45.0% |
| !!! | GTTTTGGAACGAGGTTGAAG+CGG | - | chr6.4:44106912-44106931 | MS.gene59294:CDS | 45.0% |
| AACGGATCACATTTCGGCGA+AGG | + | chr6.4:44106742-44106761 | None:intergenic | 50.0% | |
| ATAAATGCCGCTGACCATGC+AGG | + | chr6.4:44107126-44107145 | None:intergenic | 50.0% | |
| ATGCAGGAGATGGAGTATGG+CGG | - | chr6.4:44106703-44106722 | MS.gene59294:CDS | 50.0% | |
| CCAAATGCTAGAGAAGTGGC+TGG | - | chr6.4:44106871-44106890 | MS.gene59294:CDS | 50.0% | |
| CCAGCCACTTCTCTAGCATT+TGG | + | chr6.4:44106874-44106893 | None:intergenic | 50.0% | |
| GAACGGCTTGCGCATAAAGA+GGG | + | chr6.4:44107323-44107342 | None:intergenic | 50.0% | |
| GGCTGGAATGGTTCAGAATG+AGG | - | chr6.4:44106888-44106907 | MS.gene59294:CDS | 50.0% | |
| GTTCTCATCTCCTCCCAAAG+AGG | + | chr6.4:44107015-44107034 | None:intergenic | 50.0% | |
| TAAATGCCGCTGACCATGCA+GGG | + | chr6.4:44107125-44107144 | None:intergenic | 50.0% | |
| TCGATGTTTGCCTCTTTGGG+AGG | - | chr6.4:44107002-44107021 | MS.gene59294:CDS | 50.0% | |
| TGAACGGCTTGCGCATAAAG+AGG | + | chr6.4:44107324-44107343 | None:intergenic | 50.0% | |
| TGCTAGAGAAGTGGCTGGAA+TGG | - | chr6.4:44106876-44106895 | MS.gene59294:CDS | 50.0% | |
| TTCAGGATATAGAGGCGGAG+AGG | - | chr6.4:44106968-44106987 | MS.gene59294:CDS | 50.0% | |
| ! | AGGAAGTTGTTTGAGACGCG+AGG | - | chr6.4:44106129-44106148 | MS.gene59294:CDS | 50.0% |
| ! | CTTTAGGGAAGATAGCGGTG+AGG | - | chr6.4:44107439-44107458 | MS.gene59294:CDS | 50.0% |
| ! | GAGACTGTGACTGGTGTTAG+TGG | - | chr6.4:44106436-44106455 | MS.gene59294:CDS | 50.0% |
| ! | GGCATCCCTAATTTTCGCCT+CGG | + | chr6.4:44106203-44106222 | None:intergenic | 50.0% |
| ! | GTGTTGTGGTGGTGAGAGTT+TGG | - | chr6.4:44106309-44106328 | MS.gene59294:CDS | 50.0% |
| ! | GTTTTGTGGGAGACTGTGAC+TGG | - | chr6.4:44106427-44106446 | MS.gene59294:CDS | 50.0% |
| ! | TGTGATGGAGGACGCGTTTA+AGG | - | chr6.4:44106831-44106850 | MS.gene59294:CDS | 50.0% |
| ! | TGTTTGAGACGCGAGGTTTC+CGG | - | chr6.4:44106136-44106155 | MS.gene59294:CDS | 50.0% |
| AAAAGGCGGTGTTTACCGGC+GGG | - | chr6.4:44106374-44106393 | MS.gene59294:CDS | 55.0% | |
| CAATGTCGTCGAAGGACCAG+TGG | - | chr6.4:44107062-44107081 | MS.gene59294:CDS | 55.0% | |
| GAAAAGGCGGTGTTTACCGG+CGG | - | chr6.4:44106373-44106392 | MS.gene59294:CDS | 55.0% | |
| GTCCGTGTTTCCGATGCTTG+AGG | - | chr6.4:44106774-44106793 | MS.gene59294:CDS | 55.0% | |
| TGCAGGAGATGGAGTATGGC+GGG | - | chr6.4:44106704-44106723 | MS.gene59294:CDS | 55.0% | |
| AAAGGCGGTGTTTACCGGCG+GGG | - | chr6.4:44106375-44106394 | MS.gene59294:CDS | 60.0% | |
| CTACCACCCTGCATGGTCAG+CGG | - | chr6.4:44107116-44107135 | MS.gene59294:CDS | 60.0% | |
| ! | CCTAGCATCGAGCCTTGGAC+CGG | + | chr6.4:44106158-44106177 | None:intergenic | 60.0% |
| AAGGCGGTGTTTACCGGCGG+GGG | - | chr6.4:44106376-44106395 | MS.gene59294:CDS | 65.0% | |
| ATGCCGCTGACCATGCAGGG+TGG | + | chr6.4:44107122-44107141 | None:intergenic | 65.0% | |
| CCGGTCCAAGGCTCGATGCT+AGG | - | chr6.4:44106155-44106174 | MS.gene59294:CDS | 65.0% | |
| CGAATTCACCACTCCCCCGC+CGG | + | chr6.4:44106392-44106411 | None:intergenic | 65.0% | |
| GACGCGAGGTTTCCGGTCCA+AGG | - | chr6.4:44106143-44106162 | MS.gene59294:CDS | 65.0% | |
| GAGGCGGAGAGGCCGTTGAT+AGG | - | chr6.4:44106979-44106998 | MS.gene59294:CDS | 65.0% | |
| GGTGTTTACCGGCGGGGGAG+TGG | - | chr6.4:44106381-44106400 | MS.gene59294:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 44105899 | 44107713 | 44105899 | ID=MS.gene59294 |
| chr6.4 | mRNA | 44105899 | 44107713 | 44105899 | ID=MS.gene59294.t1;Parent=MS.gene59294 |
| chr6.4 | exon | 44105899 | 44107713 | 44105899 | ID=MS.gene59294.t1.exon1;Parent=MS.gene59294.t1 |
| chr6.4 | CDS | 44105899 | 44107713 | 44105899 | ID=cds.MS.gene59294.t1;Parent=MS.gene59294.t1 |
| Gene Sequence |
| Protein sequence |
