AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59493

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59493.t1 MTR_5g022560 96.802 344 11 0 1 344 1 344 0.0 701
MS.gene59493.t1 MTR_4g079770 89.595 346 33 3 1 344 1 345 0.0 640
MS.gene59493.t1 MTR_4g107930 88.986 345 37 1 1 344 1 345 0.0 639
MS.gene59493.t1 MTR_4g047610 90.116 344 33 1 1 344 1 343 0.0 634
MS.gene59493.t1 MTR_4g079470 89.538 325 32 2 21 344 22 345 0.0 605
MS.gene59493.t1 MTR_4g079800 86.769 325 41 2 21 344 22 345 0.0 589
MS.gene59493.t1 MTR_2g075830 75.291 344 82 2 1 344 1 341 0.0 525
MS.gene59493.t1 MTR_4g080700 66.049 324 98 6 25 344 25 340 8.44e-156 441
MS.gene59493.t1 MTR_4g080730 62.751 349 116 7 1 344 1 340 3.28e-155 439
MS.gene59493.t1 MTR_4g080360 53.392 339 153 4 8 344 7 342 4.77e-132 380
MS.gene59493.t1 MTR_2g083930 53.709 337 143 4 12 342 13 342 3.89e-130 375
MS.gene59493.t1 MTR_4g104370 55.394 343 142 9 6 342 5 342 1.08e-128 372
MS.gene59493.t1 MTR_3g075390 53.079 341 144 7 14 342 7 343 6.27e-125 363
MS.gene59493.t1 MTR_4g079440 55.016 309 133 3 34 342 41 343 8.53e-122 354
MS.gene59493.t1 MTR_2g090890 51.176 340 152 6 10 343 5 336 6.44e-121 352
MS.gene59493.t1 MTR_7g078600 57.419 310 121 6 34 342 37 336 4.45e-120 350
MS.gene59493.t1 MTR_1g107550 54.658 322 130 8 32 342 38 354 4.41e-117 347
MS.gene59493.t1 MTR_3g116080 52.212 339 149 7 10 343 17 347 1.25e-116 342
MS.gene59493.t1 MTR_7g079230 50.585 342 161 6 1 342 1 334 3.35e-115 337
MS.gene59493.t1 MTR_4g094918 48.824 340 164 6 10 343 6 341 2.92e-114 336
MS.gene59493.t1 MTR_3g102220 51.376 327 141 7 27 342 41 360 1.20e-111 333
MS.gene59493.t1 MTR_1g018840 49.148 352 150 8 8 342 21 360 8.62e-111 330
MS.gene59493.t1 MTR_1g018840 49.148 352 150 8 8 342 21 360 1.31e-110 330
MS.gene59493.t1 MTR_2g015850 54.138 290 126 5 55 342 78 362 6.64e-100 299
MS.gene59493.t1 MTR_3g112430 49.518 311 144 5 40 343 37 341 1.66e-99 301
MS.gene59493.t1 MTR_2g016180 46.349 315 153 8 37 343 49 355 1.27e-86 270
MS.gene59493.t1 MTR_2g016270 46.474 312 142 8 37 343 49 340 7.58e-85 265
MS.gene59493.t1 MTR_2g067900 46.372 317 149 10 38 341 15 323 3.47e-80 248
MS.gene59493.t1 MTR_2g064475 42.120 349 147 16 38 343 15 351 3.32e-75 236
MS.gene59493.t1 MTR_2g067560 46.417 321 141 14 38 338 15 324 3.51e-75 249
MS.gene59493.t1 MTR_4g125300 41.234 308 164 10 41 343 53 348 1.07e-68 219
MS.gene59493.t1 MTR_2g015920 39.938 323 174 12 31 343 46 358 6.64e-68 218
MS.gene59493.t1 MTR_2g066980 36.034 358 168 13 7 343 1 318 1.09e-67 215
MS.gene59493.t1 MTR_7g078630 44.948 287 116 6 28 303 695 950 1.43e-67 228
MS.gene59493.t1 MTR_4g065077 39.130 322 177 9 32 343 44 356 2.15e-67 216
MS.gene59493.t1 MTR_2g015930 39.241 316 176 10 37 343 50 358 3.15e-64 207
MS.gene59493.t1 MTR_4g071060 39.252 321 166 11 37 340 45 353 6.78e-64 207
MS.gene59493.t1 MTR_1g023210 36.982 338 170 12 23 335 33 352 8.16e-62 201
MS.gene59493.t1 MTR_1g023810 41.481 270 132 8 75 341 15 261 7.80e-61 196
MS.gene59493.t1 MTR_8g086470 36.875 320 171 9 32 343 60 356 8.79e-59 194
MS.gene59493.t1 MTR_1g094135 35.014 357 197 10 7 340 19 363 2.40e-58 193
MS.gene59493.t1 MTR_4g072790 33.025 324 169 11 25 340 34 317 4.15e-43 152
MS.gene59493.t1 MTR_2g015840 50.993 151 68 4 16 161 7 156 8.04e-43 149
MS.gene59493.t1 MTR_2g015840 57.143 112 47 1 232 343 161 271 1.78e-36 133
MS.gene59493.t1 MTR_4g072340 32.416 327 171 11 25 340 34 321 6.53e-41 146
MS.gene59493.t1 MTR_4g073660 32.416 327 171 11 25 340 33 320 8.04e-41 146
MS.gene59493.t1 MTR_7g078580 63.265 98 35 1 245 342 4 100 2.58e-40 138
MS.gene59493.t1 MTR_7g079130 68.889 90 28 0 253 342 7 96 1.36e-39 135
MS.gene59493.t1 MTR_1g025450 39.344 183 84 8 120 296 47 208 4.54e-31 123
MS.gene59493.t1 MTR_7g078310 44.286 140 69 2 82 219 115 247 1.31e-28 112
MS.gene59493.t1 MTR_2g016380 46.957 115 56 3 232 343 23 135 2.67e-26 105
MS.gene59493.t1 MTR_7g111060 27.309 249 149 7 112 335 89 330 4.56e-23 98.6
MS.gene59493.t1 MTR_7g079260 59.722 72 13 1 269 340 7 62 3.17e-22 90.5
MS.gene59493.t1 MTR_7g111050 29.362 235 136 7 132 343 110 337 6.65e-22 95.5
MS.gene59493.t1 MTR_3g029560 26.000 250 153 7 98 343 16 237 7.62e-21 90.5
MS.gene59493.t1 MTR_8g087510 41.221 131 70 5 167 295 23 148 1.09e-19 85.5
MS.gene59493.t1 MTR_2g047960 48.235 85 43 1 135 219 7 90 5.51e-18 78.6
MS.gene59493.t1 MTR_3g082370 25.980 204 131 5 131 319 5 203 1.71e-16 78.2
MS.gene59493.t1 MTR_7g111050 27.179 195 118 5 132 305 105 296 2.60e-12 67.0
MS.gene59493.t1 MTR_1g042480 34.043 94 59 2 9 101 7 98 5.33e-11 60.1
MS.gene59493.t1 MTR_1g042360 34.043 94 59 2 9 101 7 98 5.33e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59493.t1 AT5G45890 54.203 345 148 4 7 343 3 345 2.47e-134 386
MS.gene59493.t1 AT5G50260 53.913 345 135 6 14 342 5 341 1.23e-128 372
MS.gene59493.t1 AT3G48340 53.890 347 136 8 12 342 4 342 1.89e-123 359
MS.gene59493.t1 AT2G34080 50.000 342 160 6 10 344 8 345 6.28e-120 350
MS.gene59493.t1 AT3G49340 50.585 342 144 7 20 344 8 341 5.97e-118 345
MS.gene59493.t1 AT3G19390 50.453 331 160 4 14 343 18 345 1.32e-117 348
MS.gene59493.t1 AT4G35350 53.035 313 136 5 35 343 47 352 4.85e-115 338
MS.gene59493.t1 AT2G27420 48.276 348 159 6 14 344 5 348 1.16e-114 336
MS.gene59493.t1 AT3G48350 55.484 310 129 7 39 343 38 343 2.84e-114 336
MS.gene59493.t1 AT5G43060 53.067 326 139 7 24 342 34 352 3.42e-114 339
MS.gene59493.t1 AT1G29090 47.458 354 168 8 4 344 7 355 1.61e-112 331
MS.gene59493.t1 AT3G19400 50.814 307 147 4 39 343 44 348 4.32e-111 328
MS.gene59493.t1 AT1G06260 50.294 340 156 7 9 342 9 341 4.01e-110 325
MS.gene59493.t1 AT4G36880 50.462 325 143 6 32 343 42 361 7.96e-110 325
MS.gene59493.t1 AT1G20850 51.923 312 138 5 37 343 49 353 8.71e-110 324
MS.gene59493.t1 AT1G47128 51.133 309 139 7 39 342 50 351 2.39e-109 327
MS.gene59493.t1 AT1G29080 47.337 338 166 7 15 344 13 346 1.19e-106 316
MS.gene59493.t1 AT1G09850 49.351 308 153 3 35 342 28 332 5.94e-101 305
MS.gene59493.t1 AT4G23520 49.515 309 142 9 40 342 48 348 7.96e-101 301
MS.gene59493.t1 AT4G11310 47.249 309 150 6 40 342 50 351 4.13e-98 295
MS.gene59493.t1 AT4G11320 46.278 309 153 6 40 342 57 358 5.49e-97 292
MS.gene59493.t1 AT4G11320 46.278 309 153 6 40 342 57 358 5.49e-97 292
MS.gene59493.t1 AT3G43960 44.377 329 176 6 18 342 20 345 9.87e-89 271
MS.gene59493.t1 AT1G29110 43.168 322 160 7 29 343 28 333 7.62e-88 268
MS.gene59493.t1 AT4G35350 50.202 247 112 5 35 277 47 286 7.00e-81 248
MS.gene59493.t1 AT3G19400 48.963 241 120 3 39 277 44 283 1.49e-77 239
MS.gene59493.t1 AT3G48350 52.869 244 106 7 39 277 38 277 1.01e-75 235
MS.gene59493.t1 AT5G60360 43.182 308 158 10 41 343 61 356 1.01e-71 227
MS.gene59493.t1 AT5G60360 42.857 308 158 11 41 343 61 355 3.61e-69 220
MS.gene59493.t1 AT3G45310 41.100 309 165 10 41 344 61 357 1.32e-67 216
MS.gene59493.t1 AT5G60360 42.475 299 155 10 41 334 61 347 2.59e-67 216
MS.gene59493.t1 AT3G45310 40.777 309 165 11 41 344 61 356 5.55e-65 209
MS.gene59493.t1 AT4G39090 38.854 314 159 11 46 340 58 357 8.46e-63 204
MS.gene59493.t1 AT2G21430 38.111 307 161 10 46 335 55 349 2.43e-62 203
MS.gene59493.t1 AT4G16190 39.365 315 162 10 44 340 60 363 9.66e-60 196
MS.gene59493.t1 AT3G54940 37.658 316 160 10 42 335 54 354 2.42e-57 190
MS.gene59493.t1 AT3G54940 37.991 229 125 5 42 259 54 276 8.84e-43 150
MS.gene59493.t1 AT1G02305 26.446 363 198 15 6 335 7 333 8.87e-24 100
MS.gene59493.t1 AT4G01610 26.224 286 179 8 75 335 52 330 4.03e-21 93.2
MS.gene59493.t1 AT4G01610 25.874 286 180 8 75 335 52 330 5.57e-20 90.1
MS.gene59493.t1 AT1G02300 28.910 211 124 5 145 335 146 350 3.44e-19 88.2
MS.gene59493.t1 AT2G22160 42.718 103 54 3 43 145 1 98 4.18e-17 76.3

Find 85 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCAATTGCTACAGAAAT+TGG 0.238620 5.4:-14074473 None:intergenic
AATATGATATAATTGATTAT+TGG 0.245014 5.4:-14073612 None:intergenic
TACAAAAGTGGTGTATTTAC+TGG 0.253916 5.4:+14074518 MS.gene59493:CDS
TACATTGAAGCTTTCAATAA+TGG 0.255324 5.4:+14073809 MS.gene59493:CDS
TGTTCCATCAACACCTTGAT+AGG 0.273901 5.4:-14074348 None:intergenic
TTCCTCCAATCCACTGTAGA+TGG 0.300644 5.4:-14073983 None:intergenic
ATCTATGCAGCTACTATTAC+CGG 0.345117 5.4:+14074392 MS.gene59493:CDS
TTGCAATGCTTGCTCATTGT+TGG 0.345681 5.4:-14074432 None:intergenic
TGATTAATGCCTAGTTTGTA+TGG 0.354887 5.4:-14073845 None:intergenic
GAACTCATGGGGAACTGATT+GGG 0.362958 5.4:+14074625 MS.gene59493:CDS
AAATTCATCATTCAAAATCA+TGG 0.364764 5.4:+14074302 MS.gene59493:CDS
GCAACCTTGGTCCACACCTT+TGG 0.378000 5.4:-14074252 None:intergenic
TACCCCTATCAAGGTGTTGA+TGG 0.400072 5.4:+14074344 MS.gene59493:CDS
TCAATTATATCATATTTCAT+TGG 0.405472 5.4:+14073619 MS.gene59493:CDS
GGTGTCACTGCCGTAGGTTA+TGG 0.419357 5.4:+14074566 MS.gene59493:CDS
AGGTTGCGAGGGTGGTCTAA+TGG 0.427677 5.4:+14074268 MS.gene59493:CDS
GGTAATAGTAGCTGCATAGA+TGG 0.434640 5.4:-14074390 None:intergenic
GCTCTGTGGCATTGCAATGC+AGG 0.434661 5.4:+14074697 MS.gene59493:CDS
AGAACTCATGGGGAACTGAT+TGG 0.436487 5.4:+14074624 MS.gene59493:CDS
AATGCTTGCTCATTGTTGGC+AGG 0.438570 5.4:-14074428 None:intergenic
TAAGTTGAGTACTGAAAAGT+TGG 0.443110 5.4:+14074193 MS.gene59493:CDS
CATTGGCCTTGGTTCTTCAC+AGG 0.447016 5.4:-14074022 None:intergenic
GACACTTACCACATTGGCCT+TGG 0.449261 5.4:-14074033 None:intergenic
ATATCATATTTCATTGGCAT+TGG 0.451673 5.4:+14073625 MS.gene59493:CDS
AGGATACATTATGATGCAAA+GGG 0.457248 5.4:+14074655 MS.gene59493:CDS
GGTTATGGTGTTAGTAATGA+TGG 0.466813 5.4:+14074581 MS.gene59493:CDS
GTTCTTAACCAACCAATACT+TGG 0.469107 5.4:-14074606 None:intergenic
GGAACTGATTGGGGTGAAGA+AGG 0.474210 5.4:+14074635 MS.gene59493:CDS
AAGTTTGGCATTTAAGCAGT+AGG 0.477282 5.4:-14074728 None:intergenic
TCTCATGCATGGAACCATCT+TGG 0.477831 5.4:-14073694 None:intergenic
AGATGCTACAAACTCCTCAT+TGG 0.495942 5.4:-14073882 None:intergenic
ATTGGTTGGTTAAGAACTCA+TGG 0.497126 5.4:+14074612 MS.gene59493:CDS
GAGCAGTGGATGAGTCACTA+TGG 0.500053 5.4:+14073722 MS.gene59493:CDS
CAATTCCATCTACAGTGGAT+TGG 0.509500 5.4:+14073978 MS.gene59493:CDS
TCTGCTGTTGCAGCAACTGA+AGG 0.516542 5.4:+14074164 MS.gene59493:CDS
GTAATGATGGAACCAAGTAT+TGG 0.517960 5.4:+14074594 MS.gene59493:CDS
GTGGACCAAGGTTGCGAGGG+TGG 0.519583 5.4:+14074260 MS.gene59493:CDS
AGCATCTAGGAACAAATTCA+AGG 0.524056 5.4:+14073898 MS.gene59493:CDS
CTGTAGCAATTGATGCTAGT+GGG 0.531888 5.4:+14074480 MS.gene59493:CDS
CTGCTCATGCCTCTCATGCA+TGG 0.532697 5.4:-14073705 None:intergenic
TTCCATCTACAGTGGATTGG+AGG 0.533019 5.4:+14073981 MS.gene59493:CDS
TGGTTCATGCGGAACTGAGT+TGG 0.533466 5.4:+14074538 MS.gene59493:CDS
ACAGTGGATTGGAGGAAGAA+AGG 0.534477 5.4:+14073989 MS.gene59493:CDS
AAGGATACATTATGATGCAA+AGG 0.535444 5.4:+14074654 MS.gene59493:CDS
GTTCCATCAACACCTTGATA+GGG 0.537305 5.4:-14074347 None:intergenic
TAATTGATTATTGGCAGCCA+TGG 0.544682 5.4:-14073603 None:intergenic
TTAGACCACCCTCGCAACCT+TGG 0.545380 5.4:-14074265 None:intergenic
AGGGGAGTTGAAGCTGCTGA+AGG 0.548793 5.4:+14074674 MS.gene59493:CDS
TCTGTAGCAATTGATGCTAG+TGG 0.551551 5.4:+14074479 MS.gene59493:CDS
TAGATAGACACTTACCACAT+TGG 0.554580 5.4:-14074039 None:intergenic
AGTTTGGCATTTAAGCAGTA+GGG 0.554948 5.4:-14074727 None:intergenic
ATGAGGAGTTTGTAGCATCT+AGG 0.557730 5.4:+14073885 MS.gene59493:CDS
GAAGCTCAATACCCCTATCA+AGG 0.559311 5.4:+14074335 MS.gene59493:CDS
AACTCATGGGGAACTGATTG+GGG 0.563189 5.4:+14074626 MS.gene59493:CDS
TGATGGAACCAAGTATTGGT+TGG 0.568906 5.4:+14074598 MS.gene59493:CDS
TTGGTTGGTTAAGAACTCAT+GGG 0.574582 5.4:+14074613 MS.gene59493:CDS
ACTTCTCGCACTCTCCAAGA+TGG 0.576062 5.4:+14073680 MS.gene59493:CDS
ATGCTTGCTCATTGTTGGCA+GGG 0.585035 5.4:-14074427 None:intergenic
AGAAATTGGTTGATTTGCCA+CGG 0.587106 5.4:-14074459 None:intergenic
AGTAACAAACCATACAAACT+AGG 0.588321 5.4:+14073836 MS.gene59493:CDS
TGCGGAACTGAGTTGGATCA+CGG 0.591647 5.4:+14074545 MS.gene59493:CDS
AGGTGTGGACCAAGGTTGCG+AGG 0.594152 5.4:+14074256 MS.gene59493:CDS
GGGGAGTTGAAGCTGCTGAA+GGG 0.599415 5.4:+14074675 MS.gene59493:CDS
TTCCATCAACACCTTGATAG+GGG 0.602425 5.4:-14074346 None:intergenic
CTTGTTGATTGCGACACCAA+AGG 0.605991 5.4:+14074236 MS.gene59493:CDS
GGCAGGGACATCTTCATACC+CGG 0.606676 5.4:-14074411 None:intergenic
GCATCTAGGAACAAATTCAA+GGG 0.615159 5.4:+14073899 MS.gene59493:CDS
TGCATGAGAGGCATGAGCAG+TGG 0.619804 5.4:+14073708 MS.gene59493:CDS
ATTACTAACACCATAACCTA+CGG 0.625557 5.4:-14074576 None:intergenic
GGTGTGGACCAAGGTTGCGA+GGG 0.629368 5.4:+14074257 MS.gene59493:CDS
GATCACGGTGTCACTGCCGT+AGG 0.641748 5.4:+14074560 MS.gene59493:CDS
GGTGTATTTACTGGTTCATG+CGG 0.645001 5.4:+14074527 MS.gene59493:CDS
TGGTTGGTTAAGAACTCATG+GGG 0.647156 5.4:+14074614 MS.gene59493:CDS
TCTATGCAGCTACTATTACC+GGG 0.649142 5.4:+14074393 MS.gene59493:CDS
AAGATGGTTCCATGCATGAG+AGG 0.652611 5.4:+14073696 MS.gene59493:CDS
CTACAAACTCCTCATTGGTG+AGG 0.661600 5.4:-14073877 None:intergenic
ATTTGCAGACCTCACCAATG+AGG 0.664553 5.4:+14073868 MS.gene59493:CDS
GTGAAGAACCAAGGCCAATG+TGG 0.667923 5.4:+14074025 MS.gene59493:CDS
AAGTGCAATTCCATCTACAG+TGG 0.669087 5.4:+14073973 MS.gene59493:CDS
GACACCAAAGGTGTGGACCA+AGG 0.670622 5.4:+14074248 MS.gene59493:CDS
TGATTGCGACACCAAAGGTG+TGG 0.678453 5.4:+14074241 MS.gene59493:CDS
ATATGTGCTCTTCAATCACA+AGG 0.681864 5.4:+14073924 MS.gene59493:CDS
GAAGCTGCTGAAGGGCTCTG+TGG 0.682304 5.4:+14074683 MS.gene59493:CDS
GTGACACCTGTGAAGAACCA+AGG 0.693484 5.4:+14074016 MS.gene59493:CDS
GGATACATTATGATGCAAAG+GGG 0.728766 5.4:+14074656 MS.gene59493:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGATATAATTGATTAT+TGG - chr5.4:14073615-14073634 None:intergenic 10.0%
!! TCAATTATATCATATTTCAT+TGG + chr5.4:14073619-14073638 MS.gene59493:CDS 15.0%
!! AAATTCATCATTCAAAATCA+TGG + chr5.4:14074302-14074321 MS.gene59493:CDS 20.0%
!!! AATTGAATTTTCATCTAACT+CGG + chr5.4:14074099-14074118 MS.gene59493:intron 20.0%
!!! AATTTTAACTTGCATTTTGT+AGG + chr5.4:14074125-14074144 MS.gene59493:intron 20.0%
! ATATCATATTTCATTGGCAT+TGG + chr5.4:14073625-14073644 MS.gene59493:CDS 25.0%
! TACATTGAAGCTTTCAATAA+TGG + chr5.4:14073809-14073828 MS.gene59493:CDS 25.0%
!! ATTGAATTTTCATCTAACTC+GGG + chr5.4:14074100-14074119 MS.gene59493:intron 25.0%
!! GACTTTCAATTTTACAAAAG+TGG + chr5.4:14074506-14074525 MS.gene59493:CDS 25.0%
AAGGATACATTATGATGCAA+AGG + chr5.4:14074654-14074673 MS.gene59493:CDS 30.0%
AGGATACATTATGATGCAAA+GGG + chr5.4:14074655-14074674 MS.gene59493:CDS 30.0%
AGTAACAAACCATACAAACT+AGG + chr5.4:14073836-14073855 MS.gene59493:CDS 30.0%
ATTACTAACACCATAACCTA+CGG - chr5.4:14074579-14074598 None:intergenic 30.0%
TACAAAAGTGGTGTATTTAC+TGG + chr5.4:14074518-14074537 MS.gene59493:CDS 30.0%
! TGATTAATGCCTAGTTTGTA+TGG - chr5.4:14073848-14073867 None:intergenic 30.0%
!! TAAGTTGAGTACTGAAAAGT+TGG + chr5.4:14074193-14074212 MS.gene59493:CDS 30.0%
AGCATCTAGGAACAAATTCA+AGG + chr5.4:14073898-14073917 MS.gene59493:CDS 35.0%
ATATGTGCTCTTCAATCACA+AGG + chr5.4:14073924-14073943 MS.gene59493:CDS 35.0%
ATCTATGCAGCTACTATTAC+CGG + chr5.4:14074392-14074411 MS.gene59493:CDS 35.0%
GCATCAATTGCTACAGAAAT+TGG - chr5.4:14074476-14074495 None:intergenic 35.0%
GCATCTAGGAACAAATTCAA+GGG + chr5.4:14073899-14073918 MS.gene59493:CDS 35.0%
GGATACATTATGATGCAAAG+GGG + chr5.4:14074656-14074675 MS.gene59493:CDS 35.0%
GTTCTTAACCAACCAATACT+TGG - chr5.4:14074609-14074628 None:intergenic 35.0%
TAGATAGACACTTACCACAT+TGG - chr5.4:14074042-14074061 None:intergenic 35.0%
! ATTGGTTGGTTAAGAACTCA+TGG + chr5.4:14074612-14074631 MS.gene59493:CDS 35.0%
! GCATTTTGTAGGATGTTGTT+GGG + chr5.4:14074136-14074155 MS.gene59493:intron 35.0%
! GGTTATGGTGTTAGTAATGA+TGG + chr5.4:14074581-14074600 MS.gene59493:CDS 35.0%
! GTAATGATGGAACCAAGTAT+TGG + chr5.4:14074594-14074613 MS.gene59493:CDS 35.0%
! TGCATTTTGTAGGATGTTGT+TGG + chr5.4:14074135-14074154 MS.gene59493:intron 35.0%
! TTGGTTGGTTAAGAACTCAT+GGG + chr5.4:14074613-14074632 MS.gene59493:CDS 35.0%
!! AGAAATTGGTTGATTTGCCA+CGG - chr5.4:14074462-14074481 None:intergenic 35.0%
AAGTGCAATTCCATCTACAG+TGG + chr5.4:14073973-14073992 MS.gene59493:CDS 40.0%
AGATGCTACAAACTCCTCAT+TGG - chr5.4:14073885-14073904 None:intergenic 40.0%
ATGAGGAGTTTGTAGCATCT+AGG + chr5.4:14073885-14073904 MS.gene59493:CDS 40.0%
CAATTCCATCTACAGTGGAT+TGG + chr5.4:14073978-14073997 MS.gene59493:CDS 40.0%
CTGTAGCAATTGATGCTAGT+GGG + chr5.4:14074480-14074499 MS.gene59493:CDS 40.0%
GGTAATAGTAGCTGCATAGA+TGG - chr5.4:14074393-14074412 None:intergenic 40.0%
GTTCCATCAACACCTTGATA+GGG - chr5.4:14074350-14074369 None:intergenic 40.0%
TCTATGCAGCTACTATTACC+GGG + chr5.4:14074393-14074412 MS.gene59493:CDS 40.0%
TCTGTAGCAATTGATGCTAG+TGG + chr5.4:14074479-14074498 MS.gene59493:CDS 40.0%
TGTTCCATCAACACCTTGAT+AGG - chr5.4:14074351-14074370 None:intergenic 40.0%
TTCCATCAACACCTTGATAG+GGG - chr5.4:14074349-14074368 None:intergenic 40.0%
TTGCAATGCTTGCTCATTGT+TGG - chr5.4:14074435-14074454 None:intergenic 40.0%
! TGGTTGGTTAAGAACTCATG+GGG + chr5.4:14074614-14074633 MS.gene59493:CDS 40.0%
!! GGTGTATTTACTGGTTCATG+CGG + chr5.4:14074527-14074546 MS.gene59493:CDS 40.0%
!! TGATGGAACCAAGTATTGGT+TGG + chr5.4:14074598-14074617 MS.gene59493:CDS 40.0%
!!! ATTGGCATTGGTTTTCTGCT+TGG + chr5.4:14073637-14073656 MS.gene59493:CDS 40.0%
!!! TTGGCATTGGTTTTCTGCTT+GGG + chr5.4:14073638-14073657 MS.gene59493:CDS 40.0%
AACTCATGGGGAACTGATTG+GGG + chr5.4:14074626-14074645 MS.gene59493:CDS 45.0%
AAGATGGTTCCATGCATGAG+AGG + chr5.4:14073696-14073715 MS.gene59493:CDS 45.0%
AATGCTTGCTCATTGTTGGC+AGG - chr5.4:14074431-14074450 None:intergenic 45.0%
AGAACTCATGGGGAACTGAT+TGG + chr5.4:14074624-14074643 MS.gene59493:CDS 45.0%
ATGCTTGCTCATTGTTGGCA+GGG - chr5.4:14074430-14074449 None:intergenic 45.0%
ATTTGCAGACCTCACCAATG+AGG + chr5.4:14073868-14073887 MS.gene59493:CDS 45.0%
CTACAAACTCCTCATTGGTG+AGG - chr5.4:14073880-14073899 None:intergenic 45.0%
GAACTCATGGGGAACTGATT+GGG + chr5.4:14074625-14074644 MS.gene59493:CDS 45.0%
GAAGCTCAATACCCCTATCA+AGG + chr5.4:14074335-14074354 MS.gene59493:CDS 45.0%
TCTCATGCATGGAACCATCT+TGG - chr5.4:14073697-14073716 None:intergenic 45.0%
TTCCTCCAATCCACTGTAGA+TGG - chr5.4:14073986-14074005 None:intergenic 45.0%
! CTTGTTGATTGCGACACCAA+AGG + chr5.4:14074236-14074255 MS.gene59493:CDS 45.0%
! GGTTTTCTGCTTGGGATTGT+GGG + chr5.4:14073646-14073665 MS.gene59493:CDS 45.0%
! TGGTTTTCTGCTTGGGATTG+TGG + chr5.4:14073645-14073664 MS.gene59493:CDS 45.0%
!! ACAGTGGATTGGAGGAAGAA+AGG + chr5.4:14073989-14074008 MS.gene59493:CDS 45.0%
!! TACCCCTATCAAGGTGTTGA+TGG + chr5.4:14074344-14074363 MS.gene59493:CDS 45.0%
!! TTCCATCTACAGTGGATTGG+AGG + chr5.4:14073981-14074000 MS.gene59493:CDS 45.0%
ACTTCTCGCACTCTCCAAGA+TGG + chr5.4:14073680-14073699 MS.gene59493:CDS 50.0%
GAGCAGTGGATGAGTCACTA+TGG + chr5.4:14073722-14073741 MS.gene59493:CDS 50.0%
GGAACTGATTGGGGTGAAGA+AGG + chr5.4:14074635-14074654 MS.gene59493:CDS 50.0%
GTGAAGAACCAAGGCCAATG+TGG + chr5.4:14074025-14074044 MS.gene59493:CDS 50.0%
GTGACACCTGTGAAGAACCA+AGG + chr5.4:14074016-14074035 MS.gene59493:CDS 50.0%
TCTGCTGTTGCAGCAACTGA+AGG + chr5.4:14074164-14074183 MS.gene59493:CDS 50.0%
TGATTGCGACACCAAAGGTG+TGG + chr5.4:14074241-14074260 MS.gene59493:CDS 50.0%
TGGTTCATGCGGAACTGAGT+TGG + chr5.4:14074538-14074557 MS.gene59493:CDS 50.0%
! GACACTTACCACATTGGCCT+TGG - chr5.4:14074036-14074055 None:intergenic 50.0%
! GCAAGCATTGCAAAAAGCCG+TGG + chr5.4:14074442-14074461 MS.gene59493:CDS 50.0%
! TGCGGAACTGAGTTGGATCA+CGG + chr5.4:14074545-14074564 MS.gene59493:CDS 50.0%
!! CATTGGCCTTGGTTCTTCAC+AGG - chr5.4:14074025-14074044 None:intergenic 50.0%
AGGGGAGTTGAAGCTGCTGA+AGG + chr5.4:14074674-14074693 MS.gene59493:CDS 55.0%
AGGTTGCGAGGGTGGTCTAA+TGG + chr5.4:14074268-14074287 MS.gene59493:CDS 55.0%
CTGCTCATGCCTCTCATGCA+TGG - chr5.4:14073708-14073727 None:intergenic 55.0%
GCAACCTTGGTCCACACCTT+TGG - chr5.4:14074255-14074274 None:intergenic 55.0%
GCTCTGTGGCATTGCAATGC+AGG + chr5.4:14074697-14074716 MS.gene59493:CDS 55.0%
GGCAGGGACATCTTCATACC+CGG - chr5.4:14074414-14074433 None:intergenic 55.0%
GGGGAGTTGAAGCTGCTGAA+GGG + chr5.4:14074675-14074694 MS.gene59493:CDS 55.0%
TTAGACCACCCTCGCAACCT+TGG - chr5.4:14074268-14074287 None:intergenic 55.0%
! GACACCAAAGGTGTGGACCA+AGG + chr5.4:14074248-14074267 MS.gene59493:CDS 55.0%
! TGCATGAGAGGCATGAGCAG+TGG + chr5.4:14073708-14073727 MS.gene59493:CDS 55.0%
!! GGTGTCACTGCCGTAGGTTA+TGG + chr5.4:14074566-14074585 MS.gene59493:CDS 55.0%
AGGTGTGGACCAAGGTTGCG+AGG + chr5.4:14074256-14074275 MS.gene59493:CDS 60.0%
GAAGCTGCTGAAGGGCTCTG+TGG + chr5.4:14074683-14074702 MS.gene59493:CDS 60.0%
GGTGTGGACCAAGGTTGCGA+GGG + chr5.4:14074257-14074276 MS.gene59493:CDS 60.0%
!! GATCACGGTGTCACTGCCGT+AGG + chr5.4:14074560-14074579 MS.gene59493:CDS 60.0%
GTGGACCAAGGTTGCGAGGG+TGG + chr5.4:14074260-14074279 MS.gene59493:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 14073605 14074739 14073605 ID=MS.gene59493
chr5.4 mRNA 14073605 14074739 14073605 ID=MS.gene59493.t1;Parent=MS.gene59493
chr5.4 exon 14073605 14074046 14073605 ID=MS.gene59493.t1.exon1;Parent=MS.gene59493.t1
chr5.4 CDS 14073605 14074046 14073605 ID=cds.MS.gene59493.t1;Parent=MS.gene59493.t1
chr5.4 exon 14074147 14074739 14074147 ID=MS.gene59493.t1.exon2;Parent=MS.gene59493.t1
chr5.4 CDS 14074147 14074739 14074147 ID=cds.MS.gene59493.t1;Parent=MS.gene59493.t1
Gene Sequence

>MS.gene59493

ATGGCTGCCAATAATCAATTATATCATATTTCATTGGCATTGGTTTTCTGCTTGGGATTGTGGGCTATTCAAGTCACTTCTCGCACTCTCCAAGATGGTTCCATGCATGAGAGGCATGAGCAGTGGATGAGTCACTATGGCAAAGTCTATAATGATCATCAAGAACGAGAGAAGCGTTTCAAGATATTCACAGAAAACATGAAATACATTGAAGCTTTCAATAATGGTGACAGTAACAAACCATACAAACTAGGCATTAATCAATTTGCAGACCTCACCAATGAGGAGTTTGTAGCATCTAGGAACAAATTCAAGGGACATATGTGCTCTTCAATCACAAGGACAACTACTTTTAAGTATGAAAATGTAAGTGCAATTCCATCTACAGTGGATTGGAGGAAGAAAGGAGCAGTGACACCTGTGAAGAACCAAGGCCAATGTGGTAAGTGTCTATCTAAAAACTAATGCATTTAATTCAATATAATGTGATCAAAAATTGAATTTTCATCTAACTCGGGTAAATTTTAACTTGCATTTTGTAGGATGTTGTTGGGCGTTTTCTGCTGTTGCAGCAACTGAAGGAATTCATAAGTTGAGTACTGAAAAGTTGGTCTCTTTATCAGAACAAGAACTTGTTGATTGCGACACCAAAGGTGTGGACCAAGGTTGCGAGGGTGGTCTAATGGATGATGCTTTCAAATTCATCATTCAAAATCATGGACTCAGCACTGAAGCTCAATACCCCTATCAAGGTGTTGATGGAACATGCAATGCAAACAAAGCATCCATCTATGCAGCTACTATTACCGGGTATGAAGATGTCCCTGCCAACAATGAGCAAGCATTGCAAAAAGCCGTGGCAAATCAACCAATTTCTGTAGCAATTGATGCTAGTGGGTCTGACTTTCAATTTTACAAAAGTGGTGTATTTACTGGTTCATGCGGAACTGAGTTGGATCACGGTGTCACTGCCGTAGGTTATGGTGTTAGTAATGATGGAACCAAGTATTGGTTGGTTAAGAACTCATGGGGAACTGATTGGGGTGAAGAAGGATACATTATGATGCAAAGGGGAGTTGAAGCTGCTGAAGGGCTCTGTGGCATTGCAATGCAGGCATCTTACCCTACTGCTTAA
Protein sequence

>MS.gene59493.t1

MAANNQLYHISLALVFCLGLWAIQVTSRTLQDGSMHERHEQWMSHYGKVYNDHQEREKRFKIFTENMKYIEAFNNGDSNKPYKLGINQFADLTNEEFVASRNKFKGHMCSSITRTTTFKYENVSAIPSTVDWRKKGAVTPVKNQGQCGCCWAFSAVAATEGIHKLSTEKLVSLSEQELVDCDTKGVDQGCEGGLMDDAFKFIIQNHGLSTEAQYPYQGVDGTCNANKASIYAATITGYEDVPANNEQALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGVSNDGTKYWLVKNSWGTDWGEEGYIMMQRGVEAAEGLCGIAMQASYPTA