Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59648.t1 | XP_003614457.2 | 98.7 | 308 | 4 | 0 | 1 | 308 | 1 | 308 | 8.20E-175 | 622.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59648.t1 | Q9FV52 | 71.3 | 314 | 80 | 2 | 5 | 308 | 8 | 321 | 4.8e-123 | 442.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59648.t1 | G7JY96 | 98.7 | 308 | 4 | 0 | 1 | 308 | 1 | 308 | 5.9e-175 | 622.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049284 | MS.gene59648 | 0.818949 | 1.50E-52 | -1.69E-46 |
| MS.gene049297 | MS.gene59648 | 0.81761 | 3.02E-52 | -1.69E-46 |
| MS.gene049351 | MS.gene59648 | 0.81574 | 7.92E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene59648 | 0.808936 | 2.42E-50 | -1.69E-46 |
| MS.gene050076 | MS.gene59648 | 0.838777 | 2.35E-57 | -1.69E-46 |
| MS.gene050099 | MS.gene59648 | 0.808747 | 2.66E-50 | -1.69E-46 |
| MS.gene050301 | MS.gene59648 | 0.864895 | 8.74E-65 | -1.69E-46 |
| MS.gene050397 | MS.gene59648 | 0.80714 | 5.84E-50 | -1.69E-46 |
| MS.gene050404 | MS.gene59648 | 0.831226 | 1.88E-55 | -1.69E-46 |
| MS.gene050406 | MS.gene59648 | 0.835254 | 1.87E-56 | -1.69E-46 |
| MS.gene050464 | MS.gene59648 | 0.827835 | 1.26E-54 | -1.69E-46 |
| MS.gene050529 | MS.gene59648 | 0.835531 | 1.59E-56 | -1.69E-46 |
| MS.gene050642 | MS.gene59648 | 0.816994 | 4.15E-52 | -1.69E-46 |
| MS.gene050783 | MS.gene59648 | 0.832412 | 9.60E-56 | -1.69E-46 |
| MS.gene050812 | MS.gene59648 | 0.839908 | 1.20E-57 | -1.69E-46 |
| MS.gene05101 | MS.gene59648 | 0.855963 | 4.43E-62 | -1.69E-46 |
| MS.gene051171 | MS.gene59648 | 0.833561 | 4.97E-56 | -1.69E-46 |
| MS.gene051240 | MS.gene59648 | 0.822553 | 2.23E-53 | -1.69E-46 |
| MS.gene051242 | MS.gene59648 | 0.839523 | 1.51E-57 | -1.69E-46 |
| MS.gene051471 | MS.gene59648 | 0.861705 | 8.50E-64 | -1.69E-46 |
| MS.gene051582 | MS.gene59648 | 0.850064 | 2.16E-60 | -1.69E-46 |
| MS.gene051662 | MS.gene59648 | 0.816667 | 4.92E-52 | -1.69E-46 |
| MS.gene051983 | MS.gene59648 | 0.80027 | 1.56E-48 | -1.69E-46 |
| MS.gene052078 | MS.gene59648 | 0.829157 | 6.02E-55 | -1.69E-46 |
| MS.gene052178 | MS.gene59648 | 0.813464 | 2.53E-51 | -1.69E-46 |
| MS.gene052310 | MS.gene59648 | 0.828291 | 9.75E-55 | -1.69E-46 |
| MS.gene052326 | MS.gene59648 | 0.826149 | 3.18E-54 | -1.69E-46 |
| MS.gene053330 | MS.gene59648 | 0.831531 | 1.58E-55 | -1.69E-46 |
| MS.gene053557 | MS.gene59648 | 0.813428 | 2.57E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene59648 | 0.852192 | 5.42E-61 | -1.69E-46 |
| MS.gene053907 | MS.gene59648 | 0.932322 | 1.01E-94 | -1.69E-46 |
| MS.gene053971 | MS.gene59648 | 0.900103 | 1.02E-77 | -1.69E-46 |
| MS.gene054123 | MS.gene59648 | 0.814844 | 1.25E-51 | -1.69E-46 |
| MS.gene05455 | MS.gene59648 | 0.804117 | 2.52E-49 | -1.69E-46 |
| MS.gene054942 | MS.gene59648 | 0.824025 | 1.01E-53 | -1.69E-46 |
| MS.gene054983 | MS.gene59648 | 0.809642 | 1.71E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene59648 | 0.839607 | 1.43E-57 | -1.69E-46 |
| MS.gene055390 | MS.gene59648 | 0.82326 | 1.52E-53 | -1.69E-46 |
| MS.gene055422 | MS.gene59648 | 0.845956 | 2.94E-59 | -1.69E-46 |
| MS.gene055428 | MS.gene59648 | 0.849312 | 3.51E-60 | -1.69E-46 |
| MS.gene055533 | MS.gene59648 | 0.803266 | 3.78E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene59648 | 0.823922 | 1.07E-53 | -1.69E-46 |
| MS.gene055980 | MS.gene59648 | 0.80429 | 2.32E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene59648 | 0.807559 | 4.76E-50 | -1.69E-46 |
| MS.gene056451 | MS.gene59648 | 0.854138 | 1.50E-61 | -1.69E-46 |
| MS.gene056612 | MS.gene59648 | 0.880207 | 6.64E-70 | -1.69E-46 |
| MS.gene056633 | MS.gene59648 | 0.826984 | 2.01E-54 | -1.69E-46 |
| MS.gene056664 | MS.gene59648 | 0.848558 | 5.68E-60 | -1.69E-46 |
| MS.gene056772 | MS.gene59648 | 0.806132 | 9.54E-50 | -1.69E-46 |
| MS.gene056899 | MS.gene59648 | -0.811181 | 7.96E-51 | -1.69E-46 |
| MS.gene057011 | MS.gene59648 | 0.808816 | 2.57E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene59648 | 0.81603 | 6.82E-52 | -1.69E-46 |
| MS.gene057121 | MS.gene59648 | 0.850881 | 1.27E-60 | -1.69E-46 |
| MS.gene057245 | MS.gene59648 | 0.827713 | 1.34E-54 | -1.69E-46 |
| MS.gene057439 | MS.gene59648 | 0.829604 | 4.69E-55 | -1.69E-46 |
| MS.gene058002 | MS.gene59648 | 0.847315 | 1.25E-59 | -1.69E-46 |
| MS.gene05811 | MS.gene59648 | 0.832672 | 8.27E-56 | -1.69E-46 |
| MS.gene058142 | MS.gene59648 | 0.861626 | 8.99E-64 | -1.69E-46 |
| MS.gene058233 | MS.gene59648 | 0.885067 | 1.12E-71 | -1.69E-46 |
| MS.gene058276 | MS.gene59648 | 0.875237 | 3.61E-68 | -1.69E-46 |
| MS.gene058293 | MS.gene59648 | 0.82393 | 1.06E-53 | -1.69E-46 |
| MS.gene058365 | MS.gene59648 | 0.801015 | 1.10E-48 | -1.69E-46 |
| MS.gene058660 | MS.gene59648 | 0.84265 | 2.27E-58 | -1.69E-46 |
| MS.gene058746 | MS.gene59648 | 0.846602 | 1.96E-59 | -1.69E-46 |
| MS.gene058845 | MS.gene59648 | 0.803437 | 3.49E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene59648 | 0.835149 | 1.98E-56 | -1.69E-46 |
| MS.gene059518 | MS.gene59648 | 0.822749 | 2.01E-53 | -1.69E-46 |
| MS.gene059597 | MS.gene59648 | 0.828919 | 6.88E-55 | -1.69E-46 |
| MS.gene05959 | MS.gene59648 | 0.804559 | 2.04E-49 | -1.69E-46 |
| MS.gene059676 | MS.gene59648 | 0.811727 | 6.06E-51 | -1.69E-46 |
| MS.gene05984 | MS.gene59648 | 0.815329 | 9.78E-52 | -1.69E-46 |
| MS.gene059894 | MS.gene59648 | 0.866382 | 2.97E-65 | -1.69E-46 |
| MS.gene059899 | MS.gene59648 | 0.804234 | 2.38E-49 | -1.69E-46 |
| MS.gene060407 | MS.gene59648 | 0.874558 | 6.15E-68 | -1.69E-46 |
| MS.gene06090 | MS.gene59648 | 0.848215 | 7.07E-60 | -1.69E-46 |
| MS.gene061049 | MS.gene59648 | 0.85113 | 1.08E-60 | -1.69E-46 |
| MS.gene061112 | MS.gene59648 | 0.8028 | 4.72E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59648.t1 | MTR_5g053940 | 98.701 | 308 | 4 | 0 | 1 | 308 | 1 | 308 | 0.0 | 635 |
| MS.gene59648.t1 | MTR_5g053940 | 99.270 | 274 | 2 | 0 | 35 | 308 | 11 | 284 | 0.0 | 571 |
| MS.gene59648.t1 | MTR_5g017630 | 63.846 | 260 | 89 | 1 | 49 | 308 | 49 | 303 | 1.82e-116 | 338 |
| MS.gene59648.t1 | MTR_5g017630 | 63.359 | 262 | 91 | 1 | 47 | 308 | 47 | 303 | 2.99e-116 | 339 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59648.t1 | AT1G13270 | 73.422 | 301 | 77 | 2 | 9 | 308 | 23 | 321 | 7.82e-157 | 443 |
| MS.gene59648.t1 | AT3G25740 | 72.047 | 254 | 68 | 2 | 57 | 308 | 43 | 295 | 7.18e-128 | 368 |
| MS.gene59648.t1 | AT1G13270 | 71.429 | 252 | 69 | 2 | 9 | 259 | 23 | 272 | 3.70e-124 | 357 |
| MS.gene59648.t1 | AT4G37040 | 57.095 | 296 | 123 | 3 | 14 | 308 | 10 | 302 | 3.18e-112 | 329 |
| MS.gene59648.t1 | AT2G45240 | 47.479 | 238 | 119 | 3 | 77 | 308 | 100 | 337 | 1.07e-69 | 222 |
| MS.gene59648.t1 | AT2G45240 | 46.606 | 221 | 113 | 2 | 77 | 292 | 100 | 320 | 3.37e-65 | 208 |
| MS.gene59648.t1 | AT3G25740 | 69.444 | 144 | 44 | 0 | 59 | 202 | 45 | 188 | 7.35e-63 | 199 |
Find 68 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATATTCAGTTCCCATTTC+TGG | 0.169592 | 5.4:-42618663 | None:intergenic |
| TGGATACCTCTTGATTCTTC+TGG | 0.183050 | 5.4:-42616550 | None:intergenic |
| TATCCCTCACCCCTTGGTTA+TGG | 0.287436 | 5.4:+42621181 | MS.gene59648:CDS |
| AGGTGATGTGTCGCCATAAA+AGG | 0.304209 | 5.4:-42616317 | None:intergenic |
| TCAATTTCTACCACGTTTCC+CGG | 0.315068 | 5.4:+42616346 | MS.gene59648:CDS |
| ACCTGATTCACGGCAGTTAC+AGG | 0.325575 | 5.4:+42621258 | MS.gene59648:CDS |
| ACCTGTAACTGCCGTGAATC+AGG | 0.336343 | 5.4:-42621259 | None:intergenic |
| TCACGATGATGGTAAATATA+TGG | 0.340395 | 5.4:-42625372 | None:intergenic |
| CTCAGATGTACTTCCAGAAA+TGG | 0.356951 | 5.4:+42618650 | MS.gene59648:CDS |
| ATGAAGAGAACGTGAACTTC+AGG | 0.387349 | 5.4:+42618530 | MS.gene59648:intron |
| CCCTCACCCCTTGGTTATGG+TGG | 0.394047 | 5.4:+42621184 | MS.gene59648:CDS |
| TCCTTTCTCCAAGCACTCTT+CGG | 0.408446 | 5.4:-42622517 | None:intergenic |
| ATAGAAAAGGGCCTCCATTA+AGG | 0.411437 | 5.4:+42618565 | MS.gene59648:CDS |
| ACTTCGCAGGAACTTTGGTT+AGG | 0.412046 | 5.4:+42618751 | MS.gene59648:CDS |
| TCGCTTTGTTAGGGATATCA+TGG | 0.416903 | 5.4:+42622213 | MS.gene59648:intron |
| GAAGGTCAAACATTTACAAT+TGG | 0.419869 | 5.4:+42625785 | MS.gene59648:CDS |
| AGTGAGCATGCCGAAAAGTA+TGG | 0.420168 | 5.4:+42625291 | MS.gene59648:intron |
| GGAATAGCCGTTTGCAAAGA+TGG | 0.430491 | 5.4:+42622537 | MS.gene59648:CDS |
| CTTGAACTTCGCAGGAACTT+TGG | 0.435030 | 5.4:+42618746 | MS.gene59648:CDS |
| GGTGCTTATCCCTCACCCCT+TGG | 0.435960 | 5.4:+42621175 | MS.gene59648:CDS |
| GGAGGCTTTAGTATATCATC+AGG | 0.458379 | 5.4:-42618618 | None:intergenic |
| CTTCGCAGGAACTTTGGTTA+GGG | 0.460318 | 5.4:+42618752 | MS.gene59648:CDS |
| TGCTCGGAAGATGTCAGGAT+TGG | 0.472168 | 5.4:+42616521 | MS.gene59648:CDS |
| CACCAGCATCAATTATCATC+TGG | 0.488043 | 5.4:-42621156 | None:intergenic |
| GCCATGGGATACCTGATTCA+CGG | 0.494701 | 5.4:+42621248 | MS.gene59648:CDS |
| TCGGAAGATGTCAGGATTGG+AGG | 0.503158 | 5.4:+42616524 | MS.gene59648:CDS |
| TGAACTTCAGGTTGTGAAGA+AGG | 0.505374 | 5.4:+42618542 | MS.gene59648:CDS |
| TCAGATGTACTTCCAGAAAT+GGG | 0.509891 | 5.4:+42618651 | MS.gene59648:CDS |
| ATACTAAAGCCTCCTTATGT+AGG | 0.510983 | 5.4:+42618627 | MS.gene59648:CDS |
| CTGAAGGTATAGCTAAAATG+AGG | 0.514873 | 5.4:+42618697 | MS.gene59648:CDS |
| ATAGGTAACCGAAGAGTGCT+TGG | 0.515902 | 5.4:+42622509 | MS.gene59648:intron |
| ATAGCTAAAATGAGGGCTGC+AGG | 0.530647 | 5.4:+42618705 | MS.gene59648:CDS |
| GAAGGAGGTTGCATGGTTGA+AGG | 0.534832 | 5.4:+42625767 | MS.gene59648:CDS |
| GTGGTAGAAATTGAAGAAGA+AGG | 0.536961 | 5.4:-42616337 | None:intergenic |
| GCACGTGTCTTGAACTTCGC+AGG | 0.540825 | 5.4:+42618738 | MS.gene59648:CDS |
| ACCGAAGAGTGCTTGGAGAA+AGG | 0.543493 | 5.4:+42622516 | MS.gene59648:CDS |
| AGTACATCTGAGCCTACATA+AGG | 0.545561 | 5.4:-42618639 | None:intergenic |
| AGGGGTACCATCTTTGCAAA+CGG | 0.547784 | 5.4:-42622544 | None:intergenic |
| ATTTCATTAGTTGTTACAGA+AGG | 0.554257 | 5.4:-42621118 | None:intergenic |
| TGAAAGGTAACTCACGATGA+TGG | 0.557241 | 5.4:-42625383 | None:intergenic |
| CCACCATAACCAAGGGGTGA+GGG | 0.564039 | 5.4:-42621184 | None:intergenic |
| TATCAAATTCATGATTCTGA+AGG | 0.565467 | 5.4:+42618681 | MS.gene59648:CDS |
| CGGAAATCCACCATAACCAA+GGG | 0.568072 | 5.4:-42621191 | None:intergenic |
| TCCACCATAACCAAGGGGTG+AGG | 0.570980 | 5.4:-42621185 | None:intergenic |
| AAAGTATGGCTACGGTGTAG+TGG | 0.575901 | 5.4:+42625305 | MS.gene59648:CDS |
| ATGGCGCTCACTGCTTCACT+CGG | 0.577075 | 5.4:+42616265 | MS.gene59648:CDS |
| TTTAGTATATCATCAGGGAC+AGG | 0.579400 | 5.4:-42618612 | None:intergenic |
| GTTGTGAAGAAGGATAGAAA+AGG | 0.584695 | 5.4:+42618552 | MS.gene59648:CDS |
| TTGTGAAGAAGGATAGAAAA+GGG | 0.586969 | 5.4:+42618553 | MS.gene59648:CDS |
| GTTAATGAGTGCATGTGCCA+TGG | 0.601267 | 5.4:+42621232 | MS.gene59648:CDS |
| CATGCCGAAAAGTATGGCTA+CGG | 0.602255 | 5.4:+42625297 | MS.gene59648:CDS |
| AATTAACAAACCGGGAAACG+TGG | 0.607568 | 5.4:-42616356 | None:intergenic |
| CAATGACGAAGGAGGTTGCA+TGG | 0.610246 | 5.4:+42625760 | MS.gene59648:CDS |
| CACCAGATGATAATTGATGC+TGG | 0.614026 | 5.4:+42621154 | MS.gene59648:CDS |
| GAAGATGTCAGGATTGGAGG+AGG | 0.614618 | 5.4:+42616527 | MS.gene59648:CDS |
| GCCGTGAATCAGGTATCCCA+TGG | 0.621584 | 5.4:-42621249 | None:intergenic |
| TTAATGAGTGCATGTGCCAT+GGG | 0.622408 | 5.4:+42621233 | MS.gene59648:CDS |
| TCGGAAATCCACCATAACCA+AGG | 0.629493 | 5.4:-42621192 | None:intergenic |
| AGTAGTGAGTTTCAGAAATG+CGG | 0.629669 | 5.4:-42616293 | None:intergenic |
| TTGATAGGCAATGACGAAGG+AGG | 0.640673 | 5.4:+42625752 | MS.gene59648:intron |
| ACATCTGAGCCTACATAAGG+AGG | 0.644417 | 5.4:-42618636 | None:intergenic |
| AAAGGGCCTCCATTAAGGCG+TGG | 0.649969 | 5.4:+42618570 | MS.gene59648:CDS |
| GAGGCTTTAGTATATCATCA+GGG | 0.660911 | 5.4:-42618617 | None:intergenic |
| TGAAGGTATAGCTAAAATGA+GGG | 0.663053 | 5.4:+42618698 | MS.gene59648:CDS |
| AGGAGGCCAGAAGAATCAAG+AGG | 0.691066 | 5.4:+42616544 | MS.gene59648:CDS |
| TGCTTGATAGGCAATGACGA+AGG | 0.699722 | 5.4:+42625749 | MS.gene59648:intron |
| TCATCAGGGACAGGTAGACG+TGG | 0.713408 | 5.4:-42618603 | None:intergenic |
| GGAAATCCACCATAACCAAG+GGG | 0.749657 | 5.4:-42621190 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 42616265 | 42625806 | 42616265 | ID=MS.gene59648 |
| chr5.4 | mRNA | 42616265 | 42625806 | 42616265 | ID=MS.gene59648.t1;Parent=MS.gene59648 |
| chr5.4 | exon | 42616265 | 42616367 | 42616265 | ID=MS.gene59648.t1.exon1;Parent=MS.gene59648.t1 |
| chr5.4 | CDS | 42616265 | 42616367 | 42616265 | ID=cds.MS.gene59648.t1;Parent=MS.gene59648.t1 |
| chr5.4 | exon | 42616484 | 42616565 | 42616484 | ID=MS.gene59648.t1.exon2;Parent=MS.gene59648.t1 |
| chr5.4 | CDS | 42616484 | 42616565 | 42616484 | ID=cds.MS.gene59648.t1;Parent=MS.gene59648.t1 |
| chr5.4 | exon | 42618536 | 42618773 | 42618536 | ID=MS.gene59648.t1.exon3;Parent=MS.gene59648.t1 |
| chr5.4 | CDS | 42618536 | 42618773 | 42618536 | ID=cds.MS.gene59648.t1;Parent=MS.gene59648.t1 |
| chr5.4 | exon | 42621118 | 42621279 | 42621118 | ID=MS.gene59648.t1.exon4;Parent=MS.gene59648.t1 |
| chr5.4 | CDS | 42621118 | 42621279 | 42621118 | ID=cds.MS.gene59648.t1;Parent=MS.gene59648.t1 |
| chr5.4 | exon | 42621367 | 42621408 | 42621367 | ID=MS.gene59648.t1.exon5;Parent=MS.gene59648.t1 |
| chr5.4 | CDS | 42621367 | 42621408 | 42621367 | ID=cds.MS.gene59648.t1;Parent=MS.gene59648.t1 |
| chr5.4 | exon | 42622225 | 42622296 | 42622225 | ID=MS.gene59648.t1.exon6;Parent=MS.gene59648.t1 |
| chr5.4 | CDS | 42622225 | 42622296 | 42622225 | ID=cds.MS.gene59648.t1;Parent=MS.gene59648.t1 |
| chr5.4 | exon | 42622513 | 42622592 | 42622513 | ID=MS.gene59648.t1.exon7;Parent=MS.gene59648.t1 |
| chr5.4 | CDS | 42622513 | 42622592 | 42622513 | ID=cds.MS.gene59648.t1;Parent=MS.gene59648.t1 |
| chr5.4 | exon | 42625293 | 42625390 | 42625293 | ID=MS.gene59648.t1.exon8;Parent=MS.gene59648.t1 |
| chr5.4 | CDS | 42625293 | 42625390 | 42625293 | ID=cds.MS.gene59648.t1;Parent=MS.gene59648.t1 |
| chr5.4 | exon | 42625759 | 42625806 | 42625759 | ID=MS.gene59648.t1.exon9;Parent=MS.gene59648.t1 |
| chr5.4 | CDS | 42625759 | 42625806 | 42625759 | ID=cds.MS.gene59648.t1;Parent=MS.gene59648.t1 |
| Gene Sequence |
| Protein sequence |
