Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59906.t1 | KEH27958.1 | 99.3 | 289 | 2 | 0 | 1 | 289 | 44 | 332 | 2.80E-169 | 604.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59906.t1 | Q9FV52 | 81.1 | 286 | 54 | 0 | 4 | 289 | 84 | 369 | 1.9e-137 | 490.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59906.t1 | G7JY96 | 99.3 | 289 | 2 | 0 | 1 | 289 | 68 | 356 | 2.0e-169 | 604.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049284 | MS.gene59906 | 0.819135 | 1.36E-52 | -1.69E-46 |
| MS.gene049297 | MS.gene59906 | 0.832454 | 9.37E-56 | -1.69E-46 |
| MS.gene049758 | MS.gene59906 | 0.827712 | 1.34E-54 | -1.69E-46 |
| MS.gene049910 | MS.gene59906 | 0.808368 | 3.21E-50 | -1.69E-46 |
| MS.gene050076 | MS.gene59906 | 0.82866 | 7.95E-55 | -1.69E-46 |
| MS.gene050099 | MS.gene59906 | 0.808455 | 3.07E-50 | -1.69E-46 |
| MS.gene050301 | MS.gene59906 | 0.863837 | 1.87E-64 | -1.69E-46 |
| MS.gene050330 | MS.gene59906 | -0.805261 | 1.45E-49 | -1.69E-46 |
| MS.gene050404 | MS.gene59906 | 0.839351 | 1.67E-57 | -1.69E-46 |
| MS.gene050406 | MS.gene59906 | 0.843665 | 1.22E-58 | -1.69E-46 |
| MS.gene050464 | MS.gene59906 | 0.832543 | 8.90E-56 | -1.69E-46 |
| MS.gene050529 | MS.gene59906 | 0.828322 | 9.59E-55 | -1.69E-46 |
| MS.gene050558 | MS.gene59906 | 0.806839 | 6.77E-50 | -1.69E-46 |
| MS.gene050642 | MS.gene59906 | 0.841007 | 6.17E-58 | -1.69E-46 |
| MS.gene050783 | MS.gene59906 | 0.804479 | 2.12E-49 | -1.69E-46 |
| MS.gene050812 | MS.gene59906 | 0.806866 | 6.68E-50 | -1.69E-46 |
| MS.gene05101 | MS.gene59906 | 0.846244 | 2.45E-59 | -1.69E-46 |
| MS.gene051171 | MS.gene59906 | 0.833381 | 5.51E-56 | -1.69E-46 |
| MS.gene051240 | MS.gene59906 | 0.842022 | 3.33E-58 | -1.69E-46 |
| MS.gene051242 | MS.gene59906 | 0.836858 | 7.31E-57 | -1.69E-46 |
| MS.gene051471 | MS.gene59906 | 0.857014 | 2.18E-62 | -1.69E-46 |
| MS.gene051582 | MS.gene59906 | 0.849265 | 3.61E-60 | -1.69E-46 |
| MS.gene051662 | MS.gene59906 | 0.83848 | 2.81E-57 | -1.69E-46 |
| MS.gene051663 | MS.gene59906 | 0.80306 | 4.17E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene59906 | 0.825459 | 4.64E-54 | -1.69E-46 |
| MS.gene052078 | MS.gene59906 | 0.830625 | 2.64E-55 | -1.69E-46 |
| MS.gene052178 | MS.gene59906 | 0.830348 | 3.09E-55 | -1.69E-46 |
| MS.gene052309 | MS.gene59906 | -0.814624 | 1.40E-51 | -1.69E-46 |
| MS.gene052310 | MS.gene59906 | 0.829596 | 4.71E-55 | -1.69E-46 |
| MS.gene052326 | MS.gene59906 | 0.811196 | 7.90E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene59906 | 0.808111 | 3.64E-50 | -1.69E-46 |
| MS.gene052600 | MS.gene59906 | 0.813391 | 2.62E-51 | -1.69E-46 |
| MS.gene053330 | MS.gene59906 | 0.82109 | 4.86E-53 | -1.69E-46 |
| MS.gene053557 | MS.gene59906 | 0.822569 | 2.21E-53 | -1.69E-46 |
| MS.gene053836 | MS.gene59906 | 0.864384 | 1.26E-64 | -1.69E-46 |
| MS.gene053907 | MS.gene59906 | 0.923363 | 2.93E-89 | -1.69E-46 |
| MS.gene053971 | MS.gene59906 | 0.895684 | 7.59E-76 | -1.69E-46 |
| MS.gene054123 | MS.gene59906 | 0.813718 | 2.22E-51 | -1.69E-46 |
| MS.gene054473 | MS.gene59906 | 0.804356 | 2.25E-49 | -1.69E-46 |
| MS.gene05455 | MS.gene59906 | 0.805004 | 1.65E-49 | -1.69E-46 |
| MS.gene054942 | MS.gene59906 | 0.84144 | 4.75E-58 | -1.69E-46 |
| MS.gene054983 | MS.gene59906 | 0.8361 | 1.14E-56 | -1.69E-46 |
| MS.gene055277 | MS.gene59906 | 0.837126 | 6.25E-57 | -1.69E-46 |
| MS.gene055390 | MS.gene59906 | 0.816229 | 6.16E-52 | -1.69E-46 |
| MS.gene055422 | MS.gene59906 | 0.852227 | 5.30E-61 | -1.69E-46 |
| MS.gene055428 | MS.gene59906 | 0.8566 | 2.88E-62 | -1.69E-46 |
| MS.gene05553 | MS.gene59906 | 0.832278 | 1.04E-55 | -1.69E-46 |
| MS.gene055980 | MS.gene59906 | 0.812213 | 4.75E-51 | -1.69E-46 |
| MS.gene056451 | MS.gene59906 | 0.844999 | 5.34E-59 | -1.69E-46 |
| MS.gene056612 | MS.gene59906 | 0.881355 | 2.57E-70 | -1.69E-46 |
| MS.gene056633 | MS.gene59906 | 0.819403 | 1.18E-52 | -1.69E-46 |
| MS.gene056664 | MS.gene59906 | 0.834787 | 2.45E-56 | -1.69E-46 |
| MS.gene056772 | MS.gene59906 | 0.824399 | 8.24E-54 | -1.69E-46 |
| MS.gene056899 | MS.gene59906 | -0.809654 | 1.70E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene59906 | 0.819898 | 9.12E-53 | -1.69E-46 |
| MS.gene05702 | MS.gene59906 | 0.821336 | 4.26E-53 | -1.69E-46 |
| MS.gene057121 | MS.gene59906 | 0.847997 | 8.11E-60 | -1.69E-46 |
| MS.gene057245 | MS.gene59906 | 0.839188 | 1.84E-57 | -1.69E-46 |
| MS.gene057439 | MS.gene59906 | 0.858016 | 1.10E-62 | -1.69E-46 |
| MS.gene058002 | MS.gene59906 | 0.843823 | 1.11E-58 | -1.69E-46 |
| MS.gene05811 | MS.gene59906 | 0.842502 | 2.49E-58 | -1.69E-46 |
| MS.gene058142 | MS.gene59906 | 0.867012 | 1.87E-65 | -1.69E-46 |
| MS.gene058233 | MS.gene59906 | 0.874621 | 5.85E-68 | -1.69E-46 |
| MS.gene058276 | MS.gene59906 | 0.873777 | 1.13E-67 | -1.69E-46 |
| MS.gene058293 | MS.gene59906 | 0.817518 | 3.16E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene59906 | 0.820649 | 6.14E-53 | -1.69E-46 |
| MS.gene058746 | MS.gene59906 | 0.842855 | 2.00E-58 | -1.69E-46 |
| MS.gene058845 | MS.gene59906 | 0.826602 | 2.48E-54 | -1.69E-46 |
| MS.gene05907 | MS.gene59906 | 0.833291 | 5.80E-56 | -1.69E-46 |
| MS.gene059518 | MS.gene59906 | 0.827512 | 1.50E-54 | -1.69E-46 |
| MS.gene059597 | MS.gene59906 | 0.828047 | 1.12E-54 | -1.69E-46 |
| MS.gene059676 | MS.gene59906 | 0.802438 | 5.61E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene59906 | 0.833729 | 4.51E-56 | -1.69E-46 |
| MS.gene059894 | MS.gene59906 | 0.885341 | 8.84E-72 | -1.69E-46 |
| MS.gene059899 | MS.gene59906 | 0.827263 | 1.72E-54 | -1.69E-46 |
| MS.gene060407 | MS.gene59906 | 0.862941 | 3.55E-64 | -1.69E-46 |
| MS.gene06090 | MS.gene59906 | 0.817139 | 3.85E-52 | -1.69E-46 |
| MS.gene061049 | MS.gene59906 | 0.865076 | 7.68E-65 | -1.69E-46 |
| MS.gene061112 | MS.gene59906 | 0.802278 | 6.05E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59906.t1 | MTR_5g053940 | 99.308 | 289 | 2 | 0 | 1 | 289 | 68 | 356 | 0.0 | 603 |
| MS.gene59906.t1 | MTR_5g053940 | 99.308 | 289 | 2 | 0 | 1 | 289 | 44 | 332 | 0.0 | 601 |
| MS.gene59906.t1 | MTR_5g017630 | 68.817 | 279 | 87 | 0 | 11 | 289 | 73 | 351 | 9.12e-142 | 403 |
| MS.gene59906.t1 | MTR_5g017630 | 67.932 | 237 | 76 | 0 | 11 | 247 | 73 | 309 | 8.46e-116 | 336 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59906.t1 | AT1G13270 | 81.119 | 286 | 54 | 0 | 4 | 289 | 84 | 369 | 2.75e-175 | 489 |
| MS.gene59906.t1 | AT3G25740 | 74.126 | 286 | 71 | 2 | 4 | 287 | 57 | 341 | 1.46e-152 | 430 |
| MS.gene59906.t1 | AT4G37040 | 65.614 | 285 | 98 | 0 | 5 | 289 | 66 | 350 | 6.70e-135 | 385 |
| MS.gene59906.t1 | AT1G13270 | 81.481 | 189 | 35 | 0 | 4 | 192 | 84 | 272 | 1.59e-110 | 321 |
| MS.gene59906.t1 | AT2G45240 | 48.944 | 284 | 139 | 3 | 10 | 287 | 100 | 383 | 5.62e-91 | 276 |
| MS.gene59906.t1 | AT2G45240 | 46.606 | 221 | 113 | 2 | 10 | 225 | 100 | 320 | 1.12e-64 | 206 |
| MS.gene59906.t1 | AT3G25740 | 71.212 | 132 | 38 | 0 | 4 | 135 | 57 | 188 | 3.71e-58 | 187 |
Find 67 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATATTCAGTTCCCATTTC+TGG | 0.169592 | 5.4:-42432868 | None:intergenic |
| GTAACTGTTGTCCAGTTATC+TGG | 0.270072 | 5.4:-42440451 | None:intergenic |
| TATCCCTCACCCCTTGGTTA+TGG | 0.287436 | 5.4:+42435386 | MS.gene59906:CDS |
| ACCTGATTCACGGCAGTTAC+AGG | 0.325575 | 5.4:+42435463 | MS.gene59906:CDS |
| ACCTGTAACTGCCGTGAATC+AGG | 0.336343 | 5.4:-42435464 | None:intergenic |
| TCACGATGATGGTAAATATA+TGG | 0.340395 | 5.4:-42439575 | None:intergenic |
| TCCAGTTCTAGTTATCAAAA+TGG | 0.343628 | 5.4:-42440509 | None:intergenic |
| GCATTACATGGCCAGATAAC+TGG | 0.349858 | 5.4:+42440440 | MS.gene59906:CDS |
| CTCAGATGTACTTCCAGAAA+TGG | 0.356951 | 5.4:+42432855 | MS.gene59906:CDS |
| CCCTCACCCCTTGGTTATGG+TGG | 0.394047 | 5.4:+42435389 | MS.gene59906:CDS |
| TGCAGAGCCTATTCTTTCAA+TGG | 0.401624 | 5.4:+42440405 | MS.gene59906:intron |
| TCCTTTCTCCAAGCACTCTT+CGG | 0.408446 | 5.4:-42436722 | None:intergenic |
| ATAGAAAAGGGCCTCCATTA+AGG | 0.411437 | 5.4:+42432770 | MS.gene59906:CDS |
| ACTTCGCAGGAACTTTGGTT+AGG | 0.412046 | 5.4:+42432956 | MS.gene59906:CDS |
| TCGCTTTGTTAGGGATATCA+TGG | 0.416903 | 5.4:+42436418 | MS.gene59906:intron |
| GAAGGTCAAACATTTACAAT+TGG | 0.419869 | 5.4:+42439988 | MS.gene59906:CDS |
| AGTGAGCATGCCGAAAAGTA+TGG | 0.420168 | 5.4:+42439494 | MS.gene59906:intron |
| GGAATAGCCGTTTGCAAAGA+TGG | 0.430491 | 5.4:+42436742 | MS.gene59906:CDS |
| CTTGAACTTCGCAGGAACTT+TGG | 0.435030 | 5.4:+42432951 | MS.gene59906:CDS |
| GGTGCTTATCCCTCACCCCT+TGG | 0.435960 | 5.4:+42435380 | MS.gene59906:CDS |
| GCAGAGCCTATTCTTTCAAT+GGG | 0.447951 | 5.4:+42440406 | MS.gene59906:intron |
| GGAGGCTTTAGTATATCATC+AGG | 0.458379 | 5.4:-42432823 | None:intergenic |
| CTTCGCAGGAACTTTGGTTA+GGG | 0.460318 | 5.4:+42432957 | MS.gene59906:CDS |
| GTGCTTCCCATTGAAAGAAT+AGG | 0.481596 | 5.4:-42440412 | None:intergenic |
| CACCAGCATCAATTATCATC+TGG | 0.488043 | 5.4:-42435361 | None:intergenic |
| GCCATGGGATACCTGATTCA+CGG | 0.494701 | 5.4:+42435453 | MS.gene59906:CDS |
| TGAACTTCAGGTTGTGAAGA+AGG | 0.505374 | 5.4:+42432747 | None:intergenic |
| TCAGATGTACTTCCAGAAAT+GGG | 0.509891 | 5.4:+42432856 | MS.gene59906:CDS |
| ATACTAAAGCCTCCTTATGT+AGG | 0.510983 | 5.4:+42432832 | MS.gene59906:CDS |
| CTGAAGGTATAGCTAAAATG+AGG | 0.514873 | 5.4:+42432902 | MS.gene59906:CDS |
| ATAGGTAACCGAAGAGTGCT+TGG | 0.515902 | 5.4:+42436714 | MS.gene59906:intron |
| ATAGCTAAAATGAGGGCTGC+AGG | 0.530647 | 5.4:+42432910 | MS.gene59906:CDS |
| GTATGCTCAAACTGTGCGGC+CGG | 0.531387 | 5.4:-42440487 | None:intergenic |
| GAAGGAGGTTGCATGGTTGA+AGG | 0.534832 | 5.4:+42439970 | MS.gene59906:CDS |
| GCACGTGTCTTGAACTTCGC+AGG | 0.540825 | 5.4:+42432943 | MS.gene59906:CDS |
| AGTTACAACTGATGGTAGTC+CGG | 0.540857 | 5.4:+42440468 | MS.gene59906:CDS |
| ACCGAAGAGTGCTTGGAGAA+AGG | 0.543493 | 5.4:+42436721 | MS.gene59906:CDS |
| AGTACATCTGAGCCTACATA+AGG | 0.545561 | 5.4:-42432844 | None:intergenic |
| AGGGGTACCATCTTTGCAAA+CGG | 0.547784 | 5.4:-42436749 | None:intergenic |
| ATTTCATTAGTTGTTACAGA+AGG | 0.554257 | 5.4:-42435323 | None:intergenic |
| TGAAAGGTAACTCACGATGA+TGG | 0.557241 | 5.4:-42439586 | None:intergenic |
| CCACCATAACCAAGGGGTGA+GGG | 0.564039 | 5.4:-42435389 | None:intergenic |
| TATCAAATTCATGATTCTGA+AGG | 0.565467 | 5.4:+42432886 | MS.gene59906:CDS |
| CGGAAATCCACCATAACCAA+GGG | 0.568072 | 5.4:-42435396 | None:intergenic |
| TCCACCATAACCAAGGGGTG+AGG | 0.570980 | 5.4:-42435390 | None:intergenic |
| AAAGTATGGCTACGGTGTAG+TGG | 0.575901 | 5.4:+42439508 | MS.gene59906:CDS |
| TGGACAACAGTTACAACTGA+TGG | 0.577735 | 5.4:+42440460 | MS.gene59906:CDS |
| TTTAGTATATCATCAGGGAC+AGG | 0.579400 | 5.4:-42432817 | None:intergenic |
| GTTGTGAAGAAGGATAGAAA+AGG | 0.584695 | 5.4:+42432757 | MS.gene59906:CDS |
| TTGTGAAGAAGGATAGAAAA+GGG | 0.586969 | 5.4:+42432758 | MS.gene59906:CDS |
| GTTAATGAGTGCATGTGCCA+TGG | 0.601267 | 5.4:+42435437 | MS.gene59906:CDS |
| CATGCCGAAAAGTATGGCTA+CGG | 0.602255 | 5.4:+42439500 | MS.gene59906:CDS |
| CAATGACGAAGGAGGTTGCA+TGG | 0.610246 | 5.4:+42439963 | MS.gene59906:CDS |
| CACCAGATGATAATTGATGC+TGG | 0.614026 | 5.4:+42435359 | MS.gene59906:CDS |
| GCCGTGAATCAGGTATCCCA+TGG | 0.621584 | 5.4:-42435454 | None:intergenic |
| TTAATGAGTGCATGTGCCAT+GGG | 0.622408 | 5.4:+42435438 | MS.gene59906:CDS |
| GAAGCACTGATTGCATTACA+TGG | 0.627144 | 5.4:+42440428 | MS.gene59906:CDS |
| TCGGAAATCCACCATAACCA+AGG | 0.629493 | 5.4:-42435397 | None:intergenic |
| TTGATAGGCAATGACGAAGG+AGG | 0.640673 | 5.4:+42439955 | MS.gene59906:intron |
| ACATCTGAGCCTACATAAGG+AGG | 0.644417 | 5.4:-42432841 | None:intergenic |
| AAAGGGCCTCCATTAAGGCG+TGG | 0.649969 | 5.4:+42432775 | MS.gene59906:CDS |
| GAGGCTTTAGTATATCATCA+GGG | 0.660911 | 5.4:-42432822 | None:intergenic |
| TGAAGGTATAGCTAAAATGA+GGG | 0.663053 | 5.4:+42432903 | MS.gene59906:CDS |
| TGCTTGATAGGCAATGACGA+AGG | 0.699722 | 5.4:+42439952 | MS.gene59906:intron |
| AATGGTATGCTCAAACTGTG+CGG | 0.700072 | 5.4:-42440491 | None:intergenic |
| TCATCAGGGACAGGTAGACG+TGG | 0.713408 | 5.4:-42432808 | None:intergenic |
| GGAAATCCACCATAACCAAG+GGG | 0.749657 | 5.4:-42435395 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 42432757 | 42440555 | 42432757 | ID=MS.gene59906 |
| chr5.4 | mRNA | 42432757 | 42440555 | 42432757 | ID=MS.gene59906.t1;Parent=MS.gene59906 |
| chr5.4 | exon | 42432757 | 42432978 | 42432757 | ID=MS.gene59906.t1.exon1;Parent=MS.gene59906.t1 |
| chr5.4 | CDS | 42432757 | 42432978 | 42432757 | ID=cds.MS.gene59906.t1;Parent=MS.gene59906.t1 |
| chr5.4 | exon | 42435323 | 42435484 | 42435323 | ID=MS.gene59906.t1.exon2;Parent=MS.gene59906.t1 |
| chr5.4 | CDS | 42435323 | 42435484 | 42435323 | ID=cds.MS.gene59906.t1;Parent=MS.gene59906.t1 |
| chr5.4 | exon | 42435572 | 42435613 | 42435572 | ID=MS.gene59906.t1.exon3;Parent=MS.gene59906.t1 |
| chr5.4 | CDS | 42435572 | 42435613 | 42435572 | ID=cds.MS.gene59906.t1;Parent=MS.gene59906.t1 |
| chr5.4 | exon | 42436430 | 42436501 | 42436430 | ID=MS.gene59906.t1.exon4;Parent=MS.gene59906.t1 |
| chr5.4 | CDS | 42436430 | 42436501 | 42436430 | ID=cds.MS.gene59906.t1;Parent=MS.gene59906.t1 |
| chr5.4 | exon | 42436718 | 42436797 | 42436718 | ID=MS.gene59906.t1.exon5;Parent=MS.gene59906.t1 |
| chr5.4 | CDS | 42436718 | 42436797 | 42436718 | ID=cds.MS.gene59906.t1;Parent=MS.gene59906.t1 |
| chr5.4 | exon | 42439496 | 42439593 | 42439496 | ID=MS.gene59906.t1.exon6;Parent=MS.gene59906.t1 |
| chr5.4 | CDS | 42439496 | 42439593 | 42439496 | ID=cds.MS.gene59906.t1;Parent=MS.gene59906.t1 |
| chr5.4 | exon | 42439962 | 42440009 | 42439962 | ID=MS.gene59906.t1.exon7;Parent=MS.gene59906.t1 |
| chr5.4 | CDS | 42439962 | 42440009 | 42439962 | ID=cds.MS.gene59906.t1;Parent=MS.gene59906.t1 |
| chr5.4 | exon | 42440410 | 42440555 | 42440410 | ID=MS.gene59906.t1.exon8;Parent=MS.gene59906.t1 |
| chr5.4 | CDS | 42440410 | 42440555 | 42440410 | ID=cds.MS.gene59906.t1;Parent=MS.gene59906.t1 |
| Gene Sequence |
| Protein sequence |
