Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62501.t1 | XP_013464200.1 | 99.5 | 215 | 1 | 0 | 1 | 215 | 1 | 215 | 2.80E-121 | 444.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62501.t1 | Q8BZJ7 | 40.9 | 220 | 122 | 3 | 1 | 213 | 1 | 219 | 3.3e-46 | 186.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62501.t1 | A0A072VJB0 | 99.5 | 215 | 1 | 0 | 1 | 215 | 1 | 215 | 2.0e-121 | 444.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene62501 | 0.820349 | 7.19E-53 | -1.69E-46 |
| MS.gene049166 | MS.gene62501 | 0.80001 | 1.76E-48 | -1.69E-46 |
| MS.gene049822 | MS.gene62501 | 0.800791 | 1.22E-48 | -1.69E-46 |
| MS.gene050084 | MS.gene62501 | 0.838907 | 2.18E-57 | -1.69E-46 |
| MS.gene050085 | MS.gene62501 | 0.854001 | 1.65E-61 | -1.69E-46 |
| MS.gene050114 | MS.gene62501 | 0.850442 | 1.69E-60 | -1.69E-46 |
| MS.gene050116 | MS.gene62501 | 0.822009 | 2.98E-53 | -1.69E-46 |
| MS.gene050317 | MS.gene62501 | 0.824607 | 7.37E-54 | -1.69E-46 |
| MS.gene050359 | MS.gene62501 | 0.83044 | 2.93E-55 | -1.69E-46 |
| MS.gene050439 | MS.gene62501 | 0.801685 | 8.01E-49 | -1.69E-46 |
| MS.gene050556 | MS.gene62501 | 0.807626 | 4.61E-50 | -1.69E-46 |
| MS.gene050654 | MS.gene62501 | 0.827464 | 1.54E-54 | -1.69E-46 |
| MS.gene051102 | MS.gene62501 | 0.811648 | 6.30E-51 | -1.69E-46 |
| MS.gene051184 | MS.gene62501 | 0.812772 | 3.58E-51 | -1.69E-46 |
| MS.gene051343 | MS.gene62501 | 0.841486 | 4.62E-58 | -1.69E-46 |
| MS.gene051381 | MS.gene62501 | 0.814308 | 1.65E-51 | -1.69E-46 |
| MS.gene051676 | MS.gene62501 | 0.813692 | 2.25E-51 | -1.69E-46 |
| MS.gene051757 | MS.gene62501 | 0.800747 | 1.25E-48 | -1.69E-46 |
| MS.gene051831 | MS.gene62501 | 0.81543 | 9.28E-52 | -1.69E-46 |
| MS.gene051975 | MS.gene62501 | 0.841716 | 4.01E-58 | -1.69E-46 |
| MS.gene052242 | MS.gene62501 | -0.82771 | 1.35E-54 | -1.69E-46 |
| MS.gene052307 | MS.gene62501 | 0.809791 | 1.59E-50 | -1.69E-46 |
| MS.gene052434 | MS.gene62501 | 0.824892 | 6.31E-54 | -1.69E-46 |
| MS.gene052609 | MS.gene62501 | 0.858986 | 5.65E-63 | -1.69E-46 |
| MS.gene052776 | MS.gene62501 | 0.822376 | 2.45E-53 | -1.69E-46 |
| MS.gene052932 | MS.gene62501 | 0.805293 | 1.43E-49 | -1.69E-46 |
| MS.gene052994 | MS.gene62501 | 0.820888 | 5.41E-53 | -1.69E-46 |
| MS.gene053058 | MS.gene62501 | 0.834458 | 2.96E-56 | -1.69E-46 |
| MS.gene053079 | MS.gene62501 | 0.806486 | 8.04E-50 | -1.69E-46 |
| MS.gene053726 | MS.gene62501 | 0.807245 | 5.55E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene62501 | 0.814019 | 1.91E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene62501 | 0.802371 | 5.79E-49 | -1.69E-46 |
| MS.gene055311 | MS.gene62501 | 0.855266 | 7.08E-62 | -1.69E-46 |
| MS.gene055313 | MS.gene62501 | 0.845802 | 3.24E-59 | -1.69E-46 |
| MS.gene055314 | MS.gene62501 | 0.830628 | 2.64E-55 | -1.69E-46 |
| MS.gene055513 | MS.gene62501 | 0.827425 | 1.58E-54 | -1.69E-46 |
| MS.gene055603 | MS.gene62501 | 0.81389 | 2.04E-51 | -1.69E-46 |
| MS.gene055623 | MS.gene62501 | 0.821601 | 3.70E-53 | -1.69E-46 |
| MS.gene055864 | MS.gene62501 | 0.83979 | 1.28E-57 | -1.69E-46 |
| MS.gene056045 | MS.gene62501 | 0.825972 | 3.50E-54 | -1.69E-46 |
| MS.gene056097 | MS.gene62501 | 0.844204 | 8.74E-59 | -1.69E-46 |
| MS.gene05615 | MS.gene62501 | 0.806966 | 6.36E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene62501 | 0.855566 | 5.79E-62 | -1.69E-46 |
| MS.gene056403 | MS.gene62501 | 0.816431 | 5.55E-52 | -1.69E-46 |
| MS.gene056605 | MS.gene62501 | 0.805032 | 1.62E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene62501 | 0.822126 | 2.80E-53 | -1.69E-46 |
| MS.gene056787 | MS.gene62501 | 0.860479 | 2.01E-63 | -1.69E-46 |
| MS.gene056986 | MS.gene62501 | 0.826961 | 2.04E-54 | -1.69E-46 |
| MS.gene057034 | MS.gene62501 | 0.807369 | 5.23E-50 | -1.69E-46 |
| MS.gene057360 | MS.gene62501 | 0.837578 | 4.79E-57 | -1.69E-46 |
| MS.gene058054 | MS.gene62501 | 0.806876 | 6.65E-50 | -1.69E-46 |
| MS.gene058126 | MS.gene62501 | 0.80556 | 1.26E-49 | -1.69E-46 |
| MS.gene058289 | MS.gene62501 | 0.822616 | 2.15E-53 | -1.69E-46 |
| MS.gene058292 | MS.gene62501 | 0.839284 | 1.74E-57 | -1.69E-46 |
| MS.gene058638 | MS.gene62501 | 0.845429 | 4.08E-59 | -1.69E-46 |
| MS.gene058639 | MS.gene62501 | 0.848394 | 6.30E-60 | -1.69E-46 |
| MS.gene058758 | MS.gene62501 | 0.846567 | 2.00E-59 | -1.69E-46 |
| MS.gene059007 | MS.gene62501 | 0.838444 | 2.87E-57 | -1.69E-46 |
| MS.gene059171 | MS.gene62501 | 0.807744 | 4.35E-50 | -1.69E-46 |
| MS.gene059360 | MS.gene62501 | 0.829947 | 3.87E-55 | -1.69E-46 |
| MS.gene059965 | MS.gene62501 | 0.812906 | 3.35E-51 | -1.69E-46 |
| MS.gene06010 | MS.gene62501 | 0.837664 | 4.55E-57 | -1.69E-46 |
| MS.gene060296 | MS.gene62501 | 0.839929 | 1.18E-57 | -1.69E-46 |
| MS.gene060297 | MS.gene62501 | 0.800188 | 1.62E-48 | -1.69E-46 |
| MS.gene060400 | MS.gene62501 | 0.817733 | 2.83E-52 | -1.69E-46 |
| MS.gene060637 | MS.gene62501 | 0.823289 | 1.50E-53 | -1.69E-46 |
| MS.gene060933 | MS.gene62501 | 0.840923 | 6.49E-58 | -1.69E-46 |
| MS.gene060937 | MS.gene62501 | 0.807574 | 4.73E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene38292 | MS.gene62501 | PPI |
| MS.gene053130 | MS.gene62501 | PPI |
| MS.gene053131 | MS.gene62501 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62501.t1 | MTR_2g064415 | 99.535 | 215 | 1 | 0 | 1 | 215 | 1 | 215 | 1.68e-162 | 449 |
| MS.gene62501.t1 | MTR_4g116980 | 29.375 | 160 | 111 | 2 | 58 | 215 | 36 | 195 | 4.82e-21 | 87.8 |
| MS.gene62501.t1 | MTR_4g008540 | 24.719 | 178 | 132 | 2 | 39 | 214 | 17 | 194 | 1.99e-16 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62501.t1 | AT3G12760 | 75.926 | 216 | 51 | 1 | 1 | 215 | 1 | 216 | 3.75e-125 | 354 |
| MS.gene62501.t1 | AT1G15860 | 26.705 | 176 | 127 | 2 | 42 | 215 | 20 | 195 | 6.52e-20 | 84.7 |
| MS.gene62501.t1 | AT1G15860 | 26.705 | 176 | 127 | 2 | 42 | 215 | 20 | 195 | 6.52e-20 | 84.7 |
| MS.gene62501.t1 | AT1G15860 | 26.486 | 185 | 125 | 3 | 42 | 215 | 20 | 204 | 8.41e-18 | 79.3 |
Find 48 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTGAAGGCTTACAATCTT+TGG | 0.213937 | 2.1:-31253205 | MS.gene62501:CDS |
| ATTCTTTGTGCAGCATAAAT+TGG | 0.217465 | 2.1:-31259832 | MS.gene62501:intron |
| GGATACATGGTCTCAGCTTT+TGG | 0.269497 | 2.1:-31251782 | MS.gene62501:CDS |
| CTGGCTTTGGATACAGCTAT+TGG | 0.290596 | 2.1:-31252006 | MS.gene62501:CDS |
| ATTGAAGGCTTACAATCTTT+GGG | 0.290934 | 2.1:-31253204 | MS.gene62501:CDS |
| GAGATATATAACTTTGCTTT+TGG | 0.322910 | 2.1:-31252887 | MS.gene62501:CDS |
| CAGCAGTTCGTAACGATAAC+TGG | 0.329636 | 2.1:-31259786 | MS.gene62501:CDS |
| TTATTGCAGGATTGATTCTC+TGG | 0.338350 | 2.1:-31253106 | MS.gene62501:intron |
| GGCTTATTACCTGCAAGAAT+TGG | 0.342538 | 2.1:+31251929 | None:intergenic |
| TTCTTTGTGCAGCATAAATT+GGG | 0.361871 | 2.1:-31259831 | MS.gene62501:intron |
| TTCAGCTCAGAGCGCATATA+TGG | 0.364877 | 2.1:+31253063 | None:intergenic |
| GAGCTATACAATAGATATAA+AGG | 0.381421 | 2.1:-31258667 | MS.gene62501:intron |
| GGCATAACAAAGCAATATCT+AGG | 0.382983 | 2.1:-31251804 | MS.gene62501:CDS |
| ACATTGGTGCCAATTCTTGC+AGG | 0.391466 | 2.1:-31251938 | MS.gene62501:intron |
| TGGCTTTGGATACAGCTATT+GGG | 0.410722 | 2.1:-31252005 | MS.gene62501:CDS |
| TGCTGAGAAGCAGTGGCCTC+TGG | 0.419876 | 2.1:-31251965 | MS.gene62501:CDS |
| CAGCGAGAAAGTGGCAATGC+AGG | 0.455285 | 2.1:-31258785 | MS.gene62501:CDS |
| CCTCCTTTGCAATGATATCC+AGG | 0.475584 | 2.1:-31256851 | MS.gene62501:intron |
| CTTTCATGGCACATGAAGGC+TGG | 0.477640 | 2.1:-31253258 | MS.gene62501:CDS |
| TAAATTGGGGAGAGGTCATC+GGG | 0.477715 | 2.1:-31259817 | MS.gene62501:CDS |
| CAGTTATCGTTACGAACTGC+TGG | 0.487867 | 2.1:+31259787 | None:intergenic |
| GCTAGTGATTGGAATCTTGA+AGG | 0.498024 | 2.1:-31258754 | MS.gene62501:CDS |
| AGTGGCAATGCAGGCTCTGA+AGG | 0.498625 | 2.1:-31258776 | MS.gene62501:CDS |
| CTTCACTGATACTAGGCACT+TGG | 0.508982 | 2.1:-31258692 | MS.gene62501:CDS |
| CACTGATACTAGGCACTTGG+AGG | 0.516875 | 2.1:-31258689 | MS.gene62501:CDS |
| AGGCTCTGAAGGCTAGTGAT+TGG | 0.526944 | 2.1:-31258765 | MS.gene62501:CDS |
| TTAGAACCTTCACTGATACT+AGG | 0.536113 | 2.1:-31258699 | MS.gene62501:CDS |
| TGAAGGTTCTAAGCTGAGGC+TGG | 0.538587 | 2.1:+31258710 | None:intergenic |
| ATAAATTGGGGAGAGGTCAT+CGG | 0.545004 | 2.1:-31259818 | MS.gene62501:CDS |
| AGTTCTTTCATGGCACATGA+AGG | 0.546918 | 2.1:-31253262 | MS.gene62501:CDS |
| TGGATACAGCTATTGGGATG+TGG | 0.552347 | 2.1:-31251999 | MS.gene62501:CDS |
| TTGAAGGCTTACAATCTTTG+GGG | 0.561512 | 2.1:-31253203 | MS.gene62501:intron |
| AAGCAATATCTAGGGATACA+TGG | 0.569704 | 2.1:-31251795 | MS.gene62501:CDS |
| TATTATTTGCTGAGAAGCAG+TGG | 0.570737 | 2.1:-31251972 | MS.gene62501:CDS |
| GTGCAGCATAAATTGGGGAG+AGG | 0.579013 | 2.1:-31259825 | MS.gene62501:intron |
| GCATAACAAAGCAATATCTA+GGG | 0.579769 | 2.1:-31251803 | MS.gene62501:CDS |
| CATACCATTACTATGTCTTG+AGG | 0.583443 | 2.1:+31254351 | None:intergenic |
| ATTGATATGATTTATGCAGA+CGG | 0.595709 | 2.1:-31256880 | MS.gene62501:CDS |
| CCTGGATATCATTGCAAAGG+AGG | 0.603718 | 2.1:+31256851 | None:intergenic |
| TCTTTGTGCAGCATAAATTG+GGG | 0.617535 | 2.1:-31259830 | MS.gene62501:intron |
| CTGGATATCATTGCAAAGGA+GGG | 0.626647 | 2.1:+31256852 | None:intergenic |
| CAACCTGGATATCATTGCAA+AGG | 0.631060 | 2.1:+31256848 | None:intergenic |
| GGATCCTCAAGACATAGTAA+TGG | 0.646312 | 2.1:-31254355 | MS.gene62501:intron |
| CAAGTGCCTAGTATCAGTGA+AGG | 0.648036 | 2.1:+31258693 | None:intergenic |
| TCAGTGAAGGTTCTAAGCTG+AGG | 0.649173 | 2.1:+31258706 | None:intergenic |
| AGTGGCCTCTGGTTGAACAT+TGG | 0.651904 | 2.1:-31251954 | MS.gene62501:CDS |
| TGGCACCAATGTTCAACCAG+AGG | 0.679329 | 2.1:+31251949 | None:intergenic |
| TCGTAACGATAACTGGAGCG+AGG | 0.712120 | 2.1:-31259779 | MS.gene62501:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 31251771 | 31260691 | 31251771 | ID=MS.gene62501 |
| chr2.1 | mRNA | 31251771 | 31260691 | 31251771 | ID=MS.gene62501.t1;Parent=MS.gene62501 |
| chr2.1 | exon | 31260689 | 31260691 | 31260689 | ID=MS.gene62501.t1.exon1;Parent=MS.gene62501.t1 |
| chr2.1 | CDS | 31260689 | 31260691 | 31260689 | ID=cds.MS.gene62501.t1;Parent=MS.gene62501.t1 |
| chr2.1 | exon | 31259780 | 31259841 | 31259780 | ID=MS.gene62501.t1.exon2;Parent=MS.gene62501.t1 |
| chr2.1 | CDS | 31259780 | 31259841 | 31259780 | ID=cds.MS.gene62501.t1;Parent=MS.gene62501.t1 |
| chr2.1 | exon | 31258668 | 31258804 | 31258668 | ID=MS.gene62501.t1.exon3;Parent=MS.gene62501.t1 |
| chr2.1 | CDS | 31258668 | 31258804 | 31258668 | ID=cds.MS.gene62501.t1;Parent=MS.gene62501.t1 |
| chr2.1 | exon | 31256852 | 31256910 | 31256852 | ID=MS.gene62501.t1.exon4;Parent=MS.gene62501.t1 |
| chr2.1 | CDS | 31256852 | 31256910 | 31256852 | ID=cds.MS.gene62501.t1;Parent=MS.gene62501.t1 |
| chr2.1 | exon | 31254356 | 31254379 | 31254356 | ID=MS.gene62501.t1.exon5;Parent=MS.gene62501.t1 |
| chr2.1 | CDS | 31254356 | 31254379 | 31254356 | ID=cds.MS.gene62501.t1;Parent=MS.gene62501.t1 |
| chr2.1 | exon | 31253204 | 31253286 | 31253204 | ID=MS.gene62501.t1.exon6;Parent=MS.gene62501.t1 |
| chr2.1 | CDS | 31253204 | 31253286 | 31253204 | ID=cds.MS.gene62501.t1;Parent=MS.gene62501.t1 |
| chr2.1 | exon | 31253055 | 31253119 | 31253055 | ID=MS.gene62501.t1.exon7;Parent=MS.gene62501.t1 |
| chr2.1 | CDS | 31253055 | 31253119 | 31253055 | ID=cds.MS.gene62501.t1;Parent=MS.gene62501.t1 |
| chr2.1 | exon | 31252871 | 31252920 | 31252871 | ID=MS.gene62501.t1.exon8;Parent=MS.gene62501.t1 |
| chr2.1 | CDS | 31252871 | 31252920 | 31252871 | ID=cds.MS.gene62501.t1;Parent=MS.gene62501.t1 |
| chr2.1 | exon | 31251939 | 31252040 | 31251939 | ID=MS.gene62501.t1.exon9;Parent=MS.gene62501.t1 |
| chr2.1 | CDS | 31251939 | 31252040 | 31251939 | ID=cds.MS.gene62501.t1;Parent=MS.gene62501.t1 |
| chr2.1 | exon | 31251771 | 31251830 | 31251771 | ID=MS.gene62501.t1.exon10;Parent=MS.gene62501.t1 |
| chr2.1 | CDS | 31251771 | 31251830 | 31251771 | ID=cds.MS.gene62501.t1;Parent=MS.gene62501.t1 |
| Gene Sequence |
| Protein sequence |
