AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63719

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63719.t1 MTR_2g075830 84.071 339 15 1 1 300 3 341 0.0 573
MS.gene63719.t1 MTR_5g022560 68.622 341 65 2 2 300 4 344 3.50e-171 478
MS.gene63719.t1 MTR_4g107930 68.915 341 63 3 3 300 5 345 3.85e-169 473
MS.gene63719.t1 MTR_4g079770 68.605 344 61 6 2 300 4 345 5.97e-166 464
MS.gene63719.t1 MTR_4g047610 68.713 342 62 4 2 300 4 343 1.59e-161 453
MS.gene63719.t1 MTR_4g079470 68.098 326 58 5 19 300 22 345 7.80e-156 439
MS.gene63719.t1 MTR_4g079800 67.372 331 62 5 14 300 17 345 3.52e-152 429
MS.gene63719.t1 MTR_4g080700 56.013 316 97 4 25 300 27 340 2.85e-124 358
MS.gene63719.t1 MTR_4g080730 55.696 316 98 4 25 300 27 340 1.18e-123 357
MS.gene63719.t1 MTR_4g080360 47.605 334 128 5 12 300 11 342 9.00e-108 317
MS.gene63719.t1 MTR_4g104370 59.846 259 91 5 43 298 94 342 3.78e-107 316
MS.gene63719.t1 MTR_7g078600 51.290 310 107 6 29 298 31 336 2.64e-106 313
MS.gene63719.t1 MTR_2g083930 46.087 345 133 5 1 298 4 342 3.54e-106 313
MS.gene63719.t1 MTR_3g075390 63.717 226 81 1 73 298 119 343 5.79e-105 310
MS.gene63719.t1 MTR_7g079230 47.077 325 129 5 14 298 13 334 7.64e-102 301
MS.gene63719.t1 MTR_4g079440 60.793 227 88 1 72 298 118 343 8.46e-101 299
MS.gene63719.t1 MTR_3g116080 49.032 310 114 6 31 299 41 347 5.59e-98 292
MS.gene63719.t1 MTR_1g107550 52.281 285 125 6 21 298 74 354 8.64e-98 296
MS.gene63719.t1 MTR_2g090890 44.073 329 141 5 13 299 9 336 4.63e-96 287
MS.gene63719.t1 MTR_4g094918 45.562 338 136 8 8 299 6 341 5.47e-94 282
MS.gene63719.t1 MTR_1g018840 53.232 263 112 4 37 298 108 360 3.00e-93 284
MS.gene63719.t1 MTR_1g018840 53.232 263 112 4 37 298 108 360 3.24e-93 283
MS.gene63719.t1 MTR_3g102220 60.092 218 84 3 82 298 145 360 8.25e-91 278
MS.gene63719.t1 MTR_3g112430 52.896 259 106 4 51 299 89 341 1.40e-86 266
MS.gene63719.t1 MTR_2g015850 55.505 218 93 3 81 298 149 362 6.29e-75 234
MS.gene63719.t1 MTR_2g016180 50.450 222 103 4 81 299 138 355 4.78e-72 230
MS.gene63719.t1 MTR_2g016270 50.450 222 103 4 81 299 123 340 5.59e-72 229
MS.gene63719.t1 MTR_7g078630 43.866 269 119 6 19 259 686 950 6.59e-65 219
MS.gene63719.t1 MTR_1g023810 44.397 232 120 7 69 297 36 261 8.77e-62 197
MS.gene63719.t1 MTR_2g067900 39.557 316 130 10 36 297 15 323 3.20e-59 192
MS.gene63719.t1 MTR_2g015920 35.244 349 156 14 5 291 10 350 1.57e-57 189
MS.gene63719.t1 MTR_1g023210 38.909 275 122 8 35 291 106 352 4.94e-57 187
MS.gene63719.t1 MTR_4g065077 40.152 264 134 8 43 299 110 356 2.23e-56 186
MS.gene63719.t1 MTR_8g086470 40.152 264 134 8 43 299 110 356 2.62e-56 186
MS.gene63719.t1 MTR_4g071060 43.111 225 108 5 81 291 130 348 2.76e-55 183
MS.gene63719.t1 MTR_4g125300 44.105 229 114 7 78 299 127 348 4.84e-55 182
MS.gene63719.t1 MTR_2g064475 45.614 228 103 11 82 299 135 351 3.85e-52 175
MS.gene63719.t1 MTR_2g067560 46.606 221 98 11 82 294 116 324 1.75e-49 176
MS.gene63719.t1 MTR_1g094135 39.914 233 112 7 77 291 136 358 6.50e-49 167
MS.gene63719.t1 MTR_2g015930 32.113 355 166 14 2 291 6 350 2.51e-48 165
MS.gene63719.t1 MTR_2g066980 31.073 354 143 14 8 299 4 318 5.09e-44 153
MS.gene63719.t1 MTR_7g078580 64.286 98 34 1 201 298 4 100 1.25e-42 143
MS.gene63719.t1 MTR_2g015840 32.166 314 148 10 6 299 3 271 2.94e-40 141
MS.gene63719.t1 MTR_4g072790 38.785 214 97 7 81 291 130 312 8.15e-39 139
MS.gene63719.t1 MTR_7g079130 65.556 90 31 0 209 298 7 96 4.60e-38 130
MS.gene63719.t1 MTR_4g072340 37.387 222 101 7 77 291 126 316 2.02e-37 135
MS.gene63719.t1 MTR_4g073660 38.073 218 97 7 81 291 129 315 4.03e-37 135
MS.gene63719.t1 MTR_1g025450 38.095 189 90 7 69 252 42 208 1.37e-33 129
MS.gene63719.t1 MTR_7g078310 49.541 109 53 1 69 175 139 247 1.82e-29 113
MS.gene63719.t1 MTR_2g016380 43.590 117 61 3 186 299 21 135 2.22e-26 104
MS.gene63719.t1 MTR_3g029560 28.638 213 122 6 93 299 49 237 3.79e-23 95.9
MS.gene63719.t1 MTR_8g087510 45.038 131 65 5 123 251 23 148 3.25e-22 91.7
MS.gene63719.t1 MTR_7g111060 29.778 225 130 7 88 291 113 330 7.73e-22 94.4
MS.gene63719.t1 MTR_7g111050 29.741 232 135 7 81 291 103 327 8.95e-21 91.3
MS.gene63719.t1 MTR_7g079260 52.778 72 18 1 225 296 7 62 8.18e-19 80.5
MS.gene63719.t1 MTR_2g047960 47.674 86 44 1 90 175 6 90 4.71e-18 78.2
MS.gene63719.t1 MTR_7g111050 27.723 202 122 6 81 261 98 296 5.86e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63719.t1 AT5G50260 65.044 226 78 1 73 298 117 341 5.28e-108 318
MS.gene63719.t1 AT5G45890 47.647 340 124 8 12 299 8 345 5.12e-105 310
MS.gene63719.t1 AT3G48340 61.181 237 90 2 62 298 108 342 7.58e-104 308
MS.gene63719.t1 AT4G35350 54.545 264 104 4 37 299 104 352 5.94e-99 295
MS.gene63719.t1 AT3G48350 61.233 227 86 2 74 299 118 343 2.73e-98 293
MS.gene63719.t1 AT1G06260 47.305 334 131 6 8 298 10 341 4.74e-98 292
MS.gene63719.t1 AT4G36880 55.556 261 103 6 43 299 110 361 2.08e-96 289
MS.gene63719.t1 AT2G27420 45.797 345 134 5 8 300 5 348 1.15e-95 286
MS.gene63719.t1 AT5G43060 60.829 217 83 2 82 298 138 352 3.46e-94 286
MS.gene63719.t1 AT1G20850 57.826 230 95 2 70 299 126 353 8.51e-94 282
MS.gene63719.t1 AT2G34080 43.678 348 145 8 1 300 1 345 9.51e-94 281
MS.gene63719.t1 AT3G49340 43.695 341 140 5 8 300 5 341 2.60e-93 280
MS.gene63719.t1 AT1G47128 56.223 233 98 4 68 298 121 351 5.97e-93 283
MS.gene63719.t1 AT3G19390 45.509 334 138 7 8 299 14 345 1.47e-92 282
MS.gene63719.t1 AT3G19400 53.516 256 107 6 48 299 101 348 5.41e-91 275
MS.gene63719.t1 AT1G29090 43.059 353 145 8 1 300 6 355 1.04e-88 269
MS.gene63719.t1 AT4G23520 54.113 231 99 5 72 298 121 348 5.11e-88 267
MS.gene63719.t1 AT4G11310 50.575 261 115 6 41 298 102 351 7.91e-87 264
MS.gene63719.t1 AT1G29080 42.407 349 142 10 8 300 1 346 5.97e-86 261
MS.gene63719.t1 AT1G09850 56.221 217 93 2 82 298 118 332 1.42e-84 261
MS.gene63719.t1 AT4G11320 50.410 244 113 4 56 298 122 358 4.76e-84 257
MS.gene63719.t1 AT4G11320 50.410 244 113 4 56 298 122 358 4.76e-84 257
MS.gene63719.t1 AT3G43960 49.345 229 110 5 74 298 119 345 3.24e-71 224
MS.gene63719.t1 AT4G35350 52.525 198 78 4 37 233 104 286 7.64e-66 208
MS.gene63719.t1 AT1G29110 36.137 321 142 7 27 299 28 333 3.55e-63 202
MS.gene63719.t1 AT3G48350 60.870 161 61 2 74 233 118 277 1.65e-61 197
MS.gene63719.t1 AT2G21430 36.000 300 163 7 6 291 65 349 2.19e-56 186
MS.gene63719.t1 AT3G45310 44.105 229 116 7 77 300 136 357 2.46e-56 186
MS.gene63719.t1 AT3G19400 51.053 190 82 5 48 233 101 283 5.58e-56 183
MS.gene63719.t1 AT5G60360 44.643 224 112 7 82 300 141 357 1.21e-54 181
MS.gene63719.t1 AT4G39090 43.111 225 108 5 81 291 134 352 1.74e-54 181
MS.gene63719.t1 AT4G16190 40.873 252 130 5 54 291 112 358 4.73e-54 180
MS.gene63719.t1 AT3G45310 43.668 229 116 8 77 300 136 356 9.97e-54 179
MS.gene63719.t1 AT5G60360 44.196 224 112 8 82 300 141 356 3.88e-52 175
MS.gene63719.t1 AT5G60360 43.379 219 116 6 82 295 141 356 1.20e-51 174
MS.gene63719.t1 AT3G54940 36.704 267 141 7 43 291 98 354 3.70e-49 167
MS.gene63719.t1 AT3G54940 36.111 180 107 2 43 215 98 276 6.51e-33 122
MS.gene63719.t1 AT1G02305 28.614 332 179 13 9 291 11 333 2.53e-25 103
MS.gene63719.t1 AT4G01610 30.268 261 153 9 56 295 82 334 3.04e-23 98.2
MS.gene63719.t1 AT4G01610 29.885 261 154 9 56 295 82 334 4.74e-22 95.1
MS.gene63719.t1 AT1G02300 30.332 211 121 7 101 291 146 350 1.50e-18 85.5

Find 80 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTGCTAAGTCCATTATTT+TGG 0.159503 2.1:+23828401 None:intergenic
TATAAAAGTGGTGTCTTTAC+TGG 0.196559 2.1:-23828196 MS.gene63719:CDS
ATTTGCACTGTTTCTTTGCT+TGG 0.249332 2.1:-23829494 MS.gene63719:CDS
AGCTCTGCTGCTACCATTTC+CGG 0.251167 2.1:-23828322 MS.gene63719:CDS
TAAGTTGACTACAGGAAATT+TGG 0.271144 2.1:-23828521 MS.gene63719:CDS
GAATTCATTATCCAAAATAA+TGG 0.292338 2.1:-23828412 MS.gene63719:CDS
TTTGCACTGTTTCTTTGCTT+GGG 0.336850 2.1:-23829493 MS.gene63719:CDS
ACAATATAGCAAAGTCTATA+AGG 0.340865 2.1:-23829395 MS.gene63719:CDS
CGTAGCTAATCAACCGGTTT+CGG 0.341078 2.1:-23828254 MS.gene63719:CDS
AATGCCTGCTCATCATTTGC+GGG 0.355488 2.1:+23828286 None:intergenic
AGGTTGTGAGGGTGGTCTTA+TGG 0.376813 2.1:-23828446 MS.gene63719:CDS
GACATTCTCGTACCCGGAAA+TGG 0.380302 2.1:+23828309 None:intergenic
TGTTTCTTTGCTTGGGATTG+TGG 0.383785 2.1:-23829486 MS.gene63719:CDS
GGTGTAGCTGTTGTGGGTTA+TGG 0.386579 2.1:-23828148 MS.gene63719:CDS
CAATGCCTGCTCATCATTTG+CGG 0.390262 2.1:+23828285 None:intergenic
AATTATGCAGTTGGATAAGA+TGG 0.402093 2.1:+23827995 None:intergenic
TACCCTTATCAAGGTGTTGA+TGG 0.404971 2.1:-23828370 MS.gene63719:CDS
TATATTTGTAGGATGTTGTT+GGG 0.406693 2.1:-23828578 MS.gene63719:intron
GCATCAATGGCTACCGAAAC+CGG 0.418768 2.1:+23828241 None:intergenic
GCTCTGCTGCTACCATTTCC+GGG 0.439218 2.1:-23828321 MS.gene63719:CDS
CTATATTTGTAGGATGTTGT+TGG 0.443719 2.1:-23828579 MS.gene63719:intron
GAATTCGTGGGGAACTCAAT+GGG 0.449733 2.1:-23828089 MS.gene63719:CDS
TTCCATCAACACCTTGATAA+GGG 0.450919 2.1:+23828368 None:intergenic
GCTGTTGTGGGTTATGGTGT+TGG 0.453735 2.1:-23828142 MS.gene63719:CDS
GATCATGGTGTAGCTGTTGT+GGG 0.460675 2.1:-23828154 MS.gene63719:CDS
GGAACTCAATGGGGTGAAGA+AGG 0.462980 2.1:-23828079 MS.gene63719:CDS
GTTCCATCAACACCTTGATA+AGG 0.464383 2.1:+23828367 None:intergenic
GCAACTAGAAACAAATTCAA+TGG 0.464776 2.1:-23829251 MS.gene63719:CDS
TGCTCATGCCTCTCATACAT+AGG 0.466056 2.1:+23829427 None:intergenic
AAATCTTATAATTATGCAGT+TGG 0.468251 2.1:+23827986 None:intergenic
GGATCATGGTGTAGCTGTTG+TGG 0.482554 2.1:-23828155 MS.gene63719:CDS
GTCAGAGCCACTAGCATCAA+TGG 0.482715 2.1:+23828228 None:intergenic
AGTCAACTTATGAATTCCTT+CGG 0.488253 2.1:+23828534 None:intergenic
CATTCGGATGCAAAGGGGTG+TGG 0.489197 2.1:-23828053 MS.gene63719:CDS
GGAATTCATAAGTTGACTAC+AGG 0.503372 2.1:-23828529 MS.gene63719:CDS
TACCATCTGCAGTGGATTGG+AGG 0.508009 2.1:-23829175 MS.gene63719:CDS
GGGGTGAAGAAGGGTACATT+CGG 0.514049 2.1:-23828069 MS.gene63719:CDS
AGAATTCGTGGGGAACTCAA+TGG 0.514840 2.1:-23828090 MS.gene63719:CDS
AAAAGCCGTAGCTAATCAAC+CGG 0.515012 2.1:-23828260 MS.gene63719:CDS
AGGGTACATTCGGATGCAAA+GGG 0.518284 2.1:-23828059 MS.gene63719:CDS
ATCTGCAGTGGATTGGAGGC+AGG 0.519791 2.1:-23829171 MS.gene63719:CDS
TGCGGGGACATTCTCGTACC+CGG 0.524526 2.1:+23828303 None:intergenic
TGTCCCCGCAAATGATGAGC+AGG 0.529226 2.1:-23828290 MS.gene63719:CDS
AAGGGTACATTCGGATGCAA+AGG 0.541356 2.1:-23828060 MS.gene63719:CDS
ATTGGTTGGTGAAGAATTCG+TGG 0.543072 2.1:-23828102 MS.gene63719:CDS
AAATTCAATGGATATTTGCA+TGG 0.546885 2.1:-23829239 MS.gene63719:CDS
AATAGACACACTTACCGCAT+TGG 0.548111 2.1:+23829117 None:intergenic
TTGGTTGGTGAAGAATTCGT+GGG 0.553024 2.1:-23828101 MS.gene63719:CDS
GAAGCTGAATACCCTTATCA+AGG 0.560075 2.1:-23828379 MS.gene63719:CDS
TGTGGAACTGAGTTGGATCA+TGG 0.564256 2.1:-23828169 MS.gene63719:CDS
GAACTCAATGGGGTGAAGAA+GGG 0.565034 2.1:-23828078 MS.gene63719:CDS
GCTCATGCCTCTCATACATA+GGG 0.569140 2.1:+23829428 None:intergenic
GTGAAGATGAGACTGAGTAT+TGG 0.569553 2.1:-23828120 MS.gene63719:CDS
TCTGCGGTTGCATCAACCGA+AGG 0.569767 2.1:-23828550 MS.gene63719:CDS
ATGTACCATCTGCAGTGGAT+TGG 0.570020 2.1:-23829178 MS.gene63719:CDS
CGAAACCGGTTGATTAGCTA+CGG 0.573070 2.1:+23828255 None:intergenic
TCGGTAGCCATTGATGCTAG+TGG 0.574292 2.1:-23828235 MS.gene63719:CDS
TGCCTCCAATCCACTGCAGA+TGG 0.579586 2.1:+23829173 None:intergenic
TCTATAAGGATGCACATGAA+AGG 0.584380 2.1:-23829381 MS.gene63719:CDS
TGGTTCATGTGGAACTGAGT+TGG 0.588339 2.1:-23828176 MS.gene63719:CDS
TAAGACCACCCTCACAACCT+TGG 0.592405 2.1:+23828449 None:intergenic
TGAGAGGCATGAGCAATGGA+TGG 0.593973 2.1:-23829419 MS.gene63719:CDS
AGGGGTGTGGATGCTTCAGA+AGG 0.602547 2.1:-23828040 MS.gene63719:CDS
GAGAGGCATGAGCAATGGAT+GGG 0.613645 2.1:-23829418 MS.gene63719:CDS
TGTATGAGAGGCATGAGCAA+TGG 0.614507 2.1:-23829423 MS.gene63719:CDS
GCAGTGGATTGGAGGCAGGA+AGG 0.619784 2.1:-23829167 MS.gene63719:CDS
AGATGAGACTGAGTATTGGT+TGG 0.624949 2.1:-23828116 MS.gene63719:CDS
GAAGACCAAGGTTGTGAGGG+TGG 0.626235 2.1:-23828454 MS.gene63719:CDS
AAAATGACCCTATGTATGAG+AGG 0.630864 2.1:-23829435 MS.gene63719:CDS
GGTGTCTTTACTGGTTCATG+TGG 0.637602 2.1:-23828187 MS.gene63719:CDS
TGGTGAAGACCAAGGTTGTG+AGG 0.640947 2.1:-23828458 MS.gene63719:CDS
GGTGAAGACCAAGGTTGTGA+GGG 0.650031 2.1:-23828457 MS.gene63719:CDS
ATGCCTGCTCATCATTTGCG+GGG 0.658912 2.1:+23828287 None:intergenic
CTTGTTGATTGTGACACAAG+TGG 0.664457 2.1:-23828478 MS.gene63719:CDS
AATTCGTGGGGAACTCAATG+GGG 0.670782 2.1:-23828088 MS.gene63719:CDS
TAGTGATGTACCATCTGCAG+TGG 0.678103 2.1:-23829183 MS.gene63719:CDS
GATGCTTCAGAAGGACTATG+TGG 0.707697 2.1:-23828031 MS.gene63719:CDS
TGGTTGGTGAAGAATTCGTG+GGG 0.721704 2.1:-23828100 MS.gene63719:CDS
GGGTACATTCGGATGCAAAG+GGG 0.734527 2.1:-23828058 MS.gene63719:CDS
GACACAAGTGGTGAAGACCA+AGG 0.761833 2.1:-23828466 MS.gene63719:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAATAGTAACTTTTTT+TGG - chr2.1:23828623-23828642 MS.gene63719:intron 10.0%
!!! AAAAAATAGTAACTTTTTTT+GGG - chr2.1:23828624-23828643 MS.gene63719:intron 10.0%
!! CTAACAATTTCATAAAAAAA+GGG - chr2.1:23828717-23828736 MS.gene63719:intron 15.0%
!! TCTAACAATTTCATAAAAAA+AGG - chr2.1:23828716-23828735 MS.gene63719:intron 15.0%
!! ATTTATACAATATGCGAATT+TGG - chr2.1:23828590-23828609 MS.gene63719:intron 20.0%
!! CATATAACAACTATATTTGT+AGG - chr2.1:23828928-23828947 MS.gene63719:intron 20.0%
!! GAATTCATTATCCAAAATAA+TGG - chr2.1:23829105-23829124 MS.gene63719:intron 20.0%
!! TCCTATATATCAAAGAAAAT+TGG + chr2.1:23828524-23828543 None:intergenic 20.0%
!!! ATTTTGGATAATGAATTCAA+AGG + chr2.1:23829103-23829122 None:intergenic 20.0%
!!! TCCAATTTTCTTTGATATAT+AGG - chr2.1:23828520-23828539 MS.gene63719:CDS 20.0%
! AAATTCAATGGATATTTGCA+TGG - chr2.1:23828278-23828297 MS.gene63719:CDS 25.0%
! ACAATATAGCAAAGTCTATA+AGG - chr2.1:23828122-23828141 MS.gene63719:CDS 25.0%
! TATATTTGTAGGATGTTGTT+GGG - chr2.1:23828939-23828958 MS.gene63719:intron 25.0%
! TTTATGCGTAAGATAAAGTT+AGG - chr2.1:23828439-23828458 MS.gene63719:CDS 25.0%
!! GACTTCCAATTTTATAAAAG+TGG - chr2.1:23829309-23829328 MS.gene63719:intron 25.0%
!!! AGACACCACTTTTATAAAAT+TGG + chr2.1:23829317-23829336 None:intergenic 25.0%
ACATCTATTATTACTCCCTA+TGG + chr2.1:23828547-23828566 None:intergenic 30.0%
AGTCAACTTATGAATTCCTT+CGG + chr2.1:23828986-23829005 None:intergenic 30.0%
ATAGGGAGTAATAATAGATG+TGG - chr2.1:23828546-23828565 MS.gene63719:CDS 30.0%
ATATGAAATTGTTAGACCGA+GGG + chr2.1:23828913-23828932 None:intergenic 30.0%
CTATATTTGTAGGATGTTGT+TGG - chr2.1:23828938-23828957 MS.gene63719:intron 30.0%
CTTTGATATATAGGACCATA+GGG - chr2.1:23828529-23828548 MS.gene63719:CDS 30.0%
GCAACTAGAAACAAATTCAA+TGG - chr2.1:23828266-23828285 MS.gene63719:CDS 30.0%
TATAAAAGTGGTGTCTTTAC+TGG - chr2.1:23829321-23829340 MS.gene63719:intron 30.0%
TATATGAAATTGTTAGACCG+AGG + chr2.1:23828914-23828933 None:intergenic 30.0%
TCTTTGATATATAGGACCAT+AGG - chr2.1:23828528-23828547 MS.gene63719:CDS 30.0%
! TAAGTTGACTACAGGAAATT+TGG - chr2.1:23828996-23829015 MS.gene63719:intron 30.0%
!! TTTTGGGTAGATAGACAAAA+TGG - chr2.1:23828640-23828659 MS.gene63719:intron 30.0%
AAAATGACCCTATGTATGAG+AGG - chr2.1:23828082-23828101 MS.gene63719:CDS 35.0%
TAAAGTTGTTGTCATGTGAC+TGG + chr2.1:23828871-23828890 None:intergenic 35.0%
TATGAAATTGTTAGACCGAG+GGG + chr2.1:23828912-23828931 None:intergenic 35.0%
TCTATAAGGATGCACATGAA+AGG - chr2.1:23828136-23828155 MS.gene63719:CDS 35.0%
TCTTGTGTAAAAACAGGGTA+AGG + chr2.1:23828817-23828836 None:intergenic 35.0%
TTCATAAAAAAAGGGCAACC+CGG - chr2.1:23828725-23828744 MS.gene63719:intron 35.0%
TTCCATCAACACCTTGATAA+GGG + chr2.1:23829152-23829171 None:intergenic 35.0%
! ATTTGCACTGTTTCTTTGCT+TGG - chr2.1:23828023-23828042 MS.gene63719:CDS 35.0%
! CAGTGCTAAGTCCATTATTT+TGG + chr2.1:23829119-23829138 None:intergenic 35.0%
! CATGAAAGGCAAAAACGTTT+TGG - chr2.1:23828150-23828169 MS.gene63719:CDS 35.0%
! GGAATTCATAAGTTGACTAC+AGG - chr2.1:23828988-23829007 MS.gene63719:intron 35.0%
! TTCTAGTTGCTGTAAACTCA+TGG + chr2.1:23828256-23828275 None:intergenic 35.0%
! TTTGCACTGTTTCTTTGCTT+GGG - chr2.1:23828024-23828043 MS.gene63719:CDS 35.0%
!!! TTACCCTGTTTTTACACAAG+AGG - chr2.1:23828816-23828835 MS.gene63719:intron 35.0%
AAAAGCCGTAGCTAATCAAC+CGG - chr2.1:23829257-23829276 MS.gene63719:intron 40.0%
AATAGACACACTTACCGCAT+TGG + chr2.1:23828403-23828422 None:intergenic 40.0%
ACAGCCTCTTGTGTAAAAAC+AGG + chr2.1:23828823-23828842 None:intergenic 40.0%
CAATCCTTGGTCACATTGTT+CGG - chr2.1:23828693-23828712 MS.gene63719:intron 40.0%
CAGCCTCTTGTGTAAAAACA+GGG + chr2.1:23828822-23828841 None:intergenic 40.0%
CGTACAATACACCAAATGGT+GGG + chr2.1:23828791-23828810 None:intergenic 40.0%
GAAGCTGAATACCCTTATCA+AGG - chr2.1:23829138-23829157 MS.gene63719:CDS 40.0%
GTGAAAAATCAAGGCCAATG+CGG - chr2.1:23828386-23828405 MS.gene63719:CDS 40.0%
GTGAAGATGAGACTGAGTAT+TGG - chr2.1:23829397-23829416 MS.gene63719:CDS 40.0%
GTGACACCAGTGAAAAATCA+AGG - chr2.1:23828377-23828396 MS.gene63719:CDS 40.0%
GTTCCATCAACACCTTGATA+AGG + chr2.1:23829153-23829172 None:intergenic 40.0%
TGTTTCTTTGCTTGGGATTG+TGG - chr2.1:23828031-23828050 MS.gene63719:CDS 40.0%
! CTTGTTGATTGTGACACAAG+TGG - chr2.1:23829039-23829058 MS.gene63719:intron 40.0%
!! AGATGAGACTGAGTATTGGT+TGG - chr2.1:23829401-23829420 MS.gene63719:CDS 40.0%
!! ATTGGTTGGTGAAGAATTCG+TGG - chr2.1:23829415-23829434 MS.gene63719:CDS 40.0%
!! TACCCTTATCAAGGTGTTGA+TGG - chr2.1:23829147-23829166 MS.gene63719:CDS 40.0%
!! TTGGTTGGTGAAGAATTCGT+GGG - chr2.1:23829416-23829435 MS.gene63719:CDS 40.0%
!!! CATTGGCCTTGATTTTTCAC+TGG + chr2.1:23828386-23828405 None:intergenic 40.0%
AATGCCTGCTCATCATTTGC+GGG + chr2.1:23829234-23829253 None:intergenic 45.0%
AATTCGTGGGGAACTCAATG+GGG - chr2.1:23829429-23829448 MS.gene63719:CDS 45.0%
ACACACTAGTGAATGAGTCG+AGG - chr2.1:23828668-23828687 MS.gene63719:intron 45.0%
AGAATTCGTGGGGAACTCAA+TGG - chr2.1:23829427-23829446 MS.gene63719:CDS 45.0%
AGGGTACATTCGGATGCAAA+GGG - chr2.1:23829458-23829477 MS.gene63719:CDS 45.0%
ATGAGTCGAGGTTCAATCCT+TGG - chr2.1:23828680-23828699 MS.gene63719:intron 45.0%
ATGTACCATCTGCAGTGGAT+TGG - chr2.1:23828339-23828358 MS.gene63719:CDS 45.0%
CAATGCCTGCTCATCATTTG+CGG + chr2.1:23829235-23829254 None:intergenic 45.0%
GAACTCAATGGGGTGAAGAA+GGG - chr2.1:23829439-23829458 MS.gene63719:CDS 45.0%
GAATTCGTGGGGAACTCAAT+GGG - chr2.1:23829428-23829447 MS.gene63719:CDS 45.0%
GATCATGGTGTAGCTGTTGT+GGG - chr2.1:23829363-23829382 MS.gene63719:intron 45.0%
GATGCTTCAGAAGGACTATG+TGG - chr2.1:23829486-23829505 MS.gene63719:CDS 45.0%
GCGTACAATACACCAAATGG+TGG + chr2.1:23828792-23828811 None:intergenic 45.0%
GCTCATGCCTCTCATACATA+GGG + chr2.1:23828092-23828111 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG + chr2.1:23828795-23828814 None:intergenic 45.0%
GTTGTTGTCATGTGACTGGA+AGG + chr2.1:23828867-23828886 None:intergenic 45.0%
TAGACCGAACAATGTGACCA+AGG + chr2.1:23828700-23828719 None:intergenic 45.0%
TAGTGATGTACCATCTGCAG+TGG - chr2.1:23828334-23828353 MS.gene63719:CDS 45.0%
TGCTCATGCCTCTCATACAT+AGG + chr2.1:23828093-23828112 None:intergenic 45.0%
TGGTTCATGTGGAACTGAGT+TGG - chr2.1:23829341-23829360 MS.gene63719:intron 45.0%
! AAGGGTACATTCGGATGCAA+AGG - chr2.1:23829457-23829476 MS.gene63719:CDS 45.0%
! ATGTTGTTGGGCGTTTTCTG+CGG - chr2.1:23828951-23828970 MS.gene63719:intron 45.0%
! CGAAACCGGTTGATTAGCTA+CGG + chr2.1:23829265-23829284 None:intergenic 45.0%
! CGTAGCTAATCAACCGGTTT+CGG - chr2.1:23829263-23829282 MS.gene63719:intron 45.0%
! TGTATGAGAGGCATGAGCAA+TGG - chr2.1:23828094-23828113 MS.gene63719:CDS 45.0%
! TGTGGAACTGAGTTGGATCA+TGG - chr2.1:23829348-23829367 MS.gene63719:intron 45.0%
! TTACACAAGAGGCTGTTTCC+AGG - chr2.1:23828827-23828846 MS.gene63719:intron 45.0%
!! GGTGTCTTTACTGGTTCATG+TGG - chr2.1:23829330-23829349 MS.gene63719:intron 45.0%
!! TGGTTGGTGAAGAATTCGTG+GGG - chr2.1:23829417-23829436 MS.gene63719:CDS 45.0%
!! ATAAATAAATCTTATAAATT+AGG - chr2.1:23828203-23828222 MS.gene63719:CDS 5.0%
ACTAAAGCTCCCGCATATGC+GGG - chr2.1:23828751-23828770 MS.gene63719:intron 50.0%
AGCTCTGCTGCTACCATTTC+CGG - chr2.1:23829195-23829214 MS.gene63719:CDS 50.0%
ATGCCTGCTCATCATTTGCG+GGG + chr2.1:23829233-23829252 None:intergenic 50.0%
CAACTTTACCAGTTGCGCCA+AGG - chr2.1:23828883-23828902 MS.gene63719:intron 50.0%
CACTAAAGCTCCCGCATATG+CGG - chr2.1:23828750-23828769 MS.gene63719:intron 50.0%
GACACAAGTGGTGAAGACCA+AGG - chr2.1:23829051-23829070 MS.gene63719:intron 50.0%
GACATTCTCGTACCCGGAAA+TGG + chr2.1:23829211-23829230 None:intergenic 50.0%
GCATCAATGGCTACCGAAAC+CGG + chr2.1:23829279-23829298 None:intergenic 50.0%
GGAACTCAATGGGGTGAAGA+AGG - chr2.1:23829438-23829457 MS.gene63719:CDS 50.0%
GGATCATGGTGTAGCTGTTG+TGG - chr2.1:23829362-23829381 MS.gene63719:intron 50.0%
GGGTACATTCGGATGCAAAG+GGG - chr2.1:23829459-23829478 MS.gene63719:CDS 50.0%
GGTGAAGACCAAGGTTGTGA+GGG - chr2.1:23829060-23829079 MS.gene63719:intron 50.0%
GTCAGAGCCACTAGCATCAA+TGG + chr2.1:23829292-23829311 None:intergenic 50.0%
GTCATGTGACTGGAAGGTCA+CGG + chr2.1:23828861-23828880 None:intergenic 50.0%
TAAGACCACCCTCACAACCT+TGG + chr2.1:23829071-23829090 None:intergenic 50.0%
TCATGTGACTGGAAGGTCAC+GGG + chr2.1:23828860-23828879 None:intergenic 50.0%
TCGGTAGCCATTGATGCTAG+TGG - chr2.1:23829282-23829301 MS.gene63719:intron 50.0%
TGGTGAAGACCAAGGTTGTG+AGG - chr2.1:23829059-23829078 MS.gene63719:intron 50.0%
! AGGTTGTGAGGGTGGTCTTA+TGG - chr2.1:23829071-23829090 MS.gene63719:intron 50.0%
! GAGAGGCATGAGCAATGGAT+GGG - chr2.1:23828099-23828118 MS.gene63719:CDS 50.0%
! GCTGTTGTGGGTTATGGTGT+TGG - chr2.1:23829375-23829394 MS.gene63719:CDS 50.0%
! GGGGTGAAGAAGGGTACATT+CGG - chr2.1:23829448-23829467 MS.gene63719:CDS 50.0%
! GGTGTAGCTGTTGTGGGTTA+TGG - chr2.1:23829369-23829388 MS.gene63719:intron 50.0%
! TGAGAGGCATGAGCAATGGA+TGG - chr2.1:23828098-23828117 MS.gene63719:CDS 50.0%
!! TACCATCTGCAGTGGATTGG+AGG - chr2.1:23828342-23828361 MS.gene63719:CDS 50.0%
AAGGTCACGGGTTCAAGTCC+TGG + chr2.1:23828848-23828867 None:intergenic 55.0%
CATTCGGATGCAAAGGGGTG+TGG - chr2.1:23829464-23829483 MS.gene63719:CDS 55.0%
CTAAAGCTCCCGCATATGCG+GGG - chr2.1:23828752-23828771 MS.gene63719:intron 55.0%
GAAGACCAAGGTTGTGAGGG+TGG - chr2.1:23829063-23829082 MS.gene63719:intron 55.0%
GCTCTGCTGCTACCATTTCC+GGG - chr2.1:23829196-23829215 MS.gene63719:CDS 55.0%
GTTAGACCGAGGGGTAACCT+TGG + chr2.1:23828903-23828922 None:intergenic 55.0%
TCTGCGGTTGCATCAACCGA+AGG - chr2.1:23828967-23828986 MS.gene63719:intron 55.0%
TGCCTCCAATCCACTGCAGA+TGG + chr2.1:23828347-23828366 None:intergenic 55.0%
TGTCCCCGCAAATGATGAGC+AGG - chr2.1:23829227-23829246 MS.gene63719:CDS 55.0%
! AGGGGTGTGGATGCTTCAGA+AGG - chr2.1:23829477-23829496 MS.gene63719:CDS 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG + chr2.1:23828747-23828766 None:intergenic 55.0%
!! ATCTGCAGTGGATTGGAGGC+AGG - chr2.1:23828346-23828365 MS.gene63719:CDS 55.0%
AGGGGTAACCTTGGCGCAAC+TGG + chr2.1:23828894-23828913 None:intergenic 60.0%
GTTGCGCCAAGGTTACCCCT+CGG - chr2.1:23828894-23828913 MS.gene63719:intron 60.0%
TGCGGGGACATTCTCGTACC+CGG + chr2.1:23829217-23829236 None:intergenic 60.0%
! CAAATGGTGGGACCCCTTCC+CGG + chr2.1:23828779-23828798 None:intergenic 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG + chr2.1:23828746-23828765 None:intergenic 60.0%
!! GCAGTGGATTGGAGGCAGGA+AGG - chr2.1:23828350-23828369 MS.gene63719:CDS 60.0%
CATATGCGGGGTCCGGGAAG+GGG - chr2.1:23828764-23828783 MS.gene63719:intron 65.0%
CGGGAAGGGGTCCCACCATT+TGG - chr2.1:23828777-23828796 MS.gene63719:intron 65.0%
CTTCCCGGACCCCGCATATG+CGG + chr2.1:23828764-23828783 None:intergenic 65.0%
GCATATGCGGGGTCCGGGAA+GGG - chr2.1:23828763-23828782 MS.gene63719:intron 65.0%
TTCCCGGACCCCGCATATGC+GGG + chr2.1:23828763-23828782 None:intergenic 65.0%
CGCATATGCGGGGTCCGGGA+AGG - chr2.1:23828762-23828781 MS.gene63719:intron 70.0%
CTCCCGCATATGCGGGGTCC+GGG - chr2.1:23828758-23828777 MS.gene63719:intron 70.0%
GCTCCCGCATATGCGGGGTC+CGG - chr2.1:23828757-23828776 MS.gene63719:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 23827997 23829542 23827997 ID=MS.gene63719
chr2.1 mRNA 23827997 23829542 23827997 ID=MS.gene63719.t1;Parent=MS.gene63719
chr2.1 exon 23829374 23829542 23829374 ID=MS.gene63719.t1.exon1;Parent=MS.gene63719.t1
chr2.1 CDS 23829374 23829542 23829374 ID=cds.MS.gene63719.t1;Parent=MS.gene63719.t1
chr2.1 exon 23829132 23829272 23829132 ID=MS.gene63719.t1.exon2;Parent=MS.gene63719.t1
chr2.1 CDS 23829132 23829272 23829132 ID=cds.MS.gene63719.t1;Parent=MS.gene63719.t1
chr2.1 exon 23827997 23828589 23827997 ID=MS.gene63719.t1.exon3;Parent=MS.gene63719.t1
chr2.1 CDS 23827997 23828589 23827997 ID=cds.MS.gene63719.t1;Parent=MS.gene63719.t1
Gene Sequence

>MS.gene63719

ATGAATAATCAATTGCATTATATTTCATTTGCACTGTTTCTTTGCTTGGGATTGTGGAGTTTTCAAGCTACATCTCGAACTCTTCAAAATGACCCTATGTATGAGAGGCATGAGCAATGGATGGGACAATATAGCAAAGTCTATAAGGATGCACATGAAAGGCAAAAACGTTTTGGAATATTCAAAGAAAATGTGAATTACATTGAATAAATAAATCTTATAAATTAGGAATTAATCATTTTGCTGATCTTAGCAACCATGAGTTTACAGCAACTAGAAACAAATTCAATGGATATTTGCATGGCTCAAGCATAACTACTTTTAAGTACATAAATGTTAGTGATGTACCATCTGCAGTGGATTGGAGGCAGGAAGGAGCAGTGACACCAGTGAAAAATCAAGGCCAATGCGGTAAGTGTGTCTATTTAATACTTTCTCTGATTTTATGCGTAAGATAAAGTTAGGTCAACAATAGTTGATGTATCTTGTTCAAATTTTGAATCAGATACATAAACTTTATTGATCCAATTTTCTTTGATATATAGGACCATAGGGAGTAATAATAGATGTGGTTACTTTATTTTATTCTTTTAATTTATACAATATGCGAATTTGGTTTCTCAATTAAAAAAATAGTAACTTTTTTTGGGTAGATAGACAAAATGGCTTGCACACACTAGTGAATGAGTCGAGGTTCAATCCTTGGTCACATTGTTCGGTCTAACAATTTCATAAAAAAAGGGCAACCCGGTGCACTAAAGCTCCCGCATATGCGGGGTCCGGGAAGGGGTCCCACCATTTGGTGTATTGTACGCAGCCTTACCCTGTTTTTACACAAGAGGCTGTTTCCAGGACTTGAACCCGTGACCTTCCAGTCACATGACAACAACTTTACCAGTTGCGCCAAGGTTACCCCTCGGTCTAACAATTTCATATAACAACTATATTTGTAGGATGTTGTTGGGCGTTTTCTGCGGTTGCATCAACCGAAGGAATTCATAAGTTGACTACAGGAAATTTGGTCTCTTTATCAGAGCAAGAACTTGTTGATTGTGACACAAGTGGTGAAGACCAAGGTTGTGAGGGTGGTCTTATGGATGATGCCTTTGAATTCATTATCCAAAATAATGGACTTAGCACTGAAGCTGAATACCCTTATCAAGGTGTTGATGGAACATGCAATAAAACTGAAGTATGCAGCTCTGCTGCTACCATTTCCGGGTACGAGAATGTCCCCGCAAATGATGAGCAGGCATTGCAAAAAGCCGTAGCTAATCAACCGGTTTCGGTAGCCATTGATGCTAGTGGCTCTGACTTCCAATTTTATAAAAGTGGTGTCTTTACTGGTTCATGTGGAACTGAGTTGGATCATGGTGTAGCTGTTGTGGGTTATGGTGTTGGTGAAGATGAGACTGAGTATTGGTTGGTGAAGAATTCGTGGGGAACTCAATGGGGTGAAGAAGGGTACATTCGGATGCAAAGGGGTGTGGATGCTTCAGAAGGACTATGTGGCATTGCAATGCAACCATCTTATCCAACTGCATAA
Protein sequence

>MS.gene63719.t1

MNNQLHYISFALFLCLGLWSFQATSRTLQNDPMYERHEQWMGQYSKVYKDAHERQKPTRNKFNGYLHGSSITTFKYINVSDVPSAVDWRQEGAVTPVKNQGQCGCCWAFSAVASTEGIHKLTTGNLVSLSEQELVDCDTSGEDQGCEGGLMDDAFEFIIQNNGLSTEAEYPYQGVDGTCNKTEVCSSAATISGYENVPANDEQALQKAVANQPVSVAIDASGSDFQFYKSGVFTGSCGTELDHGVAVVGYGVGEDETEYWLVKNSWGTQWGEEGYIRMQRGVDASEGLCGIAMQPSYPTA