AlfalfaGEDB | MS.gene65895

M. sativa cultivar XinJiangDaYe / MS.gene65895

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004553 GO:0005975 GO:0008150 GO:0008152 GO:0016787 GO:0016798 GO:0044238 GO:0071704
PFAM: PF01915.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65895.t1	XP_003615003.1	96.2	80	3	0	1	80	499	578	5.20E-35	156.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65895.t1	Q9SGZ5	70.0	80	24	0	1	80	489	568	5.9e-27	121.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65895.t1	G7KA59	96.2	80	3	0	1	80	499	578	3.8e-35	156.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene65895	0.82625	3.01E-54	-1.69E-46
MS.gene049424	MS.gene65895	0.812806	3.52E-51	-1.69E-46
MS.gene049435	MS.gene65895	0.800036	1.74E-48	-1.69E-46
MS.gene050442	MS.gene65895	0.813488	2.50E-51	-1.69E-46
MS.gene050605	MS.gene65895	0.849448	3.21E-60	-1.69E-46
MS.gene050750	MS.gene65895	0.810865	9.32E-51	-1.69E-46
MS.gene050953	MS.gene65895	0.830916	2.24E-55	-1.69E-46
MS.gene051368	MS.gene65895	0.825883	3.68E-54	-1.69E-46
MS.gene051370	MS.gene65895	0.832584	8.70E-56	-1.69E-46
MS.gene051452	MS.gene65895	0.800616	1.33E-48	-1.69E-46
MS.gene051535	MS.gene65895	0.811186	7.94E-51	-1.69E-46
MS.gene052426	MS.gene65895	0.826338	2.87E-54	-1.69E-46
MS.gene052500	MS.gene65895	0.828274	9.85E-55	-1.69E-46
MS.gene052502	MS.gene65895	0.828112	1.08E-54	-1.69E-46
MS.gene052824	MS.gene65895	0.805736	1.16E-49	-1.69E-46
MS.gene052826	MS.gene65895	0.826459	2.68E-54	-1.69E-46
MS.gene052867	MS.gene65895	0.809301	2.02E-50	-1.69E-46
MS.gene05288	MS.gene65895	0.824942	6.14E-54	-1.69E-46
MS.gene05289	MS.gene65895	0.816498	5.36E-52	-1.69E-46
MS.gene052954	MS.gene65895	0.843792	1.13E-58	-1.69E-46
MS.gene053006	MS.gene65895	0.810086	1.37E-50	-1.69E-46
MS.gene053080	MS.gene65895	0.812037	5.19E-51	-1.69E-46
MS.gene053166	MS.gene65895	0.828542	8.48E-55	-1.69E-46
MS.gene054405	MS.gene65895	0.823305	1.49E-53	-1.69E-46
MS.gene054559	MS.gene65895	0.804915	1.72E-49	-1.69E-46
MS.gene054657	MS.gene65895	0.837957	3.83E-57	-1.69E-46
MS.gene054658	MS.gene65895	0.808508	2.99E-50	-1.69E-46
MS.gene054994	MS.gene65895	0.813025	3.15E-51	-1.69E-46
MS.gene05510	MS.gene65895	0.83885	2.25E-57	-1.69E-46
MS.gene055623	MS.gene65895	0.811654	6.28E-51	-1.69E-46
MS.gene055701	MS.gene65895	0.833467	5.24E-56	-1.69E-46
MS.gene055841	MS.gene65895	0.808644	2.80E-50	-1.69E-46
MS.gene056506	MS.gene65895	0.835214	1.91E-56	-1.69E-46
MS.gene057060	MS.gene65895	0.802826	4.67E-49	-1.69E-46
MS.gene057208	MS.gene65895	0.806702	7.24E-50	-1.69E-46
MS.gene057392	MS.gene65895	0.819697	1.01E-52	-1.69E-46
MS.gene057693	MS.gene65895	0.812636	3.84E-51	-1.69E-46
MS.gene057869	MS.gene65895	0.801564	8.49E-49	-1.69E-46
MS.gene058146	MS.gene65895	0.833036	6.71E-56	-1.69E-46
MS.gene058435	MS.gene65895	0.823022	1.73E-53	-1.69E-46
MS.gene058548	MS.gene65895	0.859475	4.03E-63	-1.69E-46
MS.gene058549	MS.gene65895	0.809881	1.52E-50	-1.69E-46
MS.gene058595	MS.gene65895	0.877523	5.87E-69	-1.69E-46
MS.gene058979	MS.gene65895	0.821195	4.59E-53	-1.69E-46
MS.gene059317	MS.gene65895	0.805342	1.40E-49	-1.69E-46
MS.gene059550	MS.gene65895	0.804254	2.36E-49	-1.69E-46
MS.gene05979	MS.gene65895	0.833608	4.84E-56	-1.69E-46
MS.gene059842	MS.gene65895	0.806789	6.94E-50	-1.69E-46
MS.gene060011	MS.gene65895	0.831805	1.36E-55	-1.69E-46
MS.gene060310	MS.gene65895	0.847216	1.33E-59	-1.69E-46
MS.gene060322	MS.gene65895	0.80499	1.66E-49	-1.69E-46
MS.gene060683	MS.gene65895	0.808538	2.95E-50	-1.69E-46
MS.gene060685	MS.gene65895	0.80809	3.67E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65895.t1	MTR_5g062370	93.827	81	5	1	81	501	581	1.33e-47	159
MS.gene65895.t1	MTR_5g062320	95.062	81	4	1	81	499	579	1.52e-47	161
MS.gene65895.t1	MTR_5g062370	93.827	81	5	1	81	501	581	4.17e-47	160
MS.gene65895.t1	MTR_5g062430	92.593	81	6	1	81	501	581	2.51e-46	157
MS.gene65895.t1	MTR_5g062650	82.716	81	14	1	81	504	584	4.78e-41	143
MS.gene65895.t1	MTR_3g074060	80.247	81	16	1	81	495	575	1.49e-39	139
MS.gene65895.t1	MTR_2g034720	60.494	81	32	1	81	483	563	1.45e-29	110
MS.gene65895.t1	MTR_7g109390	60.000	85	34	1	85	485	569	2.44e-29	109
MS.gene65895.t1	MTR_1g087240	59.036	83	34	1	83	480	562	4.12e-28	106
MS.gene65895.t1	MTR_5g030860	56.250	80	35	2	81	497	576	2.01e-25	98.6
MS.gene65895.t1	MTR_2g008240	54.321	81	37	1	81	500	580	1.76e-24	96.3
MS.gene65895.t1	MTR_1g106860	55.000	80	36	2	81	488	567	4.74e-24	94.7
MS.gene65895.t1	MTR_4g094285	49.383	81	41	1	81	499	579	3.38e-22	89.7

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65895.t1	AT1G78060	69.136	81	25	1	81	489	569	1.60e-34	124
MS.gene65895.t1	AT5G10560	66.250	80	27	2	81	508	587	2.00e-31	115
MS.gene65895.t1	AT5G49360	60.494	81	32	1	81	491	571	3.22e-30	112
MS.gene65895.t1	AT1G02640	60.494	81	32	1	81	487	567	5.72e-29	108
MS.gene65895.t1	AT5G64570	61.728	81	31	1	81	503	583	1.67e-28	107
MS.gene65895.t1	AT5G64570	61.728	81	31	1	81	503	583	2.58e-28	107
MS.gene65895.t1	AT5G64570	61.728	81	31	1	81	345	425	3.44e-28	106
MS.gene65895.t1	AT5G09700	61.728	81	31	1	81	246	326	6.33e-28	105
MS.gene65895.t1	AT5G09730	59.259	81	33	1	81	493	573	8.71e-26	99.8
MS.gene65895.t1	AT3G19620	54.321	81	37	1	81	489	569	1.04e-24	96.7

Find 26 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTAAGAAGCCTGTTATTT+TGG	0.174390	5.2:-71667763	MS.gene65895:CDS
GCTAAGAATAATGACAAAAT+TGG	0.212120	5.2:-71667705	MS.gene65895:CDS
ACAAAATTGGAGGCATCATT+TGG	0.281644	5.2:-71667692	MS.gene65895:CDS
TATGTGTGTTTATTTGTTCA+TGG	0.334180	5.2:+71667581	None:intergenic
CACAAAATTACCAAAATAAC+AGG	0.354358	5.2:+71667753	None:intergenic
ATCATTTGGGCTGGTTATCC+TGG	0.383440	5.2:-71667678	MS.gene65895:CDS
GCTGGTTATCCTGGTGAAAC+TGG	0.384326	5.2:-71667669	MS.gene65895:CDS
TGGTGGAAGGGCTCTGGCTC+AGG	0.399913	5.2:-71667649	MS.gene65895:CDS
ATAAGTTCCTGTTGCTTACC+TGG	0.420423	5.2:+71667804	None:intergenic
TGAAACTGGTGGAAGGGCTC+TGG	0.429489	5.2:-71667655	MS.gene65895:CDS
GGCTCTGGCTCAGGTTGTGT+TGG	0.437265	5.2:-71667640	MS.gene65895:CDS
CAAAATTGGAGGCATCATTT+GGG	0.439628	5.2:-71667691	MS.gene65895:CDS
TGTGTTGGTGACTATAACCC+AGG	0.464048	5.2:-71667625	MS.gene65895:CDS
TTAGCAAAAGTAATATCAAC+AGG	0.467973	5.2:+71667723	None:intergenic
AGAGTTACCAGGTAAGCAAC+AGG	0.474539	5.2:-71667811	MS.gene65895:CDS
GTGTTTCCGTCTCTTGACTT+TGG	0.508660	5.2:+71667850	None:intergenic
AAGAATAATGACAAAATTGG+AGG	0.530513	5.2:-71667702	MS.gene65895:CDS
ATTGGAGGCATCATTTGGGC+TGG	0.536363	5.2:-71667687	MS.gene65895:CDS
GCCCTTCCACCAGTTTCACC+AGG	0.557917	5.2:+71667660	None:intergenic
ATCCTGGTGAAACTGGTGGA+AGG	0.570539	5.2:-71667662	MS.gene65895:CDS
AGAGATCATTTAGAGTTACC+AGG	0.579180	5.2:-71667822	MS.gene65895:CDS
TCCTGGTGAAACTGGTGGAA+GGG	0.585464	5.2:-71667661	MS.gene65895:CDS
GGTTATCCTGGTGAAACTGG+TGG	0.612813	5.2:-71667666	MS.gene65895:CDS
AAGTATAGAAGAAACTAACC+TGG	0.616192	5.2:+71667607	None:intergenic
ATTGGACCAAAGTCAAGAGA+CGG	0.639353	5.2:-71667856	MS.gene65895:CDS
AGTATAGAAGAAACTAACCT+GGG	0.669940	5.2:+71667608	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGAATAATGACAAAATTGG+AGG	-	chr5.2:71667749-71667768	MS.gene65895:CDS	25.0%
!	CACAAAATTACCAAAATAAC+AGG	+	chr5.2:71667701-71667720	None:intergenic	25.0%
!	GCTAAGAATAATGACAAAAT+TGG	-	chr5.2:71667746-71667765	MS.gene65895:CDS	25.0%
!	TTAGCAAAAGTAATATCAAC+AGG	+	chr5.2:71667731-71667750	None:intergenic	25.0%
!!!	GTTATTTTGGTAATTTTGTG+TGG	-	chr5.2:71667701-71667720	MS.gene65895:CDS	25.0%
	AAGTATAGAAGAAACTAACC+TGG	+	chr5.2:71667847-71667866	None:intergenic	30.0%
	AGTATAGAAGAAACTAACCT+GGG	+	chr5.2:71667846-71667865	None:intergenic	30.0%
!!	ATTTTGGTAATTTTGTGTGG+TGG	-	chr5.2:71667704-71667723	MS.gene65895:CDS	30.0%
!!	TTCTAAGAAGCCTGTTATTT+TGG	-	chr5.2:71667688-71667707	MS.gene65895:CDS	30.0%
!	AGAGATCATTTAGAGTTACC+AGG	-	chr5.2:71667629-71667648	MS.gene65895:CDS	35.0%
!!	ACAAAATTGGAGGCATCATT+TGG	-	chr5.2:71667759-71667778	MS.gene65895:CDS	35.0%
!!	CAAAATTGGAGGCATCATTT+GGG	-	chr5.2:71667760-71667779	MS.gene65895:CDS	35.0%
	ATTGGACCAAAGTCAAGAGA+CGG	-	chr5.2:71667595-71667614	MS.gene65895:CDS	40.0%
!	ATAAGTTCCTGTTGCTTACC+TGG	+	chr5.2:71667650-71667669	None:intergenic	40.0%
	AGAGTTACCAGGTAAGCAAC+AGG	-	chr5.2:71667640-71667659	MS.gene65895:CDS	45.0%
	GTGTTTCCGTCTCTTGACTT+TGG	+	chr5.2:71667604-71667623	None:intergenic	45.0%
!!	ATCATTTGGGCTGGTTATCC+TGG	-	chr5.2:71667773-71667792	MS.gene65895:CDS	45.0%
!!	TGTGTTGGTGACTATAACCC+AGG	-	chr5.2:71667826-71667845	MS.gene65895:CDS	45.0%
	ATCCTGGTGAAACTGGTGGA+AGG	-	chr5.2:71667789-71667808	MS.gene65895:CDS	50.0%
	GCTGGTTATCCTGGTGAAAC+TGG	-	chr5.2:71667782-71667801	MS.gene65895:CDS	50.0%
	GGTTATCCTGGTGAAACTGG+TGG	-	chr5.2:71667785-71667804	MS.gene65895:CDS	50.0%
	TCCTGGTGAAACTGGTGGAA+GGG	-	chr5.2:71667790-71667809	MS.gene65895:CDS	50.0%
!!	ATTGGAGGCATCATTTGGGC+TGG	-	chr5.2:71667764-71667783	MS.gene65895:CDS	50.0%
	TGAAACTGGTGGAAGGGCTC+TGG	-	chr5.2:71667796-71667815	MS.gene65895:CDS	55.0%
	GCCCTTCCACCAGTTTCACC+AGG	+	chr5.2:71667794-71667813	None:intergenic	60.0%
!	GGCTCTGGCTCAGGTTGTGT+TGG	-	chr5.2:71667811-71667830	MS.gene65895:CDS	60.0%
!	TGGTGGAAGGGCTCTGGCTC+AGG	-	chr5.2:71667802-71667821	MS.gene65895:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	71667590	71667883	71667590	ID=MS.gene65895
chr5.2	mRNA	71667590	71667883	71667590	ID=MS.gene65895.t1;Parent=MS.gene65895
chr5.2	exon	71667590	71667883	71667590	ID=MS.gene65895.t1.exon1;Parent=MS.gene65895.t1
chr5.2	CDS	71667590	71667883	71667590	ID=cds.MS.gene65895.t1;Parent=MS.gene65895.t1

Gene Sequence	>MS.gene65895 ATGGGATTGGACCAAAGTCAAGAGACGGAAACACGTGATAGAGATCATTTAGAGTTACCAGGTAAGCAACAGGAACTTATTAACAGTGTTGCTAAAGCTTCTAAGAAGCCTGTTATTTTGGTAATTTTGTGTGGTGGTCCTGTTGATATTACTTTTGCTAAGAATAATGACAAAATTGGAGGCATCATTTGGGCTGGTTATCCTGGTGAAACTGGTGGAAGGGCTCTGGCTCAGGTTGTGTTGGTGACTATAACCCAGGTTAGTTTCTTCTATACTTTATCCATGAACAAATAA
Protein sequence	>MS.gene65895.t1 MGLDQSQETETRDRDHLELPGKQQELINSVAKASKKPVILVILCGGPVDITFAKNNDKIGGIIWAGYPGETGGRALAQVVLVTITQVSFFYTLSMNK