AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65930.t1 MTR_1g042730 97.647 170 4 0 1 170 248 417 5.02e-121 348
MS.gene65930.t1 MTR_8g088160 50.538 93 37 6 1 88 226 314 2.03e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65930.t1 AT1G04420 74.556 169 43 0 2 170 244 412 1.04e-92 275

Find 36 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGCAAGCTATAGCTGTTC+TGG 0.272185 1.1:-26568122 None:intergenic
TTCTGGATACTGACAATCTT+TGG 0.280723 1.1:-26568105 None:intergenic
AAGCCAATGTTGCAATTCTT+TGG 0.310094 1.1:-26568959 None:intergenic
AGGAGTTAAACCATGTTTCT+TGG 0.319091 1.1:-26569404 None:intergenic
AATCCAAGCGCGAGCTGAAC+AGG 0.376331 1.1:-26569424 None:intergenic
TTCTTACCCGTGAAAGTGAT+TGG 0.390126 1.1:-26569099 None:intergenic
TGTGAGAGATCGTCCATTCA+TGG 0.397619 1.1:+26569447 MS.gene65930:CDS
CTAGGCGGCGGAACACTCTC+TGG 0.422546 1.1:+26568998 MS.gene65930:CDS
ATAATTGAACTCGCCATGAA+TGG 0.430321 1.1:-26569460 None:intergenic
ACTCCTGTTCAGCTCGCGCT+TGG 0.430695 1.1:+26569421 MS.gene65930:CDS
TTCATGGCGAGTTCAATTAT+CGG 0.446341 1.1:+26569463 MS.gene65930:CDS
CATCCAAAGAATTGCAACAT+TGG 0.453546 1.1:+26568956 MS.gene65930:CDS
CAATGTTGCAATTCTTTGGA+TGG 0.454759 1.1:-26568955 None:intergenic
ATTCCTGTCATGACTTCTGC+TGG 0.463780 1.1:-26569550 None:intergenic
GAATCTCTTCCCTGGCTACA+TGG 0.467731 1.1:+26569066 MS.gene65930:CDS
GATATAACCAATCACTTTCA+CGG 0.468306 1.1:+26569092 MS.gene65930:CDS
TTATATCTTTCCATGTAGCC+AGG 0.480910 1.1:-26569076 None:intergenic
AGAGTGTTCCGCCGCCTAGT+GGG 0.496927 1.1:-26568994 None:intergenic
CTTGAGTTGGCCAAGAAACA+TGG 0.517065 1.1:+26569394 MS.gene65930:CDS
GTCATGACTTCTGCTGGTAA+AGG 0.518717 1.1:-26569544 None:intergenic
TCCGCCGCCTAGTGGGGAAT+AGG 0.518898 1.1:-26568987 None:intergenic
GTCCATGCAGCTAAAGTTGA+GGG 0.519118 1.1:+26568078 MS.gene65930:CDS
TATAGTCTTTAAAGGATGCT+AGG 0.527210 1.1:-26569601 None:intergenic
AACTATTAAGTATCTTGAGT+TGG 0.528848 1.1:+26569381 MS.gene65930:CDS
GAGAGTGTTCCGCCGCCTAG+TGG 0.531448 1.1:-26568995 None:intergenic
GTCCCTCAACTTTAGCTGCA+TGG 0.544863 1.1:-26568080 None:intergenic
TCCTATTCCCCACTAGGCGG+CGG 0.552022 1.1:+26568986 MS.gene65930:CDS
ATATAACCAATCACTTTCAC+GGG 0.562310 1.1:+26569093 MS.gene65930:CDS
TTGCTATCCTATTCCCCACT+AGG 0.566323 1.1:+26568980 MS.gene65930:CDS
GGAAGACTGAATCTCTTCCC+TGG 0.567807 1.1:+26569058 MS.gene65930:CDS
CGTCCATGCAGCTAAAGTTG+AGG 0.578730 1.1:+26568077 MS.gene65930:CDS
TATATCTTTCCATGTAGCCA+GGG 0.587281 1.1:-26569075 None:intergenic
AATTATCGGTGCAACATCTG+TGG 0.606607 1.1:+26569477 MS.gene65930:CDS
TTACCAGCAGAAGTCATGAC+AGG 0.613222 1.1:+26569547 MS.gene65930:CDS
GAGTGTTCCGCCGCCTAGTG+GGG 0.617674 1.1:-26568993 None:intergenic
CTATCCTATTCCCCACTAGG+CGG 0.752007 1.1:+26568983 MS.gene65930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGGAAAATTCTTAAT+TGG - chr1.1:26569336-26569355 None:intergenic 15.0%
!!! AATGTAATACTTTTAAAACT+TGG - chr1.1:26568320-26568339 None:intergenic 15.0%
!!! ATGTAATACTTTTAAAACTT+GGG - chr1.1:26568319-26568338 None:intergenic 15.0%
!!! TATTTTTGCAAATTTCTTAA+TGG - chr1.1:26568832-26568851 None:intergenic 15.0%
!! AACCAAACATGAAAAAATAA+AGG - chr1.1:26569351-26569370 None:intergenic 20.0%
!!! TGTAATACTTTTAAAACTTG+GGG - chr1.1:26568318-26568337 None:intergenic 20.0%
!!! TTCCTTTATTTTTTCATGTT+TGG + chr1.1:26569346-26569365 MS.gene65930:intron 20.0%
! AACACGAATCATATACTATA+AGG - chr1.1:26568257-26568276 None:intergenic 25.0%
! AACCATACACTAAAAGTTTA+CGG - chr1.1:26568696-26568715 None:intergenic 25.0%
! AACTATTAAGTATCTTGAGT+TGG + chr1.1:26569381-26569400 MS.gene65930:CDS 25.0%
! ACAAATGATTACAGATAACA+TGG + chr1.1:26568450-26568469 MS.gene65930:intron 25.0%
! TAGAGAGTCCAAAATAAAAA+AGG + chr1.1:26568348-26568367 MS.gene65930:intron 25.0%
! TGAGTTTCTTTATCTTACAA+GGG + chr1.1:26568531-26568550 MS.gene65930:intron 25.0%
! TTATAATAACCAAACTGAAC+TGG - chr1.1:26569271-26569290 None:intergenic 25.0%
! TTGAGTTTCTTTATCTTACA+AGG + chr1.1:26568530-26568549 MS.gene65930:intron 25.0%
!!! TAATTCGGTTTTGAAAAAGA+CGG - chr1.1:26569156-26569175 None:intergenic 25.0%
!!! TTTTTTATTTTGGACTCTCT+AGG - chr1.1:26568349-26568368 None:intergenic 25.0%
!!! TTTTTTCATGTTTGGTTTTC+AGG + chr1.1:26569354-26569373 MS.gene65930:intron 25.0%
ATATAACCAATCACTTTCAC+GGG + chr1.1:26569093-26569112 MS.gene65930:CDS 30.0%
ATCACTTAATTCTGTATTGC+TGG + chr1.1:26568207-26568226 MS.gene65930:intron 30.0%
ATTGGTTATCTTGTTAACCA+TGG - chr1.1:26569318-26569337 None:intergenic 30.0%
CAGTTTGGTTATTATAACAC+TGG + chr1.1:26569274-26569293 MS.gene65930:intron 30.0%
CCAATTTATCCTTCAAACTT+GGG + chr1.1:26568618-26568637 MS.gene65930:intron 30.0%
GATATAACCAATCACTTTCA+CGG + chr1.1:26569092-26569111 MS.gene65930:CDS 30.0%
GATCATATTCATATGCACTT+TGG + chr1.1:26568640-26568659 MS.gene65930:intron 30.0%
GATTACAGATAACATGGTTT+AGG + chr1.1:26568456-26568475 MS.gene65930:intron 30.0%
TAAAAGTTTACGGATCACAT+TGG - chr1.1:26568686-26568705 None:intergenic 30.0%
TCATATACTATAAGGACAAC+AGG - chr1.1:26568249-26568268 None:intergenic 30.0%
TCCAATTTATCCTTCAAACT+TGG + chr1.1:26568617-26568636 MS.gene65930:intron 30.0%
TCTCACAATCAGCAATTTAT+AGG + chr1.1:26568555-26568574 MS.gene65930:intron 30.0%
TTATTTGAGCAGTTCAATTG+CGG - chr1.1:26569239-26569258 None:intergenic 30.0%
TTCAATGACTTTAAACCACT+CGG + chr1.1:26569207-26569226 MS.gene65930:intron 30.0%
! ATCCGTAAACTTTTAGTGTA+TGG + chr1.1:26568691-26568710 MS.gene65930:intron 30.0%
! ATGCTTTTACAACAACTGAA+CGG + chr1.1:26569521-26569540 MS.gene65930:CDS 30.0%
! CATCAATGTCTTCTTTTAGT+TGG - chr1.1:26569504-26569523 None:intergenic 30.0%
!! AATTCTAAAGCTGCAAAAAG+TGG + chr1.1:26569037-26569056 MS.gene65930:CDS 30.0%
!!! CAGGAAAGCCTTTTTTATTT+TGG - chr1.1:26568359-26568378 None:intergenic 30.0%
!!! TTGGACGTTTTTGAAGTTTA+AGG - chr1.1:26568667-26568686 None:intergenic 30.0%
AAGCCAATGTTGCAATTCTT+TGG - chr1.1:26568962-26568981 None:intergenic 35.0%
AATAACCAAACTGAACTGGA+TGG - chr1.1:26569267-26569286 None:intergenic 35.0%
AGCTTCTTCATGTTCTTAGT+TGG + chr1.1:26568897-26568916 MS.gene65930:intron 35.0%
AGGAGTTAAACCATGTTTCT+TGG - chr1.1:26569407-26569426 None:intergenic 35.0%
ATAATTGAACTCGCCATGAA+TGG - chr1.1:26569463-26569482 None:intergenic 35.0%
CAATGTTGCAATTCTTTGGA+TGG - chr1.1:26568958-26568977 None:intergenic 35.0%
CATCCAAAGAATTGCAACAT+TGG + chr1.1:26568956-26568975 MS.gene65930:CDS 35.0%
CCAAGTATTCAAAGATCTAC+AGG - chr1.1:26568378-26568397 None:intergenic 35.0%
CCCAAGTTTGAAGGATAAAT+TGG - chr1.1:26568621-26568640 None:intergenic 35.0%
CCTGTAGATCTTTGAATACT+TGG + chr1.1:26568375-26568394 MS.gene65930:intron 35.0%
GAATATGATCCCAAGTTTGA+AGG - chr1.1:26568630-26568649 None:intergenic 35.0%
TATATCTTTCCATGTAGCCA+GGG - chr1.1:26569078-26569097 None:intergenic 35.0%
TTATATCTTTCCATGTAGCC+AGG - chr1.1:26569079-26569098 None:intergenic 35.0%
TTCATGGCGAGTTCAATTAT+CGG + chr1.1:26569463-26569482 MS.gene65930:CDS 35.0%
TTCTGGATACTGACAATCTT+TGG - chr1.1:26568108-26568127 None:intergenic 35.0%
! GTTAACCATGGTTTAGAGTA+TGG - chr1.1:26569306-26569325 None:intergenic 35.0%
!! CTTGTAAGAAGTCGTTTTGA+AGG + chr1.1:26568141-26568160 MS.gene65930:CDS 35.0%
!!! TTTAGGATTTTAGTTGCCAG+AGG + chr1.1:26568473-26568492 MS.gene65930:intron 35.0%
AATTATCGGTGCAACATCTG+TGG + chr1.1:26569477-26569496 MS.gene65930:CDS 40.0%
AATTTACCCCACTCCAAGTT+AGG - chr1.1:26568800-26568819 None:intergenic 40.0%
ATTTACCCCACTCCAAGTTA+GGG - chr1.1:26568799-26568818 None:intergenic 40.0%
GCTAAAAATTCCCCAAAAGC+AGG - chr1.1:26568591-26568610 None:intergenic 40.0%
TCAGACCATACTCTAAACCA+TGG + chr1.1:26569298-26569317 MS.gene65930:intron 40.0%
TGCTGGTACGATGTCTAATT+CGG - chr1.1:26569171-26569190 None:intergenic 40.0%
TTCTTACCCGTGAAAGTGAT+TGG - chr1.1:26569102-26569121 None:intergenic 40.0%
TTGAAGGTGGCAAGAGAAAT+AGG + chr1.1:26568503-26568522 MS.gene65930:intron 40.0%
! AGTACAGTTCTTTTCTGACG+TGG + chr1.1:26568166-26568185 MS.gene65930:intron 40.0%
!! CATACATTCTCCTGCTTTTG+GGG + chr1.1:26568578-26568597 MS.gene65930:intron 40.0%
!! GCATACATTCTCCTGCTTTT+GGG + chr1.1:26568577-26568596 MS.gene65930:intron 40.0%
!! TGTTTTTGCAGTTGATCTCG+TGG + chr1.1:26568925-26568944 MS.gene65930:intron 40.0%
AACTTCACACCCGCTAACTT+AGG - chr1.1:26568874-26568893 None:intergenic 45.0%
ACTCCAAGTTAGGGTTCAAC+TGG - chr1.1:26568790-26568809 None:intergenic 45.0%
ACTTCACACCCGCTAACTTA+GGG - chr1.1:26568873-26568892 None:intergenic 45.0%
AGTCCAGTTGAACCCTAACT+TGG + chr1.1:26568784-26568803 MS.gene65930:intron 45.0%
AGTTGAACCCTAACTTGGAG+TGG + chr1.1:26568789-26568808 MS.gene65930:intron 45.0%
ATCGTACCAGCATGACTTCT+GGG + chr1.1:26569179-26569198 MS.gene65930:intron 45.0%
ATTCCTGTCATGACTTCTGC+TGG - chr1.1:26569553-26569572 None:intergenic 45.0%
CAAGCAAGCTATAGCTGTTC+TGG - chr1.1:26568125-26568144 None:intergenic 45.0%
CTGAACCATCCAGTTCAGTT+TGG + chr1.1:26569259-26569278 MS.gene65930:intron 45.0%
GTCATGACTTCTGCTGGTAA+AGG - chr1.1:26569547-26569566 None:intergenic 45.0%
GTCCATGCAGCTAAAGTTGA+GGG + chr1.1:26568078-26568097 MS.gene65930:CDS 45.0%
GTTGAACCCTAACTTGGAGT+GGG + chr1.1:26568790-26568809 MS.gene65930:intron 45.0%
TGTGAGAGATCGTCCATTCA+TGG + chr1.1:26569447-26569466 MS.gene65930:CDS 45.0%
TTACCAGCAGAAGTCATGAC+AGG + chr1.1:26569547-26569566 MS.gene65930:CDS 45.0%
TTGAACCCTAACTTGGAGTG+GGG + chr1.1:26568791-26568810 MS.gene65930:intron 45.0%
TTGCTATCCTATTCCCCACT+AGG + chr1.1:26568980-26568999 MS.gene65930:CDS 45.0%
! GGCATACATTCTCCTGCTTT+TGG + chr1.1:26568576-26568595 MS.gene65930:intron 45.0%
!! CTTGAGTTGGCCAAGAAACA+TGG + chr1.1:26569394-26569413 MS.gene65930:CDS 45.0%
!! TTTTAGTTGCCAGAGGTGCA+AGG + chr1.1:26568480-26568499 MS.gene65930:intron 45.0%
CACCATGACCCTAAGTTAGC+GGG + chr1.1:26568862-26568881 MS.gene65930:intron 50.0%
CATCGTACCAGCATGACTTC+TGG + chr1.1:26569178-26569197 MS.gene65930:intron 50.0%
CGTCCATGCAGCTAAAGTTG+AGG + chr1.1:26568077-26568096 MS.gene65930:CDS 50.0%
CTATCCTATTCCCCACTAGG+CGG + chr1.1:26568983-26569002 MS.gene65930:CDS 50.0%
GAATCTCTTCCCTGGCTACA+TGG + chr1.1:26569066-26569085 MS.gene65930:CDS 50.0%
GCACCATGACCCTAAGTTAG+CGG + chr1.1:26568861-26568880 MS.gene65930:intron 50.0%
GGAAGACTGAATCTCTTCCC+TGG + chr1.1:26569058-26569077 MS.gene65930:CDS 50.0%
GTCCCTCAACTTTAGCTGCA+TGG - chr1.1:26568083-26568102 None:intergenic 50.0%
!! CAATTGCGGTTTTGTCCGAG+TGG - chr1.1:26569225-26569244 None:intergenic 50.0%
AATCCAAGCGCGAGCTGAAC+AGG - chr1.1:26569427-26569446 None:intergenic 55.0%
ACCAGCATGACTTCTGGGTC+AGG + chr1.1:26569184-26569203 MS.gene65930:intron 55.0%
ACCTGACCCAGAAGTCATGC+TGG - chr1.1:26569188-26569207 None:intergenic 55.0%
CACCCGCTAACTTAGGGTCA+TGG - chr1.1:26568867-26568886 None:intergenic 55.0%
CACCTTCAACCTTGCACCTC+TGG - chr1.1:26568492-26568511 None:intergenic 55.0%
! CAGAGGTGCAAGGTTGAAGG+TGG + chr1.1:26568490-26568509 MS.gene65930:intron 55.0%
! TGCCAGAGGTGCAAGGTTGA+AGG + chr1.1:26568487-26568506 MS.gene65930:intron 55.0%
ACTCCTGTTCAGCTCGCGCT+TGG + chr1.1:26569421-26569440 MS.gene65930:CDS 60.0%
TCCTATTCCCCACTAGGCGG+CGG + chr1.1:26568986-26569005 MS.gene65930:CDS 60.0%
! AGAGTGTTCCGCCGCCTAGT+GGG - chr1.1:26568997-26569016 None:intergenic 60.0%
CTAGGCGGCGGAACACTCTC+TGG + chr1.1:26568998-26569017 MS.gene65930:CDS 65.0%
GAGTGTTCCGCCGCCTAGTG+GGG - chr1.1:26568996-26569015 None:intergenic 65.0%
TCCGCCGCCTAGTGGGGAAT+AGG - chr1.1:26568990-26569009 None:intergenic 65.0%
!! GAGAGTGTTCCGCCGCCTAG+TGG - chr1.1:26568998-26569017 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 26568069 26569615 26568069 ID=MS.gene65930
chr1.1 mRNA 26568069 26569615 26568069 ID=MS.gene65930.t1;Parent=MS.gene65930
chr1.1 exon 26568069 26568162 26568069 ID=MS.gene65930.t1.exon1;Parent=MS.gene65930.t1
chr1.1 CDS 26568069 26568162 26568069 ID=cds.MS.gene65930.t1;Parent=MS.gene65930.t1
chr1.1 exon 26568936 26569114 26568936 ID=MS.gene65930.t1.exon2;Parent=MS.gene65930.t1
chr1.1 CDS 26568936 26569114 26568936 ID=cds.MS.gene65930.t1;Parent=MS.gene65930.t1
chr1.1 exon 26569376 26569615 26569376 ID=MS.gene65930.t1.exon3;Parent=MS.gene65930.t1
chr1.1 CDS 26569376 26569615 26569376 ID=cds.MS.gene65930.t1;Parent=MS.gene65930.t1
Gene Sequence

>MS.gene65930

ATGGAATTCGTCCATGCAGCTAAAGTTGAGGGACTTCCAAAGATTGTCAGTATCCAGAACAGCTATAGCTTGCTTGTAAGAAGTCGTTTTGAAGGTAAGTACAGTTCTTTTCTGACGTGGATCAGATCTCATTCATTTATCACTTAATTCTGTATTGCTGGATGTAAAGCTGCAGCTCCTGTTGTCCTTATAGTATATGATTCGTGTTGAATCAAAATGCTACTTTTCGTCGAAAAACGCATTGCACCCCAAGTTTTAAAAGTATTACATTATTGAACCTAGAGAGTCCAAAATAAAAAAGGCTTTCCTGTAGATCTTTGAATACTTGGTCTCTCATGTTAACAGTTATACTATGCTGTATCAATGAAGACTTGTTGTTAAACAAATGATTACAGATAACATGGTTTAGGATTTTAGTTGCCAGAGGTGCAAGGTTGAAGGTGGCAAGAGAAATAGGTGTATTGAGTTTCTTTATCTTACAAGGGTTCTCACAATCAGCAATTTATAGGCATACATTCTCCTGCTTTTGGGGAATTTTTAGCTAAAAGTCCAATTTATCCTTCAAACTTGGGATCATATTCATATGCACTTTGGTCCTTAAACTTCAAAAACGTCCAATGTGATCCGTAAACTTTTAGTGTATGGTTTATTTCTTTTCATCTAATATTCTGTCAAATGTGAACGTGATTGTGTTTTTCGTATGATTCATGTTCAGAGTCCAGTTGAACCCTAACTTGGAGTGGGGTAAATTCATCTTTTTCCATTAAGAAATTTGCAAAAATAGTCACAAGTGCACCATGACCCTAAGTTAGCGGGTGTGAAGTTTTAAGCTTCTTCATGTTCTTAGTTGGTTTGATGTTTTTGCAGTTGATCTCGTGGAAGTTTGCCATCCAAAGAATTGCAACATTGGCTTGCTATCCTATTCCCCACTAGGCGGCGGAACACTCTCTGGAAAATATATAGATATAAATTCTAAAGCTGCAAAAAGTGGAAGACTGAATCTCTTCCCTGGCTACATGGAAAGATATAACCAATCACTTTCACGGGTAAGAAATAATTCTAGTTCTTGTATTTAATGTTTTGACCGTCTTTTTCAAAACCGAATTAGACATCGTACCAGCATGACTTCTGGGTCAGGTTCAATGACTTTAAACCACTCGGACAAAACCGCAATTGAACTGCTCAAATAACTGAACCATCCAGTTCAGTTTGGTTATTATAACACTGGTTCAGACCATACTCTAAACCATGGTTAACAAGATAACCAATTAAGAATTTTCCTTTATTTTTTCATGTTTGGTTTTCAGGAAGCAACTATTAAGTATCTTGAGTTGGCCAAGAAACATGGTTTAACTCCTGTTCAGCTCGCGCTTGGATTTGTGAGAGATCGTCCATTCATGGCGAGTTCAATTATCGGTGCAACATCTGTGGACCAACTAAAAGAAGACATTGATGCTTTTACAACAACTGAACGGCCTTTACCAGCAGAAGTCATGACAGGAATTGAAGCTATCTTCAAGAGATACAAAGATCCTAGCATCCTTTAA

Protein sequence

>MS.gene65930.t1

MEFVHAAKVEGLPKIVSIQNSYSLLVRSRFEVDLVEVCHPKNCNIGLLSYSPLGGGTLSGKYIDINSKAAKSGRLNLFPGYMERYNQSLSREATIKYLELAKKHGLTPVQLALGFVRDRPFMASSIIGATSVDQLKEDIDAFTTTERPLPAEVMTGIEAIFKRYKDPSIL