Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67553.t1 | XP_003612320.1 | 97.5 | 922 | 20 | 1 | 1 | 919 | 1 | 922 | 3.40E-224 | 788.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67553.t1 | Q8S2T0 | 55.6 | 771 | 324 | 6 | 163 | 919 | 22 | 788 | 2.0e-52 | 209.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67553.t1 | G7K011 | 97.5 | 922 | 20 | 1 | 1 | 919 | 1 | 922 | 2.4e-224 | 788.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene67553 | 0.875567 | 2.78E-68 | -1.69E-46 |
| MS.gene049208 | MS.gene67553 | 0.81606 | 6.72E-52 | -1.69E-46 |
| MS.gene049350 | MS.gene67553 | 0.810685 | 1.02E-50 | -1.69E-46 |
| MS.gene049368 | MS.gene67553 | 0.818306 | 2.10E-52 | -1.69E-46 |
| MS.gene049585 | MS.gene67553 | 0.848144 | 7.39E-60 | -1.69E-46 |
| MS.gene049744 | MS.gene67553 | 0.822083 | 2.86E-53 | -1.69E-46 |
| MS.gene049807 | MS.gene67553 | 0.823908 | 1.08E-53 | -1.69E-46 |
| MS.gene050084 | MS.gene67553 | 0.816088 | 6.62E-52 | -1.69E-46 |
| MS.gene050085 | MS.gene67553 | 0.830165 | 3.42E-55 | -1.69E-46 |
| MS.gene050224 | MS.gene67553 | 0.814822 | 1.27E-51 | -1.69E-46 |
| MS.gene050439 | MS.gene67553 | 0.811241 | 7.72E-51 | -1.69E-46 |
| MS.gene050551 | MS.gene67553 | 0.866548 | 2.63E-65 | -1.69E-46 |
| MS.gene050646 | MS.gene67553 | 0.814638 | 1.39E-51 | -1.69E-46 |
| MS.gene050654 | MS.gene67553 | 0.807972 | 3.89E-50 | -1.69E-46 |
| MS.gene050689 | MS.gene67553 | 0.804362 | 2.24E-49 | -1.69E-46 |
| MS.gene051102 | MS.gene67553 | 0.876663 | 1.17E-68 | -1.69E-46 |
| MS.gene051343 | MS.gene67553 | 0.814351 | 1.61E-51 | -1.69E-46 |
| MS.gene051365 | MS.gene67553 | 0.803089 | 4.12E-49 | -1.69E-46 |
| MS.gene051381 | MS.gene67553 | 0.839136 | 1.90E-57 | -1.69E-46 |
| MS.gene051642 | MS.gene67553 | 0.824903 | 6.27E-54 | -1.69E-46 |
| MS.gene051676 | MS.gene67553 | 0.84763 | 1.02E-59 | -1.69E-46 |
| MS.gene051724 | MS.gene67553 | 0.83131 | 1.80E-55 | -1.69E-46 |
| MS.gene051744 | MS.gene67553 | 0.813711 | 2.23E-51 | -1.69E-46 |
| MS.gene051800 | MS.gene67553 | 0.805176 | 1.51E-49 | -1.69E-46 |
| MS.gene051852 | MS.gene67553 | 0.807271 | 5.48E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene67553 | 0.814479 | 1.51E-51 | -1.69E-46 |
| MS.gene052072 | MS.gene67553 | 0.816875 | 4.41E-52 | -1.69E-46 |
| MS.gene052168 | MS.gene67553 | 0.812581 | 3.94E-51 | -1.69E-46 |
| MS.gene052307 | MS.gene67553 | 0.877972 | 4.09E-69 | -1.69E-46 |
| MS.gene052397 | MS.gene67553 | 0.803625 | 3.19E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene67553 | 0.803237 | 3.84E-49 | -1.69E-46 |
| MS.gene052514 | MS.gene67553 | 0.80377 | 2.97E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene67553 | 0.811545 | 6.64E-51 | -1.69E-46 |
| MS.gene052609 | MS.gene67553 | 0.908916 | 1.01E-81 | -1.69E-46 |
| MS.gene052776 | MS.gene67553 | 0.80673 | 7.14E-50 | -1.69E-46 |
| MS.gene052932 | MS.gene67553 | 0.844634 | 6.69E-59 | -1.69E-46 |
| MS.gene053079 | MS.gene67553 | 0.818451 | 1.95E-52 | -1.69E-46 |
| MS.gene053143 | MS.gene67553 | 0.831245 | 1.86E-55 | -1.69E-46 |
| MS.gene05315 | MS.gene67553 | 0.813287 | 2.76E-51 | -1.69E-46 |
| MS.gene05350 | MS.gene67553 | 0.818202 | 2.22E-52 | -1.69E-46 |
| MS.gene05462 | MS.gene67553 | 0.808767 | 2.63E-50 | -1.69E-46 |
| MS.gene054824 | MS.gene67553 | 0.820165 | 7.92E-53 | -1.69E-46 |
| MS.gene055310 | MS.gene67553 | 0.803593 | 3.24E-49 | -1.69E-46 |
| MS.gene055311 | MS.gene67553 | 0.848366 | 6.42E-60 | -1.69E-46 |
| MS.gene055313 | MS.gene67553 | 0.865785 | 4.59E-65 | -1.69E-46 |
| MS.gene055314 | MS.gene67553 | 0.847693 | 9.84E-60 | -1.69E-46 |
| MS.gene055465 | MS.gene67553 | 0.833005 | 6.84E-56 | -1.69E-46 |
| MS.gene055469 | MS.gene67553 | 0.844275 | 8.36E-59 | -1.69E-46 |
| MS.gene055513 | MS.gene67553 | 0.847115 | 1.42E-59 | -1.69E-46 |
| MS.gene055864 | MS.gene67553 | 0.849869 | 2.45E-60 | -1.69E-46 |
| MS.gene056045 | MS.gene67553 | 0.82056 | 6.43E-53 | -1.69E-46 |
| MS.gene056181 | MS.gene67553 | 0.819629 | 1.05E-52 | -1.69E-46 |
| MS.gene056308 | MS.gene67553 | 0.833966 | 3.94E-56 | -1.69E-46 |
| MS.gene056390 | MS.gene67553 | 0.80888 | 2.49E-50 | -1.69E-46 |
| MS.gene056507 | MS.gene67553 | 0.848169 | 7.28E-60 | -1.69E-46 |
| MS.gene057360 | MS.gene67553 | 0.866218 | 3.35E-65 | -1.69E-46 |
| MS.gene057915 | MS.gene67553 | 0.824671 | 7.11E-54 | -1.69E-46 |
| MS.gene058010 | MS.gene67553 | 0.802522 | 5.39E-49 | -1.69E-46 |
| MS.gene058081 | MS.gene67553 | 0.827063 | 1.92E-54 | -1.69E-46 |
| MS.gene058289 | MS.gene67553 | 0.826677 | 2.38E-54 | -1.69E-46 |
| MS.gene058292 | MS.gene67553 | 0.851897 | 6.58E-61 | -1.69E-46 |
| MS.gene058638 | MS.gene67553 | 0.829481 | 5.03E-55 | -1.69E-46 |
| MS.gene058639 | MS.gene67553 | 0.83682 | 7.48E-57 | -1.69E-46 |
| MS.gene058647 | MS.gene67553 | 0.813409 | 2.60E-51 | -1.69E-46 |
| MS.gene058649 | MS.gene67553 | 0.80462 | 1.98E-49 | -1.69E-46 |
| MS.gene058852 | MS.gene67553 | 0.807752 | 4.34E-50 | -1.69E-46 |
| MS.gene058919 | MS.gene67553 | 0.852135 | 5.63E-61 | -1.69E-46 |
| MS.gene05897 | MS.gene67553 | 0.823881 | 1.09E-53 | -1.69E-46 |
| MS.gene059007 | MS.gene67553 | 0.812092 | 5.05E-51 | -1.69E-46 |
| MS.gene059144 | MS.gene67553 | 0.804777 | 1.84E-49 | -1.69E-46 |
| MS.gene059171 | MS.gene67553 | 0.844986 | 5.38E-59 | -1.69E-46 |
| MS.gene059439 | MS.gene67553 | 0.828534 | 8.52E-55 | -1.69E-46 |
| MS.gene059713 | MS.gene67553 | 0.822929 | 1.82E-53 | -1.69E-46 |
| MS.gene059725 | MS.gene67553 | 0.820844 | 5.53E-53 | -1.69E-46 |
| MS.gene059965 | MS.gene67553 | 0.818863 | 1.57E-52 | -1.69E-46 |
| MS.gene06010 | MS.gene67553 | 0.802092 | 6.61E-49 | -1.69E-46 |
| MS.gene060191 | MS.gene67553 | 0.809081 | 2.26E-50 | -1.69E-46 |
| MS.gene060380 | MS.gene67553 | 0.833987 | 3.89E-56 | -1.69E-46 |
| MS.gene060400 | MS.gene67553 | 0.811527 | 6.70E-51 | -1.69E-46 |
| MS.gene060637 | MS.gene67553 | 0.811852 | 5.69E-51 | -1.69E-46 |
| MS.gene060785 | MS.gene67553 | 0.800528 | 1.38E-48 | -1.69E-46 |
| MS.gene060933 | MS.gene67553 | 0.839166 | 1.86E-57 | -1.69E-46 |
| MS.gene060935 | MS.gene67553 | 0.805591 | 1.24E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene67553 | 0.823331 | 1.47E-53 | -1.69E-46 |
| MS.gene061006 | MS.gene67553 | 0.83976 | 1.31E-57 | -1.69E-46 |
| MS.gene061100 | MS.gene67553 | 0.833773 | 4.40E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene67553 | MS.gene041802 | PPI |
| MS.gene069114 | MS.gene67553 | PPI |
| MS.gene67553 | MS.gene00013 | PPI |
| MS.gene67553 | MS.gene02723 | PPI |
| MS.gene67553 | MS.gene02153 | PPI |
| MS.gene67553 | MS.gene55989 | PPI |
| MS.gene67553 | MS.gene047988 | PPI |
| MS.gene67553 | MS.gene00155 | PPI |
| MS.gene058237 | MS.gene67553 | PPI |
| MS.gene67553 | MS.gene070689 | PPI |
| MS.gene67553 | MS.gene008939 | PPI |
| MS.gene67553 | MS.gene070223 | PPI |
| MS.gene67553 | MS.gene061266 | PPI |
| MS.gene67553 | MS.gene61712 | PPI |
| MS.gene67553 | MS.gene027794 | PPI |
| MS.gene67553 | MS.gene046436 | PPI |
| MS.gene67553 | MS.gene065220 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67553.t1 | MTR_5g023770 | 97.072 | 922 | 24 | 1 | 1 | 919 | 1 | 922 | 0.0 | 1784 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67553.t1 | AT5G66030 | 63.943 | 771 | 260 | 7 | 163 | 919 | 22 | 788 | 0.0 | 858 |
| MS.gene67553.t1 | AT5G66030 | 62.663 | 766 | 255 | 6 | 163 | 919 | 22 | 765 | 0.0 | 830 |
| MS.gene67553.t1 | AT5G66030 | 63.521 | 710 | 248 | 5 | 163 | 863 | 22 | 729 | 0.0 | 774 |
| MS.gene67553.t1 | AT5G66030 | 61.702 | 658 | 241 | 5 | 163 | 811 | 22 | 677 | 0.0 | 701 |
Find 179 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGATTCTCATTTACTTAA+TGG | 0.104508 | 5.4:+15259160 | MS.gene67553:CDS |
| TTAATATGTACTTCTTATTT+TGG | 0.160688 | 5.4:-15254131 | None:intergenic |
| CACTTTCATCCTTCCTTTGC+TGG | 0.174319 | 5.4:-15248716 | None:intergenic |
| AGCTTCTTCGGATGCAATTC+TGG | 0.218013 | 5.4:-15249324 | None:intergenic |
| GTAAATATTGTATTGGAAAA+TGG | 0.236129 | 5.4:+15248741 | MS.gene67553:CDS |
| TGCTAGCTCTTCCTCCCTTT+GGG | 0.241742 | 5.4:-15257656 | None:intergenic |
| ACCAACTTCTTTCTGTATAC+TGG | 0.244185 | 5.4:-15249213 | None:intergenic |
| ATGTACAGCCTTGCATAAAT+TGG | 0.254936 | 5.4:+15257234 | MS.gene67553:intron |
| TTTGCAGAATTTGGAGAGTT+TGG | 0.258175 | 5.4:+15252245 | MS.gene67553:intron |
| AGAATTCATTCTGAGATTTC+AGG | 0.260515 | 5.4:-15249148 | None:intergenic |
| GAAGTATTGCTACCAGTTAT+TGG | 0.264528 | 5.4:+15258452 | MS.gene67553:CDS |
| GTGCTAGCTCTTCCTCCCTT+TGG | 0.277076 | 5.4:-15257657 | None:intergenic |
| CCATCTGACAAATGCTCCTC+TGG | 0.321478 | 5.4:-15248921 | None:intergenic |
| AAGCTGGGAGGCTGCATCTC+TGG | 0.322039 | 5.4:+15254048 | MS.gene67553:CDS |
| TGCAGTTGCTCTCGGCAATT+TGG | 0.322122 | 5.4:-15254087 | None:intergenic |
| GACTCAAGAGCCTCCTCTTT+AGG | 0.322229 | 5.4:-15251537 | None:intergenic |
| ATTAGTTAGAACCTCTTCTT+TGG | 0.331826 | 5.4:-15254286 | None:intergenic |
| GAGATGGAGGCAGCATCAAC+TGG | 0.349106 | 5.4:+15252974 | MS.gene67553:CDS |
| GATCCTGAAGAGTCGCTTGC+AGG | 0.356621 | 5.4:-15259120 | None:intergenic |
| CATTGCTAAACCCCTCAATC+TGG | 0.360562 | 5.4:-15249178 | None:intergenic |
| GAGATACCATTGAGGATTTA+CGG | 0.361062 | 5.4:+15251502 | MS.gene67553:CDS |
| CTCTGATGGAAATGAAGGTT+TGG | 0.379222 | 5.4:+15249088 | MS.gene67553:CDS |
| CTCTCTGAGCAGCTTGTAGA+AGG | 0.379695 | 5.4:+15249398 | MS.gene67553:CDS |
| AAGTATTGCTACCAGTTATT+GGG | 0.380552 | 5.4:+15258453 | MS.gene67553:CDS |
| ATTCACCTGCAGTGAAGAAA+TGG | 0.390871 | 5.4:-15252316 | None:intergenic |
| GCAATTGTCACTTGCAGAAA+AGG | 0.391238 | 5.4:+15251563 | MS.gene67553:CDS |
| GTCAGATGGAAACGAAGTTC+TGG | 0.395150 | 5.4:+15248935 | MS.gene67553:CDS |
| AACTTGATGCCAAGTTGAAT+AGG | 0.404833 | 5.4:+15249593 | MS.gene67553:CDS |
| AACGAAGGTCCGACTGATAC+TGG | 0.405806 | 5.4:+15248861 | MS.gene67553:CDS |
| AGAAGATATGCAGAAGGAAT+TGG | 0.406447 | 5.4:+15256644 | MS.gene67553:CDS |
| AAGGTGCTTCAAAGCTTCTT+CGG | 0.415250 | 5.4:-15249336 | None:intergenic |
| CCATCTGACAAATGCTCCTC+GGG | 0.415545 | 5.4:-15248837 | None:intergenic |
| TGAAGCAGCATCCCAATAAC+TGG | 0.416643 | 5.4:-15258464 | None:intergenic |
| GCATCTGGTGAAGGTGATAC+TGG | 0.418417 | 5.4:+15248624 | MS.gene67553:CDS |
| ATCTTAAAGCTTCTTGAGAC+AGG | 0.425588 | 5.4:+15258307 | MS.gene67553:CDS |
| CCTAAAAGAGAAAGAGGAAT+GGG | 0.431476 | 5.4:+15256456 | MS.gene67553:CDS |
| CATCTGAGTACGCAATTTGA+AGG | 0.437273 | 5.4:-15249355 | None:intergenic |
| AACAAGAAGTCGATGGCATC+TGG | 0.438022 | 5.4:+15248609 | MS.gene67553:CDS |
| AGTTGAATAGGCTTCACAAA+AGG | 0.438247 | 5.4:+15249605 | MS.gene67553:CDS |
| AGTCCTGCAAGCGACTCTTC+AGG | 0.438561 | 5.4:+15259117 | MS.gene67553:CDS |
| CGATTCTCTGATGGAAATGA+AGG | 0.440056 | 5.4:+15249083 | MS.gene67553:CDS |
| ATCTCTCATACTGAGGATCT+TGG | 0.441672 | 5.4:-15254247 | None:intergenic |
| TCCATCTGACAAATGCTCCT+CGG | 0.447011 | 5.4:-15248838 | None:intergenic |
| AACGAAGTTCTGGCTGATAC+TGG | 0.448934 | 5.4:+15248945 | MS.gene67553:CDS |
| GAGAATCGATTATCTAATAC+AGG | 0.455565 | 5.4:-15249068 | None:intergenic |
| AATGAAGGTCCGGCTGATAC+TGG | 0.456678 | 5.4:+15248777 | MS.gene67553:CDS |
| GGAGGTTTCTCACTTAAAAC+AGG | 0.457424 | 5.4:+15253039 | MS.gene67553:CDS |
| TGAGTCCATTTCTTCACTGC+AGG | 0.458180 | 5.4:+15252311 | MS.gene67553:CDS |
| AAGTTGGTGGTACGGAAGAC+GGG | 0.460944 | 5.4:+15249228 | MS.gene67553:CDS |
| AAGCTGCTCAGAGAGATCTT+TGG | 0.462974 | 5.4:-15249390 | None:intergenic |
| ACATGGAAAGATCAAAGAAA+AGG | 0.464816 | 5.4:+15258251 | MS.gene67553:CDS |
| TGAAAGCCATGGATGGTGAT+AGG | 0.465393 | 5.4:+15250192 | MS.gene67553:CDS |
| TCTTTCCATGTTGCGAAGCT+CGG | 0.465394 | 5.4:-15258239 | None:intergenic |
| ATGAATCAGCAAAGCAAAGA+TGG | 0.471482 | 5.4:+15249032 | MS.gene67553:CDS |
| GTCACTTGCAGAAAAGGAAC+AGG | 0.473473 | 5.4:+15251569 | MS.gene67553:CDS |
| GCAAGCGACTCTTCAGGATC+TGG | 0.473959 | 5.4:+15259123 | MS.gene67553:CDS |
| TCACGATGAGCTTCTGCAAA+TGG | 0.473971 | 5.4:+15249118 | MS.gene67553:CDS |
| ATCTGATGGAAATGAAGGTC+CGG | 0.480640 | 5.4:+15248767 | MS.gene67553:CDS |
| AGATGCCGAACATCAAATTC+TGG | 0.481340 | 5.4:+15257279 | MS.gene67553:CDS |
| GAATCAGACAAATGCTCCTC+AGG | 0.483026 | 5.4:-15249005 | None:intergenic |
| GCTCTCTGCTAAACATCAAA+AGG | 0.484789 | 5.4:+15252036 | MS.gene67553:CDS |
| CATGGAAAGATCAAAGAAAA+GGG | 0.485019 | 5.4:+15258252 | MS.gene67553:CDS |
| TAGTTGCAATGCTGACTGTT+GGG | 0.488336 | 5.4:-15250132 | None:intergenic |
| CTATACCAGAATTTGATGTT+CGG | 0.489165 | 5.4:-15257284 | None:intergenic |
| GGCTAGACAACAAGCCCAAA+GGG | 0.489632 | 5.4:+15257642 | MS.gene67553:CDS |
| GCAGAATTTGGAGAGTTTGG+AGG | 0.490026 | 5.4:+15252248 | MS.gene67553:intron |
| TTCTTGAAATCCCAGATTGA+GGG | 0.490247 | 5.4:+15249167 | MS.gene67553:CDS |
| TTCTTTCTCGTTATTAGCCA+TGG | 0.491838 | 5.4:-15256210 | None:intergenic |
| GGCTTATCTGATGGAAATGA+AGG | 0.492082 | 5.4:+15248762 | MS.gene67553:CDS |
| GAAGAGTCGCTTGCAGGACT+CGG | 0.492796 | 5.4:-15259114 | None:intergenic |
| GTTATCGATAACAGAAGAAA+GGG | 0.494196 | 5.4:+15256160 | MS.gene67553:CDS |
| GTGTGTAGCTCAAAATAAGC+TGG | 0.495154 | 5.4:+15249534 | MS.gene67553:intron |
| CTCAGCTGCCTTTCTCCTAT+CGG | 0.496439 | 5.4:-15252292 | None:intergenic |
| CTCAGCAGATTCGTTTAACT+CGG | 0.497634 | 5.4:-15250099 | None:intergenic |
| AGGTGATACTGGTGGAAAGA+TGG | 0.501992 | 5.4:+15248635 | MS.gene67553:CDS |
| TGTTATCGATAACAGAAGAA+AGG | 0.505115 | 5.4:+15256159 | MS.gene67553:CDS |
| TCAAAAGAAGGACGCAGACT+TGG | 0.505650 | 5.4:+15255988 | MS.gene67553:CDS |
| AAATCGTCTCCACAGCCAAC+AGG | 0.508271 | 5.4:+15257826 | MS.gene67553:CDS |
| TTAGATAATCGATTCTCTGA+TGG | 0.510758 | 5.4:+15249074 | MS.gene67553:CDS |
| AGAATCTAGAATTGCAGAGA+TGG | 0.511223 | 5.4:+15252958 | MS.gene67553:CDS |
| GTTGAATAGGCTTCACAAAA+GGG | 0.513691 | 5.4:+15249606 | MS.gene67553:CDS |
| ACGCATTCAAGAAGTTCAAA+AGG | 0.518457 | 5.4:+15249636 | MS.gene67553:CDS |
| TCAACACCTAAAAGAGAAAG+AGG | 0.519235 | 5.4:+15256450 | MS.gene67553:CDS |
| TCATATCAGAATCAGACAAA+TGG | 0.519407 | 5.4:-15248692 | None:intergenic |
| GCTAATTCAGGAAGAAATTG+AGG | 0.520210 | 5.4:+15257790 | MS.gene67553:intron |
| CCAGAGGAGCATTTGTCAGA+TGG | 0.521431 | 5.4:+15248921 | MS.gene67553:CDS |
| AAGTGATGTAAATATTGTAT+TGG | 0.523227 | 5.4:+15248734 | MS.gene67553:CDS |
| ATTCTTGAAATCCCAGATTG+AGG | 0.523848 | 5.4:+15249166 | MS.gene67553:CDS |
| TTGTTGCCTATCACCATCCA+TGG | 0.524711 | 5.4:-15250198 | None:intergenic |
| TTTGTGAAGCCTATTCAACT+TGG | 0.524900 | 5.4:-15249602 | None:intergenic |
| GCGTTCATTGCAACCTAAAG+AGG | 0.525275 | 5.4:+15251524 | MS.gene67553:CDS |
| GAAGTTGGTGGTACGGAAGA+CGG | 0.526029 | 5.4:+15249227 | MS.gene67553:CDS |
| CCCGAGGAGCATTTGTCAGA+TGG | 0.527662 | 5.4:+15248837 | MS.gene67553:CDS |
| TTGTGAAAGAACTTCGAGAA+AGG | 0.527882 | 5.4:+15249261 | MS.gene67553:CDS |
| AATTTGATGTTCGGCATCTG+AGG | 0.535891 | 5.4:-15257275 | None:intergenic |
| GGGGTTTAGCAATGTTGAGA+GGG | 0.537454 | 5.4:+15249187 | MS.gene67553:CDS |
| TTGGAAAATGGCTTATCTGA+TGG | 0.538678 | 5.4:+15248753 | MS.gene67553:CDS |
| AACAAGGAAACAAGCAAATG+AGG | 0.539583 | 5.4:+15250166 | MS.gene67553:CDS |
| ATTTCTTTGATTAGGTGAAG+TGG | 0.540575 | 5.4:+15258430 | MS.gene67553:intron |
| ATGGCCAGGTCAGCTTGAAT+CGG | 0.541503 | 5.4:+15254209 | MS.gene67553:intron |
| TCCAGTATACAGAAAGAAGT+TGG | 0.542727 | 5.4:+15249212 | MS.gene67553:CDS |
| TGCACGTATGCAGTTGCTCT+CGG | 0.543333 | 5.4:-15254095 | None:intergenic |
| AAAATGTCAAGCAACAGATA+AGG | 0.544513 | 5.4:+15256395 | MS.gene67553:CDS |
| AGCACGGCTCAGAGCAGCCA+TGG | 0.546795 | 5.4:+15253003 | MS.gene67553:CDS |
| TGCTGTATCCGATAGGAGAA+AGG | 0.548396 | 5.4:+15252284 | MS.gene67553:CDS |
| CATCAACTGGGGAAGCAGCA+CGG | 0.549796 | 5.4:+15252987 | MS.gene67553:CDS |
| AGGGGTTTAGCAATGTTGAG+AGG | 0.551883 | 5.4:+15249186 | MS.gene67553:CDS |
| ATTGCTAAACCCCTCAATCT+GGG | 0.551982 | 5.4:-15249177 | None:intergenic |
| AGACATATACCTGTTGGCTG+TGG | 0.558875 | 5.4:-15257835 | None:intergenic |
| GTGGTCTTGATTGAAGAGAC+TGG | 0.561811 | 5.4:-15256899 | None:intergenic |
| CTAGTTGCAATGCTGACTGT+TGG | 0.563335 | 5.4:-15250133 | None:intergenic |
| AACTAGAGTTAGAGCGAACA+AGG | 0.566053 | 5.4:+15250150 | MS.gene67553:CDS |
| AGCAGCCATGGAAAGTGTGA+AGG | 0.566751 | 5.4:+15253015 | MS.gene67553:CDS |
| ACAGAAAGAAGTTGGTGGTA+CGG | 0.566778 | 5.4:+15249220 | MS.gene67553:CDS |
| TCAAGATCTTCAATCTGCCA+TGG | 0.567592 | 5.4:+15256193 | MS.gene67553:CDS |
| AGTTGAATCCAATTTATGCA+AGG | 0.567595 | 5.4:-15257242 | None:intergenic |
| TGAACGCCGTAAATCCTCAA+TGG | 0.567971 | 5.4:-15251508 | None:intergenic |
| ACCTAAAAGAGAAAGAGGAA+TGG | 0.568959 | 5.4:+15256455 | MS.gene67553:CDS |
| AGATGGAGGCAGCATCAACT+GGG | 0.573919 | 5.4:+15252975 | MS.gene67553:CDS |
| ATTTGATGTTCGGCATCTGA+GGG | 0.574149 | 5.4:-15257274 | None:intergenic |
| ACATGCACTTCTTCAAAAGA+AGG | 0.575291 | 5.4:+15255976 | MS.gene67553:CDS |
| TATGAGAGATGCTGAACTGT+TGG | 0.575729 | 5.4:+15254260 | MS.gene67553:CDS |
| TGCAGATCAGCAGTCTTGAG+AGG | 0.575989 | 5.4:+15255927 | MS.gene67553:intron |
| ACGAGAAAGAACTTGCAGAA+AGG | 0.576540 | 5.4:+15256222 | MS.gene67553:CDS |
| ATCTAGAATTGCAGAGATGG+AGG | 0.576609 | 5.4:+15252961 | MS.gene67553:CDS |
| AAAGTCCGAGCTTCGCAACA+TGG | 0.577308 | 5.4:+15258234 | MS.gene67553:CDS |
| GCAGCCATGGAAAGTGTGAA+GGG | 0.580624 | 5.4:+15253016 | MS.gene67553:CDS |
| AAGTCGATGGCATCTGGTGA+AGG | 0.582381 | 5.4:+15248615 | MS.gene67553:CDS |
| TTCAAAATGTAGAAGAAACA+TGG | 0.582714 | 5.4:+15256488 | MS.gene67553:CDS |
| TCTATCAGCAAGATCATGAA+AGG | 0.585507 | 5.4:-15256817 | None:intergenic |
| GACTGATACTGGTGGAACGA+TGG | 0.588028 | 5.4:+15248872 | MS.gene67553:CDS |
| GAAAGATCAAAGAAAAGGGA+AGG | 0.590834 | 5.4:+15258256 | MS.gene67553:CDS |
| TCTTGAAATCCCAGATTGAG+GGG | 0.593814 | 5.4:+15249168 | MS.gene67553:CDS |
| GCAGATCAGCAGTCTTGAGA+GGG | 0.593969 | 5.4:+15255928 | MS.gene67553:intron |
| GGCTGATACTGGTGGAACGA+TGG | 0.598449 | 5.4:+15248956 | MS.gene67553:CDS |
| TGGCTAGACAACAAGCCCAA+AGG | 0.600900 | 5.4:+15257641 | MS.gene67553:CDS |
| TGATATGAACCAGCAAAGGA+AGG | 0.603160 | 5.4:+15248707 | MS.gene67553:CDS |
| GAGTCGCTTGCAGGACTCGG+TGG | 0.603972 | 5.4:-15259111 | None:intergenic |
| GCCAGGTCAGCTTGAATCGG+AGG | 0.605175 | 5.4:+15254212 | MS.gene67553:intron |
| CAAAATGATAATTATAACAC+AGG | 0.605324 | 5.4:+15257056 | MS.gene67553:CDS |
| ACAGTTCCACCAGTATCAGC+CGG | 0.609423 | 5.4:-15248786 | None:intergenic |
| AAATGAGGCGTTGAAAGCCA+TGG | 0.618853 | 5.4:+15250181 | MS.gene67553:CDS |
| AGTCGCTTGCAGGACTCGGT+GGG | 0.620079 | 5.4:-15259110 | None:intergenic |
| GTTCAGCATCTCTCATACTG+AGG | 0.620292 | 5.4:-15254254 | None:intergenic |
| ATTCTGATATGAACCAGCAA+AGG | 0.620546 | 5.4:+15248703 | MS.gene67553:CDS |
| GAAGTTCTGGCTGATACTGG+TGG | 0.621730 | 5.4:+15248948 | MS.gene67553:CDS |
| ACTGTTGGCTGCCAAAGAAG+AGG | 0.621928 | 5.4:+15254275 | MS.gene67553:CDS |
| TTCATTGCAACCTAAAGAGG+AGG | 0.621987 | 5.4:+15251527 | MS.gene67553:CDS |
| GAAGGTCCGGCTGATACTGG+TGG | 0.623619 | 5.4:+15248780 | MS.gene67553:CDS |
| GGCTGATACTGGTGGAACTG+TGG | 0.625396 | 5.4:+15248788 | MS.gene67553:CDS |
| GCTGCTTCAGTTTAGTCCAG+AGG | 0.627685 | 5.4:+15258478 | MS.gene67553:CDS |
| ATCGTTCCACCAGTATCAGT+CGG | 0.628423 | 5.4:-15248870 | None:intergenic |
| TCTTGCTGATAGAATGATTG+AGG | 0.629183 | 5.4:+15256827 | MS.gene67553:CDS |
| CAGCCATGGAAAGTGTGAAG+GGG | 0.629398 | 5.4:+15253017 | MS.gene67553:CDS |
| AGGATTGCTTCGAACTGCCG+AGG | 0.631572 | 5.4:+15251988 | MS.gene67553:CDS |
| GTACTCAGATGCAGAAGCAA+AGG | 0.633740 | 5.4:+15249367 | MS.gene67553:CDS |
| TGCTGCAGAAGATATGCAGA+AGG | 0.633937 | 5.4:+15256638 | MS.gene67553:CDS |
| AGAATCTTGAGAAAAGAGAG+AGG | 0.634025 | 5.4:-15259146 | None:intergenic |
| TCAATGATGCTGTATCCGAT+AGG | 0.635857 | 5.4:+15252277 | MS.gene67553:CDS |
| TGAACAACTCAAAGCTCTTG+AGG | 0.642235 | 5.4:+15256027 | MS.gene67553:CDS |
| GCTTCAGTTTAGTCCAGAGG+AGG | 0.643537 | 5.4:+15258481 | MS.gene67553:CDS |
| CAGACTTCGAGATACCATTG+AGG | 0.644254 | 5.4:+15251494 | MS.gene67553:intron |
| AGCCATGGAAAGTGTGAAGG+GGG | 0.645092 | 5.4:+15253018 | MS.gene67553:CDS |
| AGTTGGTGGTACGGAAGACG+GGG | 0.650080 | 5.4:+15249229 | MS.gene67553:CDS |
| AGTATACAGAAAGAAGTTGG+TGG | 0.653400 | 5.4:+15249215 | MS.gene67553:CDS |
| TCTGGTGAAGGTGATACTGG+TGG | 0.663250 | 5.4:+15248627 | MS.gene67553:CDS |
| GAGGCGTTGAAAGCCATGGA+TGG | 0.667270 | 5.4:+15250185 | MS.gene67553:CDS |
| CATTTGTCAGATGGAAACGA+AGG | 0.675140 | 5.4:+15248846 | MS.gene67553:CDS |
| ACAAGATTCGTCTAAACCCG+AGG | 0.677394 | 5.4:+15248821 | MS.gene67553:CDS |
| GAAGGTCCGACTGATACTGG+TGG | 0.678137 | 5.4:+15248864 | MS.gene67553:CDS |
| ACAACTCAAAGCTCTTGAGG+AGG | 0.681638 | 5.4:+15256030 | MS.gene67553:CDS |
| TAGACAACAAGCCCAAAGGG+AGG | 0.685887 | 5.4:+15257645 | MS.gene67553:CDS |
| GAACTTGGCAGTCAGATGCG+AGG | 0.690916 | 5.4:+15256590 | MS.gene67553:intron |
| ACAAGATTCGTCAAAACCTG+AGG | 0.691768 | 5.4:+15248989 | MS.gene67553:CDS |
| ACAAGATTCATGTAAACCAG+AGG | 0.708170 | 5.4:+15248905 | MS.gene67553:CDS |
| CAGATGCTGGAAGATATGAG+AGG | 0.711762 | 5.4:+15251968 | MS.gene67553:intron |
| CTAAAAGAGAAAGAGGAATG+GGG | 0.715540 | 5.4:+15256457 | MS.gene67553:CDS |
| GATGGAGGCAGCATCAACTG+GGG | 0.716627 | 5.4:+15252976 | MS.gene67553:CDS |
| AAAAGAAAGGGAAAGCTGGG+AGG | 0.732734 | 5.4:+15254036 | MS.gene67553:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 15248591 | 15259179 | 15248591 | ID=MS.gene67553 |
| chr5.4 | mRNA | 15248591 | 15259179 | 15248591 | ID=MS.gene67553.t1;Parent=MS.gene67553 |
| chr5.4 | exon | 15248591 | 15249419 | 15248591 | ID=MS.gene67553.t1.exon1;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15248591 | 15249419 | 15248591 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15249542 | 15249657 | 15249542 | ID=MS.gene67553.t1.exon2;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15249542 | 15249657 | 15249542 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15250071 | 15250240 | 15250071 | ID=MS.gene67553.t1.exon3;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15250071 | 15250240 | 15250071 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15251497 | 15251590 | 15251497 | ID=MS.gene67553.t1.exon4;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15251497 | 15251590 | 15251497 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15251971 | 15252057 | 15251971 | ID=MS.gene67553.t1.exon5;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15251971 | 15252057 | 15251971 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15252252 | 15252332 | 15252252 | ID=MS.gene67553.t1.exon6;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15252252 | 15252332 | 15252252 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15252941 | 15253066 | 15252941 | ID=MS.gene67553.t1.exon7;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15252941 | 15253066 | 15252941 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15254034 | 15254142 | 15254034 | ID=MS.gene67553.t1.exon8;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15254034 | 15254142 | 15254034 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15254217 | 15254296 | 15254217 | ID=MS.gene67553.t1.exon9;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15254217 | 15254296 | 15254217 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15255932 | 15256060 | 15255932 | ID=MS.gene67553.t1.exon10;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15255932 | 15256060 | 15255932 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15256143 | 15256243 | 15256143 | ID=MS.gene67553.t1.exon11;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15256143 | 15256243 | 15256143 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15256381 | 15256514 | 15256381 | ID=MS.gene67553.t1.exon12;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15256381 | 15256514 | 15256381 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15256601 | 15256698 | 15256601 | ID=MS.gene67553.t1.exon13;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15256601 | 15256698 | 15256601 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15256804 | 15256923 | 15256804 | ID=MS.gene67553.t1.exon14;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15256804 | 15256923 | 15256804 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15257050 | 15257077 | 15257050 | ID=MS.gene67553.t1.exon15;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15257050 | 15257077 | 15257050 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15257242 | 15257300 | 15257242 | ID=MS.gene67553.t1.exon16;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15257242 | 15257300 | 15257242 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15257637 | 15257708 | 15257637 | ID=MS.gene67553.t1.exon17;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15257637 | 15257708 | 15257637 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15257800 | 15257847 | 15257800 | ID=MS.gene67553.t1.exon18;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15257800 | 15257847 | 15257800 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15258223 | 15258328 | 15258223 | ID=MS.gene67553.t1.exon19;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15258223 | 15258328 | 15258223 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15258444 | 15258502 | 15258444 | ID=MS.gene67553.t1.exon20;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15258444 | 15258502 | 15258444 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| chr5.4 | exon | 15259066 | 15259179 | 15259066 | ID=MS.gene67553.t1.exon21;Parent=MS.gene67553.t1 |
| chr5.4 | CDS | 15259066 | 15259179 | 15259066 | ID=cds.MS.gene67553.t1;Parent=MS.gene67553.t1 |
| Gene Sequence |
| Protein sequence |
