AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73257

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73257.t1 MTR_4g019310 95.954 346 13 1 1 345 155 500 0.0 671
MS.gene73257.t1 MTR_4g017470 41.690 355 179 10 13 344 129 478 5.01e-70 226
MS.gene73257.t1 MTR_4g017830 40.282 355 186 9 8 342 159 507 1.66e-67 221
MS.gene73257.t1 MTR_4g017830 27.092 251 158 8 96 333 1 239 2.23e-14 74.3
MS.gene73257.t1 MTR_4g017550 41.954 348 170 11 6 332 133 469 1.58e-65 214
MS.gene73257.t1 MTR_4g019340 40.785 331 142 9 16 323 202 501 1.65e-62 207
MS.gene73257.t1 MTR_4g019340 28.244 262 160 10 95 333 19 275 3.41e-14 73.9
MS.gene73257.t1 MTR_4g017440 39.820 334 186 8 21 342 178 508 2.61e-61 204
MS.gene73257.t1 MTR_4g017410 38.462 364 171 10 4 345 161 493 1.34e-60 202
MS.gene73257.t1 MTR_4g017410 26.141 241 154 9 112 333 9 244 2.47e-11 65.1
MS.gene73257.t1 MTR_4g017610 38.608 316 169 7 16 323 169 467 8.42e-55 186
MS.gene73257.t1 MTR_7g066570 33.239 352 195 11 12 345 160 489 4.47e-46 165
MS.gene73257.t1 MTR_7g066570 30.802 237 135 11 112 338 14 231 3.85e-15 76.6
MS.gene73257.t1 MTR_7g066260 35.000 360 167 9 19 345 169 494 2.02e-45 162
MS.gene73257.t1 MTR_7g066260 29.915 234 140 13 113 333 16 238 5.71e-13 70.1
MS.gene73257.t1 MTR_7g066350 31.988 322 163 7 12 319 154 433 9.58e-45 159
MS.gene73257.t1 MTR_7g066350 32.035 231 137 7 113 333 10 230 2.54e-17 83.2
MS.gene73257.t1 MTR_4g019330 44.037 218 60 5 126 342 32 188 2.84e-44 150
MS.gene73257.t1 MTR_7g063920 34.835 333 199 8 13 342 119 436 3.10e-44 157
MS.gene73257.t1 MTR_7g062280 33.832 334 196 7 19 342 173 491 4.45e-44 158
MS.gene73257.t1 MTR_7g058710 31.818 352 205 10 10 342 148 483 1.93e-43 156
MS.gene73257.t1 MTR_7g058710 29.004 231 146 9 108 333 8 225 3.02e-16 80.1
MS.gene73257.t1 MTR_7g066400 33.134 335 175 8 16 332 130 433 6.57e-42 151
MS.gene73257.t1 MTR_7g066340 33.538 325 166 7 19 332 164 449 8.15e-42 152
MS.gene73257.t1 MTR_7g066340 30.000 230 145 6 113 333 13 235 8.32e-18 84.7
MS.gene73257.t1 MTR_7g063660 34.718 337 192 11 19 342 156 477 8.85e-42 152
MS.gene73257.t1 MTR_7g066430 34.320 338 202 9 15 337 154 486 1.08e-41 152
MS.gene73257.t1 MTR_7g066580 31.922 307 177 9 49 337 150 442 1.24e-39 145
MS.gene73257.t1 MTR_3g007740 33.634 333 171 10 19 336 145 442 2.12e-39 145
MS.gene73257.t1 MTR_7g063670 34.242 330 182 11 19 342 157 457 2.25e-38 142
MS.gene73257.t1 MTR_7g063890 33.731 335 205 8 19 342 176 504 2.35e-38 143
MS.gene73257.t1 MTR_7g063890 27.234 235 146 11 115 339 30 249 7.41e-12 66.6
MS.gene73257.t1 MTR_7g062300 32.198 323 200 8 19 335 163 472 2.69e-38 142
MS.gene73257.t1 MTR_7g062300 26.531 245 150 11 112 342 12 240 5.56e-11 63.9
MS.gene73257.t1 MTR_1g041315 30.769 325 161 8 19 319 167 451 5.89e-38 142
MS.gene73257.t1 MTR_7g063880 31.065 338 204 10 13 343 154 469 1.82e-37 140
MS.gene73257.t1 MTR_7g063880 29.870 231 133 11 113 330 12 226 8.80e-11 63.2
MS.gene73257.t1 MTR_7g066410 31.667 360 203 11 13 336 160 512 6.21e-36 136
MS.gene73257.t1 MTR_7g063900 34.024 338 201 11 19 342 159 488 1.12e-35 135
MS.gene73257.t1 MTR_7g063840 31.343 335 191 11 24 342 162 473 1.40e-35 135
MS.gene73257.t1 MTR_7g066680 31.955 266 148 6 68 315 37 287 1.75e-35 132
MS.gene73257.t1 MTR_7g012680 33.628 339 169 12 13 342 160 451 1.51e-34 132
MS.gene73257.t1 MTR_7g012680 28.689 244 115 11 113 338 15 217 3.22e-12 67.8
MS.gene73257.t1 MTR_7g063820 30.878 353 225 9 3 342 138 484 2.72e-34 131
MS.gene73257.t1 MTR_7g066360 33.841 328 161 10 16 332 163 445 6.37e-33 127
MS.gene73257.t1 MTR_7g066280 30.861 337 187 11 19 332 73 386 4.20e-32 124
MS.gene73257.t1 MTR_7g066290 32.399 321 150 8 19 332 169 429 8.91e-31 121
MS.gene73257.t1 MTR_3g065200 28.990 307 175 7 19 322 142 408 3.35e-29 117
MS.gene73257.t1 MTR_7g066310 34.199 231 114 6 104 332 185 379 1.00e-28 115
MS.gene73257.t1 MTR_7g063830 31.548 336 178 13 13 342 155 444 4.39e-27 111
MS.gene73257.t1 MTR_7g063720 31.064 235 137 5 109 341 20 231 5.93e-27 107
MS.gene73257.t1 MTR_2g029400 28.438 320 195 8 13 324 111 404 7.72e-27 110
MS.gene73257.t1 MTR_7g066480 31.481 324 145 9 19 332 161 417 5.68e-26 107
MS.gene73257.t1 MTR_7g058700 35.749 207 100 6 10 200 653 842 3.69e-24 103
MS.gene73257.t1 MTR_7g100420 33.948 271 164 9 68 333 134 394 4.48e-23 99.4
MS.gene73257.t1 MTR_7g063850 29.915 234 146 9 115 343 69 289 3.46e-22 95.1
MS.gene73257.t1 MTR_1g014060 33.333 210 106 9 131 332 197 380 2.27e-20 91.7
MS.gene73257.t1 MTR_7g066490 37.791 172 83 5 19 180 160 317 1.66e-19 89.4
MS.gene73257.t1 MTR_7g066490 56.522 69 29 1 264 332 336 403 2.30e-14 73.9
MS.gene73257.t1 MTR_1g115440 27.313 227 149 5 113 329 12 232 4.27e-18 82.8
MS.gene73257.t1 MTR_7g066390 39.823 113 57 4 224 332 13 118 1.06e-14 71.2
MS.gene73257.t1 MTR_8g023240 23.853 327 224 9 16 330 170 483 4.94e-14 73.6
MS.gene73257.t1 MTR_7g063690 34.959 123 78 2 19 139 100 222 5.55e-14 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73257.t1 AT5G01140 33.526 346 202 9 7 342 131 458 4.53e-41 150
MS.gene73257.t1 AT5G01130 31.402 328 180 7 25 343 161 452 6.20e-36 135
MS.gene73257.t1 AT5G01120 31.818 330 207 7 18 335 165 488 1.28e-35 135
MS.gene73257.t1 AT3G09140 33.626 342 201 12 15 343 173 501 1.37e-35 135
MS.gene73257.t1 AT5G01150 31.176 340 212 7 15 343 159 487 2.75e-34 132
MS.gene73257.t1 AT3G09140 31.165 369 200 13 15 342 173 528 4.29e-30 120
MS.gene73257.t1 AT5G43240 31.268 355 205 12 4 335 10 348 3.80e-25 104
MS.gene73257.t1 AT5G43240 31.268 355 205 12 4 335 154 492 1.62e-24 104
MS.gene73257.t1 AT5G43240 31.268 355 205 12 4 335 154 492 1.62e-24 104
MS.gene73257.t1 AT5G43240 31.268 355 205 12 4 335 154 492 1.62e-24 104
MS.gene73257.t1 AT3G09110 39.264 163 95 1 16 174 157 319 4.35e-24 101
MS.gene73257.t1 AT3G09120 40.252 159 88 2 13 166 157 313 1.39e-23 99.4
MS.gene73257.t1 AT3G09120 30.213 235 141 10 109 329 3 228 3.00e-13 70.1
MS.gene73257.t1 AT5G37320 29.023 348 195 15 15 341 168 484 6.02e-20 90.9

Find 64 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAAGGTGGCTCCTCAATT+TGG 0.239522 4.1:+78929218 MS.gene73257:CDS
ATGAAGTTGCATGGAGATTT+TGG 0.248004 4.1:+78927704 MS.gene73257:CDS
TCATGGTTCATAAGGCCTTT+AGG 0.297689 4.1:+78929179 MS.gene73257:CDS
CTTCTTCCTTTGGATTCTTA+AGG 0.299759 4.1:-78930009 None:intergenic
ACCGTCTGTCAAAGCCTCTT+TGG 0.309921 4.1:-78929975 None:intergenic
TGGCACAATTGAATCTGCTT+TGG 0.331646 4.1:+78929418 MS.gene73257:CDS
TGAGGAGCCACCTTTGGATT+TGG 0.337613 4.1:-78929212 None:intergenic
ACACCAATTGGATCTATTCT+AGG 0.341690 4.1:+78929086 MS.gene73257:CDS
GGCACAATTGAATCTGCTTT+GGG 0.350892 4.1:+78929419 MS.gene73257:CDS
CAAGCTGTAACTTCTTCCTT+TGG 0.353157 4.1:-78930019 None:intergenic
GAATGTACCATTGGATGATT+TGG 0.353597 4.1:+78929535 MS.gene73257:CDS
CTCTTTCAATCCAACATTCA+AGG 0.359070 4.1:-78927885 None:intergenic
CCAAATTGAGGAGCCACCTT+TGG 0.362907 4.1:-78929218 None:intergenic
ATTGTGCCATAAGGTGATCT+TGG 0.386765 4.1:-78929404 None:intergenic
TTTGATCCAAGATCACCTTA+TGG 0.391081 4.1:+78929398 MS.gene73257:CDS
TCAATCCTTCATGGTTCATA+AGG 0.394940 4.1:+78929171 MS.gene73257:CDS
ATTTGTTATTCCAATGTGAA+TGG 0.400477 4.1:+78929323 MS.gene73257:CDS
AGTTTCCTCACAACACCAAT+TGG 0.406778 4.1:+78929074 MS.gene73257:CDS
TGAAAGAATTCAATCCTTCA+TGG 0.410331 4.1:+78929162 MS.gene73257:CDS
ATGGACTAATATAAAACTTA+AGG 0.429845 4.1:+78928980 MS.gene73257:CDS
TCTTCACAAAGATTTATTAG+TGG 0.444473 4.1:-78929254 None:intergenic
AGCCACCTTTGGATTTGGTA+AGG 0.450367 4.1:-78929207 None:intergenic
TAATATAAAACTTAAGGTAA+TGG 0.478261 4.1:+78928986 MS.gene73257:CDS
AAGTCAAAGAAGCTCCCAAC+AGG 0.484404 4.1:-78929949 None:intergenic
TTCTTTGACTTCTTCCAAAG+AGG 0.487396 4.1:+78929961 MS.gene73257:CDS
CCACCTTTGGATTTGGTAAG+GGG 0.490596 4.1:-78929205 None:intergenic
CCTACTTATTGGTATGGTAA+TGG 0.492706 4.1:+78929284 MS.gene73257:CDS
GTTCAGCAACTCATTCAACT+TGG 0.493028 4.1:+78927830 MS.gene73257:CDS
GCAGATTCAATTGTGCCATA+AGG 0.493493 4.1:-78929413 None:intergenic
TATATCAGTGAGAGGAGACT+TGG 0.494239 4.1:-78928889 None:intergenic
CTGCTTTGGGATTTGTGAAG+AGG 0.503837 4.1:+78929432 MS.gene73257:CDS
AAAGTGTTGCAAAACTCTCC+TGG 0.503982 4.1:+78927800 MS.gene73257:CDS
TGCTTCATGCAACCAAATTG+AGG 0.504685 4.1:-78929230 None:intergenic
TTTGGTAAGGGGTTACCTAA+AGG 0.505371 4.1:-78929194 None:intergenic
TGTCAGGCACTGAACCTGTT+GGG 0.511727 4.1:+78929935 MS.gene73257:intron
GACAAAGAAATGAAGTTGCA+TGG 0.513467 4.1:+78927695 None:intergenic
TCCAAAGAGGCTTTGACAGA+CGG 0.518614 4.1:+78929974 MS.gene73257:CDS
GAGGAGCATATAGTGAGCAT+TGG 0.518756 4.1:+78929557 MS.gene73257:CDS
GCCACCTTTGGATTTGGTAA+GGG 0.525081 4.1:-78929206 None:intergenic
ACACAAGTGTCCTTGAATGT+TGG 0.529675 4.1:+78927875 MS.gene73257:CDS
CCATTACCATACCAATAAGT+AGG 0.533560 4.1:-78929284 None:intergenic
GGCCATCACTATTTATTGTG+TGG 0.534751 4.1:+78929453 MS.gene73257:CDS
GCCATCACTATTTATTGTGT+GGG 0.535594 4.1:+78929454 MS.gene73257:CDS
AAAGCTCCTACTTATTGGTA+TGG 0.537812 4.1:+78929278 MS.gene73257:CDS
TGTCCTAGAATAGATCCAAT+TGG 0.538385 4.1:-78929089 None:intergenic
CTTCCTTTGGATTCTTAAGG+AGG 0.541667 4.1:-78930006 None:intergenic
GCACAAGATGAATGTACCAT+TGG 0.543115 4.1:+78929526 MS.gene73257:CDS
CTTGAATGTTGGATTGAAAG+AGG 0.547131 4.1:+78927886 MS.gene73257:CDS
TCAACATTCTCGCCAAGACA+TGG 0.561002 4.1:+78928942 MS.gene73257:CDS
TGATATCACTCCATTCACAT+TGG 0.568520 4.1:-78929333 None:intergenic
TGTACCATTGGATGATTTGG+AGG 0.570776 4.1:+78929538 MS.gene73257:CDS
GATACTATATGCTGAAGCTG+AGG 0.584783 4.1:+78929028 MS.gene73257:CDS
AAAGGCCTTATGAACCATGA+AGG 0.592293 4.1:-78929176 None:intergenic
TTGTCAGGCACTGAACCTGT+TGG 0.605048 4.1:+78929934 MS.gene73257:intron
TCCCACACAATAAATAGTGA+TGG 0.607267 4.1:-78929455 None:intergenic
TGCTCCTCCAAATCATCCAA+TGG 0.607505 4.1:-78929542 None:intergenic
ATACTATATGCTGAAGCTGA+GGG 0.609419 4.1:+78929029 MS.gene73257:CDS
CTACCTCCTTAAGAATCCAA+AGG 0.611859 4.1:+78930003 MS.gene73257:CDS
AGTTGCTGAACTGAATTACC+AGG 0.611897 4.1:-78927818 None:intergenic
TAGATCCAATTGGTGTTGTG+AGG 0.624363 4.1:-78929079 None:intergenic
CCCCTTACCAAATCCAAAGG+TGG 0.639710 4.1:+78929205 MS.gene73257:CDS
TAACCCCTTACCAAATCCAA+AGG 0.647813 4.1:+78929202 MS.gene73257:CDS
ATGCTTGAAGTATTTGCGAA+TGG 0.650055 4.1:-78929494 None:intergenic
AGTGAGCATTGGAGAAACAG+AGG 0.672512 4.1:+78929568 MS.gene73257:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATATA+TGG + chr4.1:78929803-78929822 MS.gene73257:intron 0.0%
!!! TAAATTTTTAATATATTTTT+AGG + chr4.1:78927923-78927942 MS.gene73257:intron 0.0%
!! AAAAAATAATAATTTAGACA+TGG + chr4.1:78928669-78928688 MS.gene73257:intron 10.0%
!! AAAATTAATTAAAGAACTTA+AGG - chr4.1:78928741-78928760 None:intergenic 10.0%
!! TATTTATATGGTAATTAAAT+TGG + chr4.1:78928185-78928204 MS.gene73257:intron 10.0%
!!! ATTTTCTTGTTAATTTTTTT+TGG - chr4.1:78929742-78929761 None:intergenic 10.0%
!!! TTTTCTTGTTAATTTTTTTT+GGG - chr4.1:78929741-78929760 None:intergenic 10.0%
!! AAAATTCTAATTTAAATGTG+AGG + chr4.1:78928386-78928405 MS.gene73257:intron 15.0%
!! CATTGATATATTATAAAACT+AGG + chr4.1:78929849-78929868 MS.gene73257:intron 15.0%
!! TAACAAGAAAATAGATATTA+AGG + chr4.1:78929750-78929769 MS.gene73257:intron 15.0%
!! TAATATAAAACTTAAGGTAA+TGG + chr4.1:78928986-78929005 MS.gene73257:CDS 15.0%
!!! AAAATCTAGAATGTTAAAAT+AGG - chr4.1:78928843-78928862 None:intergenic 15.0%
!!! AATTTTTCATGGTATTTATA+TGG + chr4.1:78928173-78928192 MS.gene73257:intron 15.0%
!!! ATTTTTTTTTCTTCCTTAAT+CGG + chr4.1:78929689-78929708 MS.gene73257:intron 15.0%
!!! TAATTTTTAAACATGTGTAT+GGG + chr4.1:78928754-78928773 MS.gene73257:intron 15.0%
!!! TATATTTTTAGGAAAATGTT+TGG + chr4.1:78927934-78927953 MS.gene73257:intron 15.0%
!!! TATTTTAACATTCTAGATTT+TGG + chr4.1:78928842-78928861 MS.gene73257:intron 15.0%
!!! TTAATTTTTAAACATGTGTA+TGG + chr4.1:78928753-78928772 MS.gene73257:intron 15.0%
!!! TTAGTCCATTTTTTAAATTT+TGG - chr4.1:78928969-78928988 None:intergenic 15.0%
!! ATGGACCAAAATTTAAAAAA+TGG + chr4.1:78928961-78928980 MS.gene73257:CDS 20.0%
!! TGATATATTATAAAACTAGG+TGG + chr4.1:78929852-78929871 MS.gene73257:intron 20.0%
!!! AAAACTGATTTTAAAAATCG+TGG + chr4.1:78928426-78928445 MS.gene73257:intron 20.0%
!!! ATGGACTAATATAAAACTTA+AGG + chr4.1:78928980-78928999 MS.gene73257:CDS 20.0%
!!! CATCATTTTAAATTCTTCAT+TGG - chr4.1:78928922-78928941 None:intergenic 20.0%
!!! CTGATATTTTGTAAATTAAC+TGG - chr4.1:78928124-78928143 None:intergenic 20.0%
! AAAATTGACCCTAAATACAT+AGG - chr4.1:78928159-78928178 None:intergenic 25.0%
! ACACATTATATGTAGAAAAG+TGG - chr4.1:78928589-78928608 None:intergenic 25.0%
! CTTGATACTTGGATATTTAA+TGG + chr4.1:78929639-78929658 MS.gene73257:intron 25.0%
! TCTTCACAAAGATTTATTAG+TGG - chr4.1:78929257-78929276 None:intergenic 25.0%
! TTAATTATCTCTACATTGTC+AGG + chr4.1:78929919-78929938 MS.gene73257:intron 25.0%
!! TTGAATGACAATTTTTCTCT+AGG + chr4.1:78929113-78929132 MS.gene73257:CDS 25.0%
!!! ATTTGTTATTCCAATGTGAA+TGG + chr4.1:78929323-78929342 MS.gene73257:CDS 25.0%
!!! GAGAGTTATAATTTTGATGA+TGG + chr4.1:78927731-78927750 MS.gene73257:CDS 25.0%
!!! GATTTTAAAAATCGTGGATT+TGG + chr4.1:78928432-78928451 MS.gene73257:intron 25.0%
!!! GTTTGGCTTATATATAGTTT+TGG + chr4.1:78928040-78928059 MS.gene73257:intron 25.0%
!!! TATTTAGGGTCAATTTTTCA+TGG + chr4.1:78928162-78928181 MS.gene73257:intron 25.0%
AAGAAAAAGCTCCTACTTAT+TGG + chr4.1:78929273-78929292 MS.gene73257:CDS 30.0%
ACGAATTAGACAAAATAGAG+AGG + chr4.1:78928477-78928496 MS.gene73257:intron 30.0%
ATCAATTGAGCTACACTTAT+GGG - chr4.1:78928516-78928535 None:intergenic 30.0%
ATGTAGAAAAGTGGAGAATT+CGG - chr4.1:78928580-78928599 None:intergenic 30.0%
CACGTCATCATTAATTAAGT+TGG + chr4.1:78928334-78928353 MS.gene73257:intron 30.0%
CGAATTAGACAAAATAGAGA+GGG + chr4.1:78928478-78928497 MS.gene73257:intron 30.0%
TATCAATTGAGCTACACTTA+TGG - chr4.1:78928517-78928536 None:intergenic 30.0%
TCAAAAAGTTTCATTGCTTC+TGG - chr4.1:78929383-78929402 None:intergenic 30.0%
TGAAAGAATTCAATCCTTCA+TGG + chr4.1:78929162-78929181 MS.gene73257:CDS 30.0%
TTTCTCCACCTATGTATTTA+GGG + chr4.1:78928148-78928167 MS.gene73257:intron 30.0%
! TCACTTGCTTCTAGTTATAA+AGG - chr4.1:78929894-78929913 None:intergenic 30.0%
! TCCTCTCACTGATATATTTT+TGG + chr4.1:78928896-78928915 MS.gene73257:CDS 30.0%
! TTTTCTCCACCTATGTATTT+AGG + chr4.1:78928147-78928166 MS.gene73257:intron 30.0%
! TTTTGTCTAATTCGTGACAT+TGG - chr4.1:78928472-78928491 None:intergenic 30.0%
!! GATGGTGTTTTTGTTAAGAA+AGG + chr4.1:78927749-78927768 MS.gene73257:CDS 30.0%
!! TTAAATTTTGGTCCATGTCT+TGG - chr4.1:78928957-78928976 None:intergenic 30.0%
!!! AATTTTGATGATGTGACAAG+TGG - chr4.1:78928372-78928391 None:intergenic 30.0%
!!! ATTTTGATGATGTGACAAGT+GGG - chr4.1:78928371-78928390 None:intergenic 30.0%
!!! CTCTATTTTTTTCTCTCTCA+AGG - chr4.1:78927984-78928003 None:intergenic 30.0%
!!! TATTTTTTTCTCTCTCAAGG+CGG - chr4.1:78927981-78928000 None:intergenic 30.0%
AAAGCTCCTACTTATTGGTA+TGG + chr4.1:78929278-78929297 MS.gene73257:CDS 35.0%
ACACCAATTGGATCTATTCT+AGG + chr4.1:78929086-78929105 MS.gene73257:CDS 35.0%
ACTTCTACACGTAAAATCCT+TGG - chr4.1:78928544-78928563 None:intergenic 35.0%
ATACTATATGCTGAAGCTGA+GGG + chr4.1:78929029-78929048 MS.gene73257:CDS 35.0%
ATTGACCCTAAATACATAGG+TGG - chr4.1:78928156-78928175 None:intergenic 35.0%
CCATTACCATACCAATAAGT+AGG - chr4.1:78929287-78929306 None:intergenic 35.0%
CCTACTTATTGGTATGGTAA+TGG + chr4.1:78929284-78929303 MS.gene73257:CDS 35.0%
CTCTTTCAATCCAACATTCA+AGG - chr4.1:78927888-78927907 None:intergenic 35.0%
CTTCTTCCTTTGGATTCTTA+AGG - chr4.1:78930012-78930031 None:intergenic 35.0%
CTTGAATGTTGGATTGAAAG+AGG + chr4.1:78927886-78927905 MS.gene73257:CDS 35.0%
GAATGTACCATTGGATGATT+TGG + chr4.1:78929535-78929554 MS.gene73257:CDS 35.0%
GCCAAAAATATATCAGTGAG+AGG - chr4.1:78928900-78928919 None:intergenic 35.0%
GCCATCACTATTTATTGTGT+GGG + chr4.1:78929454-78929473 MS.gene73257:CDS 35.0%
TCAATCCTTCATGGTTCATA+AGG + chr4.1:78929171-78929190 MS.gene73257:CDS 35.0%
TCCAAGTATCAAGATTTGCA+AGG - chr4.1:78929632-78929651 None:intergenic 35.0%
TCCCACACAATAAATAGTGA+TGG - chr4.1:78929458-78929477 None:intergenic 35.0%
TCCTTGCAAATCTTGATACT+TGG + chr4.1:78929628-78929647 MS.gene73257:intron 35.0%
TGATATCACTCCATTCACAT+TGG - chr4.1:78929336-78929355 None:intergenic 35.0%
TGTAGAAAAGTGGAGAATTC+GGG - chr4.1:78928579-78928598 None:intergenic 35.0%
TGTCCTAGAATAGATCCAAT+TGG - chr4.1:78929092-78929111 None:intergenic 35.0%
TTAAGCTAACTAGTGTCTCA+GGG - chr4.1:78929718-78929737 None:intergenic 35.0%
TTAGACAAAATAGAGAGGGA+TGG + chr4.1:78928482-78928501 MS.gene73257:intron 35.0%
TTTGATCCAAGATCACCTTA+TGG + chr4.1:78929398-78929417 MS.gene73257:CDS 35.0%
! AGCTCAATTGATAGTTACCA+AGG + chr4.1:78928524-78928543 MS.gene73257:intron 35.0%
! AGGATTTTACGTGTAGAAGT+TGG + chr4.1:78928544-78928563 MS.gene73257:intron 35.0%
! ATGAAGTTGCATGGAGATTT+TGG + chr4.1:78927704-78927723 MS.gene73257:CDS 35.0%
! ATGCTTGAAGTATTTGCGAA+TGG - chr4.1:78929497-78929516 None:intergenic 35.0%
! GATGACGTGATATTTCTACA+CGG - chr4.1:78928323-78928342 None:intergenic 35.0%
! TTCTTTGACTTCTTCCAAAG+AGG + chr4.1:78929961-78929980 MS.gene73257:CDS 35.0%
!!! CAATTTTTCTCTAGGATGTG+TGG + chr4.1:78929121-78929140 MS.gene73257:CDS 35.0%
ACACAAGTGTCCTTGAATGT+TGG + chr4.1:78927875-78927894 MS.gene73257:CDS 40.0%
AGTTGCTGAACTGAATTACC+AGG - chr4.1:78927821-78927840 None:intergenic 40.0%
AGTTTCCTCACAACACCAAT+TGG + chr4.1:78929074-78929093 MS.gene73257:CDS 40.0%
ATAGAGAGAGCGAGAATGTT+TGG + chr4.1:78928023-78928042 MS.gene73257:intron 40.0%
ATTGTGCCATAAGGTGATCT+TGG - chr4.1:78929407-78929426 None:intergenic 40.0%
CAAGCTGTAACTTCTTCCTT+TGG - chr4.1:78930022-78930041 None:intergenic 40.0%
CTACCTCCTTAAGAATCCAA+AGG + chr4.1:78930003-78930022 MS.gene73257:CDS 40.0%
CTTCCTTTGGATTCTTAAGG+AGG - chr4.1:78930009-78930028 None:intergenic 40.0%
GATACTATATGCTGAAGCTG+AGG + chr4.1:78929028-78929047 MS.gene73257:CDS 40.0%
GCACAAGATGAATGTACCAT+TGG + chr4.1:78929526-78929545 MS.gene73257:CDS 40.0%
GCAGATTCAATTGTGCCATA+AGG - chr4.1:78929416-78929435 None:intergenic 40.0%
GGCACAATTGAATCTGCTTT+GGG + chr4.1:78929419-78929438 MS.gene73257:CDS 40.0%
GGCCATCACTATTTATTGTG+TGG + chr4.1:78929453-78929472 MS.gene73257:CDS 40.0%
GTAGAAAAGTGGAGAATTCG+GGG - chr4.1:78928578-78928597 None:intergenic 40.0%
GTGGATTTGGAAAAAGCAGA+TGG + chr4.1:78928445-78928464 MS.gene73257:intron 40.0%
GTTAAGCTAACTAGTGTCTC+AGG - chr4.1:78929719-78929738 None:intergenic 40.0%
GTTCAGCAACTCATTCAACT+TGG + chr4.1:78927830-78927849 MS.gene73257:CDS 40.0%
TAACCCCTTACCAAATCCAA+AGG + chr4.1:78929202-78929221 MS.gene73257:CDS 40.0%
TATATCAGTGAGAGGAGACT+TGG - chr4.1:78928892-78928911 None:intergenic 40.0%
TCATGGTTCATAAGGCCTTT+AGG + chr4.1:78929179-78929198 MS.gene73257:CDS 40.0%
TGCTTCATGCAACCAAATTG+AGG - chr4.1:78929233-78929252 None:intergenic 40.0%
TGGCACAATTGAATCTGCTT+TGG + chr4.1:78929418-78929437 MS.gene73257:CDS 40.0%
TTTGGTAAGGGGTTACCTAA+AGG - chr4.1:78929197-78929216 None:intergenic 40.0%
! AAAGGCCTTATGAACCATGA+AGG - chr4.1:78929179-78929198 None:intergenic 40.0%
! TGTACCATTGGATGATTTGG+AGG + chr4.1:78929538-78929557 MS.gene73257:CDS 40.0%
!! AAAGTGTTGCAAAACTCTCC+TGG + chr4.1:78927800-78927819 MS.gene73257:CDS 40.0%
!! GTTTTAGTCTCTCTCTTGGT+TGG - chr4.1:78928638-78928657 None:intergenic 40.0%
!! TAGATCCAATTGGTGTTGTG+AGG - chr4.1:78929082-78929101 None:intergenic 40.0%
!! TGCAGTTTTAGTCTCTCTCT+TGG - chr4.1:78928642-78928661 None:intergenic 40.0%
AAGTCAAAGAAGCTCCCAAC+AGG - chr4.1:78929952-78929971 None:intergenic 45.0%
TCAACATTCTCGCCAAGACA+TGG + chr4.1:78928942-78928961 MS.gene73257:CDS 45.0%
TGCTCCTCCAAATCATCCAA+TGG - chr4.1:78929545-78929564 None:intergenic 45.0%
! AGCCACCTTTGGATTTGGTA+AGG - chr4.1:78929210-78929229 None:intergenic 45.0%
! CCACCTTTGGATTTGGTAAG+GGG - chr4.1:78929208-78929227 None:intergenic 45.0%
! GAGGAGCATATAGTGAGCAT+TGG + chr4.1:78929557-78929576 MS.gene73257:CDS 45.0%
! GCCACCTTTGGATTTGGTAA+GGG - chr4.1:78929209-78929228 None:intergenic 45.0%
! TCCAAAGAGGCTTTGACAGA+CGG + chr4.1:78929974-78929993 MS.gene73257:CDS 45.0%
!! AGTGAGCATTGGAGAAACAG+AGG + chr4.1:78929568-78929587 MS.gene73257:CDS 45.0%
!! CTGCTTTGGGATTTGTGAAG+AGG + chr4.1:78929432-78929451 MS.gene73257:CDS 45.0%
ACCGTCTGTCAAAGCCTCTT+TGG - chr4.1:78929978-78929997 None:intergenic 50.0%
CCAAAGGTGGCTCCTCAATT+TGG + chr4.1:78929218-78929237 MS.gene73257:CDS 50.0%
CCAAATTGAGGAGCCACCTT+TGG - chr4.1:78929221-78929240 None:intergenic 50.0%
CCCCTTACCAAATCCAAAGG+TGG + chr4.1:78929205-78929224 MS.gene73257:CDS 50.0%
TGAGGAGCCACCTTTGGATT+TGG - chr4.1:78929215-78929234 None:intergenic 50.0%
! TGTCTCAGGGACACCGATTA+AGG - chr4.1:78929705-78929724 None:intergenic 50.0%
!! TGTCAGGCACTGAACCTGTT+GGG + chr4.1:78929935-78929954 MS.gene73257:intron 50.0%
!! TTGTCAGGCACTGAACCTGT+TGG + chr4.1:78929934-78929953 MS.gene73257:intron 50.0%
TCAAGGCGGCTAGTGGTGTT+CGG - chr4.1:78927967-78927986 None:intergenic 55.0%
TTCTCTCTCAAGGCGGCTAG+TGG - chr4.1:78927974-78927993 None:intergenic 55.0%
CAAGGCGGCTAGTGGTGTTC+GGG - chr4.1:78927966-78927985 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 78927704 78930042 78927704 ID=MS.gene73257
chr4.1 mRNA 78927704 78930042 78927704 ID=MS.gene73257.t1;Parent=MS.gene73257
chr4.1 exon 78927704 78927907 78927704 ID=MS.gene73257.t1.exon1;Parent=MS.gene73257.t1
chr4.1 CDS 78927704 78927907 78927704 ID=cds.MS.gene73257.t1;Parent=MS.gene73257.t1
chr4.1 exon 78928858 78929589 78928858 ID=MS.gene73257.t1.exon2;Parent=MS.gene73257.t1
chr4.1 CDS 78928858 78929589 78928858 ID=cds.MS.gene73257.t1;Parent=MS.gene73257.t1
chr4.1 exon 78929941 78930042 78929941 ID=MS.gene73257.t1.exon3;Parent=MS.gene73257.t1
chr4.1 CDS 78929941 78930042 78929941 ID=cds.MS.gene73257.t1;Parent=MS.gene73257.t1
Gene Sequence

>MS.gene73257

ATGAAGTTGCATGGAGATTTTGGTGAAGAGAGTTATAATTTTGATGATGGTGTTTTTGTTAAGAAAGGAAGTTTGTATTTGATTTTTGATGATTTGAAAGTGTTGCAAAACTCTCCTGGTAATTCAGTTCAGCAACTCATTCAACTTGGATACAAAAACTTTCACAAGTTGACACAAGTGTCCTTGAATGTTGGATTGAAAGAGGTAATATCACTTCTATAAATTTTTAATATATTTTTAGGAAAATGTTTGGTGAACACCCGAACACCACTAGCCGCCTTGAGAGAGAAAAAAATAGAGAGAGTGATGTGATTAAAAGATAGAGAGAGCGAGAATGTTTGGCTTATATATAGTTTTGGTCTTTCTATTTTGTTACATTTATGATTGTGATCTTTATTTTTAAATTCATGAATTTGACCAGTTAATTTACAAAATATCAGATGTTTTCTCCACCTATGTATTTAGGGTCAATTTTTCATGGTATTTATATGGTAATTAAATTGGTAACATGAATATGAGTTATAAAATAAGAATACAATGTTTTACATCAATTTTTTTTTCTTTATATAGATTAATGCATATTTTTATGAAATGAAAGTTTTTTTTAAAAAAAAGTCCGTGTAGAAATATCACGTCATCATTAATTAAGTTGGCACGTAAACATCCCACTTGTCACATCATCAAAATTCTAATTTAAATGTGAGGAAATCATAGTCATTTTAAAAACTGATTTTAAAAATCGTGGATTTGGAAAAAGCAGATGGACCAATGTCACGAATTAGACAAAATAGAGAGGGATGGAGTTCAGTCCCATAAGTGTAGCTCAATTGATAGTTACCAAGGATTTTACGTGTAGAAGTTGGAGTTCGAACCCCGAATTCTCCACTTTTCTACATATAATGTGTGTGAATTTAGTCATTAGACTACTTGACCAACCAAGAGAGAGACTAAAACTGCATTTAAACAAAAAATAATAATTTAGACATGGATTTCTGATTAATGTTTGTAAGACGAGCTTTATAATATATATACTTCCTTAAGTTCTTTAATTAATTTTTAAACATGTGTATGGGAGATATGTGAAACAAGTGTTAGAATGTAATATAAAAATTTCTTAATTTAACAATATGTTTGTGCCTATTTTAACATTCTAGATTTTGGACTTGCTAAAGCAATCATTAATCTCCAAGTCTCCTCTCACTGATATATTTTTGGCCAATGAAGAATTTAAAATGATGTCAACATTCTCGCCAAGACATGGACCAAAATTTAAAAAATGGACTAATATAAAACTTAAGGTAATGGTGAGAAAATCAAAGAAGAAGATACTATATGCTGAAGCTGAGGGAGAATTTGTTGATTTTCTATTAAGTTTCCTCACAACACCAATTGGATCTATTCTAGGACAATTGAATGACAATTTTTCTCTAGGATGTGTGGAAAATTTGCACAAAAGTGTGAAAGAATTCAATCCTTCATGGTTCATAAGGCCTTTAGGTAACCCCTTACCAAATCCAAAGGTGGCTCCTCAATTTGGTTGCATGAAGCAACCACTAATAAATCTTTGTGAAGAAAAAGCTCCTACTTATTGGTATGGTAATGGTGTGATTAAAAATCATATTTGTTATTCCAATGTGAATGGAGTGATATCAAAGCAAAAAAGTCTTGTAAAAAATCCAGAAGCAATGAAACTTTTTGATCCAAGATCACCTTATGGCACAATTGAATCTGCTTTGGGATTTGTGAAGAGGCCATCACTATTTATTGTGTGGGATGATTTGCATGTGACACCATTCGCAAATACTTCAAGCATATCATTTTTGCACAAGATGAATGTACCATTGGATGATTTGGAGGAGCATATAGTGAGCATTGGAGAAACAGAGGTAATTAACAATCATTATTAATTAATTAATTTATCTTGTCCTTGCAAATCTTGATACTTGGATATTTAATGGTTATCTTGTTTTTAACTCAAAACATGCATTTTTTTTTCTTCCTTAATCGGTGTCCCTGAGACACTAGTTAGCTTAACCCAAAAAAAATTAACAAGAAAATAGATATTAAGGTAGACATGTATAAAATAGTTAAAAGTTTATTATATATATATATATATATATATATATATGGTTTACTCATAATAATCATTGATATATTATAAAACTAGGTGGAATTTTCTTTACAAATCCTTTATAACTAGAAGCAAGTGATGATTTTAATTATCTCTACATTGTCAGGCACTGAACCTGTTGGGAGCTTCTTTGACTTCTTCCAAAGAGGCTTTGACAGACGGTTTGTTCTACCTCCTTAAGAATCCAAAGGAAGAAGTTACAGCTTGA
Protein sequence

>MS.gene73257.t1

MKLHGDFGEESYNFDDGVFVKKGSLYLIFDDLKVLQNSPGNSVQQLIQLGYKNFHKLTQVSLNVGLKEILDLLKQSLISKSPLTDIFLANEEFKMMSTFSPRHGPKFKKWTNIKLKVMVRKSKKKILYAEAEGEFVDFLLSFLTTPIGSILGQLNDNFSLGCVENLHKSVKEFNPSWFIRPLGNPLPNPKVAPQFGCMKQPLINLCEEKAPTYWYGNGVIKNHICYSNVNGVISKQKSLVKNPEAMKLFDPRSPYGTIESALGFVKRPSLFIVWDDLHVTPFANTSSISFLHKMNVPLDDLEEHIVSIGETEALNLLGASLTSSKEALTDGLFYLLKNPKEEVTA