AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73817

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73817.t1 MTR_4g079770 95.652 345 14 1 1 344 1 345 0.0 689
MS.gene73817.t1 MTR_4g079470 95.362 345 15 1 1 344 1 345 0.0 658
MS.gene73817.t1 MTR_5g022560 90.145 345 32 2 1 344 1 344 0.0 647
MS.gene73817.t1 MTR_4g107930 89.565 345 35 1 1 344 1 345 0.0 647
MS.gene73817.t1 MTR_4g079800 91.014 345 30 1 1 344 1 345 0.0 630
MS.gene73817.t1 MTR_4g047610 89.565 345 33 3 1 344 1 343 0.0 623
MS.gene73817.t1 MTR_2g075830 77.101 345 74 4 1 344 1 341 0.0 530
MS.gene73817.t1 MTR_4g080700 65.528 322 102 8 26 344 25 340 4.22e-150 426
MS.gene73817.t1 MTR_4g080730 65.217 322 103 7 26 344 25 340 2.43e-149 424
MS.gene73817.t1 MTR_4g080360 53.824 340 151 5 8 344 6 342 1.91e-130 376
MS.gene73817.t1 MTR_4g104370 57.101 338 135 8 10 342 10 342 4.34e-129 374
MS.gene73817.t1 MTR_2g083930 51.862 349 154 5 1 342 1 342 1.76e-126 366
MS.gene73817.t1 MTR_3g075390 54.006 337 146 6 11 342 11 343 4.77e-125 363
MS.gene73817.t1 MTR_4g079440 55.484 310 131 4 34 342 40 343 4.43e-122 355
MS.gene73817.t1 MTR_3g116080 51.744 344 152 7 7 343 11 347 4.48e-120 350
MS.gene73817.t1 MTR_7g078600 57.419 310 120 7 35 342 37 336 2.72e-118 345
MS.gene73817.t1 MTR_1g107550 54.603 315 135 5 33 342 43 354 1.15e-117 348
MS.gene73817.t1 MTR_2g090890 49.854 343 159 5 7 343 1 336 2.20e-117 343
MS.gene73817.t1 MTR_7g079230 51.895 343 155 7 1 342 1 334 1.08e-116 341
MS.gene73817.t1 MTR_4g094918 48.387 341 165 7 10 343 5 341 1.02e-113 334
MS.gene73817.t1 MTR_1g018840 47.397 365 159 8 4 342 3 360 2.79e-111 332
MS.gene73817.t1 MTR_1g018840 47.397 365 159 8 4 342 3 360 3.38e-111 332
MS.gene73817.t1 MTR_3g102220 51.376 327 140 8 28 342 41 360 2.87e-110 330
MS.gene73817.t1 MTR_3g112430 50.161 311 141 5 41 343 37 341 4.38e-102 308
MS.gene73817.t1 MTR_2g015850 54.483 290 124 6 56 342 78 362 1.38e-98 296
MS.gene73817.t1 MTR_2g016180 43.804 347 171 10 12 343 18 355 1.37e-88 275
MS.gene73817.t1 MTR_2g016270 44.315 343 160 11 12 343 18 340 8.71e-87 270
MS.gene73817.t1 MTR_2g067900 44.688 320 154 12 39 343 15 326 5.61e-78 242
MS.gene73817.t1 MTR_2g067560 44.860 321 145 13 39 338 15 324 3.03e-73 243
MS.gene73817.t1 MTR_4g125300 41.234 308 163 10 42 343 53 348 6.98e-70 222
MS.gene73817.t1 MTR_2g064475 38.441 372 163 17 17 343 1 351 1.07e-69 221
MS.gene73817.t1 MTR_2g015920 39.308 318 179 10 34 343 47 358 3.34e-68 218
MS.gene73817.t1 MTR_4g065077 38.006 321 181 9 33 343 44 356 1.69e-66 214
MS.gene73817.t1 MTR_2g066980 34.930 355 169 12 11 343 4 318 6.16e-66 211
MS.gene73817.t1 MTR_7g078630 44.755 286 117 5 29 303 695 950 1.04e-65 223
MS.gene73817.t1 MTR_4g071060 39.172 314 165 10 38 335 45 348 1.26e-64 209
MS.gene73817.t1 MTR_1g023210 37.758 339 169 12 22 335 31 352 7.49e-63 204
MS.gene73817.t1 MTR_8g086470 37.304 319 170 9 33 343 60 356 3.28e-62 202
MS.gene73817.t1 MTR_2g015930 39.252 321 168 12 38 343 50 358 8.29e-62 201
MS.gene73817.t1 MTR_1g023810 45.022 231 118 6 114 341 37 261 1.05e-61 198
MS.gene73817.t1 MTR_1g094135 36.202 337 177 12 24 335 35 358 3.75e-57 190
MS.gene73817.t1 MTR_4g072790 34.007 297 159 8 42 335 50 312 4.58e-44 154
MS.gene73817.t1 MTR_2g015840 49.367 158 75 4 7 161 1 156 1.18e-42 149
MS.gene73817.t1 MTR_2g015840 55.357 112 49 1 232 343 161 271 5.25e-36 132
MS.gene73817.t1 MTR_7g078580 64.286 98 34 1 245 342 4 100 1.46e-41 141
MS.gene73817.t1 MTR_4g072340 33.887 301 158 10 42 335 50 316 3.48e-41 147
MS.gene73817.t1 MTR_4g073660 33.555 301 159 10 42 335 49 315 3.70e-41 147
MS.gene73817.t1 MTR_7g079130 70.000 90 27 0 253 342 7 96 9.52e-41 138
MS.gene73817.t1 MTR_1g025450 38.503 187 90 7 114 296 43 208 1.40e-32 127
MS.gene73817.t1 MTR_7g078310 45.000 140 67 3 83 219 115 247 2.47e-27 108
MS.gene73817.t1 MTR_2g016380 47.107 121 57 4 227 343 18 135 5.72e-27 107
MS.gene73817.t1 MTR_7g079260 61.111 72 12 1 269 340 7 62 9.10e-23 91.7
MS.gene73817.t1 MTR_7g111060 28.889 225 132 7 132 335 113 330 1.43e-22 97.4
MS.gene73817.t1 MTR_7g111050 29.787 235 135 8 132 343 110 337 5.85e-22 95.5
MS.gene73817.t1 MTR_3g029560 27.888 251 146 9 99 343 16 237 5.10e-21 90.9
MS.gene73817.t1 MTR_8g087510 41.985 131 69 5 167 295 23 148 7.93e-21 88.6
MS.gene73817.t1 MTR_2g047960 48.235 85 43 1 135 219 7 90 7.46e-18 78.2
MS.gene73817.t1 MTR_3g082370 26.087 207 133 5 131 322 5 206 2.66e-17 80.5
MS.gene73817.t1 MTR_7g111050 27.692 195 117 6 132 305 105 296 1.21e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73817.t1 AT5G45890 55.072 345 145 5 7 343 3 345 2.41e-134 386
MS.gene73817.t1 AT5G50260 54.783 345 131 7 15 342 5 341 1.21e-129 375
MS.gene73817.t1 AT3G48340 54.070 344 139 7 13 342 4 342 4.61e-123 358
MS.gene73817.t1 AT2G34080 50.292 342 158 6 11 344 8 345 3.19e-120 350
MS.gene73817.t1 AT4G35350 53.651 315 134 6 34 343 45 352 3.12e-117 343
MS.gene73817.t1 AT3G49340 50.437 343 150 7 17 344 4 341 7.46e-116 339
MS.gene73817.t1 AT3G19390 49.854 343 161 6 9 343 6 345 7.77e-116 343
MS.gene73817.t1 AT3G48350 51.009 347 152 8 11 343 1 343 2.06e-115 339
MS.gene73817.t1 AT5G43060 53.374 326 137 7 25 342 34 352 4.79e-115 342
MS.gene73817.t1 AT2G27420 48.286 350 161 7 10 344 4 348 8.96e-114 334
MS.gene73817.t1 AT1G06260 51.884 345 146 7 8 342 7 341 9.10e-113 331
MS.gene73817.t1 AT1G20850 51.757 313 144 5 34 343 45 353 1.94e-110 326
MS.gene73817.t1 AT4G36880 50.156 321 147 6 33 342 42 360 4.82e-110 326
MS.gene73817.t1 AT1G29090 45.892 353 175 7 4 344 7 355 9.19e-110 324
MS.gene73817.t1 AT3G19400 50.489 307 147 5 40 343 44 348 1.33e-109 324
MS.gene73817.t1 AT1G47128 51.456 309 137 7 40 342 50 351 3.54e-109 327
MS.gene73817.t1 AT1G29080 45.272 349 165 8 13 344 7 346 2.91e-107 317
MS.gene73817.t1 AT1G09850 50.000 310 151 4 34 342 26 332 8.13e-103 309
MS.gene73817.t1 AT4G23520 48.220 309 145 8 41 342 48 348 3.73e-99 297
MS.gene73817.t1 AT4G11310 47.910 311 144 7 41 342 50 351 1.35e-97 293
MS.gene73817.t1 AT4G11320 46.349 315 143 7 41 342 57 358 2.83e-96 290
MS.gene73817.t1 AT4G11320 46.349 315 143 7 41 342 57 358 2.83e-96 290
MS.gene73817.t1 AT1G29110 41.026 351 169 9 9 343 5 333 4.87e-87 265
MS.gene73817.t1 AT3G43960 43.202 331 180 7 17 342 18 345 3.24e-84 259
MS.gene73817.t1 AT4G35350 51.004 249 110 6 34 277 45 286 8.75e-83 253
MS.gene73817.t1 AT3G19400 48.548 241 120 4 40 277 44 283 7.57e-76 235
MS.gene73817.t1 AT3G48350 46.975 281 131 8 11 277 1 277 1.87e-75 234
MS.gene73817.t1 AT5G60360 43.322 307 158 9 42 343 61 356 2.13e-75 236
MS.gene73817.t1 AT5G60360 42.997 307 158 10 42 343 61 355 9.57e-73 229
MS.gene73817.t1 AT3G45310 41.883 308 163 9 42 344 61 357 4.13e-72 228
MS.gene73817.t1 AT5G60360 42.424 297 159 8 42 333 61 350 4.41e-71 225
MS.gene73817.t1 AT3G45310 41.558 308 163 10 42 344 61 356 1.45e-69 221
MS.gene73817.t1 AT2G21430 38.158 304 164 8 47 335 55 349 1.13e-62 204
MS.gene73817.t1 AT4G39090 39.286 308 155 11 47 335 58 352 6.26e-62 202
MS.gene73817.t1 AT3G54940 38.924 316 155 10 43 335 54 354 1.63e-60 198
MS.gene73817.t1 AT4G16190 38.585 311 159 11 45 335 60 358 3.59e-59 195
MS.gene73817.t1 AT3G54940 39.301 229 121 5 43 259 54 276 4.08e-45 155
MS.gene73817.t1 AT1G02305 29.778 225 130 7 132 335 116 333 6.40e-24 101
MS.gene73817.t1 AT4G01610 27.063 303 178 12 66 339 46 334 3.95e-21 93.2
MS.gene73817.t1 AT1G02300 29.384 211 123 6 145 335 146 350 6.05e-20 90.1
MS.gene73817.t1 AT4G01610 26.733 303 179 12 66 339 46 334 7.17e-20 89.7
MS.gene73817.t1 AT2G22160 39.130 92 53 2 55 145 9 98 4.02e-14 68.2

Find 95 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATGATTTAATTGATTAT+TGG 0.217020 4.4:+31425664 None:intergenic
TACAAAAGTGGTGTCTTTAC+TGG 0.245613 4.4:-31424758 MS.gene73817:CDS
ACTTCCCAGTACTCAACTTA+TGG 0.259340 4.4:+31425086 None:intergenic
GCATCAATAGCTACGGAAAT+TGG 0.285597 4.4:+31424803 None:intergenic
CTGCAGTGCCTGCTCATTGT+TGG 0.329361 4.4:+31424844 None:intergenic
AGTTCCGTCAACACCTTGAT+AGG 0.330791 4.4:+31424928 None:intergenic
CTTGTTATTGCCAGCATTGT+TGG 0.332876 4.4:+31425451 None:intergenic
AATAACAAGTTATACAAATT+AGG 0.348584 4.4:-31425437 MS.gene73817:CDS
GAATTCATGGGGAACTGATT+GGG 0.351922 4.4:-31424651 MS.gene73817:CDS
AAGAAAGGGAGAACCGTCTT+AGG 0.352861 4.4:-31425508 MS.gene73817:CDS
TCACTATGGCAAAGTCTATA+AGG 0.352872 4.4:-31425537 MS.gene73817:CDS
ATCCATGCAGTTACCATTAC+CGG 0.356686 4.4:-31424884 MS.gene73817:CDS
AAATTCATCATTCAAAATCA+TGG 0.364764 4.4:-31424974 MS.gene73817:CDS
ATTGGTTGGTTAAGAATTCA+TGG 0.370703 4.4:-31424664 MS.gene73817:CDS
ATTAAATCATAGCATTTCAT+TGG 0.375929 4.4:-31425654 MS.gene73817:CDS
AGGTTGTGAGGGTGGCCTTA+TGG 0.393598 4.4:-31425008 MS.gene73817:CDS
CATTGACCTTGGTTCTTCAC+TGG 0.396716 4.4:+31425254 None:intergenic
AGAATTCATGGGGAACTGAT+TGG 0.403396 4.4:-31424652 MS.gene73817:CDS
GGAATCCATAAGTTGAGTAC+TGG 0.422547 4.4:-31425091 MS.gene73817:CDS
AGTAGTTGTCTTTGTGATTG+AGG 0.437424 4.4:+31425340 None:intergenic
AGTGCCTGCTCATTGTTGGC+AGG 0.443934 4.4:+31424848 None:intergenic
TTTCTCCAATCCACTGCTGA+TGG 0.445961 4.4:+31425293 None:intergenic
ACTCAACTTATGGATTCCTT+CGG 0.446108 4.4:+31425096 None:intergenic
TCATAGCATTTCATTGGCAT+TGG 0.455936 4.4:-31425648 MS.gene73817:CDS
GGTTACGGTGTCGGTAATGA+TGG 0.459653 4.4:-31424695 MS.gene73817:CDS
ATTCTTAACCAACCAATACT+TGG 0.462766 4.4:+31424670 None:intergenic
GACTTTCAGTTCTACAAAAG+TGG 0.468517 4.4:-31424770 MS.gene73817:CDS
GGAACTGATTGGGGTGAAGA+AGG 0.474210 4.4:-31424641 MS.gene73817:CDS
AGCATCTAGAAACAAATTCA+AGG 0.484430 4.4:-31425375 MS.gene73817:CDS
AGATGCTATGAACTCCTCAT+TGG 0.488280 4.4:+31425391 None:intergenic
TTGCTCGTGTCTCTCGTACA+TGG 0.488893 4.4:+31425568 None:intergenic
TCTATAAGGATTCTCAAGAA+AGG 0.490614 4.4:-31425523 MS.gene73817:CDS
AAGTTTGACACTTAAGCAGT+AGG 0.491983 4.4:+31424548 None:intergenic
TACCCCTATCAAGGTGTTGA+CGG 0.498791 4.4:-31424932 MS.gene73817:CDS
GGGGTGAAGAAGGATACATT+AGG 0.500551 4.4:-31424631 MS.gene73817:CDS
GACATCTTCATACCCGGTAA+TGG 0.502057 4.4:+31424871 None:intergenic
AGGATACATTAGGATGCAAA+GGG 0.504604 4.4:-31424621 MS.gene73817:CDS
CTATAAGGATTCTCAAGAAA+GGG 0.505602 4.4:-31425522 MS.gene73817:CDS
ATTTGAAAGCATCATCCATA+AGG 0.506586 4.4:+31424993 None:intergenic
GCAGTGGATTGGAGAAAGAA+AGG 0.507676 4.4:-31425287 MS.gene73817:CDS
GATGCTGCTGAAGGACTTTG+TGG 0.515605 4.4:-31424593 MS.gene73817:CDS
TCTCGTACATGGAGTCATCT+TGG 0.518580 4.4:+31425579 None:intergenic
TAAGTTGAGTACTGGGAAGT+TGG 0.520530 4.4:-31425083 MS.gene73817:CDS
GTGTTCCATCAGCAGTGGAT+TGG 0.522985 4.4:-31425298 MS.gene73817:CDS
GAGCAATGGATGAGTCACTA+TGG 0.524213 4.4:-31425551 MS.gene73817:CDS
TTGGTTGGTTAAGAATTCAT+GGG 0.526242 4.4:-31424663 MS.gene73817:CDS
GACACTTACCACATTGACCT+TGG 0.526963 4.4:+31425243 None:intergenic
AGTTTGACACTTAAGCAGTA+GGG 0.532124 4.4:+31424549 None:intergenic
ATTGAAGCTTCCAACAATGC+TGG 0.532605 4.4:-31425461 MS.gene73817:CDS
CTTGTTGATTGTGACACAAA+AGG 0.533495 4.4:-31425040 MS.gene73817:CDS
AAGGATACATTAGGATGCAA+AGG 0.533659 4.4:-31424622 MS.gene73817:CDS
TCTGCTGTTGCGGCAACCGA+AGG 0.538022 4.4:-31425112 MS.gene73817:CDS
GTTCCGTCAACACCTTGATA+GGG 0.541629 4.4:+31424929 None:intergenic
TAATTGATTATTGGCAGCCA+TGG 0.542700 4.4:+31425673 None:intergenic
GAATCCATAAGTTGAGTACT+GGG 0.546014 4.4:-31425090 MS.gene73817:CDS
GTGCCTGCTCATTGTTGGCA+GGG 0.546537 4.4:+31424849 None:intergenic
TCCGTAGCTATTGATGCTAG+TGG 0.550787 4.4:-31424797 MS.gene73817:CDS
GGTGTCACTGCTGTAGGTTA+CGG 0.557743 4.4:-31424710 MS.gene73817:CDS
GAAGCTCAATACCCCTATCA+AGG 0.559311 4.4:-31424941 MS.gene73817:CDS
TGTCCCTGCCAACAATGAGC+AGG 0.564412 4.4:-31424852 MS.gene73817:CDS
CCGTAGCTATTGATGCTAGT+GGG 0.567886 4.4:-31424796 MS.gene73817:CDS
AGGGGAGTCGATGCTGCTGA+AGG 0.575211 4.4:-31424602 MS.gene73817:CDS
GTAATGATGGGACCAAGTAT+TGG 0.576103 4.4:-31424682 MS.gene73817:CDS
TGATGGGACCAAGTATTGGT+TGG 0.576390 4.4:-31424678 MS.gene73817:CDS
AATTCATGGGGAACTGATTG+GGG 0.577168 4.4:-31424650 MS.gene73817:CDS
GTTACGGTGTCGGTAATGAT+GGG 0.577892 4.4:-31424694 MS.gene73817:CDS
TTTCAGTGAATATCCTAAGA+CGG 0.585706 4.4:+31425495 None:intergenic
AGGACTTTGTGGCATTGCAA+TGG 0.587668 4.4:-31424582 MS.gene73817:CDS
GGTGTAGACCAAGGTTGTGA+GGG 0.591635 4.4:-31425019 MS.gene73817:CDS
GTAGACCAAGGTTGTGAGGG+TGG 0.591695 4.4:-31425016 MS.gene73817:CDS
TAAGGCCACCCTCACAACCT+TGG 0.592405 4.4:+31425011 None:intergenic
AGGTGTAGACCAAGGTTGTG+AGG 0.594253 4.4:-31425020 MS.gene73817:CDS
CCCACTAGCATCAATAGCTA+CGG 0.596676 4.4:+31424796 None:intergenic
GGTAATGGTAACTGCATGGA+TGG 0.597553 4.4:+31424886 None:intergenic
ACCCGGTAATGGTAACTGCA+TGG 0.598149 4.4:+31424882 None:intergenic
GCATCTAGAAACAAATTCAA+GGG 0.601852 4.4:-31425374 MS.gene73817:CDS
TGCAAGTGTTCCATCAGCAG+TGG 0.603452 4.4:-31425303 MS.gene73817:CDS
TTCCGTCAACACCTTGATAG+GGG 0.605371 4.4:+31424930 None:intergenic
GGCAGGGACATCTTCATACC+CGG 0.606676 4.4:+31424865 None:intergenic
TGTACGAGAGACACGAGCAA+TGG 0.611237 4.4:-31425565 MS.gene73817:CDS
TGGTTCATGTGGAACTGAGT+TGG 0.612813 4.4:-31424738 MS.gene73817:CDS
GACCATGGTGTCACTGCTGT+AGG 0.612821 4.4:-31424716 MS.gene73817:CDS
TCCATGCAGTTACCATTACC+GGG 0.614442 4.4:-31424883 MS.gene73817:CDS
ACTGCTGTAGGTTACGGTGT+CGG 0.617674 4.4:-31424704 MS.gene73817:CDS
TGGTTGGTTAAGAATTCATG+GGG 0.622660 4.4:-31424662 MS.gene73817:CDS
GGTGTCTTTACTGGTTCATG+TGG 0.637602 4.4:-31424749 MS.gene73817:CDS
CTATGAACTCCTCATTGGTG+AGG 0.645140 4.4:+31425396 None:intergenic
AACCTACAGCAGTGACACCA+TGG 0.663029 4.4:+31424714 None:intergenic
ATTTGCAGACCTCACCAATG+AGG 0.664553 4.4:-31425405 MS.gene73817:CDS
GTGAAGAACCAAGGTCAATG+TGG 0.671761 4.4:-31425251 MS.gene73817:intron
GCAGGCACTGCAGAAAGCTG+TGG 0.675386 4.4:-31424834 MS.gene73817:CDS
TGTGGAACTGAGTTGGACCA+TGG 0.679648 4.4:-31424731 MS.gene73817:CDS
GACACAAAAGGTGTAGACCA+AGG 0.707755 4.4:-31425028 MS.gene73817:CDS
GTAACACCAGTGAAGAACCA+AGG 0.718576 4.4:-31425260 MS.gene73817:CDS
GGATACATTAGGATGCAAAG+GGG 0.764149 4.4:-31424620 MS.gene73817:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACAAGTTATACAAATT+AGG - chr4.4:31424793-31424812 MS.gene73817:CDS 15.0%
!!! AATTTTAATTTACCTTTTGT+AGG - chr4.4:31425079-31425098 MS.gene73817:CDS 15.0%
!! AAATTCATCATTCAAAATCA+TGG - chr4.4:31425256-31425275 MS.gene73817:CDS 20.0%
!! ATTAAATCATAGCATTTCAT+TGG - chr4.4:31424576-31424595 MS.gene73817:CDS 20.0%
!! GCTATGATTTAATTGATTAT+TGG + chr4.4:31424569-31424588 None:intergenic 20.0%
AGCATCTAGAAACAAATTCA+AGG - chr4.4:31424855-31424874 MS.gene73817:CDS 30.0%
ATTCTTAACCAACCAATACT+TGG + chr4.4:31425563-31425582 None:intergenic 30.0%
CTATAAGGATTCTCAAGAAA+GGG - chr4.4:31424708-31424727 MS.gene73817:CDS 30.0%
GCATCTAGAAACAAATTCAA+GGG - chr4.4:31424856-31424875 MS.gene73817:CDS 30.0%
TCTATAAGGATTCTCAAGAA+AGG - chr4.4:31424707-31424726 MS.gene73817:CDS 30.0%
TGGTAAGTGTCTATCTAATA+TGG - chr4.4:31424999-31425018 MS.gene73817:CDS 30.0%
TTTCAGTGAATATCCTAAGA+CGG + chr4.4:31424738-31424757 None:intergenic 30.0%
! ATTGGTTGGTTAAGAATTCA+TGG - chr4.4:31425566-31425585 MS.gene73817:CDS 30.0%
! TTGGTTGGTTAAGAATTCAT+GGG - chr4.4:31425567-31425586 MS.gene73817:CDS 30.0%
!! ATTTGAAAGCATCATCCATA+AGG + chr4.4:31425240-31425259 None:intergenic 30.0%
AAGGATACATTAGGATGCAA+AGG - chr4.4:31425608-31425627 MS.gene73817:CDS 35.0%
ACTCAACTTATGGATTCCTT+CGG + chr4.4:31425137-31425156 None:intergenic 35.0%
AGGATACATTAGGATGCAAA+GGG - chr4.4:31425609-31425628 MS.gene73817:CDS 35.0%
AGTAGTTGTCTTTGTGATTG+AGG + chr4.4:31424893-31424912 None:intergenic 35.0%
GACTTTCAGTTCTACAAAAG+TGG - chr4.4:31425460-31425479 MS.gene73817:CDS 35.0%
TACAAAAGTGGTGTCTTTAC+TGG - chr4.4:31425472-31425491 MS.gene73817:CDS 35.0%
TCACTATGGCAAAGTCTATA+AGG - chr4.4:31424693-31424712 MS.gene73817:CDS 35.0%
TCATAGCATTTCATTGGCAT+TGG - chr4.4:31424582-31424601 MS.gene73817:CDS 35.0%
! CTTGTTGATTGTGACACAAA+AGG - chr4.4:31425190-31425209 MS.gene73817:intron 35.0%
! GAATCCATAAGTTGAGTACT+GGG - chr4.4:31425140-31425159 MS.gene73817:intron 35.0%
! TGGTTGGTTAAGAATTCATG+GGG - chr4.4:31425568-31425587 MS.gene73817:CDS 35.0%
!!! ATTGGCATTGGTTTTATGCT+TGG - chr4.4:31424594-31424613 MS.gene73817:CDS 35.0%
!!! TTGGCATTGGTTTTATGCTT+GGG - chr4.4:31424595-31424614 MS.gene73817:CDS 35.0%
AATTCATGGGGAACTGATTG+GGG - chr4.4:31425580-31425599 MS.gene73817:CDS 40.0%
ACTTCCCAGTACTCAACTTA+TGG + chr4.4:31425147-31425166 None:intergenic 40.0%
AGAATTCATGGGGAACTGAT+TGG - chr4.4:31425578-31425597 MS.gene73817:CDS 40.0%
AGATGCTATGAACTCCTCAT+TGG + chr4.4:31424842-31424861 None:intergenic 40.0%
ATCCATGCAGTTACCATTAC+CGG - chr4.4:31425346-31425365 MS.gene73817:CDS 40.0%
ATTGAAGCTTCCAACAATGC+TGG - chr4.4:31424769-31424788 MS.gene73817:CDS 40.0%
GAATTCATGGGGAACTGATT+GGG - chr4.4:31425579-31425598 MS.gene73817:CDS 40.0%
GCATCAATAGCTACGGAAAT+TGG + chr4.4:31425430-31425449 None:intergenic 40.0%
GGATACATTAGGATGCAAAG+GGG - chr4.4:31425610-31425629 MS.gene73817:CDS 40.0%
! ACCTTTTGTAGGATGTTGCT+GGG - chr4.4:31425090-31425109 MS.gene73817:CDS 40.0%
! GGAATCCATAAGTTGAGTAC+TGG - chr4.4:31425139-31425158 MS.gene73817:intron 40.0%
! GTAATGATGGGACCAAGTAT+TGG - chr4.4:31425548-31425567 MS.gene73817:CDS 40.0%
! TACCTTTTGTAGGATGTTGC+TGG - chr4.4:31425089-31425108 MS.gene73817:CDS 40.0%
!! CTTGTTATTGCCAGCATTGT+TGG + chr4.4:31424782-31424801 None:intergenic 40.0%
!! GGTTTTATGCTTGGGATTGT+TGG - chr4.4:31424603-31424622 MS.gene73817:CDS 40.0%
!! TAAGTTGAGTACTGGGAAGT+TGG - chr4.4:31425147-31425166 MS.gene73817:intron 40.0%
AAGAAAGGGAGAACCGTCTT+AGG - chr4.4:31424722-31424741 MS.gene73817:CDS 45.0%
AGGACTTTGTGGCATTGCAA+TGG - chr4.4:31425648-31425667 MS.gene73817:CDS 45.0%
AGTTCCGTCAACACCTTGAT+AGG + chr4.4:31425305-31425324 None:intergenic 45.0%
ATTTGCAGACCTCACCAATG+AGG - chr4.4:31424825-31424844 MS.gene73817:CDS 45.0%
CCCACTAGCATCAATAGCTA+CGG + chr4.4:31425437-31425456 None:intergenic 45.0%
CCGTAGCTATTGATGCTAGT+GGG - chr4.4:31425434-31425453 MS.gene73817:CDS 45.0%
CTATGAACTCCTCATTGGTG+AGG + chr4.4:31424837-31424856 None:intergenic 45.0%
GAAGCTCAATACCCCTATCA+AGG - chr4.4:31425289-31425308 MS.gene73817:CDS 45.0%
GACACTTACCACATTGACCT+TGG + chr4.4:31424990-31425009 None:intergenic 45.0%
GACATCTTCATACCCGGTAA+TGG + chr4.4:31425362-31425381 None:intergenic 45.0%
GAGCAATGGATGAGTCACTA+TGG - chr4.4:31424679-31424698 MS.gene73817:CDS 45.0%
GGGGTGAAGAAGGATACATT+AGG - chr4.4:31425599-31425618 MS.gene73817:CDS 45.0%
GTAACACCAGTGAAGAACCA+AGG - chr4.4:31424970-31424989 MS.gene73817:CDS 45.0%
GTGAAGAACCAAGGTCAATG+TGG - chr4.4:31424979-31424998 MS.gene73817:CDS 45.0%
GTTCCGTCAACACCTTGATA+GGG + chr4.4:31425304-31425323 None:intergenic 45.0%
TCCATGCAGTTACCATTACC+GGG - chr4.4:31425347-31425366 MS.gene73817:CDS 45.0%
TCCGTAGCTATTGATGCTAG+TGG - chr4.4:31425433-31425452 MS.gene73817:CDS 45.0%
TCTCGTACATGGAGTCATCT+TGG + chr4.4:31424654-31424673 None:intergenic 45.0%
TGGTTCATGTGGAACTGAGT+TGG - chr4.4:31425492-31425511 MS.gene73817:CDS 45.0%
TTCCGTCAACACCTTGATAG+GGG + chr4.4:31425303-31425322 None:intergenic 45.0%
TTTCTCCAATCCACTGCTGA+TGG + chr4.4:31424940-31424959 None:intergenic 45.0%
! CATTGACCTTGGTTCTTCAC+TGG + chr4.4:31424979-31424998 None:intergenic 45.0%
! GACACAAAAGGTGTAGACCA+AGG - chr4.4:31425202-31425221 MS.gene73817:intron 45.0%
! GGTAATGGTAACTGCATGGA+TGG + chr4.4:31425347-31425366 None:intergenic 45.0%
!! GCAGTGGATTGGAGAAAGAA+AGG - chr4.4:31424943-31424962 MS.gene73817:CDS 45.0%
!! GGTGTCTTTACTGGTTCATG+TGG - chr4.4:31425481-31425500 MS.gene73817:CDS 45.0%
!! GTTACGGTGTCGGTAATGAT+GGG - chr4.4:31425536-31425555 MS.gene73817:CDS 45.0%
!! TACCCCTATCAAGGTGTTGA+CGG - chr4.4:31425298-31425317 MS.gene73817:CDS 45.0%
!! TGATGGGACCAAGTATTGGT+TGG - chr4.4:31425552-31425571 MS.gene73817:CDS 45.0%
AACCTACAGCAGTGACACCA+TGG + chr4.4:31425519-31425538 None:intergenic 50.0%
ACTGCTGTAGGTTACGGTGT+CGG - chr4.4:31425526-31425545 MS.gene73817:CDS 50.0%
AGGTGTAGACCAAGGTTGTG+AGG - chr4.4:31425210-31425229 MS.gene73817:intron 50.0%
GATGCTGCTGAAGGACTTTG+TGG - chr4.4:31425637-31425656 MS.gene73817:CDS 50.0%
GCCCAGCAACATCCTACAAA+AGG + chr4.4:31425094-31425113 None:intergenic 50.0%
GGAACTGATTGGGGTGAAGA+AGG - chr4.4:31425589-31425608 MS.gene73817:CDS 50.0%
GGTGTAGACCAAGGTTGTGA+GGG - chr4.4:31425211-31425230 MS.gene73817:intron 50.0%
GTGTTCCATCAGCAGTGGAT+TGG - chr4.4:31424932-31424951 MS.gene73817:CDS 50.0%
TGCAAGTGTTCCATCAGCAG+TGG - chr4.4:31424927-31424946 MS.gene73817:CDS 50.0%
TGTACGAGAGACACGAGCAA+TGG - chr4.4:31424665-31424684 MS.gene73817:CDS 50.0%
TTGCTCGTGTCTCTCGTACA+TGG + chr4.4:31424665-31424684 None:intergenic 50.0%
! ACCCGGTAATGGTAACTGCA+TGG + chr4.4:31425351-31425370 None:intergenic 50.0%
! CTGGGCATTTTCTGCTGTTG+CGG - chr4.4:31425108-31425127 MS.gene73817:CDS 50.0%
! TGTGGAACTGAGTTGGACCA+TGG - chr4.4:31425499-31425518 MS.gene73817:CDS 50.0%
!! GGTGTCACTGCTGTAGGTTA+CGG - chr4.4:31425520-31425539 MS.gene73817:CDS 50.0%
!! GGTTACGGTGTCGGTAATGA+TGG - chr4.4:31425535-31425554 MS.gene73817:CDS 50.0%
AGTGCCTGCTCATTGTTGGC+AGG + chr4.4:31425385-31425404 None:intergenic 55.0%
CTGCAGTGCCTGCTCATTGT+TGG + chr4.4:31425389-31425408 None:intergenic 55.0%
GGCAGGGACATCTTCATACC+CGG + chr4.4:31425368-31425387 None:intergenic 55.0%
GTAGACCAAGGTTGTGAGGG+TGG - chr4.4:31425214-31425233 MS.gene73817:intron 55.0%
GTGCCTGCTCATTGTTGGCA+GGG + chr4.4:31425384-31425403 None:intergenic 55.0%
TAAGGCCACCCTCACAACCT+TGG + chr4.4:31425222-31425241 None:intergenic 55.0%
TGTCCCTGCCAACAATGAGC+AGG - chr4.4:31425378-31425397 MS.gene73817:CDS 55.0%
! AGGTTGTGAGGGTGGCCTTA+TGG - chr4.4:31425222-31425241 MS.gene73817:intron 55.0%
!! GACCATGGTGTCACTGCTGT+AGG - chr4.4:31425514-31425533 MS.gene73817:CDS 55.0%
AGGGGAGTCGATGCTGCTGA+AGG - chr4.4:31425628-31425647 MS.gene73817:CDS 60.0%
TCTGCTGTTGCGGCAACCGA+AGG - chr4.4:31425118-31425137 MS.gene73817:CDS 60.0%
! GCAGGCACTGCAGAAAGCTG+TGG - chr4.4:31425396-31425415 MS.gene73817:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 31424559 31425693 31424559 ID=MS.gene73817
chr4.4 mRNA 31424559 31425693 31424559 ID=MS.gene73817.t1;Parent=MS.gene73817
chr4.4 exon 31425252 31425693 31425252 ID=MS.gene73817.t1.exon1;Parent=MS.gene73817.t1
chr4.4 CDS 31425252 31425693 31425252 ID=cds.MS.gene73817.t1;Parent=MS.gene73817.t1
chr4.4 exon 31424559 31425151 31424559 ID=MS.gene73817.t1.exon2;Parent=MS.gene73817.t1
chr4.4 CDS 31424559 31425151 31424559 ID=cds.MS.gene73817.t1;Parent=MS.gene73817.t1
Gene Sequence

>MS.gene73817

ATGGCTGCCAATAATCAATTAAATCATAGCATTTCATTGGCATTGGTTTTATGCTTGGGATTGTTGGCTATTCAAGTCACTTCTCGTACTCTCCAAGATGACTCCATGTACGAGAGACACGAGCAATGGATGAGTCACTATGGCAAAGTCTATAAGGATTCTCAAGAAAGGGAGAACCGTCTTAGGATATTCACTGAAAATGTGAATTACATTGAAGCTTCCAACAATGCTGGCAATAACAAGTTATACAAATTAGGCATTAATCAATTTGCAGACCTCACCAATGAGGAGTTCATAGCATCTAGAAACAAATTCAAGGGTCATATGTGCTCCTCAATCACAAAGACAACTACTTTTATGTATGAAAATGCAAGTGTTCCATCAGCAGTGGATTGGAGAAAGAAAGGAGCAGTAACACCAGTGAAGAACCAAGGTCAATGTGGTAAGTGTCTATCTAATATGGAATGCATTCAATTACATATTATGTGATAAAAAAATGAATTGTCATTAAACTCTAGTTAATTTTAATTTACCTTTTGTAGGATGTTGCTGGGCATTTTCTGCTGTTGCGGCAACCGAAGGAATCCATAAGTTGAGTACTGGGAAGTTGGTCTCTTTATCAGAACAAGAACTTGTTGATTGTGACACAAAAGGTGTAGACCAAGGTTGTGAGGGTGGCCTTATGGATGATGCTTTCAAATTCATCATTCAAAATCATGGACTCAACACTGAAGCTCAATACCCCTATCAAGGTGTTGACGGAACTTGCAGTGCAAACAAAGCATCCATCCATGCAGTTACCATTACCGGGTATGAAGATGTCCCTGCCAACAATGAGCAGGCACTGCAGAAAGCTGTGGCAAATCAACCAATTTCCGTAGCTATTGATGCTAGTGGGTCTGACTTTCAGTTCTACAAAAGTGGTGTCTTTACTGGTTCATGTGGAACTGAGTTGGACCATGGTGTCACTGCTGTAGGTTACGGTGTCGGTAATGATGGGACCAAGTATTGGTTGGTTAAGAATTCATGGGGAACTGATTGGGGTGAAGAAGGATACATTAGGATGCAAAGGGGAGTCGATGCTGCTGAAGGACTTTGTGGCATTGCAATGGAAGCATCTTACCCTACTGCTTAA
Protein sequence

>MS.gene73817.t1

MAANNQLNHSISLALVLCLGLLAIQVTSRTLQDDSMYERHEQWMSHYGKVYKDSQERENRLRIFTENVNYIEASNNAGNNKLYKLGINQFADLTNEEFIASRNKFKGHMCSSITKTTTFMYENASVPSAVDWRKKGAVTPVKNQGQCGCCWAFSAVAATEGIHKLSTGKLVSLSEQELVDCDTKGVDQGCEGGLMDDAFKFIIQNHGLNTEAQYPYQGVDGTCSANKASIHAVTITGYEDVPANNEQALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGVGNDGTKYWLVKNSWGTDWGEEGYIRMQRGVDAAEGLCGIAMEASYPTA