AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76035


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76035.t1 MTR_0024s0060 95.652 69 3 0 1 69 58 126 1.34e-44 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76035.t1 AT5G16060 75.362 69 17 0 1 69 1 69 3.44e-34 113

Find 22 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATGGAGCAGGTCGGGCT+AGG 0.306635 6.3:+13973111 MS.gene76035:CDS
GGAGAACACTTTCTGTTGTT+TGG 0.342847 6.3:+13972453 MS.gene76035:CDS
GGATGGAGCAGGTCGGGCTA+GGG 0.356178 6.3:+13973112 MS.gene76035:CDS
GATGGAGCAGGTCGGGCTAG+GGG 0.389420 6.3:+13973113 MS.gene76035:CDS
TGTAGGGGATGGAGCAGGTC+GGG 0.401502 6.3:+13973106 MS.gene76035:CDS
TAAAATTAGCAGGCTGTGTA+GGG 0.412666 6.3:+13973090 MS.gene76035:CDS
ATAAAATTAGCAGGCTGTGT+AGG 0.488137 6.3:+13973089 MS.gene76035:CDS
GTGTAGGGGATGGAGCAGGT+CGG 0.516785 6.3:+13973105 MS.gene76035:CDS
TTTCTGTTGTTTGGCAATGT+CGG 0.528813 6.3:+13972462 MS.gene76035:CDS
GGCTGTGTAGGGGATGGAGC+AGG 0.533138 6.3:+13973101 MS.gene76035:CDS
GAAATATGCTGAGTGTGCTA+TGG 0.542058 6.3:+13972430 MS.gene76035:CDS
AGCTTTACGGAGTAAAATGA+AGG 0.543630 6.3:+13972373 MS.gene76035:intron
AAATATGCTGAGTGTGCTAT+GGG 0.546403 6.3:+13972431 MS.gene76035:CDS
TCCTCAAAGTTTGAAATGGG+TGG 0.549867 6.3:+13971544 None:intergenic
GGTGCGACTATAAAATTAGC+AGG 0.551846 6.3:+13973080 MS.gene76035:CDS
TTAGCAGGCTGTGTAGGGGA+TGG 0.586345 6.3:+13973095 MS.gene76035:CDS
ACGGAGTAAAATGAAGGTGA+AGG 0.589183 6.3:+13972379 MS.gene76035:CDS
GTGAAGAAGAAAGTCGAAGA+AGG 0.598683 6.3:+13971595 MS.gene76035:CDS
GGGCTAGGGGTGTAAAGCAA+AGG 0.610557 6.3:+13973126 MS.gene76035:CDS
TTTGTTAGATATCAACATGG+AGG 0.646725 6.3:+13973059 MS.gene76035:intron
AAAATTAGCAGGCTGTGTAG+GGG 0.679077 6.3:+13973091 MS.gene76035:CDS
AATATGCTGAGTGTGCTATG+GGG 0.810113 6.3:+13972432 MS.gene76035:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATTTTTTTGTTTTATTTT+GGG + chr6.3:13972560-13972579 MS.gene76035:intron 10.0%
!!! TTGATTTTTTTGTTTTATTT+TGG + chr6.3:13972559-13972578 MS.gene76035:intron 10.0%
!! ATATACATTATTCCATAAAT+GGG + chr6.3:13971722-13971741 MS.gene76035:intron 15.0%
!! CATATTATTCAAATGAATAA+GGG - chr6.3:13972084-13972103 None:intergenic 15.0%
!! TATATACATTATTCCATAAA+TGG + chr6.3:13971721-13971740 MS.gene76035:intron 15.0%
!!! ATTTTATAGTTAAATTGATG+TGG + chr6.3:13971765-13971784 MS.gene76035:intron 15.0%
!!! CAATTTTTATGAACAAAAAT+GGG + chr6.3:13971675-13971694 MS.gene76035:intron 15.0%
!!! TCAATTTTTATGAACAAAAA+TGG + chr6.3:13971674-13971693 MS.gene76035:intron 15.0%
!! ACTTTATGATGAAGAAAAAA+TGG - chr6.3:13971653-13971672 None:intergenic 20.0%
!! CTTTATGATGAAGAAAAAAT+GGG - chr6.3:13971652-13971671 None:intergenic 20.0%
!! GCATATTATTCAAATGAATA+AGG - chr6.3:13972085-13972104 None:intergenic 20.0%
!!! AAAAATGGGTTTATTTCAAA+TGG + chr6.3:13971689-13971708 MS.gene76035:intron 20.0%
!!! AAAATGGGTTTATTTCAAAT+GGG + chr6.3:13971690-13971709 MS.gene76035:intron 20.0%
!!! ATCATCATTTTCATTCAATT+GGG + chr6.3:13971794-13971813 MS.gene76035:intron 20.0%
!!! ATGATCAAAACTTTGTTTAA+TGG + chr6.3:13972316-13972335 MS.gene76035:intron 20.0%
!!! ATTTTTGTTAGATATCAACA+TGG + chr6.3:13973056-13973075 MS.gene76035:intron 20.0%
!!! TATCATCATTTTCATTCAAT+TGG + chr6.3:13971793-13971812 MS.gene76035:intron 20.0%
!!! TCTTTCGTTAGTAATTTTAT+GGG - chr6.3:13972702-13972721 None:intergenic 20.0%
!!! TTTTATTTTGGGTTAAGAAT+TGG + chr6.3:13972571-13972590 MS.gene76035:intron 20.0%
! AAAAAGACATTTATCCAAAC+AGG - chr6.3:13971828-13971847 None:intergenic 25.0%
! AAAAGACATTTATCCAAACA+GGG - chr6.3:13971827-13971846 None:intergenic 25.0%
! GTTAGGGTTTAGTATTATTA+TGG - chr6.3:13971623-13971642 None:intergenic 25.0%
! TATAGTTAAATTGATGTGGT+GGG + chr6.3:13971769-13971788 MS.gene76035:intron 25.0%
! TTATACATAAGTGCTTATCA+TGG - chr6.3:13971876-13971895 None:intergenic 25.0%
! TTATAGTTAAATTGATGTGG+TGG + chr6.3:13971768-13971787 MS.gene76035:intron 25.0%
!! ACTTTGTTTAATGGTGATAT+TGG + chr6.3:13972325-13972344 MS.gene76035:intron 25.0%
!! CATAATTTGAAACCCATTTA+TGG - chr6.3:13971737-13971756 None:intergenic 25.0%
!! GTCTTTCGTTAGTAATTTTA+TGG - chr6.3:13972703-13972722 None:intergenic 25.0%
!! TTTTGTAATTTCGATGCATT+CGG + chr6.3:13972737-13972756 MS.gene76035:intron 25.0%
!!! ATGAAGAAAAAATGGGTTTT+GGG - chr6.3:13971645-13971664 None:intergenic 25.0%
!!! TTCATTTTTCGTTGTGTTTT+TGG + chr6.3:13972255-13972274 MS.gene76035:intron 25.0%
AATGGTGATATTGGAATGAT+GGG + chr6.3:13972334-13972353 MS.gene76035:intron 30.0%
AGAGTATACAATTGATGGAA+GGG + chr6.3:13973019-13973038 MS.gene76035:intron 30.0%
ATCTCACACATTTAGGAAAT+TGG + chr6.3:13972775-13972794 MS.gene76035:intron 30.0%
CTCAAATGACTTCAAAATCT+GGG + chr6.3:13972828-13972847 MS.gene76035:intron 30.0%
GATTACATTCAGCTATGTTA+CGG + chr6.3:13972162-13972181 MS.gene76035:intron 30.0%
TAATGGTGATATTGGAATGA+TGG + chr6.3:13972333-13972352 MS.gene76035:intron 30.0%
TAGAGTATACAATTGATGGA+AGG + chr6.3:13973018-13973037 MS.gene76035:intron 30.0%
TCAAATGACTTCAAAATCTG+GGG + chr6.3:13972829-13972848 MS.gene76035:intron 30.0%
TCCATCAATTGTATACTCTA+AGG - chr6.3:13973018-13973037 None:intergenic 30.0%
TCTCACACATTTAGGAAATT+GGG + chr6.3:13972776-13972795 MS.gene76035:intron 30.0%
TTTGTTAGATATCAACATGG+AGG + chr6.3:13973059-13973078 MS.gene76035:intron 30.0%
! TGATCAATTTTGCAGCTTTA+CGG + chr6.3:13972360-13972379 MS.gene76035:intron 30.0%
!! GATGAAGAAAAAATGGGTTT+TGG - chr6.3:13971646-13971665 None:intergenic 30.0%
!! TGAATGTGTCTTTTGTTTGT+TGG + chr6.3:13972637-13972656 MS.gene76035:intron 30.0%
!!! AAAAAATGGGTTTTGGGTTA+GGG - chr6.3:13971639-13971658 None:intergenic 30.0%
!!! AGATGGGTTTTATTCTAATG+AGG + chr6.3:13972284-13972303 MS.gene76035:intron 30.0%
!!! ATTTTGAAGTCATTTGAGGT+GGG - chr6.3:13972826-13972845 None:intergenic 30.0%
!!! CACGAGTTTTTTTAGAAATG+TGG + chr6.3:13972898-13972917 MS.gene76035:intron 30.0%
AAATATGCTGAGTGTGCTAT+GGG + chr6.3:13972431-13972450 MS.gene76035:CDS 35.0%
ACTAACGAAAGACACGTATA+GGG + chr6.3:13972710-13972729 MS.gene76035:intron 35.0%
AGCTTTACGGAGTAAAATGA+AGG + chr6.3:13972373-13972392 MS.gene76035:intron 35.0%
ATAAAATTAGCAGGCTGTGT+AGG + chr6.3:13973089-13973108 MS.gene76035:CDS 35.0%
ATCTGAGCTTATCTACTGTT+TGG - chr6.3:13972044-13972063 None:intergenic 35.0%
CAAATGACTTCAAAATCTGG+GGG + chr6.3:13972830-13972849 MS.gene76035:intron 35.0%
CACTGTAAAATCTGAAGAGA+TGG - chr6.3:13972952-13972971 None:intergenic 35.0%
CCTCAAATGACTTCAAAATC+TGG + chr6.3:13972827-13972846 MS.gene76035:intron 35.0%
CTCACACATTTAGGAAATTG+GGG + chr6.3:13972777-13972796 MS.gene76035:intron 35.0%
GAAATTGGGGTTTAGTCAAA+GGG + chr6.3:13972790-13972809 MS.gene76035:intron 35.0%
GAGTATACAATTGATGGAAG+GGG + chr6.3:13973020-13973039 MS.gene76035:intron 35.0%
GCCTTAGAGTATACAATTGA+TGG + chr6.3:13973014-13973033 MS.gene76035:intron 35.0%
TAAAATTAGCAGGCTGTGTA+GGG + chr6.3:13973090-13973109 MS.gene76035:CDS 35.0%
TAAATGCATCTTCCCTTTGT+CGG + chr6.3:13972605-13972624 MS.gene76035:intron 35.0%
TACTAACGAAAGACACGTAT+AGG + chr6.3:13972709-13972728 MS.gene76035:intron 35.0%
TCAATAACCAAACCGACAAA+GGG - chr6.3:13972620-13972639 None:intergenic 35.0%
TTAAGTTCATGCCTAAAGCT+AGG + chr6.3:13972987-13973006 MS.gene76035:intron 35.0%
TTCAATAACCAAACCGACAA+AGG - chr6.3:13972621-13972640 None:intergenic 35.0%
! TGAAGAGATGGATTTGACTT+GGG - chr6.3:13972940-13972959 None:intergenic 35.0%
! TTTCTGTTGTTTGGCAATGT+CGG + chr6.3:13972462-13972481 MS.gene76035:CDS 35.0%
!! GCTGTTTTCATAAGCTATCT+TGG + chr6.3:13971945-13971964 MS.gene76035:intron 35.0%
!! GTGTCTTTTGTTTGTTGGTT+TGG + chr6.3:13972642-13972661 MS.gene76035:intron 35.0%
!!! CCAGATTTTGAAGTCATTTG+AGG - chr6.3:13972830-13972849 None:intergenic 35.0%
!!! GAAAAAATGGGTTTTGGGTT+AGG - chr6.3:13971640-13971659 None:intergenic 35.0%
!!! GATTTTGAAGTCATTTGAGG+TGG - chr6.3:13972827-13972846 None:intergenic 35.0%
!!! GTGTTTTTGGAAGAAGAGAT+GGG + chr6.3:13972268-13972287 MS.gene76035:intron 35.0%
!!! TGTGTTTTTGGAAGAAGAGA+TGG + chr6.3:13972267-13972286 MS.gene76035:intron 35.0%
!!! TTTTGAAGTCATTTGAGGTG+GGG - chr6.3:13972825-13972844 None:intergenic 35.0%
AAAATTAGCAGGCTGTGTAG+GGG + chr6.3:13973091-13973110 MS.gene76035:CDS 40.0%
AATATGCTGAGTGTGCTATG+GGG + chr6.3:13972432-13972451 MS.gene76035:CDS 40.0%
ACGGAGTAAAATGAAGGTGA+AGG + chr6.3:13972379-13972398 MS.gene76035:CDS 40.0%
ACTAACCGGATACACATTCA+AGG - chr6.3:13972669-13972688 None:intergenic 40.0%
GAAATATGCTGAGTGTGCTA+TGG + chr6.3:13972430-13972449 MS.gene76035:CDS 40.0%
GGAAATTGGGGTTTAGTCAA+AGG + chr6.3:13972789-13972808 MS.gene76035:intron 40.0%
GGAGAACACTTTCTGTTGTT+TGG + chr6.3:13972453-13972472 MS.gene76035:CDS 40.0%
GGTGCGACTATAAAATTAGC+AGG + chr6.3:13973080-13973099 MS.gene76035:CDS 40.0%
GTGAAGAAGAAAGTCGAAGA+AGG + chr6.3:13971595-13971614 MS.gene76035:CDS 40.0%
GTGACACATCTCACACATTT+AGG + chr6.3:13972768-13972787 MS.gene76035:intron 40.0%
TTGGTCCTTGAATGTGTATC+CGG + chr6.3:13972661-13972680 MS.gene76035:intron 40.0%
! ACGCCTTCTATCAAAAGTGT+CGG - chr6.3:13972117-13972136 None:intergenic 40.0%
! ATGTGTATCCGGTTAGTCAA+AGG + chr6.3:13972672-13972691 MS.gene76035:intron 40.0%
! CTCTTCAGATTTTACAGTGC+CGG + chr6.3:13972953-13972972 MS.gene76035:intron 40.0%
! CTGAAGAGATGGATTTGACT+TGG - chr6.3:13972941-13972960 None:intergenic 40.0%
! GTGTTTCCAGAAGTGTACTT+TGG - chr6.3:13972881-13972900 None:intergenic 40.0%
! TGTGTATCCGGTTAGTCAAA+GGG + chr6.3:13972673-13972692 MS.gene76035:intron 40.0%
!! ATTGGGTTTTAGTCCCTGTT+TGG + chr6.3:13971811-13971830 MS.gene76035:intron 40.0%
!! TATCAAAAGTGTCGGTGCTA+AGG - chr6.3:13972109-13972128 None:intergenic 40.0%
AACGAAAGACACGTATAGGG+AGG + chr6.3:13972713-13972732 MS.gene76035:intron 45.0%
GAACTTAACGTGTAGTGTGC+CGG - chr6.3:13972975-13972994 None:intergenic 45.0%
GCAGTTCCAAAGTACACTTC+TGG + chr6.3:13972872-13972891 MS.gene76035:intron 45.0%
TTCATGCCTAAAGCTAGGTG+AGG + chr6.3:13972992-13973011 MS.gene76035:intron 45.0%
!! AGAGATGGATTTGACTTGGG+AGG - chr6.3:13972937-13972956 None:intergenic 45.0%
!! GCACCGACACTTTTGATAGA+AGG + chr6.3:13972111-13972130 MS.gene76035:intron 45.0%
!!! ACTTGGGAGGCTACTTTTCA+CGG - chr6.3:13972924-13972943 None:intergenic 45.0%
TGCTGTGTCAATGTCGTGTC+TGG + chr6.3:13972188-13972207 MS.gene76035:intron 50.0%
TGGGTCTCCCTTTGACTAAC+CGG - chr6.3:13972683-13972702 None:intergenic 50.0%
! CTAAGGCCTCACCTAGCTTT+AGG - chr6.3:13973001-13973020 None:intergenic 50.0%
! GCATCTTCCCTTTGTCGGTT+TGG + chr6.3:13972610-13972629 MS.gene76035:intron 50.0%
GTCGTGTCTGGTGTCTGTGT+CGG + chr6.3:13972200-13972219 MS.gene76035:intron 55.0%
TTAGCAGGCTGTGTAGGGGA+TGG + chr6.3:13973095-13973114 MS.gene76035:CDS 55.0%
! GGGCTAGGGGTGTAAAGCAA+AGG + chr6.3:13973126-13973145 MS.gene76035:CDS 55.0%
GTGTAGGGGATGGAGCAGGT+CGG + chr6.3:13973105-13973124 MS.gene76035:CDS 60.0%
TGTAGGGGATGGAGCAGGTC+GGG + chr6.3:13973106-13973125 MS.gene76035:CDS 60.0%
GGATGGAGCAGGTCGGGCTA+GGG + chr6.3:13973112-13973131 MS.gene76035:CDS 65.0%
GGCTGTGTAGGGGATGGAGC+AGG + chr6.3:13973101-13973120 MS.gene76035:CDS 65.0%
! GATGGAGCAGGTCGGGCTAG+GGG + chr6.3:13973113-13973132 MS.gene76035:CDS 65.0%
GGGATGGAGCAGGTCGGGCT+AGG + chr6.3:13973111-13973130 MS.gene76035:CDS 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 13971559 13973157 13971559 ID=MS.gene76035
chr6.3 mRNA 13971559 13973157 13971559 ID=MS.gene76035.t1;Parent=MS.gene76035
chr6.3 exon 13971559 13971616 13971559 ID=MS.gene76035.t1.exon1;Parent=MS.gene76035.t1
chr6.3 CDS 13971559 13971616 13971559 ID=cds.MS.gene76035.t1;Parent=MS.gene76035.t1
chr6.3 exon 13972375 13972522 13972375 ID=MS.gene76035.t1.exon2;Parent=MS.gene76035.t1
chr6.3 CDS 13972375 13972522 13972375 ID=cds.MS.gene76035.t1;Parent=MS.gene76035.t1
chr6.3 exon 13973067 13973157 13973067 ID=MS.gene76035.t1.exon3;Parent=MS.gene76035.t1
chr6.3 CDS 13973067 13973157 13973067 ID=cds.MS.gene76035.t1;Parent=MS.gene76035.t1
Gene Sequence

>MS.gene76035

ATGGGTGGTTACATTCAAGAAGCACGCGAGAATCACGTGAAGAAGAAAGTCGAAGAAGGTACCATAATAATACTAAACCCTAACCCAAAACCCATTTTTTCTTCATCATAAAGTTTCAATTTTTATGAACAAAAATGGGTTTATTTCAAATGGGTATTTTTTTATATACATTATTCCATAAATGGGTTTCAAATTATGATCAAATGATTTTATAGTTAAATTGATGTGGTGGGCTATCATCATTTTCATTCAATTGGGTTTTAGTCCCTGTTTGGATAAATGTCTTTTTCTGTAGCTTATAACACAAACAGTTACCATGATAAGCACTTATGTATAAGCTATTTTTATAGTAAAAGATAAAATAAAGTTAAATTGTCTTCGTATAAGCTGTTTTCATAAGCTATCTTGGAGAAATTATGAAAATAAGCTAAAACAGTTTATAAACAATGTTAGTATGTTAAAAGCTGTTTTTACATGTTCTTCCAAACAGTAGATAAGCTCAGATAAGTCAATGCAAACATACCCTTATTCATTTGAATAATATGCTCCTTAGCACCGACACTTTTGATAGAAGGCGTGACAGTGTTCGACTCTGACACATGTGATTACATTCAGCTATGTTACGGTGTTGCTGTGTCAATGTCGTGTCTGGTGTCTGTGTCGGTGCTTCATAGATAAAATGTTCATTCTAAAGTTTTCATTTTTCGTTGTGTTTTTGGAAGAAGAGATGGGTTTTATTCTAATGAGGTTCAAATTTATGATCAAAACTTTGTTTAATGGTGATATTGGAATGATGGGCTATGATCAATTTTGCAGCTTTACGGAGTAAAATGAAGGTGAAGGCACTTAAAGAATGTGATTTTTACACAAAGAAATATGCTGAGTGTGCTATGGGGAGAACACTTTCTGTTGTTTGGCAATGTCGGAAAGTAGCTAAAGAGTTGAATAATTGTCTTCATCAATTGTAAGCTGTTTTATGAAATAGTACTAGAATTATTCTTTGATTTTTTTGTTTTATTTTGGGTTAAGAATTGGAGAAACATACGTAAATGCATCTTCCCTTTGTCGGTTTGGTTATTGAATGTGTCTTTTGTTTGTTGGTTTGGTCCTTGAATGTGTATCCGGTTAGTCAAAGGGAGACCCATAAAATTACTAACGAAAGACACGTATAGGGAGGCTTTTGTAATTTCGATGCATTCGGCGACTATAGTGACACATCTCACACATTTAGGAAATTGGGGTTTAGTCAAAGGGAGAAACTTTCCCCACCTCAAATGACTTCAAAATCTGGGGGAAAAAAACACAAATTTTGTGCAGTTCCAAAGTACACTTCTGGAAACACGAGTTTTTTTAGAAATGTGGCCGTGAAAAGTAGCCTCCCAAGTCAAATCCATCTCTTCAGATTTTACAGTGCCGGCACACTACACGTTAAGTTCATGCCTAAAGCTAGGTGAGGCCTTAGAGTATACAATTGATGGAAGGGGTATTATATGTGCAATTTTTGTTAGATATCAACATGGAGGTGCGACTATAAAATTAGCAGGCTGTGTAGGGGATGGAGCAGGTCGGGCTAGGGGTGTAAAGCAAAGGCTTTACTAG

Protein sequence

>MS.gene76035.t1

MGGYIQEARENHVKKKVEEALRSKMKVKALKECDFYTKKYAECAMGRTLSVVWQCRKVAKELNNCLHQLYQHGGATIKLAGCVGDGAGRARGVKQRLY