AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77785


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77785.t1 MTR_8g045300 96.815 157 5 0 1 157 1 157 1.48e-111 313
MS.gene77785.t1 MTR_8g045520 75.641 156 38 0 1 156 1 156 4.01e-86 249
MS.gene77785.t1 MTR_8g045490 70.513 156 45 1 1 156 1 155 1.16e-82 240
MS.gene77785.t1 MTR_8g045570 71.523 151 43 0 1 151 1 151 1.39e-81 238
MS.gene77785.t1 MTR_8g045400 69.231 156 47 1 1 156 1 155 3.86e-79 231
MS.gene77785.t1 MTR_8g045555 68.874 151 47 0 1 151 1 151 7.94e-78 228
MS.gene77785.t1 MTR_8g045695 66.026 156 52 1 1 156 1 155 7.12e-77 226
MS.gene77785.t1 MTR_8g045735 66.452 155 51 1 1 155 1 154 8.10e-75 221
MS.gene77785.t1 MTR_8g045640 63.462 156 56 1 1 156 1 155 4.64e-71 211
MS.gene77785.t1 MTR_8g045665 63.462 156 56 1 1 156 1 155 7.94e-71 210
MS.gene77785.t1 MTR_8g045560 62.821 156 57 1 1 156 1 155 4.63e-70 208
MS.gene77785.t1 MTR_1g030810 42.208 154 86 2 1 152 1 153 8.29e-38 127
MS.gene77785.t1 MTR_1g030820 39.610 154 90 2 1 152 1 153 3.24e-36 122
MS.gene77785.t1 MTR_8g012550 32.667 150 98 3 6 154 8 155 6.61e-26 96.7
MS.gene77785.t1 MTR_1g030840 32.692 156 97 4 1 149 1 155 1.50e-21 85.5
MS.gene77785.t1 MTR_1g031640 34.694 147 87 5 7 151 7 146 8.10e-21 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77785.t1 AT5G28010 32.895 152 99 3 3 152 15 165 1.21e-22 88.6
MS.gene77785.t1 AT5G28010 32.895 152 99 3 3 152 15 165 1.21e-22 88.6
MS.gene77785.t1 AT1G70830 32.237 152 100 3 3 152 22 172 7.23e-22 86.7
MS.gene77785.t1 AT1G70830 32.237 152 100 3 3 152 22 172 1.50e-21 86.7
MS.gene77785.t1 AT1G70840 31.579 152 101 3 3 152 20 170 4.26e-21 84.7
MS.gene77785.t1 AT1G70830 32.237 152 100 3 3 152 22 172 4.30e-21 86.7
MS.gene77785.t1 AT1G70830 32.237 152 100 3 3 152 22 172 1.25e-20 86.7
MS.gene77785.t1 AT1G70830 30.921 152 102 3 3 152 184 334 3.91e-19 82.4
MS.gene77785.t1 AT1G70890 30.921 152 102 3 3 152 7 157 2.25e-20 82.4
MS.gene77785.t1 AT1G70830 30.921 152 102 3 3 152 137 287 1.85e-19 82.8
MS.gene77785.t1 AT1G70850 30.667 150 101 3 3 150 9 157 4.25e-19 80.9
MS.gene77785.t1 AT1G70850 30.667 150 101 3 3 150 9 157 1.01e-18 81.3
MS.gene77785.t1 AT1G70850 30.986 142 95 3 13 152 175 315 1.31e-17 78.2
MS.gene77785.t1 AT1G70850 30.667 150 101 3 3 150 9 157 1.01e-18 81.3
MS.gene77785.t1 AT1G70850 30.986 142 95 3 13 152 175 315 1.31e-17 78.2
MS.gene77785.t1 AT1G23120 28.105 153 105 3 1 153 1 148 1.93e-18 77.0
MS.gene77785.t1 AT1G23120 28.758 153 102 4 3 153 51 198 1.13e-17 76.3
MS.gene77785.t1 AT1G23130 27.152 151 108 2 3 152 10 159 5.89e-17 73.6
MS.gene77785.t1 AT5G28000 28.947 152 105 3 3 152 13 163 1.64e-16 72.4
MS.gene77785.t1 AT1G70870 32.450 151 89 4 1 151 1 138 1.68e-16 72.0
MS.gene77785.t1 AT4G23670 29.605 152 104 3 1 151 1 150 5.89e-16 70.9
MS.gene77785.t1 AT1G70880 25.658 152 110 3 3 152 8 158 3.15e-15 68.9
MS.gene77785.t1 AT1G24020 29.870 154 104 3 1 152 1 152 4.72e-15 68.6
MS.gene77785.t1 AT1G24020 29.870 154 104 3 1 152 1 152 4.72e-15 68.6
MS.gene77785.t1 AT2G01520 27.632 152 107 3 1 151 1 150 1.38e-14 67.4
MS.gene77785.t1 AT1G14930 26.974 152 108 3 1 151 1 150 6.63e-14 65.5
MS.gene77785.t1 AT2G01530 28.289 152 106 3 1 151 1 150 1.68e-13 64.3
MS.gene77785.t1 AT1G35260 27.517 149 98 4 7 150 6 149 6.35e-13 62.8
MS.gene77785.t1 AT1G35310 26.761 142 101 3 13 153 12 151 9.69e-13 62.4
MS.gene77785.t1 AT4G14060 26.974 152 108 3 1 151 1 150 1.25e-12 62.0
MS.gene77785.t1 AT4G23680 25.658 152 110 3 1 151 1 150 2.55e-12 61.2
MS.gene77785.t1 AT1G30990 27.815 151 105 4 1 150 1 148 1.19e-11 59.7
MS.gene77785.t1 AT1G14940 26.490 151 108 3 1 150 1 149 1.41e-11 59.3

Find 21 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAAACTTAGCACTGAAAT+TGG 0.239596 8.1:+60161215 MS.gene77785:CDS
AGGTATTCCATCCAACTATT+TGG 0.260874 8.1:-60162604 None:intergenic
ATGGCACTACTGCAGTTAAA+TGG 0.307509 8.1:+60162542 MS.gene77785:CDS
TTCATCACCTTGATGCAGCT+TGG 0.437287 8.1:-60161324 None:intergenic
GTAAACTTAGCACTGAAATT+GGG 0.446714 8.1:+60161216 MS.gene77785:CDS
AGAAATTGATGAGCACTATA+AGG 0.468901 8.1:+60162483 MS.gene77785:CDS
AAAATCGTCCACAAGATCTT+CGG 0.479675 8.1:+60162457 MS.gene77785:CDS
TATTCCATCCAACTATTTGG+AGG 0.505271 8.1:-60162601 None:intergenic
AGCTGCATCAAGGTGATGAA+TGG 0.510022 8.1:+60161327 MS.gene77785:CDS
TTTCTTCACCGAAGATCTTG+TGG 0.512241 8.1:-60162465 None:intergenic
CATCATTGAAGTGATTGATA+AGG 0.521648 8.1:+60162516 MS.gene77785:CDS
ATGTTGAACCTCCAAATAGT+TGG 0.544310 8.1:+60162593 MS.gene77785:CDS
CGATTTCACAACAGAGGGCA+AGG 0.552186 8.1:+60162387 MS.gene77785:intron
CATGAAACCAAGCTGCATCA+AGG 0.562710 8.1:+60161317 MS.gene77785:CDS
TGAACCTCCAAATAGTTGGA+TGG 0.602941 8.1:+60162597 MS.gene77785:CDS
TGTCTCGAGTGCATTTGCTA+AGG 0.617854 8.1:-60162624 None:intergenic
ACTCTGATTCAGTTAAACAC+TGG 0.620261 8.1:+60161354 MS.gene77785:CDS
TAGTTTGAAGAACTTGTCAG+AGG 0.640528 8.1:-60161249 None:intergenic
TCATCTTGTCAAGCAAGCAA+AGG 0.651550 8.1:+60162654 MS.gene77785:CDS
GAAGTGATTGATAAGGCTGA+TGG 0.663192 8.1:+60162523 MS.gene77785:CDS
AAACACTGGACTCATGTCCG+AGG 0.721608 8.1:+60161368 MS.gene77785:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCCAATAAAAATTAAAA+CGG - chr8.1:60162358-60162377 None:intergenic 10.0%
!!! CTTTTTTTTTAAGAAAAAAT+TGG + chr8.1:60162262-60162281 MS.gene77785:intron 10.0%
!!! TTGTTTACAATTTTTTTTTT+GGG + chr8.1:60162196-60162215 MS.gene77785:intron 10.0%
!!! TTTGTTTACAATTTTTTTTT+TGG + chr8.1:60162195-60162214 MS.gene77785:intron 10.0%
!! AATAACATATTGCAAAATTT+GGG + chr8.1:60161607-60161626 MS.gene77785:intron 15.0%
!! AGTATAATTATAGAAACATA+TGG - chr8.1:60162291-60162310 None:intergenic 15.0%
!! ATCATCATGATATATAAAAT+TGG + chr8.1:60162083-60162102 MS.gene77785:intron 15.0%
!! ATTAGAAATATAATTTACCT+CGG - chr8.1:60161388-60161407 None:intergenic 15.0%
!!! AGATGAATATTTTAATTGAA+CGG + chr8.1:60161966-60161985 MS.gene77785:intron 15.0%
!!! ATAACCGTTTTAATTTTTAT+TGG + chr8.1:60162351-60162370 MS.gene77785:intron 15.0%
!!! ATAATTTAAAACTATTTGGT+TGG - chr8.1:60162155-60162174 None:intergenic 15.0%
!!! GTCATCTAATTTTAATTTAA+TGG - chr8.1:60161877-60161896 None:intergenic 15.0%
!!! TGTTTACAATTTTTTTTTTG+GGG + chr8.1:60162197-60162216 MS.gene77785:intron 15.0%
!! AACCAATCAACAATTAATAA+TGG + chr8.1:60161797-60161816 MS.gene77785:intron 20.0%
!! ATTCAAGATCAATATCAATA+TGG - chr8.1:60161739-60161758 None:intergenic 20.0%
!! CAATAACATATTGCAAAATT+TGG + chr8.1:60161606-60161625 MS.gene77785:intron 20.0%
!! GATCTAAGTAAAAGATATAA+AGG + chr8.1:60161694-60161713 MS.gene77785:intron 20.0%
!! TAAAAATATGACAGATCTAA+AGG + chr8.1:60161658-60161677 MS.gene77785:intron 20.0%
!! TTCCATTATTAATTGTTGAT+TGG - chr8.1:60161802-60161821 None:intergenic 20.0%
!!! GACATCTTTAAATTTCATTT+TGG + chr8.1:60161537-60161556 MS.gene77785:intron 20.0%
!!! TATAAAATTGGTTGATTTGA+TGG + chr8.1:60162095-60162114 MS.gene77785:intron 20.0%
!!! TTTAGATCTGTCATATTTTT+AGG - chr8.1:60161659-60161678 None:intergenic 20.0%
!!! TTTTAATTTAATGGTCAGTA+AGG - chr8.1:60161868-60161887 None:intergenic 20.0%
! ATGTACAAGTACAACTATAA+AGG + chr8.1:60161995-60162014 MS.gene77785:intron 25.0%
! ATTTCTAATAACACTAACAC+TGG + chr8.1:60161399-60161418 MS.gene77785:intron 25.0%
!! ACTGGATTTATGTTCTATTT+TGG + chr8.1:60161417-60161436 MS.gene77785:intron 25.0%
!!! TTGTCTTCTGATTTTTAACT+TGG + chr8.1:60161464-60161483 MS.gene77785:intron 25.0%
AGAAATTGATGAGCACTATA+AGG + chr8.1:60162483-60162502 MS.gene77785:CDS 30.0%
CATCATTGAAGTGATTGATA+AGG + chr8.1:60162516-60162535 MS.gene77785:CDS 30.0%
! GTAAACTTAGCACTGAAATT+GGG + chr8.1:60161216-60161235 MS.gene77785:CDS 30.0%
! TTGATTTGATGGTTGGAATA+GGG + chr8.1:60162106-60162125 MS.gene77785:intron 30.0%
!! AAACTATTTGGTTGGCTTTA+AGG - chr8.1:60162147-60162166 None:intergenic 30.0%
!! AAATTGGTTGATTTGATGGT+TGG + chr8.1:60162099-60162118 MS.gene77785:intron 30.0%
!! GCTTTAAGGATCTCTAATTT+AGG - chr8.1:60162133-60162152 None:intergenic 30.0%
AAAATCGTCCACAAGATCTT+CGG + chr8.1:60162457-60162476 MS.gene77785:CDS 35.0%
ACAAGATATAGCAAAAGCGA+TGG + chr8.1:60161821-60161840 MS.gene77785:intron 35.0%
ACTCTGATTCAGTTAAACAC+TGG + chr8.1:60161354-60161373 MS.gene77785:CDS 35.0%
AGGTATTCCATCCAACTATT+TGG - chr8.1:60162607-60162626 None:intergenic 35.0%
ATGTTGAACCTCCAAATAGT+TGG + chr8.1:60162593-60162612 MS.gene77785:CDS 35.0%
TAGTTTGAAGAACTTGTCAG+AGG - chr8.1:60161252-60161271 None:intergenic 35.0%
TATTCCATCCAACTATTTGG+AGG - chr8.1:60162604-60162623 None:intergenic 35.0%
! ATAGCAAAAGCGATGGATTT+AGG + chr8.1:60161828-60161847 MS.gene77785:intron 35.0%
! GGTAAACTTAGCACTGAAAT+TGG + chr8.1:60161215-60161234 MS.gene77785:CDS 35.0%
! TGATTTGATGGTTGGAATAG+GGG + chr8.1:60162107-60162126 MS.gene77785:intron 35.0%
!! GTTGATTTGATGGTTGGAAT+AGG + chr8.1:60162105-60162124 MS.gene77785:intron 35.0%
ACCTTCGATTTCACAACAGA+GGG + chr8.1:60162382-60162401 MS.gene77785:intron 40.0%
ATGGCACTACTGCAGTTAAA+TGG + chr8.1:60162542-60162561 MS.gene77785:CDS 40.0%
GAAGTGATTGATAAGGCTGA+TGG + chr8.1:60162523-60162542 MS.gene77785:CDS 40.0%
TACCTTCGATTTCACAACAG+AGG + chr8.1:60162381-60162400 MS.gene77785:intron 40.0%
TCATCTTGTCAAGCAAGCAA+AGG + chr8.1:60162654-60162673 MS.gene77785:CDS 40.0%
TGAACCTCCAAATAGTTGGA+TGG + chr8.1:60162597-60162616 MS.gene77785:CDS 40.0%
TTTCTTCACCGAAGATCTTG+TGG - chr8.1:60162468-60162487 None:intergenic 40.0%
AGCTGCATCAAGGTGATGAA+TGG + chr8.1:60161327-60161346 MS.gene77785:CDS 45.0%
CATGAAACCAAGCTGCATCA+AGG + chr8.1:60161317-60161336 MS.gene77785:CDS 45.0%
TTCATCACCTTGATGCAGCT+TGG - chr8.1:60161327-60161346 None:intergenic 45.0%
! TGTCTCGAGTGCATTTGCTA+AGG - chr8.1:60162627-60162646 None:intergenic 45.0%
!!! AATAATAATTTAAAACTATT+TGG - chr8.1:60162159-60162178 None:intergenic 5.0%
!!! TTTTTTTTTAAGAAAAAATT+GGG + chr8.1:60162263-60162282 MS.gene77785:intron 5.0%
AAACACTGGACTCATGTCCG+AGG + chr8.1:60161368-60161387 MS.gene77785:CDS 50.0%
CGATTTCACAACAGAGGGCA+AGG + chr8.1:60162387-60162406 MS.gene77785:intron 50.0%
GCCCTCTGTTGTGAAATCGA+AGG - chr8.1:60162386-60162405 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 60161203 60162687 60161203 ID=MS.gene77785
chr8.1 mRNA 60161203 60162687 60161203 ID=MS.gene77785.t1;Parent=MS.gene77785
chr8.1 exon 60161203 60161389 60161203 ID=MS.gene77785.t1.exon1;Parent=MS.gene77785.t1
chr8.1 CDS 60161203 60161389 60161203 ID=cds.MS.gene77785.t1;Parent=MS.gene77785.t1
chr8.1 exon 60162401 60162687 60162401 ID=MS.gene77785.t1.exon2;Parent=MS.gene77785.t1
chr8.1 CDS 60162401 60162687 60162401 ID=cds.MS.gene77785.t1;Parent=MS.gene77785.t1
Gene Sequence

>MS.gene77785

ATGGTGCTTGCTGGTAAACTTAGCACTGAAATTGGGATCAAAGCATCCTCTGACAAGTTCTTCAAACTATTCGCATCAAATATTCACGAACTGCAAAACATTTGTGAAAGTGTTCATGAAACCAAGCTGCATCAAGGTGATGAATGGCATCACTCTGATTCAGTTAAACACTGGACTCATGTCCGAGGTAAATTATATTTCTAATAACACTAACACTGGATTTATGTTCTATTTTGGTCTATCAATAAATTTATAATTTATTTGTCTTCTGATTTTTAACTTGGCTAAAGAAAATTTATTTCTCATATTCTTCGAAAAAAATATATTTCTCATAGACATCTTTAAATTTCATTTTGGCTATAACATATTATAAAAAGTTTAATATAACGTAAAAAATAGATGACAATAACATATTGCAAAATTTGGGATATAATTTTTAAAAGAAGATTTAGTCCTAAAAATATGACAGATCTAAAGGTTATATTATGACAGATCTAAGTAAAAGATATAAAGGTTCAAATTGTATAAATATACCATATTGATATTGATCTTGAATTTTGTATAAAACAAACATTTCTCAAGATTTGTTAACAAAACCAATCAACAATTAATAATGGAACAAGATATAGCAAAAGCGATGGATTTAGGCTTCAAATAGATGTCCTTACTGACCATTAAATTAAAATTAGATGACTCACGTATCAATTGAAGAGTTATTGTCAACATACACAATTAATTAAACTCTGAGTCGTTTGATCAAAATAGATGAATATTTTAATTGAACGGTTTGTTATGTACAAGTACAACTATAAAGGATTCTTCTTATACTATAGAAACATAATACATACAGTACATTATATGTCAAAATCAATGATAAATAATCATCATGATATATAAAATTGGTTGATTTGATGGTTGGAATAGGGGCCTAAATTAGAGATCCTTAAAGCCAACCAAATAGTTTTAAATTATTATTATTCATTCAGTTCGATTTTGTTTACAATTTTTTTTTTGGGGAACAACATATTTTATTTAAGAAATGAAAACAATATATTATTACTTTTTTTTTAAGAAAAAATTGGGTTCCATATGTTTCTATAATTATACTTTCATAATTATTCTTTCAAAAAAATGTTTATATAATTATTATAACCGTTTTAATTTTTATTGGAATTAATTACCTTCGATTTCACAACAGAGGGCAAGGTATACACATGTCATGAGAGTTTCGAAGAAATTGATGAAAAGAACAAAAAAATCGTCCACAAGATCTTCGGTGAAGAAATTGATGAGCACTATAAGGTCTTTAAGCTCATCATTGAAGTGATTGATAAGGCTGATGGCACTACTGCAGTTAAATGGACTGTTGAATATGAGAAGATCAATGAAGATGTTGAACCTCCAAATAGTTGGATGGAATACCTTAGCAAATGCACTCGAGACATTGATGCTCATCTTGTCAAGCAAGCAAAGGTTGCTCCATAA

Protein sequence

>MS.gene77785.t1

MVLAGKLSTEIGIKASSDKFFKLFASNIHELQNICESVHETKLHQGDEWHHSDSVKHWTHVREGKVYTCHESFEEIDEKNKKIVHKIFGEEIDEHYKVFKLIIEVIDKADGTTAVKWTVEYEKINEDVEPPNSWMEYLSKCTRDIDAHLVKQAKVAP