AlfalfaGEDB | MS.gene78659

M. sativa cultivar XinJiangDaYe / MS.gene78659

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78659.t1	XP_003628087.1	98.3	60	1	0	1	60	1	60	5.70E-24	119.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78659.t1	G7LJ77	98.3	60	1	0	1	60	1	60	4.1e-24	119.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene78659	0.802992	4.31E-49	-1.69E-46
MS.gene049341	MS.gene78659	0.808918	2.45E-50	-1.69E-46
MS.gene049501	MS.gene78659	0.811278	7.59E-51	-1.69E-46
MS.gene049756	MS.gene78659	0.810351	1.20E-50	-1.69E-46
MS.gene050277	MS.gene78659	0.812411	4.30E-51	-1.69E-46
MS.gene050303	MS.gene78659	0.840948	6.39E-58	-1.69E-46
MS.gene050438	MS.gene78659	0.847733	9.59E-60	-1.69E-46
MS.gene050719	MS.gene78659	0.851727	7.35E-61	-1.69E-46
MS.gene050721	MS.gene78659	0.850047	2.19E-60	-1.69E-46
MS.gene050724	MS.gene78659	0.847617	1.03E-59	-1.69E-46
MS.gene050807	MS.gene78659	0.80491	1.72E-49	-1.69E-46
MS.gene050815	MS.gene78659	0.877828	4.59E-69	-1.69E-46
MS.gene050824	MS.gene78659	0.814764	1.30E-51	-1.69E-46
MS.gene051088	MS.gene78659	0.810798	9.63E-51	-1.69E-46
MS.gene051243	MS.gene78659	0.832008	1.21E-55	-1.69E-46
MS.gene051328	MS.gene78659	0.828209	1.02E-54	-1.69E-46
MS.gene051349	MS.gene78659	0.813116	3.01E-51	-1.69E-46
MS.gene05134	MS.gene78659	0.811883	5.60E-51	-1.69E-46
MS.gene051577	MS.gene78659	0.815039	1.13E-51	-1.69E-46
MS.gene051757	MS.gene78659	0.815197	1.05E-51	-1.69E-46
MS.gene052012	MS.gene78659	0.817094	3.94E-52	-1.69E-46
MS.gene052302	MS.gene78659	0.824743	6.84E-54	-1.69E-46
MS.gene052376	MS.gene78659	0.839666	1.38E-57	-1.69E-46
MS.gene052896	MS.gene78659	0.811901	5.55E-51	-1.69E-46
MS.gene053008	MS.gene78659	0.827713	1.34E-54	-1.69E-46
MS.gene053054	MS.gene78659	-0.808898	2.47E-50	-1.69E-46
MS.gene053074	MS.gene78659	0.816438	5.53E-52	-1.69E-46
MS.gene054472	MS.gene78659	0.824014	1.02E-53	-1.69E-46
MS.gene055512	MS.gene78659	0.807939	3.96E-50	-1.69E-46
MS.gene055623	MS.gene78659	0.812432	4.25E-51	-1.69E-46
MS.gene055818	MS.gene78659	0.804716	1.89E-49	-1.69E-46
MS.gene056631	MS.gene78659	0.832301	1.02E-55	-1.69E-46
MS.gene056760	MS.gene78659	0.840803	6.98E-58	-1.69E-46
MS.gene057060	MS.gene78659	0.804874	1.75E-49	-1.69E-46
MS.gene057140	MS.gene78659	0.83765	4.59E-57	-1.69E-46
MS.gene057260	MS.gene78659	0.812573	3.96E-51	-1.69E-46
MS.gene058084	MS.gene78659	0.833132	6.36E-56	-1.69E-46
MS.gene058146	MS.gene78659	0.807439	5.05E-50	-1.69E-46
MS.gene058168	MS.gene78659	0.825928	3.59E-54	-1.69E-46
MS.gene058182	MS.gene78659	0.812127	4.96E-51	-1.69E-46
MS.gene058906	MS.gene78659	0.879959	8.14E-70	-1.69E-46
MS.gene058982	MS.gene78659	0.801307	9.58E-49	-1.69E-46
MS.gene059151	MS.gene78659	0.808997	2.35E-50	-1.69E-46
MS.gene059249	MS.gene78659	0.836653	8.25E-57	-1.69E-46
MS.gene05954	MS.gene78659	0.828948	6.77E-55	-1.69E-46
MS.gene059569	MS.gene78659	0.811281	7.57E-51	-1.69E-46
MS.gene059898	MS.gene78659	0.801542	8.57E-49	-1.69E-46
MS.gene06001	MS.gene78659	0.828342	9.48E-55	-1.69E-46
MS.gene060322	MS.gene78659	0.821812	3.31E-53	-1.69E-46
MS.gene060429	MS.gene78659	0.836725	7.91E-57	-1.69E-46
MS.gene060572	MS.gene78659	0.828672	7.89E-55	-1.69E-46
MS.gene060619	MS.gene78659	0.800906	1.16E-48	-1.69E-46
MS.gene060803	MS.gene78659	0.825302	5.05E-54	-1.69E-46
MS.gene060907	MS.gene78659	0.825534	4.45E-54	-1.69E-46
MS.gene060998	MS.gene78659	0.809843	1.55E-50	-1.69E-46
MS.gene06114	MS.gene78659	0.809434	1.90E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78659.t1	MTR_8g043400	98.333	60	1	0	1	60	1	60	3.07e-38	120

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene78659.t1	AT3G15395	65.000	60	20	1	1	60	1	59	3.54e-24	85.5
MS.gene78659.t1	AT3G15395	65.000	60	20	1	1	60	1	59	3.54e-24	85.5
MS.gene78659.t1	AT3G15395	65.000	60	20	1	1	60	1	59	3.54e-24	85.5
MS.gene78659.t1	AT3G15395	65.000	60	20	1	1	60	1	59	3.54e-24	85.5
MS.gene78659.t1	AT3G15395	65.000	60	20	1	1	60	1	59	3.54e-24	85.5

Find 20 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTCTTTGGGCTTGTGCTAT+TGG	0.281471	8.4:-56542389	MS.gene78659:CDS
AGTGGTCTATGAGAGTTCTT+TGG	0.313525	8.4:-56542403	MS.gene78659:CDS
AGGCTTGAAAGGAATGGATT+TGG	0.349048	8.4:-56541965	MS.gene78659:CDS
ATCCATTCCTTTCAAGCCTT+CGG	0.357129	8.4:+56541969	None:intergenic
TAGAAAGACAAAAGCAGAAT+AGG	0.394330	8.4:-56542005	MS.gene78659:CDS
GTGGTCTATGAGAGTTCTTT+GGG	0.402171	8.4:-56542402	MS.gene78659:CDS
TACCTGTCTAGGTCTGTATA+TGG	0.414586	8.4:-56542034	MS.gene78659:intron
CTTGTCCGTAATTACTCCTC+TGG	0.492027	8.4:+56542424	None:intergenic
GGGAACCAGAGGAGTAATTA+CGG	0.516023	8.4:-56542429	MS.gene78659:CDS
CTTGAAAGGAATGGATTTGG+AGG	0.518401	8.4:-56541962	MS.gene78659:CDS
AAAGGAATGGATTTGGAGGA+AGG	0.535893	8.4:-56541958	MS.gene78659:CDS
GGCCGAAGGCTTGAAAGGAA+TGG	0.548731	8.4:-56541971	MS.gene78659:CDS
ATGTTGGCCGAAGGCTTGAA+AGG	0.560678	8.4:-56541976	MS.gene78659:CDS
GCAGAATAGGCAGAGGATGT+TGG	0.565240	8.4:-56541992	MS.gene78659:CDS
ATGGATTTGGAGGAAGGCAA+TGG	0.569370	8.4:-56541952	MS.gene78659:CDS
AATTACTCCTCTGGTTCCCA+TGG	0.583145	8.4:+56542433	None:intergenic
GACAAAAGCAGAATAGGCAG+AGG	0.586343	8.4:-56541999	MS.gene78659:CDS
CTGCATGCCATGGGAACCAG+AGG	0.620238	8.4:-56542440	None:intergenic
GAGGAGTAATTACGGACAAG+TGG	0.636089	8.4:-56542421	MS.gene78659:CDS
AGGCAGAGGATGTTGGCCGA+AGG	0.646645	8.4:-56541985	MS.gene78659:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TACATTTAAGTTTGTTAACA+AGG	+	chr8.4:56542063-56542082	None:intergenic	20.0%
!	AAAGAAGGTTCCATTAATAA+AGG	+	chr8.4:56542033-56542052	None:intergenic	25.0%
!	AAGAAGGTTCCATTAATAAA+GGG	+	chr8.4:56542032-56542051	None:intergenic	25.0%
!!	GGTAAACTATTTTCAAATAG+CGG	-	chr8.4:56542242-56542261	MS.gene78659:intron	25.0%
!!	GTTTTACGATACGTTATTTA+GGG	-	chr8.4:56542221-56542240	MS.gene78659:intron	25.0%
!!	TGTTTTACGATACGTTATTT+AGG	-	chr8.4:56542220-56542239	MS.gene78659:intron	25.0%
	CATTCTAGACCCTTTATTAA+TGG	-	chr8.4:56542020-56542039	MS.gene78659:CDS	30.0%
	TAACAGCTATCTAACAACAT+CGG	+	chr8.4:56542170-56542189	None:intergenic	30.0%
	TAGAAAGACAAAAGCAGAAT+AGG	-	chr8.4:56542365-56542384	MS.gene78659:intron	30.0%
!!	AAGTTTGTTAACAAGGCTTC+AGG	+	chr8.4:56542056-56542075	None:intergenic	35.0%
!!!	ACTATAGCACTTTTTCGTAG+TGG	-	chr8.4:56542275-56542294	MS.gene78659:intron	35.0%
	AACCATATACAGACCTAGAC+AGG	+	chr8.4:56542341-56542360	None:intergenic	40.0%
	ACACTGTACTATACCTGTCT+AGG	-	chr8.4:56542325-56542344	MS.gene78659:intron	40.0%
	AGATAGCTGTTATAGCGTAG+TGG	-	chr8.4:56542178-56542197	MS.gene78659:intron	40.0%
	AGTGGTCTATGAGAGTTCTT+TGG	-	chr8.4:56541967-56541986	MS.gene78659:CDS	40.0%
	ATCCATTCCTTTCAAGCCTT+CGG	+	chr8.4:56542404-56542423	None:intergenic	40.0%
	GTGGTCTATGAGAGTTCTTT+GGG	-	chr8.4:56541968-56541987	MS.gene78659:CDS	40.0%
	TAACAAGGCTTCAGGAAAGA+AGG	+	chr8.4:56542048-56542067	None:intergenic	40.0%
	TACCTGTCTAGGTCTGTATA+TGG	-	chr8.4:56542336-56542355	MS.gene78659:intron	40.0%
!	AAAGGAATGGATTTGGAGGA+AGG	-	chr8.4:56542412-56542431	MS.gene78659:CDS	40.0%
!	AGGCTTGAAAGGAATGGATT+TGG	-	chr8.4:56542405-56542424	MS.gene78659:CDS	40.0%
!	CTTGAAAGGAATGGATTTGG+AGG	-	chr8.4:56542408-56542427	MS.gene78659:CDS	40.0%
	ATGGATTTGGAGGAAGGCAA+TGG	-	chr8.4:56542418-56542437	MS.gene78659:CDS	45.0%
	CTTGTCCGTAATTACTCCTC+TGG	+	chr8.4:56541949-56541968	None:intergenic	45.0%
	GACAAAAGCAGAATAGGCAG+AGG	-	chr8.4:56542371-56542390	MS.gene78659:intron	45.0%
	GAGGAGTAATTACGGACAAG+TGG	-	chr8.4:56541949-56541968	MS.gene78659:CDS	45.0%
	GGGAACCAGAGGAGTAATTA+CGG	-	chr8.4:56541941-56541960	MS.gene78659:CDS	45.0%
	GTTCTTTGGGCTTGTGCTAT+TGG	-	chr8.4:56541981-56542000	MS.gene78659:CDS	45.0%
	ATGTTGGCCGAAGGCTTGAA+AGG	-	chr8.4:56542394-56542413	MS.gene78659:CDS	50.0%
	GCAGAATAGGCAGAGGATGT+TGG	-	chr8.4:56542378-56542397	MS.gene78659:CDS	50.0%
	GGCCGAAGGCTTGAAAGGAA+TGG	-	chr8.4:56542399-56542418	MS.gene78659:CDS	55.0%
	AGGCAGAGGATGTTGGCCGA+AGG	-	chr8.4:56542385-56542404	MS.gene78659:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	56541939	56542453	56541939	ID=MS.gene78659
chr8.4	mRNA	56541939	56542453	56541939	ID=MS.gene78659.t1;Parent=MS.gene78659
chr8.4	exon	56542378	56542453	56542378	ID=MS.gene78659.t1.exon1;Parent=MS.gene78659.t1
chr8.4	CDS	56542378	56542453	56542378	ID=cds.MS.gene78659.t1;Parent=MS.gene78659.t1
chr8.4	exon	56541939	56542045	56541939	ID=MS.gene78659.t1.exon2;Parent=MS.gene78659.t1
chr8.4	CDS	56541939	56542045	56541939	ID=cds.MS.gene78659.t1;Parent=MS.gene78659.t1

Gene Sequence

>MS.gene78659

ATGGGAACCAGAGGAGTAATTACGGACAAGTGGTCTATGAGAGTTCTTTGGGCTTGTGCTATTGGAAGTGCTGTTAGTAAGCATTCTAGACCCTTTATTAATGGAACCTTCTTTCCTGAAGCCTTGTTAACAAACTTAAATGTAACTTTGTTATGTTATGTCTCTTCACTTTTTGTATAAAACATTGTTCATATGATCATATTTTTCTCATTATGAATCACTACTATACCGATGTTGTTAGATAGCTGTTATAGCGTAGTGGAACTCAAACAAATTGCTGTTGTTTTACGATACGTTATTTAGGGTAAACTATTTTCAAATAGCGGCTATAACAATACTATAGCACTTTTTCGTAGTGGAATTTCAACAACTGATATTTTCTGACGACACTGTACTATACCTGTCTAGGTCTGTATATGGTTGCTGTAGAAAGACAAAAGCAGAATAGGCAGAGGATGTTGGCCGAAGGCTTGAAAGGAATGGATTTGGAGGAAGGCAATGGTGAAAATGTTTGA

Protein sequence

>MS.gene78659.t1

MGTRGVITDKWSMRVLWACAIGSAVSLYMVAVERQKQNRQRMLAEGLKGMDLEEGNGENV