AlfalfaGEDB | MS.gene81545

M. sativa cultivar XinJiangDaYe / MS.gene81545

KEGG: K03232
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0005488 GO:0005575 GO:0005853 GO:0006414 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0032991 GO:0034645 GO:0043170 GO:0044237 GO:0044249 GO:0044260 GO:0045182 GO:0071704 GO:0090079 GO:0097159 GO:1901363 GO:1901576
PFAM: PF00736.22

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81545.t1	RHN74026.1	92.8	251	3	1	1	236	1	251	5.10E-84	320.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81545.t1	O81918	80.6	222	43	0	1	222	1	222	7.0e-58	225.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81545.t1	A0A396J742	92.8	251	3	1	1	236	1	251	3.7e-84	320.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049411	MS.gene81545	0.811039	8.55E-51	-1.69E-46
MS.gene050752	MS.gene81545	0.801628	8.23E-49	-1.69E-46
MS.gene050955	MS.gene81545	0.821364	4.20E-53	-1.69E-46
MS.gene052479	MS.gene81545	0.824972	6.04E-54	-1.69E-46
MS.gene054403	MS.gene81545	0.806263	8.96E-50	-1.69E-46
MS.gene055555	MS.gene81545	0.833312	5.73E-56	-1.69E-46
MS.gene055765	MS.gene81545	0.823268	1.52E-53	-1.69E-46
MS.gene057694	MS.gene81545	0.820171	7.90E-53	-1.69E-46
MS.gene057705	MS.gene81545	0.8268	2.22E-54	-1.69E-46
MS.gene059227	MS.gene81545	0.817561	3.09E-52	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene001098	MS.gene81545	PPI
MS.gene005994	MS.gene81545	PPI
MS.gene005996	MS.gene81545	PPI
MS.gene006000	MS.gene81545	PPI
MS.gene016914	MS.gene81545	PPI
MS.gene036405	MS.gene81545	PPI
MS.gene041771	MS.gene81545	PPI
MS.gene043773	MS.gene81545	PPI
MS.gene057052	MS.gene81545	PPI
MS.gene33103	MS.gene81545	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81545.t1	MTR_2g461200	96.889	225	7	0	1	225	1	225	6.99e-156	432
MS.gene81545.t1	MTR_2g461200	96.889	225	7	0	1	225	1	225	1.19e-155	432
MS.gene81545.t1	MTR_4g019610	92.444	225	17	0	1	225	1	225	1.13e-126	358
MS.gene81545.t1	MTR_4g019610	92.444	225	17	0	1	225	1	225	1.71e-126	359
MS.gene81545.t1	MTR_2g461200	97.790	181	4	0	1	181	1	181	2.22e-124	350
MS.gene81545.t1	MTR_5g088660	53.778	225	96	3	1	225	1	217	2.34e-70	215
MS.gene81545.t1	MTR_5g088685	75.000	48	12	0	133	180	2	49	6.04e-18	75.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81545.t1	AT2G18110	77.273	220	49	1	7	225	6	225	5.08e-101	293
MS.gene81545.t1	AT1G30230	76.364	220	51	1	7	225	6	225	3.01e-97	283
MS.gene81545.t1	AT1G30230	76.364	220	51	1	7	225	6	225	6.63e-97	284
MS.gene81545.t1	AT5G12110	57.205	229	87	3	1	225	1	222	4.21e-70	214
MS.gene81545.t1	AT5G19510	54.667	225	95	2	1	225	1	218	3.70e-62	194

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Find 61 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTGTTCCTGTTGGGTA+CGG	0.517929	2.2:-33477465	MS.gene81545:CDS
AACATGGAGATCATGGTAGG+GGG	0.694332	2.2:-33477194	MS.gene81545:CDS
AATATGGGAATGTTGCTAGG+TGG	0.778608	2.2:-33478634	MS.gene81545:CDS
AATCTGCAATTCGTATAACA+TGG	0.639814	2.2:-33477210	MS.gene81545:CDS
ACATTCCCATATTCATATGA+TGG	0.500557	2.2:+33478644	None:intergenic
ACTCTGGACATACTCATTGA+TGG	0.454635	2.2:+33477356	None:intergenic
AGAAGAAGGCAGCTGAGGAA+CGG	0.514292	2.2:-33478293	MS.gene81545:CDS
AGAGACTGAGGAAGAGAAGA+AGG	0.523236	2.2:-33478307	MS.gene81545:CDS
AGCAGTGAGATCTGTTCAGT+TGG	0.499725	2.2:-33478069	MS.gene81545:CDS
AGTTACACTCTATAACCTTA+AGG	0.486396	2.2:-33478888	MS.gene81545:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATCTGCAATTCGTATAACA+TGG	-	chr2.2:33478858-33478877	MS.gene81545:CDS	30.0%
ACATTCCCATATTCATATGA+TGG	+	chr2.2:33477427-33477446	None:intergenic	30.0%
AGTTACACTCTATAACCTTA+AGG	-	chr2.2:33477180-33477199	MS.gene81545:CDS	30.0%
ATGGTGCACAAATAAGATAT+TGG	-	chr2.2:33478336-33478355	MS.gene81545:CDS	30.0%
CAGTTCCATCATATGAATAT+GGG	-	chr2.2:33477419-33477438	MS.gene81545:CDS	30.0%
CCAGATTCCAAATAATTATG+AGG	+	chr2.2:33477862-33477881	None:intergenic	30.0%
TATAATTGACAAAGATGGCA+AGG	+	chr2.2:33477501-33477520	None:intergenic	30.0%
TCAGTTCCATCATATGAATA+TGG	-	chr2.2:33477418-33477437	MS.gene81545:CDS	30.0%
TCATATCTCACTTGATCTAA+TGG	-	chr2.2:33478218-33478237	MS.gene81545:intron	30.0%
ACGGTGCACAAATAAGATAT+TGG	-	chr2.2:33478148-33478167	MS.gene81545:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	33477175	33478915	33477175	ID=MS.gene81545
chr2.2	mRNA	33477175	33478915	33477175	ID=MS.gene81545.t1;Parent=MS.gene81545
chr2.2	exon	33478830	33478915	33478830	ID=MS.gene81545.t1.exon1;Parent=MS.gene81545.t1
chr2.2	CDS	33478830	33478915	33478830	ID=cds.MS.gene81545.t1;Parent=MS.gene81545.t1
chr2.2	exon	33478602	33478718	33478602	ID=MS.gene81545.t1.exon2;Parent=MS.gene81545.t1
chr2.2	CDS	33478602	33478718	33478602	ID=cds.MS.gene81545.t1;Parent=MS.gene81545.t1
chr2.2	exon	33478256	33478470	33478256	ID=MS.gene81545.t1.exon3;Parent=MS.gene81545.t1
chr2.2	CDS	33478256	33478470	33478256	ID=cds.MS.gene81545.t1;Parent=MS.gene81545.t1
chr2.2	exon	33478048	33478161	33478048	ID=MS.gene81545.t1.exon4;Parent=MS.gene81545.t1
chr2.2	CDS	33478048	33478161	33478048	ID=cds.MS.gene81545.t1;Parent=MS.gene81545.t1
chr2.2	exon	33477369	33477489	33477369	ID=MS.gene81545.t1.exon5;Parent=MS.gene81545.t1
chr2.2	CDS	33477369	33477489	33477369	ID=cds.MS.gene81545.t1;Parent=MS.gene81545.t1
chr2.2	exon	33477175	33477232	33477175	ID=MS.gene81545.t1.exon6;Parent=MS.gene81545.t1
chr2.2	CDS	33477175	33477232	33477175	ID=cds.MS.gene81545.t1;Parent=MS.gene81545.t1

Gene Sequence

>MS.gene81545

ATGGCAGTTACACTCTATAACCTTAAGGCTGAATCTGGGTTGAAGAAGCTTGATGAGTACCTTCTTACACGCAGTTACATCACTGGGTAGGTTTTTTTTTTTTCTTTCTTTCTTTCTTTTCATAATTGATTGTTTATTTATTTGTTTTATTTATTTTTTTGTTTCTTATGAATTCTGTTTTTATTTGATCTGTTTAGGTACCAAGCTTCAAAGGATGACATCACTGTTTATGCAGCTTTGCCATCAGTTCCATCATATGAATATGGGAATGTTGCTAGGTGGTACAAGCACATTGATGCATTGTTGAGAATTGCGTAAGTTCTCCTTGCCATCTTTGTCAATTATATGTGAACATTGTAAGCTGATTAGAAATTAGTAATTCAAATGACTTGTATCCGTTATGCAATTATCTTTTTCAAATTTTAATTGAGTTTTGTGACTGTAGTGGTGTTTCTGGTGAGGGATCTGGTGTCACTGTGGAATCTTCTCTTGTGGCTGAAGAGACTGTTGCCACTCCTCCAGTTTCTGACACAAAGGCCGCTGAAGCTGAGGATGAAGATGATGACGATGTTGATTTGTTTGGTGAAGAGACTGAGGAAGAGAAGAAGGCAGCTGAGGAACGGGCAGCAGCTGTGAAGGCATCTGGCAAAAAGAAAGAGAGTAAGTTTCTGTTGTATTTCATATCCTCATAATTATTTGGAATCTGGTTGGACATCAATTTAGAGTATTGGTTTTCTGCTGATTGCATTATCAGGTGGGAAATCATCTGTATTGTTGGATGTGAAGCCATGGGATGATGAGACCGACATGAAGAAGCTTGAAGAAGCAGTGAGATCTGTTCAGTTGGAAGGGTTGTTTTGGGGTGCATGTATGTTATTTGCTCATTTCAGCTTTGTTGATTGTTTTCATTCTATGATATGATTCTGAATAACAAGTTTCAGTTTATTGTATACTATTTTTCAAGGCTATGTCTACGGTGCACAAATAAGATATTGGTGTACCATGCATATGTTTGTTTTCCACTATTGGGTCATTAAGTCGCTTCATATCTCACTTGATCTAATGGTGGAATGTTTTATCCAATAGTGAAAGCAAACTTGTTCACGGTCATCTCATTGTGCACCATTCAGTTTATTGTATACTATTTTTCAAGGCATGTCTATGGTGCACAAATAAGATATTGGTATTCTATGCATAAGTTTGTTTTCCACTATTGGGTCAATAAGTCCCTTCATATCTCGCTTGATCTAATGGTGGAATGTTTTATCCAATAGCGAAATATCTTATTTTTCAATTGTAAAAGAGAGACTATTTTCAAATGCTCGAATGCGTAAAAATATACATTAGTCTTTGCTATTAATTTTATTTGTTTGTTTTATTTACTAGCTATCTCCATGATTCTGATATTAGTTTATTGCTTTCATAGCCAAACTTGTTCCTGTTGGGTACGGTATCAAGAAACTTCAAATTATGCTTACTATTGTGGATGATCTTGTTTCCGTTGACAATATGGTTGAGGATTATCTTACTGTTGAACCCATCAATGAGTATGTCCAGAGTTGTGACATTGTGGCCTTCAATAAAATCTGTAAGCAATTTTCAGATTTTATTTGTCTGTTGGTTTTATAATTATTTTTCTCATAAACTGCATATCTTAATGCCATATCATATTCTTTGGTGCAGAATCTGCAATTCGTATAACATGGAGATCATGGTAGGGGGCAGAAATTGCAATTATTAG

Protein sequence

>MS.gene81545.t1

MAVTLYNLKAESGLKKLDEYLLTRSYITGYQASKDDITVYAALPSVPSYEYGNVARWYKHIDALLRIAGVSGEGSGVTVESSLVAEETVATPPVSDTKAAEAEDEDDDDVDLFGEETEEEKKAAEERAAAVKASGKKKESGKSSVLLDVKPWDDETDMKKLEEAVRSVQLEGLFWGASKLVPVGYGIKKLQIMLTIVDDLVSVDNMVEDYLTVEPINEICNSYNMEIMVGGRNCNY