Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68663.t1 | RHN74026.1 | 98.4 | 251 | 4 | 0 | 1 | 251 | 1 | 251 | 3.60E-96 | 361.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68663.t1 | O81918 | 82.3 | 231 | 41 | 0 | 1 | 231 | 1 | 231 | 1.7e-65 | 250.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68663.t1 | A0A396J742 | 98.4 | 251 | 4 | 0 | 1 | 251 | 1 | 251 | 2.6e-96 | 361.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene68663 | 0.814129 | 1.80E-51 | -1.69E-46 |
| MS.gene04918 | MS.gene68663 | 0.813685 | 2.26E-51 | -1.69E-46 |
| MS.gene049254 | MS.gene68663 | 0.82353 | 1.32E-53 | -1.69E-46 |
| MS.gene049350 | MS.gene68663 | 0.849173 | 3.83E-60 | -1.69E-46 |
| MS.gene049441 | MS.gene68663 | 0.808426 | 3.12E-50 | -1.69E-46 |
| MS.gene049499 | MS.gene68663 | 0.80264 | 5.10E-49 | -1.69E-46 |
| MS.gene049666 | MS.gene68663 | 0.83178 | 1.37E-55 | -1.69E-46 |
| MS.gene049711 | MS.gene68663 | 0.838259 | 3.20E-57 | -1.69E-46 |
| MS.gene049807 | MS.gene68663 | 0.858815 | 6.36E-63 | -1.69E-46 |
| MS.gene049821 | MS.gene68663 | 0.815893 | 7.32E-52 | -1.69E-46 |
| MS.gene049824 | MS.gene68663 | 0.817323 | 3.50E-52 | -1.69E-46 |
| MS.gene049825 | MS.gene68663 | 0.822365 | 2.46E-53 | -1.69E-46 |
| MS.gene049873 | MS.gene68663 | 0.80666 | 7.39E-50 | -1.69E-46 |
| MS.gene050125 | MS.gene68663 | 0.802641 | 5.09E-49 | -1.69E-46 |
| MS.gene050273 | MS.gene68663 | 0.813133 | 2.99E-51 | -1.69E-46 |
| MS.gene050559 | MS.gene68663 | 0.822226 | 2.65E-53 | -1.69E-46 |
| MS.gene050561 | MS.gene68663 | 0.813736 | 2.20E-51 | -1.69E-46 |
| MS.gene050562 | MS.gene68663 | 0.80955 | 1.79E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene68663 | 0.805186 | 1.51E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene68663 | 0.802387 | 5.75E-49 | -1.69E-46 |
| MS.gene050901 | MS.gene68663 | 0.825323 | 4.99E-54 | -1.69E-46 |
| MS.gene050945 | MS.gene68663 | 0.818654 | 1.75E-52 | -1.69E-46 |
| MS.gene051234 | MS.gene68663 | 0.828986 | 6.63E-55 | -1.69E-46 |
| MS.gene051279 | MS.gene68663 | 0.833342 | 5.64E-56 | -1.69E-46 |
| MS.gene051343 | MS.gene68663 | 0.80248 | 5.50E-49 | -1.69E-46 |
| MS.gene05134 | MS.gene68663 | 0.816808 | 4.57E-52 | -1.69E-46 |
| MS.gene051613 | MS.gene68663 | 0.815324 | 9.80E-52 | -1.69E-46 |
| MS.gene051699 | MS.gene68663 | 0.823304 | 1.49E-53 | -1.69E-46 |
| MS.gene051700 | MS.gene68663 | 0.838154 | 3.40E-57 | -1.69E-46 |
| MS.gene051719 | MS.gene68663 | 0.819176 | 1.33E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene68663 | 0.829579 | 4.76E-55 | -1.69E-46 |
| MS.gene051744 | MS.gene68663 | 0.821832 | 3.27E-53 | -1.69E-46 |
| MS.gene051791 | MS.gene68663 | 0.803362 | 3.61E-49 | -1.69E-46 |
| MS.gene051832 | MS.gene68663 | 0.833136 | 6.34E-56 | -1.69E-46 |
| MS.gene052037 | MS.gene68663 | 0.809117 | 2.22E-50 | -1.69E-46 |
| MS.gene052072 | MS.gene68663 | 0.818232 | 2.18E-52 | -1.69E-46 |
| MS.gene052133 | MS.gene68663 | 0.808673 | 2.76E-50 | -1.69E-46 |
| MS.gene052239 | MS.gene68663 | -0.857992 | 1.12E-62 | -1.69E-46 |
| MS.gene052516 | MS.gene68663 | 0.820026 | 8.53E-53 | -1.69E-46 |
| MS.gene052519 | MS.gene68663 | 0.805517 | 1.28E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene68663 | 0.821879 | 3.19E-53 | -1.69E-46 |
| MS.gene052584 | MS.gene68663 | 0.8361 | 1.14E-56 | -1.69E-46 |
| MS.gene052628 | MS.gene68663 | 0.830665 | 2.58E-55 | -1.69E-46 |
| MS.gene052793 | MS.gene68663 | 0.807328 | 5.33E-50 | -1.69E-46 |
| MS.gene052947 | MS.gene68663 | 0.839957 | 1.16E-57 | -1.69E-46 |
| MS.gene053122 | MS.gene68663 | 0.876804 | 1.04E-68 | -1.69E-46 |
| MS.gene053144 | MS.gene68663 | 0.830705 | 2.53E-55 | -1.69E-46 |
| MS.gene053195 | MS.gene68663 | 0.831721 | 1.42E-55 | -1.69E-46 |
| MS.gene053226 | MS.gene68663 | 0.846 | 2.86E-59 | -1.69E-46 |
| MS.gene053541 | MS.gene68663 | 0.805172 | 1.52E-49 | -1.69E-46 |
| MS.gene053542 | MS.gene68663 | 0.814366 | 1.60E-51 | -1.69E-46 |
| MS.gene053581 | MS.gene68663 | 0.832559 | 8.82E-56 | -1.69E-46 |
| MS.gene053599 | MS.gene68663 | 0.817268 | 3.60E-52 | -1.69E-46 |
| MS.gene05364 | MS.gene68663 | 0.834015 | 3.83E-56 | -1.69E-46 |
| MS.gene054142 | MS.gene68663 | 0.835807 | 1.35E-56 | -1.69E-46 |
| MS.gene054584 | MS.gene68663 | 0.828532 | 8.53E-55 | -1.69E-46 |
| MS.gene05458 | MS.gene68663 | 0.800999 | 1.11E-48 | -1.69E-46 |
| MS.gene054651 | MS.gene68663 | 0.83196 | 1.24E-55 | -1.69E-46 |
| MS.gene054709 | MS.gene68663 | 0.816352 | 5.78E-52 | -1.69E-46 |
| MS.gene055464 | MS.gene68663 | 0.815981 | 7.00E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene68663 | 0.819045 | 1.43E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene68663 | 0.818332 | 2.07E-52 | -1.69E-46 |
| MS.gene055468 | MS.gene68663 | 0.8304 | 3.00E-55 | -1.69E-46 |
| MS.gene055555 | MS.gene68663 | 0.842026 | 3.32E-58 | -1.69E-46 |
| MS.gene055580 | MS.gene68663 | -0.854533 | 1.16E-61 | -1.69E-46 |
| MS.gene055694 | MS.gene68663 | 0.847095 | 1.44E-59 | -1.69E-46 |
| MS.gene055808 | MS.gene68663 | 0.812906 | 3.35E-51 | -1.69E-46 |
| MS.gene056005 | MS.gene68663 | 0.804381 | 2.22E-49 | -1.69E-46 |
| MS.gene056043 | MS.gene68663 | -0.840137 | 1.04E-57 | -1.69E-46 |
| MS.gene056050 | MS.gene68663 | 0.831332 | 1.77E-55 | -1.69E-46 |
| MS.gene056091 | MS.gene68663 | 0.802409 | 5.69E-49 | -1.69E-46 |
| MS.gene056215 | MS.gene68663 | 0.802281 | 6.04E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene68663 | 0.852559 | 4.26E-61 | -1.69E-46 |
| MS.gene056366 | MS.gene68663 | 0.817513 | 3.17E-52 | -1.69E-46 |
| MS.gene056539 | MS.gene68663 | 0.802187 | 6.32E-49 | -1.69E-46 |
| MS.gene056595 | MS.gene68663 | 0.811207 | 7.86E-51 | -1.69E-46 |
| MS.gene056677 | MS.gene68663 | 0.813545 | 2.42E-51 | -1.69E-46 |
| MS.gene056776 | MS.gene68663 | 0.813274 | 2.78E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene68663 | 0.809415 | 1.91E-50 | -1.69E-46 |
| MS.gene05689 | MS.gene68663 | 0.801249 | 9.84E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene68663 | 0.871795 | 5.21E-67 | -1.69E-46 |
| MS.gene057208 | MS.gene68663 | 0.808115 | 3.63E-50 | -1.69E-46 |
| MS.gene057287 | MS.gene68663 | 0.809113 | 2.22E-50 | -1.69E-46 |
| MS.gene057444 | MS.gene68663 | 0.82312 | 1.64E-53 | -1.69E-46 |
| MS.gene057694 | MS.gene68663 | 0.812577 | 3.95E-51 | -1.69E-46 |
| MS.gene057705 | MS.gene68663 | 0.855168 | 7.56E-62 | -1.69E-46 |
| MS.gene057893 | MS.gene68663 | -0.804713 | 1.89E-49 | -1.69E-46 |
| MS.gene058008 | MS.gene68663 | 0.814707 | 1.34E-51 | -1.69E-46 |
| MS.gene058009 | MS.gene68663 | 0.801323 | 9.51E-49 | -1.69E-46 |
| MS.gene058216 | MS.gene68663 | 0.808819 | 2.57E-50 | -1.69E-46 |
| MS.gene058330 | MS.gene68663 | 0.83178 | 1.37E-55 | -1.69E-46 |
| MS.gene058492 | MS.gene68663 | 0.80448 | 2.12E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene68663 | 0.813388 | 2.63E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene68663 | 0.810229 | 1.28E-50 | -1.69E-46 |
| MS.gene058783 | MS.gene68663 | 0.813926 | 2.00E-51 | -1.69E-46 |
| MS.gene058852 | MS.gene68663 | 0.810073 | 1.38E-50 | -1.69E-46 |
| MS.gene05894 | MS.gene68663 | 0.851124 | 1.09E-60 | -1.69E-46 |
| MS.gene059015 | MS.gene68663 | 0.806979 | 6.32E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene68663 | 0.817261 | 3.61E-52 | -1.69E-46 |
| MS.gene059170 | MS.gene68663 | 0.801956 | 7.05E-49 | -1.69E-46 |
| MS.gene059227 | MS.gene68663 | 0.836207 | 1.07E-56 | -1.69E-46 |
| MS.gene05954 | MS.gene68663 | 0.814946 | 1.19E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene68663 | 0.845619 | 3.63E-59 | -1.69E-46 |
| MS.gene05985 | MS.gene68663 | -0.847816 | 9.10E-60 | -1.69E-46 |
| MS.gene059935 | MS.gene68663 | 0.831774 | 1.38E-55 | -1.69E-46 |
| MS.gene060365 | MS.gene68663 | 0.863534 | 2.32E-64 | -1.69E-46 |
| MS.gene060429 | MS.gene68663 | 0.849226 | 3.70E-60 | -1.69E-46 |
| MS.gene060512 | MS.gene68663 | 0.800779 | 1.23E-48 | -1.69E-46 |
| MS.gene060519 | MS.gene68663 | 0.814102 | 1.83E-51 | -1.69E-46 |
| MS.gene060521 | MS.gene68663 | 0.807061 | 6.07E-50 | -1.69E-46 |
| MS.gene060587 | MS.gene68663 | 0.827271 | 1.72E-54 | -1.69E-46 |
| MS.gene060603 | MS.gene68663 | 0.852652 | 4.01E-61 | -1.69E-46 |
| MS.gene060634 | MS.gene68663 | -0.802687 | 4.98E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene68663 | 0.813653 | 2.30E-51 | -1.69E-46 |
| MS.gene060937 | MS.gene68663 | 0.815095 | 1.10E-51 | -1.69E-46 |
| MS.gene061100 | MS.gene68663 | 0.868073 | 8.56E-66 | -1.69E-46 |
| MS.gene061160 | MS.gene68663 | 0.813838 | 2.09E-51 | -1.69E-46 |
| MS.gene061188 | MS.gene68663 | 0.813734 | 2.20E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene005994 | MS.gene68663 | PPI |
| MS.gene73243 | MS.gene68663 | PPI |
| MS.gene048096 | MS.gene68663 | PPI |
| MS.gene47051 | MS.gene68663 | PPI |
| MS.gene34008 | MS.gene68663 | PPI |
| MS.gene005996 | MS.gene68663 | PPI |
| MS.gene001098 | MS.gene68663 | PPI |
| MS.gene81545 | MS.gene68663 | PPI |
| MS.gene54761 | MS.gene68663 | PPI |
| MS.gene33103 | MS.gene68663 | PPI |
| MS.gene074184 | MS.gene68663 | PPI |
| MS.gene041771 | MS.gene68663 | PPI |
| MS.gene34006 | MS.gene68663 | PPI |
| MS.gene016105 | MS.gene68663 | PPI |
| MS.gene001097 | MS.gene68663 | PPI |
| MS.gene016914 | MS.gene68663 | PPI |
| MS.gene92572 | MS.gene68663 | PPI |
| MS.gene54763 | MS.gene68663 | PPI |
| MS.gene006000 | MS.gene68663 | PPI |
| MS.gene47052 | MS.gene68663 | PPI |
| MS.gene043773 | MS.gene68663 | PPI |
| MS.gene041769 | MS.gene68663 | PPI |
| MS.gene057052 | MS.gene68663 | PPI |
| MS.gene041766 | MS.gene68663 | PPI |
| MS.gene036405 | MS.gene68663 | PPI |
| MS.gene39764 | MS.gene68663 | PPI |
| MS.gene058469 | MS.gene68663 | PPI |
| MS.gene68663 | MS.gene92572 | PPI |
| MS.gene37588 | MS.gene68663 | PPI |
| MS.gene68663 | MS.gene037669 | PPI |
| MS.gene074183 | MS.gene68663 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68663.t1 | MTR_2g461200 | 99.134 | 231 | 2 | 0 | 1 | 231 | 1 | 231 | 1.23e-165 | 457 |
| MS.gene68663.t1 | MTR_2g461200 | 97.863 | 234 | 5 | 0 | 1 | 234 | 1 | 234 | 5.37e-140 | 393 |
| MS.gene68663.t1 | MTR_4g019610 | 94.850 | 233 | 12 | 0 | 1 | 233 | 1 | 233 | 2.91e-138 | 388 |
| MS.gene68663.t1 | MTR_4g019610 | 92.827 | 237 | 17 | 0 | 1 | 237 | 1 | 237 | 6.15e-137 | 386 |
| MS.gene68663.t1 | MTR_2g461200 | 97.778 | 180 | 4 | 0 | 1 | 180 | 1 | 180 | 3.62e-123 | 348 |
| MS.gene68663.t1 | MTR_5g088660 | 57.576 | 231 | 90 | 3 | 1 | 231 | 1 | 223 | 5.15e-80 | 240 |
| MS.gene68663.t1 | MTR_5g088685 | 75.000 | 48 | 12 | 0 | 133 | 180 | 2 | 49 | 9.72e-18 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68663.t1 | AT2G18110 | 80.088 | 226 | 44 | 1 | 7 | 231 | 6 | 231 | 7.69e-111 | 318 |
| MS.gene68663.t1 | AT1G30230 | 79.039 | 229 | 47 | 1 | 7 | 234 | 6 | 234 | 7.88e-109 | 315 |
| MS.gene68663.t1 | AT1G30230 | 79.204 | 226 | 46 | 1 | 7 | 231 | 6 | 231 | 7.97e-107 | 308 |
| MS.gene68663.t1 | AT5G12110 | 59.574 | 235 | 84 | 3 | 1 | 231 | 1 | 228 | 7.64e-79 | 237 |
| MS.gene68663.t1 | AT5G19510 | 57.576 | 231 | 91 | 2 | 1 | 231 | 1 | 224 | 1.38e-71 | 219 |
Find 59 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGACTATAGTGGTGTTTC+TGG | 0.119961 | 2.3:+39608842 | MS.gene68663:intron |
| TTCAGTTGGAAGGGTTGTTT+TGG | 0.210487 | 2.3:+39609246 | MS.gene68663:CDS |
| TCAGTTCCATCATATGAATA+TGG | 0.245099 | 2.3:+39608645 | MS.gene68663:CDS |
| TCTTGTTTCCGTTGACAATA+TGG | 0.314177 | 2.3:+39609937 | MS.gene68663:CDS |
| GCAGCCTTTGTGTCAGAAAC+TGG | 0.339173 | 2.3:-39608926 | None:intergenic |
| GAAGAAGGCAGCTGAGGAAC+GGG | 0.395604 | 2.3:+39609009 | MS.gene68663:CDS |
| GCAGCAGCCGTGAAGGCATC+TGG | 0.397283 | 2.3:+39609031 | MS.gene68663:CDS |
| TATAACCTTAAGGCTGAATC+TGG | 0.399774 | 2.3:+39608420 | MS.gene68663:CDS |
| GATGACGATGTTGATTTGTT+TGG | 0.412687 | 2.3:+39608968 | MS.gene68663:CDS |
| TCAAATTATGCTTACTATTG+TGG | 0.416944 | 2.3:+39609910 | MS.gene68663:CDS |
| TCAAACCAGATTCAGCCTTA+AGG | 0.419155 | 2.3:-39608425 | None:intergenic |
| TAGCCAAACTTGTTCCTGTT+GGG | 0.428630 | 2.3:+39609870 | MS.gene68663:intron |
| GGTGTTTCTGGTGAGGGATC+TGG | 0.436594 | 2.3:+39608854 | MS.gene68663:CDS |
| GTACCCAACAGGAACAAGTT+TGG | 0.443426 | 2.3:-39609873 | None:intergenic |
| ACTCTGGACATACTCATTGA+TGG | 0.454635 | 2.3:-39609984 | None:intergenic |
| TTCTTGATACCGTACCCAAC+AGG | 0.456829 | 2.3:-39609884 | None:intergenic |
| CAGTTCCATCATATGAATAT+GGG | 0.459928 | 2.3:+39608646 | MS.gene68663:CDS |
| TGGGAAATCCTCTGTATTGT+TGG | 0.471062 | 2.3:+39609163 | MS.gene68663:CDS |
| TTCCGTTGACAATATGGTTG+AGG | 0.479405 | 2.3:+39609943 | MS.gene68663:CDS |
| AGTTACACTCTATAACCTTA+AGG | 0.486396 | 2.3:+39608410 | MS.gene68663:CDS |
| TTCTTCAAGCTTCTTCATGT+CGG | 0.497830 | 2.3:-39609210 | None:intergenic |
| AGCAGTGAGATCTGTTCAGT+TGG | 0.499725 | 2.3:+39609232 | MS.gene68663:CDS |
| TTCGTATGACATGGAGATCA+TGG | 0.500082 | 2.3:+39610139 | MS.gene68663:CDS |
| ACATTCCCATATTCATATGA+TGG | 0.500557 | 2.3:-39608651 | None:intergenic |
| TGATGTCATCCTTTGAAGCT+TGG | 0.506163 | 2.3:-39608602 | None:intergenic |
| GTGAGATCTGTTCAGTTGGA+AGG | 0.509565 | 2.3:+39609236 | MS.gene68663:CDS |
| ATAGCCAAACTTGTTCCTGT+TGG | 0.511083 | 2.3:+39609869 | MS.gene68663:intron |
| AGAAGAAGGCAGCTGAGGAA+CGG | 0.514292 | 2.3:+39609008 | MS.gene68663:CDS |
| AAACTTGTTCCTGTTGGGTA+CGG | 0.517929 | 2.3:+39609875 | MS.gene68663:CDS |
| TATTCATATGATGGAACTGA+TGG | 0.520528 | 2.3:-39608642 | None:intergenic |
| AGAGACTGAGGAAGAGAAGA+AGG | 0.523236 | 2.3:+39608994 | MS.gene68663:CDS |
| CTTACACGCAGTTACATCAC+TGG | 0.529989 | 2.3:+39608468 | MS.gene68663:CDS |
| CTCTGGACATACTCATTGAT+GGG | 0.540335 | 2.3:-39609983 | None:intergenic |
| TGATGTAACTGCGTGTAAGA+AGG | 0.549903 | 2.3:-39608464 | None:intergenic |
| GTGTAGGTACCAAGCTTCAA+AGG | 0.552158 | 2.3:+39608593 | MS.gene68663:intron |
| ATGTCGGTCTCATCATCCCA+TGG | 0.564640 | 2.3:-39609194 | None:intergenic |
| CACAAAGGCTGCTGAAGCTG+AGG | 0.571919 | 2.3:+39608937 | MS.gene68663:CDS |
| GGAACGGGCAGCAGCCGTGA+AGG | 0.572363 | 2.3:+39609024 | MS.gene68663:CDS |
| TGAGATCTGTTCAGTTGGAA+GGG | 0.572783 | 2.3:+39609237 | MS.gene68663:CDS |
| TATAGTGGTGTTTCTGGTGA+GGG | 0.573048 | 2.3:+39608848 | MS.gene68663:intron |
| CTATAGTGGTGTTTCTGGTG+AGG | 0.574940 | 2.3:+39608847 | MS.gene68663:intron |
| ATCCTCAACCATATTGTCAA+CGG | 0.584339 | 2.3:-39609945 | None:intergenic |
| AGGCCACAATGTCACAACTC+TGG | 0.585892 | 2.3:-39610000 | None:intergenic |
| CACTGTCGAATCTTCTCTTG+TGG | 0.587184 | 2.3:+39608880 | MS.gene68663:CDS |
| ATGAATATGGGAATGTTGCT+AGG | 0.597917 | 2.3:+39608658 | MS.gene68663:CDS |
| GGAAGAGAAGAAGGCAGCTG+AGG | 0.604357 | 2.3:+39609003 | MS.gene68663:CDS |
| TATTGTTGGATGTGAAGCCA+TGG | 0.605647 | 2.3:+39609177 | MS.gene68663:CDS |
| TATGACATGGAGATCATGGT+AGG | 0.620700 | 2.3:+39610143 | MS.gene68663:CDS |
| TGTGTCAGAAACTGGAGGAG+TGG | 0.624152 | 2.3:-39608918 | None:intergenic |
| TTACACGCAGTTACATCACT+GGG | 0.625744 | 2.3:+39608469 | MS.gene68663:CDS |
| TCCTCCAGTTTCTGACACAA+AGG | 0.631021 | 2.3:+39608922 | MS.gene68663:CDS |
| TGTCCAGAGTTGTGACATTG+TGG | 0.639251 | 2.3:+39609997 | MS.gene68663:CDS |
| GACATGGAGATCATGGTAGG+AGG | 0.643475 | 2.3:+39610146 | MS.gene68663:CDS |
| AATCTGCAATTCGTATGACA+TGG | 0.645534 | 2.3:+39610130 | MS.gene68663:CDS |
| ATTGTTGGATGTGAAGCCAT+GGG | 0.652625 | 2.3:+39609178 | MS.gene68663:CDS |
| GCCTTTGTGTCAGAAACTGG+AGG | 0.658117 | 2.3:-39608923 | None:intergenic |
| TTTGTTTGGTGAAGAGACTG+AGG | 0.673347 | 2.3:+39608982 | MS.gene68663:CDS |
| CTTCACATCCAACAATACAG+AGG | 0.716868 | 2.3:-39609171 | None:intergenic |
| AATATGGGAATGTTGCTAGG+TGG | 0.778608 | 2.3:+39608661 | MS.gene68663:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCGACAAAAAAATAAAAA+TGG | - | chr2.3:39609299-39609318 | None:intergenic | 15.0% |
| !!! | TTATTGTATACTATTTTTCA+AGG | + | chr2.3:39609578-39609597 | MS.gene68663:intron | 15.0% |
| !! | AAAATAAGATATTTCGCTAT+TGG | - | chr2.3:39609717-39609736 | None:intergenic | 20.0% |
| !! | ATAAACTAATATCAGAATCA+TGG | - | chr2.3:39609842-39609861 | None:intergenic | 20.0% |
| !! | ATAGTATACAATAAACTGAA+TGG | - | chr2.3:39609572-39609591 | None:intergenic | 20.0% |
| !! | TTCATATCCTCATAATTATT+TGG | + | chr2.3:39609085-39609104 | MS.gene68663:intron | 20.0% |
| !!! | TTACTTACAGATTTTATTGA+AGG | - | chr2.3:39610023-39610042 | None:intergenic | 20.0% |
| !!! | TTGTTGTATACTATTTTTCA+AGG | + | chr2.3:39609389-39609408 | MS.gene68663:intron | 20.0% |
| ! | ATAATTGACAAAGATGGTAA+AGG | - | chr2.3:39608727-39608746 | None:intergenic | 25.0% |
| ! | TAATGCCATATCATATTCTT+TGG | + | chr2.3:39610102-39610121 | MS.gene68663:intron | 25.0% |
| ! | TAATTGACAAAGATGGTAAA+GGG | - | chr2.3:39608726-39608745 | None:intergenic | 25.0% |
| ! | TCAAATTATGCTTACTATTG+TGG | + | chr2.3:39609910-39609929 | MS.gene68663:CDS | 25.0% |
| ! | TCGAATATAATTGACAAAGA+TGG | - | chr2.3:39608733-39608752 | None:intergenic | 25.0% |
| !! | ATAATTATTTGGAATCTGGT+TGG | + | chr2.3:39609096-39609115 | MS.gene68663:intron | 25.0% |
| !!! | ATAAGTTTGTTTTCCACTAT+TGG | + | chr2.3:39609641-39609660 | MS.gene68663:intron | 25.0% |
| !!! | ATATGTTTGTTTTCCACTAT+TGG | + | chr2.3:39609453-39609472 | MS.gene68663:intron | 25.0% |
| !!! | TAAGTTTGTTTTCCACTATT+GGG | + | chr2.3:39609642-39609661 | MS.gene68663:intron | 25.0% |
| !!! | TATGTTTGTTTTCCACTATT+GGG | + | chr2.3:39609454-39609473 | MS.gene68663:intron | 25.0% |
| !!! | TGTTTTTATTTGATCTGTGT+AGG | + | chr2.3:39608577-39608596 | MS.gene68663:intron | 25.0% |
| !!! | TTCAGATTTTATTTGTCTGT+TGG | + | chr2.3:39610045-39610064 | MS.gene68663:intron | 25.0% |
| ACATTCCCATATTCATATGA+TGG | - | chr2.3:39608654-39608673 | None:intergenic | 30.0% | |
| AGTTACACTCTATAACCTTA+AGG | + | chr2.3:39608410-39608429 | MS.gene68663:CDS | 30.0% | |
| ATGGTGCACAAATAAGATAT+TGG | + | chr2.3:39609608-39609627 | MS.gene68663:intron | 30.0% | |
| CAGTTCCATCATATGAATAT+GGG | + | chr2.3:39608646-39608665 | MS.gene68663:CDS | 30.0% | |
| CCAGATTCCAAATAATTATG+AGG | - | chr2.3:39609095-39609114 | None:intergenic | 30.0% | |
| TCAGTTCCATCATATGAATA+TGG | + | chr2.3:39608645-39608664 | MS.gene68663:CDS | 30.0% | |
| TCATATCTCACTTGATCTAA+TGG | + | chr2.3:39609490-39609509 | MS.gene68663:intron | 30.0% | |
| ! | AACAAGTTTGCTTTCACTAT+TGG | - | chr2.3:39609529-39609548 | None:intergenic | 30.0% |
| ! | CCTCATAATTATTTGGAATC+TGG | + | chr2.3:39609092-39609111 | MS.gene68663:intron | 30.0% |
| ! | TATTCATATGATGGAACTGA+TGG | - | chr2.3:39608645-39608664 | None:intergenic | 30.0% |
| ! | TGGACATCAATTTAGAGTAT+TGG | + | chr2.3:39609116-39609135 | MS.gene68663:intron | 30.0% |
| !! | ATTGAGTTTTGTGACTATAG+TGG | + | chr2.3:39608833-39608852 | MS.gene68663:intron | 30.0% |
| !!! | ATTTTTCAAGGCTATGTCTA+CGG | + | chr2.3:39609401-39609420 | MS.gene68663:intron | 30.0% |
| !!! | TATTTTTCAAGGCATGTCTA+TGG | + | chr2.3:39609589-39609608 | MS.gene68663:intron | 30.0% |
| AATCTGCAATTCGTATGACA+TGG | + | chr2.3:39610130-39610149 | MS.gene68663:CDS | 35.0% | |
| ACGGTGCACAAATAAGATAT+TGG | + | chr2.3:39609420-39609439 | MS.gene68663:intron | 35.0% | |
| ATCCTCAACCATATTGTCAA+CGG | - | chr2.3:39609948-39609967 | None:intergenic | 35.0% | |
| ATGAATATGGGAATGTTGCT+AGG | + | chr2.3:39608658-39608677 | MS.gene68663:CDS | 35.0% | |
| CTGCACCAAAGAATATGATA+TGG | - | chr2.3:39610110-39610129 | None:intergenic | 35.0% | |
| GTGGAAAACAAACATATGCA+TGG | - | chr2.3:39609450-39609469 | None:intergenic | 35.0% | |
| TAGATCAAGCGAGATATGAA+GGG | - | chr2.3:39609677-39609696 | None:intergenic | 35.0% | |
| TATAACCTTAAGGCTGAATC+TGG | + | chr2.3:39608420-39608439 | MS.gene68663:CDS | 35.0% | |
| TATCTCACTTGATCTAATGG+TGG | + | chr2.3:39609493-39609512 | MS.gene68663:intron | 35.0% | |
| TCATATCTCGCTTGATCTAA+TGG | + | chr2.3:39609678-39609697 | MS.gene68663:intron | 35.0% | |
| TTAGATCAAGCGAGATATGA+AGG | - | chr2.3:39609678-39609697 | None:intergenic | 35.0% | |
| TTCTTCAAGCTTCTTCATGT+CGG | - | chr2.3:39609213-39609232 | None:intergenic | 35.0% | |
| ! | AGTGAAAGCAAACTTGTTCA+CGG | + | chr2.3:39609531-39609550 | MS.gene68663:intron | 35.0% |
| ! | TCTTGTTTCCGTTGACAATA+TGG | + | chr2.3:39609937-39609956 | MS.gene68663:CDS | 35.0% |
| ! | TTTCTGCTGATTGCATTATC+AGG | + | chr2.3:39609140-39609159 | MS.gene68663:intron | 35.0% |
| !! | GATGACGATGTTGATTTGTT+TGG | + | chr2.3:39608968-39608987 | MS.gene68663:CDS | 35.0% |
| AATATGGGAATGTTGCTAGG+TGG | + | chr2.3:39608661-39608680 | MS.gene68663:CDS | 40.0% | |
| ACTCTGGACATACTCATTGA+TGG | - | chr2.3:39609987-39610006 | None:intergenic | 40.0% | |
| ATTGTTGGATGTGAAGCCAT+GGG | + | chr2.3:39609178-39609197 | MS.gene68663:CDS | 40.0% | |
| CTCTGGACATACTCATTGAT+GGG | - | chr2.3:39609986-39610005 | None:intergenic | 40.0% | |
| CTTCACATCCAACAATACAG+AGG | - | chr2.3:39609174-39609193 | None:intergenic | 40.0% | |
| TATCTCGCTTGATCTAATGG+TGG | + | chr2.3:39609681-39609700 | MS.gene68663:intron | 40.0% | |
| TATGACATGGAGATCATGGT+AGG | + | chr2.3:39610143-39610162 | MS.gene68663:CDS | 40.0% | |
| TATTGTTGGATGTGAAGCCA+TGG | + | chr2.3:39609177-39609196 | MS.gene68663:CDS | 40.0% | |
| TCAAACCAGATTCAGCCTTA+AGG | - | chr2.3:39608428-39608447 | None:intergenic | 40.0% | |
| TGAGATCTGTTCAGTTGGAA+GGG | + | chr2.3:39609237-39609256 | MS.gene68663:CDS | 40.0% | |
| TGATGTAACTGCGTGTAAGA+AGG | - | chr2.3:39608467-39608486 | None:intergenic | 40.0% | |
| TGGGAAATCCTCTGTATTGT+TGG | + | chr2.3:39609163-39609182 | MS.gene68663:CDS | 40.0% | |
| TGTGACTATAGTGGTGTTTC+TGG | + | chr2.3:39608842-39608861 | MS.gene68663:intron | 40.0% | |
| TTACACGCAGTTACATCACT+GGG | + | chr2.3:39608469-39608488 | MS.gene68663:CDS | 40.0% | |
| TTCCGTTGACAATATGGTTG+AGG | + | chr2.3:39609943-39609962 | MS.gene68663:CDS | 40.0% | |
| TTCGTATGACATGGAGATCA+TGG | + | chr2.3:39610139-39610158 | MS.gene68663:CDS | 40.0% | |
| ! | AAACTTGTTCCTGTTGGGTA+CGG | + | chr2.3:39609875-39609894 | MS.gene68663:CDS | 40.0% |
| ! | ATAGCCAAACTTGTTCCTGT+TGG | + | chr2.3:39609869-39609888 | MS.gene68663:intron | 40.0% |
| ! | TAGCCAAACTTGTTCCTGTT+GGG | + | chr2.3:39609870-39609889 | MS.gene68663:intron | 40.0% |
| ! | TGCTGATTGCATTATCAGGT+GGG | + | chr2.3:39609144-39609163 | MS.gene68663:intron | 40.0% |
| ! | TTCAGTTGGAAGGGTTGTTT+TGG | + | chr2.3:39609246-39609265 | MS.gene68663:CDS | 40.0% |
| ! | TTTGTTTGGTGAAGAGACTG+AGG | + | chr2.3:39608982-39609001 | MS.gene68663:CDS | 40.0% |
| !! | TATAGTGGTGTTTCTGGTGA+GGG | + | chr2.3:39608848-39608867 | MS.gene68663:intron | 40.0% |
| !! | TCAGTTGGAAGGGTTGTTTT+GGG | + | chr2.3:39609247-39609266 | MS.gene68663:CDS | 40.0% |
| !! | TGATGTCATCCTTTGAAGCT+TGG | - | chr2.3:39608605-39608624 | None:intergenic | 40.0% |
| AGAGACTGAGGAAGAGAAGA+AGG | + | chr2.3:39608994-39609013 | MS.gene68663:CDS | 45.0% | |
| AGCAGTGAGATCTGTTCAGT+TGG | + | chr2.3:39609232-39609251 | MS.gene68663:CDS | 45.0% | |
| CACTGTCGAATCTTCTCTTG+TGG | + | chr2.3:39608880-39608899 | MS.gene68663:CDS | 45.0% | |
| CTTACACGCAGTTACATCAC+TGG | + | chr2.3:39608468-39608487 | MS.gene68663:CDS | 45.0% | |
| GCGACTTAATGACCCAATAG+TGG | - | chr2.3:39609469-39609488 | None:intergenic | 45.0% | |
| GGGACTTATTGACCCAATAG+TGG | - | chr2.3:39609657-39609676 | None:intergenic | 45.0% | |
| GTACCCAACAGGAACAAGTT+TGG | - | chr2.3:39609876-39609895 | None:intergenic | 45.0% | |
| GTGAGATCTGTTCAGTTGGA+AGG | + | chr2.3:39609236-39609255 | MS.gene68663:CDS | 45.0% | |
| GTGTAGGTACCAAGCTTCAA+AGG | + | chr2.3:39608593-39608612 | MS.gene68663:intron | 45.0% | |
| TCCTCCAGTTTCTGACACAA+AGG | + | chr2.3:39608922-39608941 | MS.gene68663:CDS | 45.0% | |
| TGTCCAGAGTTGTGACATTG+TGG | + | chr2.3:39609997-39610016 | MS.gene68663:CDS | 45.0% | |
| TTCTTGATACCGTACCCAAC+AGG | - | chr2.3:39609887-39609906 | None:intergenic | 45.0% | |
| ! | CTGCTGATTGCATTATCAGG+TGG | + | chr2.3:39609143-39609162 | MS.gene68663:intron | 45.0% |
| !! | CAGTTGGAAGGGTTGTTTTG+GGG | + | chr2.3:39609248-39609267 | MS.gene68663:CDS | 45.0% |
| !! | CTATAGTGGTGTTTCTGGTG+AGG | + | chr2.3:39608847-39608866 | MS.gene68663:intron | 45.0% |
| ACGCAGTTACATCACTGGGT+AGG | + | chr2.3:39608473-39608492 | MS.gene68663:intron | 50.0% | |
| AGAAGAAGGCAGCTGAGGAA+CGG | + | chr2.3:39609008-39609027 | MS.gene68663:CDS | 50.0% | |
| AGGCCACAATGTCACAACTC+TGG | - | chr2.3:39610003-39610022 | None:intergenic | 50.0% | |
| ATGTCGGTCTCATCATCCCA+TGG | - | chr2.3:39609197-39609216 | None:intergenic | 50.0% | |
| GACATGGAGATCATGGTAGG+AGG | + | chr2.3:39610146-39610165 | MS.gene68663:CDS | 50.0% | |
| GCAGCCTTTGTGTCAGAAAC+TGG | - | chr2.3:39608929-39608948 | None:intergenic | 50.0% | |
| GCCTTTGTGTCAGAAACTGG+AGG | - | chr2.3:39608926-39608945 | None:intergenic | 50.0% | |
| TGTGTCAGAAACTGGAGGAG+TGG | - | chr2.3:39608921-39608940 | None:intergenic | 50.0% | |
| ! | CTTTTCGCCAGATGCCTTCA+CGG | - | chr2.3:39609041-39609060 | None:intergenic | 50.0% |
| CACAAAGGCTGCTGAAGCTG+AGG | + | chr2.3:39608937-39608956 | MS.gene68663:CDS | 55.0% | |
| GAAGAAGGCAGCTGAGGAAC+GGG | + | chr2.3:39609009-39609028 | MS.gene68663:CDS | 55.0% | |
| GGAAGAGAAGAAGGCAGCTG+AGG | + | chr2.3:39609003-39609022 | MS.gene68663:CDS | 55.0% | |
| !! | GGTGTTTCTGGTGAGGGATC+TGG | + | chr2.3:39608854-39608873 | MS.gene68663:CDS | 55.0% |
| !! | GCAGCAGCCGTGAAGGCATC+TGG | + | chr2.3:39609031-39609050 | MS.gene68663:CDS | 65.0% |
| GGAACGGGCAGCAGCCGTGA+AGG | + | chr2.3:39609024-39609043 | MS.gene68663:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 39608405 | 39610187 | 39608405 | ID=MS.gene68663 |
| chr2.3 | mRNA | 39608405 | 39610187 | 39608405 | ID=MS.gene68663.t1;Parent=MS.gene68663 |
| chr2.3 | exon | 39608405 | 39608490 | 39608405 | ID=MS.gene68663.t1.exon1;Parent=MS.gene68663.t1 |
| chr2.3 | CDS | 39608405 | 39608490 | 39608405 | ID=cds.MS.gene68663.t1;Parent=MS.gene68663.t1 |
| chr2.3 | exon | 39608599 | 39608715 | 39608599 | ID=MS.gene68663.t1.exon2;Parent=MS.gene68663.t1 |
| chr2.3 | CDS | 39608599 | 39608715 | 39608599 | ID=cds.MS.gene68663.t1;Parent=MS.gene68663.t1 |
| chr2.3 | exon | 39608853 | 39609067 | 39608853 | ID=MS.gene68663.t1.exon3;Parent=MS.gene68663.t1 |
| chr2.3 | CDS | 39608853 | 39609067 | 39608853 | ID=cds.MS.gene68663.t1;Parent=MS.gene68663.t1 |
| chr2.3 | exon | 39609162 | 39609275 | 39609162 | ID=MS.gene68663.t1.exon4;Parent=MS.gene68663.t1 |
| chr2.3 | CDS | 39609162 | 39609275 | 39609162 | ID=cds.MS.gene68663.t1;Parent=MS.gene68663.t1 |
| chr2.3 | exon | 39609873 | 39610038 | 39609873 | ID=MS.gene68663.t1.exon5;Parent=MS.gene68663.t1 |
| chr2.3 | CDS | 39609873 | 39610038 | 39609873 | ID=cds.MS.gene68663.t1;Parent=MS.gene68663.t1 |
| chr2.3 | exon | 39610130 | 39610187 | 39610130 | ID=MS.gene68663.t1.exon6;Parent=MS.gene68663.t1 |
| chr2.3 | CDS | 39610130 | 39610187 | 39610130 | ID=cds.MS.gene68663.t1;Parent=MS.gene68663.t1 |
| Gene Sequence |
| Protein sequence |
