AlfalfaGEDB | MS.gene93204

M. sativa cultivar XinJiangDaYe / MS.gene93204

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93204.t1	KEH21095.1	97.4	152	4	0	1	152	83	234	4.10E-59	237.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93204.t1	Q84JT7	54.8	93	39	2	63	152	362	454	2.4e-23	110.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93204.t1	A0A072U5F8	97.4	152	4	0	1	152	83	234	3.0e-59	237.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene93204	0.859385	4.29E-63	-1.69E-46
MS.gene049159	MS.gene93204	0.816281	6.00E-52	-1.69E-46
MS.gene049254	MS.gene93204	0.802229	6.19E-49	-1.69E-46
MS.gene049368	MS.gene93204	0.825566	4.37E-54	-1.69E-46
MS.gene049786	MS.gene93204	0.802001	6.90E-49	-1.69E-46
MS.gene049850	MS.gene93204	0.804115	2.52E-49	-1.69E-46
MS.gene049983	MS.gene93204	0.824971	6.04E-54	-1.69E-46
MS.gene050551	MS.gene93204	0.808111	3.64E-50	-1.69E-46
MS.gene050654	MS.gene93204	0.82981	4.18E-55	-1.69E-46
MS.gene050759	MS.gene93204	0.821059	4.94E-53	-1.69E-46
MS.gene050941	MS.gene93204	0.801646	8.16E-49	-1.69E-46
MS.gene050962	MS.gene93204	0.810187	1.31E-50	-1.69E-46
MS.gene051198	MS.gene93204	0.804187	2.44E-49	-1.69E-46
MS.gene051247	MS.gene93204	0.815651	8.29E-52	-1.69E-46
MS.gene051363	MS.gene93204	0.818099	2.34E-52	-1.69E-46
MS.gene051365	MS.gene93204	0.802147	6.44E-49	-1.69E-46
MS.gene051490	MS.gene93204	0.803231	3.85E-49	-1.69E-46
MS.gene051700	MS.gene93204	0.802276	6.06E-49	-1.69E-46
MS.gene051744	MS.gene93204	0.801343	9.42E-49	-1.69E-46
MS.gene051800	MS.gene93204	0.814906	1.21E-51	-1.69E-46
MS.gene05188	MS.gene93204	0.81515	1.07E-51	-1.69E-46
MS.gene051975	MS.gene93204	0.811076	8.39E-51	-1.69E-46
MS.gene052012	MS.gene93204	0.812597	3.91E-51	-1.69E-46
MS.gene052463	MS.gene93204	0.835197	1.93E-56	-1.69E-46
MS.gene052516	MS.gene93204	0.820129	8.07E-53	-1.69E-46
MS.gene052609	MS.gene93204	0.806487	8.03E-50	-1.69E-46
MS.gene052773	MS.gene93204	0.813879	2.05E-51	-1.69E-46
MS.gene053008	MS.gene93204	0.812116	4.98E-51	-1.69E-46
MS.gene053079	MS.gene93204	0.824403	8.23E-54	-1.69E-46
MS.gene053226	MS.gene93204	0.828302	9.70E-55	-1.69E-46
MS.gene054139	MS.gene93204	0.832538	8.93E-56	-1.69E-46
MS.gene054479	MS.gene93204	0.822857	1.89E-53	-1.69E-46
MS.gene054576	MS.gene93204	0.800532	1.38E-48	-1.69E-46
MS.gene05458	MS.gene93204	0.807019	6.20E-50	-1.69E-46
MS.gene054651	MS.gene93204	0.815687	8.14E-52	-1.69E-46
MS.gene054775	MS.gene93204	0.803616	3.20E-49	-1.69E-46
MS.gene054875	MS.gene93204	0.80682	6.83E-50	-1.69E-46
MS.gene05517	MS.gene93204	0.827827	1.26E-54	-1.69E-46
MS.gene055313	MS.gene93204	0.813448	2.55E-51	-1.69E-46
MS.gene055466	MS.gene93204	0.802153	6.42E-49	-1.69E-46
MS.gene055469	MS.gene93204	0.80469	1.91E-49	-1.69E-46
MS.gene055513	MS.gene93204	0.855374	6.59E-62	-1.69E-46
MS.gene055864	MS.gene93204	0.840529	8.23E-58	-1.69E-46
MS.gene055926	MS.gene93204	0.824165	9.36E-54	-1.69E-46
MS.gene056045	MS.gene93204	0.826249	3.01E-54	-1.69E-46
MS.gene05604	MS.gene93204	0.804493	2.10E-49	-1.69E-46
MS.gene056181	MS.gene93204	0.820364	7.13E-53	-1.69E-46
MS.gene056308	MS.gene93204	0.827325	1.66E-54	-1.69E-46
MS.gene056390	MS.gene93204	0.838111	3.49E-57	-1.69E-46
MS.gene056509	MS.gene93204	0.816625	5.02E-52	-1.69E-46
MS.gene05686	MS.gene93204	0.800809	1.21E-48	-1.69E-46
MS.gene057034	MS.gene93204	0.831785	1.37E-55	-1.69E-46
MS.gene057208	MS.gene93204	0.815855	7.46E-52	-1.69E-46
MS.gene057360	MS.gene93204	0.808295	3.32E-50	-1.69E-46
MS.gene057693	MS.gene93204	0.815138	1.08E-51	-1.69E-46
MS.gene057915	MS.gene93204	0.82786	1.24E-54	-1.69E-46
MS.gene058010	MS.gene93204	0.800471	1.42E-48	-1.69E-46
MS.gene058081	MS.gene93204	0.815198	1.05E-51	-1.69E-46
MS.gene058292	MS.gene93204	0.809135	2.20E-50	-1.69E-46
MS.gene05897	MS.gene93204	0.825028	5.86E-54	-1.69E-46
MS.gene059283	MS.gene93204	0.807806	4.22E-50	-1.69E-46
MS.gene059439	MS.gene93204	0.820744	5.84E-53	-1.69E-46
MS.gene059570	MS.gene93204	0.813811	2.12E-51	-1.69E-46
MS.gene059725	MS.gene93204	0.824029	1.01E-53	-1.69E-46
MS.gene059842	MS.gene93204	0.811836	5.74E-51	-1.69E-46
MS.gene060296	MS.gene93204	0.829193	5.90E-55	-1.69E-46
MS.gene060365	MS.gene93204	0.824959	6.09E-54	-1.69E-46
MS.gene060435	MS.gene93204	0.815006	1.15E-51	-1.69E-46
MS.gene06052	MS.gene93204	0.824455	8.00E-54	-1.69E-46
MS.gene060637	MS.gene93204	0.81609	6.61E-52	-1.69E-46
MS.gene060802	MS.gene93204	0.800095	1.69E-48	-1.69E-46
MS.gene060803	MS.gene93204	0.821902	3.15E-53	-1.69E-46
MS.gene060933	MS.gene93204	0.822973	1.78E-53	-1.69E-46
MS.gene060935	MS.gene93204	0.808917	2.45E-50	-1.69E-46
MS.gene060937	MS.gene93204	0.817281	3.58E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93204.t1	MTR_8g098645	97.368	152	4	0	1	152	83	234	5.57e-108	312
MS.gene93204.t1	MTR_1g073380	73.684	152	39	1	1	152	336	486	1.00e-72	229
MS.gene93204.t1	MTR_8g098605	73.026	152	16	2	1	152	358	484	8.21e-69	219
MS.gene93204.t1	MTR_1g073530	69.737	152	43	1	1	152	328	476	7.48e-65	208
MS.gene93204.t1	MTR_1g073590	69.079	152	44	1	1	152	325	473	1.95e-64	207
MS.gene93204.t1	MTR_8g098635	89.655	87	9	0	66	152	99	185	1.99e-52	169
MS.gene93204.t1	MTR_8g098635	89.655	87	9	0	66	152	182	268	2.17e-51	169
MS.gene93204.t1	MTR_6g071350	75.258	97	24	0	62	158	273	369	5.14e-46	155
MS.gene93204.t1	MTR_8g028905	58.065	93	38	1	61	152	408	500	2.43e-31	118
MS.gene93204.t1	MTR_0259s0060	51.240	121	42	4	59	171	14	125	7.02e-31	110
MS.gene93204.t1	MTR_7g078120	56.044	91	40	0	61	151	387	477	7.70e-30	114
MS.gene93204.t1	MTR_7g078120	56.044	91	40	0	61	151	387	477	8.99e-30	114
MS.gene93204.t1	MTR_5g048010	54.237	118	38	2	42	158	182	284	6.37e-29	108
MS.gene93204.t1	MTR_7g066890	40.404	99	51	2	60	152	193	289	5.48e-15	71.6
MS.gene93204.t1	MTR_7g066890	40.404	99	51	2	60	152	295	391	8.41e-15	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93204.t1	AT2G46040	54.839	93	39	2	63	152	362	454	4.05e-28	109
MS.gene93204.t1	AT2G46040	54.839	93	39	2	63	152	362	454	4.05e-28	109
MS.gene93204.t1	AT2G46040	54.839	93	39	2	63	152	376	468	4.72e-28	109
MS.gene93204.t1	AT4G11400	43.200	125	64	2	30	152	348	467	1.14e-25	102
MS.gene93204.t1	AT4G11400	43.200	125	64	2	30	152	348	467	1.14e-25	102
MS.gene93204.t1	AT5G04110	35.294	102	55	3	61	152	361	461	3.79e-12	63.9
MS.gene93204.t1	AT5G04110	35.294	102	55	3	61	152	362	462	3.87e-12	63.9
MS.gene93204.t1	AT5G04110	35.294	102	55	3	61	152	361	461	4.18e-12	63.5
MS.gene93204.t1	AT5G04110	35.294	102	55	3	61	152	361	461	4.18e-12	63.5

Find 29 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATTGGGGATTTGATAAAA+TGG	0.249466	4.2:-6251126	MS.gene93204:CDS
TGATGAAGAAAAGAGATTTA+AGG	0.278264	4.2:-6251076	MS.gene93204:CDS
ACAAGATCTGTTTCGGTAGT+AGG	0.317915	4.2:+6251266	None:intergenic
TTTCTCTTCTCAGTTGCATC+TGG	0.327601	4.2:+6251479	None:intergenic
CTTCTCAGTTGCATCTGGTT+TGG	0.334667	4.2:+6251484	None:intergenic
GAGAAAATCTGAGCCATCTA+TGG	0.335639	4.2:-6251426	MS.gene93204:CDS
TTTCTGAGAGTGACTCTAAA+TGG	0.355739	4.2:-6251328	MS.gene93204:CDS
TTCAGTGACGACGTCTGGTT+TGG	0.357587	4.2:+6251523	None:intergenic
AATGGTTAGGCACACAAGTA+TGG	0.373689	4.2:-6251310	MS.gene93204:CDS
CGAAAATCATCATCCATAGA+TGG	0.407291	4.2:+6251413	None:intergenic
TCTCCCAACAAGATCTGTTT+CGG	0.428135	4.2:+6251259	None:intergenic
GATTTCATATTGCTGAGAAC+AGG	0.435888	4.2:-6251184	MS.gene93204:CDS
CCGGAACTTCCGCTTGGAAA+AGG	0.440137	4.2:+6251366	None:intergenic
GAAACTACACCAGTCCATTC+CGG	0.442162	4.2:+6251347	None:intergenic
CTACCGAAACAGATCTTGTT+GGG	0.462379	4.2:-6251262	MS.gene93204:CDS
CGTGAAAAGCTTGTTTCTGT+AGG	0.477267	4.2:-6251392	MS.gene93204:CDS
GAGAGTGACTCTAAATGGTT+AGG	0.492462	4.2:-6251323	MS.gene93204:CDS
AAGTGTAGCTGCAACGTTCA+AGG	0.494639	4.2:-6251224	MS.gene93204:CDS
GGAGTCAGAAATATGTCCTC+TGG	0.498373	4.2:-6251558	MS.gene93204:CDS
ACTACCGAAACAGATCTTGT+TGG	0.499677	4.2:-6251263	MS.gene93204:CDS
GTGAGGAAGTTTCACTTCGA+TGG	0.500579	4.2:-6251104	MS.gene93204:CDS
TCCATTCCGGAACTTCCGCT+TGG	0.513689	4.2:+6251360	None:intergenic
GCGGAAGTTCCGGAATGGAC+TGG	0.543878	4.2:-6251356	MS.gene93204:CDS
TCCAAGCGGAAGTTCCGGAA+TGG	0.566498	4.2:-6251361	MS.gene93204:CDS
GAAACAGATCTTGTTGGGAG+AGG	0.634208	4.2:-6251257	MS.gene93204:CDS
CTTGTTGGGAGAGGAAGACG+AGG	0.636192	4.2:-6251248	MS.gene93204:CDS
GGGGATTTGATAAAATGGTG+AGG	0.648403	4.2:-6251121	MS.gene93204:CDS
AGAGATTTAAGGATGCAATG+AGG	0.659010	4.2:-6251065	None:intergenic
AAATCCCCAATGATAAAACA+CGG	0.674548	4.2:+6251136	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGATGAAGAAAAGAGATTTA+AGG	-	chr4.2:6251551-6251570	MS.gene93204:CDS	25.0%
!!	TTCTCTTTTCAGTAATATCT+GGG	+	chr4.2:6251186-6251205	None:intergenic	25.0%
!!	TTTCTCTTTTCAGTAATATC+TGG	+	chr4.2:6251187-6251206	None:intergenic	25.0%
	AAATCCCCAATGATAAAACA+CGG	+	chr4.2:6251494-6251513	None:intergenic	30.0%
	TCATTGGGGATTTGATAAAA+TGG	-	chr4.2:6251501-6251520	MS.gene93204:CDS	30.0%
!!!	GGTTTTGAATCATCTTTAAC+AGG	+	chr4.2:6251343-6251362	None:intergenic	30.0%
	CGAAAATCATCATCCATAGA+TGG	+	chr4.2:6251217-6251236	None:intergenic	35.0%
	GATTTCATATTGCTGAGAAC+AGG	-	chr4.2:6251443-6251462	MS.gene93204:CDS	35.0%
!	CATTCCGTGTTTTATCATTG+GGG	-	chr4.2:6251487-6251506	MS.gene93204:CDS	35.0%
!	CTTTTCAGTAATATCTGGGT+TGG	+	chr4.2:6251182-6251201	None:intergenic	35.0%
!	GCATTCCGTGTTTTATCATT+GGG	-	chr4.2:6251486-6251505	MS.gene93204:CDS	35.0%
!	TGCATTCCGTGTTTTATCAT+TGG	-	chr4.2:6251485-6251504	MS.gene93204:CDS	35.0%
!	TTTCTGAGAGTGACTCTAAA+TGG	-	chr4.2:6251299-6251318	MS.gene93204:CDS	35.0%
	AATGGTTAGGCACACAAGTA+TGG	-	chr4.2:6251317-6251336	MS.gene93204:CDS	40.0%
	ACAAGATCTGTTTCGGTAGT+AGG	+	chr4.2:6251364-6251383	None:intergenic	40.0%
	ACTACCGAAACAGATCTTGT+TGG	-	chr4.2:6251364-6251383	MS.gene93204:CDS	40.0%
	CTACCGAAACAGATCTTGTT+GGG	-	chr4.2:6251365-6251384	MS.gene93204:CDS	40.0%
	GAGAAAATCTGAGCCATCTA+TGG	-	chr4.2:6251201-6251220	MS.gene93204:CDS	40.0%
	GAGAGTGACTCTAAATGGTT+AGG	-	chr4.2:6251304-6251323	MS.gene93204:CDS	40.0%
	GGGGATTTGATAAAATGGTG+AGG	-	chr4.2:6251506-6251525	MS.gene93204:CDS	40.0%
	TCTCCCAACAAGATCTGTTT+CGG	+	chr4.2:6251371-6251390	None:intergenic	40.0%
	TTTCTCTTCTCAGTTGCATC+TGG	+	chr4.2:6251151-6251170	None:intergenic	40.0%
!!	CGTGAAAAGCTTGTTTCTGT+AGG	-	chr4.2:6251235-6251254	MS.gene93204:CDS	40.0%
	AAGTGTAGCTGCAACGTTCA+AGG	-	chr4.2:6251403-6251422	MS.gene93204:CDS	45.0%
	CTTCTCAGTTGCATCTGGTT+TGG	+	chr4.2:6251146-6251165	None:intergenic	45.0%
	GAAACAGATCTTGTTGGGAG+AGG	-	chr4.2:6251370-6251389	MS.gene93204:CDS	45.0%
	GAAACTACACCAGTCCATTC+CGG	+	chr4.2:6251283-6251302	None:intergenic	45.0%
	GGAGTCAGAAATATGTCCTC+TGG	-	chr4.2:6251069-6251088	MS.gene93204:CDS	45.0%
	GTGAGGAAGTTTCACTTCGA+TGG	-	chr4.2:6251523-6251542	MS.gene93204:CDS	45.0%
!	TGTAGGTCACCTTTTCCAAG+CGG	-	chr4.2:6251252-6251271	MS.gene93204:CDS	45.0%
!	TTCTTTTCAGTGACGACGTC+TGG	+	chr4.2:6251112-6251131	None:intergenic	45.0%
!!!	TTGGCTGTTGTTTTCTCCAG+AGG	+	chr4.2:6251088-6251107	None:intergenic	45.0%
	TTCAGTGACGACGTCTGGTT+TGG	+	chr4.2:6251107-6251126	None:intergenic	50.0%
!	CCTTTTCCAAGCGGAAGTTC+CGG	-	chr4.2:6251261-6251280	MS.gene93204:CDS	50.0%
	CCGGAACTTCCGCTTGGAAA+AGG	+	chr4.2:6251264-6251283	None:intergenic	55.0%
	CTTGTTGGGAGAGGAAGACG+AGG	-	chr4.2:6251379-6251398	MS.gene93204:CDS	55.0%
	TCCAAGCGGAAGTTCCGGAA+TGG	-	chr4.2:6251266-6251285	MS.gene93204:CDS	55.0%
	TCCATTCCGGAACTTCCGCT+TGG	+	chr4.2:6251270-6251289	None:intergenic	55.0%
	GCGGAAGTTCCGGAATGGAC+TGG	-	chr4.2:6251271-6251290	MS.gene93204:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	6251067	6251582	6251067	ID=MS.gene93204
chr4.2	mRNA	6251067	6251582	6251067	ID=MS.gene93204.t1;Parent=MS.gene93204
chr4.2	exon	6251067	6251582	6251067	ID=MS.gene93204.t1.exon1;Parent=MS.gene93204.t1
chr4.2	CDS	6251067	6251582	6251067	ID=cds.MS.gene93204.t1;Parent=MS.gene93204.t1

Gene Sequence

>MS.gene93204

ATGGAGTCAGAAATATGTCCTCTGGAGAAAACAACAGCCAAACCAGACGTCGTCACTGAAAAGAAAAAATCAACTGCCAAACCAGATGCAACTGAGAAGAGAAAATCAACTGCCAACCCAGATATTACTGAAAAGAGAAAATCTGAGCCATCTATGGATGATGATTTTCGTGAAAAGCTTGTTTCTGTAGGTCACCTTTTCCAAGCGGAAGTTCCGGAATGGACTGGTGTAGTTTCTGAGAGTGACTCTAAATGGTTAGGCACACAAGTATGGCCTGTTAAAGATGATTCAAAACCTACTACCGAAACAGATCTTGTTGGGAGAGGAAGACGAGGAAAGTGTAGCTGCAACGTTCAAGGTTCTGTTGACTGTGTCAGATTTCATATTGCTGAGAACAGGATGAAGCTGAAGCTTGAATTGCATTCCGTGTTTTATCATTGGGGATTTGATAAAATGGTGAGGAAGTTTCACTTCGATGGACAGCTGATGAAGAAAAGAGATTTAAGGATGCAATGA

Protein sequence

>MS.gene93204.t1

MESEICPLEKTTAKPDVVTEKKKSTAKPDATEKRKSTANPDITEKRKSEPSMDDDFREKLVSVGHLFQAEVPEWTGVVSESDSKWLGTQVWPVKDDSKPTTETDLVGRGRRGKCSCNVQGSVDCVRFHIAENRMKLKLELHSVFYHWGFDKMVRKFHFDGQLMKKRDLRMQ