AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98141


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98141.t1 MTR_4g005440 97.794 136 2 1 1 135 1 136 3.67e-94 268
MS.gene98141.t1 MTR_3g108730 51.471 136 50 2 1 135 1 121 7.06e-37 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98141.t1 AT1G16810 59.722 144 49 3 1 135 1 144 3.20e-45 144
MS.gene98141.t1 AT1G16810 59.722 144 49 3 1 135 1 144 3.20e-45 144
MS.gene98141.t1 AT1G16810 59.722 144 49 3 1 135 1 144 3.20e-45 144

Find 29 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTTACTTCTTGATCATT+AGG 0.263557 4.1:-89191606 None:intergenic
CGCTCATGTTTGCGAGATAT+TGG 0.302036 4.1:-89191785 None:intergenic
AATCATCCAGGACCAACTTT+AGG 0.309675 4.1:-89191822 None:intergenic
GTGAGATTTATTAGAAATCT+TGG 0.347606 4.1:-89191739 None:intergenic
CGAGATATTGGTTGAAATCC+TGG 0.357664 4.1:-89191773 None:intergenic
GCAACTTGATGTTCATAGAT+TGG 0.407231 4.1:+89191716 MS.gene98141:CDS
GGAAAAGCATTGGATGTTGC+TGG 0.421175 4.1:+89190554 MS.gene98141:CDS
ATGATGGGAGTGAATTGAGC+AGG 0.471811 4.1:+89191625 MS.gene98141:CDS
CATTATGATATTCCTAAAGT+TGG 0.473152 4.1:+89191810 MS.gene98141:CDS
AATGATCAAGAAGTAAATGA+TGG 0.482714 4.1:+89191609 MS.gene98141:CDS
GATATTCCTAAAGTTGGTCC+TGG 0.485583 4.1:+89191816 MS.gene98141:CDS
TCTATGTACCGCCTCTCTGC+TGG 0.489804 4.1:-89191684 None:intergenic
ATCTCACCGCGATAGAATCC+AGG 0.518832 4.1:+89191755 MS.gene98141:CDS
GAAGCTGAAGGGAAAAGCAT+TGG 0.531649 4.1:+89190544 MS.gene98141:CDS
ACTGAAGATGAAATCTCAGC+AGG 0.540124 4.1:+89190632 MS.gene98141:CDS
AGATGATCATCATAATGAGC+AGG 0.545898 4.1:-89191657 None:intergenic
AACAACTCCTTGAATCATCC+AGG 0.551401 4.1:-89191834 None:intergenic
TCGGCATATGAGAATGTTGT+TGG 0.556165 4.1:+89190512 MS.gene98141:CDS
TGATGGGAGTGAATTGAGCA+GGG 0.558875 4.1:+89191626 MS.gene98141:CDS
ATGATCAAGAAGTAAATGAT+GGG 0.571574 4.1:+89191610 MS.gene98141:CDS
GAGGCGGTACATAGAACAGA+GGG 0.618835 4.1:+89191692 MS.gene98141:CDS
ATCTTACACCAGCAGAGAGG+CGG 0.643614 4.1:+89191676 MS.gene98141:CDS
GCATATGAGAATGTTGTTGG+TGG 0.647733 4.1:+89190515 MS.gene98141:CDS
TGAAATCCTGGATTCTATCG+CGG 0.661087 4.1:-89191761 None:intergenic
AGAGGCGGTACATAGAACAG+AGG 0.666218 4.1:+89191691 MS.gene98141:CDS
TGGTGGTAAACTGAAGCTGA+AGG 0.678860 4.1:+89190532 MS.gene98141:CDS
AAAGCATTGGATGTTGCTGG+CGG 0.694873 4.1:+89190557 MS.gene98141:CDS
GGTGGTAAACTGAAGCTGAA+GGG 0.696896 4.1:+89190533 MS.gene98141:CDS
ATCATCTTACACCAGCAGAG+AGG 0.709383 4.1:+89191673 MS.gene98141:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATTATGTTCAAGTA+AGG - chr4.1:89190997-89191016 None:intergenic 15.0%
!! TAAATATATTGCACAATTTA+TGG - chr4.1:89191528-89191547 None:intergenic 15.0%
!!! CATAGTATTATGTTTTAAAA+TGG + chr4.1:89190746-89190765 MS.gene98141:intron 15.0%
!!! TCAACAATAAATATCATTTT+CGG + chr4.1:89191061-89191080 MS.gene98141:intron 15.0%
!!! TTTTTTACTGAAAATCATAA+GGG + chr4.1:89191011-89191030 MS.gene98141:intron 15.0%
!!! TTTTTTTACTGAAAATCATA+AGG + chr4.1:89191010-89191029 MS.gene98141:intron 15.0%
!! AACTAAAACCAAAAAGATTA+AGG - chr4.1:89191503-89191522 None:intergenic 20.0%
!! ATTATAGTAAATGAGTATCT+GGG + chr4.1:89190928-89190947 MS.gene98141:intron 20.0%
!! GAGTATTATAACAATATGAA+TGG + chr4.1:89191295-89191314 MS.gene98141:intron 20.0%
!! TTTCTCATCATAATTTCTTT+GGG - chr4.1:89190862-89190881 None:intergenic 20.0%
!!! GATTTTGAAAGAAAAAGAAA+AGG - chr4.1:89191450-89191469 None:intergenic 20.0%
!!! TTATGTTTTAAAATGGTCAA+TGG + chr4.1:89190753-89190772 MS.gene98141:intron 20.0%
!!! TTTTCTCATCATAATTTCTT+TGG - chr4.1:89190863-89190882 None:intergenic 20.0%
! AAAAGATTAAGGATCTGTTT+CGG - chr4.1:89191492-89191511 None:intergenic 25.0%
! AATGATCAAGAAGTAAATGA+TGG + chr4.1:89191609-89191628 MS.gene98141:CDS 25.0%
! ATGATCAAGAAGTAAATGAT+GGG + chr4.1:89191610-89191629 MS.gene98141:CDS 25.0%
! CATTATGATATTCCTAAAGT+TGG + chr4.1:89191810-89191829 MS.gene98141:CDS 25.0%
! GATTATAGTAAATGAGTATC+TGG + chr4.1:89190927-89190946 MS.gene98141:intron 25.0%
! GTGAGATTTATTAGAAATCT+TGG - chr4.1:89191742-89191761 None:intergenic 25.0%
! TAATACAAAATCCCCTAATT+TGG - chr4.1:89191361-89191380 None:intergenic 25.0%
! TCATTTACTTCTTGATCATT+AGG - chr4.1:89191609-89191628 None:intergenic 25.0%
! TTGAAAGAAAAAGAAAAGGA+AGG - chr4.1:89191446-89191465 None:intergenic 25.0%
!! AAACAGATCCTTAATCTTTT+TGG + chr4.1:89191492-89191511 MS.gene98141:intron 25.0%
!! ATAAATATCATTTTCGGCAA+TGG + chr4.1:89191067-89191086 MS.gene98141:intron 25.0%
!! TAAATATCATTTTCGGCAAT+GGG + chr4.1:89191068-89191087 MS.gene98141:intron 25.0%
!!! TTTTTGGTTTACATTTCCTA+AGG + chr4.1:89191145-89191164 MS.gene98141:intron 25.0%
AAAGAAAAAGAAAAGGAAGG+AGG - chr4.1:89191443-89191462 None:intergenic 30.0%
CATAATTTCTTTGGGTCAAA+AGG - chr4.1:89190854-89190873 None:intergenic 30.0%
CCACCTACATTAAATACAAA+TGG - chr4.1:89191564-89191583 None:intergenic 30.0%
CCATTTGTATTTAATGTAGG+TGG + chr4.1:89191561-89191580 MS.gene98141:intron 30.0%
GAAGGACTCAAAAAATGTAT+TGG - chr4.1:89191339-89191358 None:intergenic 30.0%
GTAAGATGTAACAAGAATCA+AGG - chr4.1:89190723-89190742 None:intergenic 30.0%
TAACAAGAATCAAGGCAAAT+AGG - chr4.1:89190715-89190734 None:intergenic 30.0%
TCAAATAACCTAGATTGCAA+TGG - chr4.1:89190909-89190928 None:intergenic 30.0%
! TGTATGAATTTTGCAAGAAC+AGG + chr4.1:89190883-89190902 MS.gene98141:intron 30.0%
! TTTGTAGTCACTGTTCTTTT+TGG + chr4.1:89191129-89191148 MS.gene98141:intron 30.0%
!! GTGCCATTTGTATTTAATGT+AGG + chr4.1:89191558-89191577 MS.gene98141:intron 30.0%
!! TTTTGAGTCCTTCCAAATTA+GGG + chr4.1:89191346-89191365 MS.gene98141:intron 30.0%
!!! TTTTTGAGTCCTTCCAAATT+AGG + chr4.1:89191345-89191364 MS.gene98141:intron 30.0%
AATCAACTACGTTGCATAAC+AGG - chr4.1:89191110-89191129 None:intergenic 35.0%
ACAAAATCCCCTAATTTGGA+AGG - chr4.1:89191357-89191376 None:intergenic 35.0%
AGATGATCATCATAATGAGC+AGG - chr4.1:89191660-89191679 None:intergenic 35.0%
GCAACTTGATGTTCATAGAT+TGG + chr4.1:89191716-89191735 MS.gene98141:CDS 35.0%
GGACTCAAAAAATGTATTGG+AGG - chr4.1:89191336-89191355 None:intergenic 35.0%
TTTGAGTCCTTCCAAATTAG+GGG + chr4.1:89191347-89191366 MS.gene98141:intron 35.0%
AAAGAAAAGGAAGGAGGAAG+TGG - chr4.1:89191437-89191456 None:intergenic 40.0%
ACTGAAGATGAAATCTCAGC+AGG + chr4.1:89190632-89190651 MS.gene98141:CDS 40.0%
CTGAAAATCATAAGGGACAG+TGG + chr4.1:89191018-89191037 MS.gene98141:intron 40.0%
GCATATGAGAATGTTGTTGG+TGG + chr4.1:89190515-89190534 MS.gene98141:CDS 40.0%
TAAGGATCTGTTTCGGAGTT+TGG - chr4.1:89191485-89191504 None:intergenic 40.0%
TCGGCATATGAGAATGTTGT+TGG + chr4.1:89190512-89190531 MS.gene98141:CDS 40.0%
TGAAATCCTGGATTCTATCG+CGG - chr4.1:89191764-89191783 None:intergenic 40.0%
TTCTGTTTGATGTGCTGCAA+TGG + chr4.1:89190776-89190795 MS.gene98141:intron 40.0%
! CGAGATATTGGTTGAAATCC+TGG - chr4.1:89191776-89191795 None:intergenic 40.0%
! GATATTCCTAAAGTTGGTCC+TGG + chr4.1:89191816-89191835 MS.gene98141:CDS 40.0%
! GCAATGGGTTTCAGTAAACA+GGG + chr4.1:89191083-89191102 MS.gene98141:intron 40.0%
AAAAGGAAGGAGGAAGTGGA+GGG - chr4.1:89191433-89191452 None:intergenic 45.0%
ACATTTCCTAAGGCTCCGTT+CGG + chr4.1:89191155-89191174 MS.gene98141:intron 45.0%
AGAACAGGCCATTGCAATCT+AGG + chr4.1:89190898-89190917 MS.gene98141:intron 45.0%
ATCATCTTACACCAGCAGAG+AGG + chr4.1:89191673-89191692 MS.gene98141:CDS 45.0%
ATCTGTTTCGGAGTTTGGAG+GGG - chr4.1:89191480-89191499 None:intergenic 45.0%
CGCTCATGTTTGCGAGATAT+TGG - chr4.1:89191788-89191807 None:intergenic 45.0%
GATCTGTTTCGGAGTTTGGA+GGG - chr4.1:89191481-89191500 None:intergenic 45.0%
GGTGGTAAACTGAAGCTGAA+GGG + chr4.1:89190533-89190552 MS.gene98141:CDS 45.0%
TGATGGGAGTGAATTGAGCA+GGG + chr4.1:89191626-89191645 MS.gene98141:CDS 45.0%
TGGTGGTAAACTGAAGCTGA+AGG + chr4.1:89190532-89190551 MS.gene98141:CDS 45.0%
TGTGTGTGTGTGTGTGTTCA+TGG + chr4.1:89190675-89190694 MS.gene98141:intron 45.0%
! ATGATGGGAGTGAATTGAGC+AGG + chr4.1:89191625-89191644 MS.gene98141:CDS 45.0%
! GAAGCTGAAGGGAAAAGCAT+TGG + chr4.1:89190544-89190563 MS.gene98141:CDS 45.0%
! GGCAATGGGTTTCAGTAAAC+AGG + chr4.1:89191082-89191101 MS.gene98141:intron 45.0%
!! AAAGCATTGGATGTTGCTGG+CGG + chr4.1:89190557-89190576 MS.gene98141:CDS 45.0%
!! GGAAAAGCATTGGATGTTGC+TGG + chr4.1:89190554-89190573 MS.gene98141:CDS 45.0%
AGAGGCGGTACATAGAACAG+AGG + chr4.1:89191691-89191710 MS.gene98141:CDS 50.0%
AGGGAATAGAGGACTTCGGA+AGG - chr4.1:89191414-89191433 None:intergenic 50.0%
ATCTCACCGCGATAGAATCC+AGG + chr4.1:89191755-89191774 MS.gene98141:CDS 50.0%
ATCTTACACCAGCAGAGAGG+CGG + chr4.1:89191676-89191695 MS.gene98141:CDS 50.0%
CATTTCCTAAGGCTCCGTTC+GGG + chr4.1:89191156-89191175 MS.gene98141:intron 50.0%
GAAAAGGAAGGAGGAAGTGG+AGG - chr4.1:89191434-89191453 None:intergenic 50.0%
GAGGCGGTACATAGAACAGA+GGG + chr4.1:89191692-89191711 MS.gene98141:CDS 50.0%
GGAATAGAGGACTTCGGAAG+GGG - chr4.1:89191412-89191431 None:intergenic 50.0%
GGATCTGTTTCGGAGTTTGG+AGG - chr4.1:89191482-89191501 None:intergenic 50.0%
GGGAATAGAGGACTTCGGAA+GGG - chr4.1:89191413-89191432 None:intergenic 50.0%
GTGGAGGGAATAGAGGACTT+CGG - chr4.1:89191418-89191437 None:intergenic 50.0%
TTTCGGAGTTTGGAGGGGAA+GGG - chr4.1:89191475-89191494 None:intergenic 50.0%
!! AATGGATGGCTTTGTGAGGG+AGG + chr4.1:89191195-89191214 MS.gene98141:intron 50.0%
!! AGGGAATGGATGGCTTTGTG+AGG + chr4.1:89191191-89191210 MS.gene98141:intron 50.0%
!! ATGGATGGCTTTGTGAGGGA+GGG + chr4.1:89191196-89191215 MS.gene98141:intron 50.0%
!! GGGAATGGATGGCTTTGTGA+GGG + chr4.1:89191192-89191211 MS.gene98141:intron 50.0%
AAACTCCCGAACGGAGCCTT+AGG - chr4.1:89191164-89191183 None:intergenic 55.0%
ATAGAGGACTTCGGAAGGGG+AGG - chr4.1:89191409-89191428 None:intergenic 55.0%
GGAGGAAGTGGAGGGAATAG+AGG - chr4.1:89191425-89191444 None:intergenic 55.0%
GTTTCGGAGTTTGGAGGGGA+AGG - chr4.1:89191476-89191495 None:intergenic 55.0%
TAAGGCTCCGTTCGGGAGTT+TGG + chr4.1:89191163-89191182 MS.gene98141:intron 55.0%
TAGAGGACTTCGGAAGGGGA+GGG - chr4.1:89191408-89191427 None:intergenic 55.0%
TCTATGTACCGCCTCTCTGC+TGG - chr4.1:89191687-89191706 None:intergenic 55.0%
TTTGGAGGGGAGGGAATGGA+TGG + chr4.1:89191181-89191200 MS.gene98141:intron 55.0%
! GGCTTTGTGAGGGAGGGAAA+CGG + chr4.1:89191202-89191221 MS.gene98141:intron 55.0%
ACTTCGGAAGGGGAGGGCTT+TGG - chr4.1:89191402-89191421 None:intergenic 60.0%
CTCCCCTCCAAACTCCCGAA+CGG - chr4.1:89191173-89191192 None:intergenic 60.0%
CTCCGTTCGGGAGTTTGGAG+GGG + chr4.1:89191168-89191187 MS.gene98141:intron 60.0%
CTTCGGAAGGGGAGGGCTTT+GGG - chr4.1:89191401-89191420 None:intergenic 60.0%
GCTCCGTTCGGGAGTTTGGA+GGG + chr4.1:89191167-89191186 MS.gene98141:intron 60.0%
GGAGTTTGGAGGGGAGGGAA+TGG + chr4.1:89191177-89191196 MS.gene98141:intron 60.0%
GTTCGGGAGTTTGGAGGGGA+GGG + chr4.1:89191172-89191191 MS.gene98141:intron 60.0%
TTCGGAAGGGGAGGGCTTTG+GGG - chr4.1:89191400-89191419 None:intergenic 60.0%
CGGAGTTTGGAGGGGAAGGG+AGG - chr4.1:89191472-89191491 None:intergenic 65.0%
CGTTCGGGAGTTTGGAGGGG+AGG + chr4.1:89191171-89191190 MS.gene98141:intron 65.0%
GGCTCCGTTCGGGAGTTTGG+AGG + chr4.1:89191166-89191185 MS.gene98141:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 89190509 89191842 89190509 ID=MS.gene98141
chr4.1 mRNA 89190509 89191842 89190509 ID=MS.gene98141.t1;Parent=MS.gene98141
chr4.1 exon 89190509 89190653 89190509 ID=MS.gene98141.t1.exon1;Parent=MS.gene98141.t1
chr4.1 CDS 89190509 89190653 89190509 ID=cds.MS.gene98141.t1;Parent=MS.gene98141.t1
chr4.1 exon 89191580 89191842 89191580 ID=MS.gene98141.t1.exon2;Parent=MS.gene98141.t1
chr4.1 CDS 89191580 89191842 89191580 ID=cds.MS.gene98141.t1;Parent=MS.gene98141.t1
Gene Sequence

>MS.gene98141

ATGTCGGCATATGAGAATGTTGTTGGTGGTAAACTGAAGCTGAAGGGAAAAGCATTGGATGTTGCTGGCGGAATCAAGAAGAAGAAAAAGAACAAAAGAAATCAACAACAGTTTTTGCAAGCAACTGAAGATGAAATCTCAGCAGGTTAGTATCTCAGTGTGTGTGTGTGTGTGTGTGTGTGTTCATGGTCGTAGCTACATAACCTATTTGCCTTGATTCTTGTTACATCTTACAATCATAGTATTATGTTTTAAAATGGTCAATGGTTCTGTTTGATGTGCTGCAATGGTTTGATGAACATGTTACTGCTACTCTAGTTATTTGTTATGTAAGATGTTTTGCCTTTTGACCCAAAGAAATTATGATGAGAAAATGTATGAATTTTGCAAGAACAGGCCATTGCAATCTAGGTTATTTGATTATAGTAAATGAGTATCTGGGTGAACTCAATAATATTATGAATTTTGAATTAGTGCACTTCTCACCTTACTTGAACATAATTTTTTTACTGAAAATCATAAGGGACAGTGGACATTGTTTTATGACAATAGTCAACAATAAATATCATTTTCGGCAATGGGTTTCAGTAAACAGGGACCTGTTATGCAACGTAGTTGATTTTGTAGTCACTGTTCTTTTTGGTTTACATTTCCTAAGGCTCCGTTCGGGAGTTTGGAGGGGAGGGAATGGATGGCTTTGTGAGGGAGGGAAACGGATAGAAATTTGAGACAACGATTTTGTAGAGTGATAAGAGAATGCTCGTGTTTAATATATTTTAAATTTTAGAGTATTATAACAATATGAATGGCTTTGAAGAATTTATCCTCCAATACATTTTTTGAGTCCTTCCAAATTAGGGGATTTTGTATTATGATGAAAAATAAACTCCCCAAAGCCCTCCCCTTCCGAAGTCCTCTATTCCCTCCACTTCCTCCTTCCTTTTCTTTTTCTTTCAAAATCCTCCCTTCCCCTCCAAACTCCGAAACAGATCCTTAATCTTTTTGGTTTTAGTTCACCATAAATTGTGCAATATATTTACTTATAGTTTGTGCCATTTGTATTTAATGTAGGTGGAAGTACAGAACAAGCTAAAGATCCTAATGATCAAGAAGTAAATGATGGGAGTGAATTGAGCAGGGAAGAGAAGCCTGCTCATTATGATGATCATCTTACACCAGCAGAGAGGCGGTACATAGAACAGAGGGAGCAACTTGATGTTCATAGATTGGCCAAGATTTCTAATAAATCTCACCGCGATAGAATCCAGGATTTCAACCAATATCTCGCAAACATGAGCGAGCATTATGATATTCCTAAAGTTGGTCCTGGATGA

Protein sequence

>MS.gene98141.t1

MSAYENVVGGKLKLKGKALDVAGGIKKKKKNKRNQQQFLQATEDEISAGGSTEQAKDPNDQEVNDGSELSREEKPAHYDDHLTPAERRYIEQREQLDVHRLAKISNKSHRDRIQDFNQYLANMSEHYDIPKVGPG