AlfalfaGEDB | MsG0280008720.01

M. sativa cultivar ZhongmuNo.1 / MsG0280008720.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008720.01.T01	ACL97383.1	96.825	189	6	0	1	189	1096	1284	3.38E-124	390

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008720.01.T01	P10978	59.259	189	77	0	1	189	1119	1307	9.75E-79	258

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008720.01.T01	B8YLY3	96.825	189	6	0	1	189	1096	1284	1.61e-124	390

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048058.01	MsG0280008720.01	0.801243	9.869694e-49	4.690464e-46
MsG0180000101.01	MsG0280008720.01	0.802716	4.916964e-49	2.425749e-46
MsG0180000494.01	MsG0280008720.01	0.802100	6.584494e-49	3.197721e-46
MsG0180000555.01	MsG0280008720.01	0.835686	1.452014e-56	1.789005e-53
MsG0180000585.01	MsG0280008720.01	0.801048	1.081951e-48	5.116797e-46
MsG0180000741.01	MsG0280008720.01	0.825445	4.669401e-54	4.256643e-51
MsG0180000797.01	MsG0280008720.01	0.801626	8.241088e-49	3.954165e-46
MsG0180000821.01	MsG0280008720.01	0.825925	3.592048e-54	3.320046e-51
MsG0180000855.01	MsG0280008720.01	0.800087	1.698696e-48	7.839501e-46
MsG0180001294.01	MsG0280008720.01	0.810566	1.081719e-50	6.542620e-48
MsG0180001922.01	MsG0280008720.01	0.828365	9.362534e-55	9.286048e-52
MsG0180002185.01	MsG0280008720.01	0.805732	1.158157e-49	6.174236e-47
MsG0180003031.01	MsG0280008720.01	0.800489	1.406960e-48	6.559695e-46
MsG0180003190.01	MsG0280008720.01	0.804269	2.342538e-49	1.202550e-46
MsG0180003303.01	MsG0280008720.01	0.815497	8.968510e-52	6.193774e-49
MsG0180003744.01	MsG0280008720.01	0.805999	1.017860e-49	5.463404e-47
MsG0180003807.01	MsG0280008720.01	0.807250	5.539336e-50	3.070354e-47
MsG0180003977.01	MsG0280008720.01	0.811630	6.361157e-51	3.957340e-48
MsG0180004372.01	MsG0280008720.01	0.817640	2.969551e-52	2.174569e-49
MsG0180004598.01	MsG0280008720.01	0.811572	6.546398e-51	4.066573e-48
MsG0180004689.01	MsG0280008720.01	0.822502	2.288658e-53	1.919170e-50
MsG0180005354.01	MsG0280008720.01	0.805313	1.418139e-49	7.478566e-47
MsG0180005398.01	MsG0280008720.01	0.806933	6.466866e-50	3.554956e-47
MsG0180005443.01	MsG0280008720.01	0.806217	9.154880e-50	4.941381e-47
MsG0180005445.01	MsG0280008720.01	0.804022	2.636388e-49	1.344935e-46
MsG0180005451.01	MsG0280008720.01	0.808421	3.123221e-50	1.785006e-47
MsG0180005765.01	MsG0280008720.01	0.824505	7.782926e-54	6.907084e-51
MsG0180005773.01	MsG0280008720.01	0.805228	1.477029e-49	7.772115e-47
MsG0180005944.01	MsG0280008720.01	0.828301	9.700965e-55	9.603542e-52
MsG0180006050.01	MsG0280008720.01	0.806784	6.951151e-50	3.806510e-47
MsG0280006462.01	MsG0280008720.01	0.805035	1.621080e-49	8.487996e-47
MsG0280006728.01	MsG0280008720.01	0.809021	2.324528e-50	1.349632e-47
MsG0280006906.01	MsG0280008720.01	0.855738	5.158385e-62	1.202837e-58
MsG0280006935.01	MsG0280008720.01	0.843724	1.174876e-58	1.854820e-55
MsG0280007048.01	MsG0280008720.01	0.809458	1.873173e-50	1.100381e-47
MsG0280007564.01	MsG0280008720.01	0.801922	7.163112e-49	3.462982e-46
MsG0280007585.01	MsG0280008720.01	0.854236	1.408310e-61	3.124802e-58
MsG0280007710.01	MsG0280008720.01	0.801191	1.011796e-48	4.801920e-46
MsG0280007775.01	MsG0280008720.01	0.800396	1.469931e-48	6.837110e-46
MsG0280007984.01	MsG0280008720.01	0.802372	5.787586e-49	2.830472e-46
MsG0280008295.01	MsG0280008720.01	0.830393	3.011845e-55	3.169135e-52
MsG0280008502.01	MsG0280008720.01	0.846349	2.297198e-59	3.942518e-56
MsG0280008720.01	MsG0280008970.01	0.806156	9.433610e-50	5.083662e-47
MsG0280008720.01	MsG0280010416.01	0.800073	1.710266e-48	7.890008e-46
MsG0280008720.01	MsG0280010447.01	0.827969	1.166382e-54	1.143639e-51
MsG0280008720.01	MsG0280010614.01	0.809648	1.705334e-50	1.006921e-47
MsG0280008720.01	MsG0280010816.01	0.836305	1.011442e-56	1.269768e-53
MsG0280008720.01	MsG0280010976.01	0.825278	5.115406e-54	4.641501e-51
MsG0280008720.01	MsG0280011043.01	0.811665	6.248796e-51	3.891035e-48
MsG0280008720.01	MsG0280011235.01	0.805780	1.131580e-49	6.039730e-47
MsG0280008720.01	MsG0280011293.01	0.817652	2.950305e-52	2.161196e-49
MsG0280008720.01	MsG0280011334.01	0.821323	4.291330e-53	3.480075e-50
MsG0280008720.01	MsG0280011410.01	0.810779	9.729418e-51	5.918458e-48
MsG0280008720.01	MsG0380011663.01	0.803136	4.026642e-49	2.007788e-46
MsG0280008720.01	MsG0380011729.01	0.824783	6.695367e-54	5.990073e-51
MsG0280008720.01	MsG0380011754.01	0.808847	2.532984e-50	1.463843e-47
MsG0280008720.01	MsG0380012020.01	0.840859	6.746480e-58	9.736657e-55
MsG0280008720.01	MsG0380012458.01	0.820843	5.537756e-53	4.431375e-50
MsG0280008720.01	MsG0380014576.01	0.804757	1.853253e-49	9.634877e-47
MsG0280008720.01	MsG0380014913.01	0.804390	2.210638e-49	1.138383e-46
MsG0280008720.01	MsG0380015607.01	0.800819	1.204843e-48	5.664522e-46
MsG0280008720.01	MsG0380015614.01	0.828983	6.638891e-55	6.703512e-52
MsG0280008720.01	MsG0380016227.01	0.835964	1.234469e-56	1.533567e-53
MsG0280008720.01	MsG0380016752.01	0.813615	2.339648e-51	1.535297e-48
MsG0280008720.01	MsG0380016971.01	0.828640	8.032759e-55	8.031359e-52
MsG0280008720.01	MsG0380017210.01	0.816283	5.990226e-52	4.226841e-49
MsG0280008720.01	MsG0380017375.01	0.806136	9.524463e-50	5.129987e-47
MsG0280008720.01	MsG0380017403.01	0.810550	1.090265e-50	6.591355e-48
MsG0280008720.01	MsG0380017604.01	0.808564	2.911100e-50	1.669956e-47
MsG0280008720.01	MsG0480018117.01	0.800361	1.494384e-48	6.944788e-46
MsG0280008720.01	MsG0480018462.01	0.819655	1.036359e-52	8.023639e-50
MsG0280008720.01	MsG0480018479.01	0.803419	3.518409e-49	1.767210e-46
MsG0280008720.01	MsG0480018640.01	0.817418	3.331158e-52	2.424704e-49
MsG0280008720.01	MsG0480019289.01	0.822712	2.045228e-53	1.725281e-50
MsG0280008720.01	MsG0480019772.01	0.812618	3.871927e-51	2.473580e-48
MsG0280008720.01	MsG0480020242.01	0.804544	2.052680e-49	1.061241e-46
MsG0280008720.01	MsG0480020331.01	0.804987	1.659079e-49	8.676362e-47
MsG0280008720.01	MsG0480020540.01	0.815433	9.270292e-52	6.390510e-49
MsG0280008720.01	MsG0480020720.01	0.814534	1.467439e-51	9.872088e-49
MsG0280008720.01	MsG0480020923.01	0.800798	1.216847e-48	5.717924e-46
MsG0280008720.01	MsG0480021240.01	0.812127	4.956469e-51	3.125116e-48
MsG0280008720.01	MsG0480021426.01	0.801456	8.928738e-49	4.265901e-46
MsG0280008720.01	MsG0480021900.01	0.821695	3.520791e-53	2.885242e-50
MsG0280008720.01	MsG0480022026.01	0.820707	5.950455e-53	4.743375e-50
MsG0280008720.01	MsG0480022108.01	0.804994	1.653297e-49	8.647677e-47
MsG0280008720.01	MsG0480022318.01	0.831566	1.552775e-55	1.691532e-52
MsG0280008720.01	MsG0480022552.01	0.800681	1.285599e-48	6.023065e-46
MsG0280008720.01	MsG0480022597.01	0.801024	1.094102e-48	5.171175e-46
MsG0280008720.01	MsG0480022678.01	0.818632	1.771435e-52	1.333048e-49
MsG0280008720.01	MsG0480023032.01	0.800305	1.534151e-48	7.119566e-46
MsG0280008720.01	MsG0480023224.01	0.823252	1.530678e-53	1.311006e-50
MsG0280008720.01	MsG0480023245.01	0.839096	1.943815e-57	2.656359e-54
MsG0280008720.01	MsG0480023412.01	0.819340	1.222423e-52	9.380402e-50
MsG0280008720.01	MsG0480023517.01	0.817779	2.763010e-52	2.031088e-49
MsG0280008720.01	MsG0480023554.01	0.811271	7.610550e-51	4.690160e-48
MsG0280008720.01	MsG0480023659.01	0.800691	1.279526e-48	5.996144e-46
MsG0280008720.01	MsG0580024205.01	0.811840	5.723300e-51	3.580598e-48
MsG0280008720.01	MsG0580024374.01	0.809913	1.495639e-50	8.892511e-48
MsG0280008720.01	MsG0580024733.01	0.807279	5.461507e-50	3.029545e-47
MsG0280008720.01	MsG0580024960.01	0.823817	1.129143e-53	9.827807e-51
MsG0280008720.01	MsG0580024996.01	0.803348	3.639231e-49	1.824485e-46
MsG0280008720.01	MsG0580025008.01	0.803290	3.741292e-49	1.872893e-46
MsG0280008720.01	MsG0580025009.01	0.801487	8.796443e-49	4.206138e-46
MsG0280008720.01	MsG0580025769.01	0.815652	8.285808e-52	5.746541e-49
MsG0280008720.01	MsG0580025907.01	0.815599	8.510905e-52	5.894128e-49
MsG0280008720.01	MsG0580029629.01	0.817096	3.937778e-52	2.840874e-49
MsG0280008720.01	MsG0580029658.01	0.810098	1.364403e-50	8.151602e-48
MsG0280008720.01	MsG0580029762.01	0.810341	1.209605e-50	7.272538e-48
MsG0280008720.01	MsG0680030321.01	0.823620	1.255750e-53	1.086861e-50
MsG0280008720.01	MsG0680030684.01	0.829688	4.474164e-55	4.613146e-52
MsG0280008720.01	MsG0680030685.01	0.816034	6.807219e-52	4.770678e-49
MsG0280008720.01	MsG0680031301.01	0.811068	8.423799e-51	5.163771e-48
MsG0280008720.01	MsG0680031538.01	0.815738	7.924852e-52	5.509378e-49
MsG0280008720.01	MsG0680034037.01	0.803731	3.031268e-49	1.534792e-46
MsG0280008720.01	MsG0680034067.01	0.808403	3.150502e-50	1.799783e-47
MsG0280008720.01	MsG0680034757.01	0.831542	1.573814e-55	1.713287e-52
MsG0280008720.01	MsG0780036548.01	0.812223	4.724152e-51	2.986498e-48
MsG0280008720.01	MsG0780036609.01	0.809176	2.153879e-50	1.255802e-47
MsG0280008720.01	MsG0780036630.01	0.821589	3.726169e-53	3.044131e-50
MsG0280008720.01	MsG0780036633.01	0.828594	8.241276e-55	8.228669e-52
MsG0280008720.01	MsG0780036635.01	0.838961	2.107207e-57	2.867581e-54
MsG0280008720.01	MsG0780036773.01	0.802602	5.189029e-49	2.552489e-46
MsG0280008720.01	MsG0780036845.01	0.809142	2.189898e-50	1.275686e-47
MsG0280008720.01	MsG0780037287.01	0.812585	3.937262e-51	2.513109e-48
MsG0280008720.01	MsG0780037975.01	0.811479	6.857996e-51	4.249861e-48
MsG0280008720.01	MsG0780038092.01	0.810419	1.163363e-50	7.008870e-48
MsG0280008720.01	MsG0780038323.01	0.817363	3.428014e-52	2.491358e-49
MsG0280008720.01	MsG0780039777.01	0.844529	7.145811e-59	1.156992e-55
MsG0280008720.01	MsG0780040125.01	0.807781	4.274284e-50	2.402049e-47
MsG0280008720.01	MsG0780040176.01	0.827092	1.893896e-54	1.809965e-51
MsG0280008720.01	MsG0780040516.01	0.821476	3.956925e-53	3.222569e-50
MsG0280008720.01	MsG0780040679.01	0.803252	3.810100e-49	1.905505e-46
MsG0280008720.01	MsG0780040742.01	0.822828	1.922508e-53	1.627180e-50
MsG0280008720.01	MsG0780041161.01	0.817745	2.811273e-52	2.064717e-49
MsG0280008720.01	MsG0780041210.01	0.800568	1.355693e-48	6.333309e-46
MsG0280008720.01	MsG0780041368.01	0.811275	7.595528e-51	4.681285e-48
MsG0280008720.01	MsG0780041370.01	0.801802	7.583561e-49	3.655081e-46
MsG0280008720.01	MsG0780041469.01	0.822726	2.030731e-53	1.713748e-50
MsG0280008720.01	MsG0780041498.01	0.819489	1.130870e-52	8.714917e-50
MsG0280008720.01	MsG0880043059.01	0.810988	8.764255e-51	5.361843e-48
MsG0280008720.01	MsG0880043116.01	0.839803	1.273369e-57	1.778363e-54
MsG0280008720.01	MsG0880043162.01	0.812155	4.886422e-51	3.083356e-48
MsG0280008720.01	MsG0880043341.01	0.827741	1.323496e-54	1.288954e-51
MsG0280008720.01	MsG0880043489.01	0.803336	3.659881e-49	1.834311e-46
MsG0280008720.01	MsG0880043532.01	0.816827	4.523670e-52	3.239694e-49
MsG0280008720.01	MsG0880043533.01	0.810292	1.239271e-50	7.441206e-48
MsG0280008720.01	MsG0880043804.01	0.814364	1.599636e-51	1.071196e-48
MsG0280008720.01	MsG0880044732.01	0.801526	8.637558e-49	4.134163e-46
MsG0280008720.01	MsG0880045106.01	0.827537	1.480793e-54	1.433625e-51
MsG0280008720.01	MsG0880045914.01	0.819084	1.398299e-52	1.065500e-49
MsG0280008720.01	MsG0880046047.01	0.819021	1.444917e-52	1.099240e-49
MsG0280008720.01	MsG0880046113.01	0.808178	3.517973e-50	1.997899e-47
MsG0280008720.01	MsG0880046244.01	0.824396	8.258213e-54	7.305443e-51
MsG0280008720.01	MsG0880046560.01	0.821461	3.988947e-53	3.247198e-50
MsG0280008720.01	MsG0880046561.01	0.833558	4.977533e-56	5.752963e-53
MsG0280008720.01	MsG0880047391.01	0.801820	7.517289e-49	3.624874e-46
MsG0280008720.01	MsG0880047590.01	0.822596	2.177006e-53	1.830575e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008720.01.T01	MTR_6g472230	43.684	190	104	3	1	188	1	189	7.07e-48	167
MsG0280008720.01.T01	MTR_3g093120	38.378	185	111	3	7	189	326	509	4.78e-41	145
MsG0280008720.01.T01	MTR_3g022740	33.880	183	75	3	6	188	14	150	6.13e-29	113
MsG0280008720.01.T01	MTR_8g059225	38.462	104	64	0	68	171	71	174	8.23e-26	98.2
MsG0280008720.01.T01	MTR_3g448730	38.462	104	64	0	86	189	101	204	1.66e-25	99.0
MsG0280008720.01.T01	MTR_3g452820	33.133	166	73	3	7	172	34	161	7.21e-23	90.9
MsG0280008720.01.T01	MTR_7g026095	34.711	121	78	1	7	126	39	159	9.83e-20	85.5
MsG0280008720.01.T01	MTR_6g033925	39.091	110	63	2	1	109	32	138	3.09e-18	79.7
MsG0280008720.01.T01	MTR_2g075320	28.649	185	101	4	7	190	114	268	1.66e-17	79.0
MsG0280008720.01.T01	MTR_6g060522	38.000	100	61	1	94	192	3	102	2.26e-17	76.6
MsG0280008720.01.T01	MTR_8g011410	32.414	145	76	3	23	165	815	939	9.98e-17	78.2
MsG0280008720.01.T01	MTR_7g056567	36.559	93	58	1	100	191	24	116	5.00e-16	72.4
MsG0280008720.01.T01	MTR_1g051400	35.556	90	57	1	37	125	73	162	2.22e-15	70.9
MsG0280008720.01.T01	MTR_0046s0060	28.289	152	77	3	7	157	12	132	8.45e-15	70.1
MsG0280008720.01.T01	MTR_2g017850	27.481	131	82	1	15	145	188	305	5.49e-14	69.7
MsG0280008720.01.T01	MTR_2g460640	42.254	71	40	1	4	73	32	102	6.19e-14	66.6
MsG0280008720.01.T01	MTR_0303s0010	41.791	67	39	0	52	118	98	164	4.29e-12	62.0
MsG0280008720.01.T01	MTR_6g035385	37.000	100	48	2	59	158	94	178	4.34e-12	62.4
MsG0280008720.01.T01	MTR_7g056447	39.344	61	37	0	129	189	212	272	1.72e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008720.01.T01	AT4G23160	40.828	169	97	3	1	167	386	553	1.38e-31	120
MsG0280008720.01.T01	AT4G23160	40.828	169	97	3	1	167	386	553	1.38e-31	120

Find 48 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGAGTGGTTAGTCGGTTTA+TGG	0.227904	2:-37281281	MsG0280008720.01.T01:CDS
AAGCATGCAAGGAAGCTATT+TGG	0.306678	2:-37280994	MsG0280008720.01.T01:CDS
GGTTATGTGTTCACGCTTGC+AGG	0.309619	2:-37281097	MsG0280008720.01.T01:CDS
TATTTGGATGCAAAGGTTAA+TGG	0.314202	2:-37280978	MsG0280008720.01.T01:CDS
AGAATCATGAGGTACATTAA+AGG	0.348284	2:-37281223	MsG0280008720.01.T01:CDS
TAGTCGGTTTATGGCGGATC+CGG	0.362622	2:-37281272	MsG0280008720.01.T01:CDS
TCAGGTGTTGCGGTATGTTT+CGG	0.376448	2:-37281196	MsG0280008720.01.T01:CDS
CCCACTGCTTGTGCAATGTC+TGG	0.411743	2:+37281301	None:intergenic
AGCTACAACACTTTGTAACT+TGG	0.417141	2:+37281054	None:intergenic
TACATCTGCTAGGTTATCAT+TGG	0.421897	2:+37280784	None:intergenic
GTTCGAGAAGTAGTGGAGGA+AGG	0.424752	2:-37280833	MsG0280008720.01.T01:CDS
AATAGCTTCCTTGCATGCTT+GGG	0.428550	2:+37280997	None:intergenic
AAATAGCTTCCTTGCATGCT+TGG	0.431915	2:+37280996	None:intergenic
AGTAGTGGAGGAAGGGAGTG+TGG	0.445509	2:-37280825	MsG0280008720.01.T01:CDS
TTCGAGAAGTAGTGGAGGAA+GGG	0.460754	2:-37280832	MsG0280008720.01.T01:CDS
GTCTGGTCTTGTACATATCA+TGG	0.492315	2:+37281318	None:intergenic
GATAAAAGAAAATCTACTAC+TGG	0.492564	2:-37281118	MsG0280008720.01.T01:CDS
CGGATCCGGGTGAAGAGCAT+TGG	0.508764	2:-37281258	MsG0280008720.01.T01:CDS
TTGCAGGAGGAGCTGTAAGT+TGG	0.514152	2:-37281081	MsG0280008720.01.T01:CDS
CAAAGGTTAATGGAGGAACT+CGG	0.514212	2:-37280968	MsG0280008720.01.T01:CDS
GAGGATCAGAGTTAACTGTC+AGG	0.516487	2:-37281174	MsG0280008720.01.T01:CDS
AGTCGGTTTATGGCGGATCC+GGG	0.519661	2:-37281271	MsG0280008720.01.T01:CDS
AACAACAGAAGCTGAGTACA+TGG	0.521429	2:-37281026	MsG0280008720.01.T01:CDS
TTCGTCCTTGAATGAAAAGC+TGG	0.527181	2:+37280881	None:intergenic
AGGTACATTAAAGGAACCTC+AGG	0.532112	2:-37281214	MsG0280008720.01.T01:CDS
TGTCATTAATTACATCTGCT+AGG	0.545890	2:+37280774	None:intergenic
ACAAACTTGTCAGCGTTGAT+CGG	0.548205	2:+37280748	None:intergenic
CATTGCACAAGCAGTGGGAG+TGG	0.556767	2:-37281296	MsG0280008720.01.T01:CDS
ACCAGACATTGCACAAGCAG+TGG	0.557126	2:-37281302	MsG0280008720.01.T01:CDS
CATTCAAGGACGAAGCACAT+TGG	0.563949	2:-37280872	MsG0280008720.01.T01:CDS
GGAAGCTACCCAAGCATGCA+AGG	0.566934	2:-37281005	MsG0280008720.01.T01:CDS
AAGCAGTGGGAGTGGTTAGT+CGG	0.571285	2:-37281288	MsG0280008720.01.T01:CDS
AGAGTGCCTTGCACATTGCA+AGG	0.576127	2:-37280907	MsG0280008720.01.T01:CDS
AGGAAGCTATTTGGATGCAA+AGG	0.578784	2:-37280985	MsG0280008720.01.T01:CDS
AGGATCAGAGTTAACTGTCA+GGG	0.582959	2:-37281173	MsG0280008720.01.T01:CDS
GGAGGAACTCGGGCACAAGC+AGG	0.583526	2:-37280957	MsG0280008720.01.T01:CDS
TAAAGGAACCTCAGGTGTTG+CGG	0.594156	2:-37281206	MsG0280008720.01.T01:CDS
GCATTCCAATGCTCTTCACC+CGG	0.606618	2:+37281253	None:intergenic
TATGTGTTCACGCTTGCAGG+AGG	0.611970	2:-37281094	MsG0280008720.01.T01:CDS
GGTGTTGCGGTATGTTTCGG+AGG	0.612838	2:-37281193	MsG0280008720.01.T01:CDS
CCAGACATTGCACAAGCAGT+GGG	0.614822	2:-37281301	MsG0280008720.01.T01:CDS
AGTGGTTAGTCGGTTTATGG+CGG	0.616807	2:-37281278	MsG0280008720.01.T01:CDS
TTGGATGCAAAGGTTAATGG+AGG	0.617483	2:-37280975	MsG0280008720.01.T01:CDS
TGGATTCCTTGCAATGTGCA+AGG	0.617592	2:+37280901	None:intergenic
AAAGGTTAATGGAGGAACTC+GGG	0.623045	2:-37280967	MsG0280008720.01.T01:CDS
GAAACATACCGCAACACCTG+AGG	0.666786	2:+37281198	None:intergenic
GGATCAGAGTTAACTGTCAG+GGG	0.668527	2:-37281172	MsG0280008720.01.T01:CDS
ATGCTGTTAAAAGAATCATG+AGG	0.723101	2:-37281234	MsG0280008720.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GATAAAAGAAAATCTACTAC+TGG	-	Chr2:37280964-37280983	MsG0280008720.01.T01:CDS	25.0%
	AGAATCATGAGGTACATTAA+AGG	-	Chr2:37280859-37280878	MsG0280008720.01.T01:CDS	30.0%
	ATGCTGTTAAAAGAATCATG+AGG	-	Chr2:37280848-37280867	MsG0280008720.01.T01:CDS	30.0%
	TATTTGGATGCAAAGGTTAA+TGG	-	Chr2:37281104-37281123	MsG0280008720.01.T01:CDS	30.0%
	TGTCATTAATTACATCTGCT+AGG	+	Chr2:37281311-37281330	None:intergenic	30.0%
!!!	TATGTTGATTCAGATTTTGC+AGG	-	Chr2:37280934-37280953	MsG0280008720.01.T01:CDS	30.0%
	AGCTACAACACTTTGTAACT+TGG	+	Chr2:37281031-37281050	None:intergenic	35.0%
	TACATCTGCTAGGTTATCAT+TGG	+	Chr2:37281301-37281320	None:intergenic	35.0%
!	TCATTTTGTTCGAGAAGTAG+TGG	-	Chr2:37281242-37281261	MsG0280008720.01.T01:CDS	35.0%
	AAAGGTTAATGGAGGAACTC+GGG	-	Chr2:37281115-37281134	MsG0280008720.01.T01:CDS	40.0%
	AAATAGCTTCCTTGCATGCT+TGG	+	Chr2:37281089-37281108	None:intergenic	40.0%
	AACAACAGAAGCTGAGTACA+TGG	-	Chr2:37281056-37281075	MsG0280008720.01.T01:CDS	40.0%
	AAGCATGCAAGGAAGCTATT+TGG	-	Chr2:37281088-37281107	MsG0280008720.01.T01:CDS	40.0%
	AATAGCTTCCTTGCATGCTT+GGG	+	Chr2:37281088-37281107	None:intergenic	40.0%
	AGGAAGCTATTTGGATGCAA+AGG	-	Chr2:37281097-37281116	MsG0280008720.01.T01:CDS	40.0%
	AGGATCAGAGTTAACTGTCA+GGG	-	Chr2:37280909-37280928	MsG0280008720.01.T01:CDS	40.0%
	AGGTACATTAAAGGAACCTC+AGG	-	Chr2:37280868-37280887	MsG0280008720.01.T01:CDS	40.0%
	CAAAGGTTAATGGAGGAACT+CGG	-	Chr2:37281114-37281133	MsG0280008720.01.T01:CDS	40.0%
	TTCGTCCTTGAATGAAAAGC+TGG	+	Chr2:37281204-37281223	None:intergenic	40.0%
	TTGGATGCAAAGGTTAATGG+AGG	-	Chr2:37281107-37281126	MsG0280008720.01.T01:CDS	40.0%
!	GGAATCCAGCTTTTCATTCA+AGG	-	Chr2:37281196-37281215	MsG0280008720.01.T01:CDS	40.0%
!	TTTTGTTCGAGAAGTAGTGG+AGG	-	Chr2:37281245-37281264	MsG0280008720.01.T01:CDS	40.0%
!!	GTCTGGTCTTGTACATATCA+TGG	+	Chr2:37280767-37280786	None:intergenic	40.0%
	CATTCAAGGACGAAGCACAT+TGG	-	Chr2:37281210-37281229	MsG0280008720.01.T01:CDS	45.0%
	GAGGATCAGAGTTAACTGTC+AGG	-	Chr2:37280908-37280927	MsG0280008720.01.T01:CDS	45.0%
	GGATCAGAGTTAACTGTCAG+GGG	-	Chr2:37280910-37280929	MsG0280008720.01.T01:CDS	45.0%
	TGGATTCCTTGCAATGTGCA+AGG	+	Chr2:37281184-37281203	None:intergenic	45.0%
!	AGTGGTTAGTCGGTTTATGG+CGG	-	Chr2:37280804-37280823	MsG0280008720.01.T01:CDS	45.0%
!	TAAAGGAACCTCAGGTGTTG+CGG	-	Chr2:37280876-37280895	MsG0280008720.01.T01:CDS	45.0%
!	TCAGGTGTTGCGGTATGTTT+CGG	-	Chr2:37280886-37280905	MsG0280008720.01.T01:CDS	45.0%
!	TTCGAGAAGTAGTGGAGGAA+GGG	-	Chr2:37281250-37281269	MsG0280008720.01.T01:CDS	45.0%
	AAGCAGTGGGAGTGGTTAGT+CGG	-	Chr2:37280794-37280813	MsG0280008720.01.T01:CDS	50.0%
	ACCAGACATTGCACAAGCAG+TGG	-	Chr2:37280780-37280799	MsG0280008720.01.T01:CDS	50.0%
	CCAGACATTGCACAAGCAGT+GGG	-	Chr2:37280781-37280800	MsG0280008720.01.T01:CDS	50.0%
	GAAACATACCGCAACACCTG+AGG	+	Chr2:37280887-37280906	None:intergenic	50.0%
	GCATTCCAATGCTCTTCACC+CGG	+	Chr2:37280832-37280851	None:intergenic	50.0%
	GGTTATGTGTTCACGCTTGC+AGG	-	Chr2:37280985-37281004	MsG0280008720.01.T01:CDS	50.0%
	TATGTGTTCACGCTTGCAGG+AGG	-	Chr2:37280988-37281007	MsG0280008720.01.T01:CDS	50.0%
	TTGCAGGAGGAGCTGTAAGT+TGG	-	Chr2:37281001-37281020	MsG0280008720.01.T01:CDS	50.0%
!	AGAGTGCCTTGCACATTGCA+AGG	-	Chr2:37281175-37281194	MsG0280008720.01.T01:CDS	50.0%
!	GGGAGTGGTTAGTCGGTTTA+TGG	-	Chr2:37280801-37280820	MsG0280008720.01.T01:CDS	50.0%
!	GTTCGAGAAGTAGTGGAGGA+AGG	-	Chr2:37281249-37281268	MsG0280008720.01.T01:CDS	50.0%
!!	TAGTCGGTTTATGGCGGATC+CGG	-	Chr2:37280810-37280829	MsG0280008720.01.T01:CDS	50.0%
	CATTGCACAAGCAGTGGGAG+TGG	-	Chr2:37280786-37280805	MsG0280008720.01.T01:CDS	55.0%
	CCCACTGCTTGTGCAATGTC+TGG	+	Chr2:37280784-37280803	None:intergenic	55.0%
	GGAAGCTACCCAAGCATGCA+AGG	-	Chr2:37281077-37281096	MsG0280008720.01.T01:CDS	55.0%
!	AGTAGTGGAGGAAGGGAGTG+TGG	-	Chr2:37281257-37281276	MsG0280008720.01.T01:CDS	55.0%
!	GGTGTTGCGGTATGTTTCGG+AGG	-	Chr2:37280889-37280908	MsG0280008720.01.T01:CDS	55.0%
!!	AGTCGGTTTATGGCGGATCC+GGG	-	Chr2:37280811-37280830	MsG0280008720.01.T01:CDS	55.0%
!	CGGATCCGGGTGAAGAGCAT+TGG	-	Chr2:37280824-37280843	MsG0280008720.01.T01:CDS	60.0%
	GGAGGAACTCGGGCACAAGC+AGG	-	Chr2:37281125-37281144	MsG0280008720.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	37280757	37281347	37280757	ID=MsG0280008720.01;Name=MsG0280008720.01
Chr2	mRNA	37280757	37281347	37280757	ID=MsG0280008720.01.T01;Parent=MsG0280008720.01;Name=MsG0280008720.01.T01;_AED=0.33;_eAED=0.33;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|196
Chr2	exon	37280757	37281347	37280757	ID=MsG0280008720.01.T01:exon:6358;Parent=MsG0280008720.01.T01
Chr2	CDS	37280757	37281347	37280757	ID=MsG0280008720.01.T01:cds;Parent=MsG0280008720.01.T01

Gene Sequence

>MsG0280008720.01.T01

ATGTATGCCATGATATGTACAAGACCAGACATTGCACAAGCAGTGGGAGTGGTTAGTCGGTTTATGGCGGATCCGGGTGAAGAGCATTGGAATGCTGTTAAAAGAATCATGAGGTACATTAAAGGAACCTCAGGTGTTGCGGTATGTTTCGGAGGATCAGAGTTAACTGTCAGGGGTTATGTTGATTCAGATTTTGCAGGTGATCATGATAAAAGAAAATCTACTACTGGTTATGTGTTCACGCTTGCAGGAGGAGCTGTAAGTTGGTTATCCAAGTTACAAAGTGTTGTAGCTCTGTCAACAACAGAAGCTGAGTACATGGAAGCTACCCAAGCATGCAAGGAAGCTATTTGGATGCAAAGGTTAATGGAGGAACTCGGGCACAAGCAGGAACAAATTACTGTGTATTGTGACAATCAGAGTGCCTTGCACATTGCAAGGAATCCAGCTTTTCATTCAAGGACGAAGCACATTGGCGTTCAATATCATTTTGTTCGAGAAGTAGTGGAGGAAGGGAGTGTGGATATGAAAAAGATTCACACCAATGATAACCTAGCAGATGTAATTAATGACAAAGCCGATCAACGCTGA

Protein sequence

>MsG0280008720.01.T01

MYAMICTRPDIAQAVGVVSRFMADPGEEHWNAVKRIMRYIKGTSGVAVCFGGSELTVRGYVDSDFAGDHDKRKSTTGYVFTLAGGAVSWLSKLQSVVALSTTEAEYMEATQACKEAIWMQRLMEELGHKQEQITVYCDNQSALHIARNPAFHSRTKHIGVQYHFVREVVEEGSVDMKKIHTNDNLADVINDKADQR*