AlfalfaGEDB | MsG0380016223.01

M. sativa cultivar ZhongmuNo.1 / MsG0380016223.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016223.01.T01	ACL97386.1	100	96	0	0	1	96	293	388	1.96E-58	204

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047825.01	MsG0380016223.01	0.809531	1.807409e-50	1.063818e-47
MsG0080048177.01	MsG0380016223.01	0.813775	2.157677e-51	1.421945e-48
MsG0080048178.01	MsG0380016223.01	0.836621	8.407567e-57	1.065256e-53
MsG0080048765.01	MsG0380016223.01	0.808739	2.671143e-50	1.539250e-47
MsG0080048830.01	MsG0380016223.01	0.815596	8.526806e-52	5.904496e-49
MsG0080048982.01	MsG0380016223.01	0.803000	4.295600e-49	2.134435e-46
MsG0180000157.01	MsG0380016223.01	0.811851	5.692373e-51	3.562269e-48
MsG0180000263.01	MsG0380016223.01	0.800512	1.392141e-48	6.494237e-46
MsG0180000494.01	MsG0380016223.01	0.812549	4.009581e-51	2.556931e-48
MsG0180000555.01	MsG0380016223.01	0.801199	1.007832e-48	4.784120e-46
MsG0180000585.01	MsG0380016223.01	0.801675	8.049798e-49	3.867295e-46
MsG0180000741.01	MsG0380016223.01	0.821895	3.165740e-53	2.609083e-50
MsG0180000797.01	MsG0380016223.01	0.846045	2.779212e-59	4.723308e-56
MsG0180000821.01	MsG0380016223.01	0.815510	8.911828e-52	6.156587e-49
MsG0180000855.01	MsG0380016223.01	0.811546	6.631850e-51	4.116977e-48
MsG0180001043.01	MsG0380016223.01	0.812115	4.987949e-51	3.143781e-48
MsG0180001312.01	MsG0380016223.01	0.820260	7.533926e-53	5.931929e-50
MsG0180001570.01	MsG0380016223.01	0.819237	1.290728e-52	9.876911e-50
MsG0180001712.01	MsG0380016223.01	0.816413	5.601089e-52	3.966085e-49
MsG0180001911.01	MsG0380016223.01	0.801832	7.473944e-49	3.605121e-46
MsG0180001922.01	MsG0380016223.01	0.852879	3.453892e-61	7.322762e-58
MsG0180002185.01	MsG0380016223.01	0.830380	3.034837e-55	3.192045e-52
MsG0180002300.01	MsG0380016223.01	0.800179	1.626953e-48	7.525798e-46
MsG0180002709.01	MsG0380016223.01	0.822920	1.829976e-53	1.552833e-50
MsG0180003118.01	MsG0380016223.01	-0.819590	1.072196e-52	8.286173e-50
MsG0180003190.01	MsG0380016223.01	0.827804	1.277749e-54	1.246642e-51
MsG0180003252.01	MsG0380016223.01	0.822106	2.829128e-53	2.345537e-50
MsG0180003303.01	MsG0380016223.01	0.831183	1.928039e-55	2.076394e-52
MsG0180003744.01	MsG0380016223.01	0.804805	1.811277e-49	9.428346e-47
MsG0180004162.01	MsG0380016223.01	0.808057	3.733801e-50	2.113510e-47
MsG0180004437.01	MsG0380016223.01	0.811784	5.886127e-51	3.677018e-48
MsG0180004598.01	MsG0380016223.01	0.809317	2.008350e-50	1.175378e-47
MsG0180004689.01	MsG0380016223.01	0.802248	6.140120e-49	2.993238e-46
MsG0180004730.01	MsG0380016223.01	0.803521	3.351063e-49	1.687631e-46
MsG0180005125.01	MsG0380016223.01	0.821466	3.978620e-53	3.239225e-50
MsG0180005354.01	MsG0380016223.01	0.810076	1.379327e-50	8.235970e-48
MsG0180005398.01	MsG0380016223.01	0.803955	2.722449e-49	1.386468e-46
MsG0180005765.01	MsG0380016223.01	0.810156	1.325756e-50	7.932549e-48
MsG0180005773.01	MsG0380016223.01	0.817928	2.556709e-52	1.887170e-49
MsG0180005944.01	MsG0380016223.01	0.823941	1.056320e-53	9.225756e-51
MsG0180005949.01	MsG0380016223.01	0.825087	5.674826e-54	5.121473e-51
MsG0180005999.01	MsG0380016223.01	0.807590	4.691707e-50	2.623433e-47
MsG0180006016.01	MsG0380016223.01	0.819370	1.203432e-52	9.242650e-50
MsG0180006023.01	MsG0380016223.01	0.806824	6.818157e-50	3.737556e-47
MsG0180006050.01	MsG0380016223.01	0.834793	2.440262e-56	2.926506e-53
MsG0280006367.01	MsG0380016223.01	0.817803	2.728917e-52	2.007365e-49
MsG0280010784.01	MsG0380016223.01	0.811500	6.785607e-51	4.207445e-48
MsG0280010816.01	MsG0380016223.01	0.820156	7.961761e-53	6.250887e-50
MsG0280010901.01	MsG0380016223.01	0.811504	6.772291e-51	4.199598e-48
MsG0280010976.01	MsG0380016223.01	0.817653	2.949278e-52	2.160472e-49
MsG0280011131.01	MsG0380016223.01	0.835653	1.480452e-56	1.822021e-53
MsG0280011235.01	MsG0380016223.01	0.819544	1.098315e-52	8.477066e-50
MsG0280011410.01	MsG0380016223.01	0.825878	3.687276e-54	3.403436e-51
MsG0380011663.01	MsG0380016223.01	0.805697	1.177749e-49	6.272950e-47
MsG0380011729.01	MsG0380016223.01	0.823137	1.628488e-53	1.390409e-50
MsG0380011754.01	MsG0380016223.01	0.813091	3.050980e-51	1.973545e-48
MsG0380012020.01	MsG0380016223.01	0.812592	3.922545e-51	2.504215e-48
MsG0380012124.01	MsG0380016223.01	0.800272	1.557666e-48	7.222474e-46
MsG0380012458.01	MsG0380016223.01	0.848236	6.970979e-60	1.270877e-56
MsG0380014561.01	MsG0380016223.01	0.802412	5.679329e-49	2.780268e-46
MsG0380014913.01	MsG0380016223.01	0.840181	1.014618e-57	1.433916e-54
MsG0380015607.01	MsG0380016223.01	0.813706	2.234909e-51	1.470159e-48
MsG0380015614.01	MsG0380016223.01	0.834152	3.535888e-56	4.159726e-53
MsG0380015771.01	MsG0380016223.01	0.805291	1.432713e-49	7.551218e-47
MsG0380015931.01	MsG0380016223.01	0.811015	8.648379e-51	5.294544e-48
MsG0380016223.01	MsG0380016227.01	0.824964	6.067095e-54	5.456224e-51
MsG0380016223.01	MsG0380016752.01	0.801958	7.042727e-49	3.407868e-46
MsG0380016223.01	MsG0380017003.01	0.800031	1.743622e-48	8.035351e-46
MsG0380016223.01	MsG0380017158.01	0.813286	2.764362e-51	1.797751e-48
MsG0380016223.01	MsG0380017210.01	0.806933	6.466965e-50	3.555006e-47
MsG0380016223.01	MsG0380017226.01	0.811899	5.557973e-51	3.482534e-48
MsG0380016223.01	MsG0380017403.01	0.828765	7.495317e-55	7.520545e-52
MsG0380016223.01	MsG0380017512.01	0.822582	2.193037e-53	1.843291e-50
MsG0380016223.01	MsG0380017604.01	0.822696	2.063288e-53	1.739730e-50
MsG0380016223.01	MsG0380017914.01	-0.804674	1.928915e-49	1.000623e-46
MsG0380016223.01	MsG0480018321.01	0.811921	5.497860e-51	3.446829e-48
MsG0380016223.01	MsG0480018332.01	0.816211	6.216429e-52	4.377735e-49
MsG0380016223.01	MsG0480018460.01	0.839526	1.503472e-57	2.081738e-54
MsG0380016223.01	MsG0480018462.01	0.810805	9.600039e-51	5.843803e-48
MsG0380016223.01	MsG0480018635.01	0.802669	5.028550e-49	2.477700e-46
MsG0380016223.01	MsG0480019289.01	0.847207	1.338000e-59	2.360605e-56
MsG0380016223.01	MsG0480019493.01	0.806077	9.801017e-50	5.270957e-47
MsG0380016223.01	MsG0480020416.01	0.828423	9.064139e-55	9.005818e-52
MsG0380016223.01	MsG0480020445.01	0.821525	3.855009e-53	3.143755e-50
MsG0380016223.01	MsG0480020540.01	0.816281	5.994312e-52	4.229566e-49
MsG0380016223.01	MsG0480020719.01	0.803114	4.069083e-49	2.027766e-46
MsG0380016223.01	MsG0480020923.01	0.829469	5.057741e-55	5.181109e-52
MsG0380016223.01	MsG0480021034.01	0.826807	2.215202e-54	2.099410e-51
MsG0380016223.01	MsG0480021204.01	0.804826	1.792906e-49	9.337859e-47
MsG0380016223.01	MsG0480021240.01	0.822220	2.662254e-53	2.214400e-50
MsG0380016223.01	MsG0480021900.01	0.808693	2.732039e-50	1.572482e-47
MsG0380016223.01	MsG0480022108.01	0.814007	1.918339e-51	1.272222e-48
MsG0380016223.01	MsG0480022597.01	0.823252	1.530735e-53	1.311048e-50
MsG0380016223.01	MsG0480022598.01	0.810527	1.102915e-50	6.663673e-48
MsG0380016223.01	MsG0480022678.01	0.818797	1.624960e-52	1.228418e-49
MsG0380016223.01	MsG0480023032.01	0.820402	6.992188e-53	5.527259e-50
MsG0380016223.01	MsG0480023224.01	0.817080	3.969770e-52	2.862746e-49
MsG0380016223.01	MsG0480023245.01	0.800120	1.672475e-48	7.724964e-46
MsG0380016223.01	MsG0480023270.01	0.806628	7.499917e-50	4.090334e-47
MsG0380016223.01	MsG0480023417.01	0.802036	6.787753e-49	3.291058e-46
MsG0380016223.01	MsG0480023517.01	0.849780	2.595656e-60	4.975604e-57
MsG0380016223.01	MsG0480023554.01	0.812888	3.379950e-51	2.174638e-48
MsG0380016223.01	MsG0480023659.01	0.802214	6.237473e-49	3.038008e-46
MsG0380016223.01	MsG0480023923.01	0.804193	2.429015e-49	1.244522e-46
MsG0380016223.01	MsG0580024114.01	0.810224	1.281715e-50	7.682247e-48
MsG0380016223.01	MsG0580024581.01	0.841820	3.768442e-58	5.603423e-55
MsG0380016223.01	MsG0580024733.01	0.803207	3.892702e-49	1.944552e-46
MsG0380016223.01	MsG0580025278.01	0.862732	4.114087e-64	1.217354e-60
MsG0380016223.01	MsG0580025328.01	0.815287	9.988111e-52	6.857991e-49
MsG0380016223.01	MsG0580025453.01	0.831285	1.820878e-55	1.966882e-52
MsG0380016223.01	MsG0580025497.01	0.805018	1.634540e-49	8.554676e-47
MsG0380016223.01	MsG0580025769.01	0.822549	2.232335e-53	1.874537e-50
MsG0380016223.01	MsG0580025907.01	0.819322	1.234402e-52	9.467557e-50
MsG0380016223.01	MsG0580025922.01	0.802237	6.170122e-49	3.007007e-46
MsG0380016223.01	MsG0580026068.01	0.803436	3.489932e-49	1.753665e-46
MsG0380016223.01	MsG0580026595.01	0.800633	1.314974e-48	6.153336e-46
MsG0380016223.01	MsG0580026596.01	0.815145	1.073775e-51	7.343564e-49
MsG0380016223.01	MsG0580027034.01	0.809204	2.124478e-50	1.239622e-47
MsG0380016223.01	MsG0580027129.01	0.812530	4.048136e-51	2.580196e-48
MsG0380016223.01	MsG0580027628.01	0.812445	4.224335e-51	2.686587e-48
MsG0380016223.01	MsG0580028526.01	0.806717	7.183606e-50	3.926955e-47
MsG0380016223.01	MsG0580029639.01	0.812875	3.402338e-51	2.188173e-48
MsG0380016223.01	MsG0580029658.01	0.821065	4.921442e-53	3.962977e-50
MsG0380016223.01	MsG0680030321.01	0.800113	1.678635e-48	7.751755e-46
MsG0380016223.01	MsG0680030684.01	0.823388	1.422663e-53	1.223107e-50
MsG0380016223.01	MsG0680030841.01	0.827223	1.761712e-54	1.690128e-51
MsG0380016223.01	MsG0680031536.01	0.818252	2.159852e-52	1.608382e-49
MsG0380016223.01	MsG0680031538.01	0.814296	1.656048e-51	1.106966e-48
MsG0380016223.01	MsG0680032641.01	0.803171	3.958804e-49	1.975826e-46
MsG0380016223.01	MsG0680034450.01	0.816057	6.729494e-52	4.719140e-49
MsG0380016223.01	MsG0680034672.01	0.804493	2.104153e-49	1.086404e-46
MsG0380016223.01	MsG0680034757.01	0.810430	1.157330e-50	6.974516e-48
MsG0380016223.01	MsG0680035582.01	0.802012	6.866139e-49	3.326987e-46
MsG0380016223.01	MsG0780035958.01	0.810359	1.198562e-50	7.209765e-48
MsG0380016223.01	MsG0780036630.01	0.809746	1.625098e-50	9.620322e-48
MsG0380016223.01	MsG0780036633.01	0.838754	2.382885e-57	3.222468e-54
MsG0380016223.01	MsG0780036635.01	0.816222	6.180957e-52	4.354049e-49
MsG0380016223.01	MsG0780036845.01	0.800527	1.381958e-48	6.449286e-46
MsG0380016223.01	MsG0780037895.01	0.813418	2.585816e-51	1.687767e-48
MsG0380016223.01	MsG0780037975.01	0.803987	2.681926e-49	1.366940e-46
MsG0380016223.01	MsG0780038092.01	0.815253	1.016414e-51	6.971735e-49
MsG0380016223.01	MsG0780038231.01	0.800377	1.483059e-48	6.895055e-46
MsG0380016223.01	MsG0780038444.01	0.806320	8.709795e-50	4.713217e-47
MsG0380016223.01	MsG0780038836.01	0.804256	2.357124e-49	1.209611e-46
MsG0380016223.01	MsG0780039405.01	0.809954	1.465334e-50	8.721938e-48
MsG0380016223.01	MsG0780039452.01	0.815916	7.234566e-52	5.053431e-49
MsG0380016223.01	MsG0780039587.01	0.800301	1.536787e-48	7.131191e-46
MsG0380016223.01	MsG0780039875.01	0.805536	1.273028e-49	6.751873e-47
MsG0380016223.01	MsG0780039900.01	0.802983	4.330528e-49	2.150848e-46
MsG0380016223.01	MsG0780040125.01	0.827851	1.244836e-54	1.216344e-51
MsG0380016223.01	MsG0780040516.01	0.806290	8.837936e-50	4.778995e-47
MsG0380016223.01	MsG0780040679.01	0.844896	5.691315e-59	9.321433e-56
MsG0380016223.01	MsG0780040726.01	0.803779	2.962044e-49	1.501587e-46
MsG0380016223.01	MsG0780040742.01	0.836216	1.065766e-56	1.334409e-53
MsG0380016223.01	MsG0780040758.01	0.819090	1.393802e-52	1.062278e-49
MsG0380016223.01	MsG0780040929.01	0.824292	8.733179e-54	7.702729e-51
MsG0380016223.01	MsG0780041161.01	0.812423	4.271292e-51	2.714845e-48
MsG0380016223.01	MsG0780041370.01	0.817974	2.496503e-52	1.844903e-49
MsG0380016223.01	MsG0780041469.01	0.802056	6.723024e-49	3.261416e-46
MsG0380016223.01	MsG0780041754.01	0.805781	1.130996e-49	6.036744e-47
MsG0380016223.01	MsG0880042053.01	0.801597	8.353947e-49	4.005345e-46
MsG0380016223.01	MsG0880042329.01	0.808768	2.633555e-50	1.518748e-47
MsG0380016223.01	MsG0880042811.01	0.806586	7.655548e-50	4.170862e-47
MsG0380016223.01	MsG0880042965.01	0.804607	1.991543e-49	1.031325e-46
MsG0380016223.01	MsG0880043001.01	0.811523	6.710490e-51	4.163234e-48
MsG0380016223.01	MsG0880043059.01	0.805646	1.207170e-49	6.420881e-47
MsG0380016223.01	MsG0880043082.01	0.810189	1.304399e-50	7.811052e-48
MsG0380016223.01	MsG0880043288.01	0.813233	2.839233e-51	1.843690e-48
MsG0380016223.01	MsG0880043341.01	0.829515	4.931341e-55	5.058227e-52
MsG0380016223.01	MsG0880043835.01	0.803289	3.742674e-49	1.873534e-46
MsG0380016223.01	MsG0880044409.01	0.802360	5.822119e-49	2.846437e-46
MsG0380016223.01	MsG0880044546.01	0.835493	1.624746e-56	1.990099e-53
MsG0380016223.01	MsG0880045106.01	0.838848	2.253942e-57	3.056783e-54
MsG0380016223.01	MsG0880045165.01	0.811970	5.362399e-51	3.366591e-48
MsG0380016223.01	MsG0880045415.01	0.811198	7.892550e-51	4.854702e-48
MsG0380016223.01	MsG0880045914.01	0.826599	2.483619e-54	2.339664e-51
MsG0380016223.01	MsG0880046047.01	0.827487	1.522303e-54	1.471774e-51
MsG0380016223.01	MsG0880046058.01	0.812798	3.536988e-51	2.270057e-48
MsG0380016223.01	MsG0880046113.01	0.802868	4.574454e-49	2.265488e-46
MsG0380016223.01	MsG0880046209.01	0.821826	3.284973e-53	2.702008e-50
MsG0380016223.01	MsG0880046244.01	0.851136	1.080036e-60	2.164769e-57
MsG0380016223.01	MsG0880046329.01	0.827566	1.457664e-54	1.412389e-51
MsG0380016223.01	MsG0880046404.01	0.830233	3.295219e-55	3.451503e-52
MsG0380016223.01	MsG0880046733.01	0.809560	1.781857e-50	1.049576e-47
MsG0380016223.01	MsG0880047016.01	0.803035	4.223436e-49	2.100510e-46
MsG0380016223.01	MsG0880047019.01	0.813152	2.957631e-51	1.916379e-48
MsG0380016223.01	MsG0880047497.01	0.816570	5.165208e-52	3.673257e-49
MsG0380016223.01	MsG0880047590.01	0.829706	4.430681e-55	4.570726e-52
MsG0380016223.01	MsG0880047745.01	0.811120	8.208133e-51	5.038369e-48
MsG0280006462.01	MsG0380016223.01	0.810480	1.129064e-50	6.813384e-48
MsG0280006519.01	MsG0380016223.01	0.831982	1.225487e-55	1.351575e-52
MsG0280006595.01	MsG0380016223.01	0.817207	3.717277e-52	2.689977e-49
MsG0280006682.01	MsG0380016223.01	0.803149	4.000992e-49	1.995721e-46
MsG0280006728.01	MsG0380016223.01	0.834206	3.427034e-56	4.038412e-53
MsG0280006892.01	MsG0380016223.01	0.829970	3.820086e-55	3.970741e-52
MsG0280006906.01	MsG0380016223.01	0.801890	7.273158e-49	3.513540e-46
MsG0280006935.01	MsG0380016223.01	0.839082	1.960417e-57	2.677749e-54
MsG0280007048.01	MsG0380016223.01	0.808375	3.194350e-50	1.823505e-47
MsG0280007549.01	MsG0380016223.01	0.814598	1.419750e-51	9.567869e-49
MsG0280007585.01	MsG0380016223.01	0.800102	1.686884e-48	7.787854e-46
MsG0280007714.01	MsG0380016223.01	0.809941	1.475155e-50	8.777087e-48
MsG0280007775.01	MsG0380016223.01	0.825128	5.549842e-54	5.014241e-51
MsG0280007948.01	MsG0380016223.01	0.800407	1.462271e-48	6.803473e-46
MsG0280008286.01	MsG0380016223.01	0.813076	3.073245e-51	1.987211e-48
MsG0280008295.01	MsG0380016223.01	0.832505	9.100372e-56	1.019206e-52
MsG0280008414.01	MsG0380016223.01	0.801673	8.058380e-49	3.871213e-46
MsG0280008502.01	MsG0380016223.01	0.813008	3.180689e-51	2.053003e-48
MsG0280008907.01	MsG0380016223.01	0.807367	5.232250e-50	2.908870e-47
MsG0280009235.01	MsG0380016223.01	0.804272	2.339275e-49	1.200977e-46
MsG0280010416.01	MsG0380016223.01	0.820419	6.929632e-53	5.480422e-50
MsG0280010447.01	MsG0380016223.01	0.803589	3.243910e-49	1.636542e-46
MsG0280010531.01	MsG0380016223.01	0.813168	2.934252e-51	1.902027e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016223.01.T01	MTR_1g027280	32.292	96	65	0	1	96	117	212	9.33e-14	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 28 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGGAGTGCGTCATGTAAA+GGG	0.380784	3:+80611104	MsG0380016223.01.T01:CDS
TTTATTGTCTGTTGGACAAT+TGG	0.381122	3:+80611139	MsG0380016223.01.T01:CDS
ACCGACTCCAGCAATTTCTA+AGG	0.418839	3:-80611039	None:intergenic
ACATGACTCCACACCGAGAT+TGG	0.424052	3:+80610961	MsG0380016223.01.T01:CDS
GACCATGCCTTAGAAATTGC+TGG	0.432632	3:+80611032	MsG0380016223.01.T01:CDS
AAGAAGAATTTATTGTCTGT+TGG	0.439533	3:+80611131	MsG0380016223.01.T01:CDS
TACACTGATCCTTCCGAGAT+AGG	0.441988	3:-80610999	None:intergenic
CTCGGAAGGATCAGTGTACA+TGG	0.451780	3:+80611004	MsG0380016223.01.T01:CDS
GTTGGACAATTGGATGATCT+CGG	0.487496	3:+80611149	MsG0380016223.01.T01:CDS
ACAAGGAGTGCGTCATGTAA+AGG	0.489152	3:+80611103	MsG0380016223.01.T01:CDS
TGTAAGATCCACACTGAAAG+TGG	0.513302	3:+80611173	MsG0380016223.01.T01:CDS
CATAAGAGTAAAACCAATCT+CGG	0.529800	3:-80610974	None:intergenic
GCCTTAGAAATTGCTGGAGT+CGG	0.532564	3:+80611038	MsG0380016223.01.T01:CDS
GTAGTGAAAGGCAATCTTGT+GGG	0.540231	3:+80611206	MsG0380016223.01.T01:CDS
TTACTCTTATGAGCCTATCT+CGG	0.549243	3:+80610986	MsG0380016223.01.T01:CDS
CTCCAGCAATTTCTAAGGCA+TGG	0.552132	3:-80611034	None:intergenic
TTGGACAATTGGATGATCTC+GGG	0.567343	3:+80611150	MsG0380016223.01.T01:CDS
GGAATCTTGAAAGTAGTGAA+AGG	0.593190	3:+80611194	MsG0380016223.01.T01:CDS
ATCAGATTAAAGATGCATGA+TGG	0.595011	3:+80611065	MsG0380016223.01.T01:CDS
TCTTATGAGCCTATCTCGGA+AGG	0.598620	3:+80610990	MsG0380016223.01.T01:CDS
TCAAGATTCCACTTTCAGTG+TGG	0.613891	3:-80611181	None:intergenic
GATGTCTGGATCATGGACTC+AGG	0.621640	3:+80610927	None:intergenic
AGTAGTGAAAGGCAATCTTG+TGG	0.632415	3:+80611205	MsG0380016223.01.T01:CDS
TCGGAAGGATCAGTGTACAT+GGG	0.635842	3:+80611005	MsG0380016223.01.T01:CDS
CGGAAGGATCAGTGTACATG+GGG	0.640050	3:+80611006	MsG0380016223.01.T01:CDS
TCATGGACTCAGGTGCAACA+TGG	0.644772	3:+80610937	None:intergenic
AAGGAGTGCGTCATGTAAAG+GGG	0.650729	3:+80611105	MsG0380016223.01.T01:CDS
GAGTAAAACCAATCTCGGTG+TGG	0.676612	3:-80610969	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGAAGAATTTATTGTCTGT+TGG	+	Chr3:80611131-80611150	MsG0380016223.01.T01:CDS	25.0%
	ATCAGATTAAAGATGCATGA+TGG	+	Chr3:80611065-80611084	MsG0380016223.01.T01:CDS	30.0%
	CATAAGAGTAAAACCAATCT+CGG	-	Chr3:80610977-80610996	None:intergenic	30.0%
	TTTATTGTCTGTTGGACAAT+TGG	+	Chr3:80611139-80611158	MsG0380016223.01.T01:CDS	30.0%
	GGAATCTTGAAAGTAGTGAA+AGG	+	Chr3:80611194-80611213	MsG0380016223.01.T01:CDS	35.0%
	GGTACTGTTCGAAAAATACA+AGG	+	Chr3:80611086-80611105	MsG0380016223.01.T01:CDS	35.0%
	TTACTCTTATGAGCCTATCT+CGG	+	Chr3:80610986-80611005	MsG0380016223.01.T01:CDS	35.0%
	AGTAGTGAAAGGCAATCTTG+TGG	+	Chr3:80611205-80611224	MsG0380016223.01.T01:CDS	40.0%
	GTAGTGAAAGGCAATCTTGT+GGG	+	Chr3:80611206-80611225	MsG0380016223.01.T01:CDS	40.0%
	GTTGGACAATTGGATGATCT+CGG	+	Chr3:80611149-80611168	MsG0380016223.01.T01:CDS	40.0%
	TCAAGATTCCACTTTCAGTG+TGG	-	Chr3:80611184-80611203	None:intergenic	40.0%
	TGTAAGATCCACACTGAAAG+TGG	+	Chr3:80611173-80611192	MsG0380016223.01.T01:CDS	40.0%
	TTGGACAATTGGATGATCTC+GGG	+	Chr3:80611150-80611169	MsG0380016223.01.T01:CDS	40.0%
	GACCATGCCTTAGAAATTGC+TGG	+	Chr3:80611032-80611051	MsG0380016223.01.T01:CDS	45.0%
	GAGTAAAACCAATCTCGGTG+TGG	-	Chr3:80610972-80610991	None:intergenic	45.0%
	GCCTTAGAAATTGCTGGAGT+CGG	+	Chr3:80611038-80611057	MsG0380016223.01.T01:CDS	45.0%
	TACACTGATCCTTCCGAGAT+AGG	-	Chr3:80611002-80611021	None:intergenic	45.0%
	TCGGAAGGATCAGTGTACAT+GGG	+	Chr3:80611005-80611024	MsG0380016223.01.T01:CDS	45.0%
	TCTTATGAGCCTATCTCGGA+AGG	+	Chr3:80610990-80611009	MsG0380016223.01.T01:CDS	45.0%
!	AAGGAGTGCGTCATGTAAAG+GGG	+	Chr3:80611105-80611124	MsG0380016223.01.T01:CDS	45.0%
!	ACAAGGAGTGCGTCATGTAA+AGG	+	Chr3:80611103-80611122	MsG0380016223.01.T01:CDS	45.0%
!	ACCGACTCCAGCAATTTCTA+AGG	-	Chr3:80611042-80611061	None:intergenic	45.0%
!	CAAGGAGTGCGTCATGTAAA+GGG	+	Chr3:80611104-80611123	MsG0380016223.01.T01:CDS	45.0%
!!	CTCCAGCAATTTCTAAGGCA+TGG	-	Chr3:80611037-80611056	None:intergenic	45.0%
	ACATGACTCCACACCGAGAT+TGG	+	Chr3:80610961-80610980	MsG0380016223.01.T01:CDS	50.0%
	CGGAAGGATCAGTGTACATG+GGG	+	Chr3:80611006-80611025	MsG0380016223.01.T01:CDS	50.0%
	CTCGGAAGGATCAGTGTACA+TGG	+	Chr3:80611004-80611023	MsG0380016223.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	80610939	80611232	80610939	ID=MsG0380016223.01;Name=MsG0380016223.01
Chr3	mRNA	80610939	80611232	80610939	ID=MsG0380016223.01.T01;Parent=MsG0380016223.01;Name=MsG0380016223.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|97
Chr3	exon	80610939	80611232	80610939	ID=MsG0380016223.01.T01:exon:32865;Parent=MsG0380016223.01.T01
Chr3	CDS	80610939	80611232	80610939	ID=MsG0380016223.01.T01:cds;Parent=MsG0380016223.01.T01

Gene Sequence	>MsG0380016223.01.T01 ATGGACTCAGGTGCAACATGGCACATGACTCCACACCGAGATTGGTTTTACTCTTATGAGCCTATCTCGGAAGGATCAGTGTACATGGGGAATGACCATGCCTTAGAAATTGCTGGAGTCGGTACTATCAGATTAAAGATGCATGATGGTACTGTTCGAAAAATACAAGGAGTGCGTCATGTAAAGGGGTTGAAGAAGAATTTATTGTCTGTTGGACAATTGGATGATCTCGGGTGTAAGATCCACACTGAAAGTGGAATCTTGAAAGTAGTGAAAGGCAATCTTGTGGGATGA
Protein sequence	>MsG0380016223.01.T01 MDSGATWHMTPHRDWFYSYEPISEGSVYMGNDHALEIAGVGTIRLKMHDGTVRKIQGVRHVKGLKKNLLSVGQLDDLGCKIHTESGILKVVKGNLVG*