AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004442.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004442.01.T01 MTR_1g079890 99.187 123 1 0 1 123 1 123 7.37e-86 250
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004442.01.T01 AT4G12590 79.675 123 25 0 1 123 1 123 1.34e-69 209

Find 35 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGTTGTTATGGTTCTTAT+TGG 0.227769 1:+78025463 MsG0180004442.01.T01:CDS
TATCCCTTTATCCGTTGTTA+TGG 0.332373 1:+78025453 MsG0180004442.01.T01:CDS
AGAACCATAACAACGGATAA+AGG 0.351939 1:-78025457 None:intergenic
TAGAATAATGGCGGAAGATC+TGG 0.378579 1:+78025399 MsG0180004442.01.T01:exon
TGGATACTGCAATCAGAGAT+TGG 0.384123 1:+78025425 MsG0180004442.01.T01:CDS
CGGGCACGAAAAGCCTTAGA+AGG 0.386182 1:-78025696 None:intergenic
GGATTCGTGGCTTGGCCCTT+TGG 0.388165 1:-78026031 None:intergenic
CTATCGAAAGACGAGGTTGC+CGG 0.390954 1:+78025142 MsG0180004442.01.T01:five_prime_UTR
AATGGCGGAAGATCTGGTTT+TGG 0.395876 1:+78025405 MsG0180004442.01.T01:exon
GTGCTTGTGGATTCGTGGCT+TGG 0.403806 1:-78026039 None:intergenic
GAACCATAACAACGGATAAA+GGG 0.432462 1:-78025456 None:intergenic
GGATACTGCAATCAGAGATT+GGG 0.436107 1:+78025426 MsG0180004442.01.T01:CDS
ATCATATCCATGGCCATGTT+CGG 0.446386 1:-78026076 None:intergenic
TCTTTCAATGATTATACCTC+AGG 0.457257 1:+78026108 MsG0180004442.01.T01:CDS
ATTACAATAATAAAGCTTGC+GGG 0.463442 1:-78025715 None:intergenic
TCAGAGAACATTTGTGCTTG+TGG 0.484350 1:-78026052 None:intergenic
CATTTGTGCTTGTGGATTCG+TGG 0.484951 1:-78026044 None:intergenic
TTGGGAAGAACGCAGAAGCT+TGG 0.489162 1:-78025506 None:intergenic
CAAGCTTTATTATTGTAATG+AGG 0.490585 1:+78025719 MsG0180004442.01.T01:CDS
AGAGAAACAAGATCGAGAAC+TGG 0.497129 1:-78025185 None:intergenic
TGGTCTGTTATTCGTTCCAA+AGG 0.520176 1:+78026015 MsG0180004442.01.T01:CDS
CTCTGATCCGAACATGGCCA+TGG 0.554603 1:+78026069 MsG0180004442.01.T01:CDS
GATATCAAAATTGTCAGAGA+AGG 0.558054 1:+78025535 MsG0180004442.01.T01:CDS
GGTCTGTTATTCGTTCCAAA+GGG 0.570620 1:+78026016 MsG0180004442.01.T01:CDS
GATCGATTGTGAGACACAGC+CGG 0.576208 1:-78025161 None:intergenic
CATTACAATAATAAAGCTTG+CGG 0.576893 1:-78025716 None:intergenic
GGCAAGTTATGCTTAGAGCT+CGG 0.581999 1:+78025646 MsG0180004442.01.T01:intron
ATATCAAAATTGTCAGAGAA+GGG 0.586701 1:+78025536 MsG0180004442.01.T01:CDS
AATGTTCTCTGATCCGAACA+TGG 0.607119 1:+78026063 MsG0180004442.01.T01:CDS
ACCAATAAGAACCATAACAA+CGG 0.636744 1:-78025464 None:intergenic
CCACGATGTAGAATCTGATG+AGG 0.642648 1:-78025244 None:intergenic
GGGCACGAAAAGCCTTAGAA+GGG 0.660230 1:-78025695 None:intergenic
TGTTAAACTATCGAAAGACG+AGG 0.674238 1:+78025135 MsG0180004442.01.T01:five_prime_UTR
TAATCTATTCTGAACACCTG+AGG 0.692763 1:-78026124 None:intergenic
CCTCATCAGATTCTACATCG+TGG 0.717446 1:+78025244 MsG0180004442.01.T01:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTTATGTTTCTTATTT+AGG + Chr1:78025771-78025790 MsG0180004442.01.T01:intron 10.0%
!! TATTTAGGAAAAAAATGTAA+TGG + Chr1:78025786-78025805 MsG0180004442.01.T01:intron 15.0%
!!! ATTGTGTATTATTTTAGATT+TGG - Chr1:78025317-78025336 None:intergenic 15.0%
!!! TTGTGTATTATTTTAGATTT+GGG - Chr1:78025316-78025335 None:intergenic 15.0%
!!! TTTGTTAGATTTTAGAATAA+TGG + Chr1:78025387-78025406 MsG0180004442.01.T01:exon 15.0%
!!! TTTTTAATTTGATTATGTTG+TGG + Chr1:78025613-78025632 MsG0180004442.01.T01:intron 15.0%
!! AATTAACAACTTATTGTTTG+AGG + Chr1:78025825-78025844 MsG0180004442.01.T01:intron 20.0%
!! TGAAATTGAAATTAGAAACA+AGG - Chr1:78025284-78025303 None:intergenic 20.0%
!!! AAGTTTTTCAGTAAAACAAA+AGG + Chr1:78025066-78025085 MsG0180004442.01.T01:five_prime_UTR 20.0%
!!! CAATTTTTGTTAAAGGAAAA+TGG + Chr1:78025995-78026014 MsG0180004442.01.T01:intron 20.0%
!!! TGTTTGTCAATTTTTGTTAA+AGG + Chr1:78025988-78026007 MsG0180004442.01.T01:intron 20.0%
!!! TTTTATTCTAGTTTGATAGA+AGG + Chr1:78025746-78025765 MsG0180004442.01.T01:intron 20.0%
! ATATCAAAATTGTCAGAGAA+GGG + Chr1:78025536-78025555 MsG0180004442.01.T01:CDS 25.0%
! ATTACAATAATAAAGCTTGC+GGG - Chr1:78025718-78025737 None:intergenic 25.0%
! CATTACAATAATAAAGCTTG+CGG - Chr1:78025719-78025738 None:intergenic 25.0%
!! ATTTTTCTTCATCATATCCA+TGG - Chr1:78026089-78026108 None:intergenic 25.0%
!!! AATTTTGATATCAGGAGTTT+GGG - Chr1:78025527-78025546 None:intergenic 25.0%
!!! AGAAAACCTTTAAAACTCAT+CGG + Chr1:78025876-78025895 MsG0180004442.01.T01:intron 25.0%
!!! CAAGCTTTATTATTGTAATG+AGG + Chr1:78025719-78025738 MsG0180004442.01.T01:CDS 25.0%
!!! GTTAGATTTTAGAATAATGG+CGG + Chr1:78025390-78025409 MsG0180004442.01.T01:exon 25.0%
!!! TTATGTTGTGGTTTTAATAC+AGG + Chr1:78025625-78025644 MsG0180004442.01.T01:intron 25.0%
ACCAATAAGAACCATAACAA+CGG - Chr1:78025467-78025486 None:intergenic 30.0%
CATTTGTCTAACCAATTCAA+CGG + Chr1:78025956-78025975 MsG0180004442.01.T01:intron 30.0%
GATATCAAAATTGTCAGAGA+AGG + Chr1:78025535-78025554 MsG0180004442.01.T01:CDS 30.0%
TCTTTCAATGATTATACCTC+AGG + Chr1:78026108-78026127 MsG0180004442.01.T01:CDS 30.0%
! AAATGTAATGGCTTTTGTTG+TGG + Chr1:78025798-78025817 MsG0180004442.01.T01:intron 30.0%
! AGCTAATTTTATCCCTTCTA+AGG + Chr1:78025683-78025702 MsG0180004442.01.T01:CDS 30.0%
!! AATGTAATGGCTTTTGTTGT+GGG + Chr1:78025799-78025818 MsG0180004442.01.T01:intron 30.0%
!! CAATTTTGATATCAGGAGTT+TGG - Chr1:78025528-78025547 None:intergenic 30.0%
!! TAACTTCCGATGAGTTTTAA+AGG - Chr1:78025885-78025904 None:intergenic 30.0%
!! TCTCTGACAATTTTGATATC+AGG - Chr1:78025535-78025554 None:intergenic 30.0%
AGAACCATAACAACGGATAA+AGG - Chr1:78025460-78025479 None:intergenic 35.0%
AGGTGTTCAGAATAGATTAG+GGG + Chr1:78026128-78026147 MsG0180004442.01.T01:CDS 35.0%
CAATTAGTTCACCGTTGAAT+TGG - Chr1:78025970-78025989 None:intergenic 35.0%
CAGGTGTTCAGAATAGATTA+GGG + Chr1:78026127-78026146 MsG0180004442.01.T01:CDS 35.0%
GAACCATAACAACGGATAAA+GGG - Chr1:78025459-78025478 None:intergenic 35.0%
TAATCTATTCTGAACACCTG+AGG - Chr1:78026127-78026146 None:intergenic 35.0%
TATCCCTTTATCCGTTGTTA+TGG + Chr1:78025453-78025472 MsG0180004442.01.T01:CDS 35.0%
TCAGGTGTTCAGAATAGATT+AGG + Chr1:78026126-78026145 MsG0180004442.01.T01:CDS 35.0%
TCCGTTGTTATGGTTCTTAT+TGG + Chr1:78025463-78025482 MsG0180004442.01.T01:CDS 35.0%
TGTTAAACTATCGAAAGACG+AGG + Chr1:78025135-78025154 MsG0180004442.01.T01:five_prime_UTR 35.0%
AATGTTCTCTGATCCGAACA+TGG + Chr1:78026063-78026082 MsG0180004442.01.T01:CDS 40.0%
AGAGAAACAAGATCGAGAAC+TGG - Chr1:78025188-78025207 None:intergenic 40.0%
ATCATATCCATGGCCATGTT+CGG - Chr1:78026079-78026098 None:intergenic 40.0%
GGATACTGCAATCAGAGATT+GGG + Chr1:78025426-78025445 MsG0180004442.01.T01:CDS 40.0%
GGTCTGTTATTCGTTCCAAA+GGG + Chr1:78026016-78026035 MsG0180004442.01.T01:CDS 40.0%
TAACTCTATGACTCTGTCAC+AGG + Chr1:78025906-78025925 MsG0180004442.01.T01:intron 40.0%
TCAGAGAACATTTGTGCTTG+TGG - Chr1:78026055-78026074 None:intergenic 40.0%
TGGATACTGCAATCAGAGAT+TGG + Chr1:78025425-78025444 MsG0180004442.01.T01:CDS 40.0%
TGGTCTGTTATTCGTTCCAA+AGG + Chr1:78026015-78026034 MsG0180004442.01.T01:CDS 40.0%
! TAGAATAATGGCGGAAGATC+TGG + Chr1:78025399-78025418 MsG0180004442.01.T01:exon 40.0%
CATTTGTGCTTGTGGATTCG+TGG - Chr1:78026047-78026066 None:intergenic 45.0%
CCACGATGTAGAATCTGATG+AGG - Chr1:78025247-78025266 None:intergenic 45.0%
CCTCATCAGATTCTACATCG+TGG + Chr1:78025244-78025263 MsG0180004442.01.T01:five_prime_UTR 45.0%
GGCAAGTTATGCTTAGAGCT+CGG + Chr1:78025646-78025665 MsG0180004442.01.T01:intron 45.0%
!! AATGGCGGAAGATCTGGTTT+TGG + Chr1:78025405-78025424 MsG0180004442.01.T01:exon 45.0%
CTATCGAAAGACGAGGTTGC+CGG + Chr1:78025142-78025161 MsG0180004442.01.T01:five_prime_UTR 50.0%
GATCGATTGTGAGACACAGC+CGG - Chr1:78025164-78025183 None:intergenic 50.0%
GGGCACGAAAAGCCTTAGAA+GGG - Chr1:78025698-78025717 None:intergenic 50.0%
TTGGGAAGAACGCAGAAGCT+TGG - Chr1:78025509-78025528 None:intergenic 50.0%
CGGGCACGAAAAGCCTTAGA+AGG - Chr1:78025699-78025718 None:intergenic 55.0%
CTCTGATCCGAACATGGCCA+TGG + Chr1:78026069-78026088 MsG0180004442.01.T01:CDS 55.0%
GTGCTTGTGGATTCGTGGCT+TGG - Chr1:78026042-78026061 None:intergenic 55.0%
! GGATTCGTGGCTTGGCCCTT+TGG - Chr1:78026034-78026053 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 78025058 78026147 78025058 ID=MsG0180004442.01;Name=MsG0180004442.01
Chr1 mRNA 78025058 78026147 78025058 ID=MsG0180004442.01.T01;Parent=MsG0180004442.01;Name=MsG0180004442.01.T01;_AED=0.44;_eAED=0.46;_QI=227|1|0.75|1|0.66|0.5|4|0|127
Chr1 exon 78025058 78025265 78025058 ID=MsG0180004442.01.T01:exon:12828;Parent=MsG0180004442.01.T01
Chr1 exon 78025387 78025557 78025387 ID=MsG0180004442.01.T01:exon:12829;Parent=MsG0180004442.01.T01
Chr1 exon 78025647 78025740 78025647 ID=MsG0180004442.01.T01:exon:12830;Parent=MsG0180004442.01.T01
Chr1 exon 78026010 78026147 78026010 ID=MsG0180004442.01.T01:exon:12831;Parent=MsG0180004442.01.T01
Chr1 five_prime_UTR 78025058 78025265 78025058 ID=MsG0180004442.01.T01:five_prime_utr;Parent=MsG0180004442.01.T01
Chr1 five_prime_UTR 78025387 78025405 78025387 ID=MsG0180004442.01.T01:five_prime_utr;Parent=MsG0180004442.01.T01
Chr1 CDS 78025406 78025557 78025406 ID=MsG0180004442.01.T01:cds;Parent=MsG0180004442.01.T01
Chr1 CDS 78025647 78025740 78025647 ID=MsG0180004442.01.T01:cds;Parent=MsG0180004442.01.T01
Chr1 CDS 78026010 78026147 78026010 ID=MsG0180004442.01.T01:cds;Parent=MsG0180004442.01.T01
Gene Sequence

>MsG0180004442.01.T01

ATGGCGGAAGATCTGGTTTTGGATACTGCAATCAGAGATTGGGTTCTTATCCCTTTATCCGTTGTTATGGTTCTTATTGGTGTGCTTCGTCATATTGTTTCCAAGCTTCTGCGTTCTTCCCAAACTCCTGATATCAAAATTGTCAGAGAAGGGCAAGTTATGCTTAGAGCTCGGAATTTACGTGCTGCAGCTAATTTTATCCCTTCTAAGGCTTTTCGTGCCCGCAAGCTTTATTATTGTAATGAGGAAAATGGTCTGTTATTCGTTCCAAAGGGCCAAGCCACGAATCCACAAGCACAAATGTTCTCTGATCCGAACATGGCCATGGATATGATGAAGAAAAATCTTTCAATGATTATACCTCAGGTGTTCAGAATAGATTAG

Protein sequence

>MsG0180004442.01.T01

MAEDLVLDTAIRDWVLIPLSVVMVLIGVLRHIVSKLLRSSQTPDIKIVREGQVMLRARNLRAAANFIPSKAFRARKLYYCNEENGLLFVPKGQATNPQAQMFSDPNMAMDMMKKNLSMIIPQVFRID*