AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009356.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009356.01.T02 MTR_2g060830 68.376 117 5 1 1 85 1 117 2.23e-48 153
MsG0280009356.01.T02 MTR_8g464460 62.393 117 12 1 1 85 1 117 1.94e-43 140
MsG0280009356.01.T01 MTR_2g060830 68.376 117 5 1 1 85 1 117 2.23e-48 153
MsG0280009356.01.T01 MTR_8g464460 62.393 117 12 1 1 85 1 117 1.94e-43 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009356.01.T02 AT2G05840 58.974 117 16 1 1 85 1 117 2.52e-42 137
MsG0280009356.01.T02 AT2G05840 58.974 117 16 1 1 85 1 117 3.31e-42 137
MsG0280009356.01.T02 AT2G05840 58.974 117 16 1 1 85 1 117 4.58e-42 137
MsG0280009356.01.T02 AT5G35590 58.974 117 16 1 1 85 1 117 8.77e-42 137
MsG0280009356.01.T01 AT2G05840 58.974 117 16 1 1 85 1 117 2.52e-42 137
MsG0280009356.01.T01 AT2G05840 58.974 117 16 1 1 85 1 117 3.31e-42 137
MsG0280009356.01.T01 AT2G05840 58.974 117 16 1 1 85 1 117 4.58e-42 137
MsG0280009356.01.T01 AT5G35590 58.974 117 16 1 1 85 1 117 8.77e-42 137

Find 16 sgRNAs with CRISPR-Local

Find 307 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTACTTTCCCTTGGATA+AGG 0.374756 2:+50748800 None:intergenic
CTGCCAAAGAAAAGGAGAAT+AGG 0.379463 2:+50748875 None:intergenic
TGCCAAAGAAAAGGAGAATA+GGG 0.429228 2:+50748876 None:intergenic
GTTTATATATTACTTTCCCT+TGG 0.447491 2:+50748794 None:intergenic
ACTTGAAAGAGACGCCCCTC+GGG 0.470636 2:+50748721 None:intergenic
CGTCATTCTTTCCTTATCCA+AGG 0.474461 2:-50748811 MsG0280009356.01.T02:five_prime_UTR
TCAGATTCCTGCCAAAGAAA+AGG 0.501151 2:+50748867 None:intergenic
GTCATTCTTTCCTTATCCAA+GGG 0.527964 2:-50748810 MsG0280009356.01.T02:five_prime_UTR
ATAAACATGAGTCGAGGAAG+TGG 0.531301 2:-50748777 MsG0280009356.01.T02:exon
ATGAGTCGAGGAAGTGGAGG+TGG 0.536279 2:-50748771 MsG0280009356.01.T02:CDS
CACTTGAAAGAGACGCCCCT+CGG 0.542486 2:+50748720 None:intergenic
CGAGGGGCGTCTCTTTCAAG+TGG 0.558759 2:-50748719 MsG0280009356.01.T02:CDS
AACATGAGTCGAGGAAGTGG+AGG 0.632274 2:-50748774 MsG0280009356.01.T02:CDS
GAGGGGCGTCTCTTTCAAGT+GGG 0.638985 2:-50748718 MsG0280009356.01.T02:intron
TAATATATAAACATGAGTCG+AGG 0.668937 2:-50748783 MsG0280009356.01.T02:exon
CTTGAAAGAGACGCCCCTCG+GGG 0.677610 2:+50748722 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCAAAATTAAAACAAAAT+GGG + Chr2:50747660-50747679 None:intergenic 10.0%
!! TAATTTATTAATCATACTTT+GGG + Chr2:50748684-50748703 None:intergenic 10.0%
!! TTAATTTATTAATCATACTT+TGG + Chr2:50748685-50748704 None:intergenic 10.0%
!!! TAATATTGAATTTTGAAAAA+TGG + Chr2:50746441-50746460 None:intergenic 10.0%
!! AAATTCTTTAATAGTAAACA+TGG - Chr2:50749235-50749254 MsG0280009356.01.T02:intron 15.0%
!! CATCTAGTAAAATATAAATT+AGG - Chr2:50748512-50748531 MsG0280009356.01.T02:intron 15.0%
!! CATTCAAAATTAAAACAAAA+TGG + Chr2:50747661-50747680 None:intergenic 15.0%
!! GTAATTACACATATTATTTA+GGG + Chr2:50749382-50749401 None:intergenic 15.0%
!! TAATTACACATATTATTTAG+GGG + Chr2:50749381-50749400 None:intergenic 15.0%
!! TACTCTAATAGAATAAATAA+TGG + Chr2:50750135-50750154 None:intergenic 15.0%
!!! AAACAATTTAAATTCTAGAA+AGG + Chr2:50745663-50745682 None:intergenic 15.0%
!!! CATATTTAAATACTTTTCAT+TGG + Chr2:50747240-50747259 None:intergenic 15.0%
!!! TTTAATAAAAGTACCTATTA+AGG - Chr2:50747177-50747196 MsG0280009356.01.T02:intron 15.0%
!!! TTTATTACTTTTTTAAACGT+GGG - Chr2:50747419-50747438 MsG0280009356.01.T02:intron 15.0%
!!! TTTGTTTTAATTTTGAATGT+GGG - Chr2:50747662-50747681 MsG0280009356.01.T02:intron 15.0%
!!! TTTTAATCATTCATACAAAT+AGG + Chr2:50749334-50749353 None:intergenic 15.0%
!!! TTTTATTACTTTTTTAAACG+TGG - Chr2:50747418-50747437 MsG0280009356.01.T02:intron 15.0%
!!! TTTTGTTTTAATTTTGAATG+TGG - Chr2:50747661-50747680 MsG0280009356.01.T02:intron 15.0%
!! ATATATCATAGTTCAACTTA+TGG + Chr2:50748831-50748850 None:intergenic 20.0%
!! ATCCCAAATATAAAATAGAT+GGG + Chr2:50748633-50748652 None:intergenic 20.0%
!! GTTTAATATGTAAAGATAGT+GGG + Chr2:50747264-50747283 None:intergenic 20.0%
!! TAAGTATAGACTATAGATAA+TGG - Chr2:50748792-50748811 MsG0280009356.01.T02:exon 20.0%
!! TATTACTTCTTTATACAAGT+GGG - Chr2:50747380-50747399 MsG0280009356.01.T02:intron 20.0%
!! TGTTTAATATGTAAAGATAG+TGG + Chr2:50747265-50747284 None:intergenic 20.0%
!! TTATTACTTCTTTATACAAG+TGG - Chr2:50747379-50747398 MsG0280009356.01.T02:intron 20.0%
!!! ATGTTTTGTTTGTTCTATTT+AGG - Chr2:50745806-50745825 MsG0280009356.01.T02:three_prime_UTR 20.0%
!!! CATATTTTTTGTTTGTGTAA+GGG - Chr2:50746557-50746576 MsG0280009356.01.T02:three_prime_UTR 20.0%
!!! CTCTTTTATAAAAAATAGTC+AGG + Chr2:50746174-50746193 None:intergenic 20.0%
!!! GACTATCTAAATTGATTTTA+AGG - Chr2:50747755-50747774 MsG0280009356.01.T02:intron 20.0%
!!! GTTAACATCAATCTAAAATT+GGG - Chr2:50747919-50747938 MsG0280009356.01.T02:intron 20.0%
!!! TATTACTCATTTTTACAAGT+GGG - Chr2:50747298-50747317 MsG0280009356.01.T02:intron 20.0%
!!! TCATATTTTTTGTTTGTGTA+AGG - Chr2:50746556-50746575 MsG0280009356.01.T02:three_prime_UTR 20.0%
!!! TGTTAACATCAATCTAAAAT+TGG - Chr2:50747918-50747937 MsG0280009356.01.T02:intron 20.0%
! AAACCTGATATTATCTGAAA+CGG - Chr2:50748542-50748561 MsG0280009356.01.T02:intron 25.0%
! AAATAAGCTGAAACAACTTA+TGG - Chr2:50746270-50746289 MsG0280009356.01.T02:three_prime_UTR 25.0%
! AAATAAGTTGTGAATAGCAT+AGG + Chr2:50746100-50746119 None:intergenic 25.0%
! AAATCATAATAGGCTAACAA+CGG + Chr2:50749114-50749133 None:intergenic 25.0%
! AATAAAAGAATTACATGCAG+TGG + Chr2:50749453-50749472 None:intergenic 25.0%
! AATTAGTTGTTTATCCAATC+AGG - Chr2:50746147-50746166 MsG0280009356.01.T02:three_prime_UTR 25.0%
! CATCCCAAATATAAAATAGA+TGG + Chr2:50748634-50748653 None:intergenic 25.0%
! CATTATTATAACACATCTGT+TGG - Chr2:50749508-50749527 MsG0280009356.01.T02:intron 25.0%
! CGCAAAAATAATAGAGAAAA+AGG + Chr2:50750268-50750287 None:intergenic 25.0%
! GATGTTTCCTAAATCATAAT+AGG + Chr2:50749124-50749143 None:intergenic 25.0%
! GTAATAAAATGTGTACTACT+TGG + Chr2:50747325-50747344 None:intergenic 25.0%
! GTTCAAAAGTTATCACAAAA+TGG + Chr2:50750205-50750224 None:intergenic 25.0%
! GTTTATATATTACTTTCCCT+TGG + Chr2:50746994-50747013 None:intergenic 25.0%
! TAATATATAAACATGAGTCG+AGG - Chr2:50747002-50747021 MsG0280009356.01.T02:intron 25.0%
! TATTACTTCTTTATGCAAGT+GGG - Chr2:50747339-50747358 MsG0280009356.01.T02:intron 25.0%
! TCTGTAATCATATATCTAGT+TGG + Chr2:50748771-50748790 None:intergenic 25.0%
! TTATTACTTCTTTATGCAAG+TGG - Chr2:50747338-50747357 MsG0280009356.01.T02:intron 25.0%
!! AACCATTTTATAACAGCTTT+TGG + Chr2:50750318-50750337 None:intergenic 25.0%
!! ACCATTTTATAACAGCTTTT+GGG + Chr2:50750317-50750336 None:intergenic 25.0%
!! ATTGATTTATTCACACTTTC+TGG - Chr2:50747871-50747890 MsG0280009356.01.T02:intron 25.0%
!! CACCCATCTATTTTATATTT+GGG - Chr2:50748628-50748647 MsG0280009356.01.T02:intron 25.0%
!! TAAGTTGAAATATTCATTGC+AGG - Chr2:50748265-50748284 MsG0280009356.01.T02:intron 25.0%
!! TTGTTAGCCTATTATGATTT+AGG - Chr2:50749114-50749133 MsG0280009356.01.T02:intron 25.0%
!! TTTTACTAGATGCAATAAAG+TGG + Chr2:50748504-50748523 None:intergenic 25.0%
!!! CATGGCAATTTAATTTTATG+TGG - Chr2:50749253-50749272 MsG0280009356.01.T02:intron 25.0%
!!! CTATTACTCATTTTTACAAG+TGG - Chr2:50747297-50747316 MsG0280009356.01.T02:intron 25.0%
!!! CTTATTTTTAGGAGAACTTA+TGG - Chr2:50746113-50746132 MsG0280009356.01.T02:three_prime_UTR 25.0%
!!! GAATTTTTTCATTACTGATC+AGG + Chr2:50749221-50749240 None:intergenic 25.0%
!!! GCTATTCACAACTTATTTTT+AGG - Chr2:50746102-50746121 MsG0280009356.01.T02:three_prime_UTR 25.0%
!!! TCTATTATTTTTGCGCAATA+AGG - Chr2:50750273-50750292 MsG0280009356.01.T02:intron 25.0%
!!! TTACAATGTGGTGTTTTAAT+TGG - Chr2:50747598-50747617 MsG0280009356.01.T02:intron 25.0%
!!! TTAGACTTTATTTTGCTTGT+AGG - Chr2:50748223-50748242 MsG0280009356.01.T02:intron 25.0%
!!! TTTTTTTCTTCTCTAAGTGT+TGG + Chr2:50746946-50746965 None:intergenic 25.0%
AAATGTTCTGATTTCTGACA+GGG - Chr2:50749193-50749212 MsG0280009356.01.T02:intron 30.0%
AAGAAGAAGAATAGTCTTTG+GGG + Chr2:50746384-50746403 None:intergenic 30.0%
ACAAAATCTCTTGACACAAT+TGG + Chr2:50746473-50746492 None:intergenic 30.0%
ACATTTGAGTAAGAAATGGT+AGG + Chr2:50749179-50749198 None:intergenic 30.0%
AGAAGAAGAAGAATAGTCTT+TGG + Chr2:50746386-50746405 None:intergenic 30.0%
ATAAGCTATCTTGGAGAATT+AGG + Chr2:50746019-50746038 None:intergenic 30.0%
ATATTACTTTCCCTTGGATA+AGG + Chr2:50746988-50747007 None:intergenic 30.0%
ATCTGAGTAAAAGAAGATCA+CGG - Chr2:50745616-50745635 MsG0280009356.01.T02:three_prime_UTR 30.0%
ATTTACACAGCATTGTAATG+AGG - Chr2:50747975-50747994 MsG0280009356.01.T02:intron 30.0%
CAGAACATTTGAGTAAGAAA+TGG + Chr2:50749183-50749202 None:intergenic 30.0%
CTGCTTGTATAAACTTTAAC+AGG + Chr2:50748462-50748481 None:intergenic 30.0%
GAACAAATATCTTATCGTGT+TGG - Chr2:50748905-50748924 MsG0280009356.01.T02:intron 30.0%
GAAGAAGAAGAATAGTCTTT+GGG + Chr2:50746385-50746404 None:intergenic 30.0%
GCATCTCATATCCAAATTTA+TGG + Chr2:50748998-50749017 None:intergenic 30.0%
GCTGATAAAATTCGTATCAT+GGG - Chr2:50746687-50746706 MsG0280009356.01.T02:three_prime_UTR 30.0%
GTGTAAAAGAATACACAAGT+CGG + Chr2:50749601-50749620 None:intergenic 30.0%
GTTATGCAACAAACAAGTAT+GGG + Chr2:50745786-50745805 None:intergenic 30.0%
GTTCTATTTAGGAAACATCA+TGG - Chr2:50745817-50745836 MsG0280009356.01.T02:three_prime_UTR 30.0%
TAAGCTATCTTGGAGAATTA+GGG + Chr2:50746018-50746037 None:intergenic 30.0%
TACAATGCTGTGTAAATCTT+TGG + Chr2:50747972-50747991 None:intergenic 30.0%
TCCCAAAAGCTGTTATAAAA+TGG - Chr2:50750313-50750332 MsG0280009356.01.T02:intron 30.0%
TGATGATATAATGTCGTGTA+TGG - Chr2:50749724-50749743 MsG0280009356.01.T02:intron 30.0%
TGTTATGCAACAAACAAGTA+TGG + Chr2:50745787-50745806 None:intergenic 30.0%
TTAGGAGAACTTATGGTATA+AGG - Chr2:50746120-50746139 MsG0280009356.01.T02:three_prime_UTR 30.0%
TTTCATTTCATAATCTAGCC+TGG - Chr2:50746236-50746255 MsG0280009356.01.T02:three_prime_UTR 30.0%
TTTGTGATGACAAATTCAAG+TGG + Chr2:50747949-50747968 None:intergenic 30.0%
! AGATTTTGTTAGCATCCTTT+TGG - Chr2:50746484-50746503 MsG0280009356.01.T02:three_prime_UTR 30.0%
! GCACCCATCTATTTTATATT+TGG - Chr2:50748627-50748646 MsG0280009356.01.T02:intron 30.0%
! TAGTGAAACGTTTTACAATG+TGG - Chr2:50747586-50747605 MsG0280009356.01.T02:intron 30.0%
! TATTGCAATCTAAGAGCATA+CGG + Chr2:50749487-50749506 None:intergenic 30.0%
! TCATGTTTCTACCGAAAAAA+AGG - Chr2:50747805-50747824 MsG0280009356.01.T02:intron 30.0%
! TGGTAGAAAAGTTGTCATTT+AGG - Chr2:50746794-50746813 MsG0280009356.01.T02:CDS 30.0%
!! CAATCGATGTTTTGTTCAAT+AGG + Chr2:50746513-50746532 None:intergenic 30.0%
!! TTGAATTGAGTTGACTAGTT+TGG - Chr2:50748050-50748069 MsG0280009356.01.T02:intron 30.0%
!!! TTTTTTCGGTAGAAACATGA+GGG + Chr2:50747805-50747824 None:intergenic 30.0%
!!! TTTTTTTCGGTAGAAACATG+AGG + Chr2:50747806-50747825 None:intergenic 30.0%
AAAAATAGTCAGGACCTGAT+TGG + Chr2:50746164-50746183 None:intergenic 35.0%
AAATAAGTGATGTGTCACGT+GGG + Chr2:50747150-50747169 None:intergenic 35.0%
AAGCCGTTTCAGATAATATC+AGG + Chr2:50748548-50748567 None:intergenic 35.0%
ACTAAAACAAGCATCTCCAA+TGG + Chr2:50747570-50747589 None:intergenic 35.0%
AGAAAATTTACCTACCCTAG+TGG + Chr2:50749676-50749695 None:intergenic 35.0%
ATGCTTATATGAGACCACTA+GGG - Chr2:50749659-50749678 MsG0280009356.01.T02:intron 35.0%
CAAATGTTCTGATTTCTGAC+AGG - Chr2:50749192-50749211 MsG0280009356.01.T02:intron 35.0%
CAAGTAAAACAACACACAAC+AGG + Chr2:50748028-50748047 None:intergenic 35.0%
CACTAAGAACTTAAAGCTTC+TGG + Chr2:50746776-50746795 None:intergenic 35.0%
CAGATCTGCAACAAAAATGA+TGG + Chr2:50748392-50748411 None:intergenic 35.0%
CATATTATTTAGGGGAATGC+TGG + Chr2:50749373-50749392 None:intergenic 35.0%
CCAATGGCAATACACTAATA+AGG - Chr2:50747211-50747230 MsG0280009356.01.T02:intron 35.0%
CCTTATTAGTGTATTGCCAT+TGG + Chr2:50747214-50747233 None:intergenic 35.0%
CTAAAACAAGCATCTCCAAT+GGG + Chr2:50747569-50747588 None:intergenic 35.0%
CTATGCTATTAGCAAGTCAT+TGG - Chr2:50749148-50749167 MsG0280009356.01.T02:intron 35.0%
CTATTAAGGAGTCCTTGTTA+GGG + Chr2:50747125-50747144 None:intergenic 35.0%
GCAATAAGGAGAAACAAAGA+AGG - Chr2:50750287-50750306 MsG0280009356.01.T02:intron 35.0%
GCCTGTATACTTTCTATGAT+CGG + Chr2:50746669-50746688 None:intergenic 35.0%
GGCTGATAAAATTCGTATCA+TGG - Chr2:50746686-50746705 MsG0280009356.01.T02:three_prime_UTR 35.0%
GTCATTCTTTCCTTATCCAA+GGG - Chr2:50746975-50746994 MsG0280009356.01.T02:intron 35.0%
GTTAACAGAGTATGCTTTCA+AGG - Chr2:50748100-50748119 MsG0280009356.01.T02:intron 35.0%
TAAAACAAGCATCTCCAATG+GGG + Chr2:50747568-50747587 None:intergenic 35.0%
TAAATAAGTGATGTGTCACG+TGG + Chr2:50747151-50747170 None:intergenic 35.0%
TAAATTGTGAGCATCCACAA+TGG - Chr2:50747094-50747113 MsG0280009356.01.T02:intron 35.0%
TAGAAAACAATTGCCAACCT+AGG + Chr2:50747839-50747858 None:intergenic 35.0%
TAGATCTATGATGTCAATCG+CGG + Chr2:50745577-50745596 None:intergenic 35.0%
TCATATATCTAGTTGGCCAT+AGG + Chr2:50748764-50748783 None:intergenic 35.0%
TCTATTAAGGAGTCCTTGTT+AGG + Chr2:50747126-50747145 None:intergenic 35.0%
TGCCAAAGAAAAGGAGAATA+GGG + Chr2:50746912-50746931 None:intergenic 35.0%
TGCTTTAATTAGGCTGTGTT+TGG - Chr2:50745845-50745864 MsG0280009356.01.T02:three_prime_UTR 35.0%
TTGTCTCAATGCATAGTCAT+TGG - Chr2:50746723-50746742 MsG0280009356.01.T02:three_prime_UTR 35.0%
TTGTTCAATAGGAAGCCAAA+AGG + Chr2:50746502-50746521 None:intergenic 35.0%
! AAACCAAGGTACTTAGTGAT+GGG + Chr2:50748329-50748348 None:intergenic 35.0%
! AATTTTTCTTCCTCCAATGG+AGG - Chr2:50747505-50747524 MsG0280009356.01.T02:intron 35.0%
! ACCGATCATAGAAAGTATAC+AGG - Chr2:50746665-50746684 MsG0280009356.01.T02:three_prime_UTR 35.0%
! ATTGCAATCTAAGAGCATAC+GGG + Chr2:50749486-50749505 None:intergenic 35.0%
! ATTTTTCTTCCTCCAATGGA+GGG - Chr2:50747506-50747525 MsG0280009356.01.T02:intron 35.0%
! CTCAATTTTTCTTCCTCCAA+TGG - Chr2:50747502-50747521 MsG0280009356.01.T02:intron 35.0%
! GGTTCAGATCTTTTATGTAC+TGG - Chr2:50747629-50747648 MsG0280009356.01.T02:intron 35.0%
! GTGTATTCTTTTACACGAAC+AGG - Chr2:50749605-50749624 MsG0280009356.01.T02:intron 35.0%
! TAAACCAAGGTACTTAGTGA+TGG + Chr2:50748330-50748349 None:intergenic 35.0%
! TACCCTATTCTCCTTTTCTT+TGG - Chr2:50746907-50746926 MsG0280009356.01.T02:intron 35.0%
! TTAGCTTATCGAGACTGTTT+TGG - Chr2:50745927-50745946 MsG0280009356.01.T02:three_prime_UTR 35.0%
!! AATTTTATGTGGTGTCGAGT+AGG - Chr2:50749264-50749283 MsG0280009356.01.T02:intron 35.0%
!! ATTGAGTTGACTAGTTTGGT+AGG - Chr2:50748054-50748073 MsG0280009356.01.T02:intron 35.0%
!! ATTTTATGTGGTGTCGAGTA+GGG - Chr2:50749265-50749284 MsG0280009356.01.T02:intron 35.0%
!! CAATGACTTGCTAATAGCAT+AGG + Chr2:50749150-50749169 None:intergenic 35.0%
!! CAGAAGCTTTAAGTTCTTAG+TGG - Chr2:50746774-50746793 MsG0280009356.01.T02:CDS 35.0%
!! CATGGTCTTGTGCTTTAATT+AGG - Chr2:50745835-50745854 MsG0280009356.01.T02:three_prime_UTR 35.0%
!! CTGAAACGGCTTGTTAATTA+GGG - Chr2:50748556-50748575 MsG0280009356.01.T02:intron 35.0%
!! GCTGTTTTCATAAGCTATCT+TGG + Chr2:50746028-50746047 None:intergenic 35.0%
!! TCTGAAACGGCTTGTTAATT+AGG - Chr2:50748555-50748574 MsG0280009356.01.T02:intron 35.0%
!! TGAAACGGCTTGTTAATTAG+GGG - Chr2:50748557-50748576 MsG0280009356.01.T02:intron 35.0%
!! TGGGAATTGATGACGAAAAT+GGG - Chr2:50749839-50749858 MsG0280009356.01.T02:intron 35.0%
!! TTGGGAATTGATGACGAAAA+TGG - Chr2:50749838-50749857 MsG0280009356.01.T02:intron 35.0%
!!! GTGGTGTTTTAATTGGTCAA+AGG - Chr2:50747605-50747624 MsG0280009356.01.T02:intron 35.0%
!!! GTGTTTTAATTGGTCAAAGG+TGG - Chr2:50747608-50747627 MsG0280009356.01.T02:intron 35.0%
!!! TTTTTTGTTTGTGTAAGGGC+TGG - Chr2:50746561-50746580 MsG0280009356.01.T02:three_prime_UTR 35.0%
AAAAAAAGGACCGTTGACCT+AGG - Chr2:50747819-50747838 MsG0280009356.01.T02:intron 40.0%
AAAACAAGCATCTCCAATGG+GGG + Chr2:50747567-50747586 None:intergenic 40.0%
AAGGAACGGGCAACTATATA+AGG + Chr2:50747474-50747493 None:intergenic 40.0%
AATAAGTGATGTGTCACGTG+GGG + Chr2:50747149-50747168 None:intergenic 40.0%
ACAATGGTAGTTCCCTAACA+AGG - Chr2:50747110-50747129 MsG0280009356.01.T02:intron 40.0%
ACATTCCAAATCTAGGAGCA+AGG + Chr2:50746605-50746624 None:intergenic 40.0%
ACCATCAAAGTGACTTTCCT+TGG - Chr2:50746747-50746766 MsG0280009356.01.T02:intron 40.0%
ACTTTGGTCACAAGGTACAT+AGG - Chr2:50749896-50749915 MsG0280009356.01.T02:intron 40.0%
ATAAACATGAGTCGAGGAAG+TGG - Chr2:50747008-50747027 MsG0280009356.01.T02:intron 40.0%
ATTGTGCTGCAAAGTTGCTA+TGG - Chr2:50749813-50749832 MsG0280009356.01.T02:intron 40.0%
ATTTGGATATGAGATGCCTG+TGG - Chr2:50749001-50749020 MsG0280009356.01.T02:intron 40.0%
CACTAAGTACCTTGGTTTAC+TGG - Chr2:50748331-50748350 MsG0280009356.01.T02:intron 40.0%
CATGCTTATATGAGACCACT+AGG - Chr2:50749658-50749677 MsG0280009356.01.T02:intron 40.0%
CATTCATGGAGCTCAATTGT+AGG + Chr2:50749414-50749433 None:intergenic 40.0%
CATTCCAAATCTAGGAGCAA+GGG + Chr2:50746604-50746623 None:intergenic 40.0%
CCGATCAGAAAAAGAGAGAA+GGG + Chr2:50745484-50745503 None:intergenic 40.0%
CGTCAAAGATTGCAGTGAAA+AGG + Chr2:50749702-50749721 None:intergenic 40.0%
CGTCATTCTTTCCTTATCCA+AGG - Chr2:50746974-50746993 MsG0280009356.01.T02:intron 40.0%
CTGCCAAAGAAAAGGAGAAT+AGG + Chr2:50746913-50746932 None:intergenic 40.0%
CTTAAAGCTTCTGGATACCA+AGG + Chr2:50746767-50746786 None:intergenic 40.0%
CTTATTTCCATAAGCTCTCC+AGG + Chr2:50746257-50746276 None:intergenic 40.0%
CTTTCCCACATTCCAAATCT+AGG + Chr2:50746612-50746631 None:intergenic 40.0%
GAAAAATTGAGTCCCCGTTA+AGG + Chr2:50747493-50747512 None:intergenic 40.0%
GCTGAGTTTCGCCATAAATT+TGG - Chr2:50748984-50749003 MsG0280009356.01.T02:intron 40.0%
GGAGTCATTGAGTCCTTAAT+AGG + Chr2:50747193-50747212 None:intergenic 40.0%
GGGTCACATTTGTAAAGTTG+AGG + Chr2:50749865-50749884 None:intergenic 40.0%
GTATCATGGGAACTGTTTGT+TGG - Chr2:50746700-50746719 MsG0280009356.01.T02:three_prime_UTR 40.0%
GTATGCTTTCAAGGCTGTTA+AGG - Chr2:50748109-50748128 MsG0280009356.01.T02:intron 40.0%
TAAGGACTCAATGACTCCAA+TGG - Chr2:50747195-50747214 MsG0280009356.01.T02:intron 40.0%
TATAGTTGCCCGTTCCTTAA+CGG - Chr2:50747476-50747495 MsG0280009356.01.T02:CDS 40.0%
TCAGATTCCTGCCAAAGAAA+AGG + Chr2:50746921-50746940 None:intergenic 40.0%
TCCGATCAGAAAAAGAGAGA+AGG + Chr2:50745485-50745504 None:intergenic 40.0%
TCGTTACTCAGAAAAGGTTC+CGG - Chr2:50748180-50748199 MsG0280009356.01.T02:intron 40.0%
TCTCTCTCCTGAGTAACTAA+CGG + Chr2:50747447-50747466 None:intergenic 40.0%
TGCAAAGTTGCTATGGTCTT+GGG - Chr2:50749820-50749839 MsG0280009356.01.T02:intron 40.0%
TTAGTTACTCAGGAGAGAGA+GGG - Chr2:50747447-50747466 MsG0280009356.01.T02:CDS 40.0%
TTATATGAGACCACTAGGGT+AGG - Chr2:50749663-50749682 MsG0280009356.01.T02:intron 40.0%
TTGTTAGGGAACTACCATTG+TGG + Chr2:50747111-50747130 None:intergenic 40.0%
TTTCCCATCACTAAGTACCT+TGG - Chr2:50748323-50748342 MsG0280009356.01.T02:intron 40.0%
TTTGCGTCGTTACTCAGAAA+AGG - Chr2:50748174-50748193 MsG0280009356.01.T02:intron 40.0%
! ACATTACTATTTTCTCCCCG+AGG - Chr2:50747048-50747067 MsG0280009356.01.T02:intron 40.0%
! ACCAAGGAAAGTCACTTTGA+TGG + Chr2:50746751-50746770 None:intergenic 40.0%
! ATTACTATTTTCTCCCCGAG+GGG - Chr2:50747050-50747069 MsG0280009356.01.T02:intron 40.0%
! CATTACTATTTTCTCCCCGA+GGG - Chr2:50747049-50747068 MsG0280009356.01.T02:intron 40.0%
! CCCTTCTCTCTTTTTCTGAT+CGG - Chr2:50745481-50745500 MsG0280009356.01.T02:three_prime_UTR 40.0%
! CTATTCTCCTTTTCTTTGGC+AGG - Chr2:50746911-50746930 MsG0280009356.01.T02:intron 40.0%
! TGCTCCTAGATTTGGAATGT+GGG - Chr2:50746605-50746624 MsG0280009356.01.T02:three_prime_UTR 40.0%
! TTGCAATCTAAGAGCATACG+GGG + Chr2:50749485-50749504 None:intergenic 40.0%
! TTGCTCCTAGATTTGGAATG+TGG - Chr2:50746604-50746623 MsG0280009356.01.T02:three_prime_UTR 40.0%
!! ACTTTCTGGTTTGCATTGCA+CGG - Chr2:50747885-50747904 MsG0280009356.01.T02:intron 40.0%
!! ATACTTTGGGTTGAGTTGCA+TGG + Chr2:50748671-50748690 None:intergenic 40.0%
!! GAAACGGCTTGTTAATTAGG+GGG - Chr2:50748558-50748577 MsG0280009356.01.T02:intron 40.0%
!! TACTTTGGGTTGAGTTGCAT+GGG + Chr2:50748670-50748689 None:intergenic 40.0%
!! TTGAGTTGCATGGGATTTTG+TGG + Chr2:50748661-50748680 None:intergenic 40.0%
!! TTTTATGTGGTGTCGAGTAG+GGG - Chr2:50749266-50749285 MsG0280009356.01.T02:intron 40.0%
!!! AGGTCAACGGTCCTTTTTTT+CGG + Chr2:50747819-50747838 None:intergenic 40.0%
AAACAAGCATCTCCAATGGG+GGG + Chr2:50747566-50747585 None:intergenic 45.0%
AAATCTAGGAGCAAGGGAAC+GGG + Chr2:50746598-50746617 None:intergenic 45.0%
AATCTAGCCTGGAGAGCTTA+TGG - Chr2:50746247-50746266 MsG0280009356.01.T02:three_prime_UTR 45.0%
ACCAATTGAGGTTATCCCTG+CGG + Chr2:50748135-50748154 None:intergenic 45.0%
AGAGAGAAGGGAAACAAACC+TGG + Chr2:50745472-50745491 None:intergenic 45.0%
AGCTCCATGAATGTGCTTCT+TGG - Chr2:50749421-50749440 MsG0280009356.01.T02:intron 45.0%
AGCTGTGTCTTTGAACAAGC+TGG - Chr2:50745420-50745439 MsG0280009356.01.T02:three_prime_UTR 45.0%
AGTCAACTTCCTGTACTCAC+CGG + Chr2:50748202-50748221 None:intergenic 45.0%
ATAGTTGCCCGTTCCTTAAC+GGG - Chr2:50747477-50747496 MsG0280009356.01.T02:CDS 45.0%
ATAGTTGTGCATGCTGCCTA+TGG - Chr2:50748745-50748764 MsG0280009356.01.T02:CDS 45.0%
ATTGAGTCCCCGTTAAGGAA+CGG + Chr2:50747488-50747507 None:intergenic 45.0%
CAAATCTAGGAGCAAGGGAA+CGG + Chr2:50746599-50746618 None:intergenic 45.0%
CAATTGCCAACCTAGGTCAA+CGG + Chr2:50747832-50747851 None:intergenic 45.0%
CATTTAGCCAACACATCCAC+AGG + Chr2:50749020-50749039 None:intergenic 45.0%
CCTAGCATCAGCTGAAAAGA+AGG + Chr2:50748934-50748953 None:intergenic 45.0%
CGCAAACAGAATCGTTTCCT+CGG + Chr2:50748160-50748179 None:intergenic 45.0%
CTCTCTCCTGAGTAACTAAC+GGG + Chr2:50747446-50747465 None:intergenic 45.0%
CTGATCGGAATTTCCCGATA+CGG - Chr2:50745496-50745515 MsG0280009356.01.T02:three_prime_UTR 45.0%
CTGCAAAGTTGCTATGGTCT+TGG - Chr2:50749819-50749838 MsG0280009356.01.T02:intron 45.0%
CTTGTGACCAAAGTAATGCC+CGG + Chr2:50749890-50749909 None:intergenic 45.0%
CTTTACAAATGTGACCCTGC+CGG - Chr2:50749868-50749887 MsG0280009356.01.T02:intron 45.0%
GAAAGTCGTCATCATCATCC+AGG - Chr2:50745451-50745470 MsG0280009356.01.T02:three_prime_UTR 45.0%
GCTTCAGTCATTCACATCGA+TGG + Chr2:50750091-50750110 None:intergenic 45.0%
GGAGTGGTCCTTGTTAAGTA+AGG - Chr2:50747539-50747558 MsG0280009356.01.T02:intron 45.0%
GGTAGCTGCTATTGCTGATA+AGG + Chr2:50746648-50746667 None:intergenic 45.0%
GTTAGTTACTCAGGAGAGAG+AGG - Chr2:50747446-50747465 MsG0280009356.01.T02:CDS 45.0%
TACCTTGGTTTACTGGCAAC+TGG - Chr2:50748338-50748357 MsG0280009356.01.T02:intron 45.0%
TTCATTGCAGGACAAGCTCT+TGG - Chr2:50748277-50748296 MsG0280009356.01.T02:intron 45.0%
TTTACAAATGTGACCCTGCC+GGG - Chr2:50749869-50749888 MsG0280009356.01.T02:intron 45.0%
! ATAACCTCAATTGGTGTCCG+AGG - Chr2:50748140-50748159 MsG0280009356.01.T02:intron 45.0%
! CCTTCTTTTCAGCTGATGCT+AGG - Chr2:50748931-50748950 MsG0280009356.01.T02:intron 45.0%
! GTAGCCAAGAAGCACATTCA+TGG + Chr2:50749428-50749447 None:intergenic 45.0%
! TAGCTAGTTTAACCGCTGCA+CGG - Chr2:50745722-50745741 MsG0280009356.01.T02:three_prime_UTR 45.0%
!! CTGTGGATGTGTTGGCTAAA+TGG - Chr2:50749018-50749037 MsG0280009356.01.T02:intron 45.0%
!! TAGGGGAATGCTGGTTTCAA+GGG + Chr2:50749364-50749383 None:intergenic 45.0%
!! TTAGGGGAATGCTGGTTTCA+AGG + Chr2:50749365-50749384 None:intergenic 45.0%
!!! TGTGTAAGGGCTGGCTTTTT+GGG - Chr2:50746570-50746589 MsG0280009356.01.T02:three_prime_UTR 45.0%
!!! TTGTGTAAGGGCTGGCTTTT+TGG - Chr2:50746569-50746588 MsG0280009356.01.T02:three_prime_UTR 45.0%
AAAGGACCGTTGACCTAGGT+TGG - Chr2:50747823-50747842 MsG0280009356.01.T02:intron 50.0%
AACATGAGTCGAGGAAGTGG+AGG - Chr2:50747011-50747030 MsG0280009356.01.T02:intron 50.0%
ATAGGCTAACAACGGCTGTC+TGG + Chr2:50749106-50749125 None:intergenic 50.0%
ATCGGAATTTCCCGATACGG+TGG - Chr2:50745499-50745518 MsG0280009356.01.T02:three_prime_UTR 50.0%
CCTTTCTATTTCCCGTGCAG+CGG + Chr2:50745737-50745756 None:intergenic 50.0%
CGGGCATTACTTTGGTCACA+AGG - Chr2:50749888-50749907 MsG0280009356.01.T02:intron 50.0%
CGTTCCCTTGCTCCTAGATT+TGG - Chr2:50746597-50746616 MsG0280009356.01.T02:three_prime_UTR 50.0%
CTCCAATGGAGGGTCCTTAA+CGG - Chr2:50747516-50747535 MsG0280009356.01.T02:intron 50.0%
GAAAAGGTTCCGGTGAGTAC+AGG - Chr2:50748190-50748209 MsG0280009356.01.T02:intron 50.0%
GGTTTGCATTGCACGGAAGT+TGG - Chr2:50747892-50747911 MsG0280009356.01.T02:intron 50.0%
GTCAATCGCGGTGCTTTGTA+GGG + Chr2:50745565-50745584 None:intergenic 50.0%
GTGCAGCGGTTAAACTAGCT+AGG + Chr2:50745723-50745742 None:intergenic 50.0%
GTGTCACGTGGGGTCTATTA+AGG + Chr2:50747139-50747158 None:intergenic 50.0%
GTTAAGTAAGGACCCCCCAT+TGG - Chr2:50747551-50747570 MsG0280009356.01.T02:intron 50.0%
GTTTCCTCGGACACCAATTG+AGG + Chr2:50748147-50748166 None:intergenic 50.0%
TAGTTGCCCGTTCCTTAACG+GGG - Chr2:50747478-50747497 MsG0280009356.01.T02:CDS 50.0%
TCAATCGCGGTGCTTTGTAG+GGG + Chr2:50745564-50745583 None:intergenic 50.0%
TCCAATGGAGGGTCCTTAAC+GGG - Chr2:50747517-50747536 MsG0280009356.01.T02:intron 50.0%
TGAGATGCCTGTGGATGTGT+TGG - Chr2:50749010-50749029 MsG0280009356.01.T02:intron 50.0%
TGCCAGTTGCCAGTAAACCA+AGG + Chr2:50748343-50748362 None:intergenic 50.0%
TGGGGGGTCCTTACTTAACA+AGG + Chr2:50747550-50747569 None:intergenic 50.0%
TGTCAATCGCGGTGCTTTGT+AGG + Chr2:50745566-50745585 None:intergenic 50.0%
TTACTGGCAACTGGCATGAC+AGG - Chr2:50748347-50748366 MsG0280009356.01.T02:intron 50.0%
TTCAGCTGATGCTAGGACAC+TGG - Chr2:50748938-50748957 MsG0280009356.01.T02:intron 50.0%
TTGAGTCCCCGTTAAGGAAC+GGG + Chr2:50747487-50747506 None:intergenic 50.0%
! AGCTAGTTTAACCGCTGCAC+GGG - Chr2:50745723-50745742 MsG0280009356.01.T02:three_prime_UTR 50.0%
ACAAGGACCACTCCCCGTTA+AGG + Chr2:50747533-50747552 None:intergenic 55.0%
ACTTGAAAGAGACGCCCCTC+GGG + Chr2:50747067-50747086 None:intergenic 55.0%
ATGAGTCGAGGAAGTGGAGG+TGG - Chr2:50747014-50747033 MsG0280009356.01.T02:intron 55.0%
CACTTGAAAGAGACGCCCCT+CGG + Chr2:50747068-50747087 None:intergenic 55.0%
CCAATGGAGGGTCCTTAACG+GGG - Chr2:50747518-50747537 MsG0280009356.01.T02:intron 55.0%
CCGCTGCACGGGAAATAGAA+AGG - Chr2:50745734-50745753 MsG0280009356.01.T02:three_prime_UTR 55.0%
CGAGGATGCAATTCCATCGC+TGG - Chr2:50745526-50745545 MsG0280009356.01.T02:three_prime_UTR 55.0%
CGTTAAGGACCCTCCATTGG+AGG + Chr2:50747518-50747537 None:intergenic 55.0%
GACCAAAGTAATGCCCGGCA+GGG + Chr2:50749885-50749904 None:intergenic 55.0%
GCCGCAGGGATAACCTCAAT+TGG - Chr2:50748131-50748150 MsG0280009356.01.T02:intron 55.0%
GCTAACAACGGCTGTCTGGA+AGG + Chr2:50749102-50749121 None:intergenic 55.0%
GTGCTTTGTAGGGGCGGATA+CGG + Chr2:50745555-50745574 None:intergenic 55.0%
GTGGGACCCGTTAGTTACTC+AGG - Chr2:50747437-50747456 MsG0280009356.01.T02:intron 55.0%
TCAAGGCTGTTAAGGCCGCA+GGG - Chr2:50748117-50748136 MsG0280009356.01.T02:intron 55.0%
TGACCAAAGTAATGCCCGGC+AGG + Chr2:50749886-50749905 None:intergenic 55.0%
TTCAAGGCTGTTAAGGCCGC+AGG - Chr2:50748116-50748135 MsG0280009356.01.T02:intron 55.0%
!! GAGGGGCGTCTCTTTCAAGT+GGG - Chr2:50747067-50747086 MsG0280009356.01.T02:intron 55.0%
ATCGCGGTGCTTTGTAGGGG+CGG + Chr2:50745561-50745580 None:intergenic 60.0%
CCCCGTTAAGGACCCTCCAT+TGG + Chr2:50747521-50747540 None:intergenic 60.0%
CTTGAAAGAGACGCCCCTCG+GGG + Chr2:50747066-50747085 None:intergenic 60.0%
GACCCTGCCGGGCATTACTT+TGG - Chr2:50749880-50749899 MsG0280009356.01.T02:intron 60.0%
!! CGAGGGGCGTCTCTTTCAAG+TGG - Chr2:50747066-50747085 MsG0280009356.01.T02:intron 60.0%
ATCCTCGCGCGCCACCGTAT+CGG + Chr2:50745513-50745532 None:intergenic 65.0%
GCGGATACGGTAGCCAGCGA+TGG + Chr2:50745542-50745561 None:intergenic 65.0%
GGAGGGTCCTTAACGGGGAG+TGG - Chr2:50747523-50747542 MsG0280009356.01.T02:intron 65.0%
TCCTCGCGCGCCACCGTATC+GGG + Chr2:50745512-50745531 None:intergenic 70.0%
TCCCGATACGGTGGCGCGCG+AGG - Chr2:50745508-50745527 MsG0280009356.01.T02:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 50745402 50750405 50745402 ID=MsG0280009356.01;Name=MsG0280009356.01
Chr2 mRNA 50745402 50750405 50745402 ID=MsG0280009356.01.T01;Parent=MsG0280009356.01;Name=MsG0280009356.01.T01;_AED=0.40;_eAED=0.40;_QI=178|0.66|1|1|0.33|0.5|4|1362|85
Chr2 exon 50748719 50748900 50748719 ID=MsG0280009356.01.T01:exon:8262;Parent=MsG0280009356.01.T01
Chr2 five_prime_UTR 50750335 50750405 50750335 ID=MsG0280009356.01.T01:five_prime_utr;Parent=MsG0280009356.01.T01
Chr2 five_prime_UTR 50748794 50748900 50748794 ID=MsG0280009356.01.T01:five_prime_utr;Parent=MsG0280009356.01.T01
Chr2 CDS 50748719 50748793 50748719 ID=MsG0280009356.01.T01:cds;Parent=MsG0280009356.01.T01
Chr2 CDS 50747439 50747520 50747439 ID=MsG0280009356.01.T01:cds;Parent=MsG0280009356.01.T01
Chr2 CDS 50746764 50746864 50746764 ID=MsG0280009356.01.T01:cds;Parent=MsG0280009356.01.T01
Chr2 three_prime_UTR 50745402 50746763 50745402 ID=MsG0280009356.01.T01:three_prime_utr;Parent=MsG0280009356.01.T01
Chr2 mRNA 50745402 50750405 50745402 ID=MsG0280009356.01.T02;Parent=MsG0280009356.01;Name=MsG0280009356.01.T02;_AED=0.41;_eAED=0.41;_QI=152|0.66|1|1|0.33|0.5|4|1362|85
Chr2 exon 50748719 50748874 50748719 ID=MsG0280009356.01.T02:exon:8265;Parent=MsG0280009356.01.T02
Chr2 five_prime_UTR 50750335 50750405 50750335 ID=MsG0280009356.01.T02:five_prime_utr;Parent=MsG0280009356.01.T02
Chr2 five_prime_UTR 50748794 50748874 50748794 ID=MsG0280009356.01.T02:five_prime_utr;Parent=MsG0280009356.01.T02
Chr2 CDS 50748719 50748793 50748719 ID=MsG0280009356.01.T02:cds;Parent=MsG0280009356.01.T02
Chr2 CDS 50747439 50747520 50747439 ID=MsG0280009356.01.T02:cds;Parent=MsG0280009356.01.T02
Chr2 CDS 50746764 50746864 50746764 ID=MsG0280009356.01.T02:cds;Parent=MsG0280009356.01.T02
Chr2 three_prime_UTR 50745402 50746763 50745402 ID=MsG0280009356.01.T02:three_prime_utr;Parent=MsG0280009356.01.T02
Gene Sequence

>MsG0280009356.01.T02

ATGAGTCGAGGAAGTGGAGGTGGATACGATCGTCACATTACTATTTTCTCCCCGAGGGGCGTCTCTTTCAAGTGGGACAAGCTCTTGGATCAGACAAGTGTAACACATCTCTTTCCCATCACTAAGTACCTTGGTTTACTGGCAACTGGCATGACAGCTGATGCTAGGACACTGGTTCAACAAGCAAGAAATGAAGCAGCTGAGTTTCGCCATAAATTTGGATATGAGATGCCTGTGGATGTGTTGGCTAAATGGTAA

>MsG0280009356.01.T01

ATGAGTCGAGGAAGTGGAGGTGGATACGATCGTCACATTACTATTTTCTCCCCGAGGGGCGTCTCTTTCAAGTGGGACAAGCTCTTGGATCAGACAAGTGTAACACATCTCTTTCCCATCACTAAGTACCTTGGTTTACTGGCAACTGGCATGACAGCTGATGCTAGGACACTGGTTCAACAAGCAAGAAATGAAGCAGCTGAGTTTCGCCATAAATTTGGATATGAGATGCCTGTGGATGTGTTGGCTAAATGGTAA
Protein sequence

>MsG0280009356.01.T02

MSRGSGGGYDRHITIFSPRGVSFKWDKLLDQTSVTHLFPITKYLGLLATGMTADARTLVQQARNEAAEFRHKFGYEMPVDVLAKW*

>MsG0280009356.01.T01

MSRGSGGGYDRHITIFSPRGVSFKWDKLLDQTSVTHLFPITKYLGLLATGMTADARTLVQQARNEAAEFRHKFGYEMPVDVLAKW*