AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006567.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006567.01.T02 MTR_2g010840 96.454 141 4 1 1 141 37 176 1.02e-97 278
MsG0280006567.01.T02 MTR_4g092130 72.868 129 34 1 1 129 37 164 5.67e-68 203
MsG0280006567.01.T02 MTR_2g089050 60.976 123 47 1 1 123 37 158 1.02e-50 159
MsG0280006567.01.T02 MTR_4g045673 57.724 123 51 1 1 123 37 158 8.16e-50 157
MsG0280006567.01.T01 MTR_2g010840 96.610 177 5 1 1 177 1 176 3.21e-125 350
MsG0280006567.01.T01 MTR_4g092130 73.333 165 43 1 1 165 1 164 2.88e-90 261
MsG0280006567.01.T01 MTR_2g089050 60.377 159 62 1 1 159 1 158 1.92e-68 205
MsG0280006567.01.T01 MTR_4g045673 57.862 159 66 1 1 159 1 158 1.78e-66 200
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006567.01.T02 AT4G21310 64.567 127 44 1 1 127 37 162 4.55e-59 181
MsG0280006567.01.T02 AT4G21310 64.800 125 43 1 3 127 1 124 3.73e-58 177
MsG0280006567.01.T02 AT2G32280 52.344 128 60 1 1 128 37 163 1.44e-49 156
MsG0280006567.01.T02 AT2G32280 52.344 128 60 1 1 128 37 163 1.44e-49 156
MsG0280006567.01.T02 AT1G11500 47.727 132 66 2 9 139 24 153 8.62e-38 126
MsG0280006567.01.T02 AT1G11500 47.727 132 66 2 9 139 57 186 1.05e-37 127
MsG0280006567.01.T02 AT1G11500 47.727 132 66 2 9 139 52 181 1.22e-37 127
MsG0280006567.01.T02 AT1G05291 33.871 124 73 4 6 124 50 169 3.40e-11 58.9
MsG0280006567.01.T01 AT4G21310 65.644 163 55 1 1 163 1 162 3.87e-79 233
MsG0280006567.01.T01 AT2G32280 51.829 164 78 1 1 164 1 163 4.22e-64 194
MsG0280006567.01.T01 AT2G32280 51.829 164 78 1 1 164 1 163 4.22e-64 194
MsG0280006567.01.T01 AT4G21310 64.800 125 43 1 39 163 1 124 1.01e-57 177
MsG0280006567.01.T01 AT1G11500 44.324 185 91 5 1 175 1 183 4.40e-46 149
MsG0280006567.01.T01 AT1G11500 44.324 185 91 5 1 175 6 188 4.48e-46 150
MsG0280006567.01.T01 AT1G11500 48.507 134 64 3 45 175 24 155 2.41e-37 126
MsG0280006567.01.T01 AT1G05291 35.714 154 87 5 12 160 23 169 2.53e-17 76.3

Find 18 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGACAAGTATGCAAATA+AGG 0.285775 2:+3522401 None:intergenic
TAAGAGCTGCAAGTCCTAAT+TGG 0.301626 2:+3522056 None:intergenic
AGCCATCAAGCTTTCCAATT+AGG 0.307387 2:-3522070 MsG0280006567.01.T01:CDS
TCAAATATCCACATCTTCAT+AGG 0.374996 2:+3522106 None:intergenic
GATATTGTTGCAGGCATACT+TGG 0.378516 2:-3522373 MsG0280006567.01.T01:CDS
CATATAATTGCTAACTTGCT+TGG 0.379275 2:-3522016 MsG0280006567.01.T01:CDS
TTGGAGGCTGCGTTTGTGTT+TGG 0.397312 2:-3521997 MsG0280006567.01.T01:CDS
GTCCTAATTGGAAAGCTTGA+TGG 0.422083 2:+3522068 None:intergenic
AGCCACAGATAATTGTCTAT+TGG 0.431607 2:+3521946 None:intergenic
CCTTATTTGCATACTTGTCA+TGG 0.434363 2:-3522401 MsG0280006567.01.T01:CDS
AGATGTGGATATTTGATTGT+AGG 0.471306 2:-3522099 MsG0280006567.01.T01:CDS
AATTGGAAAGCTTGATGGCT+AGG 0.481649 2:+3522073 None:intergenic
ATGGTGATGGATATTGTTGC+AGG 0.483198 2:-3522382 MsG0280006567.01.T01:CDS
TTGCATACTTGTCATGGTGA+TGG 0.517259 2:-3522395 MsG0280006567.01.T01:CDS
ATAATTGCTAACTTGCTTGG+AGG 0.540663 2:-3522013 MsG0280006567.01.T01:CDS
AGGAGAAGCCTATGAAGATG+TGG 0.600242 2:-3522114 MsG0280006567.01.T01:CDS
AGCAGAGATAGCTCAAAACA+AGG 0.658255 2:-3522344 MsG0280006567.01.T01:intron
AGCCAATAGACAATTATCTG+TGG 0.670969 2:-3521948 MsG0280006567.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATATTTATATAAACTTC+AGG + Chr2:3521906-3521925 None:intergenic 15.0%
!! AAAAATAAAGCTGATTGAAA+AGG + Chr2:3521499-3521518 None:intergenic 20.0%
!! GCAAAAAATTAAGAATAAGA+GGG + Chr2:3522079-3522098 None:intergenic 20.0%
!! TATGAATAACGTTTAAGAAT+AGG + Chr2:3522127-3522146 None:intergenic 20.0%
!! TGCAAAAAATTAAGAATAAG+AGG + Chr2:3522080-3522099 None:intergenic 20.0%
!!! CTTATTCTTAATTTTTTGCA+TGG - Chr2:3522080-3522099 MsG0280006567.01.T01:CDS 20.0%
! AATCATAGGTTTCTATTCAT+AGG - Chr2:3522300-3522319 MsG0280006567.01.T01:intron 25.0%
! ATCTATGTGTGTAATTTACA+GGG - Chr2:3522194-3522213 MsG0280006567.01.T01:intron 25.0%
! GACATTGTTTAATAAACGTT+TGG - Chr2:3521955-3521974 MsG0280006567.01.T01:CDS 25.0%
! GTGGGATATTATTTAATCAT+AGG - Chr2:3522286-3522305 MsG0280006567.01.T01:intron 25.0%
! TAATGTTCCTATAATTAGCA+TGG + Chr2:3522241-3522260 None:intergenic 25.0%
! TATCTATGTGTGTAATTTAC+AGG - Chr2:3522193-3522212 MsG0280006567.01.T01:intron 25.0%
AGATGTGGATATTTGATTGT+AGG - Chr2:3521557-3521576 MsG0280006567.01.T01:intron 30.0%
CAAGATCAAGAAAATCATGT+GGG - Chr2:3522268-3522287 MsG0280006567.01.T01:intron 30.0%
CTATTTAGACTTAGACACTT+TGG - Chr2:3521345-3521364 MsG0280006567.01.T01:CDS 30.0%
GCACTCATATATAAACGTTA+TGG + Chr2:3521764-3521783 None:intergenic 30.0%
TCAAATATCCACATCTTCAT+AGG + Chr2:3521553-3521572 None:intergenic 30.0%
TCAAGATCAAGAAAATCATG+TGG - Chr2:3522267-3522286 MsG0280006567.01.T01:intron 30.0%
TCATCGTTATTACAATCTAG+TGG + Chr2:3521937-3521956 None:intergenic 30.0%
! AATAAGGAAGCCATAGTTTT+TGG + Chr2:3521242-3521261 None:intergenic 30.0%
! ACGTAACTTTTGCAAAATCA+CGG + Chr2:3521424-3521443 None:intergenic 30.0%
! CTCTTTTGCAATATGAAAAC+AGG - Chr2:3521522-3521541 MsG0280006567.01.T01:intron 30.0%
!! CATATAATTGCTAACTTGCT+TGG - Chr2:3521640-3521659 MsG0280006567.01.T01:intron 30.0%
!! CGTTCTTTTTATTTGCCTAT+TGG - Chr2:3521812-3521831 MsG0280006567.01.T01:intron 30.0%
AGCCAATAGACAATTATCTG+TGG - Chr2:3521708-3521727 MsG0280006567.01.T01:intron 35.0%
AGCCACAGATAATTGTCTAT+TGG + Chr2:3521713-3521732 None:intergenic 35.0%
CAAGTGCATGTTTGATGTTA+CGG + Chr2:3521463-3521482 None:intergenic 35.0%
CATAGGTTTCTATTCATAGG+TGG - Chr2:3522303-3522322 MsG0280006567.01.T01:intron 35.0%
CCATGACAAGTATGCAAATA+AGG + Chr2:3521258-3521277 None:intergenic 35.0%
GCATTCTCCATGCTAATTAT+AGG - Chr2:3522231-3522250 MsG0280006567.01.T01:intron 35.0%
TGAAATAAATGCACACGTCA+TGG - Chr2:3521853-3521872 MsG0280006567.01.T01:intron 35.0%
! CCTTATTTGCATACTTGTCA+TGG - Chr2:3521255-3521274 MsG0280006567.01.T01:CDS 35.0%
! CTCATTTTCTCTTGGTAAGT+TGG - Chr2:3521736-3521755 MsG0280006567.01.T01:intron 35.0%
! TGGCTTTTCTCATTTTCTCT+TGG - Chr2:3521728-3521747 MsG0280006567.01.T01:intron 35.0%
!! ATAATTGCTAACTTGCTTGG+AGG - Chr2:3521643-3521662 MsG0280006567.01.T01:intron 35.0%
AAGGAACAGTGAACAATCCA+TGG + Chr2:3522344-3522363 None:intergenic 40.0%
AGCAGAGATAGCTCAAAACA+AGG - Chr2:3521312-3521331 MsG0280006567.01.T01:CDS 40.0%
AGCCATCAAGCTTTCCAATT+AGG - Chr2:3521586-3521605 MsG0280006567.01.T01:intron 40.0%
GATATTGTTGCAGGCATACT+TGG - Chr2:3521283-3521302 MsG0280006567.01.T01:CDS 40.0%
GCTGTGGCAGAAACATAATA+AGG + Chr2:3522363-3522382 None:intergenic 40.0%
GTCCTAATTGGAAAGCTTGA+TGG + Chr2:3521591-3521610 None:intergenic 40.0%
TAAGAGCTGCAAGTCCTAAT+TGG + Chr2:3521603-3521622 None:intergenic 40.0%
TGTAATTTACAGGGTCGTGA+TGG - Chr2:3522203-3522222 MsG0280006567.01.T01:intron 40.0%
TTGCATACTTGTCATGGTGA+TGG - Chr2:3521261-3521280 MsG0280006567.01.T01:CDS 40.0%
TTTCAAGATGTGTGACCACT+AGG + Chr2:3521838-3521857 None:intergenic 40.0%
! AATTGGAAAGCTTGATGGCT+AGG + Chr2:3521586-3521605 None:intergenic 40.0%
! GGCATTTTGTGTTTCATCCA+TGG - Chr2:3522324-3522343 MsG0280006567.01.T01:intron 40.0%
! TTATTTGCCTATTGGCCTAG+TGG - Chr2:3521820-3521839 MsG0280006567.01.T01:intron 40.0%
! TTGCCTCTTTTCTTCTCTTC+TGG + Chr2:3522388-3522407 None:intergenic 40.0%
!! ATGGTGATGGATATTGTTGC+AGG - Chr2:3521274-3521293 MsG0280006567.01.T01:CDS 40.0%
AAGAGGCAACTAGAAAGCCT+AGG - Chr2:3522399-3522418 MsG0280006567.01.T01:CDS 45.0%
AGGAGAAGCCTATGAAGATG+TGG - Chr2:3521542-3521561 MsG0280006567.01.T01:intron 45.0%
CCACCAGAAGAGAAGAAAAG+AGG - Chr2:3522382-3522401 MsG0280006567.01.T01:CDS 45.0%
GCACACGTCATGGAAAAATG+TGG - Chr2:3521863-3521882 MsG0280006567.01.T01:intron 45.0%
! CCTCTTTTCTTCTCTTCTGG+TGG + Chr2:3522385-3522404 None:intergenic 45.0%
!! GTGCATTAGACAAAAGCGTC+TGG + Chr2:3521995-3522014 None:intergenic 45.0%
TGTGTGACCACTAGGCCAAT+AGG + Chr2:3521830-3521849 None:intergenic 50.0%
TTCTTCTCTTCTGGTGGCTG+TGG + Chr2:3522379-3522398 None:intergenic 50.0%
TTGGAGGCTGCGTTTGTGTT+TGG - Chr2:3521659-3521678 MsG0280006567.01.T01:intron 50.0%
TTCGTCTTACGGCGGGACCT+AGG + Chr2:3522419-3522438 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 3521235 3522443 3521235 ID=MsG0280006567.01;Name=MsG0280006567.01
Chr2 mRNA 3521235 3522443 3521235 ID=MsG0280006567.01.T01;Parent=MsG0280006567.01;Name=MsG0280006567.01.T01;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|0|0|3|0|177
Chr2 exon 3521929 3522134 3521929 ID=MsG0280006567.01.T01:exon:802;Parent=MsG0280006567.01.T01
Chr2 exon 3522345 3522443 3522345 ID=MsG0280006567.01.T01:exon:801;Parent=MsG0280006567.01.T01
Chr2 CDS 3522345 3522443 3522345 ID=MsG0280006567.01.T01:cds;Parent=MsG0280006567.01.T01
Chr2 CDS 3521929 3522134 3521929 ID=MsG0280006567.01.T01:cds;Parent=MsG0280006567.01.T01
Chr2 CDS 3521235 3521463 3521235 ID=MsG0280006567.01.T01:cds;Parent=MsG0280006567.01.T01
Chr2 mRNA 3521235 3522125 3521235 ID=MsG0280006567.01.T02;Parent=MsG0280006567.01;Name=MsG0280006567.01.T02;_AED=0.35;_eAED=0.36;_QI=0|0|0|1|0|0|2|0|141
Chr2 exon 3521929 3522125 3521929 ID=MsG0280006567.01.T02:exon:804;Parent=MsG0280006567.01.T02
Chr2 CDS 3521929 3522125 3521929 ID=MsG0280006567.01.T02:cds;Parent=MsG0280006567.01.T02
Chr2 CDS 3521235 3521463 3521235 ID=MsG0280006567.01.T02:cds;Parent=MsG0280006567.01.T02
Gene Sequence

>MsG0280006567.01.T02

ATGAAGATGTGGATATTTGATTGTAGGAATCCTAGCCATCAAGCTTTCCAATTAGGACTTGCAGCTCTTATTCTCTTGTCTCTTGCTCATATAATTGCTAACTTGCTTGGAGGCTGCGTTTGTGTTTGGTCTAAAGATCAATATAGATCAGCTACAGCCAATAGACAATTATCTGTGGCTTTTCTCATTTTCTCTTGGGTCGTGATGGCAGTTGCATTCTCCATGCTAATTATAGGAACATTAGCAAACTCAAGATCAAGAAAATCATGTGGGATATTATTTAATCATAGGTTTCTATTCATAGGTGGCATTTTGTGTTTCATCCATGGATTGTTCACTGTTCCTTATTATGTTTCTGCCACAGCCACCAGAAGAGAAGAAAAGAGGCAACTAGAAAGCCTAGGTCCCGCCGTAAGACGAACTTAA

>MsG0280006567.01.T01

ATGGCCAAAAACTATGGCTTCCTTATTTGCATACTTGTCATGGTGATGGATATTGTTGCAGGCATACTTGGAATTCAAGCAGAGATAGCTCAAAACAAGGAGAAGCCTATGAAGATGTGGATATTTGATTGTAGGAATCCTAGCCATCAAGCTTTCCAATTAGGACTTGCAGCTCTTATTCTCTTGTCTCTTGCTCATATAATTGCTAACTTGCTTGGAGGCTGCGTTTGTGTTTGGTCTAAAGATCAATATAGATCAGCTACAGCCAATAGACAATTATCTGTGGCTTTTCTCATTTTCTCTTGGGTCGTGATGGCAGTTGCATTCTCCATGCTAATTATAGGAACATTAGCAAACTCAAGATCAAGAAAATCATGTGGGATATTATTTAATCATAGGTTTCTATTCATAGGTGGCATTTTGTGTTTCATCCATGGATTGTTCACTGTTCCTTATTATGTTTCTGCCACAGCCACCAGAAGAGAAGAAAAGAGGCAACTAGAAAGCCTAGGTCCCGCCGTAAGACGAACTTAA

Protein sequence

>MsG0280006567.01.T02

MKMWIFDCRNPSHQAFQLGLAALILLSLAHIIANLLGGCVCVWSKDQYRSATANRQLSVAFLIFSWVVMAVAFSMLIIGTLANSRSRKSCGILFNHRFLFIGGILCFIHGLFTVPYYVSATATRREEKRQLESLGPAVRRT*

>MsG0280006567.01.T01

MAKNYGFLICILVMVMDIVAGILGIQAEIAQNKEKPMKMWIFDCRNPSHQAFQLGLAALILLSLAHIIANLLGGCVCVWSKDQYRSATANRQLSVAFLIFSWVVMAVAFSMLIIGTLANSRSRKSCGILFNHRFLFIGGILCFIHGLFTVPYYVSATATRREEKRQLESLGPAVRRT*