AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004802.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004802.01.T01 MTR_1g087230 96.354 192 7 0 1 192 1 192 9.85e-139 385
MsG0180004802.01.T01 MTR_7g109410 56.345 197 76 5 1 192 1 192 4.80e-72 216
MsG0180004802.01.T01 MTR_8g107420 27.841 176 112 4 1 176 1 161 5.00e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004802.01.T01 AT4G02090 37.273 220 88 10 1 189 1 201 6.51e-32 114

Find 41 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTGAATAGGCAGATTTC+AGG 0.248523 1:+82954570 MsG0180004802.01.T01:CDS
GCCTAGCTTGGATACTATTA+AGG 0.311308 1:+82954605 MsG0180004802.01.T01:CDS
GTTCTTATAAGTTTCTTCCT+TGG 0.362756 1:+82954421 MsG0180004802.01.T01:CDS
ATCTGCCTATTCAAATAATC+TGG 0.371640 1:-82954564 None:intergenic
GGAAGAGGTGATGGAGGAGA+AGG 0.379095 1:+82954491 MsG0180004802.01.T01:CDS
TCTTCGCCGCTTAAACCAAT+AGG 0.383885 1:-82954342 None:intergenic
ATAGGCTTCCCTTGCTTCAC+TGG 0.390684 1:-82954324 None:intergenic
GGAGAATTTGCCGGACATAT+TGG 0.397303 1:+82954533 MsG0180004802.01.T01:CDS
GTGAAGCAAGGGAAGCCTAT+TGG 0.415059 1:+82954327 MsG0180004802.01.T01:CDS
TTTCGTCCTTCAGATTCAAC+AGG 0.424376 1:+82954120 MsG0180004802.01.T01:CDS
ATCTCTAGCTTCTTGTCAGC+AGG 0.426386 1:-82954279 None:intergenic
GGAGGCCAGATTATTTGAAT+AGG 0.439315 1:+82954559 MsG0180004802.01.T01:CDS
AGCTTCTTGTCAGCAGGTAA+TGG 0.466025 1:-82954273 None:intergenic
ATGTCATAACAGTGAAATAG+TGG 0.471468 1:+82954455 MsG0180004802.01.T01:CDS
GAAGAGGTGATGGAGGAGAA+GGG 0.490391 1:+82954492 MsG0180004802.01.T01:CDS
GAAAGGGTGGAAGCCTAGCT+TGG 0.506451 1:+82954593 MsG0180004802.01.T01:CDS
TCGGAGGAAGGAAGAGGTGA+TGG 0.513945 1:+82954482 MsG0180004802.01.T01:CDS
TTGTCAGCAGGTAATGGTGT+TGG 0.520247 1:-82954267 None:intergenic
ATTTGAATAGGCAGATTTCA+GGG 0.521145 1:+82954571 MsG0180004802.01.T01:CDS
AATAGGCAGATTTCAGGGAA+AGG 0.530769 1:+82954576 MsG0180004802.01.T01:CDS
GGCCTCCCTTCCAATATGTC+CGG 0.533242 1:-82954543 None:intergenic
TGATGCTTCCAGTGAAGCAA+GGG 0.535617 1:+82954316 MsG0180004802.01.T01:CDS
ATAGGCAGATTTCAGGGAAA+GGG 0.549284 1:+82954577 MsG0180004802.01.T01:CDS
AACAGTGGCAGAGCTAATGA+TGG 0.551225 1:+82954191 MsG0180004802.01.T01:CDS
GGAGCCTCGGAGGAAGGAAG+AGG 0.553960 1:+82954476 MsG0180004802.01.T01:CDS
TTGATGCTTCCAGTGAAGCA+AGG 0.559013 1:+82954315 MsG0180004802.01.T01:CDS
ACTTTACCTGTTGAATCTGA+AGG 0.560942 1:-82954126 None:intergenic
AATTTGCCGGACATATTGGA+AGG 0.563693 1:+82954537 MsG0180004802.01.T01:CDS
TCCTTAATAGTATCCAAGCT+AGG 0.569966 1:-82954606 None:intergenic
AATAGTGGAGCCTCGGAGGA+AGG 0.571841 1:+82954470 MsG0180004802.01.T01:CDS
GGGAAGCCTATTGGTTTAAG+CGG 0.581568 1:+82954336 MsG0180004802.01.T01:CDS
ACAGTGAAATAGTGGAGCCT+CGG 0.582711 1:+82954463 MsG0180004802.01.T01:CDS
GAGGAAGGAAGAGGTGATGG+AGG 0.589938 1:+82954485 MsG0180004802.01.T01:CDS
GGCAGATTTCAGGGAAAGGG+TGG 0.595138 1:+82954580 MsG0180004802.01.T01:CDS
AATTAACCATCAACAAAACT+CGG 0.602000 1:-82954371 None:intergenic
TGCCGGACATATTGGAAGGG+AGG 0.607569 1:+82954541 MsG0180004802.01.T01:CDS
GTGAAATAGTGGAGCCTCGG+AGG 0.619098 1:+82954466 MsG0180004802.01.T01:CDS
TGACATAAATTACTAAACCA+AGG 0.652368 1:-82954438 None:intergenic
ATTTGCCGGACATATTGGAA+GGG 0.657578 1:+82954538 MsG0180004802.01.T01:CDS
ATCACCTCTTCCTTCCTCCG+AGG 0.665878 1:-82954480 None:intergenic
ATTTGATGAAGCAATAACAG+TGG 0.710910 1:+82954176 MsG0180004802.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTAACCATCAACAAAACT+CGG - Chr1:82954374-82954393 None:intergenic 25.0%
! TGACATAAATTACTAAACCA+AGG - Chr1:82954441-82954460 None:intergenic 25.0%
! TTGAGAAAAAACATACTCAT+TGG + Chr1:82954637-82954656 MsG0180004802.01.T01:CDS 25.0%
ATCTGCCTATTCAAATAATC+TGG - Chr1:82954567-82954586 None:intergenic 30.0%
ATGTCATAACAGTGAAATAG+TGG + Chr1:82954455-82954474 MsG0180004802.01.T01:CDS 30.0%
ATTTGAATAGGCAGATTTCA+GGG + Chr1:82954571-82954590 MsG0180004802.01.T01:CDS 30.0%
ATTTGATGAAGCAATAACAG+TGG + Chr1:82954176-82954195 MsG0180004802.01.T01:CDS 30.0%
GTTCTTATAAGTTTCTTCCT+TGG + Chr1:82954421-82954440 MsG0180004802.01.T01:CDS 30.0%
TATTTGAATAGGCAGATTTC+AGG + Chr1:82954570-82954589 MsG0180004802.01.T01:CDS 30.0%
! GGTAAAGTAGTTTTCTTAGA+TGG + Chr1:82954141-82954160 MsG0180004802.01.T01:CDS 30.0%
ACTTTACCTGTTGAATCTGA+AGG - Chr1:82954129-82954148 None:intergenic 35.0%
ATTCAGCTGTGAACCAAAAA+AGG + Chr1:82954244-82954263 MsG0180004802.01.T01:CDS 35.0%
TCCTTAATAGTATCCAAGCT+AGG - Chr1:82954609-82954628 None:intergenic 35.0%
!!! AACTCAACCACTACTTTTTG+TGG - Chr1:82954222-82954241 None:intergenic 35.0%
AATAGGCAGATTTCAGGGAA+AGG + Chr1:82954576-82954595 MsG0180004802.01.T01:CDS 40.0%
AATTTGCCGGACATATTGGA+AGG + Chr1:82954537-82954556 MsG0180004802.01.T01:CDS 40.0%
ATAGGCAGATTTCAGGGAAA+GGG + Chr1:82954577-82954596 MsG0180004802.01.T01:CDS 40.0%
ATTTGCCGGACATATTGGAA+GGG + Chr1:82954538-82954557 MsG0180004802.01.T01:CDS 40.0%
GCCTAGCTTGGATACTATTA+AGG + Chr1:82954605-82954624 MsG0180004802.01.T01:CDS 40.0%
GGAGGCCAGATTATTTGAAT+AGG + Chr1:82954559-82954578 MsG0180004802.01.T01:CDS 40.0%
GGATCATCCACAAAAAGTAG+TGG + Chr1:82954212-82954231 MsG0180004802.01.T01:CDS 40.0%
TTTCGTCCTTCAGATTCAAC+AGG + Chr1:82954120-82954139 MsG0180004802.01.T01:CDS 40.0%
!! CGATTTTTCGGAGAATTTGC+CGG + Chr1:82954524-82954543 MsG0180004802.01.T01:CDS 40.0%
!!! GTAATGGTGTTGGCCTTTTT+TGG - Chr1:82954260-82954279 None:intergenic 40.0%
AACAGTGGCAGAGCTAATGA+TGG + Chr1:82954191-82954210 MsG0180004802.01.T01:CDS 45.0%
AGCTTCTTGTCAGCAGGTAA+TGG - Chr1:82954276-82954295 None:intergenic 45.0%
GGAGAATTTGCCGGACATAT+TGG + Chr1:82954533-82954552 MsG0180004802.01.T01:CDS 45.0%
TCTTCGCCGCTTAAACCAAT+AGG - Chr1:82954345-82954364 None:intergenic 45.0%
TGATGCTTCCAGTGAAGCAA+GGG + Chr1:82954316-82954335 MsG0180004802.01.T01:CDS 45.0%
TTGATGCTTCCAGTGAAGCA+AGG + Chr1:82954315-82954334 MsG0180004802.01.T01:CDS 45.0%
! ACAGTGAAATAGTGGAGCCT+CGG + Chr1:82954463-82954482 MsG0180004802.01.T01:CDS 45.0%
! ATCTCTAGCTTCTTGTCAGC+AGG - Chr1:82954282-82954301 None:intergenic 45.0%
! GGGAGAGAGACACGATTTTT+CGG + Chr1:82954512-82954531 MsG0180004802.01.T01:CDS 45.0%
! TTGTCAGCAGGTAATGGTGT+TGG - Chr1:82954270-82954289 None:intergenic 45.0%
!! GGGAAGCCTATTGGTTTAAG+CGG + Chr1:82954336-82954355 MsG0180004802.01.T01:CDS 45.0%
!!! AACTCGCCGAGTTTTGTTGA+TGG + Chr1:82954365-82954384 MsG0180004802.01.T01:CDS 45.0%
ATAGGCTTCCCTTGCTTCAC+TGG - Chr1:82954327-82954346 None:intergenic 50.0%
GTGAAGCAAGGGAAGCCTAT+TGG + Chr1:82954327-82954346 MsG0180004802.01.T01:CDS 50.0%
!! GAAGAGGTGATGGAGGAGAA+GGG + Chr1:82954492-82954511 MsG0180004802.01.T01:CDS 50.0%
ATCACCTCTTCCTTCCTCCG+AGG - Chr1:82954483-82954502 None:intergenic 55.0%
GAAAGGGTGGAAGCCTAGCT+TGG + Chr1:82954593-82954612 MsG0180004802.01.T01:CDS 55.0%
GGCAGATTTCAGGGAAAGGG+TGG + Chr1:82954580-82954599 MsG0180004802.01.T01:CDS 55.0%
GGCCTCCCTTCCAATATGTC+CGG - Chr1:82954546-82954565 None:intergenic 55.0%
TCGGAGGAAGGAAGAGGTGA+TGG + Chr1:82954482-82954501 MsG0180004802.01.T01:CDS 55.0%
TGCCGGACATATTGGAAGGG+AGG + Chr1:82954541-82954560 MsG0180004802.01.T01:CDS 55.0%
! AATAGTGGAGCCTCGGAGGA+AGG + Chr1:82954470-82954489 MsG0180004802.01.T01:CDS 55.0%
! GAGGAAGGAAGAGGTGATGG+AGG + Chr1:82954485-82954504 MsG0180004802.01.T01:CDS 55.0%
! GTGAAATAGTGGAGCCTCGG+AGG + Chr1:82954466-82954485 MsG0180004802.01.T01:CDS 55.0%
!! GGAAGAGGTGATGGAGGAGA+AGG + Chr1:82954491-82954510 MsG0180004802.01.T01:CDS 55.0%
GGAGCCTCGGAGGAAGGAAG+AGG + Chr1:82954476-82954495 MsG0180004802.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 82954102 82954680 82954102 ID=MsG0180004802.01;Name=MsG0180004802.01
Chr1 mRNA 82954102 82954680 82954102 ID=MsG0180004802.01.T01;Parent=MsG0180004802.01;Name=MsG0180004802.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|192
Chr1 exon 82954102 82954680 82954102 ID=MsG0180004802.01.T01:exon:27474;Parent=MsG0180004802.01.T01
Chr1 CDS 82954102 82954680 82954102 ID=MsG0180004802.01.T01:cds;Parent=MsG0180004802.01.T01
Gene Sequence

>MsG0180004802.01.T01

ATGGGAAATCAAGTCTCATTTCGTCCTTCAGATTCAACAGGTAAAGTAGTTTTCTTAGATGGTTCAGTTCATGAATTTGATGAAGCAATAACAGTGGCAGAGCTAATGATGGATCATCCACAAAAAGTAGTGGTTGAGTTTCATTCAGCTGTGAACCAAAAAAGGCCAACACCATTACCTGCTGACAAGAAGCTAGAGATGAACAAGATATATTTGATGCTTCCAGTGAAGCAAGGGAAGCCTATTGGTTTAAGCGGCGAAGAAACTCGCCGAGTTTTGTTGATGGTTAATTCTATTCTGAATTCTAATTATGTTTTGTGTTCTTATAAGTTTCTTCCTTGGTTTAGTAATTTATGTCATAACAGTGAAATAGTGGAGCCTCGGAGGAAGGAAGAGGTGATGGAGGAGAAGGGAGAGAGACACGATTTTTCGGAGAATTTGCCGGACATATTGGAAGGGAGGCCAGATTATTTGAATAGGCAGATTTCAGGGAAAGGGTGGAAGCCTAGCTTGGATACTATTAAGGAGAAGAATATTGAGAAAAAACATACTCATTGGTTGTTTCTCAAGAGTTTTTAG

Protein sequence

>MsG0180004802.01.T01

MGNQVSFRPSDSTGKVVFLDGSVHEFDEAITVAELMMDHPQKVVVEFHSAVNQKRPTPLPADKKLEMNKIYLMLPVKQGKPIGLSGEETRRVLLMVNSILNSNYVLCSYKFLPWFSNLCHNSEIVEPRRKEEVMEEKGERHDFSENLPDILEGRPDYLNRQISGKGWKPSLDTIKEKNIEKKHTHWLFLKSF*