Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036925.01.T01 | XP_003621788.1 | 99.25 | 400 | 3 | 0 | 1 | 400 | 1 | 400 | 0 | 828 |
| MsG0780036925.01.T02 | XP_003621788.1 | 99.25 | 400 | 3 | 0 | 1 | 400 | 1 | 400 | 0 | 828 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047831.01 | MsG0780036925.01 | -0.806539 | 7.833434e-50 | 4.262776e-47 |
| MsG0080047967.01 | MsG0780036925.01 | 0.834114 | 3.613110e-56 | 4.246290e-53 |
| MsG0080048059.01 | MsG0780036925.01 | 0.847268 | 1.287471e-59 | 2.275845e-56 |
| MsG0080048560.01 | MsG0780036925.01 | 0.821854 | 3.234728e-53 | 2.662900e-50 |
| MsG0080048813.01 | MsG0780036925.01 | 0.884969 | 1.217352e-71 | 8.203617e-68 |
| MsG0080048896.01 | MsG0780036925.01 | 0.816791 | 4.608237e-52 | 3.297038e-49 |
| MsG0080048909.01 | MsG0780036925.01 | 0.847071 | 1.457890e-59 | 2.561131e-56 |
| MsG0080048992.01 | MsG0780036925.01 | 0.833753 | 4.449074e-56 | 5.172242e-53 |
| MsG0080049015.01 | MsG0780036925.01 | 0.827123 | 1.861183e-54 | 1.780277e-51 |
| MsG0080049016.01 | MsG0780036925.01 | 0.853334 | 2.559115e-61 | 5.510581e-58 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880044106.01 | MsG0780036925.01 | PPI |
| MsG0880044106.01 | MsG0780036925.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036925.01.T01 | MTR_7g022890 | 99.250 | 400 | 3 | 0 | 1 | 400 | 1 | 400 | 0.0 | 828 |
| MsG0780036925.01.T01 | MTR_7g022890 | 99.410 | 339 | 2 | 0 | 62 | 400 | 9 | 347 | 0.0 | 701 |
| MsG0780036925.01.T01 | MTR_8g046550 | 60.733 | 382 | 140 | 5 | 21 | 395 | 12 | 390 | 9.89e-161 | 457 |
| MsG0780036925.01.T01 | MTR_2g064405 | 62.133 | 375 | 134 | 5 | 25 | 395 | 19 | 389 | 2.67e-159 | 454 |
| MsG0780036925.01.T01 | MTR_2g064405 | 66.454 | 313 | 103 | 2 | 84 | 395 | 1 | 312 | 1.18e-146 | 419 |
| MsG0780036925.01.T01 | MTR_3g080090 | 57.219 | 374 | 146 | 4 | 29 | 397 | 33 | 397 | 1.63e-144 | 416 |
| MsG0780036925.01.T01 | MTR_3g062610 | 57.317 | 328 | 135 | 2 | 69 | 396 | 72 | 394 | 7.37e-130 | 379 |
| MsG0780036925.01.T01 | MTR_5g075680 | 56.098 | 328 | 139 | 3 | 69 | 396 | 12 | 334 | 3.80e-126 | 367 |
| MsG0780036925.01.T01 | MTR_5g075680 | 56.098 | 328 | 139 | 3 | 69 | 396 | 65 | 387 | 9.12e-126 | 369 |
| MsG0780036925.01.T01 | MTR_3g062610 | 50.000 | 258 | 119 | 3 | 69 | 326 | 72 | 319 | 4.38e-83 | 257 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036925.01.T01 | AT2G39450 | 81.313 | 396 | 72 | 1 | 5 | 400 | 1 | 394 | 0.0 | 608 |
| MsG0780036925.01.T01 | AT1G16310 | 59.898 | 394 | 138 | 5 | 13 | 395 | 41 | 425 | 4.91e-164 | 467 |
| MsG0780036925.01.T01 | AT1G79520 | 62.632 | 380 | 128 | 4 | 24 | 395 | 25 | 398 | 3.84e-163 | 464 |
| MsG0780036925.01.T01 | AT1G79520 | 61.240 | 387 | 134 | 5 | 24 | 395 | 25 | 410 | 8.28e-163 | 463 |
| MsG0780036925.01.T01 | AT1G79520 | 67.066 | 334 | 108 | 2 | 63 | 395 | 13 | 345 | 1.24e-162 | 461 |
| MsG0780036925.01.T01 | AT1G79520 | 67.066 | 334 | 108 | 2 | 63 | 395 | 13 | 345 | 1.24e-162 | 461 |
| MsG0780036925.01.T01 | AT1G16310 | 66.773 | 313 | 102 | 2 | 84 | 395 | 1 | 312 | 1.64e-150 | 429 |
| MsG0780036925.01.T01 | AT3G58060 | 53.977 | 352 | 157 | 3 | 50 | 396 | 51 | 402 | 4.98e-127 | 372 |
| MsG0780036925.01.T01 | AT3G58060 | 53.977 | 352 | 157 | 3 | 50 | 396 | 51 | 402 | 4.98e-127 | 372 |
| MsG0780036925.01.T02 | AT2G39450 | 81.313 | 396 | 72 | 1 | 5 | 400 | 1 | 394 | 0.0 | 608 |
Find 31 sgRNAs with CRISPR-Local
Find 361 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACATGATACTTAGACATT+AGG | 0.407688 | 7:-16606438 | None:intergenic |
| AAGACACATTGACACTGTTC+GGG | 0.476544 | 7:+16606018 | MsG0780036925.01.T01:CDS |
| AATAAACTTCCTTACATGAC+AGG | 0.520941 | 7:-16606357 | None:intergenic |
| ACAAAAGCACGCTCGATCTC+AGG | 0.463499 | 7:-16606157 | None:intergenic |
| ACTGTTCGGGCATATACATT+CGG | 0.522461 | 7:+16606031 | MsG0780036925.01.T01:CDS |
| AGAAACTTACATACCTCTGC+TGG | 0.477384 | 7:+16605978 | MsG0780036925.01.T01:CDS |
| AGCAGACATGCCTTTGCAAG+AGG | 0.566228 | 7:+16606090 | MsG0780036925.01.T01:CDS |
| ATATCATGAGCCTCTTGCAA+AGG | 0.600551 | 7:-16606100 | None:intergenic |
| ATCTAAAACTAGAATATCAG+AGG | 0.606587 | 7:+16606465 | MsG0780036925.01.T01:intron |
| ATGATACTTAGACATTAGGT+AGG | 0.538457 | 7:-16606434 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATTGTGTAATTGTGTGC+AGG | + | Chr7:16603810-16603829 | MsG0780036925.01.T01:intron | 30.0% | |
| AAATTGTGTAATTGTGTGCA+GGG | + | Chr7:16603811-16603830 | MsG0780036925.01.T01:intron | 30.0% | |
| AAGAAGAAATCTCTCATTAC+AGG | + | Chr7:16605499-16605518 | MsG0780036925.01.T01:intron | 30.0% | |
| AATAAACTTCCTTACATGAC+AGG | - | Chr7:16606360-16606379 | None:intergenic | 30.0% | |
| AATAGAAACCTTTGACATTC+CGG | - | Chr7:16603075-16603094 | None:intergenic | 30.0% | |
| AATATCGAACATAGCTAACA+TGG | + | Chr7:16603508-16603527 | MsG0780036925.01.T01:CDS | 30.0% | |
| AATCTTAGCAGTCAAGAAAA+TGG | - | Chr7:16605722-16605741 | None:intergenic | 30.0% | |
| ACCAATTTGGATATTTACTC+AGG | + | Chr7:16602050-16602069 | MsG0780036925.01.T01:intron | 30.0% | |
| ACCATAAGATAAATCCAGAA+AGG | - | Chr7:16603619-16603638 | None:intergenic | 30.0% | |
| AGCTGTTAATAATAAAGTGG+GGG | + | Chr7:16601935-16601954 | MsG0780036925.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 16600768 | 16606604 | 16600768 | ID=MsG0780036925.01;Name=MsG0780036925.01 |
| Chr7 | mRNA | 16600768 | 16606604 | 16600768 | ID=MsG0780036925.01.T01;Parent=MsG0780036925.01;Name=MsG0780036925.01.T01;_AED=0.39;_eAED=0.39;_QI=132|1|1|1|0.83|0.71|7|308|400 |
| Chr7 | exon | 16605887 | 16606479 | 16605887 | ID=MsG0780036925.01.T01:exon:11396;Parent=MsG0780036925.01.T01 |
| Chr7 | exon | 16606542 | 16606604 | 16606542 | ID=MsG0780036925.01.T01:exon:11397;Parent=MsG0780036925.01.T01 |
| Chr7 | five_prime_UTR | 16600768 | 16600899 | 16600768 | ID=MsG0780036925.01.T01:five_prime_utr;Parent=MsG0780036925.01.T01 |
| Chr7 | CDS | 16600900 | 16601083 | 16600900 | ID=MsG0780036925.01.T01:cds;Parent=MsG0780036925.01.T01 |
| Chr7 | CDS | 16602976 | 16603085 | 16602976 | ID=MsG0780036925.01.T01:cds;Parent=MsG0780036925.01.T01 |
| Chr7 | CDS | 16603464 | 16603709 | 16603464 | ID=MsG0780036925.01.T01:cds;Parent=MsG0780036925.01.T01 |
| Chr7 | CDS | 16603832 | 16603912 | 16603832 | ID=MsG0780036925.01.T01:cds;Parent=MsG0780036925.01.T01 |
| Chr7 | CDS | 16604034 | 16604267 | 16604034 | ID=MsG0780036925.01.T01:cds;Parent=MsG0780036925.01.T01 |
| Chr7 | CDS | 16605887 | 16606234 | 16605887 | ID=MsG0780036925.01.T01:cds;Parent=MsG0780036925.01.T01 |
| Chr7 | three_prime_UTR | 16606235 | 16606479 | 16606235 | ID=MsG0780036925.01.T01:three_prime_utr;Parent=MsG0780036925.01.T01 |
| Chr7 | three_prime_UTR | 16606542 | 16606604 | 16606542 | ID=MsG0780036925.01.T01:three_prime_utr;Parent=MsG0780036925.01.T01 |
| Chr7 | mRNA | 16600768 | 16606500 | 16600768 | ID=MsG0780036925.01.T02;Parent=MsG0780036925.01;Name=MsG0780036925.01.T02;_AED=0.39;_eAED=0.39;_QI=132|1|1|1|1|1|6|266|400 |
| Chr7 | exon | 16605887 | 16606500 | 16605887 | ID=MsG0780036925.01.T02:exon:11398;Parent=MsG0780036925.01.T02 |
| Chr7 | five_prime_UTR | 16600768 | 16600899 | 16600768 | ID=MsG0780036925.01.T02:five_prime_utr;Parent=MsG0780036925.01.T02 |
| Chr7 | CDS | 16600900 | 16601083 | 16600900 | ID=MsG0780036925.01.T02:cds;Parent=MsG0780036925.01.T02 |
| Chr7 | CDS | 16602976 | 16603085 | 16602976 | ID=MsG0780036925.01.T02:cds;Parent=MsG0780036925.01.T02 |
| Chr7 | CDS | 16603464 | 16603709 | 16603464 | ID=MsG0780036925.01.T02:cds;Parent=MsG0780036925.01.T02 |
| Chr7 | CDS | 16603832 | 16603912 | 16603832 | ID=MsG0780036925.01.T02:cds;Parent=MsG0780036925.01.T02 |
| Chr7 | CDS | 16604034 | 16604267 | 16604034 | ID=MsG0780036925.01.T02:cds;Parent=MsG0780036925.01.T02 |
| Chr7 | CDS | 16605887 | 16606234 | 16605887 | ID=MsG0780036925.01.T02:cds;Parent=MsG0780036925.01.T02 |
| Chr7 | three_prime_UTR | 16606235 | 16606500 | 16606235 | ID=MsG0780036925.01.T02:three_prime_utr;Parent=MsG0780036925.01.T02 |
| Gene Sequence |
>MsG0780036925.01.T01 ATGACCGGGAAAATGGTGGAGACGGTGGAGCAACCTAACGAAGAAGAGCTTTCTCTTCTTTCAGTTTCAGACAATGGTGACCGGTCATGGCGGTTGAACTTCGACTCTTTTCAGTTGAATTCTCAGCATACTGAAAAGACACCTAAACCCTCTTCTGGTATTCATGATTGCTATGGTGTTCTTGGTCAAGAAGATAATATTGCTGAGTACTATCAGCAGCAGGTTGAAGTGCTTGAAGGCTTTACCGAAATGGATGCCTTAACAGAGCGTGGTTTCCTTCCCGGAATGTCAAAGGAGGAGCGAGATAAGTTAGCCAGGAGCGAGACGTTTGCCATCAGAATATCGAACATAGCTAACATGGTTCTTTTTACTGCCAAAGTTTATGCATCAGTCAGAAGTGGCTCCCTAGCCATCATTGCATCCACTTTAGACTCTCTTCTGGACCTCCTTTCTGGATTTATCTTATGGTTCACTGCATTTTCCATGCAAACACCAAATCCGTATCAGTACCCTATTGGAAAGAAGCGGATGCAACCATTGGGAATTCTGGTTTTTGCCTCTGTCATGGCGACCCTAGGGCTGCAAATAATTTTGGAGTCTGCCCGCACACTAATATACACTGATAATACCTTCAGCTTGACTAGGGAACAAGAGCGCTGGGTTGTGGGTATTATGCTTTCAGTGACTTTGGTGAAATTCATGCTAATGATTTATTGCCGCTCTTTTACTAATGAGATTGTGAAGGCCTATGCTCAGGATCACTTTTTTGATGTGATCACTAATGTGATTGGTCTAATTGCTGCACTTTTGGCCAATTATTTTGATGATTGGATGGATCCTGTTGGTGCTATCATTCTGGCTTTGTACACAATTCGCACATGGTCAATGACAGTGTTGGAAAATGTGAATTCACTTGTTGGAAGATCAGCTGCACCAGAGTATCTTCAGAAACTTACATACCTCTGCTGGAACCACCACAAGGCTGTAAGACACATTGACACTGTTCGGGCATATACATTCGGCTCTCACTACTTTGTCGAGGTTGATATCGTTCTACCAGCAGACATGCCTTTGCAAGAGGCTCATGATATTGGGGAATCATTGCAAGAGAAGCTCGAACTTTTGCCTGAGATCGAGCGTGCTTTTGTTCATCTTGATTATGAATTCAGTCACAAACCTGAGCATGCACAAGCACATTCTTAG >MsG0780036925.01.T02 ATGACCGGGAAAATGGTGGAGACGGTGGAGCAACCTAACGAAGAAGAGCTTTCTCTTCTTTCAGTTTCAGACAATGGTGACCGGTCATGGCGGTTGAACTTCGACTCTTTTCAGTTGAATTCTCAGCATACTGAAAAGACACCTAAACCCTCTTCTGGTATTCATGATTGCTATGGTGTTCTTGGTCAAGAAGATAATATTGCTGAGTACTATCAGCAGCAGGTTGAAGTGCTTGAAGGCTTTACCGAAATGGATGCCTTAACAGAGCGTGGTTTCCTTCCCGGAATGTCAAAGGAGGAGCGAGATAAGTTAGCCAGGAGCGAGACGTTTGCCATCAGAATATCGAACATAGCTAACATGGTTCTTTTTACTGCCAAAGTTTATGCATCAGTCAGAAGTGGCTCCCTAGCCATCATTGCATCCACTTTAGACTCTCTTCTGGACCTCCTTTCTGGATTTATCTTATGGTTCACTGCATTTTCCATGCAAACACCAAATCCGTATCAGTACCCTATTGGAAAGAAGCGGATGCAACCATTGGGAATTCTGGTTTTTGCCTCTGTCATGGCGACCCTAGGGCTGCAAATAATTTTGGAGTCTGCCCGCACACTAATATACACTGATAATACCTTCAGCTTGACTAGGGAACAAGAGCGCTGGGTTGTGGGTATTATGCTTTCAGTGACTTTGGTGAAATTCATGCTAATGATTTATTGCCGCTCTTTTACTAATGAGATTGTGAAGGCCTATGCTCAGGATCACTTTTTTGATGTGATCACTAATGTGATTGGTCTAATTGCTGCACTTTTGGCCAATTATTTTGATGATTGGATGGATCCTGTTGGTGCTATCATTCTGGCTTTGTACACAATTCGCACATGGTCAATGACAGTGTTGGAAAATGTGAATTCACTTGTTGGAAGATCAGCTGCACCAGAGTATCTTCAGAAACTTACATACCTCTGCTGGAACCACCACAAGGCTGTAAGACACATTGACACTGTTCGGGCATATACATTCGGCTCTCACTACTTTGTCGAGGTTGATATCGTTCTACCAGCAGACATGCCTTTGCAAGAGGCTCATGATATTGGGGAATCATTGCAAGAGAAGCTCGAACTTTTGCCTGAGATCGAGCGTGCTTTTGTTCATCTTGATTATGAATTCAGTCACAAACCTGAGCATGCACAAGCACATTCTTAG |
| Protein sequence |
>MsG0780036925.01.T01 MTGKMVETVEQPNEEELSLLSVSDNGDRSWRLNFDSFQLNSQHTEKTPKPSSGIHDCYGVLGQEDNIAEYYQQQVEVLEGFTEMDALTERGFLPGMSKEERDKLARSETFAIRISNIANMVLFTAKVYASVRSGSLAIIASTLDSLLDLLSGFILWFTAFSMQTPNPYQYPIGKKRMQPLGILVFASVMATLGLQIILESARTLIYTDNTFSLTREQERWVVGIMLSVTLVKFMLMIYCRSFTNEIVKAYAQDHFFDVITNVIGLIAALLANYFDDWMDPVGAIILALYTIRTWSMTVLENVNSLVGRSAAPEYLQKLTYLCWNHHKAVRHIDTVRAYTFGSHYFVEVDIVLPADMPLQEAHDIGESLQEKLELLPEIERAFVHLDYEFSHKPEHAQAHS* >MsG0780036925.01.T02 MTGKMVETVEQPNEEELSLLSVSDNGDRSWRLNFDSFQLNSQHTEKTPKPSSGIHDCYGVLGQEDNIAEYYQQQVEVLEGFTEMDALTERGFLPGMSKEERDKLARSETFAIRISNIANMVLFTAKVYASVRSGSLAIIASTLDSLLDLLSGFILWFTAFSMQTPNPYQYPIGKKRMQPLGILVFASVMATLGLQIILESARTLIYTDNTFSLTREQERWVVGIMLSVTLVKFMLMIYCRSFTNEIVKAYAQDHFFDVITNVIGLIAALLANYFDDWMDPVGAIILALYTIRTWSMTVLENVNSLVGRSAAPEYLQKLTYLCWNHHKAVRHIDTVRAYTFGSHYFVEVDIVLPADMPLQEAHDIGESLQEKLELLPEIERAFVHLDYEFSHKPEHAQAHS* |
