AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780035932.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780035932.01.T01 MTR_7g405740 81.212 165 27 2 1 164 1 162 1.66e-90 261
MsG0780035932.01.T01 MTR_3g098970 37.903 124 67 3 11 134 5 118 5.04e-19 79.0
MsG0780035932.01.T01 MTR_8g096500 52.727 55 22 1 93 143 73 127 2.47e-12 61.6
MsG0780035932.01.T01 MTR_8g096440 52.727 55 22 1 93 143 69 123 2.99e-12 61.2
MsG0780035932.01.T01 MTR_8g096470 56.098 41 18 0 93 133 70 110 2.42e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780035932.01.T01 AT5G50760 37.719 114 71 0 19 132 5 118 4.37e-17 74.7

Find 40 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATCTTCTTAGCCCTTCC+AGG 0.273002 7:+257510 MsG0780035932.01.T01:CDS
CCTTCCCAAGGTTGTACTTT+TGG 0.273068 7:+257470 MsG0780035932.01.T01:CDS
ACCACAAACCTTTGCATTTG+AGG 0.340829 7:-257300 None:intergenic
GGAAAAGGGAACAAGAAAAT+AGG 0.347522 7:+257089 MsG0780035932.01.T01:CDS
GAAGAAGCTGAATCAGAATA+TGG 0.364921 7:+257365 MsG0780035932.01.T01:CDS
TTACAAAGTGTTGATGGAAA+TGG 0.371199 7:+257436 MsG0780035932.01.T01:CDS
TTATAGCTGCCAAGGACCTT+TGG 0.404368 7:+257388 MsG0780035932.01.T01:CDS
AAGAACATGAAGCAAGGAAA+AGG 0.428717 7:+257074 MsG0780035932.01.T01:CDS
ACCTCAAATGCAAAGGTTTG+TGG 0.451149 7:+257299 MsG0780035932.01.T01:CDS
GTTGTTTAAGACAATCATCC+TGG 0.454314 7:-257528 None:intergenic
GATTTGCTCCTTCTTGTCGT+TGG 0.461730 7:-257200 None:intergenic
AATCATGGCCTAGTCTTCGC+GGG 0.492731 7:+257219 MsG0780035932.01.T01:CDS
TAAAAGACATCAACATTACA+AGG 0.510903 7:-257416 None:intergenic
AGAACATGAAGCAAGGAAAA+GGG 0.512113 7:+257075 MsG0780035932.01.T01:CDS
TGCAAGTCCATAGGAGGAGG+AGG 0.512745 7:+257137 MsG0780035932.01.T01:CDS
TTCTTCGCCCCGCGAAGACT+AGG 0.514969 7:-257227 None:intergenic
AAATCATGGCCTAGTCTTCG+CGG 0.516881 7:+257218 MsG0780035932.01.T01:CDS
AAGGAAGAGCCAAAGGTCCT+TGG 0.517712 7:-257397 None:intergenic
CGACAAGAAGGAGCAAATCA+TGG 0.519386 7:+257204 MsG0780035932.01.T01:CDS
ATAGACAAAGAAAGTGGCCC+CGG 0.527502 7:+257251 MsG0780035932.01.T01:CDS
TCAAGCAACATCTTGAACAA+AGG 0.532123 7:-257344 None:intergenic
ACGACCAAAAGTACAACCTT+GGG 0.533998 7:-257474 None:intergenic
AACGACCAAAAGTACAACCT+TGG 0.538821 7:-257475 None:intergenic
AGATGCAAGTCCATAGGAGG+AGG 0.541895 7:+257134 MsG0780035932.01.T01:CDS
AAGTCCATAGGAGGAGGAGG+AGG 0.549753 7:+257140 MsG0780035932.01.T01:CDS
GAATATGGTTATAGCTGCCA+AGG 0.552741 7:+257380 MsG0780035932.01.T01:CDS
CAGTGATTCCAACGACAAGA+AGG 0.563887 7:+257192 MsG0780035932.01.T01:CDS
TTTAAGACAATCATCCTGGA+AGG 0.580942 7:-257524 None:intergenic
AAGAAAATAGACAAAGAAAG+TGG 0.596302 7:+257245 MsG0780035932.01.T01:CDS
TGGGAGAGATGCAAGTCCAT+AGG 0.597578 7:+257128 MsG0780035932.01.T01:CDS
GAGAGATGCAAGTCCATAGG+AGG 0.601664 7:+257131 MsG0780035932.01.T01:CDS
ACAAAGAAAGTGGCCCCGGA+GGG 0.607143 7:+257255 MsG0780035932.01.T01:CDS
TTAAGACAATCATCCTGGAA+GGG 0.611880 7:-257523 None:intergenic
ACATTACAAGGAAGAGCCAA+AGG 0.626356 7:-257404 None:intergenic
ACGTTGGACCTCAAATGCAA+AGG 0.634178 7:+257292 MsG0780035932.01.T01:CDS
ATTGGCAAGAACATGAAGCA+AGG 0.634830 7:+257068 MsG0780035932.01.T01:CDS
GATAACGAAGCTCCTTCCCA+AGG 0.635029 7:+257458 MsG0780035932.01.T01:CDS
GACAAAGAAAGTGGCCCCGG+AGG 0.643163 7:+257254 MsG0780035932.01.T01:CDS
CCAAAAGTACAACCTTGGGA+AGG 0.674764 7:-257470 None:intergenic
ATCATGGCCTAGTCTTCGCG+GGG 0.686876 7:+257220 MsG0780035932.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGAAAATAGACAAAGAAAG+TGG + Chr7:257245-257264 MsG0780035932.01.T01:CDS 25.0%
! TAAAAGACATCAACATTACA+AGG - Chr7:257419-257438 None:intergenic 25.0%
!! TTACAAAGTGTTGATGGAAA+TGG + Chr7:257436-257455 MsG0780035932.01.T01:CDS 30.0%
!!! TGTCTTTTACAAAGTGTTGA+TGG + Chr7:257430-257449 MsG0780035932.01.T01:CDS 30.0%
AAGAACATGAAGCAAGGAAA+AGG + Chr7:257074-257093 MsG0780035932.01.T01:CDS 35.0%
AGAACATGAAGCAAGGAAAA+GGG + Chr7:257075-257094 MsG0780035932.01.T01:CDS 35.0%
GAAGAAGCTGAATCAGAATA+TGG + Chr7:257365-257384 MsG0780035932.01.T01:CDS 35.0%
GGAAAAGGGAACAAGAAAAT+AGG + Chr7:257089-257108 MsG0780035932.01.T01:CDS 35.0%
TCAAGCAACATCTTGAACAA+AGG - Chr7:257347-257366 None:intergenic 35.0%
TTAAGACAATCATCCTGGAA+GGG - Chr7:257526-257545 None:intergenic 35.0%
! GTTGTTTAAGACAATCATCC+TGG - Chr7:257531-257550 None:intergenic 35.0%
! TTTAAGACAATCATCCTGGA+AGG - Chr7:257527-257546 None:intergenic 35.0%
ACATTACAAGGAAGAGCCAA+AGG - Chr7:257407-257426 None:intergenic 40.0%
ACCACAAACCTTTGCATTTG+AGG - Chr7:257303-257322 None:intergenic 40.0%
ACCTCAAATGCAAAGGTTTG+TGG + Chr7:257299-257318 MsG0780035932.01.T01:CDS 40.0%
AGGCCTCATAACAAAAACGT+GGG + Chr7:257109-257128 MsG0780035932.01.T01:CDS 40.0%
ATTGGCAAGAACATGAAGCA+AGG + Chr7:257068-257087 MsG0780035932.01.T01:CDS 40.0%
GAATATGGTTATAGCTGCCA+AGG + Chr7:257380-257399 MsG0780035932.01.T01:CDS 40.0%
TAGGCCTCATAACAAAAACG+TGG + Chr7:257108-257127 MsG0780035932.01.T01:CDS 40.0%
! AACGACCAAAAGTACAACCT+TGG - Chr7:257478-257497 None:intergenic 40.0%
! ACGACCAAAAGTACAACCTT+GGG - Chr7:257477-257496 None:intergenic 40.0%
!! TCTCCCACGTTTTTGTTATG+AGG - Chr7:257115-257134 None:intergenic 40.0%
ACGTTGGACCTCAAATGCAA+AGG + Chr7:257292-257311 MsG0780035932.01.T01:CDS 45.0%
ATAGACAAAGAAAGTGGCCC+CGG + Chr7:257251-257270 MsG0780035932.01.T01:CDS 45.0%
ATCATCTTCTTAGCCCTTCC+AGG + Chr7:257510-257529 MsG0780035932.01.T01:CDS 45.0%
CAGTGATTCCAACGACAAGA+AGG + Chr7:257192-257211 MsG0780035932.01.T01:CDS 45.0%
CGACAAGAAGGAGCAAATCA+TGG + Chr7:257204-257223 MsG0780035932.01.T01:CDS 45.0%
TACACCGAAAAACATCCCTC+CGG - Chr7:257273-257292 None:intergenic 45.0%
TTATAGCTGCCAAGGACCTT+TGG + Chr7:257388-257407 MsG0780035932.01.T01:CDS 45.0%
! AAATCATGGCCTAGTCTTCG+CGG + Chr7:257218-257237 MsG0780035932.01.T01:CDS 45.0%
! CCAAAAGTACAACCTTGGGA+AGG - Chr7:257473-257492 None:intergenic 45.0%
! CCTTCCCAAGGTTGTACTTT+TGG + Chr7:257470-257489 MsG0780035932.01.T01:CDS 45.0%
! GATTTGCTCCTTCTTGTCGT+TGG - Chr7:257203-257222 None:intergenic 45.0%
!! GGATGTTTTTCGGTGTACGT+TGG + Chr7:257276-257295 MsG0780035932.01.T01:CDS 45.0%
AAGGAAGAGCCAAAGGTCCT+TGG - Chr7:257400-257419 None:intergenic 50.0%
ACACCGAAAAACATCCCTCC+GGG - Chr7:257272-257291 None:intergenic 50.0%
AGATGCAAGTCCATAGGAGG+AGG + Chr7:257134-257153 MsG0780035932.01.T01:CDS 50.0%
GAGAGATGCAAGTCCATAGG+AGG + Chr7:257131-257150 MsG0780035932.01.T01:CDS 50.0%
GATAACGAAGCTCCTTCCCA+AGG + Chr7:257458-257477 MsG0780035932.01.T01:CDS 50.0%
TGGGAGAGATGCAAGTCCAT+AGG + Chr7:257128-257147 MsG0780035932.01.T01:CDS 50.0%
! AATCATGGCCTAGTCTTCGC+GGG + Chr7:257219-257238 MsG0780035932.01.T01:CDS 50.0%
AAGTCCATAGGAGGAGGAGG+AGG + Chr7:257140-257159 MsG0780035932.01.T01:CDS 55.0%
ACAAAGAAAGTGGCCCCGGA+GGG + Chr7:257255-257274 MsG0780035932.01.T01:CDS 55.0%
CACCGAAAAACATCCCTCCG+GGG - Chr7:257271-257290 None:intergenic 55.0%
TGCAAGTCCATAGGAGGAGG+AGG + Chr7:257137-257156 MsG0780035932.01.T01:CDS 55.0%
! ATCATGGCCTAGTCTTCGCG+GGG + Chr7:257220-257239 MsG0780035932.01.T01:CDS 55.0%
GACAAAGAAAGTGGCCCCGG+AGG + Chr7:257254-257273 MsG0780035932.01.T01:CDS 60.0%
TCCATAGGAGGAGGAGGAGG+AGG + Chr7:257143-257162 MsG0780035932.01.T01:CDS 60.0%
TCCTCCTCCTCCTCCTCCTA+TGG - Chr7:257147-257166 None:intergenic 60.0%
TTCTTCGCCCCGCGAAGACT+AGG - Chr7:257230-257249 None:intergenic 60.0%
! GGCCCCGGAGGGATGTTTTT+CGG + Chr7:257266-257285 MsG0780035932.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 257062 257556 257062 ID=MsG0780035932.01;Name=MsG0780035932.01
Chr7 mRNA 257062 257556 257062 ID=MsG0780035932.01.T01;Parent=MsG0780035932.01;Name=MsG0780035932.01.T01;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|164
Chr7 exon 257062 257556 257062 ID=MsG0780035932.01.T01:exon:1606;Parent=MsG0780035932.01.T01
Chr7 CDS 257062 257556 257062 ID=MsG0780035932.01.T01:cds;Parent=MsG0780035932.01.T01
Gene Sequence

>MsG0780035932.01.T01

ATGGATATTGGCAAGAACATGAAGCAAGGAAAAGGGAACAAGAAAATAGGCCTCATAACAAAAACGTGGGAGAGATGCAAGTCCATAGGAGGAGGAGGAGGAGGACACAAGAGTAAATCATATTCATCATCAGTGATTCCAACGACAAGAAGGAGCAAATCATGGCCTAGTCTTCGCGGGGCGAAGAAAATAGACAAAGAAAGTGGCCCCGGAGGGATGTTTTTCGGTGTACGTTGGACCTCAAATGCAAAGGTTTGTGGTCAAACTGAGTATGCTAATCATCCTTTGTTCAAGATGTTGCTTGAAGAAGCTGAATCAGAATATGGTTATAGCTGCCAAGGACCTTTGGCTCTTCCTTGTAATGTTGATGTCTTTTACAAAGTGTTGATGGAAATGGATAACGAAGCTCCTTCCCAAGGTTGTACTTTTGGTCGTTCTCGTTCTTCTTATCATCTTCTTAGCCCTTCCAGGATGATTGTCTTAAACAACTTCTAA

Protein sequence

>MsG0780035932.01.T01

MDIGKNMKQGKGNKKIGLITKTWERCKSIGGGGGGHKSKSYSSSVIPTTRRSKSWPSLRGAKKIDKESGPGGMFFGVRWTSNAKVCGQTEYANHPLFKMLLEEAESEYGYSCQGPLALPCNVDVFYKVLMEMDNEAPSQGCTFGRSRSSYHLLSPSRMIVLNNF*