AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019590.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019590.01.T01 MTR_4g035310 67.213 122 25 1 1 122 1 107 3.25e-51 163
MsG0480019590.01.T01 MTR_4g035310 67.213 122 25 1 1 122 1 107 3.38e-51 163
MsG0480019590.01.T01 MTR_3g116130 49.057 106 39 1 10 115 23 113 4.88e-28 103
MsG0480019590.01.T01 MTR_3g116130 49.057 106 39 1 10 115 23 113 6.32e-27 102
MsG0480019590.01.T01 MTR_8g024520 44.444 117 50 2 5 121 14 115 1.86e-26 100
MsG0480019590.01.T01 MTR_8g024520 44.444 117 50 2 5 121 14 115 1.92e-26 100
MsG0480019590.01.T01 MTR_5g064120 45.000 100 40 1 9 108 9 93 6.46e-23 90.9
MsG0480019590.01.T01 MTR_3g092490 39.623 106 46 2 11 116 21 108 3.72e-20 81.3
MsG0480019590.01.T01 MTR_7g117495 41.346 104 46 1 9 112 11 99 9.24e-20 82.0
MsG0480019590.01.T01 MTR_7g117495 41.346 104 46 1 9 112 11 99 1.10e-19 81.6
MsG0480019590.01.T01 MTR_3g092490 39.623 106 46 2 11 116 21 108 1.21e-19 81.6
MsG0480019590.01.T01 MTR_3g092490 39.623 106 46 2 11 116 21 108 1.27e-19 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019590.01.T01 AT1G22910 55.357 112 35 1 11 122 8 104 5.54e-35 121
MsG0480019590.01.T01 AT1G22910 55.357 112 35 1 11 122 8 104 9.36e-35 120
MsG0480019590.01.T01 AT1G22910 55.357 112 35 1 11 122 8 104 1.04e-33 120
MsG0480019590.01.T01 AT1G33470 46.903 113 45 1 12 124 3 100 6.48e-30 108
MsG0480019590.01.T01 AT1G33470 46.903 113 45 1 12 124 3 100 7.23e-30 108
MsG0480019590.01.T01 AT1G33470 46.903 113 45 1 12 124 3 100 7.32e-30 108
MsG0480019590.01.T01 AT1G20880 50.000 106 36 2 10 115 18 106 3.03e-27 102
MsG0480019590.01.T01 AT1G20880 50.000 106 36 2 10 115 18 106 3.03e-27 102
MsG0480019590.01.T01 AT1G20880 50.000 106 36 2 10 115 18 106 3.03e-27 102
MsG0480019590.01.T01 AT1G76460 47.706 109 40 2 10 118 18 109 2.13e-26 100
MsG0480019590.01.T01 AT2G46780 44.144 111 46 2 12 121 18 113 5.93e-25 97.1
MsG0480019590.01.T01 AT2G46780 44.144 111 46 2 12 121 18 113 5.93e-25 97.1
MsG0480019590.01.T01 AT1G22330 42.727 110 46 2 10 119 11 103 5.41e-23 91.3
MsG0480019590.01.T01 AT1G78260 43.434 99 41 1 10 108 11 94 2.69e-21 86.3
MsG0480019590.01.T01 AT1G78260 43.434 99 41 1 10 108 11 94 3.26e-21 86.3
MsG0480019590.01.T01 AT3G54770 41.600 125 51 2 5 122 6 115 4.24e-21 85.5
MsG0480019590.01.T01 AT3G54770 41.600 125 51 2 5 122 6 115 4.78e-21 85.5
MsG0480019590.01.T01 AT1G22910 82.222 45 8 0 77 121 8 52 8.53e-21 83.6

Find 23 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGTCCTGCCAAATTAAT+TGG 0.140954 4:+22855718 None:intergenic
CAACATGACTCCAATTAATT+TGG 0.208507 4:-22855728 None:intergenic
TAACTGCAACCTTGCTTCTT+TGG 0.220641 4:-22855069 MsG0480019590.01.T01:CDS
ATTAATTTGGCAGGACAATT+TGG 0.230601 4:-22855715 MsG0480019590.01.T01:CDS
AACTGCAACCTTGCTTCTTT+GGG 0.243448 4:-22855068 MsG0480019590.01.T01:CDS
GAAGAAGTATTTGAGCAATT+TGG 0.290026 4:-22855629 MsG0480019590.01.T01:CDS
GTGTTGAAGGCTTAGATCTT+TGG 0.295951 4:+22855042 None:intergenic
GCTCTCCTACCATCAATTAT+AGG 0.326846 4:+22855092 None:intergenic
ATATTAATATTACCATGTTT+TGG 0.333370 4:+22855020 None:intergenic
ATTGCTCAAATACTTCTTCA+TGG 0.339874 4:+22855633 None:intergenic
TGAGCAATTTGGTGAGATCT+TGG 0.411455 4:-22855618 MsG0480019590.01.T01:CDS
ACATACAAATCCATAGCCTT+TGG 0.439269 4:+22855559 None:intergenic
ATGACTCCAATTAATTTGGC+AGG 0.491636 4:-22855724 MsG0480019590.01.T01:CDS
CAAGCTCTCATAGCAGCATC+AGG 0.508307 4:+22855128 None:intergenic
GATGCTGCTCCTATAATTGA+TGG 0.515656 4:-22855101 MsG0480019590.01.T01:CDS
ACTGGAAGATCCAAAGGCTA+TGG 0.530867 4:-22855569 MsG0480019590.01.T01:CDS
ATAGCCTTTGGATCTTCCAG+TGG 0.554952 4:+22855571 None:intergenic
TGCTGCTATGAGAGCTTGTG+TGG 0.555924 4:-22855123 MsG0480019590.01.T01:CDS
CTGCTCCTATAATTGATGGT+AGG 0.595874 4:-22855097 MsG0480019590.01.T01:CDS
GTTATCACTGACAAAGCCAC+TGG 0.609344 4:-22855587 MsG0480019590.01.T01:CDS
TTTGGACACCCAAAGAAGCA+AGG 0.627687 4:+22855060 None:intergenic
AAAGCCACTGGAAGATCCAA+AGG 0.627799 4:-22855575 MsG0480019590.01.T01:CDS
AAGCCTTCAACACCAAAACA+TGG 0.645444 4:-22855032 MsG0480019590.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATAAAAAAAATACAAA+TGG + Chr4:22855520-22855539 None:intergenic 10.0%
!!! CATTTGTATTTTTTTTATTT+TGG - Chr4:22855518-22855537 MsG0480019590.01.T01:intron 10.0%
!! TAATTTATTATTCAAGTAGA+AGG - Chr4:22855263-22855282 MsG0480019590.01.T01:intron 15.0%
!!! ATATTAATATTACCATGTTT+TGG + Chr4:22855720-22855739 None:intergenic 15.0%
!!! AATTTGTTTGTTTCCAATTT+AGG - Chr4:22855572-22855591 MsG0480019590.01.T01:CDS 20.0%
AATTGTCCTGCCAAATTAAT+TGG + Chr4:22855022-22855041 None:intergenic 30.0%
ATTGCTCAAATACTTCTTCA+TGG + Chr4:22855107-22855126 None:intergenic 30.0%
CTAGTGAAAGAAACTAAACA+TGG + Chr4:22855433-22855452 None:intergenic 30.0%
TAATCCTCCAACAAAAACTT+TGG + Chr4:22855062-22855081 None:intergenic 30.0%
! ACATACACCAAAGTTTTTGT+TGG - Chr4:22855052-22855071 MsG0480019590.01.T01:CDS 30.0%
! ATTAATTTGGCAGGACAATT+TGG - Chr4:22855022-22855041 MsG0480019590.01.T01:CDS 30.0%
!! GAAGAAGTATTTGAGCAATT+TGG - Chr4:22855108-22855127 MsG0480019590.01.T01:CDS 30.0%
ACATACAAATCCATAGCCTT+TGG + Chr4:22855181-22855200 None:intergenic 35.0%
GATTGAATCTCAATGAATCG+TGG - Chr4:22855358-22855377 MsG0480019590.01.T01:intron 35.0%
TCACGAAAAGTGACCTAAAT+TGG + Chr4:22855588-22855607 None:intergenic 35.0%
! ATGACTCCAATTAATTTGGC+AGG - Chr4:22855013-22855032 MsG0480019590.01.T01:CDS 35.0%
! TACACCAAAGTTTTTGTTGG+AGG - Chr4:22855055-22855074 MsG0480019590.01.T01:CDS 35.0%
!!! TTACCATGTTTTGGTGTTGA+AGG + Chr4:22855711-22855730 None:intergenic 35.0%
!!! TTTTGTTGGAGGATTAGCTT+GGG - Chr4:22855066-22855085 MsG0480019590.01.T01:CDS 35.0%
!!! TTTTTGTTGGAGGATTAGCT+TGG - Chr4:22855065-22855084 MsG0480019590.01.T01:CDS 35.0%
AACTGCAACCTTGCTTCTTT+GGG - Chr4:22855669-22855688 MsG0480019590.01.T01:CDS 40.0%
AAGCCTTCAACACCAAAACA+TGG - Chr4:22855705-22855724 MsG0480019590.01.T01:CDS 40.0%
GATGCTGCTCCTATAATTGA+TGG - Chr4:22855636-22855655 MsG0480019590.01.T01:CDS 40.0%
GCTCTCCTACCATCAATTAT+AGG + Chr4:22855648-22855667 None:intergenic 40.0%
GTGTTGAAGGCTTAGATCTT+TGG + Chr4:22855698-22855717 None:intergenic 40.0%
TAACTGCAACCTTGCTTCTT+TGG - Chr4:22855668-22855687 MsG0480019590.01.T01:CDS 40.0%
! CTGCTCCTATAATTGATGGT+AGG - Chr4:22855640-22855659 MsG0480019590.01.T01:CDS 40.0%
! TGAGCAATTTGGTGAGATCT+TGG - Chr4:22855119-22855138 MsG0480019590.01.T01:CDS 40.0%
AAAGCCACTGGAAGATCCAA+AGG - Chr4:22855162-22855181 MsG0480019590.01.T01:intron 45.0%
ACTGGAAGATCCAAAGGCTA+TGG - Chr4:22855168-22855187 MsG0480019590.01.T01:intron 45.0%
ATAGCCTTTGGATCTTCCAG+TGG + Chr4:22855169-22855188 None:intergenic 45.0%
GTTATCACTGACAAAGCCAC+TGG - Chr4:22855150-22855169 MsG0480019590.01.T01:intron 45.0%
TTTGGACACCCAAAGAAGCA+AGG + Chr4:22855680-22855699 None:intergenic 45.0%
CAAGCTCTCATAGCAGCATC+AGG + Chr4:22855612-22855631 None:intergenic 50.0%
TGCTGCTATGAGAGCTTGTG+TGG - Chr4:22855614-22855633 MsG0480019590.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 22855013 22855746 22855013 ID=MsG0480019590.01;Name=MsG0480019590.01
Chr4 mRNA 22855013 22855746 22855013 ID=MsG0480019590.01.T01;Parent=MsG0480019590.01;Name=MsG0480019590.01.T01;_AED=0.48;_eAED=0.63;_QI=0|0|0|1|0|0.5|2|0|126
Chr4 exon 22855519 22855746 22855519 ID=MsG0480019590.01.T01:exon:10743;Parent=MsG0480019590.01.T01
Chr4 exon 22855013 22855165 22855013 ID=MsG0480019590.01.T01:exon:10742;Parent=MsG0480019590.01.T01
Chr4 CDS 22855519 22855746 22855519 ID=MsG0480019590.01.T01:cds;Parent=MsG0480019590.01.T01
Chr4 CDS 22855013 22855165 22855013 ID=MsG0480019590.01.T01:cds;Parent=MsG0480019590.01.T01
Gene Sequence

>MsG0480019590.01.T01

ATGACTCCAATTAATTTGGCAGGACAATTTGGTGACACAACATACACCAAAGTTTTTGTTGGAGGATTAGCTTGGGAAACTCAAAAAGAGACCATGAAGAAGTATTTGAGCAATTTGGTGAGATCTTGGAAGCTGTTGTTATCACTGACAAAGCCACTGGAAGATCCAAAGGCTATGGATTTGTATGTCTATCTTCTCTCTATATTTGAATTTTTTTATCATTATCATGTCACTTTTCGTGAACCTGATGCTGCTATGAGAGCTTGTGTGGATGCTGCTCCTATAATTGATGGTAGGAGAGCTAACTGCAACCTTGCTTCTTTGGGTGTCCAAAGATCTAAGCCTTCAACACCAAAACATGGTAATATTAATATTGTATAA

Protein sequence

>MsG0480019590.01.T01

MTPINLAGQFGDTTYTKVFVGGLAWETQKETMKKYLSNLVRSWKLLLSLTKPLEDPKAMDLYVYLLSIFEFFYHYHVTFREPDAAMRACVDAAPIIDGRRANCNLASLGVQRSKPSTPKHGNINIV*