Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021883.01.T01 | XP_003607460.1 | 100 | 110 | 0 | 0 | 1 | 110 | 58 | 167 | 5.58E-70 | 216 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021883.01.T01 | B7FGK0 | 100.000 | 110 | 0 | 0 | 1 | 110 | 58 | 167 | 2.66e-70 | 216 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000157.01 | MsG0480021883.01 | 0.810647 | 1.038803e-50 | 6.296893e-48 |
| MsG0180000585.01 | MsG0480021883.01 | 0.800548 | 1.368924e-48 | 6.391805e-46 |
| MsG0180000669.01 | MsG0480021883.01 | 0.829962 | 3.838258e-55 | 3.988647e-52 |
| MsG0180000673.01 | MsG0480021883.01 | 0.828989 | 6.614864e-55 | 6.680641e-52 |
| MsG0180001043.01 | MsG0480021883.01 | 0.845460 | 4.004718e-59 | 6.678616e-56 |
| MsG0180001355.01 | MsG0480021883.01 | 0.802299 | 5.991532e-49 | 2.924746e-46 |
| MsG0180001921.01 | MsG0480021883.01 | 0.857794 | 1.280439e-62 | 3.197817e-59 |
| MsG0180002912.01 | MsG0480021883.01 | 0.820433 | 6.878617e-53 | 5.442095e-50 |
| MsG0180004005.01 | MsG0480021883.01 | 0.800388 | 1.475610e-48 | 6.862164e-46 |
| MsG0180004388.01 | MsG0480021883.01 | 0.816562 | 5.186844e-52 | 3.687796e-49 |
| MsG0180005125.01 | MsG0480021883.01 | 0.824790 | 6.668880e-54 | 5.967860e-51 |
| MsG0180005872.01 | MsG0480021883.01 | 0.807970 | 3.895835e-50 | 2.200230e-47 |
| MsG0180005878.01 | MsG0480021883.01 | 0.815596 | 8.525396e-52 | 5.903552e-49 |
| MsG0180006016.01 | MsG0480021883.01 | 0.840785 | 7.055334e-58 | 1.015866e-54 |
| MsG0180006069.01 | MsG0480021883.01 | 0.804671 | 1.931589e-49 | 1.001941e-46 |
| MsG0280010739.01 | MsG0480021883.01 | 0.819652 | 1.038086e-52 | 8.036126e-50 |
| MsG0280011141.01 | MsG0480021883.01 | 0.813759 | 2.175774e-51 | 1.433247e-48 |
| MsG0280011235.01 | MsG0480021883.01 | 0.802019 | 6.842810e-49 | 3.316321e-46 |
| MsG0280011446.01 | MsG0480021883.01 | 0.841394 | 4.881264e-58 | 7.162769e-55 |
| MsG0380011754.01 | MsG0480021883.01 | 0.825451 | 4.653716e-54 | 4.243107e-51 |
| MsG0380013047.01 | MsG0480021883.01 | 0.804453 | 2.144453e-49 | 1.106097e-46 |
| MsG0380014680.01 | MsG0480021883.01 | 0.846541 | 2.036016e-59 | 3.516186e-56 |
| MsG0480018970.01 | MsG0480021883.01 | 0.823070 | 1.688169e-53 | 1.438628e-50 |
| MsG0480020445.01 | MsG0480021883.01 | 0.840486 | 8.446425e-58 | 1.204954e-54 |
| MsG0480021731.01 | MsG0480021883.01 | 0.833179 | 6.188468e-56 | 7.071823e-53 |
| MsG0480021883.01 | MsG0480021953.01 | 0.820854 | 5.504730e-53 | 4.406420e-50 |
| MsG0480021883.01 | MsG0480022274.01 | 0.829061 | 6.354024e-55 | 6.431079e-52 |
| MsG0480021883.01 | MsG0480022480.01 | 0.825219 | 5.281857e-54 | 4.784607e-51 |
| MsG0480021883.01 | MsG0480023088.01 | 0.807203 | 5.668256e-50 | 3.137939e-47 |
| MsG0480021883.01 | MsG0480023517.01 | 0.838399 | 2.942311e-57 | 3.936280e-54 |
| MsG0480021883.01 | MsG0580024073.01 | 0.800906 | 1.156850e-48 | 5.451207e-46 |
| MsG0480021883.01 | MsG0580024114.01 | 0.811605 | 6.440345e-51 | 4.004005e-48 |
| MsG0480021883.01 | MsG0580024458.01 | -0.830258 | 3.249022e-55 | 3.405486e-52 |
| MsG0480021883.01 | MsG0580025134.01 | 0.825682 | 4.103738e-54 | 3.766605e-51 |
| MsG0480021883.01 | MsG0580025143.01 | 0.802669 | 5.026382e-49 | 2.476687e-46 |
| MsG0480021883.01 | MsG0580025328.01 | 0.807958 | 3.919931e-50 | 2.213165e-47 |
| MsG0480021883.01 | MsG0580025421.01 | 0.802931 | 4.437696e-49 | 2.201256e-46 |
| MsG0480021883.01 | MsG0580027034.01 | 0.806596 | 7.617640e-50 | 4.151267e-47 |
| MsG0480021883.01 | MsG0580027129.01 | 0.806684 | 7.298317e-50 | 3.986192e-47 |
| MsG0480021883.01 | MsG0580027345.01 | 0.853346 | 2.538777e-61 | 5.468831e-58 |
| MsG0480021883.01 | MsG0580029594.01 | 0.801572 | 8.451140e-49 | 4.049586e-46 |
| MsG0480021883.01 | MsG0580029758.01 | 0.821394 | 4.131991e-53 | 3.357599e-50 |
| MsG0480021883.01 | MsG0580029762.01 | 0.804413 | 2.186596e-49 | 1.126651e-46 |
| MsG0480021883.01 | MsG0580030235.01 | 0.825287 | 5.089808e-54 | 4.619498e-51 |
| MsG0480021883.01 | MsG0680031243.01 | 0.801605 | 8.319733e-49 | 3.989870e-46 |
| MsG0480021883.01 | MsG0680031538.01 | 0.808920 | 2.443197e-50 | 1.414678e-47 |
| MsG0480021883.01 | MsG0680031651.01 | 0.813805 | 2.124949e-51 | 1.401590e-48 |
| MsG0480021883.01 | MsG0680032430.01 | 0.811746 | 6.001039e-51 | 3.744962e-48 |
| MsG0480021883.01 | MsG0680033391.01 | 0.815870 | 7.405741e-52 | 5.166575e-49 |
| MsG0480021883.01 | MsG0680034450.01 | 0.816016 | 6.870232e-52 | 4.812498e-49 |
| MsG0480021883.01 | MsG0680035582.01 | 0.835765 | 1.387200e-56 | 1.713023e-53 |
| MsG0480021883.01 | MsG0780037109.01 | 0.819128 | 1.366301e-52 | 1.042385e-49 |
| MsG0480021883.01 | MsG0780037512.01 | 0.802603 | 5.186767e-49 | 2.551443e-46 |
| MsG0480021883.01 | MsG0780038495.01 | 0.837590 | 4.750785e-57 | 6.200341e-54 |
| MsG0480021883.01 | MsG0780038645.01 | 0.819221 | 1.301583e-52 | 9.955414e-50 |
| MsG0480021883.01 | MsG0780039841.01 | 0.803821 | 2.902717e-49 | 1.473138e-46 |
| MsG0480021883.01 | MsG0780040442.01 | 0.813491 | 2.491802e-51 | 1.629697e-48 |
| MsG0480021883.01 | MsG0780040726.01 | 0.814932 | 1.197451e-51 | 8.142279e-49 |
| MsG0480021883.01 | MsG0780040861.01 | 0.840400 | 8.897288e-58 | 1.265908e-54 |
| MsG0480021883.01 | MsG0780041161.01 | 0.800284 | 1.548816e-48 | 7.183876e-46 |
| MsG0480021883.01 | MsG0780041357.01 | 0.800105 | 1.684862e-48 | 7.779030e-46 |
| MsG0480021883.01 | MsG0780041399.01 | 0.810351 | 1.203644e-50 | 7.238634e-48 |
| MsG0480021883.01 | MsG0880041985.01 | 0.801080 | 1.066062e-48 | 5.045509e-46 |
| MsG0480021883.01 | MsG0880042294.01 | 0.808845 | 2.535486e-50 | 1.465205e-47 |
| MsG0480021883.01 | MsG0880042957.01 | 0.805766 | 1.139400e-49 | 6.079313e-47 |
| MsG0480021883.01 | MsG0880043002.01 | 0.818341 | 2.061874e-52 | 1.539359e-49 |
| MsG0480021883.01 | MsG0880043003.01 | 0.808287 | 3.335625e-50 | 1.899750e-47 |
| MsG0480021883.01 | MsG0880043082.01 | 0.821470 | 3.968618e-53 | 3.231515e-50 |
| MsG0480021883.01 | MsG0880043163.01 | 0.802571 | 5.266482e-49 | 2.588457e-46 |
| MsG0480021883.01 | MsG0880044062.01 | -0.821991 | 3.008313e-53 | 2.485905e-50 |
| MsG0480021883.01 | MsG0880045106.01 | 0.808446 | 3.085590e-50 | 1.764694e-47 |
| MsG0480021883.01 | MsG0880046058.01 | 0.820494 | 6.657365e-53 | 5.275830e-50 |
| MsG0480021883.01 | MsG0880046244.01 | 0.800426 | 1.449263e-48 | 6.746342e-46 |
| MsG0480021883.01 | MsG0880046285.01 | 0.806929 | 6.479900e-50 | 3.561729e-47 |
| MsG0480021883.01 | MsG0880046328.01 | 0.826368 | 2.819454e-54 | 2.638934e-51 |
| MsG0480021883.01 | MsG0880047116.01 | 0.804180 | 2.444378e-49 | 1.251997e-46 |
| MsG0480021883.01 | MsG0880047143.01 | 0.807979 | 3.879156e-50 | 2.191331e-47 |
| MsG0380015779.01 | MsG0480021883.01 | 0.845530 | 3.834456e-59 | 6.409009e-56 |
| MsG0380017226.01 | MsG0480021883.01 | 0.804845 | 1.776747e-49 | 9.258207e-47 |
| MsG0380017286.01 | MsG0480021883.01 | 0.856845 | 2.442953e-62 | 5.908527e-59 |
| MsG0380017403.01 | MsG0480021883.01 | 0.800512 | 1.392182e-48 | 6.494424e-46 |
| MsG0380018009.01 | MsG0480021883.01 | 0.836517 | 8.936395e-57 | 1.128763e-53 |
| MsG0280006595.01 | MsG0480021883.01 | 0.810094 | 1.367424e-50 | 8.168524e-48 |
| MsG0280006708.01 | MsG0480021883.01 | 0.838106 | 3.500886e-57 | 4.641553e-54 |
| MsG0280007088.01 | MsG0480021883.01 | 0.805393 | 1.364453e-49 | 7.210175e-47 |
| MsG0280007585.01 | MsG0480021883.01 | 0.810882 | 9.241730e-51 | 5.637498e-48 |
| MsG0280007609.01 | MsG0480021883.01 | 0.814945 | 1.189502e-51 | 8.091100e-49 |
| MsG0280008907.01 | MsG0480021883.01 | 0.800623 | 1.321598e-48 | 6.182581e-46 |
| MsG0280008971.01 | MsG0480021883.01 | 0.828480 | 8.783109e-55 | 8.740968e-52 |
| MsG0280009235.01 | MsG0480021883.01 | 0.839279 | 1.742698e-57 | 2.394828e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021883.01.T01 | MTR_4g078240 | 100.000 | 110 | 0 | 0 | 1 | 110 | 58 | 167 | 6.75e-74 | 216 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021883.01.T01 | AT4G03600 | 64.912 | 114 | 35 | 1 | 2 | 110 | 47 | 160 | 6.10e-47 | 148 |
| MsG0480021883.01.T01 | AT1G03730 | 67.857 | 112 | 34 | 1 | 1 | 110 | 54 | 165 | 1.03e-37 | 125 |
| MsG0480021883.01.T01 | AT4G28330 | 58.716 | 109 | 44 | 1 | 1 | 109 | 48 | 155 | 1.54e-32 | 111 |
| MsG0480021883.01.T01 | AT4G28340 | 59.615 | 104 | 41 | 1 | 1 | 104 | 48 | 150 | 1.67e-30 | 106 |
Find 21 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTGCTGTTTGTGCTGTTT+TGG | 0.192732 | 4:-65549336 | MsG0480021883.01.T01:CDS |
| TTCTCAAATCAATGCTTTCT+TGG | 0.227365 | 4:-65549622 | None:intergenic |
| ATCTATGCATCTGTTCTTCT+TGG | 0.241680 | 4:-65549557 | MsG0480021883.01.T01:CDS |
| GGTTGCTGCTTTGGTTATTA+TGG | 0.248141 | 4:-65549510 | MsG0480021883.01.T01:CDS |
| GCTTTCAATGGTTGCTGCTT+TGG | 0.275259 | 4:-65549519 | MsG0480021883.01.T01:CDS |
| AGAACAGATGCATAGATTGC+AGG | 0.399836 | 4:+65549563 | None:intergenic |
| GTTGAAGGAAGGAAGATTAC+TGG | 0.414092 | 4:-65549410 | MsG0480021883.01.T01:CDS |
| TATTATGGTTCCTGCAGTTA+TGG | 0.418968 | 4:-65549495 | MsG0480021883.01.T01:CDS |
| AGGAGGACTTTGGTGGTTGA+AGG | 0.432920 | 4:-65549425 | MsG0480021883.01.T01:CDS |
| GAAAGCCAACAAAACAACTA+TGG | 0.434853 | 4:+65549460 | None:intergenic |
| GGACTTTGGTGGTTGAAGGA+AGG | 0.465297 | 4:-65549421 | MsG0480021883.01.T01:CDS |
| TGGAAAACCTAGGAGGACTT+TGG | 0.466296 | 4:-65549435 | MsG0480021883.01.T01:CDS |
| CAAGCTCAATCATCAACCTC+TGG | 0.488041 | 4:-65549590 | MsG0480021883.01.T01:CDS |
| TCAACCACCAAAGTCCTCCT+AGG | 0.505379 | 4:+65549428 | None:intergenic |
| AAAACCTAGGAGGACTTTGG+TGG | 0.554975 | 4:-65549432 | MsG0480021883.01.T01:CDS |
| CTCAATCATCAACCTCTGGT+TGG | 0.564957 | 4:-65549586 | MsG0480021883.01.T01:CDS |
| ATCCACGTCACCATAACTGC+AGG | 0.577508 | 4:+65549485 | None:intergenic |
| TGGCTTTCTTTGGAAAACCT+AGG | 0.614419 | 4:-65549445 | MsG0480021883.01.T01:CDS |
| CTTTCTTTGGAAAACCTAGG+AGG | 0.626529 | 4:-65549442 | MsG0480021883.01.T01:CDS |
| ATAGATTGCAGGCCAACCAG+AGG | 0.699263 | 4:+65549574 | None:intergenic |
| TTCCTGCAGTTATGGTGACG+TGG | 0.716496 | 4:-65549487 | MsG0480021883.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAGATTTTGCTGAAAGA+AGG | - | Chr4:65549544-65549563 | MsG0480021883.01.T01:CDS | 25.0% |
| !! | TTAAGATTTTGCTGAAAGAA+GGG | - | Chr4:65549545-65549564 | MsG0480021883.01.T01:CDS | 25.0% |
| !! | GATAACCATAGTTGTTTTGT+TGG | - | Chr4:65549447-65549466 | MsG0480021883.01.T01:CDS | 30.0% |
| !! | TTCTTCTTGGAACTGTTTTT+GGG | - | Chr4:65549368-65549387 | MsG0480021883.01.T01:CDS | 30.0% |
| !!! | GGTATTTTGTTTTGGTTAGA+AGG | - | Chr4:65549599-65549618 | MsG0480021883.01.T01:CDS | 30.0% |
| !!! | TGTTTTGGGGTATTTTGTTT+TGG | - | Chr4:65549591-65549610 | MsG0480021883.01.T01:CDS | 30.0% |
| ATCTATGCATCTGTTCTTCT+TGG | - | Chr4:65549355-65549374 | MsG0480021883.01.T01:CDS | 35.0% | |
| GAAAGCCAACAAAACAACTA+TGG | + | Chr4:65549455-65549474 | None:intergenic | 35.0% | |
| TATTATGGTTCCTGCAGTTA+TGG | - | Chr4:65549417-65549436 | MsG0480021883.01.T01:CDS | 35.0% | |
| ! | GTTCTTCTTGGAACTGTTTT+TGG | - | Chr4:65549367-65549386 | MsG0480021883.01.T01:CDS | 35.0% |
| !!! | GTTGTTTTGTTGGCTTTCTT+TGG | - | Chr4:65549457-65549476 | MsG0480021883.01.T01:CDS | 35.0% |
| !!! | TGTTTTTGGGTTGCTTTCAA+TGG | - | Chr4:65549381-65549400 | MsG0480021883.01.T01:CDS | 35.0% |
| AGAACAGATGCATAGATTGC+AGG | + | Chr4:65549352-65549371 | None:intergenic | 40.0% | |
| GTTGAAGGAAGGAAGATTAC+TGG | - | Chr4:65549502-65549521 | MsG0480021883.01.T01:CDS | 40.0% | |
| ! | CTTTCTTTGGAAAACCTAGG+AGG | - | Chr4:65549470-65549489 | MsG0480021883.01.T01:CDS | 40.0% |
| ! | TGGCTTTCTTTGGAAAACCT+AGG | - | Chr4:65549467-65549486 | MsG0480021883.01.T01:CDS | 40.0% |
| !! | GGTTGCTGCTTTGGTTATTA+TGG | - | Chr4:65549402-65549421 | MsG0480021883.01.T01:CDS | 40.0% |
| AAAACCTAGGAGGACTTTGG+TGG | - | Chr4:65549480-65549499 | MsG0480021883.01.T01:CDS | 45.0% | |
| CAAGCTCAATCATCAACCTC+TGG | - | Chr4:65549322-65549341 | MsG0480021883.01.T01:CDS | 45.0% | |
| CTCAATCATCAACCTCTGGT+TGG | - | Chr4:65549326-65549345 | MsG0480021883.01.T01:CDS | 45.0% | |
| TGGAAAACCTAGGAGGACTT+TGG | - | Chr4:65549477-65549496 | MsG0480021883.01.T01:CDS | 45.0% | |
| ! | GCTTTCAATGGTTGCTGCTT+TGG | - | Chr4:65549393-65549412 | MsG0480021883.01.T01:CDS | 45.0% |
| !!! | CTGCTGTTTGTGCTGTTTTG+GGG | - | Chr4:65549578-65549597 | MsG0480021883.01.T01:CDS | 45.0% |
| !!! | GCTGCTGTTTGTGCTGTTTT+GGG | - | Chr4:65549577-65549596 | MsG0480021883.01.T01:CDS | 45.0% |
| !!! | TGCTGCTGTTTGTGCTGTTT+TGG | - | Chr4:65549576-65549595 | MsG0480021883.01.T01:CDS | 45.0% |
| ATAGATTGCAGGCCAACCAG+AGG | + | Chr4:65549341-65549360 | None:intergenic | 50.0% | |
| ATCCACGTCACCATAACTGC+AGG | + | Chr4:65549430-65549449 | None:intergenic | 50.0% | |
| TCAACCACCAAAGTCCTCCT+AGG | + | Chr4:65549487-65549506 | None:intergenic | 50.0% | |
| ! | AGGAGGACTTTGGTGGTTGA+AGG | - | Chr4:65549487-65549506 | MsG0480021883.01.T01:CDS | 50.0% |
| ! | GGACTTTGGTGGTTGAAGGA+AGG | - | Chr4:65549491-65549510 | MsG0480021883.01.T01:CDS | 50.0% |
| ! | TTCCTGCAGTTATGGTGACG+TGG | - | Chr4:65549425-65549444 | MsG0480021883.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 65549301 | 65549633 | 65549301 | ID=MsG0480021883.01;Name=MsG0480021883.01 |
| Chr4 | mRNA | 65549301 | 65549633 | 65549301 | ID=MsG0480021883.01.T01;Parent=MsG0480021883.01;Name=MsG0480021883.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|110 |
| Chr4 | exon | 65549301 | 65549633 | 65549301 | ID=MsG0480021883.01.T01:exon:7967;Parent=MsG0480021883.01.T01 |
| Chr4 | CDS | 65549301 | 65549633 | 65549301 | ID=MsG0480021883.01.T01:cds;Parent=MsG0480021883.01.T01 |
| Gene Sequence |
| Protein sequence |
