AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043208.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043208.01.T01 MTR_8g031470 95.876 194 8 0 9 202 305 498 5.36e-138 395
MsG0880043208.01.T01 MTR_8g031450 82.474 194 32 2 9 202 324 515 8.11e-115 337
MsG0880043208.01.T01 MTR_8g031470 82.199 191 23 2 13 202 281 461 4.57e-107 315
MsG0880043208.01.T01 MTR_5g010500 61.376 189 72 1 9 197 313 500 2.70e-81 250
MsG0880043208.01.T01 MTR_0014s0440 59.686 191 75 2 9 199 306 494 1.66e-79 246
MsG0880043208.01.T01 MTR_0014s0440 59.686 191 75 2 9 199 317 505 2.73e-79 245
MsG0880043208.01.T01 MTR_8g031290 57.778 180 74 2 9 188 311 488 5.47e-69 218
MsG0880043208.01.T01 MTR_7g076860 58.750 160 64 1 9 168 239 396 1.31e-64 204
MsG0880043208.01.T01 MTR_7g076890 55.000 160 70 1 9 168 269 426 5.18e-61 196
MsG0880043208.01.T01 MTR_3g464000 44.304 158 84 3 9 166 312 465 3.73e-43 150
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043208.01.T01 AT2G45830 66.667 186 60 2 9 194 189 372 4.05e-87 261
MsG0880043208.01.T01 AT2G45830 66.667 186 60 2 9 194 189 372 4.05e-87 261
MsG0880043208.01.T01 AT2G45830 66.667 186 60 2 9 194 189 372 4.05e-87 261
MsG0880043208.01.T01 AT2G45830 66.667 186 60 2 9 194 189 372 4.05e-87 261
MsG0880043208.01.T01 AT2G45830 66.667 186 60 2 9 194 330 513 9.21e-86 263
MsG0880043208.01.T01 AT2G45830 66.667 186 60 2 9 194 330 513 9.21e-86 263
MsG0880043208.01.T01 AT3G61270 65.608 189 63 2 9 197 188 374 5.99e-85 256
MsG0880043208.01.T01 AT3G61270 65.608 189 63 2 9 197 307 493 7.34e-84 257
MsG0880043208.01.T01 AT5G23850 60.417 192 74 2 9 200 350 539 2.10e-81 252
MsG0880043208.01.T01 AT1G63420 60.417 192 71 2 9 196 385 575 1.53e-77 243
MsG0880043208.01.T01 AT3G48980 55.729 192 83 2 9 200 347 536 3.70e-76 238
MsG0880043208.01.T01 AT3G48980 55.729 192 83 2 9 200 353 542 3.99e-76 238
MsG0880043208.01.T01 AT2G45840 52.604 192 90 1 9 200 329 519 7.74e-69 219
MsG0880043208.01.T01 AT3G61290 51.562 192 93 0 9 200 258 449 1.68e-66 211
MsG0880043208.01.T01 AT3G61280 50.262 191 95 0 9 199 344 534 1.33e-64 208
MsG0880043208.01.T01 AT1G07220 45.355 183 95 4 9 191 321 498 3.84e-45 156
MsG0880043208.01.T01 AT1G63420 72.603 73 20 0 9 81 385 457 2.58e-31 119

Find 39 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATAATGCAGGTGGAAATT+TGG 0.133817 8:+20643837 MsG0880043208.01.T01:CDS
TAGAATGATACATGTGATTT+TGG 0.158379 8:+20643141 None:intergenic
TGGTGCCATTACAACATTAT+TGG 0.306283 8:+20643290 MsG0880043208.01.T01:CDS
AATAATGCAGGTGGAAATTT+GGG 0.316139 8:+20643838 MsG0880043208.01.T01:CDS
CATTTACTATTGTCTCTAAT+AGG 0.329746 8:-20643313 None:intergenic
TCTTAAATTTGCTGTGGAAT+GGG 0.339721 8:+20643342 MsG0880043208.01.T01:CDS
CTCTTAAATTTGCTGTGGAA+TGG 0.371549 8:+20643341 MsG0880043208.01.T01:CDS
TCTAAATCTAAAGGATCATA+TGG 0.408752 8:-20643788 None:intergenic
AAATTTGGGAAGATGAATAT+TGG 0.433602 8:+20643852 MsG0880043208.01.T01:CDS
ACAGCTCCTCTAGGTATAGT+TGG 0.445298 8:-20643647 None:intergenic
TGGTAACAAAAGGAGGTTCA+TGG 0.463575 8:+20643712 MsG0880043208.01.T01:CDS
TTAATGACATGTGATGTATT+AGG 0.469892 8:+20643689 MsG0880043208.01.T01:CDS
ATCTAACAGGCACAGTCAAT+AGG 0.470737 8:+20643521 MsG0880043208.01.T01:intron
ATAGGCCAATAATGTTGTAA+TGG 0.494794 8:-20643295 None:intergenic
ATGTATTAGGTGGTAACAAA+AGG 0.498930 8:+20643702 MsG0880043208.01.T01:CDS
GCACAGTCAATAGGAGAGGC+TGG 0.500817 8:+20643530 MsG0880043208.01.T01:CDS
AAAAGCTAATTCAATAATGC+AGG 0.515286 8:+20643826 MsG0880043208.01.T01:CDS
GAGGTTCATGGAAGAGTCAA+TGG 0.516306 8:+20643724 MsG0880043208.01.T01:CDS
CTTAAATTTGCTGTGGAATG+GGG 0.516885 8:+20643343 MsG0880043208.01.T01:CDS
AGGTACAAGATATACATAGA+AGG 0.526786 8:+20643172 MsG0880043208.01.T01:CDS
ACAAGATATACATAGAAGGA+TGG 0.528604 8:+20643176 MsG0880043208.01.T01:CDS
CAAATTTCCACAGCTCCTCT+AGG 0.531706 8:-20643656 None:intergenic
GGGCAACAATCACACTCATA+AGG 0.533532 8:+20643363 MsG0880043208.01.T01:CDS
CACCTCTCTTAAATTTGCTG+TGG 0.534214 8:+20643336 MsG0880043208.01.T01:CDS
TATACATAGAAGGATGGGCT+TGG 0.537356 8:+20643182 MsG0880043208.01.T01:CDS
CAAGATATACATAGAAGGAT+GGG 0.562972 8:+20643177 MsG0880043208.01.T01:CDS
AGCTAATTCAATAATGCAGG+TGG 0.565125 8:+20643829 MsG0880043208.01.T01:CDS
GGACTTTACATATAATGTCA+TGG 0.570834 8:-20643239 None:intergenic
AACTATACCTAGAGGAGCTG+TGG 0.578398 8:+20643649 MsG0880043208.01.T01:CDS
TCTAAAGGATCATATGGAGG+AGG 0.587652 8:-20643782 None:intergenic
CATGGAAGAGTCAATGGTGA+AGG 0.603386 8:+20643730 MsG0880043208.01.T01:CDS
TATGGAGGAGGAATAGTGCA+TGG 0.604369 8:-20643770 None:intergenic
TATTAGGTGGTAACAAAAGG+AGG 0.620574 8:+20643705 MsG0880043208.01.T01:CDS
TGGTAGCAAATTCATACAAG+AGG 0.633461 8:+20643550 MsG0880043208.01.T01:CDS
TTCCACAGCAAATTTAAGAG+AGG 0.634434 8:-20643338 None:intergenic
AAATCTAAAGGATCATATGG+AGG 0.667890 8:-20643785 None:intergenic
ACAGGCACAGTCAATAGGAG+AGG 0.670596 8:+20643526 MsG0880043208.01.T01:intron
TTCAAACCAACTATACCTAG+AGG 0.684725 8:+20643641 MsG0880043208.01.T01:CDS
ATGACATGTGATGTATTAGG+TGG 0.728891 8:+20643692 MsG0880043208.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTCTTCTAAATCTAA+AGG - Chr8:20643800-20643819 None:intergenic 15.0%
!! AATGTAACAAAAAAATTTCA+TGG - Chr8:20643479-20643498 None:intergenic 15.0%
!! TCAAAGTAAAATAAAATCAT+AGG - Chr8:20643435-20643454 None:intergenic 15.0%
!!! TAGATTTAGAAGAATTTTTA+GGG + Chr8:20643801-20643820 MsG0880043208.01.T01:CDS 15.0%
!!! TTAGATTTAGAAGAATTTTT+AGG + Chr8:20643800-20643819 MsG0880043208.01.T01:CDS 15.0%
!! AGTTAGTGAAAAATACATTA+TGG + Chr8:20643207-20643226 MsG0880043208.01.T01:CDS 20.0%
!! TATGAAAAAATCATGGTAAT+TGG - Chr8:20643263-20643282 None:intergenic 20.0%
!!! GATTTTATTTTACTTTGACT+TGG + Chr8:20643437-20643456 MsG0880043208.01.T01:intron 20.0%
! AAAAGCTAATTCAATAATGC+AGG + Chr8:20643826-20643845 MsG0880043208.01.T01:CDS 25.0%
! AAATTTGGGAAGATGAATAT+TGG + Chr8:20643852-20643871 MsG0880043208.01.T01:CDS 25.0%
! ATTCCCTTAAAATATCTAAC+AGG + Chr8:20643508-20643527 MsG0880043208.01.T01:intron 25.0%
! CATTTACTATTGTCTCTAAT+AGG - Chr8:20643316-20643335 None:intergenic 25.0%
! TCTAAATCTAAAGGATCATA+TGG - Chr8:20643791-20643810 None:intergenic 25.0%
! TTAATGACATGTGATGTATT+AGG + Chr8:20643689-20643708 MsG0880043208.01.T01:CDS 25.0%
!! TCACATGTCATTAATTTTTC+AGG - Chr8:20643683-20643702 None:intergenic 25.0%
!!! TACCATGATTTTTTCATAAG+AGG + Chr8:20643265-20643284 MsG0880043208.01.T01:CDS 25.0%
AAATCTAAAGGATCATATGG+AGG - Chr8:20643788-20643807 None:intergenic 30.0%
AATAATGCAGGTGGAAATTT+GGG + Chr8:20643838-20643857 MsG0880043208.01.T01:CDS 30.0%
ACAAGATATACATAGAAGGA+TGG + Chr8:20643176-20643195 MsG0880043208.01.T01:CDS 30.0%
AGGTACAAGATATACATAGA+AGG + Chr8:20643172-20643191 MsG0880043208.01.T01:CDS 30.0%
ATAGGCCAATAATGTTGTAA+TGG - Chr8:20643298-20643317 None:intergenic 30.0%
ATATTGGCAGAGTAAAAACA+AGG + Chr8:20643868-20643887 MsG0880043208.01.T01:CDS 30.0%
ATGTATTAGGTGGTAACAAA+AGG + Chr8:20643702-20643721 MsG0880043208.01.T01:CDS 30.0%
CAAGATATACATAGAAGGAT+GGG + Chr8:20643177-20643196 MsG0880043208.01.T01:CDS 30.0%
GGACTTTACATATAATGTCA+TGG - Chr8:20643242-20643261 None:intergenic 30.0%
TATTGGCAGAGTAAAAACAA+GGG + Chr8:20643869-20643888 MsG0880043208.01.T01:CDS 30.0%
TCTTGCTCATTTGAATAATG+TGG - Chr8:20643408-20643427 None:intergenic 30.0%
TGCCTCTTATGAAAAAATCA+TGG - Chr8:20643270-20643289 None:intergenic 30.0%
! TGTGCCTGTTAGATATTTTA+AGG - Chr8:20643515-20643534 None:intergenic 30.0%
!! GTGCCTGTTAGATATTTTAA+GGG - Chr8:20643514-20643533 None:intergenic 30.0%
!! TCTTAAATTTGCTGTGGAAT+GGG + Chr8:20643342-20643361 MsG0880043208.01.T01:CDS 30.0%
!!! TGATTTTTTCATAAGAGGCA+TGG + Chr8:20643270-20643289 MsG0880043208.01.T01:CDS 30.0%
AGCTAATTCAATAATGCAGG+TGG + Chr8:20643829-20643848 MsG0880043208.01.T01:CDS 35.0%
ATGACATGTGATGTATTAGG+TGG + Chr8:20643692-20643711 MsG0880043208.01.T01:CDS 35.0%
CAATAATGCAGGTGGAAATT+TGG + Chr8:20643837-20643856 MsG0880043208.01.T01:CDS 35.0%
TATTAGGTGGTAACAAAAGG+AGG + Chr8:20643705-20643724 MsG0880043208.01.T01:CDS 35.0%
TGGTAGCAAATTCATACAAG+AGG + Chr8:20643550-20643569 MsG0880043208.01.T01:CDS 35.0%
TTCAAACCAACTATACCTAG+AGG + Chr8:20643641-20643660 MsG0880043208.01.T01:CDS 35.0%
TTCCACAGCAAATTTAAGAG+AGG - Chr8:20643341-20643360 None:intergenic 35.0%
!! CTCTTAAATTTGCTGTGGAA+TGG + Chr8:20643341-20643360 MsG0880043208.01.T01:CDS 35.0%
!! CTTAAATTTGCTGTGGAATG+GGG + Chr8:20643343-20643362 MsG0880043208.01.T01:CDS 35.0%
!! TGGTGCCATTACAACATTAT+TGG + Chr8:20643290-20643309 MsG0880043208.01.T01:CDS 35.0%
!!! TGATACATGTGATTTTGGCT+TGG + Chr8:20643146-20643165 MsG0880043208.01.T01:CDS 35.0%
ATCTAACAGGCACAGTCAAT+AGG + Chr8:20643521-20643540 MsG0880043208.01.T01:intron 40.0%
TCTAAAGGATCATATGGAGG+AGG - Chr8:20643785-20643804 None:intergenic 40.0%
TGGTAACAAAAGGAGGTTCA+TGG + Chr8:20643712-20643731 MsG0880043208.01.T01:CDS 40.0%
! TATACATAGAAGGATGGGCT+TGG + Chr8:20643182-20643201 MsG0880043208.01.T01:CDS 40.0%
!! CACCTCTCTTAAATTTGCTG+TGG + Chr8:20643336-20643355 MsG0880043208.01.T01:CDS 40.0%
AACTATACCTAGAGGAGCTG+TGG + Chr8:20643649-20643668 MsG0880043208.01.T01:CDS 45.0%
ACAGCTCCTCTAGGTATAGT+TGG - Chr8:20643650-20643669 None:intergenic 45.0%
CAAATTTCCACAGCTCCTCT+AGG - Chr8:20643659-20643678 None:intergenic 45.0%
CATGGAAGAGTCAATGGTGA+AGG + Chr8:20643730-20643749 MsG0880043208.01.T01:CDS 45.0%
GAGGTTCATGGAAGAGTCAA+TGG + Chr8:20643724-20643743 MsG0880043208.01.T01:CDS 45.0%
GGGCAACAATCACACTCATA+AGG + Chr8:20643363-20643382 MsG0880043208.01.T01:CDS 45.0%
TATGGAGGAGGAATAGTGCA+TGG - Chr8:20643773-20643792 None:intergenic 45.0%
!!! ATGTGATTTTGGCTTGGCAC+AGG + Chr8:20643152-20643171 MsG0880043208.01.T01:CDS 45.0%
ACAGGCACAGTCAATAGGAG+AGG + Chr8:20643526-20643545 MsG0880043208.01.T01:intron 50.0%
GCACAGTCAATAGGAGAGGC+TGG + Chr8:20643530-20643549 MsG0880043208.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 20643145 20643905 20643145 ID=MsG0880043208.01;Name=MsG0880043208.01
Chr8 mRNA 20643145 20643905 20643145 ID=MsG0880043208.01.T01;Parent=MsG0880043208.01;Name=MsG0880043208.01.T01;_AED=0.49;_eAED=0.51;_QI=0|1|0.5|1|1|1|2|7|202
Chr8 exon 20643145 20643384 20643145 ID=MsG0880043208.01.T01:exon:5519;Parent=MsG0880043208.01.T01
Chr8 exon 20643530 20643905 20643530 ID=MsG0880043208.01.T01:exon:5520;Parent=MsG0880043208.01.T01
Chr8 CDS 20643145 20643384 20643145 ID=MsG0880043208.01.T01:cds;Parent=MsG0880043208.01.T01
Chr8 CDS 20643530 20643898 20643530 ID=MsG0880043208.01.T01:cds;Parent=MsG0880043208.01.T01
Chr8 three_prime_UTR 20643899 20643905 20643899 ID=MsG0880043208.01.T01:three_prime_utr;Parent=MsG0880043208.01.T01
Gene Sequence

>MsG0880043208.01.T01

ATGATACATGTGATTTTGGCTTGGCACAGGTACAAGATATACATAGAAGGATGGGCTTGGTCAGTTAGTGAAAAATACATTATGGCATGTGATTCCATGACATTATATGTAAAGTCCAATTACCATGATTTTTTCATAAGAGGCATGGTGCCATTACAACATTATTGGCCTATTAGAGACAATAGTAAATGCACCTCTCTTAAATTTGCTGTGGAATGGGGCAACAATCACACTCATAAGGCACAGTCAATAGGAGAGGCTGGTAGCAAATTCATACAAGAGGACTTAGACATGAAAAATGTATACAATTACATGTTTCATCTACTAAATGAATATGCAAAGCTTTTGAAGTTCAAACCAACTATACCTAGAGGAGCTGTGGAAATTTGTCCTGAAAAATTAATGACATGTGATGTATTAGGTGGTAACAAAAGGAGGTTCATGGAAGAGTCAATGGTGAAGGTTGCTAGTGATTCAAATCCATGCACTATTCCTCCTCCATATGATCCTTTAGATTTAGAAGAATTTTTAGGGAGAAAAGCTAATTCAATAATGCAGGTGGAAATTTGGGAAGATGAATATTGGCAGAGTAAAAACAAGGGACAATAG

Protein sequence

>MsG0880043208.01.T01

MIHVILAWHRYKIYIEGWAWSVSEKYIMACDSMTLYVKSNYHDFFIRGMVPLQHYWPIRDNSKCTSLKFAVEWGNNHTHKAQSIGEAGSKFIQEDLDMKNVYNYMFHLLNEYAKLLKFKPTIPRGAVEICPEKLMTCDVLGGNKRRFMEESMVKVASDSNPCTIPPPYDPLDLEEFLGRKANSIMQVEIWEDEYWQSKNKGQ*