AlfalfaGEDB | MsG0880044257.01

M. sativa cultivar ZhongmuNo.1 / MsG0880044257.01

KEGG: K11253
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044257.01.T01	RHN47114.1	58.407	113	18	3	249	334	556	666	1.67E-22	112

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044257.01.T01	P02302	91.429	35	3	0	332	366	54	88	1.99E-13	70.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044257.01.T01	A0A396H5W9	58.407	113	18	3	249	334	556	666	7.97e-23	112

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047797.01	MsG0880044257.01	0.822421	2.389976e-53	1.999554e-50
MsG0080048402.01	MsG0880044257.01	0.804476	2.121396e-49	1.094837e-46
MsG0080048874.01	MsG0880044257.01	0.802899	4.505650e-49	2.233238e-46
MsG0180000470.01	MsG0880044257.01	0.829019	6.506519e-55	6.577120e-52
MsG0180000702.01	MsG0880044257.01	0.816369	5.729254e-52	4.052119e-49
MsG0180002952.01	MsG0880044257.01	0.805559	1.258881e-49	6.680761e-47
MsG0180004275.01	MsG0880044257.01	0.822543	2.238991e-53	1.879785e-50
MsG0180004295.01	MsG0880044257.01	0.808667	2.767095e-50	1.591611e-47
MsG0180004331.01	MsG0880044257.01	0.821313	4.315603e-53	3.498728e-50
MsG0180004465.01	MsG0880044257.01	0.815502	8.948371e-52	6.180538e-49

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044257.01.T01	MTR_2g078670	54.795	73	32	1	69	140	1	73	6.36e-15	71.2
MsG0880044257.01.T01	MTR_7g059070	96.970	33	1	0	332	364	54	86	3.88e-14	69.3
MsG0880044257.01.T01	MTR_5g029820	96.970	33	1	0	332	364	54	86	3.88e-14	69.3
MsG0880044257.01.T01	MTR_5g029770	96.970	33	1	0	332	364	54	86	3.88e-14	69.3
MsG0880044257.01.T01	MTR_4g065990	96.970	33	1	0	332	364	54	86	3.88e-14	69.3
MsG0880044257.01.T01	MTR_4g088150	96.970	33	1	0	332	364	54	86	3.88e-14	69.3
MsG0880044257.01.T01	MTR_2g035230	96.970	33	1	0	332	364	54	86	3.88e-14	69.3
MsG0880044257.01.T01	MTR_8g092720	96.970	33	1	0	332	364	54	86	3.88e-14	69.3
MsG0880044257.01.T01	MTR_8g063500	96.970	33	1	0	332	364	54	86	3.88e-14	69.3
MsG0880044257.01.T01	MTR_8g103245	96.970	33	1	0	332	364	54	86	3.88e-14	69.3

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044257.01.T01	AT5G65360	96.970	33	1	332	364	54	86	3.76e-14	69.3
MsG0880044257.01.T01	AT5G10390	96.970	33	1	332	364	54	86	3.76e-14	69.3
MsG0880044257.01.T01	AT5G10400	96.970	33	1	332	364	54	86	3.76e-14	69.3
MsG0880044257.01.T01	AT3G27360	96.970	33	1	332	364	54	86	3.76e-14	69.3
MsG0880044257.01.T01	AT1G09200	96.970	33	1	332	364	54	86	3.76e-14	69.3
MsG0880044257.01.T01	AT5G10980	96.970	33	1	332	364	54	86	4.80e-14	69.3
MsG0880044257.01.T01	AT5G10980	90.000	30	3	38	67	22	51	1.44e-11	62.0
MsG0880044257.01.T01	AT4G40040	96.970	33	1	332	364	54	86	4.80e-14	69.3
MsG0880044257.01.T01	AT4G40040	90.000	30	3	38	67	22	51	1.44e-11	62.0
MsG0880044257.01.T01	AT4G40040	96.970	33	1	332	364	54	86	4.80e-14	69.3

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Find 83 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATACCAAATTGGAGGGA+AGG	0.560350	8:+38716617	MsG0880044257.01.T01:CDS
AAACACAAAAGTTCCAACAG+TGG	0.740399	8:-38716395	None:intergenic
AAACCTCACCGTTACCGTCC+TGG	0.463835	8:+38715208	MsG0880044257.01.T01:CDS
AAATATGAAGTAAGTGAGGA+GGG	0.552565	8:+38715947	MsG0880044257.01.T01:CDS
AAATCTGCTCCTACTACTGG+AGG	0.621038	8:+38715178	MsG0880044257.01.T01:CDS
AACGCGGAATAGGAATTGTG+GGG	0.703065	8:-38714680	None:intergenic
AAGAATCTTTGTGGTGGAGC+TGG	0.437208	8:+38715401	MsG0880044257.01.T01:CDS
AAGAATGCTTTGCAGCAACG+CGG	0.716589	8:-38714696	None:intergenic
AATAACACACACTTGTAATA+TGG	0.222581	8:-38716466	None:intergenic
AATCATGTGATTGGGAATGT+TGG	0.511180	8:+38716059	MsG0880044257.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATATGAAGTAAGTGAGGA+GGG	+	Chr8:38715947-38715966	MsG0880044257.01.T01:CDS	30.0%
AACATAGAAGTCTTTACGAA+CGG	-	Chr8:38715897-38715916	None:intergenic	30.0%
AACATAGAAGTGATAGCATT+TGG	-	Chr8:38715868-38715887	None:intergenic	30.0%
ACTTACTTCATATTTGTACC+CGG	-	Chr8:38715942-38715961	None:intergenic	30.0%
AGAGAAAATACCAAATTGGA+GGG	+	Chr8:38716613-38716632	MsG0880044257.01.T01:CDS	30.0%
ATGAAGCCAAAAACCATAAT+AGG	-	Chr8:38716185-38716204	None:intergenic	30.0%
CTCTGTGTATAACTTTGAAT+TGG	+	Chr8:38716670-38716689	MsG0880044257.01.T01:CDS	30.0%
CTGTAAATAACATCAATCGA+AGG	-	Chr8:38715369-38715388	None:intergenic	30.0%
GAAAAACAAATCAGTTACAG+CGG	-	Chr8:38715077-38715096	None:intergenic	30.0%
GTACAAATATGAAGTAAGTG+AGG	+	Chr8:38715943-38715962	MsG0880044257.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	38714655	38717103	38714655	ID=MsG0880044257.01;Name=MsG0880044257.01
Chr8	mRNA	38714655	38717103	38714655	ID=MsG0880044257.01.T01;Parent=MsG0880044257.01;Name=MsG0880044257.01.T01;_AED=0.64;_eAED=0.71;_QI=0\|0\|0\|0.42\|1\|1\|7\|0\|387
Chr8	exon	38714655	38714774	38714655	ID=MsG0880044257.01.T01:exon:2482;Parent=MsG0880044257.01.T01
Chr8	exon	38715169	38715245	38715169	ID=MsG0880044257.01.T01:exon:2481;Parent=MsG0880044257.01.T01
Chr8	exon	38715389	38715491	38715389	ID=MsG0880044257.01.T01:exon:2480;Parent=MsG0880044257.01.T01
Chr8	exon	38715926	38716476	38715926	ID=MsG0880044257.01.T01:exon:2479;Parent=MsG0880044257.01.T01
Chr8	exon	38716559	38716709	38716559	ID=MsG0880044257.01.T01:exon:2478;Parent=MsG0880044257.01.T01
Chr8	exon	38716841	38716912	38716841	ID=MsG0880044257.01.T01:exon:2477;Parent=MsG0880044257.01.T01
Chr8	exon	38717014	38717103	38717014	ID=MsG0880044257.01.T01:exon:2476;Parent=MsG0880044257.01.T01
Chr8	CDS	38714655	38714774	38714655	ID=MsG0880044257.01.T01:cds;Parent=MsG0880044257.01.T01
Chr8	CDS	38715169	38715245	38715169	ID=MsG0880044257.01.T01:cds;Parent=MsG0880044257.01.T01
Chr8	CDS	38715389	38715491	38715389	ID=MsG0880044257.01.T01:cds;Parent=MsG0880044257.01.T01
Chr8	CDS	38715926	38716476	38715926	ID=MsG0880044257.01.T01:cds;Parent=MsG0880044257.01.T01
Chr8	CDS	38716559	38716709	38716559	ID=MsG0880044257.01.T01:cds;Parent=MsG0880044257.01.T01
Chr8	CDS	38716841	38716912	38716841	ID=MsG0880044257.01.T01:cds;Parent=MsG0880044257.01.T01
Chr8	CDS	38717014	38717103	38717014	ID=MsG0880044257.01.T01:cds;Parent=MsG0880044257.01.T01

Gene Sequence

>MsG0880044257.01.T01

ATGATCTTGTGGTTCTCAACTCATACCCCACAATTCCTATTCCGCGTTGCTGCAAAGCATTCTTCACCCTTCTCTCTCTGTCTTTGCGATTTCTGCTACATCAACTTTTCTTCTTCCAAGGCTGCTAGGAAATCTGCTCCTACTACTGGAGGAGCCAAAAAACCTCACCGTTACCGTCCTGGAACCGTCGCACTTCGTGAGAAAATGGGAAGAATCTTTGTGGTGGAGCTGGAGGGCAGGTCCTATAGATGCAAATTCTGCAGAACTCATTTGGCTCTTGCAGATGATCTCATTTCTCGGATGAATAAGAAAGCCGGGTACAAATATGAAGTAAGTGAGGAGGGAAATCACATTTTTGGCAAAGTGACTCATTTTGGGTTGATTTCAACTTTCCCTTTATATAAATTGATGTTTAATTGCTTCTCTAGAGCTGAATCATGTGATTGGGAATGTTGGAATACCTTCTACAATCTTGAGGACAAGCTTGGTCTTCATGCGGTAGATAGTGATAAAATGTGCTCTCTGATTAATTTGTATGGAAGGGTCTTGTATGTTTCCTATTATGGTTTTTGGCTTCATTTCAGTATAGTCACTATAAATTGTTGCACGTTAGCTCAACCTTTCCATCTTGCAAGTTCTATGATCCAAACTCTACTGCAGAAGGATATATTGTTTGGTTCAGAAGCTTCAAATGAGCACTCTCAAAGGAAGCATGTCATGGGTCTACAATTAGCTACAAATGAACTCTGTCTTGAGCTCATTTTTCGTGCCACTGTTGGAACTTTTGTGTTTTCATATGATCCATTTGCTATTGTCACTGATAGTTCTTGTGGTGTTTACCCATATTACAATTTTGAATTTGGTATTTGGCTTAAGAGTGATAGCTTTAAAGACAATAACGTGCTAGAGAAAATACCAAATTGGAGGGAAGGAACTTATGAGTATGGCCAAACATTCATTGACTCTGTGTATAACTTTGAATTGGTTAAGGCTAAATATTCTAAGAGTACTGAGCTTTTGATCCGCAAGCTTCCATTTCAGCGTCTTGTCCGTGAAATTGCTCAAGATTTCAAGACGGATCTGAGATTCCAGAGGACATTCAGCTTGCTCGTCGGATTCGCGGTGAACGTGCTTAGGGTGGTGAAGGTGCTTTTAGGGTTATGA

Protein sequence

>MsG0880044257.01.T01

MILWFSTHTPQFLFRVAAKHSSPFSLCLCDFCYINFSSSKAARKSAPTTGGAKKPHRYRPGTVALREKMGRIFVVELEGRSYRCKFCRTHLALADDLISRMNKKAGYKYEVSEEGNHIFGKVTHFGLISTFPLYKLMFNCFSRAESCDWECWNTFYNLEDKLGLHAVDSDKMCSLINLYGRVLYVSYYGFWLHFSIVTINCCTLAQPFHLASSMIQTLLQKDILFGSEASNEHSQRKHVMGLQLATNELCLELIFRATVGTFVFSYDPFAIVTDSSCGVYPYYNFEFGIWLKSDSFKDNNVLEKIPNWREGTYEYGQTFIDSVYNFELVKAKYSKSTELLIRKLPFQRLVREIAQDFKTDLRFQRTFSLLVGFAVNVLRVVKVLLGL*