Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046176.01.T01 | XP_003608809.3 | 98.477 | 197 | 3 | 0 | 1 | 197 | 29 | 225 | 9.36E-136 | 389 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046176.01.T01 | G7JLB7 | 98.477 | 197 | 3 | 0 | 1 | 197 | 29 | 225 | 4.47e-136 | 389 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047999.01 | MsG0880046176.01 | 0.846224 | 2.483936e-59 | 4.245491e-56 |
| MsG0080048036.01 | MsG0880046176.01 | 0.804167 | 2.459543e-49 | 1.259353e-46 |
| MsG0080048919.01 | MsG0880046176.01 | -0.807222 | 5.616103e-50 | 3.110620e-47 |
| MsG0080048986.01 | MsG0880046176.01 | -0.825563 | 4.377682e-54 | 4.004393e-51 |
| MsG0080049023.01 | MsG0880046176.01 | -0.802460 | 5.551748e-49 | 2.721070e-46 |
| MsG0080049066.01 | MsG0880046176.01 | -0.808564 | 2.911623e-50 | 1.670239e-47 |
| MsG0080049133.01 | MsG0880046176.01 | -0.823826 | 1.123843e-53 | 9.783908e-51 |
| MsG0180000322.01 | MsG0880046176.01 | -0.812632 | 3.844517e-51 | 2.457013e-48 |
| MsG0180000337.01 | MsG0880046176.01 | 0.857893 | 1.196980e-62 | 2.999663e-59 |
| MsG0180001116.01 | MsG0880046176.01 | -0.803178 | 3.946875e-49 | 1.970184e-46 |
| MsG0180002099.01 | MsG0880046176.01 | -0.840855 | 6.763325e-58 | 9.759849e-55 |
| MsG0880044570.01 | MsG0880046176.01 | 0.815693 | 8.113432e-52 | 5.633106e-49 |
| MsG0880045272.01 | MsG0880046176.01 | 0.875318 | 3.387451e-68 | 1.574502e-64 |
| MsG0880045369.01 | MsG0880046176.01 | -0.811420 | 7.064517e-51 | 4.370928e-48 |
| MsG0880045385.01 | MsG0880046176.01 | -0.815768 | 7.804379e-52 | 5.430089e-49 |
| MsG0880046010.01 | MsG0880046176.01 | -0.810425 | 1.160000e-50 | 6.989629e-48 |
| MsG0880046105.01 | MsG0880046176.01 | -0.806874 | 6.654043e-50 | 3.652325e-47 |
| MsG0880046176.01 | MsG0880046308.01 | 0.835635 | 1.495906e-56 | 1.840001e-53 |
| MsG0880046176.01 | MsG0880046341.01 | -0.817203 | 3.724698e-52 | 2.695101e-49 |
| MsG0880046176.01 | MsG0880046429.01 | 0.810847 | 9.404488e-51 | 5.731257e-48 |
| MsG0880046176.01 | MsG0880046469.01 | -0.806773 | 6.990096e-50 | 3.826692e-47 |
| MsG0880046176.01 | MsG0880046630.01 | -0.801178 | 1.017768e-48 | 4.828775e-46 |
| MsG0880046176.01 | MsG0880046996.01 | 0.814007 | 1.918499e-51 | 1.272322e-48 |
| MsG0880046176.01 | MsG0880047486.01 | 0.821296 | 4.355053e-53 | 3.529002e-50 |
| MsG0880046176.01 | MsG0880047493.01 | 0.829344 | 5.425723e-55 | 5.538547e-52 |
| MsG0880046176.01 | MsG0880047627.01 | -0.803636 | 3.171978e-49 | 1.602112e-46 |
| MsG0180002981.01 | MsG0880046176.01 | 0.801119 | 1.046486e-48 | 4.957740e-46 |
| MsG0180003351.01 | MsG0880046176.01 | -0.807921 | 3.991575e-50 | 2.251328e-47 |
| MsG0180003757.01 | MsG0880046176.01 | -0.822092 | 2.849782e-53 | 2.361724e-50 |
| MsG0180003758.01 | MsG0880046176.01 | -0.816149 | 6.416810e-52 | 4.511165e-49 |
| MsG0180003830.01 | MsG0880046176.01 | 0.814290 | 1.660741e-51 | 1.109922e-48 |
| MsG0180004266.01 | MsG0880046176.01 | 0.806271 | 8.922263e-50 | 4.822381e-47 |
| MsG0180004502.01 | MsG0880046176.01 | -0.834746 | 2.507892e-56 | 3.003368e-53 |
| MsG0180004773.01 | MsG0880046176.01 | -0.835160 | 1.972625e-56 | 2.392173e-53 |
| MsG0180005272.01 | MsG0880046176.01 | 0.860628 | 1.808611e-63 | 4.975996e-60 |
| MsG0180005328.01 | MsG0880046176.01 | 0.859528 | 3.886920e-63 | 1.029715e-59 |
| MsG0180005474.01 | MsG0880046176.01 | -0.817803 | 2.727942e-52 | 2.006713e-49 |
| MsG0180005542.01 | MsG0880046176.01 | 0.824100 | 9.691662e-54 | 8.501889e-51 |
| MsG0180006227.01 | MsG0880046176.01 | -0.818616 | 1.786508e-52 | 1.343765e-49 |
| MsG0280006381.01 | MsG0880046176.01 | -0.812268 | 4.618289e-51 | 2.923072e-48 |
| MsG0780040806.01 | MsG0880046176.01 | 0.802906 | 4.491504e-49 | 2.226603e-46 |
| MsG0780041254.01 | MsG0880046176.01 | -0.804411 | 2.188245e-49 | 1.127446e-46 |
| MsG0780041316.01 | MsG0880046176.01 | 0.828962 | 6.715780e-55 | 6.777355e-52 |
| MsG0780041648.01 | MsG0880046176.01 | 0.815929 | 7.184345e-52 | 5.020244e-49 |
| MsG0780041663.01 | MsG0880046176.01 | 0.804342 | 2.262142e-49 | 1.163484e-46 |
| MsG0780041691.01 | MsG0880046176.01 | -0.806529 | 7.871410e-50 | 4.282283e-47 |
| MsG0780041783.01 | MsG0880046176.01 | -0.823576 | 1.286197e-53 | 1.111775e-50 |
| MsG0880041855.01 | MsG0880046176.01 | -0.807017 | 6.206405e-50 | 3.419207e-47 |
| MsG0880042495.01 | MsG0880046176.01 | 0.832962 | 7.004688e-56 | 7.952896e-53 |
| MsG0880042740.01 | MsG0880046176.01 | 0.807237 | 5.576060e-50 | 3.089614e-47 |
| MsG0880042858.01 | MsG0880046176.01 | -0.804156 | 2.473169e-49 | 1.265975e-46 |
| MsG0880043744.01 | MsG0880046176.01 | 0.826995 | 1.997694e-54 | 1.903747e-51 |
| MsG0880043828.01 | MsG0880046176.01 | 0.870775 | 1.130824e-66 | 4.452633e-63 |
| MsG0880043916.01 | MsG0880046176.01 | 0.806647 | 7.431009e-50 | 4.054701e-47 |
| MsG0280010629.01 | MsG0880046176.01 | 0.807322 | 5.349356e-50 | 2.970601e-47 |
| MsG0280011165.01 | MsG0880046176.01 | 0.876525 | 1.303090e-68 | 6.337874e-65 |
| MsG0280011193.01 | MsG0880046176.01 | 0.858198 | 9.714100e-63 | 2.459356e-59 |
| MsG0280011203.01 | MsG0880046176.01 | 0.864666 | 1.031992e-64 | 3.266489e-61 |
| MsG0280011264.01 | MsG0880046176.01 | 0.832412 | 9.592156e-56 | 1.071359e-52 |
| MsG0280011267.01 | MsG0880046176.01 | 0.825815 | 3.815450e-54 | 3.515437e-51 |
| MsG0380013114.01 | MsG0880046176.01 | -0.813817 | 2.112548e-51 | 1.393829e-48 |
| MsG0380013272.01 | MsG0880046176.01 | 0.821580 | 3.744836e-53 | 3.058631e-50 |
| MsG0380013300.01 | MsG0880046176.01 | 0.854050 | 1.593605e-61 | 3.514642e-58 |
| MsG0380014639.01 | MsG0880046176.01 | -0.814708 | 1.342280e-51 | 9.072508e-49 |
| MsG0380014642.01 | MsG0880046176.01 | -0.822435 | 2.372610e-53 | 1.985809e-50 |
| MsG0380014735.01 | MsG0880046176.01 | -0.808318 | 3.285484e-50 | 1.872705e-47 |
| MsG0380014802.01 | MsG0880046176.01 | 0.836317 | 1.004577e-56 | 1.261595e-53 |
| MsG0680030960.01 | MsG0880046176.01 | 0.820232 | 7.646329e-53 | 6.015589e-50 |
| MsG0680030961.01 | MsG0880046176.01 | 0.812572 | 3.963095e-51 | 2.528717e-48 |
| MsG0680031240.01 | MsG0880046176.01 | 0.803351 | 3.633782e-49 | 1.821892e-46 |
| MsG0680035584.01 | MsG0880046176.01 | -0.811153 | 8.072813e-51 | 4.959623e-48 |
| MsG0480018494.01 | MsG0880046176.01 | -0.804234 | 2.381893e-49 | 1.221633e-46 |
| MsG0480019217.01 | MsG0880046176.01 | 0.801603 | 8.328082e-49 | 3.993598e-46 |
| MsG0480019228.01 | MsG0880046176.01 | 0.816661 | 4.928564e-52 | 3.513800e-49 |
| MsG0480019304.01 | MsG0880046176.01 | 0.824764 | 6.762071e-54 | 6.046790e-51 |
| MsG0480020411.01 | MsG0880046176.01 | 0.805734 | 1.157033e-49 | 6.168563e-47 |
| MsG0480020497.01 | MsG0880046176.01 | 0.808617 | 2.836685e-50 | 1.629421e-47 |
| MsG0480020838.01 | MsG0880046176.01 | -0.817655 | 2.946195e-52 | 2.158376e-49 |
| MsG0480021131.01 | MsG0880046176.01 | 0.830995 | 2.145502e-55 | 2.297834e-52 |
| MsG0480021169.01 | MsG0880046176.01 | -0.805128 | 1.550344e-49 | 8.137171e-47 |
| MsG0480021327.01 | MsG0880046176.01 | -0.813280 | 2.772234e-51 | 1.802597e-48 |
| MsG0480022125.01 | MsG0880046176.01 | 0.829728 | 4.374907e-55 | 4.516232e-52 |
| MsG0480022383.01 | MsG0880046176.01 | 0.818458 | 1.939742e-52 | 1.452902e-49 |
| MsG0480022384.01 | MsG0880046176.01 | 0.805318 | 1.414750e-49 | 7.461638e-47 |
| MsG0480022400.01 | MsG0880046176.01 | 0.812218 | 4.736181e-51 | 2.993668e-48 |
| MsG0480022655.01 | MsG0880046176.01 | 0.864608 | 1.075849e-64 | 3.398344e-61 |
| MsG0480022935.01 | MsG0880046176.01 | -0.817097 | 3.934647e-52 | 2.838724e-49 |
| MsG0480023107.01 | MsG0880046176.01 | 0.842911 | 1.935874e-58 | 2.978732e-55 |
| MsG0480023152.01 | MsG0880046176.01 | -0.815540 | 8.771731e-52 | 6.064776e-49 |
| MsG0480023267.01 | MsG0880046176.01 | 0.828626 | 8.096168e-55 | 8.091615e-52 |
| MsG0480023303.01 | MsG0880046176.01 | 0.829630 | 4.623238e-55 | 4.758393e-52 |
| MsG0480023695.01 | MsG0880046176.01 | -0.831768 | 1.384055e-55 | 1.516967e-52 |
| MsG0480023818.01 | MsG0880046176.01 | -0.801874 | 7.327837e-49 | 3.538534e-46 |
| MsG0480023921.01 | MsG0880046176.01 | 0.802231 | 6.188765e-49 | 3.015589e-46 |
| MsG0580024398.01 | MsG0880046176.01 | 0.846850 | 1.676390e-59 | 2.924002e-56 |
| MsG0580024408.01 | MsG0880046176.01 | 0.812855 | 3.436164e-51 | 2.208777e-48 |
| MsG0580024442.01 | MsG0880046176.01 | -0.810930 | 9.020432e-51 | 5.509861e-48 |
| MsG0580024493.01 | MsG0880046176.01 | -0.808957 | 2.398802e-50 | 1.390394e-47 |
| MsG0580025105.01 | MsG0880046176.01 | -0.816936 | 4.275722e-52 | 3.071496e-49 |
| MsG0580025151.01 | MsG0880046176.01 | 0.802189 | 6.312433e-49 | 3.072492e-46 |
| MsG0380015010.01 | MsG0880046176.01 | 0.824051 | 9.952829e-54 | 8.718654e-51 |
| MsG0380015369.01 | MsG0880046176.01 | 0.802058 | 6.717619e-49 | 3.258941e-46 |
| MsG0380015572.01 | MsG0880046176.01 | 0.835564 | 1.559054e-56 | 1.913577e-53 |
| MsG0380015654.01 | MsG0880046176.01 | -0.823055 | 1.701712e-53 | 1.449566e-50 |
| MsG0380016476.01 | MsG0880046176.01 | 0.801863 | 7.365164e-49 | 3.555541e-46 |
| MsG0380016839.01 | MsG0880046176.01 | 0.820503 | 6.629017e-53 | 5.254479e-50 |
| MsG0380016933.01 | MsG0880046176.01 | -0.801620 | 8.263845e-49 | 3.964508e-46 |
| MsG0380016934.01 | MsG0880046176.01 | -0.803235 | 3.841202e-49 | 1.920187e-46 |
| MsG0380016935.01 | MsG0880046176.01 | -0.813402 | 2.607000e-51 | 1.700877e-48 |
| MsG0380017247.01 | MsG0880046176.01 | 0.810339 | 1.210751e-50 | 7.278964e-48 |
| MsG0380017405.01 | MsG0880046176.01 | -0.822226 | 2.652785e-53 | 2.206964e-50 |
| MsG0380017406.01 | MsG0880046176.01 | -0.801016 | 1.098428e-48 | 5.190558e-46 |
| MsG0380017812.01 | MsG0880046176.01 | -0.835956 | 1.240714e-56 | 1.540909e-53 |
| MsG0380017928.01 | MsG0880046176.01 | -0.817382 | 3.395121e-52 | 2.468827e-49 |
| MsG0380018006.01 | MsG0880046176.01 | 0.834660 | 2.635482e-56 | 3.148169e-53 |
| MsG0380018051.01 | MsG0880046176.01 | 0.832945 | 7.075991e-56 | 8.029466e-53 |
| MsG0480018201.01 | MsG0880046176.01 | -0.820582 | 6.355436e-53 | 5.048515e-50 |
| MsG0480018202.01 | MsG0880046176.01 | -0.813341 | 2.688363e-51 | 1.751003e-48 |
| MsG0480018203.01 | MsG0880046176.01 | -0.811464 | 6.909749e-51 | 4.280164e-48 |
| MsG0580027646.01 | MsG0880046176.01 | 0.827641 | 1.398168e-54 | 1.357686e-51 |
| MsG0580027797.01 | MsG0880046176.01 | 0.824362 | 8.411888e-54 | 7.434539e-51 |
| MsG0580028999.01 | MsG0880046176.01 | 0.824858 | 6.426190e-54 | 5.761763e-51 |
| MsG0580030133.01 | MsG0880046176.01 | 0.818367 | 2.034509e-52 | 1.520012e-49 |
| MsG0580030135.01 | MsG0880046176.01 | 0.802242 | 6.155434e-49 | 3.000262e-46 |
| MsG0580030197.01 | MsG0880046176.01 | -0.807917 | 3.999004e-50 | 2.255266e-47 |
| MsG0680030315.01 | MsG0880046176.01 | 0.847734 | 9.585741e-60 | 1.720086e-56 |
| MsG0680030545.01 | MsG0880046176.01 | 0.811766 | 5.941255e-51 | 3.709581e-48 |
| MsG0280006700.01 | MsG0880046176.01 | 0.811283 | 7.564004e-51 | 4.662942e-48 |
| MsG0280006883.01 | MsG0880046176.01 | 0.849914 | 2.381461e-60 | 4.585821e-57 |
| MsG0280006973.01 | MsG0880046176.01 | -0.815404 | 9.408053e-52 | 6.480246e-49 |
| MsG0280007017.01 | MsG0880046176.01 | -0.800976 | 1.119561e-48 | 5.284853e-46 |
| MsG0280007201.01 | MsG0880046176.01 | 0.812264 | 4.626090e-51 | 2.927732e-48 |
| MsG0280007216.01 | MsG0880046176.01 | 0.813260 | 2.800761e-51 | 1.820075e-48 |
| MsG0280007288.01 | MsG0880046176.01 | -0.802854 | 4.605009e-49 | 2.279810e-46 |
| MsG0280007291.01 | MsG0880046176.01 | -0.807373 | 5.218166e-50 | 2.901465e-47 |
| MsG0280007759.01 | MsG0880046176.01 | 0.802757 | 4.821006e-49 | 2.380895e-46 |
| MsG0280008405.01 | MsG0880046176.01 | 0.858152 | 1.002269e-62 | 2.533531e-59 |
| MsG0280009079.01 | MsG0880046176.01 | -0.805025 | 1.628757e-49 | 8.525964e-47 |
| MsG0280009094.01 | MsG0880046176.01 | -0.808601 | 2.858358e-50 | 1.641276e-47 |
| MsG0280009394.01 | MsG0880046176.01 | 0.851098 | 1.107472e-60 | 2.216814e-57 |
| MsG0280009742.01 | MsG0880046176.01 | -0.809596 | 1.749718e-50 | 1.031687e-47 |
| MsG0280010019.01 | MsG0880046176.01 | 0.820980 | 5.149491e-53 | 4.136784e-50 |
| MsG0280010047.01 | MsG0880046176.01 | 0.851635 | 7.808007e-61 | 1.590341e-57 |
| MsG0280010053.01 | MsG0880046176.01 | 0.838988 | 2.073251e-57 | 2.823871e-54 |
| MsG0280010055.01 | MsG0880046176.01 | 0.810410 | 1.168700e-50 | 7.039388e-48 |
| MsG0280010227.01 | MsG0880046176.01 | -0.829292 | 5.586697e-55 | 5.694472e-52 |
| MsG0280010240.01 | MsG0880046176.01 | 0.849719 | 2.699286e-60 | 5.164061e-57 |
| MsG0780035994.01 | MsG0880046176.01 | -0.824912 | 6.242516e-54 | 5.605685e-51 |
| MsG0780036661.01 | MsG0880046176.01 | 0.837516 | 4.963421e-57 | 6.463431e-54 |
| MsG0780037182.01 | MsG0880046176.01 | 0.809473 | 1.859767e-50 | 1.092920e-47 |
| MsG0780037698.01 | MsG0880046176.01 | -0.815888 | 7.338038e-52 | 5.121771e-49 |
| MsG0780038047.01 | MsG0880046176.01 | -0.800011 | 1.760702e-48 | 8.109726e-46 |
| MsG0780039190.01 | MsG0880046176.01 | 0.800409 | 1.460950e-48 | 6.797754e-46 |
| MsG0780039783.01 | MsG0880046176.01 | 0.803152 | 3.996137e-49 | 1.993455e-46 |
| MsG0780039838.01 | MsG0880046176.01 | -0.826154 | 3.169115e-54 | 2.948117e-51 |
| MsG0780039839.01 | MsG0880046176.01 | -0.844498 | 7.283835e-59 | 1.178175e-55 |
| MsG0780039840.01 | MsG0880046176.01 | 0.803674 | 3.114959e-49 | 1.574793e-46 |
| MsG0780039969.01 | MsG0880046176.01 | 0.861133 | 1.270318e-63 | 3.556869e-60 |
| MsG0780039978.01 | MsG0880046176.01 | 0.858099 | 1.039571e-62 | 2.623209e-59 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046176.01.T01 | MTR_4g102220 | 98.477 | 197 | 3 | 0 | 1 | 197 | 29 | 225 | 1.13e-139 | 389 |
| MsG0880046176.01.T01 | MTR_5g011800 | 61.386 | 202 | 66 | 5 | 2 | 197 | 34 | 229 | 2.04e-75 | 226 |
| MsG0880046176.01.T01 | MTR_5g011820 | 57.843 | 204 | 73 | 6 | 1 | 197 | 35 | 232 | 2.59e-69 | 211 |
| MsG0880046176.01.T01 | MTR_4g024370 | 52.239 | 201 | 84 | 7 | 1 | 196 | 38 | 231 | 8.97e-60 | 187 |
| MsG0880046176.01.T01 | MTR_4g024370 | 52.239 | 201 | 84 | 7 | 1 | 196 | 38 | 231 | 8.97e-60 | 187 |
| MsG0880046176.01.T01 | MTR_1g076800 | 31.343 | 201 | 116 | 7 | 1 | 196 | 28 | 211 | 2.26e-17 | 77.4 |
| MsG0880046176.01.T01 | MTR_6g060390 | 28.218 | 202 | 122 | 8 | 1 | 196 | 28 | 212 | 7.72e-17 | 75.9 |
| MsG0880046176.01.T01 | MTR_6g060440 | 27.363 | 201 | 123 | 7 | 1 | 196 | 28 | 210 | 9.38e-17 | 75.5 |
| MsG0880046176.01.T01 | MTR_4g023950 | 31.000 | 200 | 114 | 8 | 1 | 196 | 28 | 207 | 8.53e-16 | 73.2 |
| MsG0880046176.01.T01 | MTR_7g117380 | 31.000 | 200 | 120 | 9 | 3 | 196 | 34 | 221 | 1.04e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046176.01.T01 | AT2G45360 | 51.515 | 198 | 84 | 4 | 1 | 197 | 29 | 215 | 3.25e-55 | 174 |
| MsG0880046176.01.T01 | AT3G60780 | 49.000 | 200 | 89 | 5 | 1 | 197 | 29 | 218 | 3.50e-54 | 172 |
| MsG0880046176.01.T01 | AT1G62840 | 44.845 | 194 | 92 | 5 | 11 | 197 | 39 | 224 | 1.78e-45 | 150 |
| MsG0880046176.01.T01 | AT1G12320 | 42.021 | 188 | 90 | 5 | 11 | 195 | 39 | 210 | 1.07e-36 | 127 |
Find 41 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTAGATTGTGAAGGAATTA+AGG | 0.263734 | 8:-70187539 | MsG0880046176.01.T01:CDS |
| CATTGAAGAAATAGATTTCA+TGG | 0.318994 | 8:-70187563 | MsG0880046176.01.T01:CDS |
| TAAGGTCACGTCGTGTTGTT+AGG | 0.340628 | 8:-70187393 | MsG0880046176.01.T01:CDS |
| TAGCACATGTTTCAGCTGTT+GGG | 0.366973 | 8:-70187336 | MsG0880046176.01.T01:CDS |
| GGTGGCTAAGTTAAGTGTTA+AGG | 0.386848 | 8:-70187497 | MsG0880046176.01.T01:CDS |
| TTGTTGAGGAAGGAGGATTA+AGG | 0.422749 | 8:-70187411 | MsG0880046176.01.T01:CDS |
| AGGTCGGTGTTTCTTCCTGT+TGG | 0.430254 | 8:-70187373 | MsG0880046176.01.T01:CDS |
| ATAGCAAGAAAACACACAAA+TGG | 0.451783 | 8:-70187706 | MsG0880046176.01.T01:CDS |
| AAGCATGTTGATCAACGGTC+AGG | 0.453278 | 8:-70187262 | MsG0880046176.01.T01:CDS |
| AGGAATTAAGGATTTGATGA+AGG | 0.457401 | 8:-70187527 | MsG0880046176.01.T01:CDS |
| ATGTCAAGTCCCTTCCCAAC+AGG | 0.460059 | 8:+70187358 | None:intergenic |
| GGTCGGTGTTTCTTCCTGTT+GGG | 0.463683 | 8:-70187372 | MsG0880046176.01.T01:CDS |
| GTGGCTAAGTTAAGTGTTAA+GGG | 0.463703 | 8:-70187496 | MsG0880046176.01.T01:CDS |
| GTGTTTCTTCCTGTTGGGAA+GGG | 0.468303 | 8:-70187367 | MsG0880046176.01.T01:CDS |
| ATAGCACATGTTTCAGCTGT+TGG | 0.469433 | 8:-70187337 | MsG0880046176.01.T01:CDS |
| GGTGTTTCTTCCTGTTGGGA+AGG | 0.479058 | 8:-70187368 | MsG0880046176.01.T01:CDS |
| TAGCAAGAAAACACACAAAT+GGG | 0.488210 | 8:-70187705 | MsG0880046176.01.T01:CDS |
| CTGATTTCAGCTATGGCAGC+AGG | 0.506373 | 8:-70187799 | MsG0880046176.01.T01:CDS |
| ACATCAACAGAAATCATTGT+AGG | 0.506787 | 8:-70187613 | MsG0880046176.01.T01:CDS |
| GGTGTAATCGAAACAAGCTT+AGG | 0.508623 | 8:-70187736 | MsG0880046176.01.T01:CDS |
| AGCACATGTTTCAGCTGTTG+GGG | 0.510606 | 8:-70187335 | MsG0880046176.01.T01:CDS |
| GATATTGTTGTTGAGGAAGG+AGG | 0.516444 | 8:-70187418 | MsG0880046176.01.T01:CDS |
| GGCGGAGCTGATTTCAGCTA+TGG | 0.519908 | 8:-70187806 | MsG0880046176.01.T01:CDS |
| ATGGGAGGATATTGTTGTTG+AGG | 0.526267 | 8:-70187425 | MsG0880046176.01.T01:CDS |
| GGAAAAGATGGTCATGGTCG+TGG | 0.531446 | 8:-70187304 | MsG0880046176.01.T01:CDS |
| GTCACGTCGTGTTGTTAGGT+CGG | 0.536069 | 8:-70187389 | MsG0880046176.01.T01:CDS |
| AGCATGTTGATCAACGGTCA+GGG | 0.549561 | 8:-70187261 | MsG0880046176.01.T01:CDS |
| GAGGATATTGTTGTTGAGGA+AGG | 0.561976 | 8:-70187421 | MsG0880046176.01.T01:CDS |
| GTCGTGGTGGTAGTAAGAGA+TGG | 0.579545 | 8:-70187288 | MsG0880046176.01.T01:CDS |
| ATAGTAGAAACATGGTCAGA+AGG | 0.588053 | 8:-70187760 | MsG0880046176.01.T01:CDS |
| TTTCAGCTATGGCAGCAGGA+TGG | 0.632513 | 8:-70187795 | MsG0880046176.01.T01:CDS |
| GCATGTTGATCAACGGTCAG+GGG | 0.633202 | 8:-70187260 | MsG0880046176.01.T01:CDS |
| GGATTAAGCATGTTGATCAA+CGG | 0.643256 | 8:-70187267 | MsG0880046176.01.T01:CDS |
| TTCATGGTGGTAGATTGTGA+AGG | 0.645943 | 8:-70187547 | MsG0880046176.01.T01:CDS |
| TGTTGATCAACGGTCAGGGG+AGG | 0.647427 | 8:-70187257 | MsG0880046176.01.T01:CDS |
| GTAGAAACATGGTCAGAAGG+AGG | 0.669642 | 8:-70187757 | MsG0880046176.01.T01:CDS |
| AAAGATGGTCATGGTCGTGG+TGG | 0.675881 | 8:-70187301 | MsG0880046176.01.T01:CDS |
| AATTTGGGAAAAGATGGTCA+TGG | 0.694637 | 8:-70187310 | MsG0880046176.01.T01:CDS |
| CGTATGCATAGTTCCAAACG+AGG | 0.704080 | 8:-70187677 | MsG0880046176.01.T01:CDS |
| GCACATGTTTCAGCTGTTGG+GGG | 0.717069 | 8:-70187334 | MsG0880046176.01.T01:CDS |
| TGAAGAAATAGATTTCATGG+TGG | 0.736087 | 8:-70187560 | MsG0880046176.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATGTAAGAATGTTAATTTT+AGG | - | Chr8:70187583-70187602 | MsG0880046176.01.T01:CDS | 15.0% |
| !!! | ATTAGAGTACTCTAAAAAAA+TGG | - | Chr8:70187398-70187417 | MsG0880046176.01.T01:CDS | 20.0% |
| !!! | TTAGAGTACTCTAAAAAAAT+GGG | - | Chr8:70187399-70187418 | MsG0880046176.01.T01:CDS | 20.0% |
| ! | CAAAAATGATAGTAGAAACA+TGG | - | Chr8:70187277-70187296 | MsG0880046176.01.T01:CDS | 25.0% |
| ! | CATTGAAGAAATAGATTTCA+TGG | - | Chr8:70187482-70187501 | MsG0880046176.01.T01:CDS | 25.0% |
| !!! | AAGAATGTTAATTTTAGGAG+TGG | - | Chr8:70187588-70187607 | MsG0880046176.01.T01:CDS | 25.0% |
| !!! | AGAATGTTAATTTTAGGAGT+GGG | - | Chr8:70187589-70187608 | MsG0880046176.01.T01:CDS | 25.0% |
| ACATCAACAGAAATCATTGT+AGG | - | Chr8:70187432-70187451 | MsG0880046176.01.T01:CDS | 30.0% | |
| AGGAATTAAGGATTTGATGA+AGG | - | Chr8:70187518-70187537 | MsG0880046176.01.T01:CDS | 30.0% | |
| ATAGCAAGAAAACACACAAA+TGG | - | Chr8:70187339-70187358 | MsG0880046176.01.T01:CDS | 30.0% | |
| TAGCAAGAAAACACACAAAT+GGG | - | Chr8:70187340-70187359 | MsG0880046176.01.T01:CDS | 30.0% | |
| TCTAAAAAAATGGGTGAACA+AGG | - | Chr8:70187408-70187427 | MsG0880046176.01.T01:CDS | 30.0% | |
| TGAAGAAATAGATTTCATGG+TGG | - | Chr8:70187485-70187504 | MsG0880046176.01.T01:CDS | 30.0% | |
| ! | TAGGAGTGGGAATTTTAAAT+GGG | - | Chr8:70187602-70187621 | MsG0880046176.01.T01:CDS | 30.0% |
| ! | TTAGGAGTGGGAATTTTAAA+TGG | - | Chr8:70187601-70187620 | MsG0880046176.01.T01:CDS | 30.0% |
| AATTTGGGAAAAGATGGTCA+TGG | - | Chr8:70187735-70187754 | MsG0880046176.01.T01:CDS | 35.0% | |
| ATAGTAGAAACATGGTCAGA+AGG | - | Chr8:70187285-70187304 | MsG0880046176.01.T01:CDS | 35.0% | |
| GGATTAAGCATGTTGATCAA+CGG | - | Chr8:70187778-70187797 | MsG0880046176.01.T01:CDS | 35.0% | |
| GGTAGATTGTGAAGGAATTA+AGG | - | Chr8:70187506-70187525 | MsG0880046176.01.T01:CDS | 35.0% | |
| GTGGCTAAGTTAAGTGTTAA+GGG | - | Chr8:70187549-70187568 | MsG0880046176.01.T01:CDS | 35.0% | |
| !! | GGATTTGATGAAGGTTTTGA+AGG | - | Chr8:70187527-70187546 | MsG0880046176.01.T01:CDS | 35.0% |
| !! | TCTAATTTTGATGCCTCGTT+TGG | + | Chr8:70187384-70187403 | None:intergenic | 35.0% |
| !!! | TTTGATGAAGGTTTTGAAGG+TGG | - | Chr8:70187530-70187549 | MsG0880046176.01.T01:CDS | 35.0% |
| ATAGCACATGTTTCAGCTGT+TGG | - | Chr8:70187708-70187727 | MsG0880046176.01.T01:CDS | 40.0% | |
| GGTGGCTAAGTTAAGTGTTA+AGG | - | Chr8:70187548-70187567 | MsG0880046176.01.T01:CDS | 40.0% | |
| GGTGTAATCGAAACAAGCTT+AGG | - | Chr8:70187309-70187328 | MsG0880046176.01.T01:CDS | 40.0% | |
| TAGCACATGTTTCAGCTGTT+GGG | - | Chr8:70187709-70187728 | MsG0880046176.01.T01:CDS | 40.0% | |
| TTCATGGTGGTAGATTGTGA+AGG | - | Chr8:70187498-70187517 | MsG0880046176.01.T01:CDS | 40.0% | |
| TTGTTGAGGAAGGAGGATTA+AGG | - | Chr8:70187634-70187653 | MsG0880046176.01.T01:CDS | 40.0% | |
| ! | ATGGGAGGATATTGTTGTTG+AGG | - | Chr8:70187620-70187639 | MsG0880046176.01.T01:CDS | 40.0% |
| ! | GAGGATATTGTTGTTGAGGA+AGG | - | Chr8:70187624-70187643 | MsG0880046176.01.T01:CDS | 40.0% |
| ! | GAGTGGGAATTTTAAATGGG+AGG | - | Chr8:70187605-70187624 | MsG0880046176.01.T01:CDS | 40.0% |
| ! | GATATTGTTGTTGAGGAAGG+AGG | - | Chr8:70187627-70187646 | MsG0880046176.01.T01:CDS | 40.0% |
| AAGCATGTTGATCAACGGTC+AGG | - | Chr8:70187783-70187802 | MsG0880046176.01.T01:CDS | 45.0% | |
| AGCACATGTTTCAGCTGTTG+GGG | - | Chr8:70187710-70187729 | MsG0880046176.01.T01:CDS | 45.0% | |
| AGCATGTTGATCAACGGTCA+GGG | - | Chr8:70187784-70187803 | MsG0880046176.01.T01:CDS | 45.0% | |
| CGTATGCATAGTTCCAAACG+AGG | - | Chr8:70187368-70187387 | MsG0880046176.01.T01:CDS | 45.0% | |
| GGGGGTAATTTGGGAAAAGA+TGG | - | Chr8:70187729-70187748 | MsG0880046176.01.T01:CDS | 45.0% | |
| GTAGAAACATGGTCAGAAGG+AGG | - | Chr8:70187288-70187307 | MsG0880046176.01.T01:CDS | 45.0% | |
| GTGTTTCTTCCTGTTGGGAA+GGG | - | Chr8:70187678-70187697 | MsG0880046176.01.T01:CDS | 45.0% | |
| TCAGCTGTTGGGGGTAATTT+GGG | - | Chr8:70187720-70187739 | MsG0880046176.01.T01:CDS | 45.0% | |
| TTCAGCTGTTGGGGGTAATT+TGG | - | Chr8:70187719-70187738 | MsG0880046176.01.T01:CDS | 45.0% | |
| ! | TAAGGTCACGTCGTGTTGTT+AGG | - | Chr8:70187652-70187671 | MsG0880046176.01.T01:CDS | 45.0% |
| AAAGATGGTCATGGTCGTGG+TGG | - | Chr8:70187744-70187763 | MsG0880046176.01.T01:CDS | 50.0% | |
| ATGTCAAGTCCCTTCCCAAC+AGG | + | Chr8:70187690-70187709 | None:intergenic | 50.0% | |
| CTGATTTCAGCTATGGCAGC+AGG | - | Chr8:70187246-70187265 | MsG0880046176.01.T01:CDS | 50.0% | |
| GCACATGTTTCAGCTGTTGG+GGG | - | Chr8:70187711-70187730 | MsG0880046176.01.T01:CDS | 50.0% | |
| GCATGTTGATCAACGGTCAG+GGG | - | Chr8:70187785-70187804 | MsG0880046176.01.T01:CDS | 50.0% | |
| GGAAAAGATGGTCATGGTCG+TGG | - | Chr8:70187741-70187760 | MsG0880046176.01.T01:CDS | 50.0% | |
| GGTGTTTCTTCCTGTTGGGA+AGG | - | Chr8:70187677-70187696 | MsG0880046176.01.T01:CDS | 50.0% | |
| GTCGTGGTGGTAGTAAGAGA+TGG | - | Chr8:70187757-70187776 | MsG0880046176.01.T01:CDS | 50.0% | |
| TTTCAGCTATGGCAGCAGGA+TGG | - | Chr8:70187250-70187269 | MsG0880046176.01.T01:CDS | 50.0% | |
| ! | AGGTCGGTGTTTCTTCCTGT+TGG | - | Chr8:70187672-70187691 | MsG0880046176.01.T01:CDS | 50.0% |
| ! | GGTCGGTGTTTCTTCCTGTT+GGG | - | Chr8:70187673-70187692 | MsG0880046176.01.T01:CDS | 50.0% |
| ! | GTCACGTCGTGTTGTTAGGT+CGG | - | Chr8:70187656-70187675 | MsG0880046176.01.T01:CDS | 50.0% |
| TGTTGATCAACGGTCAGGGG+AGG | - | Chr8:70187788-70187807 | MsG0880046176.01.T01:CDS | 55.0% | |
| ! | GGCGGAGCTGATTTCAGCTA+TGG | - | Chr8:70187239-70187258 | MsG0880046176.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 70187237 | 70187830 | 70187237 | ID=MsG0880046176.01;Name=MsG0880046176.01 |
| Chr8 | mRNA | 70187237 | 70187830 | 70187237 | ID=MsG0880046176.01.T01;Parent=MsG0880046176.01;Name=MsG0880046176.01.T01;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|197 |
| Chr8 | exon | 70187237 | 70187830 | 70187237 | ID=MsG0880046176.01.T01:exon:27450;Parent=MsG0880046176.01.T01 |
| Chr8 | CDS | 70187237 | 70187830 | 70187237 | ID=MsG0880046176.01.T01:cds;Parent=MsG0880046176.01.T01 |
| Gene Sequence |
| Protein sequence |
