AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046599.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046599.01.T01 MTR_1g076780 80.892 157 8 2 1 135 1 157 1.27e-85 247
MsG0880046599.01.T01 MTR_4g111810 80.769 156 8 2 1 134 62 217 1.89e-81 245
MsG0880046599.01.T01 MTR_4g111810 80.769 156 8 2 1 134 62 217 2.14e-81 244
MsG0880046599.01.T01 MTR_4g077783 79.487 156 10 2 1 134 1 156 2.16e-79 243
MsG0880046599.01.T01 MTR_4g077783 75.000 124 9 2 33 134 11 134 7.12e-55 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046599.01.T01 AT2G34260 55.769 156 44 2 1 134 1 153 4.66e-49 160
MsG0880046599.01.T01 AT2G34260 70.690 58 17 0 77 134 39 96 6.39e-24 94.0

Find 40 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAGAAATGGAAATCAATT+TGG 0.133063 8:+75330193 MsG0880046599.01.T01:intron
GCAGAAATGGAAATCAATTT+GGG 0.263158 8:+75330194 MsG0880046599.01.T01:intron
AGACGATGATGGTTGTATTA+AGG 0.363138 8:+75331044 MsG0880046599.01.T01:CDS
ACTGAGTCAACCGTTGCTTC+AGG 0.370784 8:+75331021 MsG0880046599.01.T01:CDS
GTAATGCACTTTATAGCTCT+TGG 0.429900 8:+75330674 MsG0880046599.01.T01:CDS
TCTGAAAATCTCGTTGCTAC+AGG 0.460466 8:+75330248 MsG0880046599.01.T01:CDS
ATCTGATGCAATGAAACTAT+TGG 0.465061 8:+75331355 MsG0880046599.01.T01:CDS
GTTTGGAATTTATACCTCAC+TGG 0.467283 8:-75330444 None:intergenic
ACCGCGGCCGGAGGTTTCTC+TGG 0.469948 8:-75329596 None:intergenic
CAAGTTTATCAATCTATTGA+CGG 0.472270 8:-75330998 None:intergenic
GGAGAGAGCACGACCGCGGC+CGG 0.472362 8:-75329608 None:intergenic
GTTGCTACAGGACTCATAGA+TGG 0.479575 8:+75330260 MsG0880046599.01.T01:CDS
CGATTCATCAACGGCGGACG+TGG 0.494681 8:+75330652 MsG0880046599.01.T01:CDS
GAACAGTGAATGACGGATGC+CGG 0.505077 8:-75329629 None:intergenic
TTGTCTAAATTCCAGGGTGT+TGG 0.508482 8:+75330594 MsG0880046599.01.T01:intron
ATTGCAACAAGAACGTTCTC+TGG 0.509789 8:-75331288 None:intergenic
GGCCGCGGTCGTGCTCTCTC+CGG 0.511528 8:+75329610 MsG0880046599.01.T01:five_prime_UTR
ACCAGAGAAACCTCCGGCCG+CGG 0.515993 8:+75329595 MsG0880046599.01.T01:five_prime_UTR
AGCTCTGCAAGACTCGGTGT+GGG 0.536682 8:-75330626 None:intergenic
CAATTCATTTGAAGCTCACG+AGG 0.541421 8:+75331307 MsG0880046599.01.T01:CDS
TTTGGAATTTATACCTCACT+GGG 0.545001 8:-75330443 None:intergenic
GTTGCTTCAGGAGACGATGA+TGG 0.547346 8:+75331033 MsG0880046599.01.T01:CDS
GAAACTATTGGCCACAAGGT+AGG 0.557387 8:+75331367 MsG0880046599.01.T01:CDS
CAATGAAACTATTGGCCACA+AGG 0.574094 8:+75331363 MsG0880046599.01.T01:CDS
TCGAGCAGCTCTGCAAGACT+CGG 0.577288 8:-75330632 None:intergenic
CAGAAATGGAAATCAATTTG+GGG 0.582337 8:+75330195 MsG0880046599.01.T01:intron
CAGCTCTGCAAGACTCGGTG+TGG 0.588714 8:-75330627 None:intergenic
GGAAAACAACTTACAAGTGA+AGG 0.595999 8:-75330286 None:intergenic
TGCCGGAGAGAGCACGACCG+CGG 0.606529 8:-75329612 None:intergenic
ATCATCGTCTCCTGAAGCAA+CGG 0.608910 8:-75331031 None:intergenic
CTGCAGGTATCGTTATAGCT+CGG 0.619506 8:+75330404 MsG0880046599.01.T01:intron
GAGAGCACGACCGCGGCCGG+AGG 0.621011 8:-75329605 None:intergenic
CTTCATACCAGAGAAACCTC+CGG 0.628039 8:+75329589 MsG0880046599.01.T01:five_prime_UTR
TTACTGAATTGCCTACCTTG+TGG 0.637396 8:-75331378 None:intergenic
CTGACCAATGAGAAAAGAAG+CGG 0.642004 8:-75329653 None:intergenic
AAGCGGAGAACAGTGAATGA+CGG 0.648242 8:-75329636 None:intergenic
GTTTCTCTGGTATGAAGACG+CGG 0.675552 8:-75329583 None:intergenic
AGAGCTGCTCGATTCATCAA+CGG 0.684223 8:+75330643 MsG0880046599.01.T01:CDS
GCTGCTCGATTCATCAACGG+CGG 0.723347 8:+75330646 MsG0880046599.01.T01:CDS
ACACTAATAGCGACCCAGTG+AGG 0.724106 8:+75330430 MsG0880046599.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTGTTTTTATTTTATTTA+GGG + Chr8:75330972-75330991 MsG0880046599.01.T01:intron 10.0%
!!! TTCTTTTCTTTAATATTTAA+TGG + Chr8:75330467-75330486 MsG0880046599.01.T01:intron 10.0%
!!! TTTCTGTTTTTATTTTATTT+AGG + Chr8:75330971-75330990 MsG0880046599.01.T01:intron 10.0%
!! AATATTAAAGAAAAGAAGTT+TGG - Chr8:75330464-75330483 None:intergenic 15.0%
!! ATAAAAAATTATTGCAATGT+TGG - Chr8:75330574-75330593 None:intergenic 15.0%
!! TAAAAAATTATTGCAATGTT+GGG - Chr8:75330573-75330592 None:intergenic 15.0%
!! TAATTATTTAAGCAAAATCA+TGG - Chr8:75330928-75330947 None:intergenic 15.0%
!!! AATTTTGTATTTAAACTCAA+TGG - Chr8:75329777-75329796 None:intergenic 15.0%
!! AAATTTGTGTTTATCTAAAC+AGG - Chr8:75329866-75329885 None:intergenic 20.0%
!! AATTTGTGTTTATCTAAACA+GGG - Chr8:75329865-75329884 None:intergenic 20.0%
!! AGAAAAAAAACTGAAATTGA+AGG - Chr8:75329690-75329709 None:intergenic 20.0%
!! TATTTGAATGCATATAGTTT+AGG + Chr8:75330120-75330139 MsG0880046599.01.T01:intron 20.0%
!!! ACTTTTAGACAATAACTTAA+TGG + Chr8:75331169-75331188 MsG0880046599.01.T01:intron 20.0%
!!! ATAATAACAATGTTGCTTTA+TGG - Chr8:75330327-75330346 None:intergenic 20.0%
!!! TTTTATTTGTCTAAATTCCA+GGG + Chr8:75330588-75330607 MsG0880046599.01.T01:intron 20.0%
!!! TTTTTATTTGTCTAAATTCC+AGG + Chr8:75330587-75330606 MsG0880046599.01.T01:intron 20.0%
! ACATAATTATGAAAGAGTTC+AGG + Chr8:75331217-75331236 MsG0880046599.01.T01:intron 25.0%
! CAAGTTTATCAATCTATTGA+CGG - Chr8:75331001-75331020 None:intergenic 25.0%
! GAACTCTTTCATAATTATGT+GGG - Chr8:75331217-75331236 None:intergenic 25.0%
! TATAAAACAAAATGAAGACG+AGG - Chr8:75330701-75330720 None:intergenic 25.0%
! TGAACTCTTTCATAATTATG+TGG - Chr8:75331218-75331237 None:intergenic 25.0%
ATCTGATGCAATGAAACTAT+TGG + Chr8:75331355-75331374 MsG0880046599.01.T01:CDS 30.0%
CAGAAATGGAAATCAATTTG+GGG + Chr8:75330195-75330214 MsG0880046599.01.T01:intron 30.0%
GCAGAAATGGAAATCAATTT+GGG + Chr8:75330194-75330213 MsG0880046599.01.T01:intron 30.0%
TAAGCAAAATCATGGCAAAT+GGG - Chr8:75330920-75330939 None:intergenic 30.0%
TGCAGAAATGGAAATCAATT+TGG + Chr8:75330193-75330212 MsG0880046599.01.T01:intron 30.0%
TTAAGCAAAATCATGGCAAA+TGG - Chr8:75330921-75330940 None:intergenic 30.0%
TTATTGCAATGTTGGGTTAA+AGG - Chr8:75330566-75330585 None:intergenic 30.0%
TTTCATAATTATGTGGGTAG+AGG - Chr8:75331211-75331230 None:intergenic 30.0%
! CTGTTATTGATGATTTCATC+TGG + Chr8:75330740-75330759 MsG0880046599.01.T01:intron 30.0%
! CTTCAAGTTCAATAATTGAG+TGG + Chr8:75329717-75329736 MsG0880046599.01.T01:intron 30.0%
! TATTGATGATTTCATCTGGA+TGG + Chr8:75330744-75330763 MsG0880046599.01.T01:intron 30.0%
! TTTGGAATTTATACCTCACT+GGG - Chr8:75330446-75330465 None:intergenic 30.0%
!! AAACGTGAAAGTGTCAAATT+GGG + Chr8:75329994-75330013 MsG0880046599.01.T01:intron 30.0%
!! TAAACGTGAAAGTGTCAAAT+TGG + Chr8:75329993-75330012 MsG0880046599.01.T01:intron 30.0%
!! TATTTTCGAGGGTTTAGTTA+GGG + Chr8:75330034-75330053 MsG0880046599.01.T01:intron 30.0%
!! TTATTTTCGAGGGTTTAGTT+AGG + Chr8:75330033-75330052 MsG0880046599.01.T01:intron 30.0%
!! TTGTAATGTCTGTGTTTTAG+CGG + Chr8:75330787-75330806 MsG0880046599.01.T01:intron 30.0%
!! TTTCATTTGGTGTTAGGTTT+GGG + Chr8:75331262-75331281 MsG0880046599.01.T01:intron 30.0%
!!! TTTTCATTTGGTGTTAGGTT+TGG + Chr8:75331261-75331280 MsG0880046599.01.T01:intron 30.0%
AAGCAAATCTACTCACTCAT+GGG - Chr8:75330891-75330910 None:intergenic 35.0%
CATTTCTGCAAACAACACAT+GGG - Chr8:75330183-75330202 None:intergenic 35.0%
GGAAAACAACTTACAAGTGA+AGG - Chr8:75330289-75330308 None:intergenic 35.0%
GTAATGCACTTTATAGCTCT+TGG + Chr8:75330674-75330693 MsG0880046599.01.T01:CDS 35.0%
GTTTAAGAACCTGAAATGTG+TGG + Chr8:75330056-75330075 MsG0880046599.01.T01:intron 35.0%
TCAGGCAATTATTGTTTCCT+CGG + Chr8:75331235-75331254 MsG0880046599.01.T01:intron 35.0%
TGCTGCTGCATTTATAGTAA+TGG + Chr8:75330362-75330381 MsG0880046599.01.T01:intron 35.0%
TTCATTCTGATCTCATGTGA+AGG - Chr8:75330094-75330113 None:intergenic 35.0%
! AGACGATGATGGTTGTATTA+AGG + Chr8:75331044-75331063 MsG0880046599.01.T01:CDS 35.0%
! ATTATGCTGCTGCTTAATCA+AGG + Chr8:75329839-75329858 MsG0880046599.01.T01:intron 35.0%
! GTTTGGAATTTATACCTCAC+TGG - Chr8:75330447-75330466 None:intergenic 35.0%
!! TTTCCTCGGTTGTTTTCATT+TGG + Chr8:75331249-75331268 MsG0880046599.01.T01:intron 35.0%
!!! AGTTGCACTGTTATTTTCGA+GGG + Chr8:75330023-75330042 MsG0880046599.01.T01:intron 35.0%
!!! GGTTGTTTTCATTTGGTGTT+AGG + Chr8:75331256-75331275 MsG0880046599.01.T01:intron 35.0%
ACACCAAATGAAAACAACCG+AGG - Chr8:75331255-75331274 None:intergenic 40.0%
ACTGAAACGCTCGTGAATAT+CGG + Chr8:75330492-75330511 MsG0880046599.01.T01:intron 40.0%
ATTGCAACAAGAACGTTCTC+TGG - Chr8:75331291-75331310 None:intergenic 40.0%
CAATGAAACTATTGGCCACA+AGG + Chr8:75331363-75331382 MsG0880046599.01.T01:CDS 40.0%
CCATTTCTGCAAACAACACA+TGG - Chr8:75330184-75330203 None:intergenic 40.0%
CTGACCAATGAGAAAAGAAG+CGG - Chr8:75329656-75329675 None:intergenic 40.0%
GAAGCAAATCTACTCACTCA+TGG - Chr8:75330892-75330911 None:intergenic 40.0%
TCTGAAAATCTCGTTGCTAC+AGG + Chr8:75330248-75330267 MsG0880046599.01.T01:CDS 40.0%
TTGTCTAAATTCCAGGGTGT+TGG + Chr8:75330594-75330613 MsG0880046599.01.T01:intron 40.0%
! CAATTCATTTGAAGCTCACG+AGG + Chr8:75331307-75331326 MsG0880046599.01.T01:CDS 40.0%
! CGGGGTTCATTGATAACATT+TGG + Chr8:75331131-75331150 MsG0880046599.01.T01:intron 40.0%
! GTAGCAACGAGATTTTCAGA+CGG - Chr8:75330248-75330267 None:intergenic 40.0%
! TAGCAACGAGATTTTCAGAC+GGG - Chr8:75330247-75330266 None:intergenic 40.0%
! TGTAGCCAGTATCGAGAAAT+CGG - Chr8:75330828-75330847 None:intergenic 40.0%
! TTCTCCGCTTCTTTTCTCAT+TGG + Chr8:75329649-75329668 MsG0880046599.01.T01:five_prime_UTR 40.0%
!! CCATGTGTTGTTTGCAGAAA+TGG + Chr8:75330181-75330200 MsG0880046599.01.T01:intron 40.0%
!!! CAGTTGCACTGTTATTTTCG+AGG + Chr8:75330022-75330041 MsG0880046599.01.T01:intron 40.0%
AAGCGGAGAACAGTGAATGA+CGG - Chr8:75329639-75329658 None:intergenic 45.0%
ACTCATGGGCACTATCAAGT+CGG - Chr8:75330877-75330896 None:intergenic 45.0%
AGAGCTGCTCGATTCATCAA+CGG + Chr8:75330643-75330662 MsG0880046599.01.T01:CDS 45.0%
ATCATCGTCTCCTGAAGCAA+CGG - Chr8:75331034-75331053 None:intergenic 45.0%
CTGAAGTCCAGATTATGCTG+CGG + Chr8:75331111-75331130 MsG0880046599.01.T01:intron 45.0%
CTTCATACCAGAGAAACCTC+CGG + Chr8:75329589-75329608 MsG0880046599.01.T01:five_prime_UTR 45.0%
GTTGCTACAGGACTCATAGA+TGG + Chr8:75330260-75330279 MsG0880046599.01.T01:CDS 45.0%
GTTTCTCTGGTATGAAGACG+CGG - Chr8:75329586-75329605 None:intergenic 45.0%
TGAAGTCCAGATTATGCTGC+GGG + Chr8:75331112-75331131 MsG0880046599.01.T01:intron 45.0%
TGCTTTATGGAAGACTGCGT+GGG - Chr8:75330314-75330333 None:intergenic 45.0%
TTCTCCCGATTTCTCGATAC+TGG + Chr8:75330820-75330839 MsG0880046599.01.T01:intron 45.0%
TTGCTTTATGGAAGACTGCG+TGG - Chr8:75330315-75330334 None:intergenic 45.0%
! CTGCAGGTATCGTTATAGCT+CGG + Chr8:75330404-75330423 MsG0880046599.01.T01:intron 45.0%
! GTAGCCAGTATCGAGAAATC+GGG - Chr8:75330827-75330846 None:intergenic 45.0%
!!! CTGTGTTTTAGCGGTGTTGA+CGG + Chr8:75330796-75330815 MsG0880046599.01.T01:intron 45.0%
!!! TGTGTTTTAGCGGTGTTGAC+GGG + Chr8:75330797-75330816 MsG0880046599.01.T01:intron 45.0%
ACACTAATAGCGACCCAGTG+AGG + Chr8:75330430-75330449 MsG0880046599.01.T01:CDS 50.0%
ACTGAGTCAACCGTTGCTTC+AGG + Chr8:75331021-75331040 MsG0880046599.01.T01:CDS 50.0%
ATGAACCCCGCAGCATAATC+TGG - Chr8:75331121-75331140 None:intergenic 50.0%
CAGCGATGACGATGACAAAC+TGG - Chr8:75330531-75330550 None:intergenic 50.0%
CGAGCTATAACGATACCTGC+AGG - Chr8:75330406-75330425 None:intergenic 50.0%
CTCATGGGCACTATCAAGTC+GGG - Chr8:75330876-75330895 None:intergenic 50.0%
CTGGCTACAGATGTGGAAAC+TGG + Chr8:75330839-75330858 MsG0880046599.01.T01:intron 50.0%
GAAGTCCAGATTATGCTGCG+GGG + Chr8:75331113-75331132 MsG0880046599.01.T01:intron 50.0%
GCTTTATGGAAGACTGCGTG+GGG - Chr8:75330313-75330332 None:intergenic 50.0%
GTTGCTTCAGGAGACGATGA+TGG + Chr8:75331033-75331052 MsG0880046599.01.T01:CDS 50.0%
TAGCGAATCATGTGTCCTGC+AGG + Chr8:75330388-75330407 MsG0880046599.01.T01:intron 50.0%
TATGTGGGTAGAGGTTGCGA+AGG - Chr8:75331202-75331221 None:intergenic 50.0%
! CTCGATACTGGCTACAGATG+TGG + Chr8:75330832-75330851 MsG0880046599.01.T01:intron 50.0%
!! GAACAGTGAATGACGGATGC+CGG - Chr8:75329632-75329651 None:intergenic 50.0%
CAGAGCGTGCCACACATTTC+AGG - Chr8:75330068-75330087 None:intergenic 55.0%
GCTGCTCGATTCATCAACGG+CGG + Chr8:75330646-75330665 MsG0880046599.01.T01:CDS 55.0%
GGCACTATCAAGTCGGGCAA+TGG - Chr8:75330870-75330889 None:intergenic 55.0%
GGCATGAACTTCCAACACCC+TGG - Chr8:75330608-75330627 None:intergenic 55.0%
TCGAGCAGCTCTGCAAGACT+CGG - Chr8:75330635-75330654 None:intergenic 55.0%
TTATGGAAGACTGCGTGGGG+TGG - Chr8:75330310-75330329 None:intergenic 55.0%
!! AGCTCTGCAAGACTCGGTGT+GGG - Chr8:75330629-75330648 None:intergenic 55.0%
CGATTCATCAACGGCGGACG+TGG + Chr8:75330652-75330671 MsG0880046599.01.T01:CDS 60.0%
!! CAGCTCTGCAAGACTCGGTG+TGG - Chr8:75330630-75330649 None:intergenic 60.0%
ACCAGAGAAACCTCCGGCCG+CGG + Chr8:75329595-75329614 MsG0880046599.01.T01:five_prime_UTR 65.0%
ACCGCGGCCGGAGGTTTCTC+TGG - Chr8:75329599-75329618 None:intergenic 70.0%
! TGCCGGAGAGAGCACGACCG+CGG - Chr8:75329615-75329634 None:intergenic 70.0%
GGCCGCGGTCGTGCTCTCTC+CGG + Chr8:75329610-75329629 MsG0880046599.01.T01:five_prime_UTR 75.0%
! GGAGAGAGCACGACCGCGGC+CGG - Chr8:75329611-75329630 None:intergenic 75.0%
!! GAGAGCACGACCGCGGCCGG+AGG - Chr8:75329608-75329627 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr8 gene 75329580 75331388 75329580 ID=MsG0880046599.01;Name=MsG0880046599.01
Chr8 mRNA 75329580 75331388 75329580 ID=MsG0880046599.01.T01;Parent=MsG0880046599.01;Name=MsG0880046599.01.T01;_AED=0.50;_eAED=0.52;_QI=93|0.8|0.66|1|0.6|0.66|6|0|135
Chr8 exon 75329580 75329670 75329580 ID=MsG0880046599.01.T01:exon:31591;Parent=MsG0880046599.01.T01
Chr8 exon 75330198 75330294 75330198 ID=MsG0880046599.01.T01:exon:31592;Parent=MsG0880046599.01.T01
Chr8 exon 75330410 75330451 75330410 ID=MsG0880046599.01.T01:exon:31593;Parent=MsG0880046599.01.T01
Chr8 exon 75330609 75330695 75330609 ID=MsG0880046599.01.T01:exon:31594;Parent=MsG0880046599.01.T01
Chr8 exon 75330993 75331065 75330993 ID=MsG0880046599.01.T01:exon:31595;Parent=MsG0880046599.01.T01
Chr8 exon 75331278 75331388 75331278 ID=MsG0880046599.01.T01:exon:31596;Parent=MsG0880046599.01.T01
Chr8 five_prime_UTR 75329580 75329670 75329580 ID=MsG0880046599.01.T01:five_prime_utr;Parent=MsG0880046599.01.T01
Chr8 five_prime_UTR 75330198 75330199 75330198 ID=MsG0880046599.01.T01:five_prime_utr;Parent=MsG0880046599.01.T01
Chr8 CDS 75330200 75330294 75330200 ID=MsG0880046599.01.T01:cds;Parent=MsG0880046599.01.T01
Chr8 CDS 75330410 75330451 75330410 ID=MsG0880046599.01.T01:cds;Parent=MsG0880046599.01.T01
Chr8 CDS 75330609 75330695 75330609 ID=MsG0880046599.01.T01:cds;Parent=MsG0880046599.01.T01
Chr8 CDS 75330993 75331065 75330993 ID=MsG0880046599.01.T01:cds;Parent=MsG0880046599.01.T01
Chr8 CDS 75331278 75331388 75331278 ID=MsG0880046599.01.T01:cds;Parent=MsG0880046599.01.T01
Gene Sequence

>MsG0880046599.01.T01

ATGGAAATCAATTTGGGGAAACTTGCATTTGACATTGATTTTCACCCGTCTGAAAATCTCGTTGCTACAGGACTCATAGATGGAGACCTTCACTTGTATCGTTATAGCTCGGATAACACTAATAGCGACCCAGTGAGGGTGTTGGAAGTTCATGCCCACACCGAGTCTTGCAGAGCTGCTCGATTCATCAACGGCGGACGTGGTAATGCACTTTATAGCTCTTGGGCTGCCGTCAATAGATTGATAAACTTGACTGAGTCAACCGTTGCTTCAGGAGACGATGATGGTTGTATTAAGGTTTGGGATACCAGAGAACGTTCTTGTTGCAATTCATTTGAAGCTCACGAGGATTACATTTCAGACATTACTTTTGCATCTGATGCAATGAAACTATTGGCCACAAGGTAG

Protein sequence

>MsG0880046599.01.T01

MEINLGKLAFDIDFHPSENLVATGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARFINGGRGNALYSSWAAVNRLINLTESTVASGDDDGCIKVWDTRERSCCNSFEAHEDYISDITFASDAMKLLATR*