AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0100290


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0100290 MtrunA17_Chr1g0149811 96.958 263 7 1 1 263 1 262 0.0 532
Msa0100290 MtrunA17_Chr3g0137111 48.729 236 97 9 23 248 4 225 1.15e-57 183
Msa0100290 MtrunA17_Chr2g0333741 35.563 284 125 13 1 263 1 247 1.62e-31 117
Msa0100290 MtrunA17_Chr4g0001081 37.553 237 96 10 46 263 21 224 6.47e-31 114
Msa0100290 MtrunA17_Chr5g0434121 34.975 203 86 6 31 211 2 180 9.35e-26 101
Msa0100290 MtrunA17_Chr8g0376021 40.690 145 59 4 37 181 15 132 8.23e-25 98.6
Msa0100290 MtrunA17_Chr1g0161901 38.095 147 70 3 35 181 43 168 1.84e-24 98.2
Msa0100290 MtrunA17_Chr3g0110091 54.545 88 22 2 93 180 85 154 5.35e-24 96.7
Msa0100290 MtrunA17_Chr1g0207691 37.594 133 59 3 53 182 21 132 9.96e-19 81.6
Msa0100290 MtrunA17_Chr4g0072571 61.538 52 20 0 91 142 42 93 3.22e-16 74.3
Msa0100290 MtrunA17_Chr3g0081131 30.645 186 97 6 28 198 20 188 6.17e-16 75.9
Msa0100290 MtrunA17_Chr3g0081141 41.176 102 50 3 85 185 89 181 1.75e-14 72.0
Msa0100290 MtrunA17_Chr3g0081121 33.333 186 98 7 28 198 20 194 1.80e-14 71.6
Msa0100290 MtrunA17_Chr5g0396031 38.793 116 56 4 82 195 34 136 1.40e-13 69.7
Msa0100290 MtrunA17_Chr4g0053691 41.111 90 22 2 93 182 66 124 3.07e-13 66.2
Msa0100290 MtrunA17_Chr7g0251581 78.788 33 7 0 94 126 39 71 6.92e-13 65.5
Msa0100290 MtrunA17_Chr7g0254221 70.270 37 11 0 94 130 33 69 9.47e-13 64.3
Msa0100290 MtrunA17_Chr3g0079671 40.000 95 41 4 92 182 96 178 1.22e-11 63.5
Msa0100290 MtrunA17_Chr7g0224221 32.184 174 102 8 27 198 86 245 1.84e-11 63.5
Msa0100290 MtrunA17_Chr2g0310101 35.238 105 53 2 92 195 57 147 3.29e-11 61.2
Msa0100290 MtrunA17_Chr7g0222671 33.333 117 67 5 83 196 55 163 4.93e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0100290 AT5G10970.1 42.616 237 90 9 49 263 60 272 4.17e-44 150
Msa0100290 AT5G25160.1 49.419 172 66 7 51 222 18 168 2.91e-39 137
Msa0100290 AT1G80730.1 37.395 238 127 7 35 263 4 228 1.95e-32 119
Msa0100290 AT1G66140.1 49.515 103 35 3 91 183 80 175 5.80e-22 92.0
Msa0100290 AT1G24625.1 42.727 110 37 4 74 179 33 120 1.25e-16 76.6
Msa0100290 AT5G14010.1 42.697 89 39 3 94 178 36 116 3.86e-14 68.9
Msa0100290 AT5G57520.1 77.778 36 8 0 94 129 50 85 5.08e-14 68.2
Msa0100290 AT1G67030.1 38.938 113 55 3 70 180 13 113 3.39e-13 67.0
Msa0100290 AT3G58070.1 31.638 177 92 7 23 190 23 179 6.47e-12 64.3
Msa0100290 AT5G01860.1 56.250 48 18 1 80 127 54 98 7.43e-11 60.8

Find 32 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAAACTCAAGAATCTTTA+TGG 0.122503 1_3:+6813291 Msa0100290:CDS
ATGGCTTCTTTGCCTCTTTA+TGG 0.168485 1_3:+6813151 Msa0100290:CDS
CTTTGTTTATTATGCATAAT+AGG 0.201649 1_3:-6813118 None:intergenic
CCTTCTCATGTTTCATCTAA+TGG 0.202335 1_3:+6813229 Msa0100290:CDS
CATGTTTCATCTAATGGATT+TGG 0.228057 1_3:+6813235 Msa0100290:CDS
AATGGATTTGGAAACTCTTA+TGG 0.229174 1_3:+6813247 Msa0100290:CDS
ATAGCTTCAGCTTCTGCTTT+TGG 0.233602 1_3:+6813091 Msa0100290:CDS
TTGCCTCTTTATGGTGCTTT+TGG 0.261823 1_3:+6813160 Msa0100290:CDS
ATGAAACATGAGAAGGCTTA+TGG 0.351379 1_3:-6813222 None:intergenic
CTCATAACTTCTTTAGATGT+TGG 0.385754 1_3:+6812899 Msa0100290:CDS
TGCAAAGGTGTGCTTGGACT+TGG 0.428789 1_3:-6812737 None:intergenic
TTCTATAGCTCACAAGCATT+AGG 0.451109 1_3:+6812998 Msa0100290:CDS
TTTGGTGGTAATAAACCTCT+TGG 0.453972 1_3:+6813178 Msa0100290:CDS
AAAGGTGTGCTTGGACTTGG+AGG 0.516654 1_3:-6812734 None:intergenic
TGGAATAGCAAAACTAGGTA+TGG 0.517898 1_3:+6813324 Msa0100290:CDS
ATCTTTATGGATCAACAACC+TGG 0.518646 1_3:+6813304 Msa0100290:CDS
CAACCTGGAATAGCAAAACT+AGG 0.529841 1_3:+6813319 Msa0100290:CDS
CCACCAAAAGCACCATAAAG+AGG 0.536698 1_3:-6813163 None:intergenic
CATCAAGAGGAAGTGTTGGT+AGG 0.548091 1_3:+6813371 Msa0100290:CDS
AAGTGTTGGTAGGTTTGAAG+TGG 0.552808 1_3:+6813381 Msa0100290:CDS
GAAGAAACAACAATCTATTG+AGG 0.558110 1_3:+6812775 Msa0100290:CDS
ATTAATGGAAAGACTAAGCA+AGG 0.563627 1_3:+6812682 None:intergenic
TATGGACATCATCATCATCA+TGG 0.564611 1_3:+6813265 Msa0100290:CDS
TCTTGAGAAGAGAAGATGCA+TGG 0.579539 1_3:-6812707 None:intergenic
GGTAGAAGAAGATATCACTA+AGG 0.583712 1_3:+6812796 Msa0100290:CDS
ACATCATCAAGAGGAAGTGT+TGG 0.585725 1_3:+6813367 Msa0100290:CDS
TATAGCTCACAAGCATTAGG+TGG 0.606344 1_3:+6813001 Msa0100290:CDS
AGAACAACAACATCATCAAG+AGG 0.611847 1_3:+6813358 Msa0100290:CDS
ATATTCATAATCATAGCTTG+AGG 0.612882 1_3:-6813465 None:intergenic
TAAACAAAGTTATGCAAACA+TGG 0.616969 1_3:+6813132 Msa0100290:CDS
CCATTAGATGAAACATGAGA+AGG 0.626125 1_3:-6813229 None:intergenic
GAGTGTGCTTGAATTCCAAG+AGG 0.647089 1_3:-6813193 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTGTTTATTATGCATAAT+AGG - chr1_3:6813121-6813140 None:intergenic 20.0%
!!! CTTTGTTTATTATGCATAAT+AGG - chr1_3:6813121-6813140 None:intergenic 20.0%
!!! TTTTCATGTTGATTTTGCAA+AGG - chr1_3:6812755-6812774 None:intergenic 25.0%
! TAAACAAAGTTATGCAAACA+TGG + chr1_3:6813132-6813151 Msa0100290:CDS 25.0%
!!! TTTTCATGTTGATTTTGCAA+AGG - chr1_3:6812755-6812774 None:intergenic 25.0%
! TAAACAAAGTTATGCAAACA+TGG + chr1_3:6813132-6813151 Msa0100290:CDS 25.0%
GAAGAAACAACAATCTATTG+AGG + chr1_3:6812775-6812794 Msa0100290:CDS 30.0%
CTCATAACTTCTTTAGATGT+TGG + chr1_3:6812899-6812918 Msa0100290:CDS 30.0%
!! CATGTTTCATCTAATGGATT+TGG + chr1_3:6813235-6813254 Msa0100290:CDS 30.0%
! AATGGATTTGGAAACTCTTA+TGG + chr1_3:6813247-6813266 Msa0100290:CDS 30.0%
GTCAAACTCAAGAATCTTTA+TGG + chr1_3:6813291-6813310 Msa0100290:CDS 30.0%
GAAGAAACAACAATCTATTG+AGG + chr1_3:6812775-6812794 Msa0100290:CDS 30.0%
CTCATAACTTCTTTAGATGT+TGG + chr1_3:6812899-6812918 Msa0100290:CDS 30.0%
!! CATGTTTCATCTAATGGATT+TGG + chr1_3:6813235-6813254 Msa0100290:CDS 30.0%
! AATGGATTTGGAAACTCTTA+TGG + chr1_3:6813247-6813266 Msa0100290:CDS 30.0%
GTCAAACTCAAGAATCTTTA+TGG + chr1_3:6813291-6813310 Msa0100290:CDS 30.0%
GGTAGAAGAAGATATCACTA+AGG + chr1_3:6812796-6812815 Msa0100290:CDS 35.0%
TTCTATAGCTCACAAGCATT+AGG + chr1_3:6812998-6813017 Msa0100290:CDS 35.0%
! TCTCTCTTTTGTGAGCATTT+TGG - chr1_3:6813030-6813049 None:intergenic 35.0%
GAGAGATCAATAGCAAAAAG+AGG + chr1_3:6813046-6813065 Msa0100290:CDS 35.0%
GCAAAAAGAGGACAAAGATT+TGG + chr1_3:6813058-6813077 Msa0100290:CDS 35.0%
! TTTGGTGGTAATAAACCTCT+TGG + chr1_3:6813178-6813197 Msa0100290:CDS 35.0%
ATGAAACATGAGAAGGCTTA+TGG - chr1_3:6813225-6813244 None:intergenic 35.0%
CCATTAGATGAAACATGAGA+AGG - chr1_3:6813232-6813251 None:intergenic 35.0%
CCTTCTCATGTTTCATCTAA+TGG + chr1_3:6813229-6813248 Msa0100290:CDS 35.0%
TATGGACATCATCATCATCA+TGG + chr1_3:6813265-6813284 Msa0100290:CDS 35.0%
ATCTTTATGGATCAACAACC+TGG + chr1_3:6813304-6813323 Msa0100290:CDS 35.0%
!!! ATACCTAGTTTTGCTATTCC+AGG - chr1_3:6813325-6813344 None:intergenic 35.0%
TGGAATAGCAAAACTAGGTA+TGG + chr1_3:6813324-6813343 Msa0100290:CDS 35.0%
AGAACAACAACATCATCAAG+AGG + chr1_3:6813358-6813377 Msa0100290:CDS 35.0%
GGTAGAAGAAGATATCACTA+AGG + chr1_3:6812796-6812815 Msa0100290:CDS 35.0%
TTCTATAGCTCACAAGCATT+AGG + chr1_3:6812998-6813017 Msa0100290:CDS 35.0%
! TCTCTCTTTTGTGAGCATTT+TGG - chr1_3:6813030-6813049 None:intergenic 35.0%
GAGAGATCAATAGCAAAAAG+AGG + chr1_3:6813046-6813065 Msa0100290:CDS 35.0%
GCAAAAAGAGGACAAAGATT+TGG + chr1_3:6813058-6813077 Msa0100290:CDS 35.0%
! TTTGGTGGTAATAAACCTCT+TGG + chr1_3:6813178-6813197 Msa0100290:CDS 35.0%
ATGAAACATGAGAAGGCTTA+TGG - chr1_3:6813225-6813244 None:intergenic 35.0%
CCATTAGATGAAACATGAGA+AGG - chr1_3:6813232-6813251 None:intergenic 35.0%
CCTTCTCATGTTTCATCTAA+TGG + chr1_3:6813229-6813248 Msa0100290:CDS 35.0%
TATGGACATCATCATCATCA+TGG + chr1_3:6813265-6813284 Msa0100290:CDS 35.0%
ATCTTTATGGATCAACAACC+TGG + chr1_3:6813304-6813323 Msa0100290:CDS 35.0%
!!! ATACCTAGTTTTGCTATTCC+AGG - chr1_3:6813325-6813344 None:intergenic 35.0%
TGGAATAGCAAAACTAGGTA+TGG + chr1_3:6813324-6813343 Msa0100290:CDS 35.0%
AGAACAACAACATCATCAAG+AGG + chr1_3:6813358-6813377 Msa0100290:CDS 35.0%
TCTTGAGAAGAGAAGATGCA+TGG - chr1_3:6812710-6812729 None:intergenic 40.0%
!! TGATTTTGCAAAGGTGTGCT+TGG - chr1_3:6812746-6812765 None:intergenic 40.0%
TATAGCTCACAAGCATTAGG+TGG + chr1_3:6813001-6813020 Msa0100290:CDS 40.0%
! ATAGCTTCAGCTTCTGCTTT+TGG + chr1_3:6813091-6813110 Msa0100290:CDS 40.0%
ATGGCTTCTTTGCCTCTTTA+TGG + chr1_3:6813151-6813170 Msa0100290:CDS 40.0%
! TTGCCTCTTTATGGTGCTTT+TGG + chr1_3:6813160-6813179 Msa0100290:CDS 40.0%
GACATCATCATCATCATGGT+TGG + chr1_3:6813269-6813288 Msa0100290:CDS 40.0%
CAACCTGGAATAGCAAAACT+AGG + chr1_3:6813319-6813338 Msa0100290:CDS 40.0%
! ACATCATCAAGAGGAAGTGT+TGG + chr1_3:6813367-6813386 Msa0100290:CDS 40.0%
!! AAGTGTTGGTAGGTTTGAAG+TGG + chr1_3:6813381-6813400 Msa0100290:CDS 40.0%
TCTTGAGAAGAGAAGATGCA+TGG - chr1_3:6812710-6812729 None:intergenic 40.0%
!! TGATTTTGCAAAGGTGTGCT+TGG - chr1_3:6812746-6812765 None:intergenic 40.0%
TATAGCTCACAAGCATTAGG+TGG + chr1_3:6813001-6813020 Msa0100290:CDS 40.0%
! ATAGCTTCAGCTTCTGCTTT+TGG + chr1_3:6813091-6813110 Msa0100290:CDS 40.0%
ATGGCTTCTTTGCCTCTTTA+TGG + chr1_3:6813151-6813170 Msa0100290:CDS 40.0%
! TTGCCTCTTTATGGTGCTTT+TGG + chr1_3:6813160-6813179 Msa0100290:CDS 40.0%
GACATCATCATCATCATGGT+TGG + chr1_3:6813269-6813288 Msa0100290:CDS 40.0%
CAACCTGGAATAGCAAAACT+AGG + chr1_3:6813319-6813338 Msa0100290:CDS 40.0%
! ACATCATCAAGAGGAAGTGT+TGG + chr1_3:6813367-6813386 Msa0100290:CDS 40.0%
!! AAGTGTTGGTAGGTTTGAAG+TGG + chr1_3:6813381-6813400 Msa0100290:CDS 40.0%
!! CCACCAAAAGCACCATAAAG+AGG - chr1_3:6813166-6813185 None:intergenic 45.0%
! CCTCTTTATGGTGCTTTTGG+TGG + chr1_3:6813163-6813182 Msa0100290:CDS 45.0%
GAGTGTGCTTGAATTCCAAG+AGG - chr1_3:6813196-6813215 None:intergenic 45.0%
!! CATCAAGAGGAAGTGTTGGT+AGG + chr1_3:6813371-6813390 Msa0100290:CDS 45.0%
!! CCACCAAAAGCACCATAAAG+AGG - chr1_3:6813166-6813185 None:intergenic 45.0%
! CCTCTTTATGGTGCTTTTGG+TGG + chr1_3:6813163-6813182 Msa0100290:CDS 45.0%
GAGTGTGCTTGAATTCCAAG+AGG - chr1_3:6813196-6813215 None:intergenic 45.0%
!! CATCAAGAGGAAGTGTTGGT+AGG + chr1_3:6813371-6813390 Msa0100290:CDS 45.0%
! AAAGGTGTGCTTGGACTTGG+AGG - chr1_3:6812737-6812756 None:intergenic 50.0%
! TGCAAAGGTGTGCTTGGACT+TGG - chr1_3:6812740-6812759 None:intergenic 50.0%
! AAAGGTGTGCTTGGACTTGG+AGG - chr1_3:6812737-6812756 None:intergenic 50.0%
! TGCAAAGGTGTGCTTGGACT+TGG - chr1_3:6812740-6812759 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 6812686 6813477 6812686 ID=Msa0100290;Name=Msa0100290
chr1_3 mRNA 6812686 6813477 6812686 ID=Msa0100290-mRNA-1;Parent=Msa0100290;Name=Msa0100290-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|263
chr1_3 exon 6812686 6813477 6812686 ID=Msa0100290-mRNA-1:exon:2585;Parent=Msa0100290-mRNA-1
chr1_3 CDS 6812686 6813477 6812686 ID=Msa0100290-mRNA-1:cds;Parent=Msa0100290-mRNA-1
Gene Sequence

>Msa0100290

ATGGAAAGACTAAGCAAGGAACCATGCATCTTCTCTTCTCAAGAAACTCCTCCAAGTCCAAGCACACCTTTGCAAAATCAACATGAAAAGAAGAAACAACAATCTATTGAGGTAGAAGAAGATATCACTAAGGTAGAAACTTTATTTGATCTAAATGTATCTAGCAATGATTCTAATCTTGATTGTAATAGTCAGCAAGAAACAGAACTCAATCTCATAACTTCTTTAGATGTTGGTAATTCATCATCACAAAACAATAATACTCTTGATGTAGAACAAAGAATTTTTTCTTGTAACTATTGTCATAGAAAATTCTATAGCTCACAAGCATTAGGTGGACACCAAAATGCTCACAAAAGAGAGAGATCAATAGCAAAAAGAGGACAAAGATTTGGAACACAAATAATAGCTTCAGCTTCTGCTTTTGGATTTCCTATTATGCATAATAAACAAAGTTATGCAAACATGGCTTCTTTGCCTCTTTATGGTGCTTTTGGTGGTAATAAACCTCTTGGAATTCAAGCACACTCTATGATCCATAAGCCTTCTCATGTTTCATCTAATGGATTTGGAAACTCTTATGGACATCATCATCATCATGGTTGGTCAAACTCAAGAATCTTTATGGATCAACAACCTGGAATAGCAAAACTAGGTATGGCTGATTTTCATAGAACAACAACATCATCAAGAGGAAGTGTTGGTAGGTTTGAAGTGGTAGATACTATGATTAATTCTGCAGCTAATAAAGAAATGAAGCAGAAGCAGCTTGATTTAAACCTCAAGCTATGA

Protein sequence

>Msa0100290

MERLSKEPCIFSSQETPPSPSTPLQNQHEKKKQQSIEVEEDITKVETLFDLNVSSNDSNLDCNSQQETELNLITSLDVGNSSSQNNNTLDVEQRIFSCNYCHRKFYSSQALGGHQNAHKRERSIAKRGQRFGTQIIASASAFGFPIMHNKQSYANMASLPLYGAFGGNKPLGIQAHSMIHKPSHVSSNGFGNSYGHHHHHGWSNSRIFMDQQPGIAKLGMADFHRTTTSSRGSVGRFEVVDTMINSAANKEMKQKQLDLNLKL*