AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0054920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054920 MtrunA17_Chr1g0149811 95.312 128 5 1 78 205 136 262 2.88e-88 260
Msa0054920 MtrunA17_Chr1g0149811 95.062 81 4 0 1 81 1 81 1.99e-48 159
Msa0054920 MtrunA17_Chr3g0137111 34.061 229 83 9 23 190 4 225 9.05e-20 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054920 AT5G10970.1 37.333 150 56 7 78 205 139 272 1.23e-13 68.2

Find 29 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCAAACTCAAGAATCTTTA+TGG 0.122503 1_2:+9694262 Msa0054920:CDS
ATGGCTTCTTTGCCTCTTTA+TGG 0.168485 1_2:+9694122 Msa0054920:CDS
CTTTGTTTATTATGCATAAT+AGG 0.201649 1_2:-9694089 None:intergenic
CCTTCTCATGTTTCATCTAA+TGG 0.202335 1_2:+9694200 Msa0054920:CDS
CATGTTTCATCTAATGGATT+TGG 0.228057 1_2:+9694206 Msa0054920:CDS
AATGGATTTGGAAACTCTTA+TGG 0.229174 1_2:+9694218 Msa0054920:CDS
ATAGCTTCAGCTTCTGCTTT+TGG 0.233602 1_2:+9694062 Msa0054920:intron
TTGCCTCTTTATGGTGCTTT+TGG 0.261823 1_2:+9694131 Msa0054920:CDS
ATGAAACATGAGAAGGCTTA+TGG 0.351379 1_2:-9694193 None:intergenic
CTCATAACTTCTTTAGATGT+TGG 0.385754 1_2:+9693871 Msa0054920:CDS
TGCAAAGGTGTGCTTGGACT+TGG 0.428789 1_2:-9693709 None:intergenic
TTTGGTGGTAATAAACCTCT+TGG 0.453972 1_2:+9694149 Msa0054920:CDS
AAAGGTGTGCTTGGACTTGG+AGG 0.516654 1_2:-9693706 None:intergenic
TGGAATAGCAAAACTAGGTA+TGG 0.517898 1_2:+9694295 Msa0054920:CDS
ATCTTTATGGATCAACAACC+TGG 0.518646 1_2:+9694275 Msa0054920:CDS
CAACCTGGAATAGCAAAACT+AGG 0.529841 1_2:+9694290 Msa0054920:CDS
CCACCAAAAGCACCATAAAG+AGG 0.536698 1_2:-9694134 None:intergenic
CATCAAGAGGAAGTGTTGGT+AGG 0.548091 1_2:+9694342 Msa0054920:CDS
GAAGAAACAACAATCTATTG+AGG 0.558110 1_2:+9693747 Msa0054920:CDS
ATTAATGGAAAGACTAAGCA+AGG 0.563627 1_2:+9693654 None:intergenic
TATGGACATCATCATCATCA+TGG 0.564611 1_2:+9694236 Msa0054920:CDS
TCTTGAGAAGAGAAGATGCA+TGG 0.579539 1_2:-9693679 None:intergenic
GGTAGAAGAAGATATCACTA+AGG 0.583712 1_2:+9693768 Msa0054920:CDS
ACATCATCAAGAGGAAGTGT+TGG 0.585725 1_2:+9694338 Msa0054920:CDS
AGAACAACAACATCATCAAG+AGG 0.611847 1_2:+9694329 Msa0054920:CDS
ATATTCATAATCATAGCTTG+AGG 0.612882 1_2:-9694436 None:intergenic
TAAACAAAGTTATGCAAACA+TGG 0.616969 1_2:+9694103 Msa0054920:CDS
CCATTAGATGAAACATGAGA+AGG 0.626125 1_2:-9694200 None:intergenic
GAGTGTGCTTGAATTCCAAG+AGG 0.647089 1_2:-9694164 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTGTTTATTATGCATAAT+AGG - chr1_2:9694092-9694111 None:intergenic 20.0%
!!! CTTTGTTTATTATGCATAAT+AGG - chr1_2:9694092-9694111 None:intergenic 20.0%
!!! TTTTCATGTTGATTTTGCAA+AGG - chr1_2:9693727-9693746 None:intergenic 25.0%
! TAAACAAAGTTATGCAAACA+TGG + chr1_2:9694103-9694122 Msa0054920:CDS 25.0%
!!! TTTTCATGTTGATTTTGCAA+AGG - chr1_2:9693727-9693746 None:intergenic 25.0%
! TAAACAAAGTTATGCAAACA+TGG + chr1_2:9694103-9694122 Msa0054920:CDS 25.0%
GAAGAAACAACAATCTATTG+AGG + chr1_2:9693747-9693766 Msa0054920:CDS 30.0%
CTCATAACTTCTTTAGATGT+TGG + chr1_2:9693871-9693890 Msa0054920:CDS 30.0%
!! CATGTTTCATCTAATGGATT+TGG + chr1_2:9694206-9694225 Msa0054920:CDS 30.0%
! AATGGATTTGGAAACTCTTA+TGG + chr1_2:9694218-9694237 Msa0054920:CDS 30.0%
GTCAAACTCAAGAATCTTTA+TGG + chr1_2:9694262-9694281 Msa0054920:CDS 30.0%
GAAGAAACAACAATCTATTG+AGG + chr1_2:9693747-9693766 Msa0054920:CDS 30.0%
CTCATAACTTCTTTAGATGT+TGG + chr1_2:9693871-9693890 Msa0054920:CDS 30.0%
!! CATGTTTCATCTAATGGATT+TGG + chr1_2:9694206-9694225 Msa0054920:CDS 30.0%
! AATGGATTTGGAAACTCTTA+TGG + chr1_2:9694218-9694237 Msa0054920:CDS 30.0%
GTCAAACTCAAGAATCTTTA+TGG + chr1_2:9694262-9694281 Msa0054920:CDS 30.0%
GGTAGAAGAAGATATCACTA+AGG + chr1_2:9693768-9693787 Msa0054920:CDS 35.0%
TTCTATAGCTCACAAGCATT+AGG + chr1_2:9693970-9693989 Msa0054920:intron 35.0%
! TCTCTCTTTTGTGAGCATTT+TGG - chr1_2:9694002-9694021 None:intergenic 35.0%
GAGAGATCAATAGCAAAAAG+AGG + chr1_2:9694018-9694037 Msa0054920:intron 35.0%
GCAAAAAGAGGACAAAGATT+TGG + chr1_2:9694030-9694049 Msa0054920:intron 35.0%
! TTTGGTGGTAATAAACCTCT+TGG + chr1_2:9694149-9694168 Msa0054920:CDS 35.0%
ATGAAACATGAGAAGGCTTA+TGG - chr1_2:9694196-9694215 None:intergenic 35.0%
CCATTAGATGAAACATGAGA+AGG - chr1_2:9694203-9694222 None:intergenic 35.0%
CCTTCTCATGTTTCATCTAA+TGG + chr1_2:9694200-9694219 Msa0054920:CDS 35.0%
TATGGACATCATCATCATCA+TGG + chr1_2:9694236-9694255 Msa0054920:CDS 35.0%
ATCTTTATGGATCAACAACC+TGG + chr1_2:9694275-9694294 Msa0054920:CDS 35.0%
!!! ATACCTAGTTTTGCTATTCC+AGG - chr1_2:9694296-9694315 None:intergenic 35.0%
TGGAATAGCAAAACTAGGTA+TGG + chr1_2:9694295-9694314 Msa0054920:CDS 35.0%
AGAACAACAACATCATCAAG+AGG + chr1_2:9694329-9694348 Msa0054920:CDS 35.0%
GGTAGAAGAAGATATCACTA+AGG + chr1_2:9693768-9693787 Msa0054920:CDS 35.0%
TTCTATAGCTCACAAGCATT+AGG + chr1_2:9693970-9693989 Msa0054920:intron 35.0%
! TCTCTCTTTTGTGAGCATTT+TGG - chr1_2:9694002-9694021 None:intergenic 35.0%
GAGAGATCAATAGCAAAAAG+AGG + chr1_2:9694018-9694037 Msa0054920:intron 35.0%
GCAAAAAGAGGACAAAGATT+TGG + chr1_2:9694030-9694049 Msa0054920:intron 35.0%
! TTTGGTGGTAATAAACCTCT+TGG + chr1_2:9694149-9694168 Msa0054920:CDS 35.0%
ATGAAACATGAGAAGGCTTA+TGG - chr1_2:9694196-9694215 None:intergenic 35.0%
CCATTAGATGAAACATGAGA+AGG - chr1_2:9694203-9694222 None:intergenic 35.0%
CCTTCTCATGTTTCATCTAA+TGG + chr1_2:9694200-9694219 Msa0054920:CDS 35.0%
TATGGACATCATCATCATCA+TGG + chr1_2:9694236-9694255 Msa0054920:CDS 35.0%
ATCTTTATGGATCAACAACC+TGG + chr1_2:9694275-9694294 Msa0054920:CDS 35.0%
!!! ATACCTAGTTTTGCTATTCC+AGG - chr1_2:9694296-9694315 None:intergenic 35.0%
TGGAATAGCAAAACTAGGTA+TGG + chr1_2:9694295-9694314 Msa0054920:CDS 35.0%
AGAACAACAACATCATCAAG+AGG + chr1_2:9694329-9694348 Msa0054920:CDS 35.0%
TCTTGAGAAGAGAAGATGCA+TGG - chr1_2:9693682-9693701 None:intergenic 40.0%
!! TGATTTTGCAAAGGTGTGCT+TGG - chr1_2:9693718-9693737 None:intergenic 40.0%
TATAGCTCACAAGCATTAGG+TGG + chr1_2:9693973-9693992 Msa0054920:intron 40.0%
! ATAGCTTCAGCTTCTGCTTT+TGG + chr1_2:9694062-9694081 Msa0054920:intron 40.0%
ATGGCTTCTTTGCCTCTTTA+TGG + chr1_2:9694122-9694141 Msa0054920:CDS 40.0%
! TTGCCTCTTTATGGTGCTTT+TGG + chr1_2:9694131-9694150 Msa0054920:CDS 40.0%
GACATCATCATCATCATGGT+TGG + chr1_2:9694240-9694259 Msa0054920:CDS 40.0%
CAACCTGGAATAGCAAAACT+AGG + chr1_2:9694290-9694309 Msa0054920:CDS 40.0%
! ACATCATCAAGAGGAAGTGT+TGG + chr1_2:9694338-9694357 Msa0054920:CDS 40.0%
TCTTGAGAAGAGAAGATGCA+TGG - chr1_2:9693682-9693701 None:intergenic 40.0%
!! TGATTTTGCAAAGGTGTGCT+TGG - chr1_2:9693718-9693737 None:intergenic 40.0%
TATAGCTCACAAGCATTAGG+TGG + chr1_2:9693973-9693992 Msa0054920:intron 40.0%
! ATAGCTTCAGCTTCTGCTTT+TGG + chr1_2:9694062-9694081 Msa0054920:intron 40.0%
ATGGCTTCTTTGCCTCTTTA+TGG + chr1_2:9694122-9694141 Msa0054920:CDS 40.0%
! TTGCCTCTTTATGGTGCTTT+TGG + chr1_2:9694131-9694150 Msa0054920:CDS 40.0%
GACATCATCATCATCATGGT+TGG + chr1_2:9694240-9694259 Msa0054920:CDS 40.0%
CAACCTGGAATAGCAAAACT+AGG + chr1_2:9694290-9694309 Msa0054920:CDS 40.0%
! ACATCATCAAGAGGAAGTGT+TGG + chr1_2:9694338-9694357 Msa0054920:CDS 40.0%
!! CCACCAAAAGCACCATAAAG+AGG - chr1_2:9694137-9694156 None:intergenic 45.0%
! CCTCTTTATGGTGCTTTTGG+TGG + chr1_2:9694134-9694153 Msa0054920:CDS 45.0%
GAGTGTGCTTGAATTCCAAG+AGG - chr1_2:9694167-9694186 None:intergenic 45.0%
!! CATCAAGAGGAAGTGTTGGT+AGG + chr1_2:9694342-9694361 Msa0054920:CDS 45.0%
!! CCACCAAAAGCACCATAAAG+AGG - chr1_2:9694137-9694156 None:intergenic 45.0%
! CCTCTTTATGGTGCTTTTGG+TGG + chr1_2:9694134-9694153 Msa0054920:CDS 45.0%
GAGTGTGCTTGAATTCCAAG+AGG - chr1_2:9694167-9694186 None:intergenic 45.0%
!! CATCAAGAGGAAGTGTTGGT+AGG + chr1_2:9694342-9694361 Msa0054920:CDS 45.0%
! AAAGGTGTGCTTGGACTTGG+AGG - chr1_2:9693709-9693728 None:intergenic 50.0%
! TGCAAAGGTGTGCTTGGACT+TGG - chr1_2:9693712-9693731 None:intergenic 50.0%
! AAAGGTGTGCTTGGACTTGG+AGG - chr1_2:9693709-9693728 None:intergenic 50.0%
! TGCAAAGGTGTGCTTGGACT+TGG - chr1_2:9693712-9693731 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 9693658 9694448 9693658 ID=Msa0054920;Name=Msa0054920
chr1_2 mRNA 9693658 9694448 9693658 ID=Msa0054920-mRNA-1;Parent=Msa0054920;Name=Msa0054920-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0|0|1|1|1|2|0|205
chr1_2 exon 9693658 9693892 9693658 ID=Msa0054920-mRNA-1:exon:3620;Parent=Msa0054920-mRNA-1
chr1_2 exon 9694066 9694448 9694066 ID=Msa0054920-mRNA-1:exon:3621;Parent=Msa0054920-mRNA-1
chr1_2 CDS 9693658 9693892 9693658 ID=Msa0054920-mRNA-1:cds;Parent=Msa0054920-mRNA-1
chr1_2 CDS 9694066 9694448 9694066 ID=Msa0054920-mRNA-1:cds;Parent=Msa0054920-mRNA-1
Gene Sequence

>Msa0054920

ATGGAAAGACTAAGCAAGGAACCATGCATCTTCTCTTCTCAAGAAACTCCTCCAAGTCCAAGCACACCTTTGCAAAATCAACATGAAAAGAAGAAACAACAATCTATTGAGGTAGAAGAAGATATCACTAAGGTAGAAACTTTATTTGATCTAAATGTATCTAGCAATGATTCTAATCTTGATTGTAATAGTCAGCAAGAAACAGAACTCAATCTCATAACTTCTTTAGATGTTGCTTCAGCTTCTGCTTTTGGATTTCCTATTATGCATAATAAACAAAGTTATGCAAACATGGCTTCTTTGCCTCTTTATGGTGCTTTTGGTGGTAATAAACCTCTTGGAATTCAAGCACACTCTATGATCCATAAGCCTTCTCATGTTTCATCTAATGGATTTGGAAACTCTTATGGACATCATCATCATCATGGTTGGTCAAACTCAAGAATCTTTATGGATCAACAACCTGGAATAGCAAAACTAGGTATGGCTGATTTTCATAGAACAACAACATCATCAAGAGGAAGTGTTGGTAGGTTTGAAGTCGTAGATACTATGATTAATTCTGCAGCTAATAAAGAAATGAAGCAGAAGCAGCTTGATTTAAACCTCAAGCTATGA

Protein sequence

>Msa0054920

MERLSKEPCIFSSQETPPSPSTPLQNQHEKKKQQSIEVEEDITKVETLFDLNVSSNDSNLDCNSQQETELNLITSLDVASASAFGFPIMHNKQSYANMASLPLYGAFGGNKPLGIQAHSMIHKPSHVSSNGFGNSYGHHHHHGWSNSRIFMDQQPGIAKLGMADFHRTTTSSRGSVGRFEVVDTMINSAANKEMKQKQLDLNLKL*