Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1359400 | XP_013465831.1 | 96.958 | 263 | 7 | 1 | 1 | 263 | 1 | 262 | 0.0 | 532 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1359400 | sp|Q39262|ZFP3_ARATH | 49.419 | 172 | 66 | 7 | 51 | 222 | 18 | 168 | 2.87e-38 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1359400 | A0A072VDM2 | 96.958 | 263 | 7 | 1 | 1 | 263 | 1 | 262 | 0.0 | 532 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001550 | Msa1359400 | -0.822742 | 2.013160e-53 | -8.615850e-47 |
| Msa0010110 | Msa1359400 | 0.802626 | 5.132270e-49 | -8.615850e-47 |
| Msa0023480 | Msa1359400 | 0.825603 | 4.283741e-54 | -8.615850e-47 |
| Msa0046110 | Msa1359400 | -0.813907 | 2.017783e-51 | -8.615850e-47 |
| Msa0054920 | Msa1359400 | 0.957605 | 1.812925e-115 | -8.615850e-47 |
| Msa0071250 | Msa1359400 | 0.822646 | 2.119177e-53 | -8.615850e-47 |
| Msa0090510 | Msa1359400 | 0.815289 | 9.976175e-52 | -8.615850e-47 |
| Msa0100290 | Msa1359400 | 0.988760 | 2.680613e-175 | -8.615850e-47 |
| Msa0104670 | Msa1359400 | 0.806084 | 9.764654e-50 | -8.615850e-47 |
| Msa0117520 | Msa1359400 | 0.804593 | 2.005467e-49 | -8.615850e-47 |
| Msa1346840 | Msa1359400 | 0.842538 | 2.431939e-58 | -8.615850e-47 |
| Msa1359400 | Msa1363820 | -0.809608 | 1.740046e-50 | -8.615850e-47 |
| Msa1359400 | Msa1368360 | 0.860003 | 2.795804e-63 | -8.615850e-47 |
| Msa1359400 | Msa1369920 | -0.844132 | 9.134197e-59 | -8.615850e-47 |
| Msa1359400 | Msa1375610 | -0.819981 | 8.729050e-53 | -8.615850e-47 |
| Msa1359400 | Msa1376550 | -0.824682 | 7.070796e-54 | -8.615850e-47 |
| Msa1359400 | Msa1383740 | 0.803040 | 4.214734e-49 | -8.615850e-47 |
| Msa1359400 | Msa1383960 | 0.803038 | 4.217264e-49 | -8.615850e-47 |
| Msa1359400 | Msa1385720 | 0.810233 | 1.276457e-50 | -8.615850e-47 |
| Msa1359400 | Msa1407190 | 0.802687 | 4.983647e-49 | -8.615850e-47 |
| Msa1359400 | Msa1412130 | 0.847119 | 1.414577e-59 | -8.615850e-47 |
| Msa1359400 | Msa1423540 | 0.811933 | 5.464010e-51 | -8.615850e-47 |
| Msa1359400 | Msa1425260 | 0.830318 | 3.141753e-55 | -8.615850e-47 |
| Msa1359400 | Msa1430550 | 0.800149 | 1.650207e-48 | -8.615850e-47 |
| Msa1359400 | Msa1434090 | 0.814253 | 1.692513e-51 | -8.615850e-47 |
| Msa0641130 | Msa1359400 | 0.816289 | 5.972076e-52 | -8.615850e-47 |
| Msa0672550 | Msa1359400 | 0.800908 | 1.155829e-48 | -8.615850e-47 |
| Msa0705990 | Msa1359400 | 0.801304 | 9.590286e-49 | -8.615850e-47 |
| Msa0733010 | Msa1359400 | 0.803487 | 3.406186e-49 | -8.615850e-47 |
| Msa0738030 | Msa1359400 | 0.814703 | 1.346084e-51 | -8.615850e-47 |
| Msa0741440 | Msa1359400 | 0.804468 | 2.129568e-49 | -8.615850e-47 |
| Msa0754820 | Msa1359400 | 0.813876 | 2.049654e-51 | -8.615850e-47 |
| Msa0771950 | Msa1359400 | 0.837027 | 6.622981e-57 | -8.615850e-47 |
| Msa0772280 | Msa1359400 | 0.840913 | 6.530971e-58 | -8.615850e-47 |
| Msa0810440 | Msa1359400 | 0.816136 | 6.461394e-52 | -8.615850e-47 |
| Msa0822500 | Msa1359400 | 0.805229 | 1.476510e-49 | -8.615850e-47 |
| Msa0823540 | Msa1359400 | 0.830769 | 2.436815e-55 | -8.615850e-47 |
| Msa0127020 | Msa1359400 | 0.801195 | 1.009719e-48 | -8.615850e-47 |
| Msa0138020 | Msa1359400 | 0.817853 | 2.658561e-52 | -8.615850e-47 |
| Msa0144530 | Msa1359400 | 0.937933 | 1.545271e-98 | -8.615850e-47 |
| Msa0148080 | Msa1359400 | 0.804502 | 2.094426e-49 | -8.615850e-47 |
| Msa0150860 | Msa1359400 | 0.807685 | 4.480243e-50 | -8.615850e-47 |
| Msa0160960 | Msa1359400 | 0.821825 | 3.286634e-53 | -8.615850e-47 |
| Msa0169280 | Msa1359400 | 0.802071 | 6.676324e-49 | -8.615850e-47 |
| Msa0183910 | Msa1359400 | 0.803512 | 3.365445e-49 | -8.615850e-47 |
| Msa0206830 | Msa1359400 | 0.800332 | 1.514383e-48 | -8.615850e-47 |
| Msa0217700 | Msa1359400 | 0.809392 | 1.935740e-50 | -8.615850e-47 |
| Msa0220400 | Msa1359400 | -0.801426 | 9.055855e-49 | -8.615850e-47 |
| Msa1225860 | Msa1359400 | -0.808364 | 3.212466e-50 | -8.615850e-47 |
| Msa1229480 | Msa1359400 | 0.803109 | 4.077190e-49 | -8.615850e-47 |
| Msa1236110 | Msa1359400 | -0.827488 | 1.522047e-54 | -8.615850e-47 |
| Msa1237700 | Msa1359400 | 0.812412 | 4.295822e-51 | -8.615850e-47 |
| Msa1272010 | Msa1359400 | 0.829378 | 5.322397e-55 | -8.615850e-47 |
| Msa1277410 | Msa1359400 | 0.822318 | 2.526177e-53 | -8.615850e-47 |
| Msa1298690 | Msa1359400 | 0.820062 | 8.366258e-53 | -8.615850e-47 |
| Msa1303070 | Msa1359400 | 0.805762 | 1.141370e-49 | -8.615850e-47 |
| Msa1307160 | Msa1359400 | 0.824169 | 9.335645e-54 | -8.615850e-47 |
| Msa1307180 | Msa1359400 | 0.845662 | 3.532059e-59 | -8.615850e-47 |
| Msa1309760 | Msa1359400 | 0.847202 | 1.342189e-59 | -8.615850e-47 |
| Msa1316360 | Msa1359400 | 0.848607 | 5.502591e-60 | -8.615850e-47 |
| Msa1325410 | Msa1359400 | -0.816822 | 4.536262e-52 | -8.615850e-47 |
| Msa1330540 | Msa1359400 | -0.813983 | 1.941785e-51 | -8.615850e-47 |
| Msa1340200 | Msa1359400 | 0.805189 | 1.505088e-49 | -8.615850e-47 |
| Msa0366210 | Msa1359400 | 0.802444 | 5.594954e-49 | -8.615850e-47 |
| Msa0374340 | Msa1359400 | -0.818051 | 2.398504e-52 | -8.615850e-47 |
| Msa0374420 | Msa1359400 | -0.803935 | 2.749579e-49 | -8.615850e-47 |
| Msa0380400 | Msa1359400 | -0.812007 | 5.265588e-51 | -8.615850e-47 |
| Msa0386940 | Msa1359400 | 0.826373 | 2.811325e-54 | -8.615850e-47 |
| Msa0403470 | Msa1359400 | -0.802203 | 6.271825e-49 | -8.615850e-47 |
| Msa0403480 | Msa1359400 | -0.802452 | 5.573594e-49 | -8.615850e-47 |
| Msa0403500 | Msa1359400 | 0.803051 | 4.192568e-49 | -8.615850e-47 |
| Msa0427580 | Msa1359400 | 0.812886 | 3.382372e-51 | -8.615850e-47 |
| Msa0428200 | Msa1359400 | 0.802319 | 5.936609e-49 | -8.615850e-47 |
| Msa0430060 | Msa1359400 | 0.809483 | 1.850793e-50 | -8.615850e-47 |
| Msa0430150 | Msa1359400 | 0.810943 | 8.961835e-51 | -8.615850e-47 |
| Msa0476170 | Msa1359400 | -0.827038 | 1.950811e-54 | -8.615850e-47 |
| Msa0481030 | Msa1359400 | 0.820847 | 5.525094e-53 | -8.615850e-47 |
| Msa0992580 | Msa1359400 | 0.801040 | 1.086056e-48 | -8.615850e-47 |
| Msa1023310 | Msa1359400 | 0.808588 | 2.877107e-50 | -8.615850e-47 |
| Msa1024680 | Msa1359400 | 0.800608 | 1.330435e-48 | -8.615850e-47 |
| Msa1062040 | Msa1359400 | 0.806856 | 6.712028e-50 | -8.615850e-47 |
| Msa1070540 | Msa1359400 | 0.806063 | 9.864420e-50 | -8.615850e-47 |
| Msa1085610 | Msa1359400 | -0.813650 | 2.298829e-51 | -8.615850e-47 |
| Msa1089460 | Msa1359400 | 0.813311 | 2.729668e-51 | -8.615850e-47 |
| Msa1101240 | Msa1359400 | 0.802810 | 4.701334e-49 | -8.615850e-47 |
| Msa0241140 | Msa1359400 | 0.840061 | 1.090671e-57 | -8.615850e-47 |
| Msa0269560 | Msa1359400 | 0.802618 | 5.150337e-49 | -8.615850e-47 |
| Msa0278580 | Msa1359400 | 0.816498 | 5.361777e-52 | -8.615850e-47 |
| Msa0295460 | Msa1359400 | -0.823420 | 1.398657e-53 | -8.615850e-47 |
| Msa0296050 | Msa1359400 | -0.847392 | 1.190441e-59 | -8.615850e-47 |
| Msa0302840 | Msa1359400 | 0.804861 | 1.762862e-49 | -8.615850e-47 |
| Msa0317960 | Msa1359400 | 0.807951 | 3.932699e-50 | -8.615850e-47 |
| Msa0317970 | Msa1359400 | 0.804349 | 2.254828e-49 | -8.615850e-47 |
| Msa0318680 | Msa1359400 | 0.800220 | 1.596073e-48 | -8.615850e-47 |
| Msa0333850 | Msa1359400 | -0.828299 | 9.712888e-55 | -8.615850e-47 |
| Msa0333970 | Msa1359400 | -0.841060 | 5.973836e-58 | -8.615850e-47 |
| Msa0339910 | Msa1359400 | 0.820834 | 5.563954e-53 | -8.615850e-47 |
| Msa1107200 | Msa1359400 | 0.817641 | 2.968054e-52 | -8.615850e-47 |
| Msa1108710 | Msa1359400 | 0.801006 | 1.103606e-48 | -8.615850e-47 |
| Msa1123770 | Msa1359400 | 0.807024 | 6.186548e-50 | -8.615850e-47 |
| Msa1144040 | Msa1359400 | 0.801715 | 7.899928e-49 | -8.615850e-47 |
| Msa1150780 | Msa1359400 | 0.818096 | 2.342780e-52 | -8.615850e-47 |
| Msa1158390 | Msa1359400 | 0.828892 | 6.983776e-55 | -8.615850e-47 |
| Msa1160560 | Msa1359400 | 0.805238 | 1.470243e-49 | -8.615850e-47 |
| Msa1169910 | Msa1359400 | 0.809473 | 1.859461e-50 | -8.615850e-47 |
| Msa1171140 | Msa1359400 | 0.806147 | 9.472370e-50 | -8.615850e-47 |
| Msa1176170 | Msa1359400 | 0.827152 | 1.831343e-54 | -8.615850e-47 |
| Msa1179070 | Msa1359400 | 0.807798 | 4.238320e-50 | -8.615850e-47 |
| Msa1183140 | Msa1359400 | 0.865079 | 7.655872e-65 | -8.615850e-47 |
| Msa1196610 | Msa1359400 | -0.817596 | 3.038471e-52 | -8.615850e-47 |
| Msa1208450 | Msa1359400 | -0.819209 | 1.309654e-52 | -8.615850e-47 |
| Msa1209200 | Msa1359400 | 0.806605 | 7.584401e-50 | -8.615850e-47 |
| Msa0500470 | Msa1359400 | 0.832827 | 7.570742e-56 | -8.615850e-47 |
| Msa0518170 | Msa1359400 | 0.818577 | 1.823017e-52 | -8.615850e-47 |
| Msa0519160 | Msa1359400 | 0.832117 | 1.134789e-55 | -8.615850e-47 |
| Msa0523050 | Msa1359400 | 0.815309 | 9.873730e-52 | -8.615850e-47 |
| Msa0523430 | Msa1359400 | -0.801638 | 8.192115e-49 | -8.615850e-47 |
| Msa0523490 | Msa1359400 | -0.800473 | 1.417753e-48 | -8.615850e-47 |
| Msa0545050 | Msa1359400 | 0.827810 | 1.273668e-54 | -8.615850e-47 |
| Msa0576080 | Msa1359400 | -0.802578 | 5.248362e-49 | -8.615850e-47 |
| Msa0576120 | Msa1359400 | -0.838744 | 2.397160e-57 | -8.615850e-47 |
| Msa0581280 | Msa1359400 | 0.823081 | 1.677939e-53 | -8.615850e-47 |
| Msa0583360 | Msa1359400 | -0.801660 | 8.107216e-49 | -8.615850e-47 |
| Msa0600590 | Msa1359400 | 0.812239 | 4.684916e-51 | -8.615850e-47 |
| Msa0857940 | Msa1359400 | 0.814896 | 1.220015e-51 | -8.615850e-47 |
| Msa0862620 | Msa1359400 | 0.817584 | 3.057244e-52 | -8.615850e-47 |
| Msa0894710 | Msa1359400 | 0.815622 | 8.413581e-52 | -8.615850e-47 |
| Msa0954840 | Msa1359400 | 0.800984 | 1.115298e-48 | -8.615850e-47 |
| Msa0975320 | Msa1359400 | 0.806451 | 8.175398e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1359400 | MtrunA17_Chr1g0149811 | 96.958 | 263 | 7 | 1 | 1 | 263 | 1 | 262 | 0.0 | 532 |
| Msa1359400 | MtrunA17_Chr3g0137111 | 48.729 | 236 | 97 | 9 | 23 | 248 | 4 | 225 | 1.15e-57 | 183 |
| Msa1359400 | MtrunA17_Chr2g0333741 | 35.563 | 284 | 125 | 13 | 1 | 263 | 1 | 247 | 1.62e-31 | 117 |
| Msa1359400 | MtrunA17_Chr4g0001081 | 37.553 | 237 | 96 | 10 | 46 | 263 | 21 | 224 | 6.47e-31 | 114 |
| Msa1359400 | MtrunA17_Chr5g0434121 | 34.975 | 203 | 86 | 6 | 31 | 211 | 2 | 180 | 9.35e-26 | 101 |
| Msa1359400 | MtrunA17_Chr8g0376021 | 40.690 | 145 | 59 | 4 | 37 | 181 | 15 | 132 | 8.23e-25 | 98.6 |
| Msa1359400 | MtrunA17_Chr1g0161901 | 38.095 | 147 | 70 | 3 | 35 | 181 | 43 | 168 | 1.84e-24 | 98.2 |
| Msa1359400 | MtrunA17_Chr3g0110091 | 54.545 | 88 | 22 | 2 | 93 | 180 | 85 | 154 | 5.35e-24 | 96.7 |
| Msa1359400 | MtrunA17_Chr1g0207691 | 37.594 | 133 | 59 | 3 | 53 | 182 | 21 | 132 | 9.96e-19 | 81.6 |
| Msa1359400 | MtrunA17_Chr4g0072571 | 61.538 | 52 | 20 | 0 | 91 | 142 | 42 | 93 | 3.22e-16 | 74.3 |
| Msa1359400 | MtrunA17_Chr3g0081131 | 30.645 | 186 | 97 | 6 | 28 | 198 | 20 | 188 | 6.17e-16 | 75.9 |
| Msa1359400 | MtrunA17_Chr3g0081141 | 41.176 | 102 | 50 | 3 | 85 | 185 | 89 | 181 | 1.75e-14 | 72.0 |
| Msa1359400 | MtrunA17_Chr3g0081121 | 33.333 | 186 | 98 | 7 | 28 | 198 | 20 | 194 | 1.80e-14 | 71.6 |
| Msa1359400 | MtrunA17_Chr5g0396031 | 38.793 | 116 | 56 | 4 | 82 | 195 | 34 | 136 | 1.40e-13 | 69.7 |
| Msa1359400 | MtrunA17_Chr4g0053691 | 41.111 | 90 | 22 | 2 | 93 | 182 | 66 | 124 | 3.07e-13 | 66.2 |
| Msa1359400 | MtrunA17_Chr7g0251581 | 78.788 | 33 | 7 | 0 | 94 | 126 | 39 | 71 | 6.92e-13 | 65.5 |
| Msa1359400 | MtrunA17_Chr7g0254221 | 70.270 | 37 | 11 | 0 | 94 | 130 | 33 | 69 | 9.47e-13 | 64.3 |
| Msa1359400 | MtrunA17_Chr3g0079671 | 40.000 | 95 | 41 | 4 | 92 | 182 | 96 | 178 | 1.22e-11 | 63.5 |
| Msa1359400 | MtrunA17_Chr7g0224221 | 32.184 | 174 | 102 | 8 | 27 | 198 | 86 | 245 | 1.84e-11 | 63.5 |
| Msa1359400 | MtrunA17_Chr2g0310101 | 35.238 | 105 | 53 | 2 | 92 | 195 | 57 | 147 | 3.29e-11 | 61.2 |
| Msa1359400 | MtrunA17_Chr7g0222671 | 33.333 | 117 | 67 | 5 | 83 | 196 | 55 | 163 | 4.93e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1359400 | AT5G10970.1 | 42.616 | 237 | 90 | 9 | 49 | 263 | 60 | 272 | 4.17e-44 | 150 |
| Msa1359400 | AT5G25160.1 | 49.419 | 172 | 66 | 7 | 51 | 222 | 18 | 168 | 2.91e-39 | 137 |
| Msa1359400 | AT1G80730.1 | 37.395 | 238 | 127 | 7 | 35 | 263 | 4 | 228 | 1.95e-32 | 119 |
| Msa1359400 | AT1G66140.1 | 49.515 | 103 | 35 | 3 | 91 | 183 | 80 | 175 | 5.80e-22 | 92.0 |
| Msa1359400 | AT1G24625.1 | 42.727 | 110 | 37 | 4 | 74 | 179 | 33 | 120 | 1.25e-16 | 76.6 |
| Msa1359400 | AT5G14010.1 | 42.697 | 89 | 39 | 3 | 94 | 178 | 36 | 116 | 3.86e-14 | 68.9 |
| Msa1359400 | AT5G57520.1 | 77.778 | 36 | 8 | 0 | 94 | 129 | 50 | 85 | 5.08e-14 | 68.2 |
| Msa1359400 | AT1G67030.1 | 38.938 | 113 | 55 | 3 | 70 | 180 | 13 | 113 | 3.39e-13 | 67.0 |
| Msa1359400 | AT3G58070.1 | 31.638 | 177 | 92 | 7 | 23 | 190 | 23 | 179 | 6.47e-12 | 64.3 |
| Msa1359400 | AT5G01860.1 | 56.250 | 48 | 18 | 1 | 80 | 127 | 54 | 98 | 7.43e-11 | 60.8 |
Find 32 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAAACTCAAGAATCTTTA+TGG | 0.122503 | tig0016715:-28444 | Msa1359400:CDS |
| ATGGCTTCTTTGCCTCTTTA+TGG | 0.168485 | tig0016715:-28584 | Msa1359400:CDS |
| CTTTGTTTATTATGCATAAT+AGG | 0.201649 | tig0016715:+28617 | None:intergenic |
| CCTTCTCATGTTTCATCTAA+TGG | 0.202335 | tig0016715:-28506 | Msa1359400:CDS |
| CATGTTTCATCTAATGGATT+TGG | 0.228057 | tig0016715:-28500 | Msa1359400:CDS |
| AATGGATTTGGAAACTCTTA+TGG | 0.229174 | tig0016715:-28488 | Msa1359400:CDS |
| ATAGCTTCAGCTTCTGCTTT+TGG | 0.233602 | tig0016715:-28644 | Msa1359400:CDS |
| TTGCCTCTTTATGGTGCTTT+TGG | 0.261823 | tig0016715:-28575 | Msa1359400:CDS |
| ATGAAACATGAGAAGGCTTA+TGG | 0.351379 | tig0016715:+28513 | None:intergenic |
| CTCATAACTTCTTTAGATGT+TGG | 0.385754 | tig0016715:-28836 | Msa1359400:CDS |
| TGCAAAGGTGTGCTTGGACT+TGG | 0.428789 | tig0016715:+28998 | None:intergenic |
| TTCTATAGCTCACAAGCATT+AGG | 0.451109 | tig0016715:-28737 | Msa1359400:CDS |
| TTTGGTGGTAATAAACCTCT+TGG | 0.453972 | tig0016715:-28557 | Msa1359400:CDS |
| AAAGGTGTGCTTGGACTTGG+AGG | 0.516654 | tig0016715:+29001 | None:intergenic |
| TGGAATAGCAAAACTAGGTA+TGG | 0.517898 | tig0016715:-28411 | Msa1359400:CDS |
| ATCTTTATGGATCAACAACC+TGG | 0.518646 | tig0016715:-28431 | Msa1359400:CDS |
| CAACCTGGAATAGCAAAACT+AGG | 0.529841 | tig0016715:-28416 | Msa1359400:CDS |
| CCACCAAAAGCACCATAAAG+AGG | 0.536698 | tig0016715:+28572 | None:intergenic |
| CATCAAGAGGAAGTGTTGGT+AGG | 0.548091 | tig0016715:-28364 | Msa1359400:CDS |
| AAGTGTTGGTAGGTTTGAAG+TGG | 0.552808 | tig0016715:-28354 | Msa1359400:CDS |
| GAAGAAACAACAATCTATTG+AGG | 0.558110 | tig0016715:-28960 | Msa1359400:CDS |
| ATTAATGGAAAGACTAAGCA+AGG | 0.563627 | tig0016715:-29053 | None:intergenic |
| TATGGACATCATCATCATCA+TGG | 0.564611 | tig0016715:-28470 | Msa1359400:CDS |
| TCTTGAGAAGAGAAGATGCA+TGG | 0.579539 | tig0016715:+29028 | None:intergenic |
| GGTAGAAGAAGATATCACTA+AGG | 0.583712 | tig0016715:-28939 | Msa1359400:CDS |
| ACATCATCAAGAGGAAGTGT+TGG | 0.585725 | tig0016715:-28368 | Msa1359400:CDS |
| TATAGCTCACAAGCATTAGG+TGG | 0.606344 | tig0016715:-28734 | Msa1359400:CDS |
| AGAACAACAACATCATCAAG+AGG | 0.611847 | tig0016715:-28377 | Msa1359400:CDS |
| ATATTCATAATCATAGCTTG+AGG | 0.612882 | tig0016715:+28270 | None:intergenic |
| TAAACAAAGTTATGCAAACA+TGG | 0.616969 | tig0016715:-28603 | Msa1359400:CDS |
| CCATTAGATGAAACATGAGA+AGG | 0.626125 | tig0016715:+28506 | None:intergenic |
| GAGTGTGCTTGAATTCCAAG+AGG | 0.647089 | tig0016715:+28542 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTGTTTATTATGCATAAT+AGG | + | tig0016715:28715-28734 | None:intergenic | 20.0% |
| !!! | CTTTGTTTATTATGCATAAT+AGG | + | tig0016715:28715-28734 | None:intergenic | 20.0% |
| !!! | TTTTCATGTTGATTTTGCAA+AGG | + | tig0016715:28349-28368 | None:intergenic | 25.0% |
| ! | TAAACAAAGTTATGCAAACA+TGG | - | tig0016715:28726-28745 | Msa1359400:CDS | 25.0% |
| !!! | TTTTCATGTTGATTTTGCAA+AGG | + | tig0016715:28349-28368 | None:intergenic | 25.0% |
| ! | TAAACAAAGTTATGCAAACA+TGG | - | tig0016715:28726-28745 | Msa1359400:CDS | 25.0% |
| GAAGAAACAACAATCTATTG+AGG | - | tig0016715:28369-28388 | Msa1359400:CDS | 30.0% | |
| CTCATAACTTCTTTAGATGT+TGG | - | tig0016715:28493-28512 | Msa1359400:CDS | 30.0% | |
| !! | CATGTTTCATCTAATGGATT+TGG | - | tig0016715:28829-28848 | Msa1359400:CDS | 30.0% |
| ! | AATGGATTTGGAAACTCTTA+TGG | - | tig0016715:28841-28860 | Msa1359400:CDS | 30.0% |
| GTCAAACTCAAGAATCTTTA+TGG | - | tig0016715:28885-28904 | Msa1359400:CDS | 30.0% | |
| GAAGAAACAACAATCTATTG+AGG | - | tig0016715:28369-28388 | Msa1359400:CDS | 30.0% | |
| CTCATAACTTCTTTAGATGT+TGG | - | tig0016715:28493-28512 | Msa1359400:CDS | 30.0% | |
| !! | CATGTTTCATCTAATGGATT+TGG | - | tig0016715:28829-28848 | Msa1359400:CDS | 30.0% |
| ! | AATGGATTTGGAAACTCTTA+TGG | - | tig0016715:28841-28860 | Msa1359400:CDS | 30.0% |
| GTCAAACTCAAGAATCTTTA+TGG | - | tig0016715:28885-28904 | Msa1359400:CDS | 30.0% | |
| GGTAGAAGAAGATATCACTA+AGG | - | tig0016715:28390-28409 | Msa1359400:CDS | 35.0% | |
| TTCTATAGCTCACAAGCATT+AGG | - | tig0016715:28592-28611 | Msa1359400:CDS | 35.0% | |
| ! | TCTCTCTTTTGTGAGCATTT+TGG | + | tig0016715:28624-28643 | None:intergenic | 35.0% |
| GAGAGATCAATAGCAAAAAG+AGG | - | tig0016715:28640-28659 | Msa1359400:CDS | 35.0% | |
| GCAAAAAGAGGACAAAGATT+TGG | - | tig0016715:28652-28671 | Msa1359400:CDS | 35.0% | |
| ! | TTTGGTGGTAATAAACCTCT+TGG | - | tig0016715:28772-28791 | Msa1359400:CDS | 35.0% |
| ATGAAACATGAGAAGGCTTA+TGG | + | tig0016715:28819-28838 | None:intergenic | 35.0% | |
| CCATTAGATGAAACATGAGA+AGG | + | tig0016715:28826-28845 | None:intergenic | 35.0% | |
| CCTTCTCATGTTTCATCTAA+TGG | - | tig0016715:28823-28842 | Msa1359400:CDS | 35.0% | |
| TATGGACATCATCATCATCA+TGG | - | tig0016715:28859-28878 | Msa1359400:CDS | 35.0% | |
| ATCTTTATGGATCAACAACC+TGG | - | tig0016715:28898-28917 | Msa1359400:CDS | 35.0% | |
| !!! | ATACCTAGTTTTGCTATTCC+AGG | + | tig0016715:28919-28938 | None:intergenic | 35.0% |
| TGGAATAGCAAAACTAGGTA+TGG | - | tig0016715:28918-28937 | Msa1359400:CDS | 35.0% | |
| AGAACAACAACATCATCAAG+AGG | - | tig0016715:28952-28971 | Msa1359400:CDS | 35.0% | |
| GGTAGAAGAAGATATCACTA+AGG | - | tig0016715:28390-28409 | Msa1359400:CDS | 35.0% | |
| TTCTATAGCTCACAAGCATT+AGG | - | tig0016715:28592-28611 | Msa1359400:CDS | 35.0% | |
| ! | TCTCTCTTTTGTGAGCATTT+TGG | + | tig0016715:28624-28643 | None:intergenic | 35.0% |
| GAGAGATCAATAGCAAAAAG+AGG | - | tig0016715:28640-28659 | Msa1359400:CDS | 35.0% | |
| GCAAAAAGAGGACAAAGATT+TGG | - | tig0016715:28652-28671 | Msa1359400:CDS | 35.0% | |
| ! | TTTGGTGGTAATAAACCTCT+TGG | - | tig0016715:28772-28791 | Msa1359400:CDS | 35.0% |
| ATGAAACATGAGAAGGCTTA+TGG | + | tig0016715:28819-28838 | None:intergenic | 35.0% | |
| CCATTAGATGAAACATGAGA+AGG | + | tig0016715:28826-28845 | None:intergenic | 35.0% | |
| CCTTCTCATGTTTCATCTAA+TGG | - | tig0016715:28823-28842 | Msa1359400:CDS | 35.0% | |
| TATGGACATCATCATCATCA+TGG | - | tig0016715:28859-28878 | Msa1359400:CDS | 35.0% | |
| ATCTTTATGGATCAACAACC+TGG | - | tig0016715:28898-28917 | Msa1359400:CDS | 35.0% | |
| !!! | ATACCTAGTTTTGCTATTCC+AGG | + | tig0016715:28919-28938 | None:intergenic | 35.0% |
| TGGAATAGCAAAACTAGGTA+TGG | - | tig0016715:28918-28937 | Msa1359400:CDS | 35.0% | |
| AGAACAACAACATCATCAAG+AGG | - | tig0016715:28952-28971 | Msa1359400:CDS | 35.0% | |
| TCTTGAGAAGAGAAGATGCA+TGG | + | tig0016715:28304-28323 | None:intergenic | 40.0% | |
| !! | TGATTTTGCAAAGGTGTGCT+TGG | + | tig0016715:28340-28359 | None:intergenic | 40.0% |
| TATAGCTCACAAGCATTAGG+TGG | - | tig0016715:28595-28614 | Msa1359400:CDS | 40.0% | |
| ! | ATAGCTTCAGCTTCTGCTTT+TGG | - | tig0016715:28685-28704 | Msa1359400:CDS | 40.0% |
| ATGGCTTCTTTGCCTCTTTA+TGG | - | tig0016715:28745-28764 | Msa1359400:CDS | 40.0% | |
| ! | TTGCCTCTTTATGGTGCTTT+TGG | - | tig0016715:28754-28773 | Msa1359400:CDS | 40.0% |
| GACATCATCATCATCATGGT+TGG | - | tig0016715:28863-28882 | Msa1359400:CDS | 40.0% | |
| CAACCTGGAATAGCAAAACT+AGG | - | tig0016715:28913-28932 | Msa1359400:CDS | 40.0% | |
| ! | ACATCATCAAGAGGAAGTGT+TGG | - | tig0016715:28961-28980 | Msa1359400:CDS | 40.0% |
| !! | AAGTGTTGGTAGGTTTGAAG+TGG | - | tig0016715:28975-28994 | Msa1359400:CDS | 40.0% |
| TCTTGAGAAGAGAAGATGCA+TGG | + | tig0016715:28304-28323 | None:intergenic | 40.0% | |
| !! | TGATTTTGCAAAGGTGTGCT+TGG | + | tig0016715:28340-28359 | None:intergenic | 40.0% |
| TATAGCTCACAAGCATTAGG+TGG | - | tig0016715:28595-28614 | Msa1359400:CDS | 40.0% | |
| ! | ATAGCTTCAGCTTCTGCTTT+TGG | - | tig0016715:28685-28704 | Msa1359400:CDS | 40.0% |
| ATGGCTTCTTTGCCTCTTTA+TGG | - | tig0016715:28745-28764 | Msa1359400:CDS | 40.0% | |
| ! | TTGCCTCTTTATGGTGCTTT+TGG | - | tig0016715:28754-28773 | Msa1359400:CDS | 40.0% |
| GACATCATCATCATCATGGT+TGG | - | tig0016715:28863-28882 | Msa1359400:CDS | 40.0% | |
| CAACCTGGAATAGCAAAACT+AGG | - | tig0016715:28913-28932 | Msa1359400:CDS | 40.0% | |
| ! | ACATCATCAAGAGGAAGTGT+TGG | - | tig0016715:28961-28980 | Msa1359400:CDS | 40.0% |
| !! | AAGTGTTGGTAGGTTTGAAG+TGG | - | tig0016715:28975-28994 | Msa1359400:CDS | 40.0% |
| !! | CCACCAAAAGCACCATAAAG+AGG | + | tig0016715:28760-28779 | None:intergenic | 45.0% |
| ! | CCTCTTTATGGTGCTTTTGG+TGG | - | tig0016715:28757-28776 | Msa1359400:CDS | 45.0% |
| GAGTGTGCTTGAATTCCAAG+AGG | + | tig0016715:28790-28809 | None:intergenic | 45.0% | |
| !! | CATCAAGAGGAAGTGTTGGT+AGG | - | tig0016715:28965-28984 | Msa1359400:CDS | 45.0% |
| !! | CCACCAAAAGCACCATAAAG+AGG | + | tig0016715:28760-28779 | None:intergenic | 45.0% |
| ! | CCTCTTTATGGTGCTTTTGG+TGG | - | tig0016715:28757-28776 | Msa1359400:CDS | 45.0% |
| GAGTGTGCTTGAATTCCAAG+AGG | + | tig0016715:28790-28809 | None:intergenic | 45.0% | |
| !! | CATCAAGAGGAAGTGTTGGT+AGG | - | tig0016715:28965-28984 | Msa1359400:CDS | 45.0% |
| ! | AAAGGTGTGCTTGGACTTGG+AGG | + | tig0016715:28331-28350 | None:intergenic | 50.0% |
| ! | TGCAAAGGTGTGCTTGGACT+TGG | + | tig0016715:28334-28353 | None:intergenic | 50.0% |
| ! | AAAGGTGTGCTTGGACTTGG+AGG | + | tig0016715:28331-28350 | None:intergenic | 50.0% |
| ! | TGCAAAGGTGTGCTTGGACT+TGG | + | tig0016715:28334-28353 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0016715 | gene | 28280 | 29071 | 28280 | ID=Msa1359400;Name=Msa1359400 |
| tig0016715 | mRNA | 28280 | 29071 | 28280 | ID=Msa1359400-mRNA-1;Parent=Msa1359400;Name=Msa1359400-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|263 |
| tig0016715 | exon | 28280 | 29071 | 28280 | ID=Msa1359400-mRNA-1:exon:11350;Parent=Msa1359400-mRNA-1 |
| tig0016715 | CDS | 28280 | 29071 | 28280 | ID=Msa1359400-mRNA-1:cds;Parent=Msa1359400-mRNA-1 |
| Gene Sequence |
| Protein sequence |
