AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0189880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0189880 MtrunA17_Chr2g0279241 93.404 379 20 2 1 379 1 374 0.0 674
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0189880 AT5G64230.1 42.268 388 175 7 18 375 10 378 1.09e-89 275
Msa0189880 AT3G19920.1 32.911 316 163 5 92 371 111 413 2.68e-43 155
Msa0189880 AT3G19920.2 32.911 316 163 5 92 371 152 454 4.35e-43 156

Find 137 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTATACCATTCATAAATT+TGG 0.159458 2_1:-5339363 Msa0189880:three_prime_UTR
TGGGCGGTGGCGGATAATTT+AGG 0.162639 2_1:-5341232 Msa0189880:CDS
TTCAAAGAAGCCAAAGATTT+TGG 0.169588 2_1:-5339889 Msa0189880:CDS
TTTCGCGTTCTTCCCCATTT+TGG 0.215075 2_1:+5341945 None:intergenic
GAGGAGGGTGTGGAGGGTGG+TGG 0.218739 2_1:-5341383 Msa0189880:CDS
CGAAAAGAAAATAACCAAAA+TGG 0.222336 2_1:-5341959 Msa0189880:exon
TACTGCTGATCCTCGTTTGC+AGG 0.243765 2_1:-5341197 Msa0189880:CDS
AGCTTTAAATGTGTGCAAAA+TGG 0.264969 2_1:+5342024 None:intergenic
TTCGTCTGCGCCAAAATCTT+TGG 0.282865 2_1:+5339879 None:intergenic
GGGTGTGGAGGGTGGTGGGG+TGG 0.285106 2_1:-5341378 Msa0189880:CDS
GGAGGGTGTGGAGGGTGGTG+GGG 0.287513 2_1:-5341381 Msa0189880:CDS
CTCTCATTGATGCTCAATGC+TGG 0.290296 2_1:+5339757 None:intergenic
GAGGAGGAGGGTGTGGAGGG+TGG 0.291263 2_1:-5341386 Msa0189880:CDS
TGGGGCATCGATGCCATTAC+TGG 0.294293 2_1:+5339777 None:intergenic
AAGAACAGGTTCTTTCTCTT+TGG 0.297051 2_1:-5339678 Msa0189880:CDS
GCTTCGCTTGTTAAACTTGC+AGG 0.297214 2_1:-5341175 Msa0189880:intron
TGGTTTAAGTGGTGGTGTTC+CGG 0.299500 2_1:-5341335 Msa0189880:CDS
GTGGAGGGTGGTGGGGTGGA+AGG 0.327039 2_1:-5341374 Msa0189880:CDS
AGGAGGGTGTGGAGGGTGGT+GGG 0.327315 2_1:-5341382 Msa0189880:CDS
TGGTGAGGAGGAGGGTGTGG+AGG 0.336421 2_1:-5341390 Msa0189880:CDS
GGGCGGTGGCGGATAATTTA+GGG 0.342904 2_1:-5341231 Msa0189880:CDS
GTGATGGAGGAGAGTGGTAT+AGG 0.357153 2_1:-5341414 Msa0189880:CDS
AATTGGCAGCGGAGTTGGTT+TGG 0.367956 2_1:-5341306 Msa0189880:CDS
GAAAAGAAAATAACCAAAAT+GGG 0.374894 2_1:-5341958 Msa0189880:exon
GTGGAAGGTGAAGGGAGGTG+AGG 0.381406 2_1:-5341359 Msa0189880:CDS
GGATGATGAAACGGAGGGTT+TGG 0.381540 2_1:+5341788 None:intergenic
GGATGGTGAGGAGGAGGGTG+TGG 0.384248 2_1:-5341393 Msa0189880:CDS
GCATAACATGATTGGAGGTT+TGG 0.384676 2_1:+5339619 None:intergenic
GGTGAAAAGGGAAGAGATTA+GGG 0.395429 2_1:+5341870 None:intergenic
GAATGAAACGGAGGAGGAAG+AGG 0.397994 2_1:+5341755 None:intergenic
GTTGCTTTGTCTTCAGCGTT+TGG 0.402044 2_1:-5341499 Msa0189880:CDS
TGGTGTTCCGGCGGAGAAAT+TGG 0.402368 2_1:-5341323 Msa0189880:CDS
GGTGAGGAGGAGGGTGTGGA+GGG 0.415395 2_1:-5341389 Msa0189880:CDS
TGGTGAAAAGGGAAGAGATT+AGG 0.417039 2_1:+5341869 None:intergenic
GGTATAGGATGGTGAGGAGG+AGG 0.417760 2_1:-5341399 Msa0189880:CDS
CACACATTTAAAGCTAGAAA+AGG 0.421159 2_1:-5342016 Msa0189880:five_prime_UTR
CATGGCTGCCATTGTTGTGC+AGG 0.422978 2_1:-5339801 Msa0189880:CDS
GGTGGTGGGGTGGAAGGTGA+AGG 0.423207 2_1:-5341368 Msa0189880:CDS
TTGCTTTGTCTTCAGCGTTT+GGG 0.424552 2_1:-5341498 Msa0189880:CDS
TATGAATGGTATAAGTATAA+CGG 0.426115 2_1:+5339370 None:intergenic
CTTGCTGTTTAAGTTTAATA+CGG 0.427939 2_1:-5339309 Msa0189880:three_prime_UTR
CGAAGCAGTTCGATCGTTAA+CGG 0.429812 2_1:-5341581 Msa0189880:CDS
TCTCATTGATGCTCAATGCT+GGG 0.437571 2_1:+5339758 None:intergenic
GTGGGGTGGAAGGTGAAGGG+AGG 0.443740 2_1:-5341364 Msa0189880:CDS
CAACGGCGGTGGTGGTGAAA+AGG 0.444067 2_1:+5341857 None:intergenic
GAATGTCAAAGAACTATCAT+AGG 0.446139 2_1:-5339433 Msa0189880:three_prime_UTR
TGGAAGGTGAAGGGAGGTGA+GGG 0.448134 2_1:-5341358 Msa0189880:CDS
CATCAATGAGAGGGCTGAGT+TGG 0.451437 2_1:-5339746 Msa0189880:CDS
AAGTTCTAAGTTATATCAAC+AGG 0.453490 2_1:-5339473 Msa0189880:three_prime_UTR
GGAGAAATTGGCAGCGGAGT+TGG 0.458127 2_1:-5341311 Msa0189880:CDS
TGTAGTTGACAGAGAAGGAG+AGG 0.458499 2_1:+5341818 None:intergenic
TACACTTCCAAATTTATGAA+TGG 0.468718 2_1:+5339356 None:intergenic
GGCTGAGTTGGAGAGAGTGT+TGG 0.468736 2_1:-5339734 Msa0189880:CDS
GAGGGTGAACAAGGTGATGG+AGG 0.472780 2_1:-5341427 Msa0189880:CDS
AGGATGAGCAATGTGTAAAA+TGG 0.472912 2_1:+5339650 None:intergenic
GTTCGGAGGAGCGGCGGAAG+TGG 0.474833 2_1:+5341892 None:intergenic
GGAAAGCATTATAGAGGGGT+TGG 0.475155 2_1:-5339713 Msa0189880:CDS
GTTGACAGAGAAGGAGAGGA+AGG 0.478361 2_1:+5341822 None:intergenic
AGGAGCGGCGGAAGTGGTAG+TGG 0.486154 2_1:+5341898 None:intergenic
TCCAATCATGTTATGCTCGC+TGG 0.490686 2_1:-5339612 Msa0189880:CDS
GTATAGGATGGTGAGGAGGA+GGG 0.494671 2_1:-5341398 Msa0189880:CDS
CTGATGAAGCTCTTCAGAGA+TGG 0.495288 2_1:-5341252 Msa0189880:CDS
AAGGTGGCGGAGAGAGATCA+AGG 0.501719 2_1:-5341451 Msa0189880:CDS
GGAGGTGAGGGTGGTTTAAG+TGG 0.502354 2_1:-5341346 Msa0189880:CDS
GGAGGAAGAGGTAGAAAGTG+AGG 0.503057 2_1:+5341767 None:intergenic
AGAAAGTGAGGATGATGAAA+CGG 0.504841 2_1:+5341779 None:intergenic
CTTTGTCTTCAGCGTTTGGG+AGG 0.507256 2_1:-5341495 Msa0189880:CDS
AAGAAGGAATGAAACGGAGG+AGG 0.508172 2_1:+5341749 None:intergenic
AGAGAAGGAGAGGAAGGTGG+TGG 0.511463 2_1:+5341828 None:intergenic
GTGTTGGAAAGCATTATAGA+GGG 0.513012 2_1:-5339718 Msa0189880:CDS
AGTGTTGGAAAGCATTATAG+AGG 0.513358 2_1:-5339719 Msa0189880:CDS
AACGGCGGTGGTGGTGAAAA+GGG 0.514502 2_1:+5341858 None:intergenic
GTGGTGGGGTGGAAGGTGAA+GGG 0.517185 2_1:-5341367 Msa0189880:CDS
TCTTCAGAGATGGGCGGTGG+CGG 0.518241 2_1:-5341242 Msa0189880:CDS
ACCAGCGAGCATAACATGAT+TGG 0.520683 2_1:+5339611 None:intergenic
GAGAGTGGTATAGGATGGTG+AGG 0.520943 2_1:-5341405 Msa0189880:CDS
ACAACTACTAGAGCTGAAGT+TGG 0.523016 2_1:+5339499 None:intergenic
ACATTCAAGAAATATTTAAG+TGG 0.523311 2_1:+5339450 None:intergenic
GAGTTTGTAGTTGACAGAGA+AGG 0.525446 2_1:+5341813 None:intergenic
AGCGAGCATAACATGATTGG+AGG 0.527880 2_1:+5339614 None:intergenic
GGAACAAGAGAAGCAAGAAC+AGG 0.529481 2_1:-5339692 Msa0189880:CDS
TGGATCGACAAAGGTTGACT+TGG 0.529788 2_1:+5342044 None:intergenic
GAGATTAGGGTTCGGAGGAG+CGG 0.531244 2_1:+5341883 None:intergenic
GGCAGCGGAGTTGGTTTGGA+TGG 0.531399 2_1:-5341302 Msa0189880:CDS
CTCATTGATGCTCAATGCTG+GGG 0.535606 2_1:+5339759 None:intergenic
TGGGAGGGCGTTGAGGCAGT+TGG 0.535862 2_1:-5341479 Msa0189880:CDS
AGTGGTATAGGATGGTGAGG+AGG 0.536537 2_1:-5341402 Msa0189880:CDS
GACAGAGAAGGAGAGGAAGG+TGG 0.536632 2_1:+5341825 None:intergenic
TCCGCTGCCAATTTCTCCGC+CGG 0.537453 2_1:+5341316 None:intergenic
CACATTGCTCATCCTCTGAC+TGG 0.539240 2_1:-5339642 Msa0189880:CDS
AAGGTGAAGGGAGGTGAGGG+TGG 0.541781 2_1:-5341355 Msa0189880:CDS
GATCAAGGAGAGGGTGAACA+AGG 0.543520 2_1:-5341436 Msa0189880:CDS
CAGCGTTTGGGAGGGCGTTG+AGG 0.545073 2_1:-5341486 Msa0189880:CDS
AAAGGGAAGAGATTAGGGTT+CGG 0.546803 2_1:+5341875 None:intergenic
TAAGTTTAATACGGTTGTTG+TGG 0.551016 2_1:-5339300 None:intergenic
CAAGATGAAGCAATGCATGC+AGG 0.557965 2_1:-5339845 Msa0189880:CDS
TGATGAAGCTCTTCAGAGAT+GGG 0.561498 2_1:-5341251 Msa0189880:CDS
ATTAGGGTTCGGAGGAGCGG+CGG 0.562847 2_1:+5341886 None:intergenic
TTTGTCTTCAGCGTTTGGGA+GGG 0.563106 2_1:-5341494 Msa0189880:CDS
TTGTTGTGCAGGGCCAGTAA+TGG 0.566356 2_1:-5339790 Msa0189880:CDS
TGTTGGAAAGCATTATAGAG+GGG 0.568319 2_1:-5339717 Msa0189880:CDS
CGGCGGAAGTGGTAGTGGCA+TGG 0.574599 2_1:+5341903 None:intergenic
AACAGCAAGAACCAACATAG+TGG 0.577713 2_1:+5339323 None:intergenic
GGAGAGGGTGAACAAGGTGA+TGG 0.578772 2_1:-5341430 Msa0189880:CDS
AGCTCTTCAGAGATGGGCGG+TGG 0.579015 2_1:-5341245 Msa0189880:CDS
GTAACGAATGAACACCTTCG+AGG 0.580463 2_1:+5341536 None:intergenic
GGGAAGAGATTAGGGTTCGG+AGG 0.585432 2_1:+5341878 None:intergenic
AACAAGGTGATGGAGGAGAG+TGG 0.587077 2_1:-5341420 Msa0189880:CDS
AGCATATCAAGCCACTATGT+TGG 0.590782 2_1:-5339334 Msa0189880:three_prime_UTR
GGTGAGGGTGGTTTAAGTGG+TGG 0.593095 2_1:-5341343 Msa0189880:CDS
AAGTGAGGATGATGAAACGG+AGG 0.593185 2_1:+5341782 None:intergenic
GTTGAGGCAGTTGGAAGTGA+AGG 0.595100 2_1:-5341470 Msa0189880:CDS
GCGGAGAGAGATCAAGGAGA+GGG 0.598505 2_1:-5341445 Msa0189880:CDS
ACAAGCGAAGCCTGCAAACG+AGG 0.601298 2_1:+5341187 None:intergenic
TTGGAGGTTTGGCCAGTCAG+AGG 0.607532 2_1:+5339630 None:intergenic
GGTTTGGATGGTGAAGAAGA+TGG 0.612162 2_1:-5341290 Msa0189880:CDS
AGCATTGAGCATCAATGAGA+GGG 0.612874 2_1:-5339755 Msa0189880:CDS
CTGAGAAAGAGCACGTCCGA+TGG 0.614254 2_1:+5341648 None:intergenic
GAGGCAGTTGGAAGTGAAGG+TGG 0.615671 2_1:-5341467 Msa0189880:CDS
TCCGGCGGAGAAATTGGCAG+CGG 0.615724 2_1:-5341317 Msa0189880:CDS
GTGAAGAAGATGGTGGAGTG+TGG 0.615961 2_1:-5341280 Msa0189880:CDS
ATGGCTGCCATTGTTGTGCA+GGG 0.617221 2_1:-5339800 Msa0189880:CDS
CAGCATTGAGCATCAATGAG+AGG 0.617619 2_1:-5339756 Msa0189880:CDS
AGTGAGGATGATGAAACGGA+GGG 0.622730 2_1:+5341783 None:intergenic
GGCGGAGAGAGATCAAGGAG+AGG 0.623247 2_1:-5341446 Msa0189880:CDS
GCAGTTGGAAGTGAAGGTGG+CGG 0.625405 2_1:-5341464 Msa0189880:CDS
ACACGAAACGACACTGCCAT+CGG 0.626709 2_1:-5341664 Msa0189880:CDS
TTACTGGCCCTGCACAACAA+TGG 0.626932 2_1:+5339793 None:intergenic
TTTAAGTGGTGGTGTTCCGG+CGG 0.628276 2_1:-5341332 Msa0189880:CDS
ATGAGCAATGTGTAAAATGG+TGG 0.634351 2_1:+5339653 None:intergenic
TTGGATGGTGAAGAAGATGG+TGG 0.645396 2_1:-5341287 Msa0189880:CDS
TGGAGGAGAGTGGTATAGGA+TGG 0.647947 2_1:-5341410 Msa0189880:CDS
ATCGACGATAATCACCTCGA+AGG 0.649591 2_1:-5341550 Msa0189880:CDS
AGTTAAAGATGTTACAGACA+TGG 0.655695 2_1:-5339819 Msa0189880:CDS
AAAAGAAAATAACCAAAATG+GGG 0.656227 2_1:-5341957 Msa0189880:exon
TGAAGCTCTTCAGAGATGGG+CGG 0.671325 2_1:-5341248 Msa0189880:CDS
TGTGCAAAATGGATCGACAA+AGG 0.696464 2_1:+5342035 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTTTTAAATCATTTAAAA+TGG - chr2_1:5340829-5340848 Msa0189880:intron 10.0%
!! ATATGAAGAATGAATATATA+TGG + chr2_1:5340201-5340220 None:intergenic 15.0%
!!! TAATTTTACAATTTTGAACA+TGG - chr2_1:5340257-5340276 Msa0189880:intron 15.0%
!!! TTTAAATCATTTAAAATGGT+TGG - chr2_1:5340833-5340852 Msa0189880:intron 15.0%
!!! AAATGTATTTCTAGTTAAAA+TGG - chr2_1:5341215-5341234 Msa0189880:CDS 15.0%
!!! AATGTATTTCTAGTTAAAAT+GGG - chr2_1:5341216-5341235 Msa0189880:CDS 15.0%
!! GAAAAGAAAATAACCAAAAT+GGG - chr2_1:5339390-5339409 Msa0189880:three_prime_UTR 20.0%
!! AAAAGAAAATAACCAAAATG+GGG - chr2_1:5339391-5339410 Msa0189880:three_prime_UTR 20.0%
!!! TTTTCATTGAAGAAAAAAGA+AGG + chr2_1:5339618-5339637 None:intergenic 20.0%
!! TCTTATTGTATGTATAACTT+TGG + chr2_1:5340787-5340806 None:intergenic 20.0%
!! ATTTATGATCAAGAATCTAA+TGG + chr2_1:5341118-5341137 None:intergenic 20.0%
!! TCAAAGCATAAACTAAAAAT+TGG + chr2_1:5341196-5341215 None:intergenic 20.0%
!! TACAGAGTAGTTAAATTTAT+AGG - chr2_1:5341369-5341388 Msa0189880:CDS 20.0%
!! ACATTCAAGAAATATTTAAG+TGG + chr2_1:5341901-5341920 None:intergenic 20.0%
!! TATGAATGGTATAAGTATAA+CGG + chr2_1:5341981-5342000 None:intergenic 20.0%
!! ACTTATACCATTCATAAATT+TGG - chr2_1:5341985-5342004 Msa0189880:five_prime_UTR 20.0%
! CGAAAAGAAAATAACCAAAA+TGG - chr2_1:5339389-5339408 Msa0189880:three_prime_UTR 25.0%
! AGAAAAAAGAAGGAATGAAA+CGG + chr2_1:5339608-5339627 None:intergenic 25.0%
! TAACTTTGGATATAATTTCC+CGG + chr2_1:5340773-5340792 None:intergenic 25.0%
! TTGATCATAAATAACTTCCA+AGG - chr2_1:5341126-5341145 Msa0189880:intron 25.0%
! TAAACTAAAAATTGGATGAG+AGG + chr2_1:5341188-5341207 None:intergenic 25.0%
!!! GTTCAAATTTAGAAGTGTTT+AGG - chr2_1:5341273-5341292 Msa0189880:CDS 25.0%
!!! AAGTTCTAAGTTATATCAAC+AGG - chr2_1:5341875-5341894 Msa0189880:CDS 25.0%
! TACACTTCCAAATTTATGAA+TGG + chr2_1:5341995-5342014 None:intergenic 25.0%
!!! CTTGCTGTTTAAGTTTAATA+CGG - chr2_1:5342039-5342058 Msa0189880:five_prime_UTR 25.0%
!! AGCTTTAAATGTGTGCAAAA+TGG + chr2_1:5339327-5339346 None:intergenic 30.0%
! CACACATTTAAAGCTAGAAA+AGG - chr2_1:5339332-5339351 Msa0189880:three_prime_UTR 30.0%
! TCGTATTTTCTTGAACTAAC+TGG + chr2_1:5339750-5339769 None:intergenic 30.0%
CCAAGTAACTTTGATAAAGA+TGG + chr2_1:5340344-5340363 None:intergenic 30.0%
CCATCTTTATCAAAGTTACT+TGG - chr2_1:5340341-5340360 Msa0189880:intron 30.0%
TTTCAAACCACCTATATTCT+CGG + chr2_1:5340367-5340386 None:intergenic 30.0%
AAAATATTAGCTCGAGAATG+TGG - chr2_1:5340384-5340403 Msa0189880:intron 30.0%
!! TTGGAAAATTTCTAAGGCAA+AGG - chr2_1:5340418-5340437 Msa0189880:intron 30.0%
!! TTTTGTCTTGTTGCATTACA+AGG + chr2_1:5340514-5340533 None:intergenic 30.0%
TTTAAGCCTTTGTACTCATT+AGG - chr2_1:5340726-5340745 Msa0189880:intron 30.0%
AACTTTGGATATAATTTCCC+GGG + chr2_1:5340772-5340791 None:intergenic 30.0%
TCTTGTCTCCAAGTTTATAA+TGG - chr2_1:5340975-5340994 Msa0189880:intron 30.0%
TTCATTGGCCATTATAAACT+TGG + chr2_1:5340986-5341005 None:intergenic 30.0%
AATGGCCAATGAACTTATTA+TGG - chr2_1:5340993-5341012 Msa0189880:intron 30.0%
ATGGCCAATGAACTTATTAT+GGG - chr2_1:5340994-5341013 Msa0189880:intron 30.0%
TAAATAACTTCCAAGGATTG+TGG - chr2_1:5341133-5341152 Msa0189880:intron 30.0%
!! AACCACTATGAAGTATTGTT+TGG + chr2_1:5341256-5341275 None:intergenic 30.0%
GCGAACATTAAAGATATCAT+AGG + chr2_1:5341403-5341422 None:intergenic 30.0%
! TTCAAAGAAGCCAAAGATTT+TGG - chr2_1:5341459-5341478 Msa0189880:CDS 30.0%
AGTTAAAGATGTTACAGACA+TGG - chr2_1:5341529-5341548 Msa0189880:CDS 30.0%
GAATGTCAAAGAACTATCAT+AGG - chr2_1:5341915-5341934 Msa0189880:CDS 30.0%
!! GGTTATTTTCTTTTCGTACG+CGG + chr2_1:5339385-5339404 None:intergenic 35.0%
AGAAAGTGAGGATGATGAAA+CGG + chr2_1:5339572-5339591 None:intergenic 35.0%
AAAAAGAAGGAATGAAACGG+AGG + chr2_1:5339605-5339624 None:intergenic 35.0%
! GAACTTATTCTGCGTTTTGA+TGG + chr2_1:5339655-5339674 None:intergenic 35.0%
! GGTAGGTTGGAAAATTTCTA+AGG - chr2_1:5340412-5340431 Msa0189880:intron 35.0%
!! GAAAATTTCTAAGGCAAAGG+AGG - chr2_1:5340421-5340440 Msa0189880:intron 35.0%
TCCAACTTGTAATTCTAGCA+AGG - chr2_1:5340543-5340562 Msa0189880:intron 35.0%
CCAACTTGTAATTCTAGCAA+GGG - chr2_1:5340544-5340563 Msa0189880:intron 35.0%
! ATTGTAGCTTTTGACACCAA+AGG - chr2_1:5340674-5340693 Msa0189880:intron 35.0%
TTAAGACAAATCGTTGCCTT+TGG + chr2_1:5340693-5340712 None:intergenic 35.0%
CTAATGCCTAATGAGTACAA+AGG + chr2_1:5340735-5340754 None:intergenic 35.0%
! TTAGATTAGACTAGTTGTCC+CGG - chr2_1:5340751-5340770 Msa0189880:intron 35.0%
TGGCCAATGAACTTATTATG+GGG - chr2_1:5340995-5341014 Msa0189880:intron 35.0%
AAGCCCCATAATAAGTTCAT+TGG + chr2_1:5341001-5341020 None:intergenic 35.0%
CTCCAAACAATACTTCATAG+TGG - chr2_1:5341251-5341270 Msa0189880:CDS 35.0%
!! AGTGTTGGAAAGCATTATAG+AGG - chr2_1:5341629-5341648 Msa0189880:CDS 35.0%
!! GTGTTGGAAAGCATTATAGA+GGG - chr2_1:5341630-5341649 Msa0189880:CDS 35.0%
!! TGTTGGAAAGCATTATAGAG+GGG - chr2_1:5341631-5341650 Msa0189880:CDS 35.0%
AAGAACAGGTTCTTTCTCTT+TGG - chr2_1:5341670-5341689 Msa0189880:CDS 35.0%
ATGAGCAATGTGTAAAATGG+TGG + chr2_1:5341698-5341717 None:intergenic 35.0%
AGGATGAGCAATGTGTAAAA+TGG + chr2_1:5341701-5341720 None:intergenic 35.0%
TGTGCAAAATGGATCGACAA+AGG + chr2_1:5339316-5339335 None:intergenic 40.0%
AAAGGGAAGAGATTAGGGTT+CGG + chr2_1:5339476-5339495 None:intergenic 40.0%
GGTGAAAAGGGAAGAGATTA+GGG + chr2_1:5339481-5339500 None:intergenic 40.0%
TGGTGAAAAGGGAAGAGATT+AGG + chr2_1:5339482-5339501 None:intergenic 40.0%
GAGTTTGTAGTTGACAGAGA+AGG + chr2_1:5339538-5339557 None:intergenic 40.0%
!! CTTATTCTGCGTTTTGATGG+TGG + chr2_1:5339652-5339671 None:intergenic 40.0%
TTGCTTTGTCTTCAGCGTTT+GGG - chr2_1:5339850-5339869 Msa0189880:CDS 40.0%
TGATGAAGCTCTTCAGAGAT+GGG - chr2_1:5340097-5340116 Msa0189880:intron 40.0%
AGTTACTTGGCCGAGAATAT+AGG - chr2_1:5340354-5340373 Msa0189880:intron 40.0%
GCAAAGGAGGAAAGATGATA+AGG - chr2_1:5340434-5340453 Msa0189880:intron 40.0%
CACTTTGTCAAAGCTGTTTG+TGG - chr2_1:5340473-5340492 Msa0189880:intron 40.0%
CCCTTGCTAGAATTACAAGT+TGG + chr2_1:5340547-5340566 None:intergenic 40.0%
! TAGATTAGACTAGTTGTCCC+GGG - chr2_1:5340752-5340771 Msa0189880:intron 40.0%
TTTCAGTCACAGCAACATTC+TGG - chr2_1:5340947-5340966 Msa0189880:intron 40.0%
GAGAGGTAACTCTTCATTCT+AGG + chr2_1:5341171-5341190 None:intergenic 40.0%
CTTTGAACAGAAATGCTGCT+TGG + chr2_1:5341446-5341465 None:intergenic 40.0%
TCTCATTGATGCTCAATGCT+GGG + chr2_1:5341593-5341612 None:intergenic 40.0%
! AGCATTGAGCATCAATGAGA+GGG - chr2_1:5341593-5341612 Msa0189880:CDS 40.0%
GCATAACATGATTGGAGGTT+TGG + chr2_1:5341732-5341751 None:intergenic 40.0%
ACAACTACTAGAGCTGAAGT+TGG + chr2_1:5341852-5341871 None:intergenic 40.0%
AGCATATCAAGCCACTATGT+TGG - chr2_1:5342014-5342033 Msa0189880:five_prime_UTR 40.0%
AACAGCAAGAACCAACATAG+TGG + chr2_1:5342028-5342047 None:intergenic 40.0%
TGGATCGACAAAGGTTGACT+TGG + chr2_1:5339307-5339326 None:intergenic 45.0%
! TTTCGCGTTCTTCCCCATTT+TGG + chr2_1:5339406-5339425 None:intergenic 45.0%
AAGAACGCGAAAAACACGGA+CGG - chr2_1:5339414-5339433 Msa0189880:three_prime_UTR 45.0%
!!! GAAGGTGGTGGTTTTGTCAA+CGG + chr2_1:5339511-5339530 None:intergenic 45.0%
TGTAGTTGACAGAGAAGGAG+AGG + chr2_1:5339533-5339552 None:intergenic 45.0%
AGTGAGGATGATGAAACGGA+GGG + chr2_1:5339568-5339587 None:intergenic 45.0%
AAGTGAGGATGATGAAACGG+AGG + chr2_1:5339569-5339588 None:intergenic 45.0%
AAGAAGGAATGAAACGGAGG+AGG + chr2_1:5339602-5339621 None:intergenic 45.0%
CGAAGCAGTTCGATCGTTAA+CGG - chr2_1:5339767-5339786 Msa0189880:CDS 45.0%
ATCGACGATAATCACCTCGA+AGG - chr2_1:5339798-5339817 Msa0189880:CDS 45.0%
GTAACGAATGAACACCTTCG+AGG + chr2_1:5339815-5339834 None:intergenic 45.0%
! GTTGCTTTGTCTTCAGCGTT+TGG - chr2_1:5339849-5339868 Msa0189880:CDS 45.0%
TTTGTCTTCAGCGTTTGGGA+GGG - chr2_1:5339854-5339873 Msa0189880:CDS 45.0%
! TGGTTTAAGTGGTGGTGTTC+CGG - chr2_1:5340013-5340032 Msa0189880:intron 45.0%
GGTTTGGATGGTGAAGAAGA+TGG - chr2_1:5340058-5340077 Msa0189880:intron 45.0%
TTGGATGGTGAAGAAGATGG+TGG - chr2_1:5340061-5340080 Msa0189880:intron 45.0%
CTGATGAAGCTCTTCAGAGA+TGG - chr2_1:5340096-5340115 Msa0189880:intron 45.0%
! GCTTCGCTTGTTAAACTTGC+AGG - chr2_1:5340173-5340192 Msa0189880:intron 45.0%
TACTTGGCCGAGAATATAGG+TGG - chr2_1:5340357-5340376 Msa0189880:intron 45.0%
TTCGTCTGCGCCAAAATCTT+TGG + chr2_1:5341472-5341491 None:intergenic 45.0%
! TTTTGGCGCAGACGAAATTG+AGG - chr2_1:5341476-5341495 Msa0189880:CDS 45.0%
CAAGATGAAGCAATGCATGC+AGG - chr2_1:5341503-5341522 Msa0189880:CDS 45.0%
CTCATTGATGCTCAATGCTG+GGG + chr2_1:5341592-5341611 None:intergenic 45.0%
CTCTCATTGATGCTCAATGC+TGG + chr2_1:5341594-5341613 None:intergenic 45.0%
! CAGCATTGAGCATCAATGAG+AGG - chr2_1:5341592-5341611 Msa0189880:CDS 45.0%
!! GGAAAGCATTATAGAGGGGT+TGG - chr2_1:5341635-5341654 Msa0189880:CDS 45.0%
GGAACAAGAGAAGCAAGAAC+AGG - chr2_1:5341656-5341675 Msa0189880:CDS 45.0%
AGCGAGCATAACATGATTGG+AGG + chr2_1:5341737-5341756 None:intergenic 45.0%
TCCAATCATGTTATGCTCGC+TGG - chr2_1:5341736-5341755 Msa0189880:CDS 45.0%
ACCAGCGAGCATAACATGAT+TGG + chr2_1:5341740-5341759 None:intergenic 45.0%
GGGGAAGAACGCGAAAAACA+CGG - chr2_1:5339410-5339429 Msa0189880:three_prime_UTR 50.0%
GTTGACAGAGAAGGAGAGGA+AGG + chr2_1:5339529-5339548 None:intergenic 50.0%
GGATGATGAAACGGAGGGTT+TGG + chr2_1:5339563-5339582 None:intergenic 50.0%
GGAGGAAGAGGTAGAAAGTG+AGG + chr2_1:5339584-5339603 None:intergenic 50.0%
GAATGAAACGGAGGAGGAAG+AGG + chr2_1:5339596-5339615 None:intergenic 50.0%
ACACGAAACGACACTGCCAT+CGG - chr2_1:5339684-5339703 Msa0189880:CDS 50.0%
CTTTGTCTTCAGCGTTTGGG+AGG - chr2_1:5339853-5339872 Msa0189880:CDS 50.0%
GTTGAGGCAGTTGGAAGTGA+AGG - chr2_1:5339878-5339897 Msa0189880:CDS 50.0%
GATCAAGGAGAGGGTGAACA+AGG - chr2_1:5339912-5339931 Msa0189880:intron 50.0%
!! AACAAGGTGATGGAGGAGAG+TGG - chr2_1:5339928-5339947 Msa0189880:intron 50.0%
! GTGATGGAGGAGAGTGGTAT+AGG - chr2_1:5339934-5339953 Msa0189880:intron 50.0%
TGGAGGAGAGTGGTATAGGA+TGG - chr2_1:5339938-5339957 Msa0189880:intron 50.0%
GAGAGTGGTATAGGATGGTG+AGG - chr2_1:5339943-5339962 Msa0189880:intron 50.0%
AGTGGTATAGGATGGTGAGG+AGG - chr2_1:5339946-5339965 Msa0189880:intron 50.0%
GTATAGGATGGTGAGGAGGA+GGG - chr2_1:5339950-5339969 Msa0189880:intron 50.0%
! TTTAAGTGGTGGTGTTCCGG+CGG - chr2_1:5340016-5340035 Msa0189880:intron 50.0%
! AATTGGCAGCGGAGTTGGTT+TGG - chr2_1:5340042-5340061 Msa0189880:intron 50.0%
!! GTGAAGAAGATGGTGGAGTG+TGG - chr2_1:5340068-5340087 Msa0189880:intron 50.0%
TGAAGCTCTTCAGAGATGGG+CGG - chr2_1:5340100-5340119 Msa0189880:intron 50.0%
TACTGCTGATCCTCGTTTGC+AGG - chr2_1:5340151-5340170 Msa0189880:intron 50.0%
TAGCTCGAGAATGTGGACGT+AGG - chr2_1:5340391-5340410 Msa0189880:intron 50.0%
TCGAGAATGTGGACGTAGGT+AGG - chr2_1:5340395-5340414 Msa0189880:intron 50.0%
GAATGTGGACGTAGGTAGGT+TGG - chr2_1:5340399-5340418 Msa0189880:intron 50.0%
TGCAGAGAGACCACAATCCT+TGG + chr2_1:5341146-5341165 None:intergenic 50.0%
ATGGCTGCCATTGTTGTGCA+GGG - chr2_1:5341548-5341567 Msa0189880:CDS 50.0%
TTACTGGCCCTGCACAACAA+TGG + chr2_1:5341558-5341577 None:intergenic 50.0%
TTGTTGTGCAGGGCCAGTAA+TGG - chr2_1:5341558-5341577 Msa0189880:CDS 50.0%
CATCAATGAGAGGGCTGAGT+TGG - chr2_1:5341602-5341621 Msa0189880:CDS 50.0%
CACATTGCTCATCCTCTGAC+TGG - chr2_1:5341706-5341725 Msa0189880:CDS 50.0%
AACGCGAAAAACACGGACGG+CGG - chr2_1:5339417-5339436 Msa0189880:three_prime_UTR 55.0%
GAGATTAGGGTTCGGAGGAG+CGG + chr2_1:5339468-5339487 None:intergenic 55.0%
GGGAAGAGATTAGGGTTCGG+AGG + chr2_1:5339473-5339492 None:intergenic 55.0%
! AACGGCGGTGGTGGTGAAAA+GGG + chr2_1:5339493-5339512 None:intergenic 55.0%
!!! GGTGGTGGTTTTGTCAACGG+CGG + chr2_1:5339508-5339527 None:intergenic 55.0%
AGAGAAGGAGAGGAAGGTGG+TGG + chr2_1:5339523-5339542 None:intergenic 55.0%
GACAGAGAAGGAGAGGAAGG+TGG + chr2_1:5339526-5339545 None:intergenic 55.0%
! CTGAGAAAGAGCACGTCCGA+TGG + chr2_1:5339703-5339722 None:intergenic 55.0%
GAGGCAGTTGGAAGTGAAGG+TGG - chr2_1:5339881-5339900 Msa0189880:CDS 55.0%
GCAGTTGGAAGTGAAGGTGG+CGG - chr2_1:5339884-5339903 Msa0189880:CDS 55.0%
!! AAGGTGGCGGAGAGAGATCA+AGG - chr2_1:5339897-5339916 Msa0189880:CDS 55.0%
GCGGAGAGAGATCAAGGAGA+GGG - chr2_1:5339903-5339922 Msa0189880:intron 55.0%
GGAGAGGGTGAACAAGGTGA+TGG - chr2_1:5339918-5339937 Msa0189880:intron 55.0%
! GAGGGTGAACAAGGTGATGG+AGG - chr2_1:5339921-5339940 Msa0189880:intron 55.0%
GGTATAGGATGGTGAGGAGG+AGG - chr2_1:5339949-5339968 Msa0189880:intron 55.0%
TGGAAGGTGAAGGGAGGTGA+GGG - chr2_1:5339990-5340009 Msa0189880:intron 55.0%
GGAGGTGAGGGTGGTTTAAG+TGG - chr2_1:5340002-5340021 Msa0189880:intron 55.0%
GGTGAGGGTGGTTTAAGTGG+TGG - chr2_1:5340005-5340024 Msa0189880:intron 55.0%
TGGTGTTCCGGCGGAGAAAT+TGG - chr2_1:5340025-5340044 Msa0189880:intron 55.0%
GGAGAAATTGGCAGCGGAGT+TGG - chr2_1:5340037-5340056 Msa0189880:intron 55.0%
!! TGGGCGGTGGCGGATAATTT+AGG - chr2_1:5340116-5340135 Msa0189880:intron 55.0%
!! GGGCGGTGGCGGATAATTTA+GGG - chr2_1:5340117-5340136 Msa0189880:intron 55.0%
ACAAGCGAAGCCTGCAAACG+AGG + chr2_1:5340164-5340183 None:intergenic 55.0%
CATGGCTGCCATTGTTGTGC+AGG - chr2_1:5341547-5341566 Msa0189880:CDS 55.0%
!! TGGGGCATCGATGCCATTAC+TGG + chr2_1:5341574-5341593 None:intergenic 55.0%
! GGCTGAGTTGGAGAGAGTGT+TGG - chr2_1:5341614-5341633 Msa0189880:CDS 55.0%
TTGGAGGTTTGGCCAGTCAG+AGG + chr2_1:5341721-5341740 None:intergenic 55.0%
ATTAGGGTTCGGAGGAGCGG+CGG + chr2_1:5339465-5339484 None:intergenic 60.0%
! CAACGGCGGTGGTGGTGAAA+AGG + chr2_1:5339494-5339513 None:intergenic 60.0%
!! GGTTTTGTCAACGGCGGTGG+TGG + chr2_1:5339502-5339521 None:intergenic 60.0%
!!! GGTGGTTTTGTCAACGGCGG+TGG + chr2_1:5339505-5339524 None:intergenic 60.0%
GGCGGAGAGAGATCAAGGAG+AGG - chr2_1:5339902-5339921 Msa0189880:intron 60.0%
! GTGGTGGGGTGGAAGGTGAA+GGG - chr2_1:5339981-5340000 Msa0189880:intron 60.0%
GTGGAAGGTGAAGGGAGGTG+AGG - chr2_1:5339989-5340008 Msa0189880:intron 60.0%
AAGGTGAAGGGAGGTGAGGG+TGG - chr2_1:5339993-5340012 Msa0189880:intron 60.0%
TCCGGCGGAGAAATTGGCAG+CGG - chr2_1:5340031-5340050 Msa0189880:intron 60.0%
TCCGCTGCCAATTTCTCCGC+CGG + chr2_1:5340035-5340054 None:intergenic 60.0%
!! GGCAGCGGAGTTGGTTTGGA+TGG - chr2_1:5340046-5340065 Msa0189880:intron 60.0%
AGCTCTTCAGAGATGGGCGG+TGG - chr2_1:5340103-5340122 Msa0189880:intron 60.0%
TCTTCAGAGATGGGCGGTGG+CGG - chr2_1:5340106-5340125 Msa0189880:intron 60.0%
!! CGGCGGAAGTGGTAGTGGCA+TGG + chr2_1:5339448-5339467 None:intergenic 65.0%
AGGAGCGGCGGAAGTGGTAG+TGG + chr2_1:5339453-5339472 None:intergenic 65.0%
!! CAGCGTTTGGGAGGGCGTTG+AGG - chr2_1:5339862-5339881 Msa0189880:CDS 65.0%
!! TGGGAGGGCGTTGAGGCAGT+TGG - chr2_1:5339869-5339888 Msa0189880:CDS 65.0%
GGATGGTGAGGAGGAGGGTG+TGG - chr2_1:5339955-5339974 Msa0189880:intron 65.0%
! TGGTGAGGAGGAGGGTGTGG+AGG - chr2_1:5339958-5339977 Msa0189880:intron 65.0%
! GGTGAGGAGGAGGGTGTGGA+GGG - chr2_1:5339959-5339978 Msa0189880:intron 65.0%
! AGGAGGGTGTGGAGGGTGGT+GGG - chr2_1:5339966-5339985 Msa0189880:intron 65.0%
!! GGTGGTGGGGTGGAAGGTGA+AGG - chr2_1:5339980-5339999 Msa0189880:intron 65.0%
GTGGGGTGGAAGGTGAAGGG+AGG - chr2_1:5339984-5340003 Msa0189880:intron 65.0%
GTTCGGAGGAGCGGCGGAAG+TGG + chr2_1:5339459-5339478 None:intergenic 70.0%
! GAGGAGGAGGGTGTGGAGGG+TGG - chr2_1:5339962-5339981 Msa0189880:intron 70.0%
! GAGGAGGGTGTGGAGGGTGG+TGG - chr2_1:5339965-5339984 Msa0189880:intron 70.0%
! GGAGGGTGTGGAGGGTGGTG+GGG - chr2_1:5339967-5339986 Msa0189880:intron 70.0%
!! GTGGAGGGTGGTGGGGTGGA+AGG - chr2_1:5339974-5339993 Msa0189880:intron 70.0%
!! GGGTGTGGAGGGTGGTGGGG+TGG - chr2_1:5339970-5339989 Msa0189880:intron 75.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 5339303 5342067 5339303 ID=Msa0189880;Name=Msa0189880
chr2_1 mRNA 5339303 5342067 5339303 ID=Msa0189880-mRNA-1;Parent=Msa0189880;Name=Msa0189880-mRNA-1;_AED=0.01;_eAED=0.01;_QI=105|1|1|1|1|1|2|264|379
chr2_1 exon 5339303 5339919 5339303 ID=Msa0189880-mRNA-1:exon:2430;Parent=Msa0189880-mRNA-1
chr2_1 exon 5341176 5342067 5341176 ID=Msa0189880-mRNA-1:exon:2429;Parent=Msa0189880-mRNA-1
chr2_1 five_prime_UTR 5341963 5342067 5341963 ID=Msa0189880-mRNA-1:five_prime_utr;Parent=Msa0189880-mRNA-1
chr2_1 CDS 5341176 5341962 5341176 ID=Msa0189880-mRNA-1:cds;Parent=Msa0189880-mRNA-1
chr2_1 CDS 5339567 5339919 5339567 ID=Msa0189880-mRNA-1:cds;Parent=Msa0189880-mRNA-1
chr2_1 three_prime_UTR 5339303 5339566 5339303 ID=Msa0189880-mRNA-1:three_prime_utr;Parent=Msa0189880-mRNA-1
Gene Sequence

>Msa0189880

ATGGGGAAGAACGCGAAAAACACGGACGGCGGCGACGCCATGCCACTACCACTTCCGCCGCTCCTCCGAACCCTAATCTCTTCCCTTTTCACCACCACCGCCGTTGACAAAACCACCACCTTCCTCTCCTTCTCTGTCAACTACAAACTCATCCAAACCCTCCGTTTCATCATCCTCACTTTCTACCTCTTCCTCCTCCGTTTCATTCCTTCTTTTTTCTTCAATGAAAACTACGAACACTCCACCATCAAAACGCAGAATAAGTTCACACACGATACACGAAACGACACTGCCATCGGACGTGCTCTTTCTCAGCTTCTTTCTGCATTAAACGATATTCCAGTTAGTTCAAGAAAATACGAAGCAGTTCGATCGTTAACGGAGAAGATTATCGACGATAATCACCTCGAAGGTGTTCATTCGTTACGTGAAGTTAATTGTGTTGCTTTGTCTTCAGCGTTTGGGAGGGCGTTGAGGCAGTTGGAAGTGAAGGTGGCGGAGAGAGATCAAGGAGAGGGTGAACAAGGTGATGGAGGAGAGTGGTATAGGATGGTGAGGAGGAGGGTGTGGAGGGTGGTGGGGTGGAAGGTGAAGGGAGGTGAGGGTGGTTTAAGTGGTGGTGTTCCGGCGGAGAAATTGGCAGCGGAGTTGGTTTGGATGGTGAAGAAGATGGTGGAGTGTGGTTGTGCTGATGAAGCTCTTCAGAGATGGGCGGTGGCGGATAATTTAGGGTTTCTTGCTCTTACTGCTGATCCTCGTTTGCAGGCTTCGCTTGTTAAACTTGCAGCATTTCTGTTCAAAGAAGCCAAAGATTTTGGCGCAGACGAAATTGAGGAAAGCAAGATGAAGCAATGCATGCAGGTAAAGTTAAAGATGTTACAGACATGGCTGCCATTGTTGTGCAGGGCCAGTAATGGCATCGATGCCCCAGCATTGAGCATCAATGAGAGGGCTGAGTTGGAGAGAGTGTTGGAAAGCATTATAGAGGGGTTGGAACAAGAGAAGCAAGAACAGGTTCTTTCTCTTTGGCTCCACCATTTTACACATTGCTCATCCTCTGACTGGCCAAACCTCCAATCATGTTATGCTCGCTGGTGCTGTAAATCTCGCAAGCAGTTGCTGCTTTCGAGTGAAAATTGA
Protein sequence

>Msa0189880

MGKNAKNTDGGDAMPLPLPPLLRTLISSLFTTTAVDKTTTFLSFSVNYKLIQTLRFIILTFYLFLLRFIPSFFFNENYEHSTIKTQNKFTHDTRNDTAIGRALSQLLSALNDIPVSSRKYEAVRSLTEKIIDDNHLEGVHSLREVNCVALSSAFGRALRQLEVKVAERDQGEGEQGDGGEWYRMVRRRVWRVVGWKVKGGEGGLSGGVPAEKLAAELVWMVKKMVECGCADEALQRWAVADNLGFLALTADPRLQASLVKLAAFLFKEAKDFGADEIEESKMKQCMQVKLKMLQTWLPLLCRASNGIDAPALSINERAELERVLESIIEGLEQEKQEQVLSLWLHHFTHCSSSDWPNLQSCYARWCCKSRKQLLLSSEN*